4 results on '"Tao-xing Shi"'
Search Results
2. Decision support system for the response to infectious disease emergencies based on WebGIS and mobile services in China.
- Author
-
Ya-pin Li, Li-qun Fang, Su-qing Gao, Zhen Wang, Hong-wei Gao, Peng Liu, Ze-Rui Wang, Yan-Li Li, Xu-Guang Zhu, Xin-Lou Li, Bo Xu, Yin-Jun Li, Hong Yang, Sake J de Vlas, Tao-Xing Shi, and Wu-Chun Cao
- Subjects
Medicine ,Science - Abstract
BackgroundFor years, emerging infectious diseases have appeared worldwide and threatened the health of people. The emergence and spread of an infectious-disease outbreak are usually unforeseen, and have the features of suddenness and uncertainty. Timely understanding of basic information in the field, and the collection and analysis of epidemiological information, is helpful in making rapid decisions and responding to an infectious-disease emergency. Therefore, it is necessary to have an unobstructed channel and convenient tool for the collection and analysis of epidemiologic information in the field.Methodology/principal findingsBaseline information for each county in mainland China was collected and a database was established by geo-coding information on a digital map of county boundaries throughout the country. Google Maps was used to display geographic information and to conduct calculations related to maps, and the 3G wireless network was used to transmit information collected in the field to the server. This study established a decision support system for the response to infectious-disease emergencies based on WebGIS and mobile services (DSSRIDE). The DSSRIDE provides functions including data collection, communication and analyses in real time, epidemiological detection, the provision of customized epidemiological questionnaires and guides for handling infectious disease emergencies, and the querying of professional knowledge in the field. These functions of the DSSRIDE could be helpful for epidemiological investigations in the field and the handling of infectious-disease emergencies.Conclusions/significanceThe DSSRIDE provides a geographic information platform based on the Google Maps application programming interface to display information of infectious disease emergencies, and transfers information between workers in the field and decision makers through wireless transmission based on personal computers, mobile phones and personal digital assistants. After a 2-year practice and application in infectious disease emergencies, the DSSRIDE is becoming a useful platform and is a useful tool for investigations in the field carried out by response sections and individuals. The system is suitable for use in developing countries and low-income districts.
- Published
- 2013
- Full Text
- View/download PDF
3. [Different effects of long-term and short-term repeated restraints on the hematopoietic stem cells in mice]
- Author
-
Chen-Ke, Ma, Xian, Wu, Zhe, Xu, Jian, Liu, Ji-Yan, Zhang, and Tao-Xing, Shi
- Subjects
Mice, Inbred C57BL ,Restraint, Physical ,Mice ,CD11b Antigen ,Animals ,Antigens, Ly ,Bone Marrow Cells ,Hematopoietic Stem Cells ,Stress, Psychological ,Cell Proliferation - Abstract
Humans with chronic psychological stress are prone to develop multiple disorders of body function including impairment of immune system. Chronic psychological stress has been reported to have negative effects on body immune system. However, the underlying mechanisms have not been clearly demonstrated. All immune cells are derived from hematopoietic stem cells (HSC) in the bone marrow, including myeloid cells which comprise the innate immunity as a pivotal component. In this study, to explore the effects of chronic psychological stress on HSC and myeloid cells, different repeated restraint sessions were applied, including long-term mild restraint in which mice were individually subjected to a 2 h restraint session twice daily (morning and afternoon/between 9:00 and 17:00) for 4 weeks, and short-term vigorous restraint in which mice were individually subjected to a 16 h restraint session (from 17:00 to 9:00 next day) for 5 days. At the end of restraint, mice were sacrificed and the total cell numbers in the bone marrow and peripheral blood were measured by cell counting. The proportions and absolute numbers of HSC (Lin
- Published
- 2020
4. Different strategies for preparation of non-tagged rV270 protein and its efficacy against Yersinia pestis challenge
- Author
-
Yonghai Yang, Baizhong Cui, Wang Wang, Tao-Xing Shi, Hu Wang, Zhizhen Qi, Xiaoyi Wang, Ziwen Zhu, Ruifu Yang, Benchuan Wu, Qingwen Zhang, Zuyun Wang, Zhaobiao Guo, Yefeng Qiu, and Ruixia Dai
- Subjects
Enteropeptidase ,Pore Forming Cytotoxic Proteins ,Yersinia pestis ,Health, Toxicology and Mutagenesis ,Protein subunit ,Recombinant Fusion Proteins ,Blotting, Western ,Genetic Vectors ,Molecular Sequence Data ,Protein Engineering ,law.invention ,Fusion gene ,Mice ,Thrombin ,Affinity chromatography ,law ,medicine ,Escherichia coli ,Animals ,LcrV ,Amino Acid Sequence ,Cloning, Molecular ,Antigens, Bacterial ,Mice, Inbred BALB C ,Plague ,Plague Vaccine ,biology ,Chemistry ,Public Health, Environmental and Occupational Health ,biology.organism_classification ,Virology ,Molecular biology ,Antibodies, Bacterial ,Survival Analysis ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Vaccines, Subunit ,Recombinant DNA ,Electrophoresis, Polyacrylamide Gel ,Female ,medicine.drug ,Plasmids - Abstract
Objective LcrV is an important component for the development of a subunit vaccine against plague. To reduce immunosuppressive activity of LcrV, a recombinant LcrV variant lacking amino acids 271 to 326 (rV270) was prepared by different methods in this study. Methods A new strategy that produced non-tagged or authentic rV270 protein was designed by insertion of rV270-thrombin-hexahistidine fusion gene into the vector pET24a, or by insertion of hexahistidine-enterokinase-rV270 or hexahistitine-factor Xa-rV270 fusion gene into the vector pET32a. After Co2+ affinity chromatography, a purification strategy was developed by cleavage of His tag on column, following Sephacryl S-200HR column filtration chromatography. Results Removal of His tag by thrombin, enterokinase and factor Xa displayed a yield of 99.5%, 32.4% and 15.3%, respectively. Following Sephacryl S-200HR column filtration chromatography, above 97% purity of rV270 protein was obtained. Purified rV270 that was adsorbed to 25% (v/v) Al(OH)3 adjuvant in phosphate-buffered saline (PBS) induced very high titers of antibody to rV270 in BALB/c mice and protected them (100% survival) against subcutaneous challenge with 106 CFU of Y. pestis virulent strain 141. Conclusion The completely authentic rV270 protein can be prepared by using enterokinase or factor Xa, but they exhibited extremely low cleavage activity to the corresponding recognition site. Thrombin cleavage is an efficient strategy to prepare non-tagged rV270 protein and can be easily operated in a large scale due to its relatively low cost and high cleavage efficacy. The recombinant rV270 can be used as a key component to develop a subunit vaccine of plague.
- Published
- 2010
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.