1. Herpes Simplex Virus Type 2 UL24 Gene Is a Virulence Determinant in Murine and Guinea Pig Disease Models
- Author
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Robert J. Visalli, Min Guo, Deborah Long, Jacek Kowalski, Donna Tummolo, Robert J. Natuk, David K. C. Cooper, Susan J. Blakeney, Timothy J. Zamb, Seema S Gangolli, and Joanne DeStefano
- Subjects
viruses ,Herpesvirus 2, Human ,Guinea Pigs ,Molecular Sequence Data ,Immunology ,Virulence ,Genome, Viral ,Virus Replication ,medicine.disease_cause ,Thymidine Kinase ,Microbiology ,Herpesviridae ,Virus ,Guinea pig ,Mice ,Viral Proteins ,Virology ,Alphaherpesvirinae ,medicine ,Animals ,Cytopathic effect ,Mice, Inbred BALB C ,Base Sequence ,biology ,Lethal dose ,Herpes Simplex ,biology.organism_classification ,Disease Models, Animal ,Herpes simplex virus ,Insect Science ,Pathogenesis and Immunity ,Female - Abstract
A herpes simplex virus type 2 (HSV-2) UL24 β-glucuronidase (UL24-βgluc) insertion mutant was derived from HSV-2 strain 186 via standard marker transfer techniques. Cell monolayers infected with UL24-βgluc yielded cytopathic effect with syncytium formation. UL24-βgluc replicated to wild-type viral titers in three different cell lines. UL24-βgluc was not virulent after intravaginal inoculation of BALB/c mice in that all inoculated animals survived doses up to 400 times the 50% lethal dose (LD 50 ) of the parental virus. Furthermore, few UL24-βgluc-inoculated mice developed any vaginal lesions. Intravaginal inoculation of guinea pigs with UL24-βgluc at a dose equivalent to the LD 50 of parental virus (≈5 × 10 3 PFU) was not lethal (10/10 animals survived). Although genital lesions developed in some UL24-βgluc-inoculated guinea pigs, both the overall number of lesions and the severity of disease were far less than that observed for animals infected with parental strain 186.
- Published
- 2005