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There is a need for information on pollen exposure to assess allergy risk. Monitoring of aeroallergens in a city is usually limited to the use of a single trap for the whole area. While a single trap provides enough information on background pollen concentration for the area, varying pollen exposure across different urban environments, however, is not considered. In this study, we analysed aerobiological data of three pollen seasons (2017–2020) recorded with a volumetric pollen trap in Sydney, Australia. In order to assess spatial differences in pollen exposure across the city, we installed ten gravimetric traps recording pollen deposition for eight weeks during the summer of 2019/2020. We considered the influence of meteorological variables, land use, urbanisation and distance to the sea. Our results showed differences in pollen season characteristics across the three analysed seasons and correlations with meteorological parameters. Considering all years, we found for Poaceae and Alternaria the strongest positive correlation with mean temperature and for Myrtaceae and Cupressaceae with maximum temperature. Likewise, there were negative correlations with humidity (Myrtaceae, Cupressaceae, Alternaria) and precipitation (Myrtaceae, Cupressaceae). Days with medically relevant pollen and spore concentrations varied between years and we recorded the highest amount in 2017/2018 for Poaceae and Alternaria and in 2019/2020 for Myrtaceae. In addition, we found spatial and temporal variations of pollen deposition. However, we did not detect significant correlations between pollen deposition and land use, which can be attributable to drought conditions prior to the sampling campaign and the temporal setting in the pollen season. This study highlights the importance of continuous volumetric aerobiological monitoring as well as the assessment of pollen exposure at several locations across a large urban area.

Viruses are frequently a microbial biocontaminant of healthy plants. The occurrence of the infection can be also due to environmental stress, like urbanisation, air pollution and increased air temperature, especially under the ongoing climate change. The aim of the present study was to investigate the hypothesis that worsened air quality and fewer green areas may favour the higher frequency of common viral infections, particularly in a common tree in temperate and continental climates, Betula pendula ROTH. We examined 18 trees, during the years 2015–2017, the same always for each year, in the region of Augsburg, Germany. By specific PCR, the frequency of two viruses, Cherry leaf roll virus (CLRV, genus Nepovirus, family Secoviridae), which is frequent in birch trees, and a novel virus tentatively named birch idaeovirus (BIV), which has been only recently described, were determined in pollen samples. The occurrence of the viruses was examined against the variables of urban index, air pollution (O3 and NO2), air temperature, and tree morphometrics (trunk perimeter, tree height, crown height and diameter). Generalized Non-linear models (binomial logit with backward stepwise removal of independent variables) were employed. During the study period, both CLRV and BIV were distributed widely throughout the investigated birch individuals. CLRV seemed to be rather cosmopolitan and was present independent of any abiotic factor. BIV's occurrence was mostly determined by higher values of the urban index and of NO2. Urban birch trees, located next to high-traffic roads with higher NO2 levels, are more likely to be infected by BIV. Increased environmental stress may lead to more plant viral infections. Here we suggest that this is particularly true for urban spaces, near high-traffic roads, where plants may be more stressed, and we recommend taking mitigation measures for controlling negative human interventions.

Standard pollen monitoring programs evaluate outdoor pollen concentrations; however, information on indoor pollen is crucial for human wellbeing as people spend most of the day in indoor environments. In this study, we investigated the differences in indoor mountain cedar pollen loads between rooms of different uses and with different ventilation at The University of Texas in Austin and focused on the effect of rainy episodes on indoor/outdoor ratios of pollen concentrations. Pollen were sampled outdoors and indoors, specifically in seven rooms and in two thermal labs with controlled ventilation, during the daytime on 6 days in 2015. We calculated daily pollen concentrations, campaign pollen integrals (CPIn, the sum of all daily pollen concentrations) and ratios between indoor and outdoor concentrations (I/O ratio). Pollen concentrations differed substantially based on features related to room use and ventilation: Whereas the highest CPIn was observed in a room characterized by a frequently opened window and door, the smallest CPIn was related to a storeroom without any windows and no forced ventilation. Our results showed that rainy episodes were linked to a higher mean I/O ratio (0.98; non-rainy episodes: 0.05). This suggests that pollen accumulated indoors and reached higher levels than outdoors. Low ratios seem to signal a low level of risk for allergic people when staying inside. However, under very high outdoor pollen concentrations, small ratios can still be associated with high indoor pollen levels. In turn, high I/O ratios are not necessarily related to a (very) high indoor exposure. Therefore, I/O ratios should be considered along with pollen concentration values for a proper risk assessment. Exposure may be higher in indoor environments during prevailing precipitation events and at the end of the pollen season of a specific species. Standardized indoor environments (e.g., thermal labs) should be included in pollen monitoring programs.

Intraspecific genetic variation might limit the relevance of environmental factors on plant traits. For example, the interaction between genetics and (a-)biotic factors regulating pollen production are still poorly understood. In this study, we investigated pollen production of 28 birch (Betula pendula Roth) individuals in the years 2019–2021. We sampled catkins of eleven groups of genetically identical trees, which were partially topped, but of the same age and located in a seed plantation in southern Germany characterized by similar microclimatic conditions. Furthermore, we monitored environmental factors such as air temperature, characterized air quality (NO2, NOx and O3), and assessed potential solar radiation. We especially checked for differences between years as well as between and within clones and assessed the synchronicity of years with high/low pollen production. We present a robust mean for the pollen production of Betula pendula (1.66 million pollen grains per catkin). Our findings show temporal (H(2) = 46.29, p < 0.001) and clonal variations (H(4) = 21.44, p < 0.001) in pollen production. We conclude that synchronized high or low pollen production is not utterly site-specific and, in addition, not strictly dependent on genotypes. We suggest that appropriate clone selection based on application (seed plantation, urban planting) might be advantageous and encourage a long-term monitoring. German Research Foundation (Deutsche Forschungsgemeinschaft) (DFG) Open Access Fund of the Catholic University Eichstätt-Ingolstadt EU-COST Action ADOPT

Standard pollen monitoring programs evaluate outdoor pollen concentrations; however, information on indoor pollen is crucial for human wellbeing as people spend most of the day in indoor environments. In this study, we investigated the differences in indoor mountain cedar pollen loads between rooms of different uses and with different ventilation at The University of Texas in Austin and focused on the effect of rainy episodes on indoor/outdoor ratios of pollen concentrations. Pollen were sampled outdoors and indoors, specifically in seven rooms and in two thermal labs with controlled ventilation, during the daytime on 6 days in 2015. We calculated daily pollen concentrations, campaign pollen integrals (CPIn, the sum of all daily pollen concentrations) and ratios between indoor and outdoor concentrations (I/O ratio). Pollen concentrations differed substantially based on features related to room use and ventilation: Whereas the highest CPIn was observed in a room characterized by a frequently opened window and door, the smallest CPIn was related to a storeroom without any windows and no forced ventilation. Our results showed that rainy episodes were linked to a higher mean I/O ratio (0.98; non-rainy episodes: 0.05). This suggests that pollen accumulated indoors and reached higher levels than outdoors. Low ratios seem to signal a low level of risk for allergic people when staying inside. However, under very high outdoor pollen concentrations, small ratios can still be associated with high indoor pollen levels. In turn, high I/O ratios are not necessarily related to a (very) high indoor exposure. Therefore, I/O ratios should be considered along with pollen concentration values for a proper risk assessment. Exposure may be higher in indoor environments during prevailing precipitation events and at the end of the pollen season of a specific species. Standardized indoor environments (e.g., thermal labs) should be included in pollen monitoring programs.

Standard pollen monitoring programs evaluate outdoor pollen concentrations; however, information on indoor pollen is crucial for human wellbeing as people spend most of the day in indoor environments. In this study, we investigated the differences in indoor mountain cedar pollen loads between rooms of different uses and with different ventilation at The University of Texas in Austin and focused on the effect of rainy episodes on indoor/outdoor ratios of pollen concentrations. Pollen were sampled outdoors and indoors, specifically in seven rooms and in two thermal labs with controlled ventilation, during the daytime on 6 days in 2015. We calculated daily pollen concentrations, campaign pollen integrals (CPIn, the sum of all daily pollen concentrations) and ratios between indoor and outdoor concentrations (I/O ratio). Pollen concentrations differed substantially based on features related to room use and ventilation: Whereas the highest CPIn was observed in a room characterized by a frequently opened window and door, the smallest CPIn was related to a storeroom without any windows and no forced ventilation. Our results showed that rainy episodes were linked to a higher mean I/O ratio (0.98; non-rainy episodes: 0.05). This suggests that pollen accumulated indoors and reached higher levels than outdoors. Low ratios seem to signal a low level of risk for allergic people when staying inside. However, under very high outdoor pollen concentrations, small ratios can still be associated with high indoor pollen levels. In turn, high I/O ratios are not necessarily related to a (very) high indoor exposure. Therefore, I/O ratios should be considered along with pollen concentration values for a proper risk assessment. Exposure may be higher in indoor environments during prevailing precipitation events and at the end of the pollen season of a specific species. Standardized indoor environments (e.g., thermal labs) should be included in pollen monitoring programs.

The prevalence of pollen allergy is increasing worldwide, as is the proportion of people living in cities. Thus, there is an increasing importance to investigate pollen distribution across city districts. We conducted two sampling campaigns to investigate the spatial and temporal variation of airborne pollen in the Sydney metropolitan area and the vertical variation within a forest in north-western Sydney.Spatial assessment of pollen deposition was made for eight weeks in the exceptionally dry summer in 2019/2020 using gravimetric samplers. These samplers were set up at ten locations characterised by different degrees of urbanisation and distance to the sea. We focussed on the most abundant pollen types and investigated statistical relationships with land use and meteorology. In addition, we compared our results with pollen data of previous years sampled at a pollen monitoring station located in north-western Sydney in a semi-rural environment. We measured vertical pollen concentrations in a native forest, which mostly consists of Eucalypt trees (family Myrtaceae) in north-western Sydney. A scaffolding was equipped with five portable volumetric pollen samplers installed at different heights (1, 4, 10, 16, 20.5 m above ground level (agl)). We measured pollen concentration every second hour between 9 am and 4 pm on a total of four days in January 2020. We compared concentrations between days, heights, and times of the day.The most abundant pollen type registered within our sampling campaigns belonged to the family Myrtaceae. Grass pollen (Poaceae) was also detected, but in much smaller quantities which can be attributed to the drought and temporal setting of the campaign, which started in the post-peak period of a comparably weak pollen season associated with a smaller number of days with medical relevance (> 50 pollen grains/m3). Our data showed spatial variations between the ten locations, but no relationship with land use (grass and tree cover) and meteorology could be found. This suggests the influence of other factors such as long-range pollen transport or resuspension of pollen. In the forest, Myrtaceae concentrations varied between days, sampling height and time of the day: the highest concentration was recorded on the second day of measurement between 9 and 10 am at 10 m agl. Peak values were generally reached between 1 and 2 pm. Considering sampling height, concentrations were on average highest at 4 m agl. The location of pollen sources as well as meteorological conditions such as turbulence and variation in wind speed may be key determinants of small-scale differences of pollen concentrations.The drought preceding this study did not only influence the length but also the strength of the pollen season. Data on vertical variations could support investigations related to turbulence, which is also responsible for resuspension processes.

It has been argued in the literature that global temperature increased at a reduced rate between approximately 1998 and 2013, a phenomenon known as the ‘global warming hiatus’. Statistically motivated studies searching for numerical evidence for this episode typically argue against the detectability of a slowdown. At the same time, process-oriented studies on potential causes of the slowdown are more focused on establishing the consistency between observations and model-based expectations of surface warming. Here, we employ three different gridded temperature data sets, two different statistical tests, and four different sets of periods for defining a baseline period and slowdown period associated to the recent slowdown in surface warming to provide strong evidence against consistent regional patterns of significant changes in the warming trend. Roughly half of the earth’s surface has experienced a reduced warming trend during the slowdown period, which is strongly connected to the Interdecadal Pacific Oscillation as a main source of internal variability of the climate system. This finding is consistent with the understanding of the role of internal decadal variability of the climate obtained from modelling and attribution studies. However, controlling for false discovery rates in the context of multiple testing in the spatial domain, we found that only less than 5% of the earth’s surface have experienced a significant regional slowdown in surface warming. At the same time, for roughly the same small number of grid cells we found the opposite pattern of a significant acceleration in warming. In both cases, the identified areas are not robust against the choice of temperature data set and statistical test, as well as against the delineation of the testing periods for baseline and slowdown periods. Our results demonstrate that similar to previous findings at the global level, statistical testing accounting for the multiple testing nature of the problem argues against a robust detectability of the ‘warming hiatus’ at the regional level.

Austin, Texas, is one of the worst places with respect to allergies. In winter, mountain cedar ( Juniperus ashei ) is producing high pollen amounts exerting the so-called cedar fever in individuals allergic to this pollen species. In this study, we evaluated to which extent the effect of prescribed fires in a semi-arid ecosystem decreases pollen concentrations at the local scale. The study was performed on patches characterized by human-induced fire disturbances and unmanaged areas (control sites) in the southwest of Austin. Pollen was sampled on four consecutive days in January 2015 using ten pollen traps installed at 1.5 m a.g.l. Microscope slides were inserted every second hour during 8 am and 6 pm. Each station was equipped with meteorological devices to account for influencing factors such as wind speed and direction. Mean cedar pollen concentrations differed across the studied days and plots and ranged between 633 (16th January) and 126,400 pollen grains/m 3 (17th January). We determined diurnal patterns with peak values between 2 and 3 pm. Pollen concentrations were on average 15% lower at burnt vs. control plots. Comparing pairs of adjacent plots even revealed a reduction of up to 50%. Therefore, local land management can alter pollen concentrations drastically. A walk across previously burnt areas may trigger less severe symptoms for allergic people during the flowering period of mountain cedar. Our results add to the knowledge about individual cedar pollen exposure in heterogeneous areas and help improving mitigation strategies.