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15 results on '"Sung, MinJi"'

5. Development of a rapid and sensitive real-time diagnostic assay to detect and quantify Aphanomyces invadans, the causative agent of epizootic ulcerative syndrome.

Author

Ho, Diem Tho, Kim, Nameun, Lee, Yoonhang, Yun, Dongbin, Sung, MinJi, Mansour, El-Matbouli, Pradhan, P. K., Sood, Neeraj, Kim, Wi-Sik, Park, Chan-Il, Kim, Ki Hong, and Kim, Do-Hyung

Subjects
POLYMERASE chain reaction, WATER sampling, DISEASE management, DETECTION limit, ANIMAL health
Abstract

The oomycete Aphanomyces invadans causes epizootic ulcerative syndrome (EUS), a World Organization for Animal Health (WOAH)-listed disease that has seriously impacted a wide range of fish worldwide. Currently, only three conventional polymerase chain reaction (PCR) assays are recommended for the detection of A. invadans. The robust quantitative PCR (qPCR) assay has recently become more important due to its highly accurate nature and the applicability of qPCR-based environmental DNA (eDNA) detection in the monitoring of pathogens in aquatic environments. Therefore, in this study, we developed a novel TaqMan probe-based qPCR method to sensitively and quantitatively detect A. invadans. The assay limit of detection was determined using 10-fold serial dilutions of linearized A. invadans plasmid. Assay sensitivity was assessed in the presence of interfering substances and compared to three WOAH-listed primers using the mycelia and zoospores of A. invadans with and without fish muscle tissue. The assay specificity was also theoretically and experimentally assessed against other oomycetes, fish muscle tissue, and water samples. The assay's repeatability and reproducibility were determined. In this study, the limit of detection of the developed assay was 7.24 copies of A. invadans genomic DNA per reaction (95% confidence interval (CI): 2.75 to 19.05 copies/reaction). The assay showed the same sensitivity in the presence of other substances. Compared to the WOAH-recommended PCR assays, this assay had 10-times higher sensitivity for all tested samples. There were no cross-reactions with other closely related oomycetes, fish muscle, or water samples, indicating that the assay was highly specific for A. invadans. The repeatability and reproducibility tests showed little variation, ranging from 0.1–0.9% and 0.04–1.1%, respectively, indicating the high consistency, repeatability, and reliability of the developed assay. This highly rapid, sensitive, specific, and consistent EUS qPCR assay would be of importance in transboundary disease management and the monitoring of pathogens in aquatic environments. [ABSTRACT FROM AUTHOR]

Published
2023
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6. Hyperglycemia and Hypoglycemia Are Associated with In-Hospital Mortality among Patients with Coronavirus Disease 2019 Supported with Extracorporeal Membrane Oxygenation

Author

Son, Kuk Hui, primary, Kim, Woong-Han, additional, Kwak, Jae Gun, additional, Choi, Chang-Hyu, additional, Lee, Seok In, additional, Ko, Ui Won, additional, Kim, Hyoung Soo, additional, Lee, Haeyoung, additional, Chung, Euy Suk, additional, Kim, Jae-Bum, additional, Jang, Woo Sung, additional, Jung, Jae Seung, additional, Kim, Jieon, additional, Yoon, Young Kyung, additional, Song, Seunghwan, additional, Sung, Minji, additional, Jang, Myung Hun, additional, Kim, Young Sam, additional, Jeong, In-Seok, additional, Kim, Do Wan, additional, Kim, Tae Yun, additional, Kim, Soon Jin, additional, Kim, Su Wan, additional, Hong, Joonhwa, additional, and An, Hyungmi, additional

Published
2022
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11. Beneficial Effect of Chloroquine and Amodiaquine on Type 1 Diabetic Tubulopathy by Attenuating Mitochondrial Nox4 and Endoplasmic Reticulum Stress

Author

Kang, Jun Mo, primary, Lee, Hyun-Seob, additional, Kim, Junghyun, additional, Yang, Dong Ho, additional, Jeong, Hye Yun, additional, Lee, Yu Ho, additional, Kim, Dong-Jin, additional, Park, Seon Hwa, additional, Sung, MinJi, additional, Kim, Jaehee, additional, An, Hyun-Ju, additional, Lee, Sang Ho, additional, and Lee, So-Young, additional

Published
2020
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