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This study investigated the effect of probiotic supplementation into diets on blood urea nitrogen (BUN) and plasma creatinine of broiler chickens. The probiotic used in this study is a commercial probiotic powder containing 3 dominant strains, namely Candida ethanolica, Monascus fumeus, and Bacillus subtilis. One hundred broiler chickens were randomly assigned to four treatments. The treatments were the percentage of probiotic supplementation on basal diet: PP0 (control/basal diet with no probiotic supplementation; PP025 (basal diet with 0.25% probiotic); PP050 (basal diet with 0.50% probiotic); PP075 (basal dietwith0.75% probiotic). The probiotic powder in respective treatment was calculated based on amount of basal diet supplementation. The calculated amount of probiotic then mixed to the basal diet prior to supplied to the animals. The results showed that there was no significant difference in blood urea nitrogen (BUN) and plasma creatinine in any probioticfed birds compared to control group. However, there is a tendency of decrease in creatinine level in PP050, suggesting a lower rate of muscle expansion and kidneys function, although the difference was not significant. BUN and plasma creatinine were associated with the functionality and health of kidneys. In conclusion, the probiotic supplementation in the diet give neither benefit nor damage to renal health and functionality of broiler chickens. [ABSTRACT FROM AUTHOR]