1. Nuclear factor I-A represses expression of the cell adhesion molecule L1
- Author
-
Tanja Schneegans, Richard M. Gronostajski, Melitta Schachner, Thomas Tilling, Moritz Hentschke, and Uwe Borgmeyer
- Subjects
Gene isoform ,lcsh:QH426-470 ,Molecular Sequence Data ,Down-Regulation ,Neural Cell Adhesion Molecule L1 ,Biology ,Cell Line ,Mice ,Neuroblastoma ,Cricetinae ,Gene expression ,Research article ,Animals ,Protein Isoforms ,Electrophoretic mobility shift assay ,Amino Acid Sequence ,lcsh:QH573-671 ,Transcription factor ,Molecular Biology ,Reporter gene ,Binding Sites ,Nuclear factor I ,Base Sequence ,lcsh:Cytology ,Brain ,Molecular biology ,Introns ,Cell biology ,lcsh:Genetics ,NFI Transcription Factors ,Organ Specificity ,Neural cell adhesion molecule ,Chromatin immunoprecipitation - Abstract
Background The neural cell adhesion molecule L1 plays a crucial role in development and plasticity of the nervous system. Neural cells thus require precise control of L1 expression. Results We identified a full binding site for nuclear factor I (NFI) transcription factors in the regulatory region of the mouse L1 gene. Electrophoretic mobility shift assay (EMSA) showed binding of nuclear factor I-A (NFI-A) to this site. Moreover, for a brain-specific isoform of NFI-A (NFI-A bs), we confirmed the interaction in vivo using chromatin immunoprecipitation (ChIP). Reporter gene assays showed that in neuroblastoma cells, overexpression of NFI-A bs repressed L1 expression threefold. Conclusion Our findings suggest that NFI-A, in particular its brain-specific isoform, represses L1 gene expression, and might act as a second silencer of L1 in addition to the neural restrictive silencer factor (NRSF).
- Published
- 2009