26 results on '"Teeuwsen, V."'
Search Results
2. Intracellular immunoglobulin G 'pseudocrystals' in a patient with chronic B-cell leukemia.
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Den Ottolander, Gerard J., Brederoo, Pieter, Schuurman, Ruud K. B., Teeuwsen, Vera J. P., Schuit, Henrka R. E., Van Der Meulen, Jannes, Jansen, Jan, Hijmans, Willy, den Ottolander, G J, Brederoo, P, Schuurman, R K, Teeuwsen, V J, Schuit, H R, van der Meulen, J, Jansen, J, and Hijmans, W
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- 1986
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3. Differential cytotoxic T-lymphocyte (CTL) responses in HIV-1 immunised sibling chimpanzees with shared MHC haplotypes
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Balla-Jhagjhoorsingh, S., Mooij, P., Koopman, G., Haaksma, T., Teeuwsen, V., Heeney, J., and Bontrop, R.
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- 1999
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4. Immune strategies utilized by lentivirus infected chimpanzees to resist progression to AIDS
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Heeney, J., Bogers, W., Buijs, L., Dubbes, R., Haaft, P. Ten, Koornstra, W., Niphuis, H., Nara, P., and Teeuwsen, V.
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- 1996
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5. The role of major histocompatibility complex polymorphisms on SIV infection in rhesus macaques
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Bontrop, R. E., Otting, N., Niphuis, H., Noort, R., Teeuwsen, V., and Heeney, J. L.
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- 1996
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6. De energiebehoefte van de mens III. Uitkomsten van de tweede proefserie en discussie
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Vogt, J.E., Teeuwsen, V., Deurenberg, P., van der Beek, E., and van Es, A.J.H.
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Human and Animal Physiology ,Humane Voeding & Gezondheid ,Fysiologie van Mens en Dier ,Life Science ,Human Nutrition & Health - Published
- 1982
7. Human anti-idiotypic T lymphocyte clones are activated by autologous anti- rabies virus antibodies presented in association with HLA-DQ molecules.
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UytdeHaag, F., Claassen, I.J.Th.M. (Ivo), Bunschoten, H., Loggen, H., Ottenhoff, T., Teeuwsen, V., Osterhaus, A.D.M.E. (Albert), UytdeHaag, F., Claassen, I.J.Th.M. (Ivo), Bunschoten, H., Loggen, H., Ottenhoff, T., Teeuwsen, V., and Osterhaus, A.D.M.E. (Albert)
- Abstract
The regulatory function of antigen-specific T cells in human antibody responses to protein and carbohydrate determinants of many viral and bacterial antigens has extensively been studied in systems involving in vitro triggering of B cells by antigens or polyclonal activators. Although amply documented in experimental murine models, the existence of T helper cells with receptor specificity for idiotypic determinants of B cell immunoglobulins has not been demonstrated in a human system. We are interested in T helper cell recognition of idiotypic determinants of virus-specific antibody, secreted by human B cells in response to viral antigens, and in the role which such idiotype-specific T helper cells play, alone or in concert with virus-specific T helper cells, to regulate the antibody response. Understanding of the function of different T helper cell subsets in an anti-viral antibody response and especially of the mechanisms of idiotype recognition by T cells is important for the development, and future application in man of idiotype vaccines, the potential of which has been indicated for different pathogens in several animal species. It was realized that for the efficient characterization of each of the T helper cell subsets, the availability of cloned populations of T cells would be inevitable. Furthermore, we argued that if, as predicted by Jerne, idiotype recognizing T helper cells are involved in physiological idiotype regulation in the course of an immune response--e.g., following encounter with viru
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- 1987
8. Human energy metabolism below, near and above energy equilibrium
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van Es, A.J.H., Vogt, J.E., Niessen, C.H., Veth, J., Rodenburg, L., Teeuwsen, V., Deurenberg, P., Hautvast, J.G.A.J., Dhuyvetter, J.H.M., van der Beek, H., van Es, A.J.H., Vogt, J.E., Niessen, C.H., Veth, J., Rodenburg, L., Teeuwsen, V., Deurenberg, P., Hautvast, J.G.A.J., Dhuyvetter, J.H.M., and van der Beek, H.
- Published
- 1984
9. Vaccine protection and reduced virus load from heterologous macaque-propagated SIV challenge
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Jonathan Heeney, Holterman L, ten Haaft P, Dubbes R, Koornstra W, Teeuwsen V, Bourquin P, Norley S, and Niphuis H
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Vaccines, Synthetic ,Time Factors ,Species Specificity ,Neutralization Tests ,SAIDS Vaccines ,Simian Acquired Immunodeficiency Syndrome ,Animals ,Immunization ,Simian Immunodeficiency Virus ,Vaccines, Attenuated ,Macaca mulatta - Abstract
The efficacy of vaccine protection afforded by live attenuated vaccines was tested by heterologous SIVtno8980 challenge following successful protection against homologous SIVmac32H challenge. Animals immunized with the attenuated SIVmacBK28 molecular clone were asymptomatic and virus isolation negative by quantitative virus isolation prior to challenge. Two groups of four animals previously immunized 5 years and 4 months (respectively) were challenged with 100 MID50 of SIVtno8980, as was a third group of four naive controls. All control animals that were challenged developed high levels of plasma antigenemia within 2 weeks of challenge and developed rapid Th/m cell loss whereas vaccinated animals did not. Quantitative virus isolation from peripheral blood mononuclear cells revealed that one of four animals in each group became virus isolation positive but that the virus load in the two vaccinated animals was markedly lower than in nonvaccinated controls. Studies are underway to determine the duration and immunological correlates of protection from AIDS.
10. A recombinant HIV-1 virus-like particle vaccine: from concepts to a field study
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Wagner R, Deml L, Teeuwsen V, Jonathan Heeney, Yiming S, and Wolf H
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AIDS Vaccines ,Acquired Immunodeficiency Syndrome ,China ,Mice ,Mice, Inbred BALB C ,Vaccines, Synthetic ,HIV-1 ,Animals ,Humans
11. Conserved CTL epitopes shared between HIV-infected human long-term survivors and chimpanzees
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Balla-Jhagjhoorsingh, S. S., Koopman, G., Mooij, P., Haaksma, T. G. M., Teeuwsen, V. J. P., Ronald Bontrop, and Heeney, J. L.
12. Analysis of the antigen- and mitogen-induced differentiation of B lymphocytes from asymptomatic human immunodeficiency virus-seropositive male homosexuals. Discrepancy between T cell-dependent and T cell-independent activation.
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Teeuwsen, V J, primary, Logtenberg, T, additional, Siebelink, K H, additional, Lange, J M, additional, Goudsmit, J, additional, Uytdehaag, F G, additional, and Osterhaus, A D, additional
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- 1987
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13. Conserved CTL epitopes shared between HIV-infected human long-term survivors and chimpanzees.
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Balla-Jhagjhoorsingh SS, Koopman G, Mooij P, Haaksma TG, Teeuwsen VJ, Bontrop RE, and Heeney JL
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- Acquired Immunodeficiency Syndrome genetics, Acquired Immunodeficiency Syndrome veterinary, Amino Acid Sequence, Animals, Cytotoxicity, Immunologic genetics, Epitope Mapping, Epitopes, T-Lymphocyte genetics, Epitopes, T-Lymphocyte isolation & purification, Gene Products, gag immunology, Histocompatibility Antigens Class I genetics, Histocompatibility Antigens Class I immunology, Humans, Immunophenotyping, Molecular Sequence Data, Primate Diseases genetics, Primate Diseases immunology, T-Lymphocytes, Cytotoxic chemistry, T-Lymphocytes, Cytotoxic metabolism, Acquired Immunodeficiency Syndrome immunology, Conserved Sequence immunology, Epitopes, T-Lymphocyte metabolism, HIV Long-Term Survivors, HIV-1 immunology, Pan troglodytes immunology, T-Lymphocytes, Cytotoxic immunology
- Abstract
Certain HIV-1 infected humans that do not progress to AIDS have been documented to share particular MHC class I alleles that appear to correlate with long-term survival. HIV-1-infected chimpanzees are relatively resistant to progression to AIDS. Out of a group of 10 chimpanzees with CTL activity and nonprogressive HIV-1 infection, 2 animals with prominent cytolytic CD3+CD8+ T cell responses to HIV-1 Ags were studied in detail. Characterization of these CTL revealed that they contained the granzymes A and B, T cell intracellular Ag-1, and perforin and induced calcium-dependent cytolysis that correlated with the presence of apoptotic nuclei in target cells. These CTL responses were directed against two gagpeptides, which were found to be identical to previously described epitopes recognized in the context of HLA-B27 and HLA-B57 molecules. The latter two restriction elements occur with increased frequency in human long-term survivor cohorts. Phylogenetic comparisons revealed that the chimpanzee restriction elements, Patr-B*02and -B*03, described here do not show any obvious similarity with the HLA-B*27 and -B*57 alleles, suggesting that CTL responses to HIV-1 in distinct primate species may be controlled by different types of HLA-B-like molecules. The CTL responses in these two chimpanzees are directed, however, against highly conserved epitopes mapping across the majority of HIV-1 clades.
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- 1999
14. beta-chemokines and neutralizing antibody titers correlate with sterilizing immunity generated in HIV-1 vaccinated macaques.
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Heeney JL, Teeuwsen VJ, van Gils M, Bogers WM, De Giuli Morghen C, Radaelli A, Barnett S, Morein B, Akerblom L, Wang Y, Lehner T, and Davis D
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- Animals, Cytokines immunology, HIV-1 physiology, Immunity, Cellular, Macaca mulatta, Neutralization Tests, T-Lymphocytes immunology, T-Lymphocytes virology, AIDS Vaccines administration & dosage, Chemokines, CC immunology, HIV-1 immunology
- Abstract
One of the obstacles to AIDS vaccine development is the variability of HIV-1 within individuals and within infected populations, enabling viral escape from highly specific vaccine induced immune responses. An understanding of the different immune mechanisms capable of inhibiting HIV infection may be of benefit in the eventual design of vaccines effective against HIV-1 variants. To study this we first compared the immune responses induced in Rhesus monkeys by using two different immunization strategies based on the same vaccine strain of HIV-1. We then utilized a chimeric simian/HIV that expressed the envelope of a dual tropic HIV-1 escape variant isolated from a later time point from the same patient from which the vaccine strain was isolated. Upon challenge, one vaccine group was completely protected from infection, whereas all of the other vaccinees and controls became infected. Protected macaques developed highest titers of heterologous neutralizing antibodies, and consistently elevated HIV-1-specific T helper responses. Furthermore, only protected animals had markedly increased concentrations of RANTES, macrophage inflammatory proteins 1alpha and 1beta produced by circulating CD8(+) T cells. These results suggest that vaccine strategies that induce multiple effector mechanisms in concert with beta-chemokines may be desired in the generation of protective immune responses by HIV-1 vaccines.
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- 1998
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15. Cytotoxic T cells and neutralizing antibodies induced in rhesus monkeys by virus-like particle HIV vaccines in the absence of protection from SHIV infection.
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Wagner R, Teeuwsen VJ, Deml L, Notka F, Haaksma AG, Jhagjhoorsingh SS, Niphuis H, Wolf H, and Heeney JL
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- AIDS Vaccines administration & dosage, Animals, Cross Reactions, Macaca mulatta, Reassortant Viruses immunology, Simian Acquired Immunodeficiency Syndrome prevention & control, Virion immunology, AIDS Vaccines immunology, Antibodies, Viral immunology, Cytotoxicity, Immunologic, Simian Acquired Immunodeficiency Syndrome immunology, Simian Immunodeficiency Virus immunology, T-Lymphocytes immunology
- Abstract
HIV Pr55gag has in the absence of other viral components the capacity to self assemble in budding noninfectious virus-like particles (VLP). The immunological spectrum of the HIV-1IIIB gag-derived VLP was expanded either by stable anchoring of chimeric modified gp 120 on the surface of the VLP (type 1) or by replacing sequences of the Pr55gag precursor by the V3 loop and a linear portion of the CD4 binding domain (type 2). This noninfectious antigen delivery system was evaluated for immunogenicity and efficacy in rhesus macaques without adjuvants. Intramuscular immunization with both types of VLP induced high titers of gag-specific antibodies ranging from 1/8000 to 1/510,000 for type 1 VLP and from 1/4000 to 1/16,000 for type 2 VLP. Only animals immunized with type 1 VLP developed substantial endpoint titers of env-specific antibodies (1/2000-1/32,000) with a neutralizing capacity at serum dilutions of 1/32-1/128. Gag- and env-specific cytotoxic T lymphocyte (CTL) activity was induced by both types of VLP at similar levels. Four weeks after the last immunization animals were challenged intravenously with 20 MID50 of the cell free homologous envelope simian/HIV-1IIIB chimeric challenge stock Despite HIV-1-specific neutralizing and CTL responses, all vaccinated animals became infected.
- Published
- 1998
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16. A recombinant prime, peptide boost vaccination strategy can focus the immune response on to more than one epitope even though these may not be immunodominant in the complex immunogen.
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Davis D, Morein B, Akerblom L, Lövgren-Bengtsson K, van Gils ME, Bogers WM, Teeuwsen VJ, and Heeney JL
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- Amino Acid Sequence, Animals, Humans, Immunization, Secondary, Macaca mulatta, Molecular Sequence Data, AIDS Vaccines immunology, Epitopes, HIV Antibodies blood, HIV Envelope Protein gp120 immunology, Vaccines, Synthetic immunology
- Abstract
Rhesus monkeys were successfully vaccinated using a strategy of priming with a candidate envelope subunit vaccine and boosting with synthetic peptides. Priming was carried out with recombinant HIV-1 SF2 envelope glycoprotein incorporated into ISCOMs, following the attachment of a lipid tail. Peptides, covalently linked to ISCOMs, representing linear sequences with the V2 and V3 regions, were used to boost functional antibodies-to neutralizing epitopes in both of these regions. Injections with these peptide formulations substantially increased the titre of serum neutralizing antibodies from low or undetectable levels. In addition to completely neutralizing the homologous HIV-1 SF2 strain, these sera also neutralized the escape variant, HIV-1 SF13. However, no antibodies were boosted which could compete with human, neutralizing monoclonal antibodies recognising conformational epitopes. The peptides also boosted antibodies to a peptide whose sequence lies close to the V2 region neutralizing epitope but does not overlap with it. Importantly, the level of antibodies to an unrelated epitope associated with enhancement of HIV-1 SF13 continued to fall after the peptide boost. Successful protection against challenge with chimeric simian immunodeficiency virus expressing HIV-1 SF13 envelope glycoproteins (SHIV SF13) may be due to an increase in the ratio of neutralizing to enhancing antibodies by selectively boosting with peptides to critical neutralizing epitopes.
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- 1997
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17. A recombinant HIV-1 virus-like particle vaccine: from concepts to a field study.
- Author
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Wagner R, Deml L, Teeuwsen V, Heeney J, Yiming S, and Wolf H
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- Acquired Immunodeficiency Syndrome epidemiology, Acquired Immunodeficiency Syndrome therapy, Animals, China, Humans, Mice, Mice, Inbred BALB C, AIDS Vaccines immunology, AIDS Vaccines therapeutic use, Acquired Immunodeficiency Syndrome immunology, HIV-1 immunology, Vaccines, Synthetic immunology, Vaccines, Synthetic therapeutic use
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- 1996
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18. Safety and immunogenicity of recombinant human immunodeficiency virus-like particles in rodents and rhesus macaques.
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Wagner R, Deml L, Notka F, Wolf H, Schirmbeck R, Reimann J, Teeuwsen V, and Heeney J
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- Animals, Antibody Formation, CD8-Positive T-Lymphocytes immunology, Consumer Product Safety, Epitopes genetics, Gene Expression, Gene Products, gag genetics, HIV genetics, HIV Antibodies immunology, HIV Envelope Protein gp120 genetics, Humans, Immunogenetics, Macaca mulatta, Mice, Mice, Inbred BALB C, Neutralization Tests, Protein Precursors genetics, Recombination, Genetic, T-Lymphocytes, Cytotoxic immunology, Virion immunology, Virion physiology, Epitopes immunology, Gene Products, gag immunology, Genetic Vectors, HIV immunology, Protein Precursors immunology
- Abstract
Data from long-term non-progressing human immunodeficiency virus (HIV)-infected individuals and populations at high risk suggest that an early cytolytic T cell response rather than the humoral immune response might be involved in controlling disease progression. These observations may be used as a guide to the type of response that a vaccine should induce. To clarify the role of different arms of the immune system in conferring protection, the candidate vaccine should allow a regulated, selective induction of different immune responses. Based on a better understanding of the molecular mechanisms regulating the morphogenesis of HIV, we developed an autologous, non-replicating and safe antigen delivery system. This system takes advantage of molecular characteristics of the HIV group-specific antigens (gag) to self-assemble to highly immunogenic virus-like particles (VLP). The immunogenicity of the gag-derived VLP was expanded either by replacing defined domains by selected HIV-1 cytotoxic T lymphocyte (CTL) epitopes (type 1 VLP) or by stable anchoring derivatives of the HIV-1 envelope protein on the surface of the VLP (type 2 VLP). In complete absence of adjuvants, type 1 and type 2 VLP stimulated CD8+ CTL in BALB/c mice, which specifically recognised HIV sequences. In contrast to type 1 VLP, generating an HIV-specific CTL response in the absence of env-specific antibodies, type 2 VLP induced both arms of the immune system including reasonable levels of neutralising antibodies. Initial studies performed in rhesus macaques confirmed these results. Thus, depending on the type and formulation of the VLP, the proposed antigen delivery system allows either the induction of a CTL response (1) in the absence and (2) the presence of an envelope-specific antibody response. A comparison of these approaches in appropriate animal models might contribute to further define the correlates of protection.
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- 1996
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19. Vaccine protection and reduced virus load from heterologous macaque-propagated SIV challenge.
- Author
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Heeney JL, Holterman L, ten Haaft P, Dubbes R, Koornstra W, Teeuwsen V, Bourquin P, Norley S, and Niphuis H
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- Animals, Immunization, Macaca mulatta, Neutralization Tests, Simian Acquired Immunodeficiency Syndrome immunology, Simian Acquired Immunodeficiency Syndrome prevention & control, Simian Acquired Immunodeficiency Syndrome virology, Simian Immunodeficiency Virus isolation & purification, Species Specificity, Time Factors, Vaccines, Attenuated immunology, Vaccines, Synthetic immunology, SAIDS Vaccines immunology, Simian Immunodeficiency Virus immunology
- Abstract
The efficacy of vaccine protection afforded by live attenuated vaccines was tested by heterologous SIVtno8980 challenge following successful protection against homologous SIVmac32H challenge. Animals immunized with the attenuated SIVmacBK28 molecular clone were asymptomatic and virus isolation negative by quantitative virus isolation prior to challenge. Two groups of four animals previously immunized 5 years and 4 months (respectively) were challenged with 100 MID50 of SIVtno8980, as was a third group of four naive controls. All control animals that were challenged developed high levels of plasma antigenemia within 2 weeks of challenge and developed rapid Th/m cell loss whereas vaccinated animals did not. Quantitative virus isolation from peripheral blood mononuclear cells revealed that one of four animals in each group became virus isolation positive but that the virus load in the two vaccinated animals was markedly lower than in nonvaccinated controls. Studies are underway to determine the duration and immunological correlates of protection from AIDS.
- Published
- 1994
20. Antigenic variation of the dominant gp41 epitope in Africa.
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Lange JM, Teeuwsen VJ, Boucher CA, Vahlne A, Barin F, Tjong-A-Hung S, Dekker J, Parkhede U, de Wolf F, and Goudsmit J
- Subjects
- Africa, Amino Acid Sequence, Antigenic Variation, HIV Infections microbiology, HIV-1 genetics, HIV-2 genetics, HIV-2 immunology, Humans, Male, Molecular Sequence Data, Peptides chemical synthesis, Peptides genetics, Peptides immunology, HIV Envelope Protein gp41 genetics, HIV-1 immunology
- Abstract
Objective: To determine the value of (combinations of) synthetic peptides representing immunodominant sites on HIV-1/HIV-2 transmembrane proteins for the detection and discrimination between HIV-1 and HIV-2 infection in various populations., Design and Methods: Two 24-mer synthetic peptides derived from immunodominant sites on the HIV-1 and HIV-2 transmembrane proteins were used separately, in combination (env 1/2), and in combination with recombinant p24 (p24/env) in enzyme-linked immunosorbent assays., Results: Positive reactions with env-1 were found in 150 out of 150 (100%) samples from Dutch AIDS patients, 60 out of 60 (100%) samples from Dutch homosexual men obtained 1 year after HIV-1-antibody seroconversion, 29 out of 30 (96.7%) samples from these men obtained at the time of HIV-1-antibody seroconversion, 40 out of 41 (97.6%) samples from East Africans with AIDS-related symptoms, and three out of 29 (10.3%) samples from West Africans with HIV-2 infection (including a sample from an individual infected with both HIV-1 and HIV-2). Positive reactions with env-2 in these study populations were 11 out of 150 (7.3%), nine out of 60 (15%), none out of 30 (0%), 25 out of 41 (60.9%) and 29 out of 29 (100%), respectively. In the samples with dual reactivity, true versus cross-reactivity could generally be differentiated on the basis of large differences in optical density values in the respective assays. All samples reacted positively with p24/env; 308 out of 310 (99.3%) were positive in the env 1/2 assay. Four East African samples that had negative or only weakly positive reactions with env-1 showed a noticeably stronger reaction with variant peptides derived from Central African isolate sequences. In all samples from HIV-1-infected Dutch homosexual men, the strongest signal was detected using the env-1 peptide sequence, which is derived from European and American isolates., Conclusions: Small peptide antigens may permit the detection of strain-specific antibodies, allowing serological characterization of HIV isolates.
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- 1993
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21. Low number of functionally active B lymphocytes in the peripheral blood of HIV-1-seropositive individuals with low p24-specific serum antibody titers.
- Author
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Teeuwsen VJ, Lange JM, Keet R, Schattenkerk JK, Debouck C, van den Akker R, Goudsmit J, and Osterhaus AD
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- Antibody-Producing Cells immunology, HIV Antibodies blood, HIV Core Protein p24 blood, HIV Core Protein p24 immunology, HIV Envelope Protein gp120 immunology, HIV Reverse Transcriptase, HIV Seropositivity blood, HIV Seropositivity drug therapy, Humans, Immunoglobulin G biosynthesis, In Vitro Techniques, Leukocyte Count, Male, RNA-Directed DNA Polymerase immunology, Zidovudine therapeutic use, B-Lymphocytes immunology, HIV Seropositivity immunology, HIV-1 immunology
- Abstract
The in vitro synthesis of HIV-1, p24-, reverse transcriptase (RT)- and gp120-specific immunoglobulin (Ig) G by unstimulated peripheral blood mononuclear cells (PBMC) from 38 asymptomatic and 10 symptomatic HIV-1-seropositive individuals was analysed. In the majority of these individuals, spontaneous production of HIV-1- and gp120-specific IgG from PBMC cultures was demonstrated. In addition, in the majority of the PBMC cultures from individuals with high serum antibody titers to p24, spontaneous production of p24-specific IgG was shown. In contrast, no p24-specific IgG production was detected in PBMC cultures from seropositive individuals with low or no serum antibody titers to p24. A similar relationship between low or absent RT-specific serum antibody titers and the absence of in vitro RT-specific IgG synthesis was not demonstrated. Furthermore, it was shown that the number of p24-specific B lymphocytes in circulation, as calculated by a spot enzyme-linked immunosorbent assay, were significantly lower in individuals with low serum antibody titers to p24. These results suggest that the decline in p24-specific serum antibodies observed during progression towards AIDS is not merely a reflection of the clearance via immune complexes, but may also be attributable, at least in part, to a reduction of p24-specific antibody-producing active B lymphocytes.
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- 1991
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22. An inhibition enzyme immunoassay using a human monoclonal antibody (K14) reactive with gp41 of HIV-1 for the serology of HIV-1 infections.
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Teeuwsen VJ, Schalken JJ, van der Groen G, van de Akker R, Goudsmit J, and Osterhaus AD
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- Evaluation Studies as Topic, Humans, Antibodies, Monoclonal immunology, HIV Envelope Protein gp41 immunology, HIV Infections diagnosis, HIV-1 immunology, Immunoenzyme Techniques
- Abstract
An inhibition enzyme immunoassay (IEIA), using a human monoclonal antibody (K14) reactive with gp41 of HIV-1, was evaluated for its applicability to the serology of HIV-1 infections. Using panels of serum samples from seronegative and confirmed HIV-1-seropositive individuals, it was shown that all the HIV-1-positive samples in a panel from The Netherlands and 97% of the HIV-1-positive samples from Tanzania were identified by this IEIA. Six per cent of the IEIA-positive samples from Tanzania could not be confirmed in other assays. Testing of serial dilutions of serum samples from African individuals with confirmed HIV-1, HIV-2 or HIV(ANI70) infections in the K14 IEIA, indicated that a HIV-1-specific assay based on this principle may be developed.
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- 1991
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23. Production and characterization of a human monoclonal antibody, reactive with a conserved epitope on gp41 of human immunodeficiency virus type I.
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Teeuwsen VJ, Siebelink KH, Crush-Stanton S, Swerdlow B, Schalken JJ, Goudsmit J, van de Akker R, Stukart MJ, Uytdehaag FG, and Osterhaus AD
- Subjects
- Antibodies, Monoclonal immunology, B-Lymphocytes immunology, Binding, Competitive, Cell Transformation, Viral, Enzyme-Linked Immunosorbent Assay, HIV Antibodies immunology, Herpesvirus 4, Human, Humans, Antibodies, Monoclonal biosynthesis, Epitopes analysis, HIV Antibodies biosynthesis, HIV-1 immunology, Viral Envelope Proteins immunology
- Abstract
A human Epstein-Barr virus-transformed lymphoblastoid B-cell line was generated from peripheral blood mononuclear cells (PBMC) of an asymptomatic human immunodeficiency virus type I (HIV-1) seropositive donor, which produces a human monoclonal antibody K14 (IgG1), reactive with an epitope on the transmembrane part (gp41) of the envelope glycoprotein of HIV-1. This monoclonal antibody reacts with a lysate of HIV-1-infected H9 cells, gradient purified HIV-1, and a vaccinia recombinant HIV-1 gp160 protein, but not with HIV-2 antigens in an enzyme-linked immunosorbent assay (ELISA). When used as an immobilized ligand in an immune affinity column, K14 selectively purifies gp41 from a HIV-1-infected H9 cell lysate. Although no reactivity was observed in ELISA with a panel of partially overlapping synthetic nonapeptides spanning the whole length of HIV-1 gp41, it was shown to react with recombinant envelope proteins, provided that they did contain amino acids 643-692: deletion of this part resulted in the disappearance of the reactivity. Testing of an extensive panel of the sera from HIV-1 seropositive or seronegative donors from Europe and Africa, including a selected group of donors before and after HIV-1 seroconversion, in a competition ELISA with horseradish peroxidase-conjugated K14, showed that the epitope recognized on gp41 is immunodominant and conserved. K14 does not neutralize HIV-1 infectivity or virus-mediated cell fusion, and does not mediate antibody-dependent cellular cytotoxicity.
- Published
- 1990
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24. Impairment of in vitro immune responses occurs within 3 months after HIV-1 seroconversion.
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Teeuwsen VJ, Siebelink KH, de Wolf F, Goudsmit J, UytdeHaag FG, and Osterhaus AD
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- CD4-Positive T-Lymphocytes, Cells, Cultured, Diphtheria Toxoid immunology, Enzyme-Linked Immunosorbent Assay, Gene Products, gag blood, HIV Antibodies biosynthesis, HIV Core Protein p24, Humans, Immunization, Secondary, Immunologic Memory, Leukocyte Count, Leukocytes, Mononuclear immunology, Male, Poliovirus Vaccine, Inactivated immunology, T-Lymphocytes, Regulatory, Tetanus Toxoid immunology, Time Factors, Viral Core Proteins blood, HIV Seropositivity immunology, HIV-1 immunology, Immunoglobulin G biosynthesis, Lymphocyte Activation
- Abstract
In a previous study we have shown that peripheral blood mononuclear cells (PBMC) from asymptomatic HIV-seropositive male homosexuals, who had seroconverted more than 2 years before, were unable to mount a secondary immune response in vitro to certain viral and bacterial antigens. We have extended this study by investigating the secondary immune responses of five male homosexuals, who, by regular screening, were found to have seroconverted for HIV-1 during the preceding 3 months and were subsequently vaccinated with tetanus toxoid and poliovirus vaccine. Six weeks after the booster vaccination, PBMC of the five recently seroconverted individuals were assayed for in vitro mitogen or recall antigen-induced antibody synthesis and lymphocyte proliferation. The results of this study indicate that certain of the in vitro abnormalities of immune reactions, observed in both symptomatic and asymptomatic HIV-seropositive individuals, can already be found within 3 months after seroconversion.
- Published
- 1990
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25. Human anti-idiotypic T lymphocyte clones are activated by autologous anti-rabies virus antibodies presented in association with HLA-DQ molecules.
- Author
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UytdeHaag F, Claassen I, Bunschoten H, Loggen H, Ottenhoff T, Teeuwsen V, and Osterhaus A
- Subjects
- Antibodies, Viral, Antigen-Presenting Cells immunology, CD4-Positive T-Lymphocytes immunology, Clone Cells immunology, HLA-DQ Antigens, Humans, In Vitro Techniques, Lymphocyte Activation, Rabies virus immunology, Receptors, Antigen, T-Cell, Antibodies, Anti-Idiotypic, Immunoglobulin Idiotypes, T-Lymphocytes immunology
- Abstract
The regulatory function of antigen-specific T cells in human antibody responses to protein and carbohydrate determinants of many viral and bacterial antigens has extensively been studied in systems involving in vitro triggering of B cells by antigens or polyclonal activators. Although amply documented in experimental murine models, the existence of T helper cells with receptor specificity for idiotypic determinants of B cell immunoglobulins has not been demonstrated in a human system. We are interested in T helper cell recognition of idiotypic determinants of virus-specific antibody, secreted by human B cells in response to viral antigens, and in the role which such idiotype-specific T helper cells play, alone or in concert with virus-specific T helper cells, to regulate the antibody response. Understanding of the function of different T helper cell subsets in an anti-viral antibody response and especially of the mechanisms of idiotype recognition by T cells is important for the development, and future application in man of idiotype vaccines, the potential of which has been indicated for different pathogens in several animal species. It was realized that for the efficient characterization of each of the T helper cell subsets, the availability of cloned populations of T cells would be inevitable. Furthermore, we argued that if, as predicted by Jerne, idiotype recognizing T helper cells are involved in physiological idiotype regulation in the course of an immune response--e.g., following encounter with virus--cloned populations of T cells should best be obtained by immunization protocols closely mimicking the physiological situation. In a previous report we described the induction of a secondary antibody response in human peripheral blood mononuclear cells (PBMC) in vitro by rabies virus antigen. This response was shown to be T helper cell dependent, and rabies virus-specific T helper cell clones have recently been obtained in our laboratory. The present study describes the generation of cloned lines of anti-idiotypic T4+ cells from rabies virus immune PBMC restimulated with rabies virus antigen in vitro. The cloned T cell lines were found to respond to circulating autologous antibody exhibiting specificity to rabies virus, but not to rabies virus antigen. The clonal proliferation, induced by this "auto-anticlonotypic" antibody, was found to be preceded by modulation of the T3/Ti molecular complex and required presentation of the antibodies by antigen presenting cells in association with HLA-DQ molecules. This observation of MHC-restricted idiotype recognition by human T cells, may have important consequences for the understanding of the regulation of th
- Published
- 1987
26. Cloning of a third nitrate reductase gene from the cyanobacterium Anacystis nidulans R2 using a shuttle cosmid library.
- Author
-
Kuhlemeier CJ, Teeuwsen VJ, Janssen MJ, and van Arkel GA
- Subjects
- Bacteriophage lambda genetics, Cloning, Molecular methods, Cyanobacteria enzymology, DNA, Bacterial genetics, DNA, Recombinant, Escherichia coli genetics, Genes, Genetic Complementation Test, Genetic Vectors, Plasmids, Bacterial Proteins genetics, Cyanobacteria genetics, Genes, Bacterial, Nitrate Reductases genetics
- Abstract
A strategy for gene cloning in the cyanobacterium Anacystis nidulans R2 was developed which made use of a gene library constructed in a shuttle cosmid vector. The method involved phenotypic complementation of mutants with pooled cosmid DNA. The development of the procedure and its application to the cloning of a third gene involved in nitrate reduction are described.
- Published
- 1984
- Full Text
- View/download PDF
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