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Near-infrared (NIR) spectroscopic methods for measuring degradation products, including total polar materials (TPMs) and free fatty acids (FFAs), in soy-based frying oil used for frying various foods have been successfully developed. Calibration models were developed using forward stepwise multiple linear regression (FSMLR) and partial least-squares (PLS) regression techniques and then tested with an independent set of validation samples. The results show that the quality of oil used for frying different foods can be measured with a single model. First-derivative treatments improved results for TPM measurement. In addition, PLS models gave better prediction results than FSMLR models. For PLS models, the best correlations (r) between the NIR -predicted data and the chemical method data for TPMs and FFAs in oils were 0.995 and 0.981, respectively. For FSMLR models, the best r values for TPMs and FFAs in oils were 0.993 and 0.963, respectively.

The floss and oil of the common milkweed ( Asclepias syriaca L.) seeds are currently used to produce comforters/pillows and skin care products, respectively. As an outcome of these products, copious quantities co-products (pressed seed meal and pod biomass) are disposed of each year despite the presence of potential health benefiting lipids. The objective of this project was to determine the feasibility of developing the lipid fraction from of these co-products for the fast growing dietary human health market. Although certain types of lipids were affected by the extraction solvent used (hexane and diethyl ether) as were overall amounts, analysis of the each extract showed novel lipid profiles with several potential health benefiting agents present at levels comparable to or exceeding those present in other typically consumed dietary oils or food systems (vitamin E, carotenoids, sterols and unsaturated free fatty acids, particularly the both omega 7-fatty acids).

Malolactic fermentation (MLF), the conversion of malate to lactate, is an important process leading to the deacidification of hard apple cider. MLF is dependent on the levels of inhibitory factors such as sulfur dioxide and ethanol. To assess the effect of these 2 factors on MLF, hard apple cider was produced from pasteurized, unfiltered apple cider (Malus domestica cvs Red Delicious, Golden Delicious, Braeburn, and Fuji). Apple cider was treated with 2 levels of sulfur dioxide (50 and 80 ppm) and then fermented using Saccharomyces cerevisiae montrachet. After the primary fermentation, 1 set of the samples remained unadjusted and 100% ethyl alcohol was used to adjust other sets of samples to 7%, 9%, or 11% (v/v) ethanol. Following the ethanol adjustment, Oenococcus oeni MCW was used to initiate the MLF in half of the samples. Cider parameters monitored throughout the fermentations included organic acid content, titratable acidity, pH, ethanol production, and sugar content. Since samples containing either sulfur dioxide level had similar sugar utilization rates and ethanol production it was concluded that sulfur dioxide had no effect on the primary fermentation. Sulfur dioxide content was shown to have an impact on MLF. There was no difference in the rate of malic acid consumption, but lactic acid production was faster in the 50-ppm sulfur dioxide samples. MLF was not inhibited by ethanol content.

Antiproliferative properties of lipids extracted from grain sorghum (GS) dry distiller's grain (DDG) were analyzed to determine the feasibility of developing GS coproducts as a source for human health dietary ingredients. The lipid extract of GS-DDG was delivered to human colon carcinoma (Caco-2) cells by solubilizing 0-1000 microg/mL of GS-DDG lipids in 100 microg/mL increments with micelles. A significant reduction in cell viability (25-50%) resulted at treatment levels of 400-1000 microg/mL GS-DDG lipids (p0.05). Alternatively, total protein levels of cells treated with 400, 500, and 600 microg/mL of GS-DDG lipid were not significantly different from the control, indicating cell growth during the treatment period. Total cell counts for the control were not significantly different from the GS-DDG lipid treated cells, but dead cell counts increased by approximately 10% for the latter sample with a concomitant increase of the intercellular protein lactate dehydrogenase leakage (30-40%) in the medium. Preliminary analysis by the fluorescence-activated cell method (FACs) demonstrated that nonviable cells were in either the early apoptotic, late apoptotic, or necrotic stage post-treatment with 400, 500, and 600 microg/mL GS-DDG lipids. Physiochemical characterization of the GS-DDG lipids used for the antiproliferation study showed the presence of vitamin E (predominantly gamma-tocopherol), triacylglycerides (predominantly linoleic acid), policosanols, aldehydes, and sterols (predominantly campesterol and stigmasterol), each of which or as synergistic/additive group of constituents may be responsible for the antiproliferative effect.

Grain sorghum is a rich source of phytochemicals. In this study, male hamsters were fed AIN-93M diets supplemented with a hexane-extractable lipid fraction from sorghum distillers dried grains with solubles (DDGS). Diets contained 0.0%, 0.5%, 1.0%, and 5.0% (w/w) DDGS lipid extract. After 4 wk, the 5.0% DDGS lipids group had significantly lower plasma non-HDL cholesterol and liver esterified cholesterol concentration. Faecal neutral sterol (i.e., cholesterol) excretion was significantly higher in the 5.0% DDGS lipids group compared to the other treatments (66% higher compared to controls). Bile acid excretion was not affected by DDGS lipid intake. Faecal cholesterol excretion was negatively correlated with liver cholesterol concentration ( r = −0.97, P = 0.026), and liver cholesterol concentration was directly correlated with plasma total cholesterol concentration ( r = 0.96, P = 0.041). Thus, lipid extract of sorghum DDGS exhibited cholesterol-lowering properties due, at least in part, to increased cholesterol excretion from the body and could provide health benefits when incorporated into human diets.

The effects of 24 h supplementation of human colon carcinoma cells (Caco-2) with isoflavones, genistein, and daidzein and their activities against oleic acid hydroperoxide mediated oxidative stress were investigated. Genistein, at 25, 50, and 100 microM, and daidzein, at 25 and 50 microM, did not induce cell injury to Caco-2 cells. Both compounds reduced cell injury and DNA damage mediated by 5 microM oleic acid hydroperoxides in Caco-2 cells. The effects of genistein and daidzein on antioxidant enzymes were dependent upon the compound and its concentration.

The objectives of this study were to identify and quantify lipid classes extracted from the stalk, leaves, and the whole kernels of nine different sorghum parent lines. All extractions were performed with a Soxtec using n-hexane as the extraction solvent. Analytical methods including high-performance liquid chromatography (HPLC), gas chromatography (GC), and thin-layer chromatography (TLC) were used to identify and quantify the extracted lipid classes: triacylglycerides (TAG), diglycerides (DG), policosanols (PA), free sterols (FS), phytosterols (PS), free fatty acids (FFA), tocopherols (T), and fatty aldehydes. Overall, extracts from the leaves had higher amounts of soluble matter. Extracts from leaves contained more alcohols and sterols, whereas extracts from the stalks and the whole kernels contained the greatest amount of free fatty acids and fatty aldehydes, respectively. Trace amounts of tocopherol were detected by HPLC in all 27 samples. The lipid profiles of extracts from the whole kernel...

Total yields and compositions of sorghum dried distillers grains with solubles (DDGS) lipids obtained by supercritical CO 2 (SC-CO 2 ) extraction were compared with those obtained by recirculated solvent extraction (RSE) with hexane. The total yield of lipids obtained by SC-CO 2 extraction at 27.5 MPa and 70 °C was 150 g lipids/kg DDGS, while the yield obtained by RSE with hexane at 69 °C was only 85 g lipids/kg DDGS. The contents of four high-value compounds, i.e., policosanols, phytosterols, free fatty acids (FFA) and tocols, in the lipids obtained by SC-CO 2 extraction were 31.2, 15.6, 155.3 and 0.50 mg/g at 27.5 MPa and 70 °C, compared to 26.6, 9.6, 57.3 and 0.03 mg/ g for RSE with hexane at 69 °C. The profiles of phytosterols and FFA in the sorghum DDGS lipids were relatively independent of the extraction methods and operating conditions.

A rapid near-infrared (NIR) spectroscopic method for measuring degradation products in frying oils, including total polar materials (TPMs) and free fatty acids (FFAs), has been developed. Calibration models were developed using both forward stepwise multiple linear regression (FSMLR) and partial least-squares (PLS) regression techniques and then tested with two independent sets of validation samples. Derivative treatments had limited usefulness, especially in the longer (1100-2500 nm) wavelength region. When using a wavelength region of 700-1100 nm, PLS models gave improved results compared to FSMLR models. The best correlations (r) between the NIR and wet chemical methods for TPM and FFA were 0.983 and 0.943, respectively. In the longer wavelength region (1100-2500 nm), FSMLR models were as good or better than PLS models. The best correlations for TPM and FFA obtained in this region were 0.999 and 0.983, respectively.

Studies were conducted to evaluate the cell damage caused by exposing human colon carcinoma cells, Caco-2, to hydrogen peroxide at concentrations varying from 0 to 250 microM for 30 min. Evaluation of cell viability, as measured by trypan blue dye exclusion test, showed that the loss of viability was5% at concentrations up to 250 microM hydrogen peroxide. Cell membrane damage and DNA damage as measured by the leakage of lactate dehydrogenase and the comet assay, respectively, were significantly high at concentrations100 microM hydrogen peroxide compared to those of the control. Antioxidant mechanisms in Caco-2 cells were evaluated by measuring catalase, superoxide dismutase, and glutathione peroxidase activities. Catalase activities remained constant in cells treated with 50-250 microM hydrogen peroxide. Superoxide dismutase activity decreased, whereas glutathione peroxidase activity increased in cells treated with H(2)O(2) concentrations of50 microM. This study showed that with increasing hydrogen peroxide concentration, cell membrane leakage and DNA damage increased, whereas the three antioxidant enzymes responded differently, as shown by mathematical models.

Oral ingestion of pesticides can be a major exposure route. These compounds are frequently consumed in the presence of triacylglycerides, which are then hydrolyzed to free fatty acids. The purpose of this work was to examine the effect of two common fatty acids, palmitic (PA) and oleic (OA) acids, and the biological emulsifier sodium taurocholate (TC) on the absorption of three herbicides (trifluralin, alachlor and atrazine) by Caco-2 cell monolayers. Trifluralin's absorption was enhanced (p < 0.05) in the presence of OA whereas the greatest absorption of atrazine and alachlor occurred with PA and the control media, respectively. Trifluralin had significantly lower absorption through the monolayer than either alachlor or atrazine (p < 0.001). A mass balance study demonstrated that trifluralin accumulated within the cell monolayer (13.85% of the donor after 3 h of exposure), but alachlor and atrazine (1.27% and 0.85%, respectively) did not. This response was linear with time (21.89% trifluralin after 6 h of exposure), and demonstrated the potential for continued release of trifluralin after source removal. These experiments demonstrated that fatty acids and an emulsifier can influence absorption of herbicides across small intestinal epithelium.

Grain sorghum can be a major source of policosanols, long-chained alcohols, that have beneficial physiological activities. Sorghum dried distillers grains (DDG), a by-product of ethanol production from grain sorghum, contain a large amount of policosanols. Content and composition of policosanols in long-chained lipids extracted from grain sorghum kernels and DDG were determined. Long-chained lipids were extracted using hot hexane or hot ethanol. The major components of the long-chained lipids extracted from grain sorghum kernels, as determined using HPLC, were policosanols (37–44%), aldehydes (44–55%), and acids (4–5%). Long-chained lipids from DDG contained 52% policosanols, 23% aldehydes, 6.4% acids, and 17% wax esters/steryl esters. Composition of policosanols in DDG matched the composition in grain sorghum kernels, as determined by gas chromatography, even though the content of policosanols in DDG was greater than the content in grain sorghum kernels. Policosonal composition ranges were 0–1% ...

Grain sorghum (Sorghum bicolor) wax is composed mainly of aldehydes, alcohols, and acids. Aldehydes, comprising about one-half of the wax, are readily converted to acids in presence of air. In this study, whole sorghum wax and an aldehyde fraction from sorghum wax were subjected to oxidative conditions. Changes in the major components and thermal transition temperatures were determined using HPLC and differential scanning calorimeter (DSC), respectively. The aldehyde fraction was oxidized markedly to acids over 4 months in storage at room temperature. Acid content, in the fraction, was initially 5–7% and increased to 42– 51% after 135 days in storage. Consequently, thermal transition apex and end temperatures of the fraction, which were initially 73–74 and 76–77 °C, respectively, increased to 80–81 and 83–85 °C, respectively, after 135 days. Whole sorghum wax, composed initially of 55% aldehydes, 37% alcohols, and 7% acids, slightly increased acid level to 8–12% during storage over 5 months under various conditions. Thermal transition temperatures of the wax changed little over all storage conditions during 5 months of storage with 83–84 °C for apex temperatures and 86–87 °C for end temperatures.

Previous work in our laboratory demonstrated that soybean oil oxidation, expressed as PV, can be determined using NIR transmission spectroscopy as an alternative to the official AOCS iodometric titration method. In the present study, a comparison of four peroxide analytical methods was conducted using oxidized soybean oil. The methods included the official AOCS iodometric titration, the newly developed NIR method, the PeroxySafe™ kit, and a ferrous xylenol orange (FOX) method, the latter two being colorimetric methods based on oxidation of iron. Five different commercially available soybean oils were exposed to fluorescent light to obtain PV levels of 0–20 meq/kg; periodic sampling was done to ensure having representative samples throughout the designated range. A total of 46 oil samples were analyzed. Statistical analysis of the data showed that the correlation coefficient (r) and standard deviation of differences (SDD) between the standard titration and NIR methods were r=0.991, SDD=0.72 meq/kg; between titration and the PeroxySafe™ kit were r=0.993, SDD=0.56 meq/kg; and between the standard titration and FOX method were r=0.975, SDD=2.3 meq/kg. The high correlations between the titration, NIR, and PeroxySafe™ kit data indicated that these methods were equivalent.

NIR spectroscopy was used successfully in our laboratory to monitor oxidation levels in vegetable oils. Calibration models were developed to measure PV in both soy and corn oils, using partial least squares (PLS) regression and forward stepwise multiple linear regression, from NIR transmission spectra. PV can be measured successfully in both corn and soy oils using a single calibration. The most successful calibration was based on PLS regression of first derivative spectra. When this calibration was applied to validation sample sets containing equal numbers of corn and soy oil samples, with PV ranging from 0 to 20 meq/kg, a correlation coefficient of 0.99 between titration and NIR values was obtained, with a standard error of prediction equal to 0.72 meq/kg. For both types of oil, changes occurred in the 2068 nm region of the NIR spectra as oxidation levels increased. These changes appear to be associated with the formation of hydroperoxides during oxidation of the oils.

The purpose of this study was to determine the effectiveness of electron beam irradiation at doses of 1.0 and 1.8 kGy on the elimination of bacteria from boneless, skinless chicken breasts without significantly altering product quality. Microbial testing was conducted in triplicate using a whole carcass rinse method with each nonirradiated control group and an irradiation treatment group consisting of 10 samples. Results indicated that mean counts for coliforms, generic Escherichia coli, and psychrotrophs were 3.13, 3.26, and 1.92 log10 cfu/200 mL rinsate, respectively, in the control samples. However, these populations were not detected after the samples were irradiated with 1.0 or 1.8 kGy. Mean count of 4.60 log10 cfu/200 mL rinsate was detected for aerobic bacteria in the control samples. Irradiation doses of 1.0 and 1.8 kGy reduced the levels to 2.23 and 1.62 log10 cfu/200 mL rinsate, respectively. Irradiation also rendered the fillets free of Salmonella and Campylobacter. Consumer taste panels (product stored for 0, 14, and 28 d at 0 C) indicated that, at Day 0, there were no differences among controls and treatment groups for any of the quality attributes tested. At Day 14, texture and flavor attributes were lower for the irradiated groups. At Day 28, samples irradiated with 1.0 and 1.8 kGy were less desirable with decreased texture, flavor, and overall acceptability. Degree of lipid oxidation also increased as storage time and level of irradiation increased. Irradiated samples also had higher a* values, indicating they were pinker in color.

Improved knowledge of the properties, composition, and analysis of grain sorghum wax would assist in efforts for industrial application of this product. Wax extracted from grain sorghum, harvested in 1996 in Nebraska, using hot hexane was fractionated with silica gel column chromatography using a series of mixtures of hexane, chloroform, methanol, and acttic acid. During TLC analysis of the sorghum wax, a dark band, which did not appear in carnauba wax, was found between was esters and TAG. This dark band fraction was the primary component, representing more than 40% of the total sorghum wax weight. The purpose of this study was to chemically characterize the dark band. The fraction containing the dark band was subjected to borohydride reduction and autoxidation by exposure to air. The borohydride reduction gave a dark band at the fatty alcohol position on TLC, whereas the oxidized sample showed a dark band at the FA position, strongly suggesting the original dark band contained aldehydes. NMR and GC-MS data confirmed that this fraction contained a saturated C28 aldehyde.

This study was performed to develop HPLC methods for determining the composition of grain sorghum wax using a silica column and an ELSD. The method development emphasized separation of aldehydes from wax esters (WE) and steryl esters (SE). Gradient mobile systems using 0.2% (v/v) acetic acid and 0.02% (v/v) methyl tert-butyl ether (MTBE) in hexane and 0.2% (v/v) acetic acid in MTBE or hexane and 0.2% (v/v) acetic acid in MTBE separated aldehydes, acids, and alcohols, as well as minor components such as mixtures of WE and SE, and triacylglycerols (TG). Grain sorghum wax was composed of 46. 3% (w/w) fatty aldehydes, 7.5% fatty acids, 41.0% fatty alcohols, 0.7% hydrocarbons, 1.4% WE and SE, and 0.9% TG, based on HPLC data. The gradient HPLC systems were also useful for compositional analysis of carnauba wax. Carnauba wax contained of 34.3% (w/w) WE, 5.1% fatty acids, undetermined amount of fatty alcohols, and 3.0% TG.

Grain sorghum wax has been judged to be a potential source of natural wax with properties similar to carnauba wax. Approximately 0.16–0.3% (w/w) wax can be extracted from grain sorghum depending on the efficiency of the organic solvents. Although the melting points of carnauba wax and sorghum wax are similar, i.e., 78–86 and 77–85°C, respectively, they differ in acid values, i.e., 2–10 and 10–16, respectively, and saponification numbers, i.e., 77–95 and 16–49, respectively. Improved knowledge of the properties, composition, and analysis of grain sorghum wax would assist in efforts for industrial application of this product. Major components of sorghum wax are hydrocarbons, wax esters, aldehydes, free fatty alcohols, and FFA. The hydrocarbons consist mainly of C27 and C29, and the aldehydes, alcohols, and acids are mainly C28 and C30. The wax esters are mostly esters of C28 and C30 alcohols and acids.

Use of near-infrared (NIR) transmittance spectroscopy for rapid determination of the oxidation level in soybean oils (SBO) was investigated, and calibrations were developed for quantitative determination of peroxide value (PV), conjugated diene value (CD), and anisidine value (AV) of SBO. Partial least squares (PLS) regression and forward stepwise multiple linear regression were used to develop calibration models from spectral data in log 1/T, first derivative and second derivative of log 1/T formats for both 1- and 2-mm path lengths. The models were validated by comparing NIR results from independent sample sets to the values obtained by official methods. The spectral region from 1100 to 2200 nm was best for measuring oxidation when using a 2-mm path length. PLS regression using first-derivative spectra gave the best results for PV. For the validation sets, linear relationships were obtained for PV (r=0.99), and CD (r=0.95), compared with accepted reference procedures. However, measurement of AV by NIR was less successful than measurement of the other two indices of oxidation, especially for an external validation sample set. Results obtained in this study indicate that NIR spectroscopy is a useful technique for measuring oxidation in soybean oil.

Edible films of starch alginate (SA), starch-alginate-stearic acid (SAS), SA-tocopherol, SAS-tocopherol, tocopherol-coated SA film, and tocopherol-coated SAS film were evaluated for their effectiveness in maintaining quality of precooked beef patties stored at 4 °C. Patty weight loss, moisture loss, 2-thiobarbituric acid-reactive substances value, the formation of hexanal, penrane, and total volatiles of samples differed with film composition. SAS-based films were more effective (P Differents types de films comestibles a base d'amidon-alginate ont ete compares, leur efficacite sur le maintien de la qualite de boulettes de viandes entreposees a 4° a ete etudie. La perte de poids, d'humidite, l'oxydation des lipides de la viande ont ete observes. Les films a base d'acide stearique sont plus efficaces dans le controle de l'humidite.Les films a base de tocopherol sont plus efficaces pour inhiber l'oxydation des lipides.

Alfalfa seeds were treated with chlorine to determine the effect on microbial populations during soak- ing, sprouting, and refrigerated storage in three packaging environments. Chlorine was effective in reducing microbial populations on the seeds, but numbers increased during sprouting. Chlorine treatments had the most impact on yeast and molds during storage. Yeast and molds were significantly higher in sprouts that were stored in vacuum packages and in sprouts from non-chlorine treated seeds stored in MAP. Yeast and mold counts on all sprouts stored in perforated packaging did not significantly increase during storage. A combination of chlorine

Two studies were conducted, using a stripped soybean oil model system, to evaluate the antioxidant interaction(s) between rosmariquinone (RQ) and oil components, including chlorophyll (CHL), β-carotene (CAR), and tocopherol(s) (TOCO), under both autoxidation and light-sensitized oxidation. In autoxidation, CHL alone had no effect on the level of oxidation, whereas CAR alone showed prooxidant (P

Nitrogen sulfur fertilizer combinations and growing season effects on sensory quality/acceptability and color of hydroponically grown greenhouse lettuce cultivar, Grand Rapids , were studied. Lettuce samples were evaluated using an attribute rating scale for appearance, color, texture, flavor, bitterness and overall acceptability, actual leaf color was measured with a chromameter. Initially, 20 N * S treatment combinations were tested. Then based on specific sensory responses, eight N * S treatment combinations were selected for further study in the Winter and in the Spring. Univariate ANOVA showed that in both studies significant (P

Battered and breaded onion rings were deep-fat fried at 170 or 190C for 30, 45, or 60s and subjected to several physical tests and to an overall desirability sensory evaluation. Hunter a color values (redness), specific shear force, and toughness increased (p < 0.01) with both frying temperature and frying time. Onion rings deep-fat fried at 190C for 60s had the highest mean overall desirability sensory score. Onion moisture content and coating redness were positively (p < 0.05) correlated with overall desirability. Coating moisture content and coating fat content were negatively (p < 0.05) correlated with overall desirability.

Gelatin-based candies were coated with medium chain triglyceride (MCT) oil or MCT oil combined with grain sorghum or carnauba wax (8.7% w/w of oil). Compared with uncoated samples, oil/sorghum wax and oil/carnauba wax coatings were equally effective in reducing protein solubility of candies in water (25C) and differences between initial and final diameters of candies subjected to melting. At 0, 5, and 10 days of storage (over calcium sulfate), the candies were evaluated by sensory panelists for surface reflection, pink color, clarity, chalkiness, firmness, adhesiveness, chewiness, cohesiveness, sweetness, sourness, fruit flavor, off-flavor, and aftertaste. Firmness, adhesiveness, chewiness, cohesiveness, and sourness scores increased (P

Rosmariquinone (1) and six analogues were chemically synthesized using an ultrasound-promoted Diels−Alder cycloaddition in yields of 35−90%. The analogues included substitution of the isopropyl at carbon 13 (C-13) with a hydrogen (5), methyl (6), or tert-butyl (4) substituent. The hydrogen-substituted analogue had the lowest yield at 35%, due in part to the instability of the compound to air, while the highest yields were achieved for the methyl (85%) and tert-butyl (90%) analogues. The 60% yield obtained for the C-14 methyl analogue (7; no C-13 isopropyl) may have been caused by the meta-substituted catechol inhibiting the cycloaddition. The final two analogues were ring A modifications and included the removal of one C-4 methyl (3; 80% yield) or both C-4 methyl (2; 85% yield) groups. The analogues were tested against rosmariquinone in light-sensitized oxidation of stripped soybean oil. Analogues 5 and 6 were significantly (P < 0.05) better antioxidants than rosmariquinone and all other analogues. The an...

Green and white asparagus were subjected to descriptive analysis, and resultant descriptors tested for effectiveness in describing and distinguishing between the two asparagus types. The effect (s) of 5 harvest times and 14 days of refrigerated storage on descriptor perception was investigated. Eleven descriptors, that described aroma (sweet potato, grassy and sulfury), flavor (sweet pea, sweet corn, bitter, and spinach) and aftertaste (buttery, brothy, meaty and grassy) of the two asparagus types, were used for the freshly harvested samples. One additional term, toughness, was added for stored product evaluation. Based on principal component analysis, it was found that green asparagus was associated with grassy and sulfury aromas; bitter and spinach flavors; and grassy aftertaste, while white asparagus was distinguished by sweet potato aroma; sweet corn flavor, buttery and brothy aftertaste, and toughness descriptors. Harvest and cold storage effects as determined by repeated measures analysis were generally asparagus type dependent and are discussed.

Glycerol-plasticized soy protein films were cast from alkaline aqueous film-forming solutions of laboratory-prepared 7S, 11S, and soy isolate (LSI) fractions and from commercial soy isolate (CSI). Tensile strength (TS), elongation at break (E), water vapor permeability (WVP), total soluble matter (TSM), protein solubility (PS), and Hunter L, a, and b color values of these films were determined. The 11S films had greater TS than 7S films (P 0.05). The 7S films had higher TSM and PS values than 11S films (P < 0.05). CSI films were significantly darker (lower L value) and more yellow (greater positive b value) than LSI films (P < 0.05).

Normal corn starch with 15 to 21% (db) moisture content was extrusion-cooked in a twin-screw extruder. Cinnamaldehyde, nonanoic acid, 3-octanone and eugenol, at concentrations of 1 to 5% (w/w), were either premixed with the feed or injected into the barrel. Response surface methodology was used to predict percent initial amount retained. Radial expansion ratios were different when volatile components were premixed with the feed for all components except 3-octanone. Only nonanoic acid influenced (P > F = 0.001) specific mechanical energy values due to the method of addition. Volatile component retention was enhanced when they were injected into the extruder barrel as compared to pre-mixing with the feed.

Methyl tallowate was prepared from edible beef tallow via transesterification, and was blended with ethanol and/or No.2 diesel fuel in different ratios. Crystallization characteristics of methyl tallowate and its blends were studied at temperatures ranging from 22 to −16°C. Blending ethanol with methyl tallowate reduced crystal formation at all temperatures. As the temperature of the blends was reduced from 22 to 0°C, there was no effect on crystal formation of saturated vs. unsaturated fatty acids. Below 0°C, the saturated fatty acids crystallized at a much faster rate than the unsaturated fatty acids.

Fuel properties of beef tallow, soybean oil, their esters, and blends with No. 2 diesel fuel and ethanol were determined. Fuel properties tested were viscosity, specific gravity, API gravity, distillation ranges, calculated cetane index, energy content, flash point, water content, sulfur content, carbon residue, particulate matter, acid value, copper-strip corrosion test, ash content, melting point, cloud point, and pour point. Gas-chromatographic analyses of tallow, soybean oil, and their esters were performed to determine their major constituents. Viscosities of soybean oil and tallow were significantly reduced by esterification. Other fuel properties of the esters and their blends with No. 2 diesel fuel and ethanol were similar to the properties of No. 2 diesel fuel.

The objective of this study was to evaluate various properties of low browning Mozzarella cheese. Low moisture, part skim type Mozzarella cheese was made using strains of Streptococcus spp. and Lactobacillus helveticus that release inappreciable amounts of galactose into the cheese curd and was compared with high browning cheese made using cultures that release galactose into the curd. Cheese composition differed only for galactose content. The browning potentials of the cheeses were significantly different. Pizzas were made using 125g of each cheese type and evaluated for appearance, texture, and flavor by a consumer panel. Panelists could distinguish between pizza samples based on color of the baked pizza, although no differences or preferences based on any of the other criteria were discernible. Both brown and low browned pizza were acceptable by the panelists, indicating that browning of Mozzarella cheese on pizza was not an undesirable property.

Supercritical carbon dioxide (SC-CO2) extraction was studied for removal of lipids and cholesterol from dried chicken meat powder and chunks. Two combinations of pressure and temperature were used: 299 atm and 45 C, and 381 atm and 55 C, both providing a fluid density of .90 g/cm3. For a given quantity of CO2, at the higher temperature and pressure, significantly (P < .05) more lipids and cholesterol were extracted from the powder. At 381 atm and 55 C, approximately 89% of the lipids and 90% of the cholesterol were removed from the dehydrated chicken meat powder. With respect to the chunk chicken meat, about 93% of the lipids and 82% of the cholesterol were extracted at 299 atm and 45 C. It seemed that the SC-CO2 extraction process was more efficient when chunks were used. Protein was concentrated as cholesterol and lipids were removed by SC-CO2 extraction of both chicken meat types, and Hunterlab L values increased but aL values decreased, indicating a lighter color with less redness. This research indicated that SC-CO2 extraction holds promise for substantially reducing lipids and cholesterol in chicken meat.

Supercritical carbon dioride (SC-CO 2 ) was used to extract cholesterol and other lipids from dehydrated beef powders and chunks. When powders were extracted at 55 o C and a fluid density of 0.90 g/cm 3 , the cholesterol and total fat contents of the product could both be reduced by as much as 87%. Lipids were more easily extracted from chunks than from powders, allowing for successful removal at lower temperatures and pressures

Supercritical carbon dioxide extraction was investigated for removal of cholesterol and lipid components from dried egg yolk. Four different combinations of pressure and temperature were used including 163 atm/40°C, 238 atm/45°C, 306 atm/45°C and 374 atm/55°C. As temperature and pressure were increased, more lipids and cholesterol were removed. Extraction at 306 atm/45°C or 374 atm/55°C removed approximately two-thirds of the cholesterol. Phospholipids and protein were concentrated under the supercritical extraction conditions used. Sponge cake volume was significantly (P < 0.05) improved at extraction conditions of 163 atm/40°C, 238 atm/45°C and 306 atm/45°C, which may have been due to higher phospholipid and protein concentrations. Only extraction at 374 atm/55°C significantly (P < 0.05) impaired emulsion stability of egg yolk.

Evidence indicates that oxidative stress inhibits cell proliferation in several cell systems. To determine whether the proliferation of Caco-2 cells is inhibited by oxidative stress and to identify any novel key regulatory factors involved in protecting or damaging the intestine from oxidative stress, Caco-2 cells were treated with an oxidizing agent and analyzed by transcriptomic oligonucleotide microarrays. Results indicated that expression of genes involved in cell proliferation and growth, including genes involved in lipid synthesis, cell cycle progression and cell division, angiogenesis, RNA processing and translation, cAMP metabolism, cytoskeleton and cell to cell adhesion, receptor tyrosine kinases, and intracellular and extracellular signaling, were repressed. If an oxidant-induced inhibition in cell proliferation is involved in the pathogenesis of intestinal disease, information gained could help explain the mechanisms contributing to the causes and consequences of intestinal disease and could aid in the elucidation of mechanisms by which intestinal cells protect against oxidative stress.

The effects of 24 h supplementation of Caco-2 cells with carnosic acid and carnosol, and their activities against 5 μM oleic acid hydroperoxide (OAHPx)-mediated oxidative stress, were investigated. At 24 h of incubation, under nonstressed and stressed conditions, both compounds at 25, 50, and 100 μM supplement concentrations reduced catalase activity, whereas changes in glutathione peroxidase and superoxide dismutase activities varied depending upon the concentrations. Relative to control cultures, carnosic acid and carnosol reduced membrane damage by 40−50% when stressed by OAHPx. Carnosic acid and carnosol inhibited lipid peroxidation by 88−100% and 38−89%, respectively, under oxidative stress conditions. Both compounds significantly lowered DNA damage induced by OAHPx. Results of this study suggest that antioxidant activities of carnosic acid and carnosol could be partly due to their ability to increase or maintain glutathione peroxidase and superoxide dismutase activities. Keywords: Oxidative stress; ...

Experiments were carried out on a lab supercritical CO(2) extraction system to determine the effects of extraction conditions, including mass ratio of CO(2) consumed to distillers dry grain with solubles (DDGS) extracted, extraction pressure, extraction temperature and time, on yield and composition of extracted lipids. A maximum lipid yield of 150 g/kg DDGS was achieved with a mass ratio approximately 45, an extraction pressure at 27.5 MPa, an extraction temperature at 70 degrees C and an extraction time of 4 h. Under these extraction conditions, the contents of tocols, phytosterols, policosanols and free fatty acids were 0.44, 15.6, 31.2 and 155.3 mg/g in the extract. Experimental results indicated that shorter extraction time and higher flow rate of CO(2) can achieve higher contents of tocols, phytosterols and policosanols but lower content of free fatty acids in the lipid extract. Extraction conditions had no observed effects on the composition of free fatty acids in the extract. Palmitic, oleic and linoleic acids were three main free fatty acids extracted and constituted about 94% of all free fatty acids.

Grain sorghum is a rich source of phytochemicals that could potentially benefit human health. In this study, male hamsters were fed AIN-93M diets supplemented with a hexane-extractable lipid fraction from grain sorghum whole kernels. The grain sorghum lipids (GSL) comprised 0.0, 0.5, 1.0, or 5.0% of the diet by weight. After 4 wk, dietary GSL significantly reduced plasma non-HDL cholesterol concentration in a dose-dependent manner with reductions of 18, 36, and 69% in hamsters fed 0.5, 1.0, and 5.0% GSL, respectively, compared with controls. Liver cholesteryl ester concentration was also significantly reduced in hamsters fed GSL. Plasma HDL cholesterol concentration was not altered (P > 0.05) by dietary treatment. Cholesterol absorption efficiency was significantly reduced by GSL in a dose-dependent manner. Cholesterol absorption was also directly correlated with plasma non-HDL cholesterol concentration (r = 0.97, P < 0.05), suggesting that dietary GSL lowers non-HDL cholesterol, at least in part, by inhibiting cholesterol absorption. TLC and GLC analyses of the GSL extract revealed the presence of plant sterols and policosanols at concentrations of 0.35 and 8.0 g/100 g GSL, respectively. Although plant sterols reduce cholesterol absorption, policosanols may inhibit endogenous cholesterol synthesis. The data suggest that these components of GSL extract may work collectively in lowering plasma and liver cholesterol concentrations. Our findings further indicate that grain sorghum contains beneficial components that could be used as food ingredients or dietary supplements to manage cholesterol levels in humans.

Synthetic rosmariquinone (RQ), an antioxidant naturally present in rosemary (Rosmarinus officinalis L.), tertiary butylhydroquinone (TBHQ) and rosemary oleoresin (bleached and unbleached), was tested for antioxidant activity in stripped and nonstripped soybean oil in a light-induced oxidation system. In the stripped soybean oil system, RQ was significantly less (P

Combining the advantages of polysaccharides, proteins and/or lipids offers multicomponent edible films and coatings good mass transfer barrier properties. Multicomponent edible films and coatings could be beneficial to the food industry by leading to innovative applications. The barrier properties of these systems strongly depend upon their structure and chemistry, the interaction between different film components as well as surrounding environment conditions. Future researches on these multicomponent systems need to concentrate on the following goals: (1) investigation of the optimal film compositions and specific film-forming conditions for different food systems; (2) study of the film responses in their barrier properties to environmental factors such as RH and temperature; (3) fundamental research of the mechanism of mass transfer and the interaction of different films components and foods; (4) exploration of the feasibility of potential applications in the food industry.

Combining the advantages of polysaccharides, proteins and/or lipids offers multicomponent edible films and coatings good mass transfer barrier properties. Multicomponent edible films and coatings could be beneficial to the food industry by leading to innovative applications. The barrier properties of these systems strongly depend upon their structure and chemistry, the interaction between different film components as well as surrounding environment conditions. Future researches on these multi- component systems need to concentrate on the following goals: (1) investigation of the optimal film compositions and specific film-forming conditions for different food systems; (2) study of the film responses in their barrier properties to environmental factors such as RH and temperature; (3) fundamental research of the mechanism of mass transfer and the interaction of different films components and foods; (4) exploration of the feasibility of potential applications in the food industry.