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13. Bioproduction of l- and d-lactic acids: advances and trends in microbial strain application and engineering

Author

Augustiniene, Ernesta, Valanciene, Egle, Matulis, Paulius, Syrpas, Michail, Jonuskiene, Ilona, and Malys, Naglis

Subjects
food and beverages
Abstract

Lactic acid is an important platform chemical used in the food, agriculture, cosmetic, pharmaceutical, and chemical industries. It serves as a building block for the production of polylactic acid (PLA), a biodegradable polymer, which can replace traditional petroleum-based plastics and help to reduce environmental pollution. Cost-effective production of optically pure l- and d-lactic acids is necessary to achieve a quality and thermostable PLA product. This paper evaluates research advances in the bioproduction of l- and d-lactic acids using microbial fermentation. Special emphasis is given to the development of metabolically engineered microbial strains and processes tailored to alternative and flexible feedstock concepts such as: lignocellulose, glycerol, C1-gases, and agricultural-food industry byproducts. Alternative fermentation concepts that can improve lactic acid production are discussed. The potential use of inducible gene expression systems for the development of biosensors to facilitate the screening and engineering of lactic acid-producing microorganisms is discussed.

Published
2021
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18. Bioproduction of l- and d-lactic acids: advances and trends in microbial strain application and engineering.

Author

Augustiniene, Ernesta, Valanciene, Egle, Matulis, Paulius, Syrpas, Michail, Jonuskiene, Ilona, and Malys, Naglis

Subjects
LACTIC acid, BIODEGRADABLE plastics, POLYLACTIC acid, CHEMICAL industry, ENGINEERS, ACIDS, ENGINEERING, POLLUTION
Abstract

Lactic acid is an important platform chemical used in the food, agriculture, cosmetic, pharmaceutical, and chemical industries. It serves as a building block for the production of polylactic acid (PLA), a biodegradable polymer, which can replace traditional petroleum-based plastics and help to reduce environmental pollution. Cost-effective production of optically pure l- and d-lactic acids is necessary to achieve a quality and thermostable PLA product. This paper evaluates research advances in the bioproduction of l- and d-lactic acids using microbial fermentation. Special emphasis is given to the development of metabolically engineered microbial strains and processes tailored to alternative and flexible feedstock concepts such as: lignocellulose, glycerol, C1-gases, and agricultural-food industry byproducts. Alternative fermentation concepts that can improve lactic acid production are discussed. The potential use of inducible gene expression systems for the development of biosensors to facilitate the screening and engineering of lactic acid-producing microorganisms is discussed. [ABSTRACT FROM AUTHOR]

Published
2022
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23. N-acylated homoserine lactone-derived tetramic acids as algicidal compounds

Author

Backx, Simon, Stock genannt Schroer, Frederike, Graff van Creveld, Shiri, Syrpas, Michail, Blommaert, Lander, Stock, Willem, Ruysbergh, Ewout, Sabbe, Koen, De Kimpe, Norbert, Willems, Anne, Vardi, Assaf, Vyverman, Wim, and Mangelinckx, Sven

Subjects
Chemistry, Biology and Life Sciences
Published
2019

25. Oleogel formulation using lipophilic sea buckthorn extract isolated from pomace with supercritical CO2.

Author

Eisinaitė, Viktorija, Vinauskienė, Rimantė, Syrpas, Michail, Venskutonis, Petras Rimantas, and Leskauskaitė, Daiva

Subjects
SEA buckthorn, OMEGA-6 fatty acids, VEGETABLE oils, SUPERCRITICAL carbon dioxide, FAT substitutes, OMEGA-3 fatty acids, UNSATURATED fatty acids, POLYMER networks
Abstract

Lipophilic sea buckthorn pomace extract isolated by supercritical CO2 (LSBPE) was structurized with different amounts of carnauba wax or beeswax as oleogelators. Oleogels were also made with added water at an LSBPE:water ratio of 70:30. LSBPE was characterized by a favorable ratio of omega‐6 to omega‐3 fatty acids (1:3) and reasonable amounts of omega‐7 fatty acids (4.45% of total), tocopherols (63.0 mg/100 g) and carotenoids (700.1 mg/100 g). The oleogels were characterized by their structural and rheological properties, and physical and chemical stability during storage. Carnauba wax produced a gel‐like structure with a highly condensed network of aggregated crystals, while beeswax oleogels displayed elongated crystals which formed interconnected networks. Carnauba wax gels were harder than beeswax gels with better oil‐binding capacity and higher crystallization and melting temperatures. Both oleogels showed good physical and oxidative stability during storage due to the immobilization of oil in the three‐dimensional structures, and the presence of strong lipophilic antioxidants in LSBPE, respectively. As the proportion of waxes increased from 5 to 17.5%, the hardness, oil‐binding capacity, crystallization, and melting temperatures increased in both gels due to the more developed crystalline associations. Addition of water had no significant effect on the physical and chemical stability of oleogels during storage. Water was distributed as small droplets in the crystalline network of oleogelators. However, hardness and oil‐binding capacity decreased as water was added. Rich in polyunsaturated fatty acids and bioactive compounds, LSBPE oleogels offer the opportunity to deliver bioactives while simultaneously acting as fat substitutes. [ABSTRACT FROM AUTHOR]

Published
2021
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27. N-Acyl Homoserine Lactone Derived Tetramic Acids Impair Photosynthesis in Phaeodactylum tricornutum

Author

Stock, Frederike, primary, Syrpas, Michail, additional, Graff van Creveld, Shiri, additional, Backx, Simon, additional, Blommaert, Lander, additional, Dow, Lachlan, additional, Stock, Willem, additional, Ruysbergh, Ewout, additional, Lepetit, Bernard, additional, Bailleul, Benjamin, additional, Sabbe, Koen, additional, De Kimpe, Norbert, additional, Willems, Anne, additional, Kroth, Peter G., additional, Vardi, Assaf, additional, Vyverman, Wim, additional, and Mangelinckx, Sven, additional

Published
2019
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28. BIOCONVERSION OF WASTE BREAD TO GLUCOSE FRUCTOSE SYRUP AS A VALUE-ADDED PRODUCT.

Author

Riaukaite, Julija, Basinskiene, Loreta, and Syrpas, Michail

Subjects
SYRUPS, FOOD industrial waste, FRUCTOSE, COOKING
Abstract

Bread is one of the most wasted products of all food in many countries around the world. Bread waste is a resource of carbohydrates, proteins and lipids which can be reused in order to get value-added products. In this study, bread residues were converted into a glucose syrup via two stage enzymatic hydrolysis. The optimization process and response surface methodology were used to find the optimal substrate, water and enzyme ratio to produce the highest yields of fermentable sugars. The effects of bread (11.34-28.66 g 100 g-1), a-amylase (0.013-0.047 KNU g-1 bread) and glucoamylase (0.23-0.57 AGU g-1 bread) loadings were investigated at liquefaction and saccharification stages. Results indicated that the amount of both enzymes was significant and determines the final glucose yield. Isomerization was performed for glucose conversion to fructose using optimal amounts of materials determined in hydrolysis experiments. Another optimization process was performed to determine the most effective amount of glucose isomerase enzyme (2.59-5.41 IGIU g-1 syrup) and pH (6.79-8.21). The increase in enzyme loading and pH up to 8 accelerated isomerization reaction. With the optimal process parameters, the highest fructose yield was achieved (40.32%). Hydrolysis and isomerization processes show bread waste potential of being a resource for the bioproduction of higher value products. [ABSTRACT FROM AUTHOR]

Published
2019
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29. Ultrasound-Assisted Extraction and Assessment of Biological Activity of Phycobiliprotein-Rich Aqueous Extracts from Wild Cyanobacteria (Aphanizomenon flos-aquae)

Author

Syrpas, Michail, Bukauskaitė, Jolita, Ramanauskienė, Kristina, Karosienė, Ju̅ratė, Majienė, Daiva, Bašinskienė, Loreta, and Venskutonis, Petras Rimantas

Abstract

Cyanobacteria are photosynthetic microorganisms that are considered as an important source of bioactive metabolites, among which phycobiliproteins (PBPs) are a class of water-soluble macromolecules of cyanobacteria with a wide range of applications. Massive proliferation of cyanobacteria can lead to excessive surface water blooms, of which removal, as a management measure, should be prioritized. In this study, the utilization of wild cyanobacteria biomass (Aphanizomenon flos-aquae) for extraction of phycobiliproteins is reported. Extraction of phycobiliproteins by conventional methods, such as homogenization, freeze–thaw cycles, and solid–liquid extraction, were optimized prior to ultrasound-assisted extraction. Standardization of ultrasonication for different parameters, such as ultrasonication amplitude (38, 114, and 190 μm) and ultrasonication time (1, 5.5, and 10 min), was carried out using a central composite design and response surface methodology for each of the primary techniques. A substantial increase on the individual and total phycobiliprotein yields was observed after ultrasonic treatment. The highest total PBP yield (115.37 mg/g of dry weight) was observed with samples treated with a homogenizer (30 min, 30 °C, and 1 cycle) combined with ultrasound treatment (8.7 min at 179 μm). Moreover, in vitroantioxidant capacity was observed for the obtained extracts in the Folin–Ciocalteu and ABTS*+assays. In addition, a cytotoxic effect against C6 glioma cells was observed for A. flos-aquaePBPs. Conclusively, wild cyanobacteria could be considered as an alternative feedstock for recovery of PBPs.

Published
2020
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30. Modeling and optimization of supercritical carbon dioxide extraction for isolation of valuable lipophilic constituents from elderberry (Sambucus nigraL.) pomace

Author

Kitrytė, Vaida, Laurinavičienė, Agnė, Syrpas, Michail, Pukalskas, Audrius, and Venskutonis, Petras Rimantas

Abstract

•High pressure, ultrasound and traditional extractions tested for elderberry pomace.•Supercritical CO2extraction parameters were optimised using SSC based RSM.•The yield of lipophilic substances at 53 °C /35 MPa / 45 min was 14.05%.•Extracted oil consisted mainly of linoleic (42%) and α-linolenic (34%) fatty acids.•Toxic sambunigrin content in extracts was very low, 0.5-0.7 ng/g.

Published
2020
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31. Short-chain fructooligosaccharides synthesis using a commercial enzyme complex from Aspergillussp. and anti-cancer activity on HCT116 and HT-29 cell lines

Author

Bytautaitė, Meda, Kitrytė-Syrpa, Vaida, Jonutė, Paulina, Endriulaitytė, Ugne, Petrikaitė, Vilma, and Syrpas, Michail

Abstract

Over the last years, short-chain fructooligosaccharides (FOS) have gained significant attention as valuable food and dietary supplement components. Apart from implications such as dietary fibres, sweeteners, and humectants, FOS are hailed for their prebiotic properties. Moreover, much research is dedicated to their role as anti-cancer agents. Despite the ongoing interest, both technical aspects, challenges of FOS production, and their anti-cancer role necessitate more in-depth investigations to understand their healthcare role better. This study aimed to optimise critical FOS synthesis parameters using a commercial enzyme, Viscozyme® L, to obtain a preparation with a high FOS content. A central composite design and response surface methodology evaluated the effect of pH, temperature, synthesis time, substrate, and enzyme load on multiple responses, chosen as independent variables, were utilised to define optimal conditions. Under the optimal conditions (temperature 50 °C, pH 5.5, sucrose concentration 352 g/L, enzyme concentration 2.5% v/v, and 5.5 hours of reaction), a preparation with a total FOS content of 58.8% was obtained. The FOS preparation showed minimal in vitroantioxidant capacity in the ORAC, CUPRAC, and ABTS* +assays. In addition, the cytotoxicity of FOS against tested cell lines was evaluated. FOS did not reduce HT-29 cell viability even at the concentration of 2.5 mg/mL, whereas FOS inhibited the proliferation of HCT116 cells by 50% at 2.35 mg/mL. Finally, the sensitisation effect of FOS on HCT116 cell treatment with doxorubicin has been revealed.

Published
2024
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32. EVALUATION OF PHYSICOCHEMICAL, PHENOLIC PROFILE AND ANTIOXIDANT CAPACITY CHANGES OF KOMBUCHA DURING FERMENTATION.

Author

Minelgaite, Vidmante and Syrpas, Michail

Subjects
KOMBUCHA tea, OXIDANT status, FERMENTATION
Abstract

Kombucha is a slightly sweet, tangy, and effervescent fermented beverage that has recently become popular due to its reported health advantages. However, more research is needed to understand these potential benefits fully. This study aimed to determine the physicochemical and bioactive compound changes and the total antioxidant capacity of black tea kombucha during fermentation. The pH and total acidity were evaluated during sixteen fermentation days. As could be expected, pH reduced from an initial 4.70 ± 0.03 to 2.86 ± 0.03 while total acidity increased from 0.60 ± 0.04 to 11.62 ± 0.20 g L-1. High performance liquid chromatography in tandem mass spectroscopy (HPLC-MS/MS) was successfully developed to analyze the changes of target constituents (carbohydrates, organic acids, phenolic compounds), resulting in the detection of twenty-four compounds. HPLCMS/MS results further verified that lactic and acetic acid content increased as fermentation progressed. Sucrose content decreased, while changes in the most abundant phenolic substances revealed different patterns. The total phenolic content (TPC) and antioxidant capacity were evaluated with the Folin-Ciocalteu method, ABTS radical scavenging and CUPRAC assays. During fermentation, the TPC increased from 525 ± 3 (day 0) to 721 ± 2 µg GAE mL-1 (day 16th). In the ABTS assay, the capacity of kombucha increased from 1527 ± 146 to 1767 ± 35 mg TE mL-1 by the 9th day, then decreased to 1467 ± 123 mg TE mL-1 by the 16th day. In contrast, no significant changes in the antioxidant capacity were observed in the CUPRAC assay. Understanding and analyzing physicochemical and bioactive compound changes in kombucha during fermentation can help develop kombuchas with higher bioactive potential and improve knowledge on the kombucha fermentation process. [ABSTRACT FROM AUTHOR]

Published
2023

33. PRESSURIZED LIQUID EXTRACTION OF BIOACTIVE COMPOUNDS FROM PARMOTREMA PERLATUM: OPTIMIZATION AND ANALYSIS.

Author

Dit Al Mazraani, Rizkallah Al Akiki and Syrpas, Michail

Subjects
PARMELIACEAE, BIOACTIVE compounds, LIQUID chromatography
Abstract

Parmotrema perlatum (family Parmeliaceae), also known as Black stone or stone flower, is a lichen found in the Southern and Northern hemispheres and is utilized as a spice in India and China. Although it is documented that many lichen-derived substances possess a broad spectrum of bioactivity, studies on characterization and especially the recovery of bioactive components utilizing sustainable extraction technologies are relatively scarce. This study evaluated pressurized liquid extraction (PLE) as a sustainable technique for recovering lichen-specific functional components. Firstly, liquid chromatography with tandem mass spectrometry (LC-MS/MS) indicated that lichen extracts were characterized by lecanoric-, gyrophoric- and usnic acid. In that direction, a central composite design and response surface methodology was used to assess the effect of extraction parameters, including solvent (ethyl acetate-ethanol), time (15-45 min) and temperature (40-120 °C) on the recovery of the three main components, chosen as response factors. The highest concentrations for lecanoric- (ca. 350 mg g-1 of extract), gyrophoric- (ca. 287 mg g-1 of extract), and usnic acid (ca. 60 mg g-1 of extract) were observed with ethyl acetate. Analysis of variance indicated that, the extraction temperature was a critical factor, with high recoveries recorded under mild conditions (40-50 °C). Furthermore, lecanoric and gyrophoric acid extraction was favored by short extraction times, whereas usnic acid required more extended extraction periods. After numerical optimization under the selected constraints and considering all observed responses, the optimal extraction conditions were (pure ethyl acetate, 45 °C, and 24 min), under which all quadratic models were validated. Besides targeted substances, LC-MS/MS data revealed the occurrence of other depside and dibenzofuran derivatives. Conclusively, this study highlights the high efficiency of PLE in recovering rapidly fractions with high concentrations of the targeted bioactive molecules under mild conditions. [ABSTRACT FROM AUTHOR]

Published
2023

34. EVALUATION OF COLOUR STABILITY OF PURIFIED PHYCOCYANIN EXTRACTED FROM ARTHROSPIRA PLATENSIS AFTER COMPLEXATION WITH FOOD-GRADE POLYSACCHARIDES.

Author

Krakauskaite, Ugne and Syrpas, Michail

Subjects
PHYCOCYANIN, POLYSACCHARIDES, SPIRULINA
Abstract

Phycocyanin is a blue-coloured phycobiliprotein found in cyanobacteria with great potential to be used as a natural colourant in the food industry. The use of this dye is limited since phycocyanin is relatively unstable, affected by temperature, pH, and light exposure, as well as extraction and purification. This study aimed to obtain a high-purity extract of phycocyanin and evaluate the use of food-grade polysaccharide complexation to improve the stability of the blue colour under different storage conditions. Towards this, various extraction conditions (time, extraction solvent, biomass to solvent ratio) and conventional extraction techniques such as homogenisation, freeze-thaw and maceration were evaluated for their ability to recover phycocyanin from Arthrospira platensis biomass. The highest phycocyanin yield was obtained after homogenisation with phosphate buffer (0.1M, pH 7) for 45 minutes (37.01 mg g-1 DW, purity 0.82), while maceration with phosphate buffer (0.1M, pH 7) for 2 hours resulted in lower yield (30.73 mg g-1 DW) but showed the highest purity extract (1.35). Further extract purification was performed, and using activated carbon resulted in the highest level of purification, with the final purity reaching 2.58 (food-grade). Afterwards, the phycocyanin-rich extract was complexed with food-grade polysaccharides (xanthan gum, iotacarrageenan) to evaluate its stability under different storage conditions (temperature, packaging, light exposure, heat treatment). Phycocyanin stability was improved with additives, with xanthan gum showing the most significant effect on the stability of phycocyanin content throughout storage. Conclusively, adding polysaccharides to phycocyanin solutions can potentially improve colour stability over storage time. [ABSTRACT FROM AUTHOR]

Published
2023

37. Contributors

Author

Aguilar-Juárez, Oscar, Allais, Florent, Arellano-García, Luis, Ayala-Mendivil, Neydeli, Barrera-Martínez, Iliana, Cabrera-Barjas, Gustavo, Casas-Godoy, Leticia, Castaño, Patricia, Castillo, Catalina, Correa, C. Rodriguez, Demedts, Brecht, De Smet, David, Fatima Charrier - El Bouhtoury, Gimeno, P. J. Arauzo, Glass, David J., Goethals, Frederik, Hoffmann, V., Issaoui, Hamed, Kruse, A., Langeveld, J.W.A., Méndez-Zamora, Andrés, Moreno, Antonio D., Mouterde, Louis M.M., Musa, B., Negro, María José, Nesic, Aleksandra, Oliva, José Miguel, Olszewski, M.P., Reyes, Ana Laura, Sanders, J.P.M., Sandoval, Georgina, Serrano, Jesus, Susmozas, Ana, Swiatek, K.M., Syrpas, Michail, Uyttendaele, Willem, Vanneste, Myriam, and Venskutonis, Petras Rimantas

Published
2020
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38. Optimized Supercritical CO 2 Extraction Enhances the Recovery of Valuable Lipophilic Antioxidants and Other Constituents from Dual-Purpose Hop (Humulus lupulus L.) Variety Ella.

Author

Nagybákay, Nóra Emilia, Syrpas, Michail, Vilimaitė, Vaiva, Tamkutė, Laura, Pukalskas, Audrius, Venskutonis, Petras Rimantas, and Kitrytė, Vaida

Subjects
RESPONSE surfaces (Statistics), CARBON dioxide, HOPS, REACTIVE oxygen species, SUPERCRITICAL carbon dioxide, ANTIOXIDANTS
Abstract

The article presents the optimization of supercritical CO2 extraction (SFE-CO2) parameters using response surface methodology (RSM) with central composite design (CCD) in order to produce single variety hop (cv. Ella) extracts with high yield and strong in vitro antioxidant properties. Optimized SFE-CO2 (37 MPa, 43 °C, 80 min) yielded 26.3 g/100 g pellets of lipophilic fraction. This extract was rich in biologically active α- and β-bitter acids (522.8 and 345.0 mg/g extract, respectively), and exerted 1481 mg TE/g extract in vitro oxygen radical absorbance capacity (ORAC). Up to ~3-fold higher extraction yield, antioxidant recovery (389.8 mg TE/g pellets) and exhaustive bitter acid extraction (228.4 mg/g pellets) were achieved under the significantly shorter time compared to the commercially used one-stage SFE-CO2 at 10–15 MPa and 40 °C. Total carotenoid and chlorophyll content was negligible, amounting to <0.04% of the total extract mass. Fruity, herbal, spicy and woody odor of extracts could be attributed to the major identified volatiles, namely β-pinene, β-myrcene, β-humulene, α-humulene, α-selinene and methyl-4-decenoate. Rich in valuable bioactive constituents and flavor compounds, cv. Ella hop SFE-CO2 extracts could find multipurpose applications in food, pharmaceutical, nutraceutical and cosmetics industries. [ABSTRACT FROM AUTHOR]

Published
2021
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39. Oleogel formulation using lipophilic sea buckthorn extract isolated from pomace with supercritical CO2.

Author

Eisinaitė, Viktorija, Vinauskienė, Rimantė, Syrpas, Michail, Venskutonis, Petras Rimantas, and Leskauskaitė, Daiva

Subjects
*SEA buckthorn, *OMEGA-6 fatty acids, *VEGETABLE oils, *SUPERCRITICAL carbon dioxide, *FAT substitutes, *OMEGA-3 fatty acids, *UNSATURATED fatty acids, *POLYMER networks
Abstract

Lipophilic sea buckthorn pomace extract isolated by supercritical CO2 (LSBPE) was structurized with different amounts of carnauba wax or beeswax as oleogelators. Oleogels were also made with added water at an LSBPE:water ratio of 70:30. LSBPE was characterized by a favorable ratio of omega‐6 to omega‐3 fatty acids (1:3) and reasonable amounts of omega‐7 fatty acids (4.45% of total), tocopherols (63.0 mg/100 g) and carotenoids (700.1 mg/100 g). The oleogels were characterized by their structural and rheological properties, and physical and chemical stability during storage. Carnauba wax produced a gel‐like structure with a highly condensed network of aggregated crystals, while beeswax oleogels displayed elongated crystals which formed interconnected networks. Carnauba wax gels were harder than beeswax gels with better oil‐binding capacity and higher crystallization and melting temperatures. Both oleogels showed good physical and oxidative stability during storage due to the immobilization of oil in the three‐dimensional structures, and the presence of strong lipophilic antioxidants in LSBPE, respectively. As the proportion of waxes increased from 5 to 17.5%, the hardness, oil‐binding capacity, crystallization, and melting temperatures increased in both gels due to the more developed crystalline associations. Addition of water had no significant effect on the physical and chemical stability of oleogels during storage. Water was distributed as small droplets in the crystalline network of oleogelators. However, hardness and oil‐binding capacity decreased as water was added. Rich in polyunsaturated fatty acids and bioactive compounds, LSBPE oleogels offer the opportunity to deliver bioactives while simultaneously acting as fat substitutes. [ABSTRACT FROM AUTHOR]

Published
2021
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40. Processing of Phaeodactylum tricornutum biomass into valuable functional ingredients by high-pressure extraction techniques

Author

Khakimova, Nadiia and Syrpas, Michail

Subjects
Phaeodactylum tricornutum, supercritical carbon dioxide extraction, response surface methodology, slėgio skysčio ekstrahavimas, karotinoidai, microalgae, pressurized liquid extraction, carotenoids, superkritinis anglies dioksido ekstrahavimas, mikrodumbliai, Phaeodactylum tricornutum
Abstract

Phaeodactylum tricornutum, the only species in the genus Phaeodactylum, belongs to a significant group of microalgae called diatoms. P. tricornutum is considered a promising feedstock of high-value products. Mainly, due to its ability to synthesize polyunsaturated fatty acids, like eicosapentaenoic acid, and carotenoids, especially fucoxanthin – a valuable pigment with multiple biological activities. This work aimed to develop multi-step P. tricornutum processing schemes for isolation of high-added value fractions using conventional and high-pressure extraction techniques. Various standard methods achieved the determination of chemical composition. Conventional and high-pressure extraction techniques like solid-liquid extraction (SLE), supercritical fluid extraction (SFE), and pressurized liquid extraction (PLE) were applied using various solvents in order to obtain extracts of various polarity. The high-pressure extraction techniques, SFE and PLE, were optimized using response surface methodology (RSM) in order to maximize total yield, carotenoid, and fucoxanthin content. In vitro antioxidant capacity of initial material, as well as all extracts were determined using the total phenolic content (by the Folin-Ciocalteu method), DPPH• and ABTS•+ assays. The activity of solid residues was tested with the same in vitro antioxidant capacity assays following the QUENCHER approach. The qualitative determination of pigments and quantitative determination of fucoxanthin content was performed using HPLC-DAD. The fatty acid profile was determined using GC-FID. Moreover, for all extracts, the antimicrobial activity was using the disc diffusion assay, determining the minimal inhibitory concentration for the active ones. This work demonstrates the feasibility of high-pressure techniques for the isolation of high-added value products from this rather underutilized feedstock., Phaeodactylum tricornutum yra vienintelė Phaeodactylum genties rūšis, priklausanti mikrodumblių, vadinamų diatomomis, grupei. Dėl savo unikalios sudėties P. tricornutum yra laikoma puikiu šaltiniu, siekiant gauti aukštos kokybės produktus. Šie mikrodumbliai geba sintetinti polinesočiąsias riebalų rūgštis, tokias kaip eikozapentaeno rūgštis, ir karotenoidus, ypač fukoksantiną – pigmentą, kuris pasižymi įvairiapusiu biologiniu aktyvumu. Šio darbo tikslas buvo sukurti daugiapakopes P. tricornutum perdirbimo schemas, skirtas išgauti aukštos prodėtinės vertės frakcijas, naudojant tradicinius ir didelio slėgio ekstrakcijos metodus. Cheminė sudėtis nustatyta naudojant įvairius standartinius metodus. Norint gauti skirtingo poliškumo ekstraktus panaudoti tradiciniai ir didelio slėgio ekstrakcijos metodai, tokie kaip maceravimas (SLE), superkritinė ekstrakcija anglies dvidegieniu (SFE) ir ekstrakcija padidinto slėgio aplinkoje (PLE), naudojant įvairius tirpiklius. Didelio slėgio ekstrakcijos metodai (SFE ir PLE) optimizuoti taikant paviršiaus atsako metodologiją (RSM), siekiant gauti didžiausią išeigą, karotenoidų ir fukoksantino kiekį. Antioksidacinis aktyvumas (in vitro) pradinėje žaliavoje bei visuose ekstraktuose įvertintas nustatant bendrą fenolinių junginių kiekį Folin-Ciocalteu‘s metodu bei naudojant DPPH• radikalo ir ABTS•+ katijono radikalo blukinimo metodus. Antioksidacinis pajėgumas liekanose po atliktų ekstrakcijų nustatytas naudojant tuos pačius metodus, tačiau pritaikant QUENCHER procedūrą. Pigmentų kokybinis bei fukoksantino kiekybinis įvertinimas atliktas naudojant HPLC-DAD. Riebalų rūgščių sudėtis nustatyta naudojant GC-FID. Visų ekstraktų antimikrobinis aktyvumas įvertintas naudojant difuzijos į agarą metodą bei nustatant minimalią slopinančią koncentraciją. Šis darbas parodo didelio slėgio ekstrakcijos metodų, galinčių išskirti aukštos pridėtinės vertės produktus iš šios nepakankamai išnaudotos žaliavos, galimybes.

Published
2020

41. Isolation of higher value-added components from Potentilla fruticosa and Hierochloe odorata by means of high-pressure and enzyme-assisted extraction techniques

Author

Subbarayadu, Kiran and Syrpas, Michail

Subjects
PLE, EAE, P. fruticosa, H. odorata, SFE-CO2
Abstract

Potentilla fruticosa is a shrubby perennial plant, native to Northern areas of America and Europe. It is commonly consumed as an herbal tea and exhibits great antioxidant potential as well as antifungal, antibacterial and anti-inflammatory properties. Hierochloe odorata is an aromatic perennial grass, widely distributed in West Asia and Europe, whose roots and aerial parts have a sweet smell. H. odorata extracts have shown to have high antioxidant activity and insect-repellent properties. The aim of this work was to isolate and analyze functional characterization of high-added value fractions of P. fruticosa and H. odorata through the development of biorefining schemes utilizing conventional, high-pressure, and enzymatic treatment techniques. The chemical composition of P. fruticosa and H. odorata was studied using various basic methods. Conventional and high-pressure extraction techniques like Solid liquid extraction and supercritical carbon dioxide extraction, pressurized liquid extraction and enzyme assisted extraction were applied on P. fruticosa and H. odorata. Defatted P. fruticosa and H. odorata residues after supercritical carbon dioxide extraction were used for further extraction techniques. PLE conditions were optimized using response surface methodology to maximize the extraction yield and phenolic content. Enzyme assisted extractions of defatted plant material and water residue from PLE of P. fruticosa and H. odorata were performed. Total phenolic content was determined using Folin-Ciocalteu’s technique and antioxidant activity was measured by DPPH• and ABTS•+ assays. Initial solid plants materials, SLE and SFE-CO2 residue antioxidant activity were analyzed using QUENCHER procedure. SLE, SFE-CO2, PLE, enzyme treated extracts total phenolic content was determined using Folin-Ciocalteu’s technique. Antioxidant activity was measured by DPPH• and ABTS•+ assays. The phytochemical characterization of volatile compounds of P. fruticosa and H. odorata SFE-CO2 extracts were analyzed by GCxGC/TOF MS, chemical compounds of P. fruticosa and H. odorata PLE extracts were analyzed using UPLC/ESI-QTOF-MS/MS., Aukštesnės pridėtinės vertės komponentų išskyrimas iš sidabražolės (Potentilla fruticosa) ir kvapiosios stumbražolės (Hierochloe odorata) taikant aukšto slėgio ir fermentinės ekstrakcijos metodus. Potentilla fruticosa yra daugiametis krūminis augalas, kilęs iš Šiaurės Amerikos ir Europos, vartojamas kaip žolelių arbata bei pasižymintis dideliu antioksidaciniu potencialu, taip pat priešgrybelinėmis, antibakterinėmis ir priešuždegiminėmis savybėmis. Hierochloe odorata yra aromatinė daugiametė žolė, plačiai paplitusi Vakarų Azijoje ir Europoje, kurios šaknys ir plaukeliai turi saldų kvapą. H. odorata ekstraktai turi didelį antioksidacinį potencialą ir pasižymi vabzdžius atbaidančiomis savybėmis. Šio darbo tikslas yra išskirti ir išanalizuoti aukštesnės pridėtinės vertės P. fruticosa ir H. odorata frakcijų funkcinę charakteristiką, sukuriant jų biorafinavimo schemas taikant tradicinius, aukšto slėgio ir fermentinės ekstrakcijos metodus. Cheminė P. fruticosa ir H. odorata sudėtis buvo tiriama naudojant įvairius pagrindinius metodus. P. fruticosa ir H. odorata buvo tiriamios tradiciniais ir aukšto slėgio ekstrakcijos metodais, naudojant maceraciją (SLE), superkrizinę skysčių ekstrakciją anglies dvideginiu (SFE-CO2), ekstrakciją suspaustais skysčiais (PLE) bei fermentinę hidrolizę. Po superkrizinės skysčių ekstrakcijos nuriebalintos P. fruticosa ir H. odorata liekanos buvo naudojamos tolesnėms ekstrakcijoms. PLE sąlygos buvo optimizuotos naudojant paviršiaus atsako metodiką, siekiant maksimaliai padidinti ekstrakcijos išeigą ir fenolinių junginių kiekį. Atlikta P. fruticosa ir H. odorata pradinės žaliavos ir nuriebalintų vandeninių liekanų po PLE fermentinė hidrolizė. Bendras fenolinių junginių kiekis buvo nustatytas naudojant Folin-Ciocalteu reagentą, o antioksidaciniam potencialui įvertinti naudoti DPPH• ir ABTS•+ metodai. Pradinės žaliavos, SLE ir SFE-CO2 liekanų antioksidacinis potencialas buvo nustatytas naudojant QUENCHER procedūrą. SLE, SFE-CO2, PLE ir fermentais apdorotų ekstraktų bendras fenolinių junginių kiekis taip pat buvo nustatytas naudojant Folin-Ciocalteu reagentą. Antioksidacinis aktyvumas buvo nustatytas DPPH• ir ABTS•+ metodais. P. fruticosa ir H. odorata SFE-CO2 ekstraktų lakiųjų komponentų fitocheminė sudėtis buvo analizuojama GCxGC/TOF MS, o PLE ekstraktų cheminė sudėtis buvo analizuojama naudojant UPLC/ESI-QTOF-MS/MS .

Published
2019

42. Health benefits and risks of fermented foods-the PIMENTO initiative.

Author

Todorovic S, Akpinar A, Assunção R, Bär C, Bavaro SL, Berkel Kasikci M, Domínguez-Soberanes J, Capozzi V, Cotter PD, Doo EH, Gündüz Ergün B, Guzel M, Harsa HS, Hastaoglu E, Humblot C, Hyseni B, Hosoglu MI, Issa A, Karakaş-Budak B, Karakaya S, Kesenkas H, Keyvan E, Künili IE, Kütt ML, Laranjo M, Louis S, Mantzouridou FT, Matalas A, Mayo B, Mojsova S, Mukherjee A, Nikolaou A, Ortakci F, Paveljšek D, Perrone G, Pertziger E, Santa D, Sar T, Savary-Auzeloux I, Schwab C, Starowicz M, Stojanović M, Syrpas M, Tamang JP, Yerlikaya O, Yilmaz B, Malagon-Rojas J, Salminen S, Frias J, Chassard C, and Vergères G

Abstract

Worldwide, fermented foods (FF) are recognized as healthy and safe. Despite the rapid increase of research papers, there is a lack of systematic evaluation of the health benefits and risks of FF. The COST Action CA20128 "Promoting innovation of fermented foods" (PIMENTO) aims to provide a comprehensive assessment on the available evidence by compiling a set of 16 reviews. Seven reviews will cover clinical and biological endpoints associated with major health indicators across several organ systems, including the cardiovascular, gastrointestinal, neurological, immune, and skeletal systems. Nine reviews will address broader biological questions associated with FF including bioactive compounds and vitamin production, nutrient bioavailability and bioaccessibility, the role of FF in healthy diets and personalized nutrition, food safety, regulatory practices, and finally, the health properties of novel and ethnic FF. For each outcome assessed in the reviews, an innovative approach will be adopted based on EFSA's published guidance for health claim submissions. In particular, each review will be composed of three parts: (1) a systematic review of available human studies; (2) a non-systematic review of the mechanism of action related to the clinical endpoints measured by the human studies identified in part 1; and (3) a non-systematic review of the characterization of the FF investigated in the human studies identified in part 1. The evidence and research gaps derived from the reviews will be summarized and published in the form of a strategic road map that will pave the way for future research on FF., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision., (Copyright © 2024 Todorovic, Akpinar, Assunção, Bär, Bavaro, Berkel Kasikci, Domínguez-Soberanes, Capozzi, Cotter, Doo, Gündüz Ergün, Guzel, Harsa, Hastaoglu, Humblot, Hyseni, Hosoglu, Issa, Karakaş-Budak, Karakaya, Kesenkas, Keyvan, Künili, Kütt, Laranjo, Louis, Mantzouridou, Matalas, Mayo, Mojsova, Mukherjee, Nikolaou, Ortakci, Paveljšek, Perrone, Pertziger, Santa, Sar, Savary-Auzeloux, Schwab, Starowicz, Stojanović, Syrpas, Tamang, Yerlikaya, Yilmaz, Malagon-Rojas, Salminen, Frias, Chassard and Vergères.)

Published
2024
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43. SYNTHESIS AND BIOLOGICAL EVALUATION OF NATURALLY AND NON-NATURALLY OCCURRING HALOGENATED ANALOGUES OF N-ACYL HOMOSERINE LACTONES AS QUORUM SENSING MODULATORS.

Author

Syrpas M, Ruysbergh E, Blommaert L, Vanelslander B, Sabbe K, Vyverman W, Stevens C, De Kimpe N, and Mangelinckx S

Subjects
Acyl-Butyrolactones chemistry, Chromobacterium metabolism, Escherichia coli metabolism, Green Fluorescent Proteins, Acyl-Butyrolactones metabolism, Diatoms metabolism, Quorum Sensing physiology
Published
2015

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