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22 results on '"Suschak, John J."'

7. The role of vaccination route with an adenovirus-vectored vaccine in protection, viral control, and transmission in the SARS-CoV-2/K18-hACE2 mouse infection model

Author

Dickson, Alexandria, primary, Geerling, Elizabeth, additional, Stone, E. Taylor, additional, Hassert, Mariah, additional, Steffen, Tara L., additional, Makkena, Taneesh, additional, Smither, Madeleine, additional, Schwetye, Katherine E., additional, Zhang, Jianfeng, additional, Georges, Bertrand, additional, Roberts, M. Scot, additional, Suschak, John J., additional, Pinto, Amelia K., additional, and Brien, James D., additional

Published
2023
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8. A single intranasal administration of AdCOVID protects against SARS-CoV-2 infection in the upper and lower respiratory tracts

Author

Schultz, Michael D., primary, Suschak, John J., additional, Botta, Davide, additional, Silva-Sanchez, Aaron, additional, King, R. Glenn, additional, Detchemendy, Thomas W., additional, Meshram, Chetan D., additional, Foote, Jeremy B., additional, Zhou, Fen, additional, Tipper, Jennifer L., additional, Zhang, Jianfeng, additional, Harrod, Kevin S., additional, Leal, Sixto M., additional, Randall, Troy D., additional, Roberts, M. Scot, additional, Georges, Bertrand, additional, and Lund, Frances E., additional

Published
2022
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10. Single-Dose Intranasal Administration of AdCOVID Elicits Systemic and Mucosal Immunity against SARS-CoV-2 and Fully Protects Mice from Lethal Challenge

Author

King, R. Glenn, primary, Silva-Sanchez, Aaron, additional, Peel, Jessica N., additional, Botta, Davide, additional, Dickson, Alexandria M., additional, Pinto, Amelia K., additional, Meza-Perez, Selene, additional, Allie, S. Rameeza, additional, Schultz, Michael D., additional, Liu, Mingyong, additional, Bradley, John E., additional, Qiu, Shihong, additional, Yang, Guang, additional, Zhou, Fen, additional, Zumaquero, Esther, additional, Simpler, Thomas S., additional, Mousseau, Betty, additional, Killian, John T., additional, Dean, Brittany, additional, Shang, Qiao, additional, Tipper, Jennifer L., additional, Risley, Christopher A., additional, Harrod, Kevin S., additional, Feng, Tsungwei, additional, Lee, Young, additional, Shiberu, Bethlehem, additional, Krishnan, Vyjayanthi, additional, Peguillet, Isabelle, additional, Zhang, Jianfeng, additional, Green, Todd J., additional, Randall, Troy D., additional, Suschak, John J., additional, Georges, Bertrand, additional, Brien, James D., additional, Lund, Frances E., additional, and Roberts, M. Scot, additional

Published
2021
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14. A DNA vaccine for Crimean-Congo hemorrhagic fever protects against disease and death in two lethal mouse models

Author

Garrison, Aura R., primary, Shoemaker, Charles J., additional, Golden, Joseph W., additional, Fitzpatrick, Collin J., additional, Suschak, John J., additional, Richards, Michelle J., additional, Badger, Catherine V., additional, Six, Carolyn M., additional, Martin, Jacqueline D., additional, Hannaman, Drew, additional, Zivcec, Marko, additional, Bergeron, Eric, additional, Koehler, Jeffrey W., additional, and Schmaljohn, Connie S., additional

Published
2017
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16. An immunoinformatics-derived DNA vaccine encoding human class II T cell epitopes of Ebola virus, Sudan virus, and Venezuelan equine encephalitis virus is immunogenic in HLA transgenic mice

Author

Bounds, Callie E., primary, Terry, Frances E., additional, Moise, Leonard, additional, Hannaman, Drew, additional, Martin, William D., additional, De Groot, Anne S., additional, Suschak, John J., additional, Dupuy, Lesley C., additional, and Schmaljohn, Connie S., additional

Published
2017
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17. Identification of Aim2 as a sensor for DNA vaccines 1

Author

Suschak, John J., Wang, Shixia, Fitzgerald, Katherine A., and Lu, Shan

Subjects
DNA-Binding Proteins, Mice, Knockout, Mice, Inflammasomes, Interleukin-1beta, Interleukin-18, Vaccines, DNA, Animals, Humans, Interferon-alpha, Interferon-beta, Article, Immunity, Innate
Abstract

Recent human study data have re-established the value of DNA vaccines, especially in priming high-level Ag-specific Ab responses, but also raised questions about the mechanisms responsible for such effects. Whereas previous reports have shown involvement of downstream signaling molecules in the innate immune system, the current study investigated the role of absent in melanoma 2 (Aim2) as a sensor for DNA vaccines. The Aim2 inflammasome directs maturation of the proinflammatory cytokines IL-1β and IL-18 and an inflammatory form of cell death called pyroptosis. Both the humoral and cellular Ag-specific adaptive responses were significantly reduced in Aim2-deficient mice in an IL-1β/IL-18-independent manner after DNA vaccination. Surprisingly, Aim2-deficient mice also exhibited significantly lower levels of IFN-α/β at the site of injection. These results indicate a previously unreported link between DNA vaccine-induced pyroptotic cell death and vaccine immunogenicity that is instrumental in shaping the Ag-specific immune response to DNA vaccines.

Published
2014

21. Nanoplasmid Vectors Co-expressing Innate Immune Agonists Enhance DNA Vaccines for Venezuelan Equine Encephalitis Virus and Ebola Virus.

Author

Suschak JJ, Dupuy LC, Shoemaker CJ, Six C, Kwilas SA, Spik KW, Williams JA, and Schmaljohn CS

Abstract

DNA vaccines expressing codon-optimized Venezuelan equine encephalitis virus (VEEV) and Ebola virus (EBOV) glycoprotein genes provide protective immunity to mice and nonhuman primates when delivered by intramuscular (IM) electroporation (EP). To achieve equivalent protective efficacy in the absence of EP, we evaluated VEEV and EBOV DNA vaccines constructed using minimalized Nanoplasmid expression vectors that are smaller than conventional plasmids used for DNA vaccination. These vectors may also be designed to co-express type I interferon inducing innate immune agonist genes that have an adjuvant effect. Nanoplasmid vaccinated mice had increased antibody responses as compared to those receiving our conventional pWRG7077-based vaccines when delivered by IM injection, and these responses were further enhanced by the inclusion of the innate immune agonist genes. The Nanoplasmid VEEV DNA vaccines also significantly increased protection against aerosol VEEV challenge as compared to the pWRG7077 VEEV DNA vaccine. Although all mice receiving the pWRG7077 and Nanoplasmid EBOV DNA vaccines at the dose tested survived EBOV challenge, only mice receiving the Nanoplasmid EBOV DNA vaccine that co-expresses the innate immune agonist genes failed to lose weight after challenge. Our results suggest that Nanoplasmid vectors can improve the immunogenicity and protective efficacy of alphavirus and filovirus DNA vaccines., (© 2020 The Authors.)

Published
2020
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22. Future Approaches to DNA Vaccination Against Hemorrhagic Fever Viruses.

Author

Suschak JJ and Schmaljohn CS

Subjects
Adjuvants, Immunologic, Animals, Electroporation, Hemorrhagic Fever, Ebola immunology, Humans, Vaccines, DNA immunology, Hemorrhagic Fever, Ebola prevention & control, Vaccines, DNA therapeutic use
Abstract

To date, there is no protective vaccine for Ebola virus infection. Safety concerns have prevented the use of live-attenuated vaccines, and forced researchers to examine new vaccine formulations. DNA vaccination is an attractive method for inducing protective immunity to a variety of pathogens, but the low immunogenicity seen in larger animals and humans has hindered its usage. Various approaches have been used to improve the immunogenicity of DNA vaccines, but the most successful, and widespread, is electroporation. Of increasing interest is the use of molecular adjuvants to produce immunomodulatory signals that can both amplify and direct the immune response. When combined, these approaches have the possibility to push DNA vaccination into the forefront of medicine.

Published
2018
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