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Your search keyword '"Teeuwen, R.L.M."' showing total 9 results
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9 results on '"Teeuwen, R.L.M."'

1. Tuning Ice Nucleation with Supercharged Polypeptides

Author

Yang, H.G., Ma, C., Li, K.Y., Liu, K., Loznik, M., Teeuwen, R.L.M., van Hest, J.C.M., Zhou, X., Herrmann, A., Wang, J.J., Yang, H.G., Ma, C., Li, K.Y., Liu, K., Loznik, M., Teeuwen, R.L.M., van Hest, J.C.M., Zhou, X., Herrmann, A., and Wang, J.J.

Abstract

Contains fulltext : 159886.pdf (publisher's version ) (Closed access)

Published
2016

2. Protein assembly in microreators mediated by Elastin-like polypeptides

Author

Teeuwen, R.L.M., Hest, J.C.M. van, Zuilhof, H., Wolf, F.A. de, and Radboud University Nijmegen

Subjects
Bio-Organic Chemistry
Abstract

Contains fulltext : 75961.pdf (Publisher’s version ) (Open Access) RU Radboud Universiteit Nijmegen, 11 november 2009 Promotores : Hest, J.C.M. van, Zuilhof, H. Co-promotor : Wolf, F.A. de 143 p.

Published
2009

3. 'Clickable' polymersomes

Author

Opsteen, J.A., Brinkhuis, R.P., Teeuwen, R.L.M., Lowik, D.W.P.M., and Hest, J.C.M. van

Subjects
Bio-Organic Chemistry
Abstract

Contains fulltext : 34590.pdf (Publisher’s version ) (Closed access)

Published
2007

4. Positional assembly of temperature-responsive biopolymers

Author

Teeuwen, R.L.M., Zuilhof, H., de Wolf, F.A., and van Hest, J.C.M.

Subjects
AFSG Biobased Products, Organic Chemistry, Life Science, Organische Chemie
Abstract

Incorporation of enzymic cascade reactions in a microchannel for org. synthesis can be achieved if spatially controlled immobilization of a series of enzymes is possible. This project attempts positional assembly of enzymes using the inverse phase transition temp. (Tt) of elastin-like proteins (ELPs). Increasing the temp. results in aggregation and assembly of ELPs, which can be very precisely controlled. Protein engineering is used as a method to produce ELPs and their fusion proteins. These fusion proteins can be assembled onto surfaces modified with the complementary ELP, when the surface is heated above Tt. Two elastin variants were constructed that each showed the expected inverse transition behavior. Fusions of these ELPs with fluorescent proteins generated proteins with inverse transition behavior that could be controlled using varying concns. of NaCl. We are currently investigating the possibilities to functionalize microchannels with ELPs to enable positional assembly of proteins.

Published
2007

5. Protein assembly in microreators mediated by Elastin-like polypeptides

Author

Hest, J.C.M. van, Zuilhof, H., Wolf, F.A. de, Teeuwen, R.L.M., Hest, J.C.M. van, Zuilhof, H., Wolf, F.A. de, and Teeuwen, R.L.M.

Abstract

RU Radboud Universiteit Nijmegen, 11 november 2009, Promotores : Hest, J.C.M. van, Zuilhof, H. Co-promotor : Wolf, F.A. de, Contains fulltext : 75961.pdf (publisher's version ) (Open Access)

Published
2009

6. Clickable elastins: elastin-like polypeptides functionalized with azide or alkyne groups

Author

Teeuwen, R.L.M., Berkel, S.S. van, Dulmen, T.H.H. van, Schoffelen, S., Meeuwissen, S.A., Zuilhof, H., Wolf, F.A. de, Hest, J.C.M. van, Teeuwen, R.L.M., Berkel, S.S. van, Dulmen, T.H.H. van, Schoffelen, S., Meeuwissen, S.A., Zuilhof, H., Wolf, F.A. de, and Hest, J.C.M. van

Abstract

Contains fulltext : 75310.pdf (publisher's version ) (Closed access)

Published
2009

9. Secreted production of an elastin-like polypeptide by Pichia pastoris

Author

Schipperus, R., Teeuwen, R.L.M., Werten, M.W.T., Eggink, G., de Wolf, F.A., Schipperus, R., Teeuwen, R.L.M., Werten, M.W.T., Eggink, G., and de Wolf, F.A.

Abstract

Elastin-like polypeptides (ELPs) are biocompatible designer polypeptides with inverse temperature transition behavior in solution. They have a wide variety of possible applications and a potential medical importance. Currently, production of ELPs is done at lab scale in Escherichia coli shake flask cultures. With a view to future large scale production, we demonstrate secreted production of ELPs in methanol-induced fed-batch cultures of Pichia pastoris and purification directly from the culture medium. The production of ELPs by P. pastoris proved to be pH dependent within the experimental pH range of pH 3 to 7, as an increasing yield was found in cultures grown at higher pH. Because ELP produced at pH 7 was partly degraded, a pH optimum for production of ELP was found at pH 6 with a yield of 255 mg of purified intact ELP per liter of cell-free medium

Published
2009

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