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371 results on '"Sun, Yuanming"'

7. Generation of functional single-chain fragment variable from hybridoma and development of chemiluminescence enzyme immunoassay for determination of total malachite green in tilapia fish

Author

Dong, Jiexian, Li, Zhenfeng, Wang, Yu, Jin, Maojun, Shen, Yudong, Xu, Zhenlin, El-Aty, AM Abd, Gee, Shirley J, Hammock, Bruce D, Sun, Yuanming, and Wang, Hong

Subjects
Agricultural, Veterinary and Food Sciences, Food Sciences, Biotechnology, Alkaline Phosphatase, Animals, Antibodies, Monoclonal, Drug Residues, Fish Products, Hybridomas, Immunoenzyme Techniques, Luminescence, Recombinant Fusion Proteins, Rosaniline Dyes, Single-Chain Antibodies, Tilapia, Database-assisted sequence analysis, ScFv-AP based immunoassay, Malachite green, Residue determination, Tilapia fish
Abstract

To determine malachite green (MG) and its major metabolite, leucomalachite green (LMG) residual levels in tilapia fish, chemiluminescent enzyme immunoassay (CLEIA) was developed based on a single-chain variable fragment (scFv)-alkaline phosphatase (AP) fusion protein. At first, VH and VL gene sequences were cloned from hybridoma cell lines secreting monoclonal antibody against LMG, and then thoroughly by database-assisted sequence analysis. Finally, the productive VH and VL were assembled to an intact scFv sequence and engineered to produce scFv-AP fusion protein. The fusion protein was further identified as a bifunctional reagent for immunoassay, then a sensitive one-step CLEIA against LMG was developed with a half-maximal inhibitory concentration (IC50) and limit of detection (LOD) of 1.3 and 0.04 ng/mL, respectively. The validation results of this novel competitive CLEIA was in line with those obtained by classical HPLC method for determination of total MG in spiked and field incurred samples.

Published
2021

12. Development of an Open Droplet Microchannel-Based Magnetosensor for Immunofluorometric Assay of Trimethoprim in Chicken and Pork Samples with a Wide Linear Range

Author

Wang, Lei, primary, Zhang, Yongyi, additional, Zeng, Dao-Ping, additional, Zhu, Yuxian, additional, Ling, Zhizhou, additional, Wang, Yu, additional, Yang, Jinyi, additional, Wang, Hong, additional, Xu, Zhen-Lin, additional, Tian, Yuanxin, additional, Sun, Yuanming, additional, and Shen, Yu-Dong, additional

Published
2024
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18. Ultrasmall Glutathione-Protected Gold Nanoclusters as Next Generation Radiotherapy Sensitizers with High Tumor Uptake and High Renal Clearance

Author

Zhang, Xiao-Dong, Luo, Zhentao, Chen, Jie, Song, Shasha, Yuan, Xun, Shen, Xiu, Wang, Hao, Sun, Yuanming, Gao, Kai, Zhang, Lianfeng, Fan, Saijun, Leong, David Tai, Guo, Meili, and Xie, Jianping

Subjects
Physics - Medical Physics, Physics - Biological Physics
Abstract

Radiotherapy is often the most straightforward first line cancer treatment for solid tumors. While it is highly effective against tumors, there is also collateral damage to healthy proximal tissues especially with high doses. The use of radiosensitizers is an effective way to boost the killing efficacy of radiotherapy against the tumor while drastically limiting the received dose and reducing the possible damage to normal tissues. Here, we report the design and application of a good radiosensitizer by using ultrasmall gold nanoclusters with a naturally occurring peptide (e.g., glutathione or GSH) as the protecting shell. The GSH coated gold nanoclusters can escape the RES absorption, leading to a good tumor uptake (8.1% ID/g at 24 h post injection). As a result, the as-designed Au nanoclusters led to a strong enhancement for radiotherapy, as well as a negligible damage to normal tissues. After the treatment, the ultrasmall gold nanoclusters can be efficiently cleared by the kidney, thereby avoiding potential long term side effects caused by the accumulation of gold atoms in the body. Our data suggest that the ultrasmall peptide protected Au nanoclusters are a promising radiosensitizer for cancer radiotherapy., Comment: 15 Pages, 6 Figures, Scientific Reports 5, 2015

Published
2015
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26. Ultrasmall Au10-12(SG)10-12 Nanomolecules for High Tumor Specificity and Cancer Radiotherapy

Author

Zhang, Xiao-Dong, Luo, Zhentao, Chen, Jie, Shen, Xiu, Song, Shasha, Sun, Yuanming, Fan, Saijun, Fan, Feiyue, Leong, David Tai, and Xie, Jianping

Subjects
Physics - Biological Physics, Physics - Medical Physics, Quantitative Biology - Tissues and Organs
Abstract

Radiosensitizers can increase the local treatment efficacy under a relatively low and safe radiation dose, thereby facilitating tumor eradication and minimizing side effects. Here, we report a new class of radiosensitizers that contain several gold (Au) atoms embedded inside a peptide shell (e.g., Au10-12(SG)10-12) and can achieve ultrahigh tumor uptake (10.86 SUV at 24 h post injection) and targeting specificity, efficient renal clearance, and high radiotherapy enhancement., Comment: 21 pages, 11 figures

Published
2014
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42. Immunological activity and gut microbiota modulation of pectin from kiwano (Cucumis metuliferus) peels

Author

Zhu, Minqian, Song, Ya, Martínez-Cuesta, M. Carmen, Peláez, Carmen, Li, Enru, Requena, Teresa, Wang, Hong, Sun, Yuanming, Guangdong Science and Technology Department, National Key Research and Development Program (China), Ministerio de Ciencia e Innovación (España), Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), and Agencia Estatal de Investigación (España)

Subjects
Health (social science), Kiwano, Immune-enhancing, Cucumis metuliferus, Plant Science, kiwano, pectin, immune-enhancing, microbiota simulation, Health Professions (miscellaneous), Microbiology, Microbiota simulation, Pectin, Food Science
Abstract

This article belongs to the Special Issue Analysis, Detection Technology and Safety Risk Assessment of Food Contaminants., For developing the recycling of fruit by-products from kiwano, a polysaccharide was extracted from kiwano (Cucumis metuliferus) peels, namely Cucumis metuliferus peels polysaccharide (CMPP), with the aim of investigating the potential beneficial effects. The composition of polysaccharides was analyzed by chemical methods. RAW264.7 macrophages cells and the microbiota dynamics simulator (BFBL gut model) were used for in vitro study. The result showed that CMPP mainly consists of glucuronic acid, arabinose, galactose and rhamnose. By intervening with RAW264.7 cells, CMPP promoted cell proliferation and showed immune-enhancing activity, which significantly (p < 0.05) induced the release of nitric oxide (NO), tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) at a concentration of 50 μg/mL. In addition, CMPP had an impact on the composition of the gut bacteria, increasing the growth of Akkermansia, Bacteroides, Bifidobacterium, Feacalibacterium, and Roseburia. During the intake period, acetic, butyric and propionic acids were all increased, especially (p < 0.05) in the descending colon. Moreover, a decrease in ammonia concentration (10.17 ± 0.50 mM in the ascending colon, 13.21 ± 1.54 mM in the transverse colon and 13.62 ± 0.45 mM in the descending colon, respectively) was observed. In summary, CMPP can be considered as a pectin, showed immunological activity and function of gut microbiota modulation. This study could be the scientific basis of developing kiwano peels as beneficial to human health., This research was funded by the Lingnan Modern Agricultural Science and Technology Guangdong Laboratory independent scientific research project (NZ2021032), the Key Area R&D Program of Guangdong Province (NO. 2019B020211002), the Guangdong Province Universities and Colleges Pearl River Scholar Funded Scheme (2017), and the International Cooperation Program of SCAU (NO. 2019SCAUGH03). This research was funded by the Spanish Ministry of Science and Innovation (grant numbers AGL2016-75951-R and PID2019-106071RB-I00)

Published
2022

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