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173 results on '"Svinarov, Dobrin"'

1. Evaluation of routine laboratory markers as short-term outcome indicators in patients with acute ischemic stroke

Author

Popovska Tatyana, Kalpachki Rosen, Svinarov Dobrin, and Velizarova Milena

Subjects
crp, d-dimer, fibrinogen, ischemic stroke, laboratory markers, Medicine
Abstract

Introduction: Stroke is a disease with high mortality and morbidity. Currently, there are no specific laboratory markers that can replace or improve clinical and radiological diagnosis and prognosis. We evaluated the role of C-reactive protein (CRP), fibrinogen and D-dimer in predicting short-term outcomes in acute ischemic stroke.

Published
2024
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2. Development and validation of an LC-MS/MS method for determination of 8-iso-prostaglandin f2 Alpha in human saliva

Author

Tomova Zlatina, Tomov Desislav, Vlahova Angelina, Chaova-Gizdakova Veneta, Yoanidu Lyubka, and Svinarov Dobrin

Subjects
metal alloy, 8-isopgf2a, lc-ms/ms, saliva, salle, Biochemistry, QD415-436
Abstract

Background: Increased formation of reactive oxygen species may be caused by the ion release of the metal alloys used in prosthetic dental restorations due to the corrosion process. As products of lipid peroxidation, isoprostanes can be used as a marker for oxidative stress in the body. There are two significant advantages of using isoprostanes as an oxidative stress marker - presence in all fluids in the body and low reactivity. Saliva provides noninvasive, painless, and cost-effective sample collection and can be used as an alternative testing medium of blood and urine. Methods: This study presents the development and validation of a sample LC-MS/MS method to quantify 8-isoprostaglandin F2-a in human saliva using salt-out assisted liquid-liquid extraction (SALLE). Results: The selected sample preparation procedure optimized chromatographic separation and mass detection provided high recovery and sensitivity of the analysis. The calibration curve was obtained in the predefined range 25-329 ng/L with R2 larger than 0.995. Normalized matrix varied between 89.7 % and 113.5%. The method showed sufficient accuracy and precision - accuracy in the range 89.7 %-113.9 %, and precision between 2.3% and 5.4%. Conclusions: The proposed method is validated according to current EMA/FDA industrial guidance for bioanalysis and offers an appropriate level of sensitivity and sufficient accuracy and precision.

Published
2022
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6. Phase separation liquid-liquid extraction for the quantification of 8-iso-Prostaglandin F2 Alpha in human plasma by LC-MS/MS

Author

Tomov Desislav G., Bocheva Georgeta, Divarova Vidka, Kasabova Lilia, and Svinarov Dobrin

Subjects
8-iso-prostaglandin f2a, liquid extraction with phase separation, lc-ms/ms, Biochemistry, QD415-436
Abstract

Background: Reactive oxygen species (ROS) are produced in the body during normal metabolism by means of enzymes and non-enzymatic chemical reduction of molecular oxygen. In case of the prevalence of ROS formation over their elimination, highly reactive free radicals can be accumulated and can cause multiple damages to the biomolecules and cells. Determination of isoprostanes in biological matrices is most often used to register free radical damage and requires selective, sensitive and specific techniques. Methods: This study presents the development and validation of the LC-MS/MS method for the determination of 8-iso-Prostaglandin F2a in human plasma utilising a modified liquid-liquid extraction procedure with phase separation. Results: Modified sample preparation procedure assured higher extraction yield, clear separation of organic layer from the plasma water phase and protein precipitates, and better-purified product for instrumental analysis. Linearity was validated in the range 0.1-5.0 mg/L with R2 > 0.996; normalised matrix varied between 86.0% and 108.3%, accuracy ranged from 90.4 % to 113.9% and precision both within runs and between runs was less than 7%. With a run time of 10 min, a throughput of over 50 samples per working day could be performed. Conclusions: The method meets all the current industrial validation criteria and allows the accurate and precise determination of 8-iso-PGF2a in human plasma at diagnostically significant concentration range.

Published
2021
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13. IL-6 and SAA—Strong Predictors for the Outcome in COVID-19 CKD Patients.

Author

Filev, Rumen, Lyubomirova, Mila, Bogov, Boris, Kalinov, Krassimir, Hristova, Julieta, Svinarov, Dobrin, and Rostaing, Lionel

Subjects
COVID-19, INTERLEUKIN-6, COVID-19 pandemic, ACUTE kidney failure, PATHOLOGICAL physiology
Abstract

In this prospective study, we assessed biomarkers of inflammation (IL-6 and SAA) from the serum of 120 COVID-19 patients, of whom 70 had chronic kidney disease. All the samples were taken at emergency-department (ED) admission. Our goal was to relate the biomarkers to the results of death and acute kidney injury. All the patients underwent chest computer tomography to estimate the severity score (0–5), which was performed at hospital admission. Finally, biomarkers were also evaluated in a healthy control group and in non-COVID-19-CKD patients. IL-6 and SAA were statistically different between the subgroups, i.e., they were significantly increased in patients with COVID-19. Both of the biomarkers (IL-6 and SAA) were independently associated with mortality, AKI and a higher grade of pathological changes in the lung's parenchyma. Both high baseline levels of IL-6 and SAA on hospital admission were highly correlated with a later ventilatory requirement and mortality, independent of hospital stay. Mortality was found to be significantly higher when the chest CT severity score was 3–4, compared with a severity score of 0–2 (p < 0.0001). Conclusions: at the admission stage, IL-6 and SAA are useful markers for COVID-19 patients with CKD. [ABSTRACT FROM AUTHOR]

Published
2024
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17. Diagnostic Significance of Cholangiocarcinoma-associated Carbohydrate Antigen (CCA-CA) and Insulin-like Growth Factor 1 (IGF-1) in Blood Serum and Bile in Patients with Extrahepatic Bile Duct Cancer and Obstructive Jaundice

Author

Stoyanov, Vladislav, primary, Dardanov, Dragomir, additional, Arabadzhieva, Elena, additional, Yonkov, Atanas, additional, Bonev, Sasho, additional, Bulanov, Dimitar, additional, Hristova, Julieta, additional, Simonova, Lilia, additional, Dimitrova, Violeta, additional, and Svinarov, Dobrin, additional

Published
2019
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23. Editorial

Author

Svinarov, Dobrin, primary, Paskaleva, Ivanka, additional, and Velizarova, Milena, additional

Published
2022
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28. A proposed Common Training Framework for Specialists in Laboratory Medicine under EU Directive 2013/55/EC (The Recognition of Professional Qualifications)

Author

Wieringa, Gijsbert, primary, Queraltó, Josep, additional, Homšak, Evgenija, additional, Jassam, Nuthar, additional, Cavalier, Etienne, additional, Svinarov, Dobrin, additional, Krleža, Jasna L, additional, Christou, Spyroula, additional, Pikner, Richard, additional, Larsen, Trine Rennebod, additional, Tomberg, Karel, additional, Linko-Parvinen, Anna, additional, Sapin, Vincent, additional, Baum, Hannsjorg, additional, Kroupis, Christos, additional, Bagoly, Zsuzsa, additional, Costelloe, Sean, additional, Sciacovelli, Laura, additional, Stasulans, Janis, additional, Vitkus, Dalius, additional, Meunier, Danielle, additional, Solnica, Bogdan, additional, Reguengo, Henrique, additional, Mambet, Cristina, additional, Kovac, Gustav, additional, Krhin, Blaž, additional, Ohlson, Mats, additional, Buhagiar, Gerald, additional, and Simundic, Ana-Maria, additional

Published
2020
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32. A proposed Common Training Framework for Specialists in Laboratory Medicine under EU Directive 2013/55/EC (The Recognition of Professional Qualifications).

Author

Wieringa, Gijsbert, Queraltó, Josep, Homšak, Evgenija, Jassam, Nuthar, Cavalier, Etienne, Svinarov, Dobrin, Krleža, Jasna L, Christou, Spyroula, Pikner, Richard, Larsen, Trine Rennebod, Tomberg, Karel, Linko-Parvinen, Anna, Sapin, Vincent, Baum, Hannsjorg, Kroupis, Christos, Bagoly, Zsuzsa, Costelloe, Sean, Sciacovelli, Laura, Stasulans, Janis, and Vitkus, Dalius

Subjects
CLINICAL pathology, MEDICAL specialties & specialists, EUROPEAN integration, PROFESSIONAL associations
Abstract

European Union (EU) Directive 2013/55/EC (The Recognition of Professional Qualifications) allows Member States to decide on a common set of minimum knowledge, skills and competences that are needed to pursue a given profession through a Common Training Framework. To be adopted the framework must combine the knowledge, skills and competences of at least one third of the Member States. Professionals who have gained their qualifications under a Common Training Framework will be able to have these recognised automatically within the Union. The backbone of the European Federation of Clinical Chemistry and Laboratory Medicine's (EFLM) proposed Common Training Framework for non-medical Specialists in Laboratory Medicine is outlined here. It is based on an Equivalence of Standards in education, training, qualifications, knowledge, skills, competences and the professional conduct associated with specialist practice. In proposing the recognition of specialist practice EFLM has identified 15 EU Member States able to meet Equivalence and in whom the profession and/or its training is regulated (an additional EU Commission requirement). The framework supports and contributes to the Directive's enabling goals for increasing professional mobility, safeguarding consumers and ensuring a more equitable distribution of skills and expertise across the Member States. It represents EFLM's position statement and provides a template for professional societies and/or competent authorities to engage with the EU Commission. [ABSTRACT FROM AUTHOR]

Published
2021
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39. TCT-319 Side Branch Mortality Score for Prediction of Five Year Mortality After Provisional Side Branch Coronary Bifurcation Stenting – a New Tool Based on Intracoronary Electrocardiogram (iECG) From iECG and Myonecrosis After Bifurcation Stenting (COSIBRIA&CO) (ClinicalTrials.gov Identifier:NCT01268228)

Author

Vassilev, Dobrin, primary, Karamfiloff, Kiril, additional, Dosev, Liubomir, additional, Stoykova, Jivka, additional, Naunov, Vladimir, additional, Pancheva, Ralitsa, additional, Zlatancheva, Galina, additional, Shumkova, Monika, additional, Svinarov, Dobrin, additional, Cakova, Adelina, additional, Rigatelli, Gianluca, additional, and Gil, Robert, additional

Published
2016
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40. TCT-45 Five Year Mortality Predictors After provisional T-stenting in Coronary Bifurcation Lesions, with Intracoronary Electrocardiogram Change Defined Strategy. From Intracoronary Electrocardiogram (ECG) and Myonecrosis After Bifurcation Stenting (COSIBRIA&CO) (ClinicalTrials.gov Identifier:NCT01268228)

Author

Vassilev, Dobrin, primary, Karamfiloff, Kiril, additional, Dosev, Liubomir, additional, Stoykova, Jivka, additional, Pancheva, Ralitsa, additional, Zlatancheva, Galina, additional, Shumkova, Monika, additional, Svinarov, Dobrin, additional, Cakova, Adelina, additional, Rigatelli, Gianluca, additional, and Gil, Robert, additional

Published
2016
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