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590 results on '"Takashima, Yasuhiro"'

1. Far-East Asian Toxoplasma isolates share ancestry with North and South/Central American recombinant lineages

Author

Ihara, Fumiaki, Kyan, Hisako, Takashima, Yasuhiro, Ono, Fumiko, Hayashi, Kei, Matsuo, Tomohide, Igarashi, Makoto, Nishikawa, Yoshifumi, Hikosaka, Kenji, Sakamoto, Hirokazu, Nakamura, Shota, Motooka, Daisuke, Yamauchi, Kiyoshi, Ichikawa-Seki, Madoka, Fukumoto, Shinya, Sasaki, Motoki, Ikadai, Hiromi, Kusakisako, Kodai, Ohari, Yuma, Yoshida, Ayako, Sasai, Miwa, Grigg, Michael E., and Yamamoto, Masahiro

Published
2024
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3. First Report of Mermithidae (Enoplea: Mermithida) Parasitizing Adult Stable Flies in Japan

Author

Shimizu Kaori, Saito Taizo, Takashima Yasuhiro, Okada Haruhiko, Asakawa Mitsuhiko, and Inoshima Yasuo

Subjects
blood-feeding pests, genetics, host–parasite relationship, insect parasitism, mermithidae, stable fly, Biology (General), QH301-705.5
Abstract

Mermithidae is a family of nematodes that parasitize a wide range of invertebrates worldwide. Herein, we report nematodes that were unexpectedly found in three of 486 adult stable flies (Stomoxys calcitrans) captured from three farms (F1, F2, and F3) in different regions of Gifu Prefecture, Japan. We aimed to characterize these nematodes both at the morphological and molecular level. Morphological studies revealed that the nematodes were juveniles of Mermithidae. Phylogenetic analysis based on 18S and 28S rDNA indicated that the mermithids from farms F1 and F2 could be categorized into the same cluster as Ovomermis sinensis and Hexamermis sp., whereas the mermithid from farm F3 clustered with Amphimermis sp. Additionally, these mermithids could be categorized within the same clusters as related mermithids detected in Japan that parasitize various arthropod orders. Our findings suggest that these stable flies may have been parasitized by mermithids already present in the region and that genetically distinct species of mermithids occur across Japan. To the best of our knowledge, this is the first report of mermithids parasitizing adult stable flies in Japan.

Published
2024
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4. H1FOO-DD promotes efficiency and uniformity in reprogramming to naive pluripotency

Author

Kunitomi, Akira, Hirohata, Ryoko, Osawa, Mitsujiro, Washizu, Kaho, Arreola, Vanessa, Saiki, Norikazu, Kato, Tomoaki M., Nomura, Masaki, Kunitomi, Haruko, Ohkame, Tokiko, Ohkame, Yusuke, Kawaguchi, Jitsutaro, Hara, Hiroto, Kusano, Kohji, Yamamoto, Takuya, Takashima, Yasuhiro, Tohyama, Shugo, Yuasa, Shinsuke, Fukuda, Keiichi, Takasu, Naoko, and Yamanaka, Shinya

Published
2024
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8. The pluripotent stem cell-specific transcript ESRG is dispensable for human pluripotency

Author

Takahashi, Kazutoshi, Nakamura, Michiko, Okubo, Chikako, Kliesmete, Zane, Ohnuki, Mari, Narita, Megumi, Watanabe, Akira, Ueda, Mai, Takashima, Yasuhiro, Hellmann, Ines, and Yamanaka, Shinya

Subjects
Biological Sciences, Bioinformatics and Computational Biology, Genetics, Stem Cell Research, Stem Cell Research - Embryonic - Human, Stem Cell Research - Nonembryonic - Human, Regenerative Medicine, Stem Cell Research - Induced Pluripotent Stem Cell, Human Genome, Stem Cell Research - Induced Pluripotent Stem Cell - Human, 2.1 Biological and endogenous factors, Underpinning research, Aetiology, 1.1 Normal biological development and functioning, Generic health relevance, Cell Differentiation, Cells, Cultured, Cellular Reprogramming, Female, Gene Silencing, Humans, Kruppel-Like Factor 4, Neural Stem Cells, Pluripotent Stem Cells, RNA, Long Noncoding, Developmental Biology
Abstract

Human pluripotent stem cells (PSCs) express human endogenous retrovirus type-H (HERV-H), which exists as more than a thousand copies on the human genome and frequently produces chimeric transcripts as long-non-coding RNAs (lncRNAs) fused with downstream neighbor genes. Previous studies showed that HERV-H expression is required for the maintenance of PSC identity, and aberrant HERV-H expression attenuates neural differentiation potentials, however, little is known about the actual of function of HERV-H. In this study, we focused on ESRG, which is known as a PSC-related HERV-H-driven lncRNA. The global transcriptome data of various tissues and cell lines and quantitative expression analysis of PSCs showed that ESRG expression is much higher than other HERV-Hs and tightly silenced after differentiation. However, the loss of function by the complete excision of the entire ESRG gene body using a CRISPR/Cas9 platform revealed that ESRG is dispensable for the maintenance of the primed and naïve pluripotent states. The loss of ESRG hardly affected the global gene expression of PSCs or the differentiation potential toward trilineage. Differentiated cells derived from ESRG-deficient PSCs retained the potential to be reprogrammed into induced PSCs (iPSCs) by the forced expression of OCT3/4, SOX2, and KLF4. In conclusion, ESRG is dispensable for the maintenance and recapturing of human pluripotency.

Published
2021

10. Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis

Author

Huang, Miller, Tailor, Jignesh, Zhen, Qiqi, Gillmor, Aaron H, Miller, Matthew L, Weishaupt, Holger, Chen, Justin, Zheng, Tina, Nash, Emily K, McHenry, Lauren K, An, Zhenyi, Ye, Fubaiyang, Takashima, Yasuhiro, Clarke, James, Ayetey, Harold, Cavalli, Florence MG, Luu, Betty, Moriarity, Branden S, Ilkhanizadeh, Shirin, Chavez, Lukas, Yu, Chunying, Kurian, Kathreena M, Magnaldo, Thierry, Sevenet, Nicolas, Koch, Philipp, Pollard, Steven M, Dirks, Peter, Snyder, Michael P, Largaespada, David A, Cho, Yoon Jae, Phillips, Joanna J, Swartling, Fredrik J, Morrissy, A Sorana, Kool, Marcel, Pfister, Stefan M, Taylor, Michael D, Smith, Austin, and Weiss, William A

Subjects
Medical Biotechnology, Biomedical and Clinical Sciences, Oncology and Carcinogenesis, Pediatric Cancer, Genetics, Transplantation, Brain Disorders, Pediatric, Regenerative Medicine, Brain Cancer, Stem Cell Research - Nonembryonic - Human, Cancer, Neurosciences, Human Genome, Stem Cell Research, Stem Cell Research - Nonembryonic - Non-Human, Rare Diseases, Biotechnology, 2.1 Biological and endogenous factors, Animals, Basal Cell Nevus Syndrome, Brain Neoplasms, Carcinogenesis, DEAD-box RNA Helicases, Disease Models, Animal, Genetic Engineering, Genetic Predisposition to Disease, Humans, Medulloblastoma, Mice, Mice, SCID, N-Myc Proto-Oncogene Protein, Neoplasm Proteins, Neural Stem Cells, Neuroepithelial Cells, Patched-1 Receptor, Pluripotent Stem Cells, Stem Cell Transplantation, Transplantation, Heterologous, SHH, human pluripotent stem cells, medulloblastoma, neuroepithelial stem cells, Biological Sciences, Medical and Health Sciences, Developmental Biology, Biological sciences, Biomedical and clinical sciences
Abstract

Human neural stem cell cultures provide progenitor cells that are potential cells of origin for brain cancers. However, the extent to which genetic predisposition to tumor formation can be faithfully captured in stem cell lines is uncertain. Here, we evaluated neuroepithelial stem (NES) cells, representative of cerebellar progenitors. We transduced NES cells with MYCN, observing medulloblastoma upon orthotopic implantation in mice. Significantly, transcriptomes and patterns of DNA methylation from xenograft tumors were globally more representative of human medulloblastoma compared to a MYCN-driven genetically engineered mouse model. Orthotopic transplantation of NES cells generated from Gorlin syndrome patients, who are predisposed to medulloblastoma due to germline-mutated PTCH1, also generated medulloblastoma. We engineered candidate cooperating mutations in Gorlin NES cells, with mutation of DDX3X or loss of GSE1 both accelerating tumorigenesis. These findings demonstrate that human NES cells provide a potent experimental resource for dissecting genetic causation in medulloblastoma.

Published
2019

13. Reduced MEK inhibition preserves genomic stability in naive human embryonic stem cells

Author

Di Stefano, Bruno, Ueda, Mai, Sabri, Shan, Brumbaugh, Justin, Huebner, Aaron J, Sahakyan, Anna, Clement, Kendell, Clowers, Katie J, Erickson, Alison R, Shioda, Keiko, Gygi, Steven P, Gu, Hongcang, Shioda, Toshi, Meissner, Alexander, Takashima, Yasuhiro, Plath, Kathrin, and Hochedlinger, Konrad

Subjects
Biological Sciences, Stem Cell Research, Regenerative Medicine, Stem Cell Research - Embryonic - Human, Genetics, 1.1 Normal biological development and functioning, DNA Methylation, Embryonic Stem Cells, Genomic Instability, Humans, MAP Kinase Kinase Kinases, Protein Kinase Inhibitors, Proteome, Transcriptome, Technology, Medical and Health Sciences, Developmental Biology, Biological sciences
Abstract

Human embryonic stem cells (hESCs) can be captured in a primed state in which they resemble the postimplantation epiblast, or in a naive state where they resemble the preimplantation epiblast. Naive-cell-specific culture conditions allow the study of preimplantation development ex vivo but reportedly lead to chromosomal abnormalities, which compromises their utility in research and potential therapeutic applications. Although MEK inhibition is essential for the naive state, here we show that reduced MEK inhibition facilitated the establishment and maintenance of naive hESCs that retained naive-cell-specific features, including global DNA hypomethylation, HERVK expression, and two active X chromosomes. We further show that hESCs cultured under these modified conditions proliferated more rapidly; accrued fewer chromosomal abnormalities; and displayed changes in the phosphorylation levels of MAPK components, regulators of DNA damage/repair, and cell cycle. We thus provide a simple modification to current methods that can enable robust growth and reduced genomic instability in naive hESCs.

Published
2018

25. Shear stress in the intervillous space promotes syncytial formation of iPS cells-derived trophoblasts

Author

Inohaya, Asako, Chigusa, Yoshitsugu, Takakura, Masahito, Io, Shingo, Kim, Min-A, Matsuzaka, Yu, Yasuda, Eriko, Ueda, Yusuke, Kawamura, Yosuke, Takamatsu, Shiro, Mogami, Haruta, Takashima, Yasuhiro, Mandai, Masaki, Kondoh, Eiji, Inohaya, Asako, Chigusa, Yoshitsugu, Takakura, Masahito, Io, Shingo, Kim, Min-A, Matsuzaka, Yu, Yasuda, Eriko, Ueda, Yusuke, Kawamura, Yosuke, Takamatsu, Shiro, Mogami, Haruta, Takashima, Yasuhiro, Mandai, Masaki, and Kondoh, Eiji

Abstract

The intervillous space of human placenta is filled with maternal blood, and villous trophoblasts are constantly exposed to the shear stress generated by maternal blood pressure and flow throughout the entire gestation period. However, the effects of shear stress on villous trophoblasts and their biological significance remain unknown. Here, using our recently established naïve human pluripotent stem cells-derived cytotrophoblast stem cells (nCTs) and a device that can apply arbitrary shear stress to cells, we investigated the impact of shear stress on early-stage trophoblasts. After 72 h of exposure to 10 dyn/cm² shear stress, nCTs became fused and multinuclear, and mRNA expression of the syncytiotrophoblast (ST) markers, such as glial cell missing 1, endogenous retrovirus group W member 1 envelope, chorionic gonadotropin subunit beta 3, syndecan 1, pregnancy specific beta-1-glycoprotein 3, placental growth factor, and solute carrier family 2 member 1 were significantly upregulated compared to static conditions. Immunohistochemistry showed that shear stress increased fusion index, human chorionic gonadotropin secretion, and human placental lactogen secretion. Increased microvilli formation on the surface of nCTs under flow conditions was detected using scanning electron microscopy. Intracellular cyclic adenosine monophosphate significantly increased under flow conditions. Moreover, transcriptome analysis of nCTs subjected to shear stress revealed that shear stress upregulated ST-specific genes and downregulated CT-specific genes. Collectively, these findings indicate that shear stress promotes the differentiation of nCTs into ST.

Published
2024

32. Hypoblast from human pluripotent stem cells regulates epiblast development

Author

Okubo, Takumi, primary, Rivron, Nicolas, additional, Kabata, Mio, additional, Masaki, Hideki, additional, Kishimoto, Keiko, additional, Semi, Katsunori, additional, Nakajima-Koyama, May, additional, Kunitomi, Haruko, additional, Kaswandy, Belinda, additional, Sato, Hideyuki, additional, Nakauchi, Hiromitsu, additional, Woltjen, Knut, additional, Saitou, Mitinori, additional, Sasaki, Erika, additional, Yamamoto, Takuya, additional, and Takashima, Yasuhiro, additional

Published
2023
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45. A multi-kingdom genetic barcoding system for precise target clone isolation

Author

Ishiguro, Soh, primary, Ishida, Kana, additional, Sakata, Rina C., additional, Mori, Hideto, additional, Takana, Mamoru, additional, King, Samuel, additional, Bashth, Omar, additional, Ichiraku, Minori, additional, Masuyama, Nanami, additional, Takimoto, Ren, additional, Kijima, Yusuke, additional, Adel, Arman, additional, Toyoshima, Hiromi, additional, Seki, Motoaki, additional, Oh, Ju Hee, additional, Archambault, Anne-Sophie, additional, Nishida, Keiji, additional, Kondo, Akihiko, additional, Kuhara, Satoru, additional, Aburatani, Hiroyuki, additional, Klein Geltink, Ramon I., additional, Takashima, Yasuhiro, additional, Shakiba, Nika, additional, and Yachie, Nozomu, additional

Published
2023
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48. Improved Sendai viral system for reprogramming to naive pluripotency

Author

Kunitomi, Akira, primary, Hirohata, Ryoko, additional, Arreola, Vanessa, additional, Osawa, Mitsujiro, additional, Kato, Tomoaki M., additional, Nomura, Masaki, additional, Kawaguchi, Jitsutaro, additional, Hara, Hiroto, additional, Kusano, Kohji, additional, Takashima, Yasuhiro, additional, Takahashi, Kazutoshi, additional, Fukuda, Keiichi, additional, Takasu, Naoko, additional, and Yamanaka, Shinya, additional

Published
2022
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