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As a material carrier contributing to human survival and social sustainable development, the ecological environment is declining in its integrity and overall health. With the rapid development of society and economy, it is currently very necessary to carry out ecological security evaluation research to provide scientific guidance and suggestions for the construction of ecological civilization and the harmonious co-existence between man and nature. Taking Altay region as the research area, this paper collected and integrated regional geological, geographical, cultural, socio-economic, and statistical data, as well as previous research results. Combined with DPSIR and EES framework model, the evaluation index system of land resource ecological security in Altay region was constructed by using the analytic hierarchy process, entropy method and linear weighted summation function method. Using this index system, the evaluation research work was carried out to determine the current state of the security situation and the major threats which should be addressed. (1) The overall ecological security situation of Altay region was relatively safe, while the local ecological security situation was relatively fragile. Among them, the areas with safe and safer ecological environment accounted for 38.72%, while the areas with critically safe status accounted for 30.83%, and the areas with a less safe and unsafe environment accounted for 30.45%. In terms of spatial characteristics, the areas with unsafe ecological environment were mainly distributed in the west and east of the study area, while the areas with good ecological environment were distributed in the north of the study area. (2) Large-scale mining activities, frequent geological disasters, large-scale reclamation and long-term cultivation of arable land, and long-term large-scale grazing activities resulting in the destruction of grassland and vegetation were the main factors leading to the prominent ecological security problems of land resources in the Altay region. Therefore, in the process of the continuous development of the urban economy, we should pay more attention to the harmony between man and nature, and also actively and effectively advocate and implement certain policies and measures, such as returning farmland to forest, returning grazing land to grassland and integrating the mining of mineral resources.