1. Reverse Genetic Assessment of the Roles Played by the Spike Protein and ORF3 in Porcine Epidemic Diarrhea Virus Pathogenicity.
- Author
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Kristen-Burmann, Claudia, Rogger, Peter, Veiga, Inês Berenguer, Riebesehl, Stefanie, Rappe, Julie, Ebert, Nadine, Sautter, Carmen A., Kelly, Jenna N., Stalder, Hanspeter, Ehmann, Rosina, Huber, Michael, Posthaus, Horst, Ruggli, Nicolas, Thiel, Volker, and Tekesa, Gergely
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PORCINE epidemic diarrhea virus , *CORONAVIRUSES , *REVERSE genetics , *GENETIC vectors , *VACCINIA , *ARTIFICIAL chromosomes , *MOLECULAR cloning - Abstract
Porcine epidemic diarrhea virus is a swine pathogen that has been responsible for significant animal and economic losses worldwide in recent years. In this manuscript, we report the generation of a reverse genetics system C(RGS) for the highly virulent US PEDV strain Minnesota (PEDV-MN; GenBank accession number KF468752), which was based on the assembly and cloning of synthetic DNA, using vaccinia virus as a cloning vector. Viral rescue was only possible following the substitution of 2 nucleotides within the 59UTR and 2 additional nucleotides within the spike gene, based on the sequence of the cell culture-adapted strains. Besides displaying a highly pathogenic phenotype in newborn piglets, in comparison with the parental virus, the rescued recombinant PEDVMN was used to confirm that the PEDV spike gene has an important role in PEDV virulence and that the impact of an intact PEDV ORF3 on viral pathogenicity is modest. Moreover, a chimeric virus with a TGEV spike gene in the PEDV backbone generated with RGS was able to replicate efficiently in vivo and could be readily transmitted between piglets. Although this chimeric virus did not cause severe disease upon the initial infection of piglets, there was evidence of increasing pathogenicity upon transmission to contact piglets. The RGS described in this study constitutes a powerful tool with which to study PEDV pathogenesis and can be used to generate vaccines against porcine enteric coronaviruses. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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