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2. Pulsed electromagnetic fields reduce acute inflammation in the injured rat-tail intervertebral disc.

Author

Chan, Andrew K, Tang, Xinyan, Mummaneni, Nikhil V, Coughlin, Dezba, Liebenberg, Ellen, Ouyang, Annie, Dudli, Stefan, Lauricella, Michael, Zhang, Nianli, Waldorff, Erik I, Ryaby, James T, and Lotz, Jeffrey C

Subjects
degeneration model, inflammation, needle stab, pulsed electromagnetic fields, rat‐tail disc
Abstract

Pro-inflammatory cytokines are recognized contributors to intervertebral disc (IVD) degeneration and discogenic pain. We have recently reported the anti-inflammatory effect of pulsed electromagnetic fields (PEMF) on IVD cells in vitro. Whether these potentially therapeutic effects are sufficiently potent to influence disc health in vivo has not been demonstrated. We report here the effect of PEMF on acute inflammation arising from a rat-tail IVD injury model. Disc degeneration was induced by percutaneously stabbing the Co6-7, Co7-8, and Co8-9 levels using a 20-gauge needle. Seventy-two (72) rats were divided into three groups: sham control, needle stab, needle stab+PEMF. Treated rats were exposed to PEMF immediately following surgery and for either 4 or 7 days (4 hr/d). Stab and PEMF effects were evaluated by measuring inflammatory cytokine gene expression (RT-PCR) and protein levels (ELISA assay), anabolic and catabolic gene expression (RT-PCR), and histologic changes. We observed in untreated animals that at day 7 after injury, inflammatory cytokines (interleukin [IL]-6, tumor necrosis factor α, and IL-1β) were significantly increased at both gene and protein levels (P

Published
2019

3. Glial state changes and neuroinflammatory RIPK1 signaling are a key feature of ALS pathogenesis

Author

Zelic, Matija, primary, Blazier, Anna, additional, Pontarelli, Fabrizio, additional, LaMorte, Michael, additional, Huang, Jeremy, additional, Tasdemir-Yilmaz, Ozge E., additional, Ren, Yi, additional, Ryan, Sean K., additional, Krishnaswami, Pavithra, additional, Levit, Mikhail, additional, Sood, Disha, additional, Chen, Yao, additional, Gans, Joseph, additional, Tang, Xinyan, additional, Hsiao-Nakamoto, Jennifer, additional, Huang, Fen, additional, Zhang, Bailin, additional, Gaglia, Giorgio, additional, Ofengeim, Dimitry, additional, and Hammond, Timothy R., additional

Published
2024
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4. Abstract B070: Logic-gated CAR T cell product AB-1015 response to ovarian cancer models with heterogeneous levels of ALPG/P antigen

Author

Polyak, Dina, primary, Beckman, Erik, additional, Balakrishnan, Preethi Bala, additional, Yun, Hongruo, additional, Tang, Xinyan, additional, Feng, Jun, additional, Attansio, Nickolas, additional, Cass, Ashley, additional, Santoro, Stephen, additional, Gonzalez-Junca, Alba, additional, Haining, W. Nicholas, additional, and Murriel, Christopher L., additional

Published
2024
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5. Deciphering lung adenocarcinoma prognosis and immunotherapy response through an AI‐driven stemness‐related gene signature.

Author

Ye, Bicheng, Hongting, Ge, Zhuang, Wen, Chen, Cheng, Yi, Shulin, Tang, Xinyan, Jiang, Aimin, and Zhong, Yating

Subjects
MACHINE learning, RNA sequencing, ARTIFICIAL intelligence, DEEP learning, IMMUNE response
Abstract

Lung adenocarcinoma (LUAD) is a leading cause of cancer‐related deaths, and improving prognostic accuracy is vital for personalised treatment approaches, especially in the context of immunotherapy. In this study, we constructed an artificial intelligence (AI)‐driven stemness‐related gene signature (SRS) that deciphered LUAD prognosis and immunotherapy response. CytoTRACE analysis of single‐cell RNA sequencing data identified genes associated with stemness in LUAD epithelial cells. An AI network integrating traditional regression, machine learning, and deep learning algorithms constructed the SRS based on genes associated with stemness. Subsequently, we conducted a comprehensive exploration of the connection between SRS and both intrinsic and extrinsic immune environments using multi‐omics data. Experimental validation through siRNA knockdown in LUAD cell lines, followed by assessments of proliferation, migration, and invasion, confirmed the functional role of CKS1B, a top SRS gene. The SRS demonstrated high precision in predicting LUAD prognosis and likelihood of benefiting from immunotherapy. High‐risk groups classified by the SRS exhibited decreased immunogenicity and reduced immune cell infiltration, indicating challenges for immunotherapy. Conversely, in vitro experiments revealed CKS1B knockdown significantly impaired aggressive cancer phenotypes like proliferation, migration, and invasion of LUAD cells, highlighting its pivotal role. These results underscore a close association between stemness and tumour immunity, offering predictive insights into the immune landscape and immunotherapy responses in LUAD. The newly established SRS holds promise as a valuable tool for selecting LUAD populations likely to benefit from future clinical stratification efforts. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Molecular Determinant Underlying Selective Coupling of Primary G‐Protein by Class A GPCRs.

Author

Shen, Qingya, Tang, Xinyan, Wen, Xin, Cheng, Shizhuo, Xiao, Peng, Zang, Shao‐Kun, Shen, Dan‐Dan, Jiang, Lei, Zheng, Yanrong, Zhang, Huibing, Xu, Haomang, Mao, Chunyou, Zhang, Min, Hu, Weiwei, Sun, Jin‐Peng, Zhang, Yan, and Chen, Zhong

Subjects
*G protein coupled receptors, *AMINO group, *HISTAMINE receptors, *MACHINE learning, *FUNCTIONAL analysis, *HISTAMINE, *G proteins
Abstract

G‐protein‐coupled receptors (GPCRs) transmit downstream signals predominantly via G‐protein pathways. However, the conformational basis of selective coupling of primary G‐protein remains elusive. Histamine receptors H2R and H3R couple with Gs‐ or Gi‐proteins respectively. Here, three cryo‐EM structures of H2R‐Gs and H3R‐Gi complexes are presented at a global resolution of 2.6‐2.7 Å. These structures reveal the unique binding pose for endogenous histamine in H3R, wherein the amino group interacts with E2065.46 of H3R instead of the conserved D1143.32 of other aminergic receptors. Furthermore, comparative analysis of the H2R‐Gs and H3R‐Gi complexes reveals that the structural geometry of TM5/TM6 determines the primary G‐protein selectivity in histamine receptors. Machine learning (ML)‐based structuromic profiling and functional analysis of class A GPCR–G‐protein complexes illustrate that TM5 length, TM5 tilt, and TM6 outward movement are key determinants of the Gs and Gi/o selectivity among the whole Class A family. Collectively, the findings uncover the common structural geometry within class A GPCRs that determines the primary Gs‐ and Gi/o‐coupling selectivity. [ABSTRACT FROM AUTHOR]

Published
2024
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7. Quantification of Propionic Acid in the Bovine Spinal Disk After Infection of the Tissue With Propionibacteria acnes Bacteria

Author

Magnitsky, Sergey, Dudli, Stefan, Tang, Xinyan, Kaur, Jaskanwaljeet, Diaz, Joycelyn, Miller, Steve, and Lotz, Jeffrey C

Subjects
Medical Biochemistry and Metabolomics, Biomedical and Clinical Sciences, Clinical Sciences, Infectious Diseases, Pain Research, Chronic Pain, Biomedical Imaging, Infection, Animals, Back Pain, Biomarkers, Cattle, Gram-Positive Bacterial Infections, Humans, Intervertebral Disc, Magnetic Resonance Imaging, Magnetic Resonance Spectroscopy, Propionates, Propionibacterium acnes, biomarker, intervertebral disk, low back pain, Propionibacteria acnes, Biomedical Engineering, Orthopedics, Clinical sciences, Neurosciences, Allied health and rehabilitation science
Abstract

Study designResearch.ObjectiveThe goal of this study was to investigate whether Propionibacteria acnes infection of the intervertebral disc can be detected noninvasively by nuclear magnetic resonance (NMR) spectroscopy.Summary of background dataMicrobiological studies of surgical samples suggest that a significant subpopulation of back pain patients may have occult disc infection with P. acnes bacteria. This hypothesis is further supported by a double-blind clinical trial showing that back pain patients with Modic type 1 changes may respond to antibiotic treatment. Because significant side effects are associated with antibiotic treatment, there is a need for a noninvasive method to detect whether specific discs in back pain patients are infected with P acnes bacteria.MethodsP. acnes bacteria were obtained from human patients. NMR detection of a propionic acid (PA) in the bacteria extracts was conducted on 500 MHz high-resolution spectrometer, whereas in vivo NMR spectroscopy of an isolated bovine disk tissue infected with P. acnes was conducted on 7 T magnetic resonance imaging scanner.ResultsNMR spectra of P. acnes metabolites revealed a distinct NMR signal with identical chemical shits (1.05 and 2.18 ppm) as PA (a primary P. acne metabolite). The 1.05 ppm signal does not overlap with other bacteria metabolites, and its intensity increases linearly with P. acnes concentration. Bovine disks injected with P. acnes bacteria revealed a very distinct NMR signal at 1.05 ppm, which linearly increased with P. acnes concentration.ConclusionThe 1.05 ppm NMR signal from PA can be used as a marker of P. acnes infection of discs. This signal does not overlap with other disc metabolites and linearly depends on P. acnes concentration. Consequently, NMR spectroscopy may provide a noninvasive method to detect disc infection in the clinical setting.Level of evidenceN/A.

Published
2018

8. Dynamic imaging demonstrates that pulsed electromagnetic fields (PEMF) suppress IL‐6 transcription in bovine nucleus pulposus cells

Author

Tang, Xinyan, Alliston, Tamara, Coughlin, Dezba, Miller, Stephanie, Zhang, Nianli, Waldorff, Erik I, Ryaby, James T, and Lotz, Jeffrey C

Subjects
Engineering, Health Sciences, Sports Science and Exercise, Biomedical Engineering, Aetiology, 2.1 Biological and endogenous factors, Musculoskeletal, Animals, Cattle, Electromagnetic Fields, Genes, Reporter, Interleukin-1alpha, Interleukin-6, Intervertebral Disc Degeneration, Magnetic Field Therapy, Nucleus Pulposus, Primary Cell Culture, Real-Time Polymerase Chain Reaction, Transcription, Genetic, MS2-GFP reporter, dynamic imaging, pulsed electromagnetic fields, IL-6 mRNA expression, spine, disc biology, spine/disc biology, Clinical Sciences, Human Movement and Sports Sciences, Orthopedics, Biomedical engineering, Sports science and exercise
Abstract

Inflammatory cytokines play a dominant role in the pathogenesis of disc degeneration. Pulsed electromagnetic fields (PEMF) are noninvasive biophysical stimulus that has been used extensively in the orthopaedic field for many years. However, the specific cellular responses and mechanisms involved are still unclear. The objective of this study was to assess the time-dependent PEMF effects on pro-inflammatory factor IL-6 expression in disc nucleus pulposus cells using a novel green fluorescence protein (GFP) reporter system. An MS2-tagged GFP reporter system driven by IL-6 promoter was constructed to visualize PEMF treatment effect on IL-6 transcription in single living cells. IL-6-MS2 reporter-labeled cells were treated with IL-1α to mimic the in situ inflammatory environment of degenerative disc while simultaneously exposed to PEMF continuously for 4 h. Time-lapse imaging was recorded using a confocal microscope to track dynamic IL-6 transcription activity that was demonstrated by GFP. Finally, real-time RT-PCR was performed to confirm the imaging data. Live cell imaging demonstrated that pro-inflammatory factor IL-1α significantly promoted IL-6 transcription over time as compared with DMEM basal medium condition. Imaging and PCR data demonstrated that the inductive effect of IL-1α on IL-6 expression could be significantly inhibited by PEMF treatment in a time-dependent manner (early as 2 h of stimulus initiation). Our data suggest that PEMF may have a role in the clinical management of patients with chronic low back pain. Furthermore, this study shows that the MS2-tagged GFP reporter system is a useful tool for visualizing the dynamic events of mechanobiology in musculoskeletal research. © 2017 The Authors. Journal of Orthopaedic Research® Published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society. J Orthop Res 35:778-787, 2018.

Published
2018

10. Data from CD47-blocking Antibody ZL-1201 Promotes Tumor-associated Macrophage Phagocytic Activity and Enhances the Efficacy of the Therapeutic Antibodies and Chemotherapy

Author

Cao, Anthony, primary, Yi, Jiaqing, primary, Tang, Xinyan, primary, Szeto, Christopher W., primary, Wu, Renyi, primary, Wan, Bing, primary, Fang, Xu, primary, Li, Shou, primary, Wang, Lei, primary, Wang, Lina, primary, Li, Jing, primary, Ye, Qiuping, primary, Huang, Tom, primary, Hsu, Karl, primary, Kabbarah, Omar, primary, and Zhou, Haiying, primary

Published
2023
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11. Figure S7 from CD47-blocking Antibody ZL-1201 Promotes Tumor-associated Macrophage Phagocytic Activity and Enhances the Efficacy of the Therapeutic Antibodies and Chemotherapy

Author

Cao, Anthony, primary, Yi, Jiaqing, primary, Tang, Xinyan, primary, Szeto, Christopher W., primary, Wu, Renyi, primary, Wan, Bing, primary, Fang, Xu, primary, Li, Shou, primary, Wang, Lei, primary, Wang, Lina, primary, Li, Jing, primary, Ye, Qiuping, primary, Huang, Tom, primary, Hsu, Karl, primary, Kabbarah, Omar, primary, and Zhou, Haiying, primary

Published
2023
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14. CD47-blocking Antibody ZL-1201 Promotes Tumor-associated Macrophage Phagocytic Activity and Enhances the Efficacy of the Therapeutic Antibodies and Chemotherapy

Author

Cao, Anthony, primary, Yi, Jiaqing, additional, Tang, Xinyan, additional, Szeto, Christopher W., additional, Wu, Renyi, additional, Wan, Bing, additional, Fang, Xu, additional, Li, Shou, additional, Wang, Lei, additional, Wang, Lina, additional, Li, Jing, additional, Ye, Qiuping, additional, Huang, Tom, additional, Hsu, Karl, additional, Kabbarah, Omar, additional, and Zhou, Haiying, additional

Published
2022
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16. Safety, pharmacokinetics and target engagement of novel RIPK1 inhibitor SAR443060 (DNL747) for neurodegenerative disorders: Randomized, placebo‐controlled, double‐blind phase I/Ib studies in healthy subjects and patients

Author

Vissers, Maurits F. J. M., primary, Heuberger, Jules A. A. C., additional, Groeneveld, Geert Jan, additional, Oude Nijhuis, Jerome, additional, De Deyn, Peter Paul, additional, Hadi, Salah, additional, Harris, Jeffrey, additional, Tsai, Richard M., additional, Cruz‐Herranz, Andres, additional, Huang, Fen, additional, Tong, Vincent, additional, Erickson, Rebecca, additional, Zhu, Yuda, additional, Scearce‐Levie, Kimberly, additional, Hsiao‐Nakamoto, Jennifer, additional, Tang, Xinyan, additional, Chang, Megan, additional, Fox, Brian M., additional, Estrada, Anthony A., additional, Pomponio, Robert J., additional, Alonso‐Alonso, Miguel, additional, Zilberstein, Moshe, additional, Atassi, Nazem, additional, Troyer, Matthew D., additional, and Ho, Carole, additional

Published
2022
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17. Using Boundary Element Method to Calculate the Bending Center of General Section

Author

Tang Xinyan

Subjects
Engineering (General). Civil engineering (General), TA1-2040
Abstract

Using Siant — Venant bending theory, transverse bending of a general section cylinder, it comes down to solving two boundary integral equations of the same type, the bending function and additional torsion function of the cylinder are obtained. On this basis, using boundary element method to determine the bending center of general section. Finally, to illustrate the application of the method, a numerical example is given.

Published
2019
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28. Giant magnetoimpedance current sensor with array-structure double probes

Author

Han, Bing, Zhang, Tao, Zhang, Ke, Yao, Bin, Yue, Xinglong, Huang, Dongyan, Ren, Huan, and Tang, Xinyan

Subjects
Amorphous substances -- Electric properties, Impedance (Electricity) -- Control, Business, Electronics, Electronics and electrical industries
Abstract

We have designed a novel giant magnetoimpedance (GMI) noncontact current sensor with array structure double probes. Unlike previously reported sensors, our sensor's probes consist of many pieces of commercial amorphous ribbons, which are parallel to each other, equidistant, and connected in series, and a permanent magnet provides a bias magnetic field. The double-probe output shows the best sensitivity and linearity at a bias magnetic field of 7.40 Oe. Under this field, the sensor shows sensitivity of 1 V/A in the current range of [+ or -] 3 A, measurement precision of less than 0.15 % at room temperature, and good thermal stability in the temperature region between -20[degrees]C and 30[degrees]C. Index Terms--Amorphous ribbon, current sensor, double-probe, giant magnetoimpedance.

Published
2008

32. Longitudinal biomarkers in amyotrophic lateral sclerosis

Author

Huang, Fen, primary, Zhu, Yuda, additional, Hsiao‐Nakamoto, Jennifer, additional, Tang, Xinyan, additional, Dugas, Jason C., additional, Moscovitch‐Lopatin, Miriam, additional, Glass, Jonathan D., additional, Brown, Robert H., additional, Ladha, Shafeeq S., additional, Lacomis, David, additional, Harris, Jeffrey M., additional, Scearce‐Levie, Kimberly, additional, Ho, Carole, additional, Bowser, Robert, additional, and Berry, James D., additional

Published
2020
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40. 5th International Symposium on Focused Ultrasound

Author

Abounader, Roger, Abraham, Christopher, Adema, Gosse, Agrawal, Punit, Airan, Raag, Aleman, Dionne, Alexander, Phillip, Alkins, Ryan, Alnazeer, Moez, Altman, Michael, Aly, Amirah, Amaral, Joao G., Amrahli, Maral, Amraoui, Sana, Andarawewa, Kumari, Andriyakhina, Yulia, Angstadt, Mary, Ankou, Bénédicte, Arias, Ana C., Arvanitis, Costas, Asadnia, Kiana, Aubert, Isabelle, Aubry, Jean-Francois, Aurup, Christian, Bader, Kenneth, Badr, Lena, Baek, Hongchae, Barbato, Gaetano, Beccaria, Kevin, Bellorofonte, Carlo, Benson, Lee, Bernus, Olivier, Berriet, Rémi, Bertolina, Jim, Beskin, Viktoriya, Bessière, Francis, Bethune, Allison, Bezzi, Mario, Bond, Aaron, Bonomo, Guido, Borowsky, Alexander, Borys, Nicolas, Böttcher, Joachim, Bouley, Donna, Bour, Pierre, Bourekas, Eric, Brenin, David, Brokman, Omer, Brosh, Inbar, Buckner, Andrew, Bullock, Timothy, Cafarelli, Andrea, Cahill, Jessica, Camarena, Francisco, Camelo-Piragua, Sandra, Campbell, Benjamin, Campbell, Fiona, Cannata, Jon, Canney, Michael, Carlson, Roy, Carneiro, Antonio, Carpentier, Alexandre, Catheline, Stefan, Cavin, Ian, Cesana, Claudio, Chabok, Hamid R., Chamanara, Marzieh, Chang, Jin H., Chang, Won S., Changizi, Barbara, Chapelon, Jean Y., Chaplin, Vandiver, Chapman, Martin, Chaudhary, Neeraj, Chaussy, Christian, Chen, Cherry, Chen, Johnny, Chen, Wohsing, Chen, Xiaoming, Chevalier, Philippe, Chiou, George, Chisholm, Alexander, Christofferson, Ivy, Chung, Hyun H., Ciuti, Gastone, Clement, Gregory, Cooper, Mark, Corea, Joseph, Corso, Cristiano, Cosman, Josh, Coughlin, Dezba, Crake, Calum, Cunitz, Bryan, Curiel, Laura, Curley, Colleen T., Czarnota, Gregory, Dababou, Susan, Dallapiazza, Robert, de Bever, Joshua, de Jager, Bram, de Ruiter, Joost, de Senneville, Baudouin D., Deckers, Roel, Delattre, Jean-Yves, den Brok, Martijn, Dhanaliwala, Ali, Diodato, Alessandro, Dixon, Adam, Donner, Elizabeth, Downs, Matthew, Du, Zhongmin, Dubois, Rémi, Dupre, Aurelien, Eikelenboom, Dylan, Elias, W. J., Ellens, Nicholas, Endre, Ruby, Eran, Ayelet, Erasmus, Hans-Peter, Everstine, Ashli, Farahani, Keyvan, Farrer, Alexis, Farry, Justin, Federau, Christian, Feng, Xue, Ferrer, Cyril, Ferrera, Vincent, Fishman, Paul, Foley, Jessica, Frenkel, Victor, Fütterer, Jurgen, Gach, H. M., Gandhi, Dheeraj, Gertner, Michael, Goldsher, Dorit, Gorgone, Alessandro, Greillier, Paul, Griesenauer, Rebekah, Grissom, William, Grondin, Julien, Guha, Chandan, Gulati, Amitabh, Gullapalli, Rao, Guo, Sijia, Gupta, Samit, Gurm, Hitinder, Gwinn, Ryder, Hadley, Rock, Haïssaguerre, Michel, Hammoud, Dima, Hananel, Arik, Hargrove, Amelia, Hatch, Robert, Haworth, Kevin, Hazan, Eilon, He, Ye, Heemels, Maurice, Heerschap, Arend, Hilas, Elaine, Hoang-Xuan, Khe, Hocini, Mélèze, Hodaie, Mojgan, Hofmann, Denis, Holland, Christy, Hoogenboom, Martijn, Hopyan, Sevan, Hossack, John, Houdouin, Alexandre, Hsu, Po-Hung, Hu, Jim, Hurwitz, Mark, Huss, Diane, Hwang, Chang-il, Hwang, Joo H., Idbaih, Ahmed, Ikeuchi, Masahiko, Ingham, Elizabeth, Ives, Kimberly, Izumi, Masashi, Jackson-Lewis, Vernice, Janát-Amsbury, Margit, Jang, Kee W., Jedruszczuk, Kathleen, Jiménez-Gambín, Sergio, Jiménez, Noé, Johnson, Sara, Jonathan, Sumeeth, Joy, Joyce, Jung, Hyun H., Jung, Na Y., Kahn, Itamar, Kamimura, Hermes, Kamrava, Seyed K., Kang, Jeeun, Kang, Kook J., Kang, Soo Y., Kao, Yi-tzu, Katti, Prateek, Kawasaki, Motohiro, Kaye, Elena, Keupp, Jochen, Kim, AeRang, Kim, Harry, Kim, Hyun-Chul, Kim, Hyuncheol, Kim, Hyungmin, Kim, Min S., Kim, Namho, Kiyasu, Katsuhito, Kneepkens, Esther, Knopp, Michael, Kobus, Thiele, Koral, Korgun, Kreider, Wayne, Krishna, Vibhor, Krug, Roland, Krupa, Steve, Kuo, Chia-Chun, Kwiecinski, Wojciech, Lacoste, Romain, Lam, Heather, Lamberti-Pasculli, Maria, Lang, Brian, Larner, James, Larrabee, Zachary, Leach, J. K., LeBlang, Suzanne, Leclercq, Delphine, Lee, Hak J., Lee, Jong-Hwan, Lehericy, Stéphane, Leighton, Wan, Leung, Steven, Lewis, Bobbi, Lewis, Matthew, Li, Dawei, Linn, Sabine, Lipsman, Nir, Liu, Hao-Li, Liu, Jingfei, Lopes, M. B., Lotz, Jeff, Lu, Xin, Lundt, Jonathan, Luo, Xi, Lustgarten, Lior, Lustig, Micheal, Macoskey, Jonathan, Madore, Bruno, Maev, Roman, Magat, Julie, Maimbourg, Guillaume, Maimon, Noam, Mainprize, Todd, Malayer, Jerry, Maples, Danny, Marquet, Fabrice, Marrocchio, Cristina, Marx, Mike, Mastorakos, Panagiotis, Mauri, Giovanni, McLean, Hailey, McMichael, John, Mead, Brian P., Melodelima, David, Melot-Dusseau, Sandrine, Menciassi, Arianna, Merrill, Robb, Meyer, Joshua, Midiri, Massimo, Miga, Michael, Migliore, Ilaria G., Miller, Eric, Minalga, Emilee, Moon, Hyungwon, Moore, David, Mourad, Pierre, Mouratidis, Petros, Mueller, Michael, Mugler, John, Muller, Sébastien, Namba, Hirofumi, Naor, Omer, Nassar, Maria, Nazai, Navid, Negron, Karina, Negussie, Ayele, Nguyen, Thai-Son, Nicolay, Klaas, Nikolaeva, Anastasia V., Oetgen, Matthew, Olive, Kenneth, Olumolade, Oluyemi, Orsi, Franco, Owens, Gabe, Ozilgen, Arda, Padegimas, Linas, Palermo, Carmine, Pan, Chia-Hsin, Pandey, Aditya, Papadakis, Georgios, Park, Chang K., Park, Sang M., Parker, Jonathon, Parvizi, Mohammad H., Pascal-Tenorio, Aurea, Patel, Janish, Patz, Sam, Payen, Thomas, Perich, Eloi, Pernot, Mathieu, Perol, David, Perry, James, Pillarisetty, Venu, Pioche, Mathieu, Pizzuto, Matthew, Plaksin, Michael, Plata, Juan, Price, Karl, Prince, Jessica, Przedborski, Serge, Quinones-Hinojosa, Alfredo, Ramachandran, Akhilesh, Ranjan, Ashish, Ravikumar, Vinod, Reichenbach, Juergen, Repasky, Elizabeth, Rezai, Ali, Ritter, Philippe, Rivoire, Michel, Rochman, Carrie, Rosenberg, Jarrett, Rosnitskiy, Pavel B., Ruiz, Antonio, Sahgal, Arjun, Samiotaki, Gesthimani, Sanghvi, Narendra, Santin, Mathieu D., Santos, Domiciano, Sasaki, Noboru, Sastra, Steve, Schade, George, Schall, Jeffrey, Schlesinger, Ilana, Schmitt, Paul, Schwaab, Julia, Scionti, Stephen, Scipione, Roberto, Scoarughi, Gian L., Scott, Serena, Sebeke, Lukas, Seifabadi, Reza, Seo, Jai, Sesenoglu-Laird, Ozge, Shah, Binit, Shahriari, Kian, Shaikh, Sumbul, Shea, Jill, Shi, Jiaqi, Shim, Jenny, Shinkov, Alexander, Shuman, Jillian, Silvestrini, Matthew, Sim, Changbeom, Sin, Vivian, Sinai, Alon, Singh, Manoj, Sinilshchikov, Ilya, Skalina, Karin, Slingluff, Craig, So, Po-Wah, Solomon, Stephen, Son, Keon H., Sperling, Scott, Stein, Ruben, Stein, Sherman, Stevens, Aaron, Stimec, Jennifer, Storm, Gert, Straube, William, Suelmann, Britt, Sutton, Jonathan, Svedin, Bryant, Takemasa, Ryuichi, Takiguchi, Mitsuyoshi, Tam, Emily, Tan, Jeremy, Tang, Xinyan, Tanter, Mickael, Tebebi, Pamela, Tehrani, Seruz, Temple, Michael, Teofilovic, Dejan, ter Haar, Gail, Terzi, Marina E., Thueroff, Stefan, Timbie, Kelsie, Tognarelli, Selene, Tretbar, Steffen, Trudeau, Maureen, Tsai, Yi-Chieh, Tsysar, Sergey A., Tucci, Samantha, Tuveson, David, Ushida, Takahiro, Vaessen, Paul, Vaillant, Fanny, Van Arsdell, Glen, van Breugel, Johanna, Van der Jeugd, Anneke, Van der Wall, Elsken, van Diest, Paul, van Stralen, Marijn, Varano, Gianluca, Velat, Manuela, Vidal-Jove, Joan, Vigna, Paolo D., Vignot, Alexandre, Vincenot, Jeremy, Vykhodtseva, Natalia, Wang, Bin, Wang, Han, Wang, Kevin, Wang, Qi, Wang, Qingguo, Wang, Shengping, Wang, Yak-Nam, Wang, Zhaorui, Wardlow, Rachel, Warren, Amy, Waszczak, Barbara, Watson, Katherine, Webb, Taylor, Wei-Bin, Shen, Wei, Kuo-Chen, Weiss, Steffen, Weissler, Yoni, Werner, Beat, Wesseling, Pieter, Williams, Noelle, Wilson, Emmanuel, Wintermark, Max, Witkamp, Arjen, Wong, Carlos, Wu, Jing-Fu, Wydra, Adrian, Xu, Alexis, Xu, Doudou, Xu, Su, Yang, Georgiana, Yang, Nai-Yi, Yao, Chen, Yarowsky, Paul, Ye, Patrick P., Yuldashev, Petr, Zaaroor, Menashe, Zachiu, Cornel, Zahos, Peter, Zangos, Stephan, Zhang, Dandan, Zhang, Hua, Zhang, Jimin, Zhang, Junhai, Zhang, Xi, Zhao, Li, Zhong, Pei, Zhuo, Jiachen, Zidowitz, Stephan, Zinke, Wolf, Zorgani, Ali, and Aerospace and Ocean Engineering

Subjects
GeneralLiterature_REFERENCE(e.g.,dictionaries,encyclopedias,glossaries)
Abstract

Published version

Published
2016

43. Identifying molecular phenotype of nucleus pulposus cells in human intervertebral disc with aging and degeneration

Author

Tang, Xinyan, Jing, Liufang, Richardson, William J, Isaacs, Robert E, Fitch, Robert D, Brown, Christopher R, Erickson, Melissa M, Setton, Lori A, and Chen, Jun

Subjects
Adult, Male, Aging, Nucleus Pulposus, Adolescent, Intervertebral Disc Degeneration, Middle Aged, Article, Phenotype, Antigens, CD, Humans, Female, Child, Collagen Type II, Biomarkers, Aged
Abstract

Previous study claimed that disc degeneration may be preceded by structure and matrix changes in the intervertebral disc (IVD) which coincide with the loss of distinct notochordally derived nucleus pulposus (NP) cells. However, the fate of notochordal cells and their molecular phenotype change during aging and degeneration in human are still unknown. In this study, a set of novel molecular phenotype markers of notochordal NP cells during aging and degeneration in human IVD tissue were revealed with immunostaining and flow cytometry. Furthermore, the potential of phenotype juvenilization and matrix regeneration of IVD cells in a laminin-rich pseudo-3D culture system were evaluated at day 28 by immunostaining, Safranin O, and type II collagen staining. Immunostaining and flow cytometry demonstrated that transcriptional factor Brachyury T, neuronal-related proteins (brain abundant membrane attached signal protein 1, Basp1; Neurochondrin, Ncdn; Neuropilin, Nrp-1), CD24, and CD221 were expressed only in juvenile human NP tissue, which suggested that these proteins may be served as the notochordal NP cell markers. However, the increased expression of CD54 and CD166 with aging indicated that they might be referenced as the potential biomarker for disc degeneration. In addition, 3D culture maintained most of markers in juvenile NP, and rescued the expression of Basp1, Ncdn, and Nrp 1 that disappeared in adult NP native tissue. These findings provided new insight into molecular profile that may be used to characterize the existence of a unique notochordal NP cells during aging and degeneration in human IVD cells, which will facilitate cell-based therapy for IVD regeneration. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:1316-1326, 2016.

Published
2016

46. 5th International Symposium on Focused Ultrasound

Author

Zaaroor, Menashe, primary, Sinai, Alon, additional, Goldsher, Dorit, additional, Eran, Ayelet, additional, Nassar, Maria, additional, Schlesinger, Ilana, additional, Parker, Jonathon, additional, Ravikumar, Vinod, additional, Ghanouni, Pejman, additional, Stein, Sherman, additional, Halpern, Casey, additional, Krishna, Vibhor, additional, Hargrove, Amelia, additional, Agrawal, Punit, additional, Changizi, Barbara, additional, Bourekas, Eric, additional, Knopp, Michael, additional, Rezai, Ali, additional, Mead, Brian, additional, Kim, Namho, additional, Mastorakos, Panagiotis, additional, Suk, Jung Soo, additional, Miller, Wilson, additional, Klibanov, Alexander, additional, Hanes, Justin, additional, Price, Richard, additional, Wang, Shutao, additional, Olumolade, Oluyemi, additional, Kugelman, Tara, additional, Jackson-Lewis, Vernice, additional, Karakatsani, Maria Eleni, additional, Han, Yang, additional, Przedborski, Serge, additional, Konofagou, Elisa, additional, Hynynen, Kullervo, additional, Aubert, Isabelle, additional, Leinenga, Gerhard, additional, Nisbet, Rebecca, additional, Hatch, Robert, additional, Van der Jeugd, Anneke, additional, Evans, Harrison, additional, Götz, Jürgen, additional, Van der Jeugd, Ann, additional, Fishman, Paul, additional, Yarowsky, Paul, additional, Frenkel, Victor, additional, Wei-Bin, Shen, additional, Nguyen, Ben, additional, Sanchez, Carlos Sierra, additional, Acosta, Camilo, additional, Chen, Cherry, additional, Wu, Shih-Ying, additional, Aryal, Muna, additional, Papademetriou, Iason T., additional, Zhang, Yong-Zhi, additional, Power, Chanikarn, additional, McDannold, Nathan, additional, Porter, Tyrone, additional, Kovacs, Zsofia, additional, Kim, Saejeong, additional, Jikaria, Neekita, additional, Qureshi, Farhan, additional, Bresler, Michele, additional, Frank, Joseph, additional, Odéen, Henrik, additional, Chiou, George, additional, Snell, John, additional, Todd, Nick, additional, Madore, Bruno, additional, Parker, Dennis, additional, Pauly, Kim Butts, additional, Marx, Mike, additional, Jonathan, Sumeeth, additional, Grissom, William, additional, Arvanitis, Costas, additional, Clement, Gregory, additional, de Bever, Joshua, additional, Payne, Allison, additional, Christensen, Douglas, additional, Maimbourg, Guillaume, additional, Santin, Mathieu David, additional, Houdouin, Alexandre, additional, Lehericy, Stéphane, additional, Tanter, Mickael, additional, Aubry, Jean Francois, additional, Federau, Christian, additional, Werner, Beat, additional, Paeng, Dong-Guk, additional, Xu, Zhiyuan, additional, Quigg, Anders, additional, Eames, Matt, additional, Jin, Changzhu, additional, Everstine, Ashli, additional, Sheehan, Jason, additional, Lopes, M. Beatriz, additional, Kassell, Neal, additional, Drake, James, additional, Price, Karl, additional, Lustgarten, Lior, additional, Sin, Vivian, additional, Mougenot, Charles, additional, Donner, Elizabeth, additional, Tam, Emily, additional, Hodaie, Mojgan, additional, Waspe, Adam, additional, Looi, Thomas, additional, Pichardo, Samuel, additional, Lee, Wonhye, additional, Chung, Yong An, additional, Jung, Yujin, additional, Song, In-Uk, additional, Yoo, Seung-Schik, additional, Kim, Hyun-Chul, additional, Lee, Jong-Hwan, additional, Caskey, Charles, additional, Zinke, Wolf, additional, Cosman, Josh, additional, Shuman, Jillian, additional, Schall, Jeffrey, additional, Aurup, Christian, additional, Chen, Hong, additional, Kamimura, Hermes, additional, Carneiro, Antonio, additional, Sun, Tao, additional, Nazai, Navid, additional, Patz, Sam, additional, Livingstone, Margaret, additional, Mainprize, Todd, additional, Huang, Yuexi, additional, Alkins, Ryan, additional, Chapman, Martin, additional, Perry, James, additional, Lipsman, Nir, additional, Bethune, Allison, additional, Sahgal, Arjun, additional, Trudeau, Maureen, additional, Liu, Hao-Li, additional, Hsu, Po-Hung, additional, Wei, Kuo-Chen, additional, Sutton, Jonathan, additional, Alexander, Phillip, additional, Miller, Eric, additional, Kobus, Thiele, additional, Carpentier, Alexandre, additional, Canney, Michael, additional, Vignot, Alexandre, additional, Beccaria, Kevin, additional, Leclercq, Delphine, additional, Lafon, Cyril, additional, Chapelon, Jean Yves, additional, Hoang-Xuan, Khe, additional, Delattre, Jean-Yves, additional, Idbaih, Ahmed, additional, Moore, David, additional, Xu, Alexis, additional, Schmitt, Paul, additional, Foley, Jessica, additional, Sukovich, Jonathan, additional, Cain, Charles, additional, Pandey, Aditya, additional, Chaudhary, Neeraj, additional, Camelo-Piragua, Sandra, additional, Allen, Steven, additional, Cannata, Jon, additional, Teofilovic, Dejan, additional, Bertolina, Jim, additional, Hall, Timothy, additional, Xu, Zhen, additional, Grondin, Julien, additional, Ferrera, Vincent, additional, ter Haar, Gail, additional, Mouratidis, Petros, additional, Repasky, Elizabeth, additional, Timbie, Kelsie, additional, Badr, Lena, additional, Campbell, Benjamin, additional, McMichael, John, additional, Buckner, Andrew, additional, Prince, Jessica, additional, Stevens, Aaron, additional, Bullock, Timothy, additional, Skalina, Karin, additional, Guha, Chandan, additional, Orsi, Franco, additional, Bonomo, Guido, additional, Vigna, Paolo Della, additional, Mauri, Giovanni, additional, Varano, Gianluca, additional, Schade, George, additional, Wang, Yak-Nam, additional, Pillarisetty, Venu, additional, Hwang, Joo Ha, additional, Khokhlova, Vera, additional, Bailey, Michael, additional, Khokhlova, Tatiana, additional, Sinilshchikov, Ilya, additional, Yuldashev, Petr, additional, Andriyakhina, Yulia, additional, Kreider, Wayne, additional, Maxwell, Adam, additional, Sapozhnikov, Oleg, additional, Partanen, Ari, additional, Lundt, Jonathan, additional, Preusser, Tobias, additional, Haase, Sabrina, additional, Bezzi, Mario, additional, Jenne, Jürgen, additional, Langø, Thomas, additional, Midiri, Massimo, additional, Mueller, Michael, additional, Sat, Giora, additional, Tanner, Christine, additional, Zangos, Stephan, additional, Guenther, Matthias, additional, Melzer, Andreas, additional, Menciassi, Arianna, additional, Tognarelli, Selene, additional, Cafarelli, Andrea, additional, Diodato, Alessandro, additional, Ciuti, Gastone, additional, Rothluebbers, Sven, additional, Schwaab, Julia, additional, Strehlow, Jan, additional, Mihcin, Senay, additional, Tretbar, Steffen, additional, Payen, Thomas, additional, Palermo, Carmine, additional, Sastra, Steve, additional, Olive, Kenneth, additional, Adams, Matthew, additional, Salgaonkar, Vasant, additional, Scott, Serena, additional, Sommer, Graham, additional, Diederich, Chris, additional, Vidal-Jove, Joan, additional, Perich, Eloi, additional, Ruiz, Antonio, additional, Velat, Manuela, additional, Melodelima, David, additional, Dupre, Aurelien, additional, Vincenot, Jeremy, additional, Yao, Chen, additional, Perol, David, additional, Rivoire, Michel, additional, Tucci, Samantha, additional, Mahakian, Lisa, additional, Fite, Brett, additional, Ingham, Elizabeth, additional, Tam, Sarah, additional, Hwang, Chang-il, additional, Tuveson, David, additional, Ferrara, Katherine, additional, Scionti, Stephen, additional, Chen, Lili, additional, Cvetkovic, Dusica, additional, Chen, Xiaoming, additional, Gupta, Roohi, additional, Wang, Bin, additional, Ma, Charlie, additional, Bader, Kenneth, additional, Haworth, Kevin, additional, Holland, Christy, additional, Sanghvi, Narendra, additional, Carlson, Roy, additional, Chen, Wohsing, additional, Chaussy, Christian, additional, Thueroff, Stefan, additional, Cesana, Claudio, additional, Bellorofonte, Carlo, additional, Wang, Qingguo, additional, Wang, Han, additional, Wang, Shengping, additional, Zhang, Junhai, additional, Bazzocchi, Alberto, additional, Napoli, Alessandro, additional, Staruch, Robert, additional, Bing, Chenchen, additional, Shaikh, Sumbul, additional, Nofiele, Joris, additional, Szczepanski, Debra, additional, Staruch, Michelle Wodzak, additional, Williams, Noelle, additional, Laetsch, Theodore, additional, Chopra, Rajiv, additional, Rosenberg, Jarrett, additional, Bitton, Rachelle, additional, LeBlang, Suzanne, additional, Meyer, Joshua, additional, Hurwitz, Mark, additional, Yarmolenko, Pavel, additional, Celik, Haydar, additional, Eranki, Avinash, additional, Beskin, Viktoriya, additional, Santos, Domiciano, additional, Patel, Janish, additional, Oetgen, Matthew, additional, Kim, AeRang, additional, Kim, Peter, additional, Sharma, Karun, additional, Chisholm, Alexander, additional, Aleman, Dionne, additional, Scipione, Roberto, additional, Temple, Michael, additional, Amaral, Joao Guilherme, additional, Endre, Ruby, additional, Lamberti-Pasculli, Maria, additional, de Ruiter, Joost, additional, Campbell, Fiona, additional, Stimec, Jennifer, additional, Gupta, Samit, additional, Singh, Manoj, additional, Hopyan, Sevan, additional, Czarnota, Gregory, additional, Brenin, David, additional, Rochman, Carrie, additional, Kovatcheva, Roussanka, additional, Vlahov, Jordan, additional, Zaletel, Katja, additional, Stoinov, Julian, additional, Bucknor, Matthew, additional, Rieke, Viola, additional, Shim, Jenny, additional, Koral, Korgun, additional, Lang, Brian, additional, Wong, Carlos, additional, Lam, Heather, additional, Shinkov, Alexander, additional, Hu, Jim, additional, Zhang, Xi, additional, Macoskey, Jonathan, additional, Ives, Kimberly, additional, Owens, Gabe, additional, Gurm, Hitinder, additional, Shi, Jiaqi, additional, Pizzuto, Matthew, additional, Dillon, Christopher, additional, Christofferson, Ivy, additional, Hilas, Elaine, additional, Shea, Jill, additional, Greillier, Paul, additional, Ankou, Bénédicte, additional, Bessière, Francis, additional, Zorgani, Ali, additional, Pioche, Mathieu, additional, Kwiecinski, Wojciech, additional, Magat, Julie, additional, Melot-Dusseau, Sandrine, additional, Lacoste, Romain, additional, Quesson, Bruno, additional, Pernot, Mathieu, additional, Catheline, Stefan, additional, Chevalier, Philippe, additional, Marquet, Fabrice, additional, Bour, Pierre, additional, Vaillant, Fanny, additional, Amraoui, Sana, additional, Dubois, Rémi, additional, Ritter, Philippe, additional, Haïssaguerre, Michel, additional, Hocini, Mélèze, additional, Bernus, Olivier, additional, Tebebi, Pamela, additional, Burks, Scott, additional, Milo, Blerta, additional, Gertner, Michael, additional, Zhang, Jimin, additional, Wong, Andrew, additional, Liu, Yu, additional, Kheirolomoom, Azadeh, additional, Seo, Jai, additional, Watson, Katherine, additional, Zhang, Hua, additional, Foiret, Josquin, additional, Borowsky, Alexander, additional, Xu, Doudou, additional, Thanou, Maya, additional, Centelles, Miguell, additional, Wright, Mike, additional, Amrahli, Maral, additional, So, Po-Wah, additional, Gedroyc, Wladyslaw, additional, Kneepkens, Esther, additional, Heijman, Edwin, additional, Keupp, Jochen, additional, Weiss, Steffen, additional, Nicolay, Klaas, additional, Grüll, Holger, additional, Nagle, Matthew, additional, Nikolaeva, Anastasia V., additional, Terzi, Marina E., additional, Tsysar, Sergey A., additional, Cunitz, Bryan, additional, Mourad, Pierre, additional, Downs, Matthew, additional, Yang, Georgiana, additional, Wang, Qi, additional, Chen, Johnny, additional, Farry, Justin, additional, Dixon, Adam, additional, Du, Zhongmin, additional, Dhanaliwala, Ali, additional, Hossack, John, additional, Ranjan, Ashish, additional, Maples, Danny, additional, Wardlow, Rachel, additional, Malayer, Jerry, additional, Ramachandran, Akhilesh, additional, Namba, Hirofumi, additional, Kawasaki, Motohiro, additional, Izumi, Masashi, additional, Kiyasu, Katsuhito, additional, Takemasa, Ryuichi, additional, Ikeuchi, Masahiko, additional, Ushida, Takahiro, additional, Crake, Calum, additional, Kothapalli, Satya V. V. N., additional, Leighton, Wan, additional, Wang, Zhaorui, additional, Gach, H. Michael, additional, Straube, William, additional, Altman, Michael, additional, Kim, Young-sun, additional, Lim, Hyo Keun, additional, Rhim, Hyunchul, additional, van Breugel, Johanna, additional, Braat, Manon, additional, Moonen, Chrit, additional, van den Bosch, Maurice, additional, Ries, Mario, additional, Marrocchio, Cristina, additional, Dababou, Susan, additional, Lee, Jae Young, additional, Chung, Hyun Hoon, additional, Kang, Soo Yeon, additional, Kang, Kook Jin, additional, Son, Keon Ho, additional, Zhang, Dandan, additional, Plata, Juan, additional, Jones, Peter, additional, Pascal-Tenorio, Aurea, additional, Bouley, Donna, additional, Bond, Aaron, additional, Dallapiazza, Robert, additional, Huss, Diane, additional, Warren, Amy, additional, Sperling, Scott, additional, Gwinn, Ryder, additional, Shah, Binit, additional, Elias, W. Jeff, additional, Curley, Colleen, additional, Zhang, Ying, additional, Negron, Karina, additional, Abounader, Roger, additional, Samiotaki, Gesthimani, additional, Tu, Tsang-Wei, additional, Papadakis, Georgios, additional, Hammoud, Dima, additional, Silvestrini, Matthew, additional, Wolfram, Frank, additional, Güllmar, Daniel, additional, Reichenbach, Juergen, additional, Hofmann, Denis, additional, Böttcher, Joachim, additional, Schubert, Harald, additional, Lesser, Thomas G., additional, Almquist, Scott, additional, Camarena, Francisco, additional, Jiménez-Gambín, Sergio, additional, Jiménez, Noé, additional, Chang, Jin Woo, additional, Chaplin, Vandiver, additional, Griesenauer, Rebekah, additional, Miga, Michael, additional, Ellens, Nicholas, additional, Airan, Raag, additional, Quinones-Hinojosa, Alfredo, additional, Farahani, Keyvan, additional, Feng, Xue, additional, Fielden, Samuel, additional, Zhao, Li, additional, Wintermark, Max, additional, Meyer, Craig, additional, Guo, Sijia, additional, Lu, Xin, additional, Zhuo, Jiachen, additional, Xu, Su, additional, Gullapalli, Rao, additional, Gandhi, Dheeraj, additional, Brokman, Omer, additional, Baek, Hongchae, additional, Kim, Hyungmin, additional, Leung, Steven, additional, Webb, Taylor, additional, Vykhodtseva, Natalia, additional, Nguyen, Thai-Son, additional, Park, Chang Kyu, additional, Park, Sang Man, additional, Jung, Na Young, additional, Kim, Min Soo, additional, Chang, Won Seok, additional, Jung, Hyun Ho, additional, Plaksin, Michael, additional, Weissler, Yoni, additional, Shoham, Shy, additional, Kimmel, Eitan, additional, Rosnitskiy, Pavel B., additional, Krupa, Steve, additional, Hazan, Eilon, additional, Naor, Omer, additional, Levy, Yoav, additional, Maimon, Noam, additional, Brosh, Inbar, additional, Kahn, Itamar, additional, Cahill, Jessica, additional, Colas, Elodie Constanciel, additional, Wydra, Adrian, additional, Maev, Roman, additional, Aly, Amirah, additional, Sesenoglu-Laird, Ozge, additional, Padegimas, Linas, additional, Cooper, Mark, additional, Waszczak, Barbara, additional, Tehrani, Seruz, additional, Slingluff, Craig, additional, Larner, James, additional, Andarawewa, Kumari, additional, Ozhinsky, Eugene, additional, Shah, Rutwik, additional, Krug, Roland, additional, Deckers, Roel, additional, Linn, Sabine, additional, Suelmann, Britt, additional, Witkamp, Arjen, additional, Vaessen, Paul, additional, van Diest, Paul, additional, Bartels, Lambertus W., additional, Bos, Clemens, additional, Borys, Nicolas, additional, Storm, Gert, additional, Van der Wall, Elsken, additional, Farr, Navid, additional, Alnazeer, Moez, additional, Katti, Prateek, additional, Wood, Bradford, additional, Farrer, Alexis, additional, Ferrer, Cyril, additional, de Senneville, Baudouin Denis, additional, van Stralen, Marijn, additional, Liu, Jingfei, additional, Leach, J. Kent, additional, Zidowitz, Stephan, additional, Lee, Hsin-Lun, additional, Hsu, Fang-Chi, additional, Kuo, Chia-Chun, additional, Jeng, Shiu-Chen, additional, Chen, Tung-Ho, additional, Yang, Nai-Yi, additional, Chiou, Jeng-Fong, additional, Kao, Yi-tzu, additional, Pan, Chia-Hsin, additional, Wu, Jing-Fu, additional, Tsai, Yi-Chieh, additional, Johnson, Sara, additional, Li, Dawei, additional, He, Ye, additional, Karakitsios, Ioannis, additional, Schwenke, Michael, additional, Demedts, Daniel, additional, Xiao, Xu, additional, Cavin, Ian, additional, Minalga, Emilee, additional, Merrill, Robb, additional, Hadley, Rock, additional, Ramaekers, Pascal, additional, de Greef, Martijn, additional, Shahriari, Kian, additional, Parvizi, Mohammad Hossein, additional, Asadnia, Kiana, additional, Chamanara, Marzieh, additional, Kamrava, Seyed Kamran, additional, Chabok, Hamid Reza, additional, Stein, Ruben, additional, Muller, Sébastien, additional, Tan, Jeremy, additional, Zachiu, Cornel, additional, Erasmus, Hans-Peter, additional, Van Arsdell, Glen, additional, Benson, Lee, additional, Jang, Kee W., additional, Angstadt, Mary, additional, Lewis, Bobbi, additional, McLean, Hailey, additional, Hoogenboom, Martijn, additional, Eikelenboom, Dylan, additional, den Brok, Martijn, additional, Wesseling, Pieter, additional, Heerschap, Arend, additional, Fütterer, Jurgen, additional, Adema, Gosse, additional, Wang, Kevin, additional, Zhong, Pei, additional, Joy, Joyce, additional, McLeod, Helen, additional, Kim, Harry, additional, Lewis, Matthew, additional, Ozilgen, Arda, additional, Zahos, Peter, additional, Coughlin, Dezba, additional, Tang, Xinyan, additional, Lotz, Jeff, additional, Jedruszczuk, Kathleen, additional, Gulati, Amitabh, additional, Solomon, Stephen, additional, Kaye, Elena, additional, Mugler, John, additional, Barbato, Gaetano, additional, Scoarughi, Gian Luca, additional, Corso, Cristiano, additional, Gorgone, Alessandro, additional, Migliore, Ilaria Giuseppina, additional, Larrabee, Zachary, additional, Hananel, Arik, additional, Aubry, Jean-Francois, additional, Negussie, Ayele, additional, Wilson, Emmanuel, additional, Seifabadi, Reza, additional, Moon, Hyungwon, additional, Kang, Jeeun, additional, Sim, Changbeom, additional, Chang, Jin Ho, additional, Kim, Hyuncheol, additional, Lee, Hak Jong, additional, Sasaki, Noboru, additional, Takiguchi, Mitsuyoshi, additional, Sebeke, Lukas, additional, Luo, Xi, additional, de Jager, Bram, additional, Heemels, Maurice, additional, Abraham, Christopher, additional, Curiel, Laura, additional, Berriet, Rémi, additional, Janát-Amsbury, Margit, additional, Corea, Joseph, additional, Ye, Patrick Peiyong, additional, Arias, Ana Clauda, additional, Lustig, Micheal, additional, and Svedin, Bryant, additional

Published
2016
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50. Analysis of the shaping law of numerical controlled electrochemical contour evolution machining

Author

Tang Xinyan, Xu Jiawen, and Kang Min

Subjects
Machining process, Engineering, Machining, business.industry, Differential equation, law, Coincident, Development (differential geometry), Electrochemical machining, business, Cathode, law.invention
Abstract

Numerical Controlled Electrochemical Contour Evolution Machining (NC-ECCEM) is one of the most important development in Electrochemical Machining (ECM), the study on its shaping law is significant for improving the machining accuracy of workpiece profile. In this paper, the shaping law of machining grooves by use of a kind of inner-spraying cathode with triangle section was studied. First, the basic differential equations of shaping law in the case of cathode movement were established. Then, considering the structure of the cathode, the methods for calculating the bottom gap and the side gap in machining vertical grooves, inclined grooves and circular grooves were given respectively. Finally, the experiments of machining vertical grooves, inclined grooves and circular grooves were carried out. Experiments show that the calculated bottom gaps are better consistent with the actual values in machining process, and the calculated side gaps are bigger than actual values, but the change trend of calculated side gaps with machining process parameters is coincident with the actual side gap change trend. In order to estimate correctly the side gap, a method for modifying calculated side gaps was put forward. Through the experiments, a special shaping characteristic that the side gap is less than bottom gap in NC-ECCEM was found too.

Published
2006

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