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High quality forages produced in the southeastern U.S. can benefit from being harvested as baleage when environmental conditions are not favorable for hay production. Current recommendations are to feed baleage within 9 mo of harvesting; however, wet summers or mild winters can reduce the need for utilizing stored forage in this timely manner. Limited data exist on the nutritional value of baleage stored beyond a 9-mo period. Therefore, the objective of this research is to determine if storage length affects the nutritive value of baleage in the southeastern U.S. This study was conducted from 2016–2020 at the University of Georgia Tifton Campus, using ‘Tifton-85’ bermudagrass baleage (BG) and ‘Bulldog 805’ alfalfa-bermudagrass mixed baleage (BGA) harvested on a 28–35-d interval, baled at approximately 55% moisture, and individually wrapped for storage. Bales were marked by treatment, sampled prior to wrapping for initial determination (INT), and sampled again post fermentation at 6-wk, 9-mo and 12-mo timepoints for nutritive value analyses. Additionally, a subset of bales harvested in June and August of 2017 and 2018 were maintained and sampled at 24-mo time point. Statistical analysis was conducted using the PROC MIXED procedure of SAS 9.4. Nutritive analysis showed a difference in the CP% between pre and post fermentation events for BGA (INT=22±2.39 and 6-wk, 9-mo, 12-mo=20±2.39; P < 0.01), while no difference was found for BG. TDN% was different only at 6 wk for BGA (66±0.63; P = 0.03) compared to all other timepoints, with no difference for BG. The 24mo storage analysis showed a decline in the CP% and TDN% for both BGA and BG from pre and post fermentation. Results from this study conclude there no effect on the nutritive value of baleage stored up to 12 mo post fermentation. Maintaining bale integrity can be attributed to the decline in nutritive value observed when stored longer than 12 mo.

Background Ebola virus disease (EVD) survivors are at risk for uveitis during convalescence. Vision loss has been observed following uveitis due to cataracts. Since Ebola virus (EBOV) may persist in the ocular fluid of EVD survivors for an unknown duration, there are questions about the safety and feasibility of vision restorative cataract surgery in EVD survivors. Methods We conducted a cross-sectional study of EVD survivors anticipating cataract surgery and patients with active uveitis to evaluate EBOV RNA persistence in ocular fluid, as well as vision outcomes post cataract surgery. Patients with aqueous humor that tested negative for EBOV RNA were eligible to proceed with manual small incision cataract surgery (MSICS). Findings We screened 137 EVD survivors from June 2016 – August 2017 for enrolment. We enrolled 50 EVD survivors; 46 with visually significant cataract, 1 with a subluxated lens, 2 with active uveitis and 1 with a blind painful eye due to uveitis. The median age was 24.0 years (IQR 17–35) and 35 patients (70%) were female. The median logMAR visual acuity (VA) was 3.0 (Snellen VA Hand motions; Interquartile Range, IQR: 1.2-3.0, Snellen VA 20/320 – Hand motions). All patients tested negative for EBOV RNA by RT-PCR in aqueous humor/vitreous fluid and conjunctiva at a median of 19 months (IQR 18-20) from EVD diagnosis in Phase 1 of ocular fluid sampling and 34 months (IQR 32-36) from EVD diagnosis in Phase 2 of ocular fluid sampling. Thirty-four patients underwent MSICS, with a preoperative median VA improvement from hand motions to 20/30 at three-month postoperative follow-up (P, Highlights • Ebola virus disease (EVD) survivors are at-risk for severe vision impairment due to uveitis and subsequent cataract development. • Fifty EVD survivors underwent ocular fluid sampling and tested negative for Ebola virus by RT-PCR. • Thirty-four survivors underwent cataract surgery with excellent safety measures and vision restorative outcomes. Ebola virus disease (EVD) survivors are at high risk for uveitis, an inflammatory condition affecting the eye that may lead to severe vision impairment, often from cataract. Because persistent Ebola virus (EBOV) has been identified within the immune privileged eye, understanding the prevalence of intraocular EBOV persistence is significant for patients, providers, and public health policy. In this study, 50 EVD survivors tested negative for EBOV by RT-PCR of their intraocular fluid. Thirty-four underwent cataract surgery with vision restorative outcomes. These findings improve our ability to impact vision care and quality-of-life for thousands of EVD survivors at-risk for eye disease.