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The genus Salmonellahas more than 2,600 serovars, and this trait is important when considering interventions for Salmonellacontrol. Bacteriophages that are used for biocontrol must have an exclusively lytic cycle and the ability to lyse several Salmonellaserovars under a wide range of environmental conditions. Salmonellaphages were isolated and characterized from 34 backyard production systems (BPSs) with a history of Salmonellainfections. BPSs were visited once, and cloacal or fecal samples were processed for phage isolation. Four hosts, Salmonellaserovars Enteritidis, Heidelberg, Infantis, and Typhimurium, were used for phage isolation. The host range of the phages was later characterized with a panel of 23 Salmonellaserovars (serovar diversity set) and 31 isolates obtained from the same farms (native set). Genetic relatedness for 10 phages with a wide host range was characterized by restriction fragment length polymorphism, and phages clustered based on the host range. We purified 63 phages, and 36 phage isolates were obtained on SalmonellaEnteritidis, 16 on SalmonellaHeidelberg, and 11 on SalmonellaInfantis. Phages were classified in three clusters: (i) phages with a wide host range (cluster I), (ii) phages that lysed the most susceptible Salmonellaserovars (serogroup D) and other isolates (cluster II), and (iii) phages that lysed only isolates of serogroup D (cluster III). The most susceptible Salmonellaserovars were Enteritidis, Javiana, and Dublin. Seven of 34 farms yielded phages with a wide host range, and these phages had low levels of genetic relatedness. Our study showed an adaptation of the phages in the sampled BPSs to serogroup D Salmonellaisolates and indicated that isolation of Salmonellaphages with wide host range differs by farm. A better understanding of the factors driving the Salmonellaphage host range could be useful when designing risk-based sampling strategies to obtain phages with a wide lytic host range for biocontrol purposes.