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528 results on '"Takeda, Naoki"'

9. Loss of Dja2 accompanies pH deviation in lysosomes and lysosome‐related organelles.

Author

Terada, Kazutoyo, Endo, Motoyoshi, Kiyonari, Hiroshi, Takeda, Naoki, and Oike, Yuichi

Subjects
ORGANELLES, ALVEOLAR macrophages, PROTEIN C, LYSOSOMES, MECONIUM aspiration syndrome, GLYCOLIPIDS, AMILORIDE
Abstract

The Dja2 knockout (Dja2−/−) mice had respiratory distress, and >60% died within 2 days after birth. The surviving adult Dja2−/− mice were infertile and the lungs of Dja2−/− mice showed several abnormalities, including the processing defect of prosurfactant protein C in the alveolar epithelial type II cells and the accumulation of glycolipids in enlarged alveolar macrophages. The luminal pH of acidic organelles in Dja2−/− cells was shifted to pH 5.37–5.45. This deviated pH was immediately restored to control levels (pH 4.56–4.65) by the addition of a diuretic, ethyl isopropyl amiloride (EIPA). Although the role of DJA2 in maintaining the pH homeostasis of lysosome‐related organelles is currently obscure, this rapid and remarkable pH resilience is best explained by an EIPA‐sensitive proton efflux machinery that is disorganized and overactivated due to the loss of Dja2. [ABSTRACT FROM AUTHOR]

Published
2024
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28. Meikin is a conserved regulator of meiosis-I-specific kinetochore function

Author

Kim, Jihye, Ishiguro, Kei-ichiro, Nambu, Aya, Akiyoshi, Bungo, Yokobayashi, Shihori, Kagami, Ayano, Ishiguro, Tadashi, Pendas, Alberto M., Takeda, Naoki, Sakakibara, Yogo, Kitajima, Tomoya S., Tanno, Yuji, Sakuno, Takeshi, and Watanabe, Yoshinori

Subjects
DNA replication -- Analysis, Mitosis -- Analysis, Chromosomes -- Analysis, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

The kinetochore is the crucial apparatus regulating chromosome segregation in mitosis and meiosis. Particularly in meiosis I, unlike in mitosis, sister kinetochores are captured by microtubules emanating from the same spindle pole (mono-orientation) and centromeric cohesion mediated by cohesin is protected in the following anaphase. Although meiotic kinetochore factors have been identified only in budding and fission yeasts, these molecules and their functions are thought to have diverged earlier. Therefore, a conserved mechanism for meiotic kinetochore regulation remains elusive. Here we have identified in mouse a meiosis-specific kinetochore factor that we termed MEIKIN, which functions in meiosis I but not in meiosis II or mitosis. MEIKIN plays a crucial role in both mono-orientation and centromeric cohesion protection, partly by stabilizing the localization of the cohesin protector shugoshin. These functions are mediated mainly by the activity of Polo-like kinase PLK1, which is enriched to kinetochores in a MEIKIN-dependent manner. Our integrative analysis indicates that the long-awaited key regulator of meiotic kinetochore function is Meikin, which is conserved from yeasts to humans., In mitosis, the establishment of sister chromatid cohesion is dependent on cohesin in S phase and maintained until metaphase when the sister chromatids are captured by spindle microtubules from opposite [...]

Published
2015
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