23 results on '"TÜRKSOY, Özlem"'
Search Results
2. Low-power holmium:YAG laser urethrotomy for urethral stricture disease: Comparison of outcomes with the cold-knife technique
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Atak, Mustafa, Tokgöz, Hüsnü, Akduman, Bülent, Erol, Bülent, Dönmez, İbrahim, Hancı, Volkan, Türksoy, Özlem, and Mungan, Necmettin Aydın
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- 2011
- Full Text
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3. Pain Perception During Shock Wave Lithotripsy: Does It Correlate With Patient and Stone Characteristics?
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Tokgöz, Hüsnü, Hanci, Volkan, Türksoy, Özlem, Erol, Bülent, Akduman, Bülent, and Mungan, Necmettin Aydin
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- 2010
- Full Text
- View/download PDF
4. Removal of heavy metals from textile industry wastewater
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TÜRKSOY, Recep, primary, TERZİOĞLU, Gökhan, additional, YALÇIN, İbrahim Ertuğrul, additional, TÜRKSOY, Özlem, additional, and DEMİR, Göksel, additional
- Published
- 2021
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- View/download PDF
5. Unusual clinical presentations of brucellosis
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Türksoy, ÖZLEM, Tokgöz, HÜSNÜ, and Toparli, SEMA
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- 2005
6. Kordomaya Moleküler Yaklaşımlar
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Tüysüz, Emre Can, Güllüoğlu, Şükrü, Türksoy, Özlem, Kuşkucu, Ayşegül, Bayrak, Ömer Faruk, Türe, Uğur, Tüysüz, Emre Can, Güllüoğlu, Şükrü, Türksoy, Özlem, Kuşkucu, Ayşegül, Bayrak, Ömer Faruk, Türe, Uğur, and Yeditepe Üniversitesi
- Subjects
Nörolojik Bilimler ,Cerrahi - Abstract
Kordoma, embriyonal notokord kalıntılarından köken alan, aksiyal iskelet boyunca yerleşim gösteren, genellikle düşük dereceli, kalsifiye olmuş ve lobüllerden meydana gelen kemik doku içinde yerleşimli bir iskelet tümörüdür. Bu derlemenin amacı kordoma tümör biyolojisi, tanı ve tedavisi konusunda modern genetik-moleküler biyolojik çalışmaların bulgularını özetlemektir. Kordoma moleküer biyolojisi konusundaki gelişmeler, hücre biyolojisini ilgilendiren farklı başlıklar altında ele alınmıştır. Kordomaların başlangıcında ve ilerlemesinde farklı çalışma gruplarının saptadığı genetik değişimler ve moleküler yolaklar üzerine ortaya çıkan görüşler açıklanmıştır. Kordomanın başlangıcı ve gelişimi konusundaki bilgimiz moleküler biyolojik yöntemlerin kullanımı sonrasında belirgin şekilde artmıştır. Fakat bu bilgi hastalığın daha etkin tedavisini sağlayabilecek noktaya gelmemiştir. Hastalığın daha iyi anlaşılabilmesi ve daha etkin tedavi yöntemlerinin uygulanabilmesi için kordomalardaki moleküler yolakların daha kapsamlı bir şekilde araştırılması gerekmektedir. A chordoma originates from remnants of the embryonic notochord and is located along the axial skeleton. This is a tumor of the skeleton that is usually low grade and is made of calcified lobules within the bone. This paper aims to review current molecular biological advances on the tumor-biology, diagnosis and treatment of chordomas. Advances in chordoma molecular biology are reviewed under different headings corresponding to various processes of cellular biology. Genetic and molecular biological findings of various study groups and the current take on these findings are summarized. Our understanding on the origin and development of chordomas has increases substantially after the application of molecular biological technology. However, these advancements have not yet resulted in novel and effective treatment strategies. Further studies are required to design effective treatment strategies for chordomas.
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- 2017
7. Protective Value of a Folkloric Medicinal Plant Extract Against Mortality and Hemorrhage in a Life-threatening Renal Trauma Model
- Author
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Tokgöz, Hüsnü, Karakaya, Kemal, Hancı, Volkan, Abduşoğlu, Mustafa, Erol, Bülent, Türksoy, Özlem, Akduman, Bülent, and Mungan, Necmettin Aydın
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- 2010
- Full Text
- View/download PDF
8. Epithelial to endothelial transition in glioblastoma: Angiogenesis
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Türksoy, Özlem, Bayrak, Ömer Faruk, and Biyoteknoloji Anabilim Dalı
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Brain neoplasms ,Biyoteknoloji ,DNA-mitochondrial ,Biotechnology - Abstract
Glioblastoma multiform (GBM) yetişkinlerde anormal vaskularizasyon ile birlikte seyreden, büyük oranda tedavi edilemeyen, en agresif ve invazif primer beyin tümörüdür. Tümör vaskularizasyonu tümör kitlesi içinde malign olmayan endotelyal hücrelerin oluşturduğu önceden var olan kan damarlarından yeni damarların oluşması olarak düşünülür. Vasküler mimikride (VM) ise vasküler kanallar tümörün içerisindeki agresif tümör hücreleri tarafından meydana getirilmektedir, bu olasılıkla anti anjiyojenik tedavinin başarısız olmasının sebebidir. GBM'de vaskulogenezin altında yatan moleküler mekanizmalar henüz aydınlatılmamış ise de glioblastomada hem VM hem de kanser kök hücrelerinin endotelyal hücrelere transdiferansiasyonu bildirilmiştir. Epitelyal-mezenkimal geçişi (EMT) tümör hücrelerinin metastatik potansiyelini arttmasında rol oynayan, dinamik ve tersine çevrilebilir bir süreçtir. Farklı çalışmalar EMT'nin anjiyogenez ve VM'de bir role sahip olabileceğini göstermiştir. Mitokondriyel DNA (mtDNA) deplesyonu kanserde habisliği belirleyen önemli bir etmendir. Gliomalarda mtDNA kaybı görülmekle birlikte, mtDNA kaybının EMT ile ilişkisi ve glioblastoma vaskulogenezindeki rolü bilinmemektedir. Bu çalışmada, glioblastoma hücrelerinde mtDNA kaybının EMT'deki ve izleyen vaskulogenik özelliklerde ve endotelyale farklılaşma potansiyelindeki artıştaki rolü araştırılmıştır. U87-MG ve A172 hücre hatları GBM modeli olarak kullanılmıştır. mtDNA deplesyonu için etidyum bromür (EtBr) uygulanmıştır. mtDNA kaybının mezenkimal hücre belirteçlerinin ekspresyonunu arttırarak EMT'yi indüklediği gösterilmiştir. Artan mezenkimal özelliklere uyumlu şekilde, Matrigel'de tübüler ağ oluşumu, transwell sisteminde invazyon, sferoid oluşumu ve endotelyal farklılaşma kapasitesi mtDNA'sı azaltılmış hücrelerde parental hücrelere göre artmıştır. Düşük mtDNA düzeylerinin EMT'yi indüklediği, VM özelliklerini ve endotelyal farklılaşma kapasitesini invazif özellik ile birlikte arttırdığı önerilmiştir. Bu çalışmada, mtDNA deplesyonu, GBM'de EMT'yi ve vaskulojenik özelliklerdeki artışı belirleyen önemli bir etken olarak bulunmuştur. mtDNA deplesyonu glioblastoma tedavisinde terapötik bir hedef olabilir. Glioblastoma multiforme (GBM) is highly incurable and one of the most aggressive and invasive primary brain tumors in adults, with abnormal vascularization. Tumor vascularization was considered as sprouting of new blood vessels from pre-existing ones composed of non-malignant endothelial cells into tumor mass. However, vasculogenic mimicry (VM) is defined as the generation of vascular channels via aggressive tumor cells in the tumor, probable responsible in the failure of anti-angiogenic therapies. Both VM and transdifferentiation of cancer stem cells into endothelial cells were reported in GBM. However, molecular mechanisms underlying the vasculogenesis of glioblastoma have not been fully identified yet. Epithelial-mesenchymal transition (EMT) is a dynamic, reversible process, playing causal role in the enhancement of metastatic potential of tumor cells. EMT may play role in angiogenesis and vasculogenic mimicry. Mitochondrial DNA (mtDNA) depletion is an important contributor to cancer malignancy. Although mtDNA depletion occurs in gliomas, the association between mtDNA depletion and EMT, their role in glioblastoma vasculogenesis is unknown. In this study, it was asked whether mtDNA depletion causes the acquisition of EMT phenotype and a subsequent increase in vasculogenic properties and differentiation potential into endothelial lineage in glioblastoma cells. U87-MG and A172 cell lines were used as GBM models. mtDNA was depleted using ethidium bromide (EtBr). mtDNA depletion induced EMT through upregulation of mesenchymal marker expression. Consistent with increased EMT properties, tubular network formation on Matrigel, invasion through a transwell system, spheroid formation and endothelial differentiation capacity were increased in mtDNA depleted cells compared to those in parental cells. Low mtDNA content induces EMT, increased VM properties and endothelial differentiation capacity together with invasiveness. In this study, mitochondrial stress was found as a crucial contributor for the acquisition of EMT and increase in vasculogenic properties in GBM, which may be a therapeutic target for glioblastoma treatment. 157
- Published
- 2017
9. Mitochondrial depletion in CD4 + and CD19 + peripheral lymphocytes in early stage Alzheimer’s disease
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Terzioğlu, Gökhan, primary, Örmeci, Burcu, additional, Türksoy, Özlem, additional, Sayman, Ceyhun, additional, Çınar, Nilgün, additional, Öztürk, Gökçen Akar, additional, and Demirel, Gülderen Yanıkkaya, additional
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- 2017
- Full Text
- View/download PDF
10. RENAL B-MOD ULTRASONOGRAFİ BULGULARININ DİABETİK NEFROPATİ EVRESİ İLE İLİŞKİSİ
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TÜRKSOY, Özlem, TOKGÖZ, Hüsnü, ELVERİCİ, Eda, DİLMAN, Şule, and YÜKSEL, Enis
- Subjects
Diabet,tip-2,nefropati,B-mod ultrasonografi - Abstract
Amaç: Çalışmamızda diabetik nefropati’nin farklı evrelerindeki hastalarda, B-mod Ultrasonografi (US) ile tespit edilen renal morfolojik özelliklerin kontrol grubu kullanılarak karşılaştırılması amaçlanmıştır. Yöntem: Kontrol grubu olarak 20 sağlıklı kişi alınmıştır. Çalışma grubu olarak ise tip-2 diabet tanısı olan 69 hasta alınmıştır. Heriki gruptaki hastalar böbreklerin boyutu; parenkim kalınlığı ve parenkim eko şiddeti yönünden B-mod US ile değerlendirilmiştir. Diabetik hastalar, 24 saatlik idrardaki albumin ekskresyon oranlarına (AEO) göre 3 gruba ayrılmıştır (Grup-1: AEO < 30 mg/ gün; Grup-2: AEO 30-300 mg/ gün; Grup- 3: AEO > 300 mg/ gün); ve her grup için ortalama renal parenkim kalınlığı ve renal uzunluk hesaplanmıştır. SPSS programı kullanılarak, bu değerler için gruplar arası karşılaştırma yapılmıştır. Bulgular: Böbrek boyutlarına göre, çalışma grubu ile kontrol grubu arasındaki farklılık araştırıldığında, istatistiksel olarak anlamlı farklılık gözlenirken, ortalama parenkim kalınlığının kontrol grubu ile hasta grupları arasında istatistiksel olarak farklı olmadığı görülmüştür. Hasta grupları, kendi içinde kıyaslandığında yine istatistiksel olarak anlamlı farklılık izlenmemiştir. Kortikal hiperekojenite saptanma oranlarının,ortalama serum BUN ve kreatinin klerens değerlerinin ise nefropati evresiyle korelasyonu izlenmiştir. Sonuç: Diabetik hastalarda, ilerleyen nefropati ile birlikte ultrasonografik olarak parenkim kalınlıkları anlamlı olarak değişmezken, eko şiddetinde artma ve sağlıklı bireylere göre böbrek boyutlarında azalma gerçekleşmektedir
- Published
- 2015
11. RENAL TRANSİZYONEL HÜCRELİ KARSİNOM İÇİN OLASI SERUM BELİRTEÇLERİ: LİTERATÜRÜN GÖZDEN GEÇİRİLMESİ İLE BİRLİKTE BİR OLGUNUN SUNUMU
- Author
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TOKGÖZ, Hüsnü, TÜRKSOY, Özlem, and EROL, Bülent
- Abstract
Üriner sistemin üst bölümünün ürotelyal tümörü, relatif olarak nadir görülen malignitelerden biridir. Prostat ve testis kanserlerine özgü serum belirteçleri bilinmesine rağmen, renal ve ürotelyal tümörler için spesifik belirteçlerin tanımlanmasına ihtiyaç vardır. Bu makalede, yüksek preoperatif serum düzeyine sahip Karsinoembryonik antijen (CEA) ve Eritrosit sedimentasyon hızına (ESR) sahip olgu sunulacaktır. Preoperatif metastazı olmayan hastaya uygulanan radikal nefroüreterektomi operasyonu sonrası, histolojik tanı yüksek grade’li renal pelvis ürotelyal karsinomu olarak rapor edilmiştir. Cerrahi sınırlar negatif olup serum CEA ve ESR düzeyinde postoperatif erken dönemde tamamen düzelme görülmüştür.Anahtar Kelimeler: Karsinoembriyonik antijen, Ürotelyal tümör, Eritrosit Sedimentasyon Hızı, Renal pelvis, Serum belirteçleri
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- 2015
12. Dyke-Davidoff-Masson Sendrom'lu bir çocuk
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YILDIZ, Sema, TÜRKSOY, Özlem, CILIZ, Deniz, SAKMAN, Bülent, and YÜKSEL, Enis
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Dyke-Davidoff-Masson Sendromu,epilepsi - Abstract
Dyke-Davidoff-Masson Sendrom’u (DDMS), ilk olarak Dyke ve arkadaşları tarafından bildirilmiş serebral hemiatrofi, kontralateral hemiparezi ve epilepsi ile karakterize klinik bir antitedir. İlk kez 1933 yılında tanımlanan hastalık, konjenital ve kazanılmış birçok nedene bağlı olarak ortaya çıkabilmektedir. Bu olgu bildirisinde, 10 yaşında, 8 yıl önce bir kez febril konvülsiyon atağı geçirmiş DDMS’lu kız çocuğu tartışılmıştır.
- Published
- 2014
13. Identification of An mtDNA Setpoint Associated with Highest Levels of CD44 Positivity and Chemoresistance in HGC-27 and MKN-45 Gastric Cancer Cell Lines.
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Terzioğlu, Gökhan, Türksoy, Özlem, and Bayrak, Ömer Faruk
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CANCER stem cells , *MITOCHONDRIAL DNA , *IMMUNOGLOBULINS , *DRUG resistance in cancer cells , *STOMACH cancer , *CANCER cell culture , *POLYMERASE chain reaction - Abstract
Objective: Cancer stem cells (CSCs) have important roles in survival and chemoresistance. These cells are commonly recognized with CD44 and CD24 markers. In this study, we aimed to analyze the effects of mtDNA content on cell surface positivity for anti-CD24 and anti-CD44 antibodies and chemoresistance level in AGS, HGC-27 and MKN-45 gastric cancer (GC) cell lines and to determine a setpoint for mtDNA copy for each cell line. Materials and Methods: In this experimental study, we initially decreased mtDNA levels in AGS, HGC-27 and MKN-45 by EtBr treatment. This depletion was confirmed with quantitative polymerase chain reaction (qPCR). Changes in cell surface positivity for anti-CD24 and anti-CD44 antibodies in control and mtDNA-depleted AGS, HGC-27 and MKN-45 were then analyzed with flow cytometry. Changes in chemoresistance (5-FU and cisplatin) were analyzed for all cell lines. The relationship between mtDNA content and cell surface positivity for CD24 and CD44 markers was examined. Results: The highest CD44 positivity was found in HGC-27 and MKN-45 ρlow cells which had 33-40% mtDNA content of control cells, however, CD24 positivity decreased with mtDNA depletion in all cell lines. The highest chemoresistance levels were found in all ρlow cells. mtDNA-recovered (i.e. reverted) HGC-27 and MKN-45 cells partially maintained their increased chemoresistance while reverted AGS cells did not maintain an increased level of chemoresistance. Conclusion: mtDNA depletion triggers chemoresistance in cancer cell lines and is correlated with increase and decrease of CD44 and CD24 positivity respectively in HGC-27 and MKN-45 GC cell lines. A mtDNA content above or below the identified setpoint (33-40% of that in control cells), results in the decrease of CD44 positivity and chemoresistance levels. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
14. Transrektal ultrasonografi eşliğinde uygulanan prostat biyopsisinde ağrıya yönelik risk faktörlerinin değerlendirilmesi
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Tokgöz, Hüsnü, HANCI, VOLKAN, MUNGAN, NECMETTİN AYDIN, EROL, BÜLENT, Şimşek, Ali Rıza, AKDUMAN, BÜLENT, and Türksoy, Özlem
- Published
- 2012
15. Strategies to Limit Radiation Dose in Pediatric Computed Tomography Applications
- Author
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ÇEÇE, Hasan, TÜRKSOY, Özlem, YILDIZ, Sema, and Zonguldak Bülent Ecevit Üniversitesi
- Subjects
bilgisayarlı tomografi,çocukluk çağı,radyasyon ,computed tomography,childhood,radiation ,Genel ve Dahili Tıp - Abstract
Bilgisayarlı tomografi, pediyatrik yaş grubunda kullanımı gün geçtikçe artan bir görüntüleme tekniğidir. Pediatrik yaş grubunun radyasyona duyarlı olması ve radyasyonun kanser riski oluşturmasında kümülatif dozun etkin olması verilen doza dikkat edilmesini gerektirmektedir. Çocuklara erişkin protokollerinin uygulanması, kilo bağımlı değişikliklerin yapılmaması, verilen dozun ölçümünde ve riskin belirlenmesinde standartların belli olmaması, yüksek dozlar için cezai müeyyidenin olmaması önemli sorunlardır. Ancak gerekli durumlarda, -sadece- hedef vücut alanlarının bilgisayarlı tomografi ile değerlendirilmesi, parametrelerin çocuklar için yeniden düzenlenmesi ve her çocuğa özel protokol değişikliklerinin yapılması ile radyasyon dozu önemli ölçüde azaltılabilir. Radyasyonu azaltmada, kullanılan tomografi aletinin teknik parametre değişiklikleri (tüp akımı, kilovolt piki, masa hızı) iyi bilinmeli ve dikkatlice uygulanmalıdır. Anahtar Kelimeler: bilgisayarlı tomografi, çocukluk çağı, radyasyon, SUMMARY, Computed tomography is an increasingly used imaging modality in childhood. Since pediatric population possess increased radiosensitivity and radiation-related cancer risk is related with cumulative dose, the radiation exposure must be carefully established. Use of the same CT settings for adults and children, deficiency of weight adjusted protocols, lack of consensus for the measurement of dose and related risks of radiation, and absence of penalties for higher doses are essential problems. Radiation dose can significantly be decreased with evaluation of -onlytargeted part of the body in appropriate conditions, rearrangement of the scanning parameters for children, and subject based protocol and dose adjustment for each children. The alternations in technical parameters of CT device like tube current, kilovoltage peak, table speed should be well-known and carefully applied for limitation of the radiation dose.
- Published
- 2012
16. Renal transizyonel hücreli karsinom için olası serum belirteçleri: Literatürün gözden geçirilmesi ile birlikte bir olgunun sunumu
- Author
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Tokgöz, Hüsnü, Türksoy, Özlem, Erol, Bülent, and Zonguldak Bülent Ecevit Üniversitesi
- Subjects
Genel ve Dahili Tıp - Abstract
Üriner sistemin üst bölümünün ürotelyal tümörü, relatif olarak nadir görülen malignitelerden biridir. Prostat ve testis kanserlerine özgü serum belirteçleri bilinmesine rağmen, renal ve ürotelyal tümörler için spesifik belirteçlerin tanımlanmasına ihtiyaç vardır. Bu makalede, yüksek preoperatif serum düzeyine sahip Karsinoembryonik antijen (CEA) ve Eritrosit sedimentasyon hızına (ESR) sahip olgu sunulacaktır. Preoperatif metastazı olmayan hastaya uygulanan radikal nefroüreterektomi operasyonu sonrası, histolojik tanı yüksek grade’li renal pelvis ürotelyal karsinomu olarak rapor edilmiştir. Cerrahi sınırlar negatif olup serum CEA ve ESR düzeyinde postoperatif erken dönemde tamamen düzelme görülmüştür., Urothelial tumors of the upper urinary tract are relatively rare malignancies of urinary system. Despite the presence of specific serum parameters for prostate and testicular cancer, new markers need to be identified for renal and urothelial cancers. We report a case of huge renal pelvis tumor with high serum levels of carcinoembryonic antigen (CEA) and erythrocyte sedimentation rate (ESR). No distant metastasis was evident preoperatively. Radical nephroureterectomy was performed and the tumor was histologically diagnosed as high grade urothelial carcinoma of the renal pelvis. Surgical margins were negative and marked decrease in serum level of CEA and normalization of ESR, were noticed immediately after surgery.
- Published
- 2010
17. İntraserebral Kavernoma Bağlı Koreiform Hareket Bozukluğu: Olgu Sunumu
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Özen Barut, Banu, primary, Demiryürek, Bekir Enes, additional, and Türksoy, Özlem, additional
- Published
- 2013
- Full Text
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18. Bilateral invasive papillary carcinoma of the breast
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Elverici, Eda, primary, Barça, Ayşe Nurdan, additional, Türksoy, Özlem, additional, Araz, Levent, additional, and Yüksel, Enis, additional
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- 2007
- Full Text
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19. İntraserebral Kavernoma Bağlı Koreiform Hareket Bozukluğu: Olgu Sunumu.
- Author
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Barut, Banu Özen, Coşkun, Bekir Enes, and Türksoy, Özlem
- Subjects
BLOOD-vessel abnormalities ,CHOREA ,SYMPTOMS ,CAVERNOUS hemangioma ,DISEASE complications ,DIAGNOSIS - Abstract
Copyright of Turkish Journal of Neurology / Turk Noroloji Dergisi is the property of Galenos Yayinevi Tic. LTD. STI and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2013
- Full Text
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20. POSSIBLE SERUM MARKERS FOR RENAL TRANSITIONAL CELL CARCINOMA: REPORT OF A CASE WITH THE REVIEW OF LITERATURE.
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Tokgöz, Hüsnü, Türksoy, Özlem, and Erol, Bülent
- Subjects
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SERUM , *RENAL cell carcinoma , *KIDNEY surgery , *LITERATURE reviews , *TRANSITIONAL cell carcinoma , *DIAGNOSTIC use of tumor markers , *CARCINOEMBRYONIC antigen - Abstract
Urothelial tumors of the upper urinary tract are relatively rare malignancies of urinary system. Despite the presence of specific serum parameters for prostate and testicular cancer, new markers need to be identified for renal and urothelial cancers. We report a case of huge renal pelvis tumor with high serum levels of carcinoembryonic antigen (CEA) and erythrocyte sedimentation rate (ESR). No distant metastasis was evident preoperatively. Radical nephroureterectomy was performed and the tumor was histologically diagnosed as high grade urothelial carcinoma of the renal pelvis. Surgical margins were negative and marked decrease in serum level of CEA and normalization of ESR, were noticed immediately after surgery. [ABSTRACT FROM AUTHOR]
- Published
- 2010
21. Pyruvate supplementation and plasma treated culture surfaces increase the number of immature thymic epithelial cells and their stemness ability in human thymic cell culture.
- Author
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Terzioğlu, Gökhan, Türksoy, Özlem, Akdeniz, Fatma Tuba, Akbulut, Zeynep, and Yanıkkaya Demirel, Gülderen
- Subjects
- *
FLOW cytometry , *EPITHELIAL cell culture , *EPITHELIAL cells , *THYMUS , *PYRUVATES - Abstract
Introduction: Aged population have to struggle with aging associated diseases as an indirect result of immunosenescence, therefore many scientists have concentrated on the regenerative immunology to reboost the immune system in older people. In association with immunosenescence, thymus which is the main site for maturation and selection of T cells, decrease in size and functionality. T cell maturation in thymus, mainly occurs through interaction between immature T cells and thymic epithelial cells (TECs). Determination of ideal conditions for thymic epithelial cells culture is important approach for future regenerative therapies. In our study, we analyzed the effects of method of thymic cell suspension preparation, supplements in culture medium and culture surfaces on TECs in culture. Materials and Methods: Infant thymuses were homogenized with mechanical homogenization or mechanical homogenization plus dispase treatment. Homogenates were passed through 40μm pore size cell strainer. Cells and tissue parts on the cell strainer were recovered with back wash and they were used for culture. Cells and small tissue fragments (1 mm) from thymus were seeded together in plasma treated polystrene or poly-L lysine coated culture flasks. Cell were cultured with 10% or %1 FBS and 1% or no pyruvate containing medium. Passaged cells were characterized with flow cytometry. CD45neg CD326+ cells were gated as total TECs. CD45neg CD326+ CD205+ cells were identified as cTECs while CD45neg CD326+ CD205neg cells were accepted as mTECs. HLA-DR was used as maturity marker, negative cells were determined as immature while positive cells were determined as mature. For late passage numbers (>5), cells were seeded with 20 ng/mL BFGF, 20 ng/mL EGF, 1xN2 and B27 supplemented culture medium on agarose coated plate for thymosphere formation analysis. Sphere formation assay were used for stemness analysis in vitro. Results: Proliferation of TECs on culture flasks started from the seeded tissue parts. Mechanical treatment maintain TEC morphology and give the highest ratio of immature TECs for late passages (p=5). Pyruvate help the maintenance of TEC characteristics and viability. Cultured cells (p=5) on plasma treated polystrene surface have the 1.6 fold higher immature TECs ratio than cultured cells on poly-L lysine coated surface. Dispase treated cells did not formed spheres while mechanically treated cells formed large spheres. Discussion: Combination of mechanical treatment, plasma treated surfaces and pyruvate supplementation give the highest immature TEC numbers and stemness. These findings will help for optimized thymic cell culture conditions for the purpose of immune rejuvenation. [ABSTRACT FROM AUTHOR]
- Published
- 2016
22. Effects of gender differences and sex hormones on mouse bone marrow cell populations.
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Terzioğlu, Gökhan, Türksoy, Özlem, Eriş, Esra, Gürleyik, Günay, and Yanıkkaya Demirel, Gülderen
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SEX hormones , *BONE marrow cells , *THYMUS , *PROGENITOR cells , *LETROZOLE - Abstract
Introduction: Thymus and bone marrow are the two critical primary lymphoid organs that lose their capacity during aging. Bone marrow (BM) produce the T cell progenitors and they migrate to thymus for selection and maturation. Therefore, thymic T cell production capacity is affected by changes in bone marrow cell populations. It is known that females have lower thymic involution rates than males. In our study, we analyzed the effects of gender differences, sex hormones on bone marrow stem progenitor cells. Materials and Method: C57BL/6 mice were divided into six experimental group, each group was containing seven mice. Seven days old male and female mice bone marrow, thymus and peripheral blood were collected for first 2 groups. Same samples were collected from other groups at the end of the three months. Other groups were fed for 3 months, one male and one female group were fed with normal chow and untreated, while a female group were treated with testosterone and another female group were treated with letrozole for inhibition of estrogen synthesis. Filtered bone marrow was analyzed with flow cytometry for Lin-/lowSca1+CD117+CD135-haematopoietic stem cells (HSC), Lin-/low CD117 high CD135+Sca1+CD90- multipotent progenitor (MPP) cells and Lin-Scalow CD117 low CD127+CD135+ common lymphoid progenitor (CLP) cells. Results: Ratio of MPP and CLP cells in the bone marrow were higher for 7 days and 3 months old females when compared to same age males. MPP cells in the 3 months old female group was 1,5 fold higher than those of 3 months old male group, CLP cells in the 3 months old females was three fold higher than those of 3 months old male group. Letrozole treatment in 3 months old female group decreased the ratio of MPP and CLP cells in total bone marrow cells, ratio of MPP and CLP cells in BM cells significantly increased in testosterone treated 3 months old female group. Discussion: Testosterone treatment in female mice, increased the number of MPP and CLP cells in bone marrow while suppression of estrogen synthesis decreased the number of same cells. Therefore, testosterone is not responsible of the lower numbers of MPP and CLP cells in bone marrow of 3 months old males, estrogen deficiency seems as the main actor in this situation. [ABSTRACT FROM AUTHOR]
- Published
- 2016
23. Pediatrik Yaşta Bilgisayarlı Tomografi Uygulamalarında Radyasyon Dozunu Azaltma Stratejileri.
- Author
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YILDIZ, Sema, ÇEÇE, Hasan, and TÜRKSOY, Özlem
- Subjects
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TOMOGRAPHY , *RADIATION doses , *RADIATION protection , *STRATEGIC planning , *CHILDREN - Abstract
Computed tomography is an increasingly used imaging modality in childhood. Since pediatric population possess increased radiosensitivity and radiation-related cancer risk is related with cumulative dose, the radiation exposure must be carefully established. Use of the same CT settings for adults and children, deficiency of weight adjusted protocols, lack of consensus for the measurement of dose and related risks of radiation, and absence of penalties for higher doses are essential problems. Radiation dose can significantly be decreased with evaluation of -only-targeted part of the body in appropriate conditions, rearrangement of the scanning parameters for children, and subject based protocol and dose adjustment for each children. The alternations in technical parameters of CT device like tube current, kilovoltage peak, table speed should be well-known and carefully applied for limitation of the radiation dose. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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