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PURPOSE: To detect the frequency of TNF alpha gene in patients of type 2 diabetes mellitus with chronic periodontitis, periodontitis without any systemic diseases anti healthy controls. METHODS: The case series were consisted of 112 patients with moderate, severe type 2 diabetes mellitus with chronic periodontitis, 99 patients with moderate, severe periodontitis without any systemic disease, 50 age- and gender-matched subjects with health)' periodontal conditions were enrolled. Clinical parameters were measured and recorded including probing depth (PD), clinical attachment loss(CAL), bleeding index(BI), and tooth movement(TM). The polymorphism of TNF-α-308 genotype (TNFl/2)was examined after electrophoresis on agarose gel and ethidium bromide staining. The difference between the case and healthy groups was analysed by Chi-square test, the difference in clinical index among groups which had different allele was analyzed for ANOVA with SPSSI3.0 software package. RESULTS: We divided DM and CP groups into moderate and severe groups. There were significant difference between severe DM group and severe, moderate CP group, moderate DM group and chronic periodontitis of severe moderate group. The probing depth and clinical attachment loss of the patients who took TNF-α-308 allele II were significantly higher than the patients who took TNF-α-308 allele I in DM and CP group. CONCLUSIONS: TNF-α-308 allele II might increase the susceptivity of periodontitis in population. TNF-α-308 allele II may play an important role in synergistic reaction of periodontitis and type 2 diabetes. Supported by Project of National Science & Technology Pillar Program during the "Eleventh Five-year" Plan Period (2007BA18B02). [ABSTRACT FROM AUTHOR]

Objective: To investigate the type 2 diabetic patient's periodontal condition and to analyze the influencing factors of periodontitis., Methods: A total of 182 type 2 diabetic patients were included in the survey and requested to fill out a questionnaire, and their periodontal status was evaluated by measuring probing depth (PD), attachment level (AL), sulcus bleeding index (SBI), simplified oral hygiene index (OHI-S)., Results: The prevalence of periodontitis in this group of patients was 96.7% (176/182), including 20 patients with mild periodontitis, 156 with moderate to advanced periodontitis. The mean PD and AL of the 182 patients were (2.92 +/- 0.67) mm and (2.87 +/- 1.31) mm. At least one tooth was lost in 57.1% (104/182) of the patients. The factors related to periodontitis were age, gender, smoking, living in town or country, and 2 h plasma glucose of oral glucose tolerance test (OGTT). There was no relationship between the severity of periodontitis and education level. The majority of patients did not receive any periodontal therapy., Conclusions: Periodontal status was bad in patients with type 2 diabetes. It is important to develop an education program on oral health for type 2 diabetic patients.

Purpose: To evaluate the effectiveness of combined use of rh-bFGF and Bio-Oss collagen compounds on regeneration of the canine periodontal tissues., Methods: Four adult hybrid canine were selected and bone defect of 5mmx5mmx5mm in size was created in the mesio and distal alveolar crest where the premolars were lost in both sides of the maxilla and mandible. Three methods were used in 3 groups,in the experimental group 1, rh-bFGF and Bio-Oss collagen compounds were implanted,Bio-Gide were overlaid; in the experimental group 2,Bio-Oss collagen compounds were implanted, Bio-Gide were overlaid;in the control group only Bio-Gide were overlaid. The therapeutic results were evaluated by clinical and imaging examination as well as histological index. SAS 6.2 software package was used for paired t test., Results: 8 weeks post operation, there were various amounts of newly formed alveolar bone,cement and connective tissues in each 3-wall intrabony defect. There was significant difference in alveolar osteogenesis between the control group(1.2mm+/-0.1mm) and the 2 experimental groups (3.7mm+/-0.3mm, 2.3mm+/-0.2mm).The difference was also significant between the two experimental groups (P<0.01). There was no significant difference in newly formed cement and connective tissues among the 3 groups (P>0.05)., Conclusions: Application of rh-bFGF/ Bio-Oss collagen compounds benefits to the regeneration of the alveolar bone and avoid the side effects of root resorption ,bone malunion which often occur after conventional periodontal regeneration therapy.Addition of rh-bFGF/ Bio-Oss collagen compounds was useless to cement genesis and periodontal ligament formation.

Purpose: Using the MSCs-Bio-Oss tissue engineered bone which was constructed by MSCs as seed cells and the Bio-Oss calf inorganic bone grains as scaffold materials to determine the canine bone formation activity and the feasibility of Bio-Oss combined MSCs to construct tissue engineered bone., Methods: Gybrid canine MSCs were dissociated, cultivated, bone formation induced and differentiated into osteoblast in vitro; The bone formation induced MSCs were allowed to grow onto Bio-Oss calf inorganic bone grains at 10(6) cell/ml, and then incubated and cultivated, under light pressure without other treatments. Inverted phase contrast microscope and scanning electron microscope were used to observe their morphological changes, immunofluorescent labeling of cell surface factor CD44,calcium nodus staining,qualitative and quantitative detection of ALP were carried out, and SPSS 12.0 software package was used for statistical analysis., Results: The MSCs were uniform with compact alignment and shape of prosenchymatous cells, CD44's surface antigen was positive; Osteoblast activity was present after induction and differentiation, alizarin Bordeaux S stain of calcium nodus was positive, ALP's Gomori staining was also positive. ALP content in the experimental group were (3.307 +/- 0.217) U/g, (5.929 +/- 0.781) U/g and (9.739 +/- 0.547)U/g respectively at 3rd day, 7th day, 14th day after induction and differentiation, which were significantly different (P < 0.01) from the control group: (0.442 +/- 0.087) U/g, (0.581 +/- 0.027)U/g and (0.768 +/- 0.126) U/g; the MSCs stuck compactly on the surface of Bio-Oss, grew well, and formed tissue engineered bone., Conclusion: Using canine MSCs which were induced by bone formation as seed cells combined with Bio-Oss as scaffold materials to construct tissue engineered bone is feasible. Supported by Liaoning Provincial Natural Science