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35 results on '"Synytsya, Alla"'

33. Novel Porphyrin Conjugates with a Potent Photodynamic Antitumor Effect: Differential Efficacy of Mono‐ and Bis‐β‐cyclodextrin Derivatives In Vifroand In Vivo

Author

Kralova, Jarmila, Synytsya, Alla, Pouckova, Pavla, Koc, Michal, Dvorak, Michal, and Kral, Vladimir

Abstract

In the present study we investigated the photosensitizing properties of two novel mono‐ and bis‐cyclodextrin tetrakis (pentafluorophenyl) porphyrin derivatives in several tumor cell lines and in BALB/c mice bearing subcutaneously transplanted syngeneic mouse mammary carcinoma 4T1. Both studied sensitizers were localized mainly in lysosomes and were found to induce cell death by triggering apoptosis in human leukemic cells HL‐60. In 4T1 and other cell lines both apoptotic and necrotic modes of cell death occurred depending on drug and light doses. Mono‐cyclodextrin porphyrin derivative P(β‐CD)l exhibited stronger in vitrophototoxic effect than bis‐cyclodextrin derivative P(β‐CD)2. However, in vivoP(β‐CD)2 displayed faster tumor uptake with maximal accumulation 6 h after application, leading to complete and prolonged elimination of subcutaneous tumors within 3 days after irradiation (100 J cm‐2). In contrast, P(β‐CD)1 uptake was slower (48 h) and the reduction of tumor mass was only transient, reaching the maximum at the 12 h interval when a favorable tumor‐to‐skin ratio appeared. Thus, P(β‐CD)2 represents a new photosensitizing drug displaying fast and selective tumor uptake, strong antitumor activity and fast elimination from the body.

Published
2006
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34. Modification of chitosan-methylcellulose composite films with meso-tetrakis(4-sulfonatophenyl)porphyrin.

Author

Synytsya A, Grafová M, Slepicka P, Gedeon O, and Synytsya A

Subjects
Absorption, Circular Dichroism, Drug Delivery Systems methods, Hydrogen-Ion Concentration, Microscopy, Electron, Scanning, Molecular Structure, Spectroscopy, Fourier Transform Infrared, Spectrum Analysis, Raman, Water, Biocompatible Materials chemical synthesis, Chitosan chemistry, Methylcellulose chemistry, Porphyrins chemistry
Abstract

Polysaccharide films containing chitosan, methylcellulose, and a mixture of these polysaccharides in various ratios were prepared and modified with meso-tetrakis(4-sulfonatophenyl)porphyrin in an aqueous medium at pH 7. The modified films were compared with the initial films using spectroscopic methods and microscopic imaging. Electronic (UV-vis absorption, electronic circular dichroism (ECD)) and vibrational (FTIR and Raman) spectra showed that the porphyrin macrocycles had a strong affinity toward chitosan and did not interact with the methylcellulose. The total porphyrin uptake depended on the chitosan: methylcellulose ratio and pure methylcellulose films did not retain porphyrin macrocycles. ECD measurements detected the presence of optically active porphyrin species bound to the films. SEM and AFM images confirmed that the porphyrin macrocycles caused structural changes on the film surface and within the film layer.

Published
2012
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35. Novel porphyrin conjugates with a potent photodynamic antitumor effect: differential efficacy of mono- and bis-beta-cyclodextrin derivatives in vitro and in vivo.

Author

Kralova J, Synytsya A, Pouckova P, Koc M, Dvorak M, and Kral V

Subjects
Animals, Cell Death drug effects, Cell Death radiation effects, Dermatitis, Phototoxic drug therapy, Humans, Lysosomes pathology, Mice, Mice, Inbred BALB C, Skin radiation effects, Time Factors, Tumor Cells, Cultured, Antineoplastic Agents pharmacology, Photosensitizing Agents pharmacology, Porphyrins pharmacology, Skin drug effects, beta-Cyclodextrins pharmacology
Abstract

In the present study we investigated the photosensitizing properties of two novel mono- and bis-cyclodextrin tetrakis (pentafluorophenyl) porphyrin derivatives in several tumor cell lines and in BALB/c mice bearing subcutaneously transplanted syngeneic mouse mammary carcinoma 4T1. Both studied sensitizers were localized mainly in lysosomes and were found to induce cell death by triggering apoptosis in human leukemic cells HL-60. In 4T1 and other cell lines both apoptotic and necrotic modes of cell death occurred depending on drug and light doses. Mono-cyclodextrin porphyrin derivative P(beta-CD)1 exhibited stronger in vitro phototoxic effect than bis-cyclodextrin derivative P(beta-CD)2. However, in vivo P(beta-CD)2 displayed faster tumor uptake with maximal accumulation 6 h after application, leading to complete and prolonged elimination of subcutaneous tumors within 3 days after irradiation (100 J cm(-2)). In contrast, P(beta-CD)1 uptake was slower (48 h) and the reduction of tumor mass was only transient, reaching the maximum at the 12 h interval when a favorable tumor-to-skin ratio appeared. Thus, P(beta-CD)2 represents a new photosensitizing drug displaying fast and selective tumor uptake, strong antitumor activity and fast elimination from the body.

Published
2006
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