Tang Chi-Yan., Thesis (M.Phil.)--Chinese University of Hong Kong, 2005., Includes bibliographical references (leaves 89-112)., s in English and Chinese., p.i, Acknowledgements --- p.vi, Table of contents --- p.viii, List of Tables --- p.xi, List of Figures --- p.xii, List of Abbreviations --- p.xiv, Chapter Chapter 1. --- General introduction --- p.1, Chapter Chapter 2. --- Literature review --- p.4, Chapter 2.1 --- History and prevalence of food allergy --- p.4, Chapter 2.2 --- Mechanism and clinical symptoms of food allergy --- p.6, Chapter 2.3 --- Tropomyosin as a major shellfish allergen --- p.13, Chapter 2.4 --- Use of animal model in the studies of food allergy --- p.22, Chapter 2.5 --- Future approaches for treatment of food allergy --- p.27, Chapter Chapter 3. --- Cloning and expression of recombinant tropomyosin --- p.30, Chapter 3.1 --- Introduction --- p.30, Chapter 3.2 --- Materials and Methods --- p.31, Chapter 3.2.1 --- Design of PCR primers for amplification of tropomyosin gene --- p.31, Chapter 3.2.2 --- Cloning of PCR-amplified cDNA into vector --- p.32, Chapter 3.2.3 --- Transformation of competent E. coli Ml5 cells --- p.34, Chapter 3.2.4 --- Confirmation of DNA sequence of the cloned vector --- p.34, Chapter 3.2.5 --- Induction of the recombinant protein --- p.35, Chapter 3.2.6 --- Purification and storage of the recombinant protein under native condition --- p.36, Chapter 3.2.7 --- Concentration measurement and storage of the recombinant protein --- p.37, Chapter 3.2.8 --- Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) --- p.38, Chapter 3.2.9 --- Regeneration of the Ni-NTA column --- p.40, Chapter 3.3 --- Results and discussion --- p.42, Chapter 3.3.1 --- DNA sequence of the cloned vector --- p.42, Chapter 3.3.2 --- Expression of the recombinant protein --- p.42, Chapter 3.3.3 --- Sodium dodecyl sulfate polyacrylamide gel eletrophoresis (SDS-PAGE) --- p.43, Chapter Chapter 4. --- Induction of hypersensitive response to shrimp tropomyosin in mice --- p.47, Chapter 4.1 --- Introduction --- p.47, Chapter 4.2 --- Materials and methods --- p.52, Chapter 4.2.1 --- Mice and reagents --- p.52, Chapter 4.2.2 --- Animal sensitization and challenge --- p.53, Chapter 4.2.3 --- Morphological and behavioral changes --- p.54, Chapter 4.2.4 --- Tropomyosin-specific IgE level --- p.55, Chapter 4.2.5 --- Passive cutaneous anaphylaxis (PCA) reaction --- p.56, Chapter 4.2.6 --- Tropomyosin-specific cellular proliferation level of splenocytes --- p.56, Chapter 4.2.7 --- Cytokine profiles of splenoctyes --- p.58, Chapter 4.2.8 --- Histological examination of small intestine --- p.59, Chapter 4.2.9 --- Statistical analysis --- p.59, Chapter 4.3 --- Results --- p.63, Chapter 4.3.1 --- Morphological and behavioral changes after challenge --- p.63, Chapter 4.3.2 --- Tropomyosin-specific IgE level --- p.63, Chapter 4.3.3 --- Passive cutaneous anaphylaxis (PCA) --- p.64, Chapter 4.3.4 --- Tropomyosin-specific cellular proliferation level of splenocytes --- p.68, Chapter 4.3.5 --- Cytokine profiles of splenocytes --- p.70, Chapter 4.3.6 --- Histology of small intestines --- p.76, Chapter 4.4 --- Discussion --- p.79, Chapter Chapter 5. --- General conclusion --- p.88, References --- p.89, http://library.cuhk.edu.hk/record=b5892545, Use of this resource is governed by the terms and conditions of the Creative Commons “Attribution-NonCommercial-NoDerivatives 4.0 International” License (http://creativecommons.org/licenses/by-nc-nd/4.0/)