280 results on '"Temmam, Sarah"'
Search Results
2. Virological characteristics of a SARS-CoV-2-related bat coronavirus, BANAL-20-236
- Author
-
Sawa, Hirofumi, Mizuma, Keita, Li, Jingshu, Mimura, Yume, Ohari, Yuma, Tsubo, Tomoya, Ferdous, Zannatul, Shishido, Kenji, Mohri, Hiromi, Iida, Miki, Tsujino, Shuhei, Misawa, Naoko, Usui, Kaoru, Saikruang, Wilaiporn, Lytras, Spyridon, Kawakubo, Shusuke, Nishumura, Luca, Mendoza Tolentino, Jarel Elgin, Li, Wenye, Yo, Maximilian Stanley, Horinaka, Kio, Suganami, Mai, Chiba, Mika, Yoshimura, Ryo, Yasuda, Kyoko, Iida, Keiko, Strange, Adam Patrick, Ohsumi, Naomi, Tanaka, Shiho, Ogawa, Eiko, Okumura, Kaho, Fukuda, Tsuki, Osujo, Rina, Yoshida, Isao, Nakagawa, So, Takaori-Kondo, Akifumi, Shirakawa, Kotaro, Nagata, Kayoko, Nomura, Ryosuke, Horisawa, Yoshihito, Tashiro, Yusuke, Kawai, Yugo, Nakata, Yoshitaka, Futatsusako, Hiroki, Sakamoto, Ayaka, Yasuhara, Naoko, Hashiguchi, Takao, Suzuki, Tateki, Kimura, Kanako, Sasaki, Jiei, Nakajima, Yukari, Yajima, Hisano, Irie, Takashi, Kawabata, Ryoko, Sasaki-Tabata, Kaori, Shimizu, Ryo, Monira Begum, M.S.T., Jonathan, Michael, Mugita, Yuka, Leong, Sharee, Takahashi, Otowa, Ichihara, Kimiko, Ueno, Takamasa, Motozono, Chihiro, Toyoda, Mako, Saito, Akatsuki, Kosaka, Anon, Kawano, Miki, Matsubara, Natsumi, Nishiuchi, Tomoko, Zahradnik, Jiri, Andrikopoulos, Prokopios, Padilla-Blanco, Miguel, Konar, Aditi, Fujita, Shigeru, Plianchaisuk, Arnon, Deguchi, Sayaka, Ito, Hayato, Nao, Naganori, Wang, Lei, Nasser, Hesham, Tamura, Tomokazu, Kimura, Izumi, Kashima, Yukie, Suzuki, Rigel, Suzuki, Saori, Kida, Izumi, Tsuda, Masumi, Oda, Yoshitaka, Hashimoto, Rina, Watanabe, Yukio, Uriu, Keiya, Yamasoba, Daichi, Guo, Ziyi, Hinay, Alfredo A., Jr., Kosugi, Yusuke, Chen, Luo, Pan, Lin, Kaku, Yu, Chu, Hin, Donati, Flora, Temmam, Sarah, Eloit, Marc, Yamamoto, Yuki, Nagamoto, Tetsuharu, Asakura, Hiroyuki, Nagashima, Mami, Sadamasu, Kenji, Yoshimura, Kazuhisa, Suzuki, Yutaka, Ito, Jumpei, Ikeda, Terumasa, Tanaka, Shinya, Matsuno, Keita, Fukuhara, Takasuke, Takayama, Kazuo, and Sato, Kei
- Published
- 2024
- Full Text
- View/download PDF
3. SARS‐CoV‐2‐related bat virus behavior in human‐relevant models sheds light on the origin of COVID‐19
- Author
-
Temmam, Sarah, Montagutelli, Xavier, Herate, Cécile, Donati, Flora, Regnault, Béatrice, Attia, Mikael, Baquero Salazar, Eduard, Chretien, Delphine, Conquet, Laurine, Jouvion, Grégory, Pipoli Da Fonseca, Juliana, Cokelaer, Thomas, Amara, Faustine, Relouzat, Francis, Naninck, Thibaut, Lemaitre, Julien, Derreudre‐Bosquet, Nathalie, Pascal, Quentin, Bonomi, Massimiliano, Bigot, Thomas, Munier, Sandie, Rey, Felix A, Le Grand, Roger, van der Werf, Sylvie, and Eloit, Marc
- Published
- 2023
- Full Text
- View/download PDF
4. Bat coronaviruses related to SARS-CoV-2 and infectious for human cells
- Author
-
Temmam, Sarah, Vongphayloth, Khamsing, Baquero, Eduard, Munier, Sandie, Bonomi, Massimiliano, Regnault, Béatrice, Douangboubpha, Bounsavane, Karami, Yasaman, Chrétien, Delphine, Sanamxay, Daosavanh, Xayaphet, Vilakhan, Paphaphanh, Phetphoumin, Lacoste, Vincent, Somlor, Somphavanh, Lakeomany, Khaithong, Phommavanh, Nothasin, Pérot, Philippe, Dehan, Océane, Amara, Faustine, Donati, Flora, Bigot, Thomas, Nilges, Michael, Rey, Félix A., van der Werf, Sylvie, Brey, Paul T., and Eloit, Marc
- Published
- 2022
- Full Text
- View/download PDF
5. Virological characteristics of a SARS-CoV-2-related bat coronavirus, BANAL-20-236
- Author
-
Fujita, Shigeru, primary, Plianchaisuk, Arnon, additional, Deguchi, Sayaka, additional, Ito, Hayato, additional, Nao, Naganori, additional, Wang, Lei, additional, Nasser, Hesham, additional, Tamura, Tomokazu, additional, Kimura, Izumi, additional, Kashima, Yukie, additional, Suzuki, Rigel, additional, Suzuki, Saori, additional, Kida, Izumi, additional, Tsuda, Masumi, additional, Oda, Yoshitaka, additional, Hashimoto, Rina, additional, Watanabe, Yukio, additional, Uriu, Keiya, additional, Yamasoba, Daichi, additional, Guo, Ziyi, additional, Hinay, Alfredo A., additional, Kosugi, Yusuke, additional, Chen, Luo, additional, Pan, Lin, additional, Kaku, Yu, additional, Chu, Hin, additional, Donati, Flora, additional, Temmam, Sarah, additional, Eloit, Marc, additional, Yamamoto, Yuki, additional, Nagamoto, Tetsuharu, additional, Asakura, Hiroyuki, additional, Nagashima, Mami, additional, Sadamasu, Kenji, additional, Yoshimura, Kazuhisa, additional, Suzuki, Yutaka, additional, Sawa, Hirofumi, additional, Mizuma, Keita, additional, Li, Jingshu, additional, Mimura, Yume, additional, Ohari, Yuma, additional, Tsubo, Tomoya, additional, Ferdous, Zannatul, additional, Shishido, Kenji, additional, Mohri, Hiromi, additional, Iida, Miki, additional, Tsujino, Shuhei, additional, Misawa, Naoko, additional, Usui, Kaoru, additional, Saikruang, Wilaiporn, additional, Lytras, Spyridon, additional, Kawakubo, Shusuke, additional, Nishumura, Luca, additional, Mendoza Tolentino, Jarel Elgin, additional, Li, Wenye, additional, Yo, Maximilian Stanley, additional, Horinaka, Kio, additional, Suganami, Mai, additional, Chiba, Mika, additional, Yoshimura, Ryo, additional, Yasuda, Kyoko, additional, Iida, Keiko, additional, Strange, Adam Patrick, additional, Ohsumi, Naomi, additional, Tanaka, Shiho, additional, Ogawa, Eiko, additional, Okumura, Kaho, additional, Fukuda, Tsuki, additional, Osujo, Rina, additional, Yoshida, Isao, additional, Nakagawa, So, additional, Takaori-Kondo, Akifumi, additional, Shirakawa, Kotaro, additional, Nagata, Kayoko, additional, Nomura, Ryosuke, additional, Horisawa, Yoshihito, additional, Tashiro, Yusuke, additional, Kawai, Yugo, additional, Nakata, Yoshitaka, additional, Futatsusako, Hiroki, additional, Sakamoto, Ayaka, additional, Yasuhara, Naoko, additional, Hashiguchi, Takao, additional, Suzuki, Tateki, additional, Kimura, Kanako, additional, Sasaki, Jiei, additional, Nakajima, Yukari, additional, Yajima, Hisano, additional, Irie, Takashi, additional, Kawabata, Ryoko, additional, Sasaki-Tabata, Kaori, additional, Shimizu, Ryo, additional, Monira Begum, M.S.T., additional, Jonathan, Michael, additional, Mugita, Yuka, additional, Leong, Sharee, additional, Takahashi, Otowa, additional, Ichihara, Kimiko, additional, Ueno, Takamasa, additional, Motozono, Chihiro, additional, Toyoda, Mako, additional, Saito, Akatsuki, additional, Kosaka, Anon, additional, Kawano, Miki, additional, Matsubara, Natsumi, additional, Nishiuchi, Tomoko, additional, Zahradnik, Jiri, additional, Andrikopoulos, Prokopios, additional, Padilla-Blanco, Miguel, additional, Konar, Aditi, additional, Ito, Jumpei, additional, Ikeda, Terumasa, additional, Tanaka, Shinya, additional, Matsuno, Keita, additional, Fukuhara, Takasuke, additional, Takayama, Kazuo, additional, and Sato, Kei, additional
- Published
- 2024
- Full Text
- View/download PDF
6. Preschool-age children maintain a distinct memory CD4+ T cell and memory B cell response after SARS-CoV-2 infection.
- Author
-
Manfroi, Benoît, Cuc, Bui Thi, Sokal, Aurélien, Vandenberghe, Alexis, Temmam, Sarah, Attia, Mikaël, El Behi, Mohamed, Camaglia, Francesco, Nguyen, Ngan Thu, Pohar, Jelka, Salem-Wehbe, Layale, Pottez-Jouatte, Valentine, Borzakian, Sibyline, Elenga, Narcisse, Galeotti, Caroline, Morelle, Guillaume, de truchis de Lays, Camille, Semeraro, Michaela, Romain, Anne-Sophie, and Aubart, Mélodie
- Subjects
SARS-CoV-2 ,CYTOTOXIC T cells ,IMMUNOLOGIC memory ,T cells ,AGE groups ,CORONAVIRUS diseases - Abstract
The development of the human immune system lasts for several years after birth. The impact of this maturation phase on the quality of adaptive immunity and the acquisition of immunological memory after infection at a young age remains incompletely defined. Here, using an antigen-reactive T cell (ARTE) assay and multidimensional flow cytometry, we profiled circulating severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)–reactive CD3
+ CD4+ CD154+ T cells in children and adults before infection, during infection, and 11 months after infection, stratifying children into separate age groups and adults according to disease severity. During SARS-CoV-2 infection, children younger than 5 years old displayed a lower antiviral CD4+ T cell response, whereas children older than 5 years and adults with mild disease had, quantitatively and phenotypically, comparable virus-reactive CD4+ T cell responses. Adults with severe disease mounted a response characterized by higher frequencies of virus-reactive proinflammatory and cytotoxic T cells. After SARS-CoV-2 infection, preschool-age children not only maintained neutralizing SARS-CoV-2–reactive antibodies postinfection comparable to adults but also had phenotypically distinct memory T cells displaying high inflammatory features and properties associated with migration toward inflamed sites. Moreover, preschool-age children had markedly fewer circulating virus-reactive memory B cells compared with the other cohorts. Collectively, our results reveal unique facets of antiviral immunity in humans at a young age and indicate that the maturation of adaptive responses against SARS-CoV-2 toward an adult-like profile occurs in a progressive manner. Editor's summary: The immune system continues to develop throughout childhood, and it is established that children have distinct immune responses to vaccines and infections as compared with adults. Here, Manfroi et al. characterized the immune response to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in children. The authors found that preschool-age children had a different adaptive immune response, with a lower frequency of antiviral CD4+ T cells, compared with older children and adults; this younger cohort also developed phenotypically distinct memory T and B cell responses after recovery compared with the other cohorts. This study highlights that responses to SARS-CoV-2 infection in young children are unique and reflect the ongoing maturation of the immune system into adulthood. —Courtney Malo [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
7. Author Correction: Bat coronaviruses related to SARS-CoV-2 and infectious for human cells
- Author
-
Temmam, Sarah, Vongphayloth, Khamsing, Baquero, Eduard, Munier, Sandie, Bonomi, Massimiliano, Regnault, Béatrice, Douangboubpha, Bounsavane, Karami, Yasaman, Chrétien, Delphine, Sanamxay, Daosavanh, Xayaphet, Vilakhan, Paphaphanh, Phetphoumin, Lacoste, Vincent, Somlor, Somphavanh, Lakeomany, Khaithong, Phommavanh, Nothasin, Pérot, Philippe, Dehan, Océane, Amara, Faustine, Donati, Flora, Bigot, Thomas, Nilges, Michael, Rey, Félix A., van der Werf, Sylvie, Brey, Paul T., and Eloit, Marc
- Published
- 2022
- Full Text
- View/download PDF
8. Diversity of Viruses in Ixodes ricinus in Europe including Novel and Potential Arboviruses
- Author
-
Bratuleanu, Bianca Elena, primary, Răileanu, Cristian, additional, Bennouna, Amal, additional, Chretien, Delphine, additional, Bigot, Thomas, additional, Guardado-Calvo, Pablo, additional, Savuta, Gheorghe, additional, Moutailler, Sara, additional, Eloit, Marc, additional, and Temmam, Sarah, additional
- Published
- 2023
- Full Text
- View/download PDF
9. Genotype and Phenotype Characterization of Rhinolophus sp. Sarbecoviruses from Vietnam: Implications for Coronavirus Emergence
- Author
-
Temmam, Sarah, primary, Tu, Tran Cong, additional, Regnault, Béatrice, additional, Bonomi, Massimiliano, additional, Chrétien, Delphine, additional, Vendramini, Léa, additional, Duong, Tran Nhu, additional, Phong, Tran Vu, additional, Yen, Nguyen Thi, additional, Anh, Hoang Ngoc, additional, Son, Tran Hai, additional, Anh, Pham Tuan, additional, Amara, Faustine, additional, Bigot, Thomas, additional, Munier, Sandie, additional, Thong, Vu Dinh, additional, van der Werf, Sylvie, additional, Nam, Vu Sinh, additional, and Eloit, Marc, additional
- Published
- 2023
- Full Text
- View/download PDF
10. Comprehensive Characterization of Viral Diversity of Female Mosquitoes in Madagascar
- Author
-
Bennouna, Amal, primary, Tantely, Michael Luciano, additional, Raharinosy, Vololoniaina, additional, Andriamandimby, Soa Fy, additional, Bigot, Thomas, additional, Chrétien, Delphine, additional, Jacquemet, Elise, additional, Volant, Stevenn, additional, Temmam, Sarah, additional, Dussart, Philippe, additional, Lacoste, Vincent, additional, Girod, Romain, additional, and Eloit, Marc, additional
- Published
- 2023
- Full Text
- View/download PDF
11. Longitudinal Study of Viral Diversity Associated with Mosquito Species Circulating in Cambodia
- Author
-
Mohamed Ali, Souand, primary, Rakotonirina, Antsa, additional, Heng, Kimly, additional, Jacquemet, Elise, additional, Volant, Stevenn, additional, Temmam, Sarah, additional, Boyer, Sebastien, additional, and Eloit, Marc, additional
- Published
- 2023
- Full Text
- View/download PDF
12. Acute tubulointerstitial nephritis with or without uveitis: a novel form of post-acute COVID-19 syndrome in children
- Author
-
Avramescu, Marina, primary, Isnard, Pierre, additional, Temmam, Sarah, additional, Chevalier, Agnès, additional, Bastard, Paul, additional, Attia, Mikael, additional, Berthaud, Romain, additional, Fila, Marc, additional, Dossier, Claire, additional, Hogan, Julien, additional, Ulinski, Tim, additional, Leguevaques, Damia, additional, Louillet, Férielle, additional, Casado, Edouard Martinez, additional, Halimi, Jean-Michel, additional, Cloarec, Sylvie, additional, Zaloszyc, Ariane, additional, Faudeux, Camille, additional, Rousset-Rouvière, Caroline, additional, Clavé, Stéphanie, additional, Harambat, Jérôme, additional, Rollot, Edouard, additional, Simon, Thomas, additional, Nallet-Amate, Megan, additional, Ranchin, Bruno, additional, Bacchetta, Justine, additional, Porcheret, Florence, additional, Bernard, Josselin, additional, Ryckewaert, Amélie, additional, Jamet, Anne, additional, Fourgeaud, Jacques, additional, Da Rocha, Nicolas, additional, Pérot, Philippe, additional, Kuperwasser, Nicolas, additional, Bouazza, Naïm, additional, Rabant, Marion, additional, Duong Van Huyen, Jean-Paul, additional, Robert, Matthieu P., additional, Zuber, Julien, additional, Casanova, Jean-Laurent, additional, Eloit, Marc, additional, Sermet-Gaudelus, Isabelle, additional, and Boyer, Olivia, additional
- Published
- 2023
- Full Text
- View/download PDF
13. Virus Pop—Expanding Viral Databases by Protein Sequence Simulation
- Author
-
Kende, Julia, primary, Bonomi, Massimiliano, additional, Temmam, Sarah, additional, Regnault, Béatrice, additional, Pérot, Philippe, additional, Eloit, Marc, additional, and Bigot, Thomas, additional
- Published
- 2023
- Full Text
- View/download PDF
14. Protocol for Generating Infectious RNA Viromes from Complex Biological Samples
- Author
-
Monteil-Bouchard, Sonia, primary, Temmam, Sarah, additional, and Desnues, Christelle, additional
- Published
- 2018
- Full Text
- View/download PDF
15. A Search for Tick-Associated, Bronnoya-like Virus Spillover into Sheep
- Author
-
Bratuleanu, Bianca Elena, primary, Raileanu, Cristian, additional, Chrétien, Delphine, additional, Guardado-Calvo, Pablo, additional, Bigot, Thomas, additional, Savuta, Gheorghe, additional, Temmam, Sarah, additional, and Eloit, Marc, additional
- Published
- 2023
- Full Text
- View/download PDF
16. De nouveaux coronavirus de chauve-souris similaires à SARS-CoV-2 éclairent l'origine de la pandémie de COVID-19
- Author
-
Temmam, Sarah, Vongphayloth, Khamsing, Baquero Salazar, Eduard, Munier, Sandie, Bonomi, Massimiliano, Regnault, Béatrice, Douangboubpha, Bounsavane, Karami, Yasaman, Chrétien, Delphine, Sanamxay, Daosavanh, Xayaphet, Vilakhan, Paphaphanh, Phetphoumin, Lacoste, Vincent, Somlor, Somphavanh, Lakeomany, Khaithong, Phommavanh, Nothasin, Pérot, Philippe, Dehan, Océane, Amara, Faustine, Donati, Flora, Bigot, Thomas, Nilges, Michael, Rey, Félix, van Der Werf, Sylvie, Brey, Paul, Eloit, Marc, Découverte de pathogènes – Pathogen discovery, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Centre Collaborateur de l'OIE de Détection et identification chez l’homme des pathogènes animaux émergents et développement d’outils pour leur diagnostic / Collaborating Center for the Detection and identification in humans of emerging animal pathogens and development of tools for their diagnoses (CCOIE-OIECC), Institut Pasteur [Paris] (IP)-Organisation Mondiale de la Santé Animale / World Organisation Animal Health [Paris] (OIE)-Université Paris Cité (UPCité), Institut Pasteur du Laos, Réseau International des Instituts Pasteur (RIIP), Virologie Structurale - Structural Virology, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses (GMV-ARN (UMR_3569 / U-Pasteur_2)), Bioinformatique structurale - Structural Bioinformatics, National University of Laos, Centre National de Référence des virus des infections respiratoires (dont la grippe) - National Reference Center Virus Influenzae [Paris] (CNR - laboratoire coordonnateur), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, and TEMMAM, Sarah
- Subjects
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,[SDE.BE] Environmental Sciences/Biodiversity and Ecology ,pandémie ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,coronavirus ,chauve-souris ,[SDE.BE]Environmental Sciences/Biodiversity and Ecology ,ComputingMilieux_MISCELLANEOUS - Abstract
International audience
- Published
- 2022
17. Development of real-time RT-PCR for the detection of low concentrations of Rift Valley fever virus
- Author
-
Maquart, Marianne, Temmam, Sarah, Héraud, Jean-Michel, Leparc-Goffart, Isabelle, Cêtre-Sossah, Catherine, Dellagi, Koussay, Cardinale, Eric, and Pascalis, Hervé
- Published
- 2014
- Full Text
- View/download PDF
18. Microseek: A Protein-Based Metagenomic Pipeline for Virus Diagnostic and Discovery
- Author
-
Pérot, Philippe, primary, Bigot, Thomas, additional, Temmam, Sarah, additional, Regnault, Béatrice, additional, and Eloit, Marc, additional
- Published
- 2022
- Full Text
- View/download PDF
19. Seroprevalence of Crimean-Congo Hemorrhagic Fever Among Small Ruminants from Southern Romania
- Author
-
Bratuleanu, Bianca, primary, Anita, Adriana, additional, Temmam, Sarah, additional, Dascalu, Anca, additional, Crivei, Luciana, additional, Cozma, Andreea, additional, Pourquier, Philippe, additional, Savuta, Gheorghe, additional, Eloit, Marc, additional, and Anita, Dragos, additional
- Published
- 2022
- Full Text
- View/download PDF
20. SARS-CoV-2-Related Bat Virus in Human Relevant Models Sheds Light on the Proximal Origin of COVID-19
- Author
-
Temmam, Sarah, primary, Montagutelli, Xavier, additional, Hérate, Cécile, additional, Donati, Flora, additional, Regnault, Beatrice, additional, Attia, Mikaël, additional, Salazar, Eduard Baquero, additional, Chrétien, Delphine, additional, Conquet, Laurine, additional, Jouvion, Grégory, additional, Fonseca, Juliana Pipoli da, additional, Cokelaer, Thomas, additional, Amara, Faustine, additional, Relouzat, Francis, additional, Naninck, Thibaut, additional, Lemaitre, Julien, additional, Dereuddre-Bosquet, Nathalie, additional, Pascal, Quentin, additional, Bonomi, Max, additional, Bigot, Thomas, additional, Munier, Sandie, additional, Rey, Félix, additional, Grand, Roger Le, additional, Werf, Sylvie van der, additional, and Eloit, Marc, additional
- Published
- 2022
- Full Text
- View/download PDF
21. MO1039: 1-Year Follow-Up Data of Arterial Abnormalities Identified in Kidneys Transplanted into Children During the First Covid-19 Pandemic Wave
- Author
-
Grapin, Mathilde, primary, Berteloot, Laureline, additional, Berthaud, Romain, additional, Temmam, Sarah, additional, Blanc, Thomas, additional, Charbit, Marina, additional, Pastural, Myriam, additional, Eloit, Marc, additional, Sermet-Gaudelus, Isabelle, additional, Dehoux, Laurène, additional, and Boyer, Olivia, additional
- Published
- 2022
- Full Text
- View/download PDF
22. Detection of Phenuiviridae, Chuviridae Members, and a Novel Quaranjavirus in Hard Ticks From Danube Delta
- Author
-
Bratuleanu, Bianca Elena, primary, Temmam, Sarah, additional, Munier, Sandie, additional, Chrétien, Delphine, additional, Bigot, Thomas, additional, van der Werf, Sylvie, additional, Savuta, Gheorghe, additional, and Eloit, Marc, additional
- Published
- 2022
- Full Text
- View/download PDF
23. Faune sauvage et SARS-CoV-2 : exposition des animaux de zoo pendant la circulation du virus en France en 2020
- Author
-
Temmam, Sarah, Lécu, Alexis, Sailler, Anaïs, Lamgait, Benjamin, Ollivet-Courtois, Florence, Huon, Christèle, Behilill, Sylvie, Vanhomwegen, Jessica, Caro, Valérie, Manuguerra, Jean-Claude, van Der Werf, Sylvie, Eloit, Marc, TEMMAM, Sarah, Découverte de pathogènes – Pathogen discovery, Institut Pasteur [Paris] (IP), Muséum national d'Histoire naturelle (MNHN), Parc zoologique de Paris, Fondation française pour la recherche sur la biodiversité, ZooParc de Beauval, Beauval Nature, Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses (GMV-ARN (UMR_3569 / U-Pasteur_2)), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Centre National de Référence des virus des infections respiratoires (dont la grippe) - National Reference Center Virus Influenzae [Paris] (CNR - laboratoire coordonnateur), Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Cellule d'Intervention Biologique d'Urgence (Centre National de Référence) - Laboratory for Urgent Response to Biological Threats (National Reference Center) (CIBU), Université Paris Cité (UPCité)-Environnement et Risques infectieux - Environment and Infectious Risks (ERI), Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité)-Institut Pasteur [Paris] (IP), Environnement et Risques infectieux - Environment and Infectious Risks (ERI), and École nationale vétérinaire - Alfort (ENVA)
- Subjects
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,[SDV.BA.MVSA] Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health - Abstract
International audience; Contexte : Le virus SARS-CoV-2 responsable de la pandémie de COVID-19 a causé à ce jour plus de 88 millions d’infections et 1,9 millions de décès. Comme pour les autres coronavirus, l’infection chez l’homme provient probablement d’une transgression de barrière d’espèce depuis un réservoir animal, vraisemblablement une chauve-souris, peut-être via un hôte intermédiaire. Au fur et à mesure de la diffusion du virus dans le monde, des infections anthropozoonotiques occasionnelles d'animaux ont été signalées, chez des animaux domestiques (chats, chiens) et d’élevage (visons) puis dans la faune sauvage captive des zoos (gorilles, tigres, lions, puma, panthère des neige) via des contacts étroits avec des humains a- ou pauci-symptomatiques. Des infections expérimentales et modélisations bio-informatiques ont également démontré ou suggéré la sensibilité d’autres espèces à l’infection par SARS-CoV-2, notamment des primates non-humains. L’objectif du travail était d’identifier des cas d’infection à SARS-CoV-2 chez des animaux sauvages en captivité.Méthodes : D’avril à novembre 2020, des prélèvements sanguins et des écouvillons nasaux-pharyngés et rectaux ont été effectués sur 177 animaux sauvages provenant de zoos et d’élevages français, dont des chiroptères, des félins et des primates. Le critère principal d’inclusion était la confirmation d’au moins un cas de SARS-CoV-2 dans le personnel animalier des établissements. La recherche du génome de SARS-CoV-2 a été effectuée par une RT-PCR en temps réel en duplex ciblant le gène de la polymérase selon la méthode développée par le CNR des virus respiratoires ; et le séquençage des génomes complets a été réalisé avec le séquenceur MinION selon le protocole ARTIC. Le statut sérologique des animaux a été évalué par la technique de LIPS (Luciférase Immuno-Precipitation System) ciblant les domaines S1, S2, S, et RBD de la protéine de spicule, et le domaine C-terminal de la nucléoprotéine N. Résultats : Le génome de SARS-CoV-2 a été détecté dans 3 écouvillons nasaux-pharyngés provenant de 2 lions vivant dans le même enclos au sein du zoo A. Ces animaux étaient asymptomatiques avant et après le prélèvement. Le séquençage complet de ces virus a révélé que ces souches appartenaient au lignage B.1.202, qui a circulé de mars à mai 2020 principalement en Australie et aux USA. Sur 145 sérums analysés (comprenant des Canidae, des chiroptères, des félins, des Herpestidae, des Mustelidae, des Périssodactyles, des primates et prosimiens, et un Ursidae), la séroconversion de 3 tigres et de 3 lions provenant du site B a été détectée entre avril et octobre 2020. Ces félidés n’ont jamais présenté de signe clinique. En revanche, un babouin du zoo C a également été testé transitoirement séropositif, mais négatif en séroneutralisation. Lors de son anesthésie 3 mois après, l’animal présentait des images radiographiques compatibles avec une pneumopathie mais un résultat sérologique devenu négatif et une absence de détection antigénique ou PCR sur les prélèvements bronchiques effectués.Conclusion : L’infection d’animaux sauvages par un SARS-CoV-2 d’origine humaine est peu fréquente mais possible ; le maintien constant de précautions sanitaires est donc indispensable afin de protéger ces espèces à haute valeur de conservation.
- Published
- 2021
24. Arterial abnormalities identified in kidneys transplanted into children during the COVID-19 pandemic
- Author
-
Berteloot, Laureline, Berthaud, Romain, Temmam, Sarah, Lozach, Cécile, Zanelli, Elisa, Blanc, Thomas, Heloury, Yves, Capito, Carmen, Chardot, Christophe, Sarnacki, Sabine, Garcelon, Nicolas, Lacaille, Florence, Charbit, Marina, Pastural, Myriam, Rabant, Marion, Boddaert, Nathalie, Leruez-Ville, Marianne, Eloit, Marc, Sermet-Gaudelus, Isabelle, Dehoux, Laurène, and Boyer, Olivia
- Published
- 2021
- Full Text
- View/download PDF
25. Development and validation of real-time one-step reverse transcription-PCR for the detection and typing of dengue viruses
- Author
-
Leparc-Goffart, Isabelle, Baragatti, Meili, Temmam, Sarah, Tuiskunen, Anne, Moureau, Gregory, Charrel, Rémi, and de Lamballerie, Xavier
- Published
- 2009
- Full Text
- View/download PDF
26. Une infection antérieure par des coronavirus saisonniers n’empêche pas la COVID-19 chez les enfants
- Author
-
Sermet-Gaudelus, Isabelle, Temmam, Sarah, Huon, Christèle, Behillil, Sylvie, Gajdos, Vincent, Bigot, Thomas, Lurier, Thibaut, Chrétien, Delphine, Backovic, Marija, Delaunay-Moisan, Agnès, Donati, Flora, Albert, Mélanie, Foucaud, Elsa, Mesplées, Bettina, Benoist, Grégoire, Faye, Albert, Duval-Arnould, Marc, Cretolle, Célia, Charbit, Marina, Aubart, Mélodie, Auriau, Johanne, Lorrot, Mathie, Kariyawasam, Dulanjalee, Fertitta, Laura, Orliaguet, Gilles, Pigneur, Bénédicte, Bader-Meunier, Brigitte, Briand, Coralie, Enouf, Vincent, Toubiana, Julie, Guilleminot, Tiffany, van Der Werf, Sylvie, Leruez-Ville, Marianne, Eloit, Marc, Découverte de pathogènes – Pathogen discovery, Institut Pasteur [Paris] (IP), Génétique Moléculaire des Virus à ARN - Molecular Genetics of RNA Viruses (GMV-ARN (UMR_3569 / U-Pasteur_2)), Institut Pasteur [Paris] (IP)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Centre National de Référence des virus des infections respiratoires (dont la grippe) - National Reference Center Virus Influenzae [Paris] (CNR - laboratoire coordonnateur), AP-HP - Hôpital Antoine Béclère [Clamart], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Unité Mixte de Recherche d'Épidémiologie des maladies Animales et zoonotiques (UMR EPIA), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Virologie Structurale - Structural Virology, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Département Plateforme (PF I2BC), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Hôpital Jean Verdier [AP-HP], Hôpital Louis Mourier - AP-HP [Colombes], Hôpital Ambroise Paré [AP-HP], Hôpital Robert Debré, Epidémiologie Clinique et Evaluation Economique Appliquées aux Populations Vulnérables (ECEVE (U1123 / UMR_S_1123)), Institut National de la Santé et de la Recherche Médicale (INSERM)-AP-HP Hôpital universitaire Robert-Debré [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Cité (UPCité), AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre), CHU Necker - Enfants Malades [AP-HP], Imagine - Institut des maladies génétiques (IHU) (Imagine - U1163), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Service de neurochirurgie pédiatrique [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Plateforme de Microbiologie Mutualisée (PIBnet) - Mutualized Platform for Microbiology (P2M), Biodiversité et Epidémiologie des Bactéries pathogènes - Biodiversity and Epidemiology of Bacterial Pathogens, École nationale vétérinaire - Alfort (ENVA), Institut Pasteur, ANR, Commission européenne, CRNS, Université Paris Cité, Santé publique France, and ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010)
- Subjects
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology - Abstract
International audience; Justification: Les enfants présentent un faible taux de COVID-19 et de syndrome inflammatoire multisystémique sévère apparenté à la maladie de Kawasaki (MIS) mais présentent une prévalence élevée d'infections aux coronavirus saisonniers.Objectif: Nous avons évalué par sérologie si une infection antérieure par les coronavirus saisonniers (NL63, HKU1, 229E, OC43) pouvait fournir une immunité croisée contre l'infection par le SARS-CoV-2 chez les enfants.Méthodes: Une étude observationnelle multicentrique transversale a été mise en place chez i) des enfants pauci- ou asymptomatiques hospitalisés à Paris lors de la première vague pour des raisons autres qu’une infection à SARS-CoV-2 (groupe HOS, n = 739) ; et ii) des enfants présentant un syndrome inflammatoire multisystémique (groupe MIS, n = 36). Un test LIPS d’immunoprécipitation a été utilisé afin de mesurer la présence d’anticorps anti-SARS-CoV-2 dirigés contre les domaines C-terminal de la nucléoprotéine N et les domaines S1 et S2 de la protéine de spicule S. Un total de 69 patients séropositifs pour SARS-CoV-2, dont 15 MIS, et de 115 patients séronégatifs (CTL) appariés en fonction de l’âge et du sexe ont été sélectionnés afin de mesurer le niveau d’anticorps dirigés contre le SARS-CoV-2 et les coronavirus saisonniers (HCoV). La séroprévalence et le niveau de réponse anticorps dirigés contre les différents virus ont servi à évaluer si l’infection à SARS-CoV-2 chez les enfants est influencée par une infection antérieure par des coronavirus saisonniers. Principaux résultats: La prévalence des infections à HCoV était très élevée et similaire dans les trois groupes HOS, MIS et CTL. De même, les niveaux de réponse anticorps dirigés contre les HCoV n'étaient pas significativement différents dans les trois groupes et n'étaient pas corrélés au niveau de réponse anticorps anti-SARS-CoV-2 dans les groupes HOS et MIS. Les profils d'anticorps anti-SARS-CoV-2 étaient différents chez les enfants des groupes HOS et MIS, avec un niveau de réponse anti-S1 et –N significativement plus élevés chez les enfants atteints de syndrome inflammatoire multisystémique.Conclusion: Une infection antérieure par les coronavirus saisonniers (évaluée par sérologie) n'interfère pas avec le risque d’être infecté par le SARS-CoV-2 ni avec celui de développer un MIS chez les enfants.
- Published
- 2021
27. Coronaviruses with a SARS-CoV-2-like receptor-binding domain allowing ACE2-mediated entry into human cells isolated from bats of Indochinese peninsula
- Author
-
Temmam, Sarah, primary, Vongphayloth, Khamsing, additional, Salazar, Eduard Baquero, additional, Munier, Sandie, additional, Bonomi, Max, additional, Régnault, Béatrice, additional, Douangboubpha, Bounsavane, additional, Karami, Yasaman, additional, Chretien, Delphine, additional, Sanamxay, Daosavanh, additional, Xayaphet, Vilakhan, additional, Paphaphanh, Phetphoumin, additional, Lacoste, Vincent, additional, Somlor, Somphavanh, additional, Lakeomany, Khaithong, additional, Phommavanh, Nothasin, additional, Pérot, Philippe, additional, Donati, Flora, additional, Bigot, Thomas, additional, Nilges, Michael, additional, Rey, Félix, additional, Werf, Sylvie van der, additional, Brey, Paul, additional, and Eloit, Marc, additional
- Published
- 2021
- Full Text
- View/download PDF
28. Low seroprevalence of COVID-19 in Lao PDR, late 2020
- Author
-
Virachith, Siriphone, primary, Pommelet, Virginie, additional, Calvez, Elodie, additional, Khounvisith, Vilaysone, additional, Sayasone, Somphou, additional, Kounnavong, Sengchanh, additional, Mayxay, Mayfong, additional, Xangsayarath, Phonepadith, additional, Temmam, Sarah, additional, Eloit, Marc, additional, Escriou, Nicolas, additional, Rose, Thierry, additional, Vongphayloth, Khamsing, additional, Hübschen, Judith M., additional, Lacoste, Vincent, additional, Somlor, Somphavanh, additional, Phonekeo, Darouny, additional, Brey, Paul T., additional, and Black, Antony P., additional
- Published
- 2021
- Full Text
- View/download PDF
29. Deep Impact of Random Amplification and Library Construction Methods on Viral Metagenomics Results
- Author
-
Regnault, Béatrice, Bigot, Thomas, Ma, Laurence, Pérot, Philippe, Temmam, Sarah, Eloit, Marc, Découverte de pathogènes – Pathogen discovery, Institut Pasteur [Paris], Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Pôle Biomics (C2RT), Centre de Ressources et de Recherche Technologique - Center for Technological Resources and Research (C2RT), Institut Pasteur [Paris]-Institut Pasteur [Paris], This research was funded by the Thérèse Lebrasseur Prize 2016 awarded by the Fondation de France., Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), and Institut Pasteur [Paris] (IP)-Institut Pasteur [Paris] (IP)
- Subjects
Genomic Library ,MESH: Humans ,[SDV.BIO]Life Sciences [q-bio]/Biotechnology ,lcsh:QR1-502 ,MESH: Genomic Library ,MESH: Limit of Detection ,Genome, Viral ,sensitivity ,Article ,lcsh:Microbiology ,random amplification ,MESH: Nucleic Acid Amplification Techniques ,MESH: Viruses ,Limit of Detection ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Viruses ,Humans ,Metagenomics ,viral metagenomics ,MESH: Genome, Viral ,MESH: Metagenomics ,Nucleic Acid Amplification Techniques - Abstract
International audience; Clinical metagenomics is a broad-range agnostic detection method of pathogens, including novel microorganisms. A major limit is the low pathogen load compared to the high background of host nucleic acids. To overcome this issue, several solutions exist, such as applying a very high depth of sequencing, or performing a relative enrichment of viral genomes associated with capsids. At the end, the quantity of total nucleic acids is often below the concentrations recommended by the manufacturers of library kits, which necessitates to random amplify nucleic acids. Using a pool of 26 viruses representative of viral diversity, we observed a deep impact of the nature of sample (total nucleic acids versus RNA only), the reverse transcription, the random amplification and library construction method on virus recovery. We further optimized the two most promising methods and assessed their performance with fully characterized reference virus stocks. Good genome coverage and limit of detection lower than 100 or 1000 genome copies per mL of plasma, depending on the genome viral type, were obtained from a three million reads dataset. Our study reveals that optimized random amplification is a technique of choice when insufficient amounts of nucleic acid are available for direct libraries constructions.
- Published
- 2021
- Full Text
- View/download PDF
30. Identification d’un nouvel arbovirus neurotrope en France : le virus Umbre
- Author
-
Pérot, Philippe, Bielle, Franck, Bigot, Thomas, Foulongne, Vincent, Bolloré, Karine, Chrétien, Delphine, Gil, Patricia, Gutierrez, Serafin, L'Ambert, Grégory, Mokhtari, Karima, Hellert, Jan, Flamand, Marie, Tamietti, Carole, Coulpier, Muriel, Huard de Verneuil, Anne, Temmam, Sarah, Couderc, Thérèse, De Sousa Cunha, Edouard, Boluda, Susana, Plu, Isabelle, Delisle, Marie Bernadette, Bonneville, Fabrice, Brassat, David, Fieschi, Claire, Malphettes, Marion, Duyckaerts, Charles, Mathon, Bertrand, Demeret, Sophie, Seilhean, Danielle, Eloit, Marc, Pérot, Philippe, Découverte de pathogènes – Pathogen discovery, Institut Pasteur [Paris] (IP), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut du Cerveau = Paris Brain Institute (ICM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Pathogenesis and Control of Chronic and Emerging Infections (PCCEI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Université des Antilles (UA)-Etablissement français du don du sang [Montpellier], Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Entente Interdépartementale pour la démoustication du littoral méditerranéen (EID), Virologie Structurale - Structural Virology, Virologie UMR1161 (VIRO), École nationale vétérinaire - Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Biologie des Infections - Biology of Infection, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service Anatomie et cytologie pathologiques [CHU Toulouse], Pôle Biologie [CHU Toulouse], Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse), Service Neuroradiologie Diagnostique et Thérapeutique [CHU Toulouse], Pôle imagerie médicale [CHU Toulouse], Toulouse Neuro Imaging Center (ToNIC), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Toulouse Mind & Brain Institut (TMBI), Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT), Immunologie clinique [CHU St-Louis], Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre national de référence des Agents Transmissibles Non Conventionnels, Institut de Veille Sanitaire (INVS), École nationale vétérinaire - Alfort (ENVA), Institut Pasteur [Paris], Laboratoire de Neuropathologie Raymond Escourolle, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Pathogénèse et contrôle des infections chroniques (PCCI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Universitaire de Montpellier (CHU Montpellier )-Université de Montpellier (UM), École nationale vétérinaire d'Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Service d'anatomie et cytologie pathologiques [Purpan], Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Hôpital Purpan [Toulouse], CHU Toulouse [Toulouse], Neuroradiologie Diagnostique et Thérapeutique [Toulouse], Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital Purpan [Toulouse], CHU Toulouse [Toulouse]-CHU Toulouse [Toulouse], Service de Neurochirurgie [CHU Pitié-Salpêtrière], and École nationale vétérinaire d'Alfort (ENVA)
- Subjects
[SDV.EE]Life Sciences [q-bio]/Ecology, environment ,métagénomique ,[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,moustiques ,[SDV.MHEP.AHA] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] ,encéphalites ,orthobunyavirus ,[SDV.EE] Life Sciences [q-bio]/Ecology, environment ,arbovirus ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,NGS ,Umbre virus ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,[SDV.MHEP.AHA]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] ,virologie ,[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] - Abstract
International audience; Notre laboratoire a mis en place une collaboration avec le laboratoire de neuropathologie de l’hôpital de la Pitié-Salpêtrière pour explorer une série post-mortem de cas d’encéphalites humaines d’étiologie inconnue par un séquençage NGS profond et non ciblé du transcriptome, à la recherche de séquences de pathogènes. Le virus Umbre, un virus du genre orthobunyavirus, a été identifié chez deux patients immunodéprimés décédés en 2013 et 2019. Le virus infecte des neurones du cortex cérébral, la moelle épinière et le foie. Le virus Umbre a été décrit initialement dans les années 1950 dans des populations de moustiques Culex en zone Asie Pacifique, mais n’avait auparavant ni été isolé chez des mammifères (dont l’homme), ni détecté en Europe. Cette infection par le virus Umbre chez deux patients français, dont l’un vivait dans la région Occitanie et n’avait jamais voyagé en dehors de France métropolitaine, et l’autre avait fait une croisière en méditerranée avant l’apparition des symptômes, nous a amené à rechercher l’origine de la contamination dans des moustiques. Une investigation réalisée en partenariat avec le CIRAD de Montpellier et l’EID Méditerranée a permis d’identifier des séquences du genre orthobunyavirus très proches du virus Umbre dans des moustiques Culex de Camargue. Une enquête sérologique par un test développé à l’Institut Pasteur sur une population du sud de la France composée d’environ 300 personnes « contrôles » et d’une vingtaine de cas « encéphalites » n’a pas mis en évidence de réponse anticorps contre le virus Umbre. Ce résultat suggère que la fréquence de l’infection dans la population générale est au plus faible, ce qui ne reflète donc pas le risque pour les personnes immunodéprimées. Cette découverte d’un nouvel arbovirus responsable d’encéphalite, et son existence en France, présente un intérêt particulier en matière de santé publique.
- Published
- 2021
31. Influenza A(H1N1) pdm09 virus in pigs, Reunion Island
- Author
-
Cardinale, Eric, Pascalis, Herve, Temmam, Sarah, Herve, Severine, Saulnier, Aure, Turpin, Magali, Barbier, Nicolas, Hoarau, Johny, Queguiner, Stephane, Gorin, Stephane, Foray, Coralie, Roger, Matthieu, Porphyre, Vincent, Andre, Paul, Thomas, Thierry, de Lamballerie, Xavier, Dellagi, Koussay, and Simon, Gaelle
- Subjects
Swine influenza -- Risk factors -- Research -- Diagnosis ,Influenza viruses -- Health aspects -- Genetic aspects -- Research ,Genetic variation -- Research ,Health - Abstract
During 2009, pandemic influenza A(H1N1)pdm09 virus affected humans on Reunion Island. Since then, the virus has sustained circulation among local swine herds, raising concerns about the potential for genetic evolution [...]
- Published
- 2012
- Full Text
- View/download PDF
32. Monitoring Silent Spillovers Before Emergence: A Pilot Study at the Tick/Human Interface in Thailand
- Author
-
Temmam, Sarah, Chrétien, Delphine, Bigot, Thomas, Dufour, Evelyne, Petres, Stéphane, Desquesnes, Marc, Devillers, Elodie, Dumarest, Marine, Yousfi, Léna, Jittapalapong, Sathaporn, Karnchanabanthoeng, Anamika, Chaisiri, Kittipong, Gagnieur, Léa, Cosson, Jean-Francois, Vayssier-Taussat, Muriel, Morand, Serge, Moutailler, Sara, Eloit, Marc, Biologie des Infections - Biology of Infection, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Découverte de Pathogènes - Pathogen Discovery, Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Centre de Ressources et de Recherche Technologique - Center for Technological Resources and Research (C2RT), Institut Pasteur [Paris], Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Interactions hôtes-vecteurs-parasites-environnement dans les maladies tropicales négligées dues aux trypanosomatides (UMR INTERTRYP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université de Bordeaux (UB), Kasetsart University (KU), Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Mahidol University [Bangkok], Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), This work was supported by Laboratoire d'Excellence Integrative Biology of Emerging Infectious Diseases (grant no. ANR-10-LABX-62-IBEID), by the Direction Internationale de l'Institut Pasteur, and by the ANR projects BiodivHealthSEA and FutureHealthSEA (ANR-17-CE35-0003-02) for fieldwork studies in Thailand., The authors want to thank Jacques Bellalou and his team of the Production and Purification of Recombinant Proteins Technological Platform from the Institut Pasteur for their help in expression of recombinant proteins, Yves Jacob and Mélanie Dos Santos for their technical assistance with the luminometer, the ICAReB Platform from the Institut Pasteur for giving access to human control sera, Yves Gaudin for his analysis of Thailand tick rhabdovirus genome structure, Nicole Pavio for providing HEV positive and negative serum controls used to validate LIPS assay, and all the people involved in sample collection in Thailand., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-11-CEPL-0002,BiodivHealthSEA,Impacts et perceptions locales des changements globaux : santé, biodiversité et zoonoses en Asie du Sud-Est(2011), ANR-17-CE35-0003,FutureHealthSEA,Scénarios de la santé en Asie du Sud-Est: changements d'utilisation des terres, changement climatique et maladies infectieuses(2017), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École Pratique des Hautes Études (EPHE), TEMMAM, Sarah, Integrative Biology of Emerging Infectious Diseases - - IBEID2010 - ANR-10-LABX-0062 - LABX - VALID, Changements Environnementaux Planétaires et Sociétés - Impacts et perceptions locales des changements globaux : santé, biodiversité et zoonoses en Asie du Sud-Est - - BiodivHealthSEA2011 - ANR-11-CEPL-0002 - CEP&S - VALID, and Scénarios de la santé en Asie du Sud-Est: changements d'utilisation des terres, changement climatique et maladies infectieuses - - FutureHealthSEA2017 - ANR-17-CE35-0003 - AAPG2017 - VALID
- Subjects
[SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,virome ,spillover ,viruses ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,parasitic diseases ,emergence ,LIPS ,Microbiology ,tick ,Original Research - Abstract
International audience; Emerging zoonoses caused by previously unknown agents are one of the most important challenges for human health because of their inherent inability to be predictable, conversely to emergences caused by previously known agents that could be targeted by routine surveillance programs. Emerging zoonotic infections either originate from increasing contacts between wildlife and human populations, or from the geographical expansion of hematophagous arthropods that act as vectors, this latter being more capable to impact large-scale human populations. While characterizing the viral communities from candidate vectors in high-risk geographical areas is a necessary initial step, the need to identify which viruses are able to spill over and those restricted to their hosts has recently emerged. We hypothesized that currently unknown tick-borne arboviruses could silently circulate in specific biotopes where mammals are highly exposed to tick bites, and implemented a strategy that combined high-throughput sequencing with broad-range serological techniques to both identify novel arboviruses and tick-specific viruses in a ticks/mammals interface in Thailand. The virome of Thai ticks belonging to the Rhipicephalus, Amblyomma, Dermacentor, Hyalomma, and Haemaphysalis genera identified numerous viruses, among which several viruses could be candidates for future emergence as regards to their phylogenetic relatedness with known tick-borne arboviruses. Luciferase immunoprecipitation system targeting external viral proteins of viruses identified among the Orthomyxoviridae, Phenuiviridae, Flaviviridae, Rhabdoviridae, and Chuviridae families was used to screen human and cattle Thai populations highly exposed to tick bites. Although no positive serum was detected for any of the six viruses selected, suggesting that these viruses are not infecting these vertebrates, or at very low prevalence (upper estimate 0.017% and 0.047% in humans and cattle, respectively), the virome of Thai ticks presents an extremely rich viral diversity, among which novel tick-borne arboviruses are probably hidden and could pose a public health concern if they emerge. The strategy developed in this pilot study, starting from the inventory of viral communities of hematophagous arthropods to end by the identification of viruses able (or likely unable) to infect vertebrates, is the first step in the prediction of putative new emergences and could easily be transposed to other reservoirs/vectors/susceptible hosts interfaces.
- Published
- 2019
- Full Text
- View/download PDF
33. The virome ofRhipicephalus,DermacentorandHaemaphysalisticks from Eastern Romania includes novel viruses with potential relevance for public health
- Author
-
Bratuleanu, Bianca Elena, primary, Temmam, Sarah, additional, Chrétien, Delphine, additional, Regnault, Béatrice, additional, Pérot, Philippe, additional, Bouchier, Christiane, additional, Bigot, Thomas, additional, Savuța, Gheorghe, additional, and Eloit, Marc, additional
- Published
- 2021
- Full Text
- View/download PDF
34. SARS-CoV-2 infection in schools in a northern French city: a retrospective serological cohort study in an area of high transmission, France, January to April 2020
- Author
-
Fontanet, Arnaud, primary, Tondeur, Laura, additional, Grant, Rebecca, additional, Temmam, Sarah, additional, Madec, Yoann, additional, Bigot, Thomas, additional, Grzelak, Ludivine, additional, Cailleau, Isabelle, additional, Besombes, Camille, additional, Ungeheuer, Marie-Noëlle, additional, Renaudat, Charlotte, additional, Perlaza, Blanca Liliana, additional, Arowas, Laurence, additional, Jolly, Nathalie, additional, Pellerin, Sandrine Fernandes, additional, Kuhmel, Lucie, additional, Staropoli, Isabelle, additional, Huon, Christèle, additional, Chen, Kuang-Yu, additional, Crescenzo-Chaigne, Bernadette, additional, Munier, Sandie, additional, Charneau, Pierre, additional, Demeret, Caroline, additional, Bruel, Timothée, additional, Eloit, Marc, additional, Schwartz, Olivier, additional, and Hoen, Bruno, additional
- Published
- 2021
- Full Text
- View/download PDF
35. Prior infection by seasonal coronaviruses, as assessed by serology, does not prevent SARS-CoV-2 infection and disease in children, France, April to June 2020
- Author
-
Sermet-Gaudelus, Isabelle, primary, Temmam, Sarah, additional, Huon, Christèle, additional, Behillil, Sylvie, additional, Gajdos, Vincent, additional, Bigot, Thomas, additional, Lurier, Thibaut, additional, Chrétien, Delphine, additional, Backovic, Marija, additional, Delaunay-Moisan, Agnès, additional, Donati, Flora, additional, Albert, Mélanie, additional, Foucaud, Elsa, additional, Mesplées, Bettina, additional, Benoist, Grégoire, additional, Faye, Albert, additional, Duval-Arnould, Marc, additional, Cretolle, Célia, additional, Charbit, Marina, additional, Aubart, Mélodie, additional, Auriau, Johanne, additional, Lorrot, Mathie, additional, Kariyawasam, Dulanjalee, additional, Fertitta, Laura, additional, Orliaguet, Gilles, additional, Pigneur, Bénédicte, additional, Bader-Meunier, Brigitte, additional, Briand, Coralie, additional, Enouf, Vincent, additional, Toubiana, Julie, additional, Guilleminot, Tiffany, additional, van der Werf, Sylvie, additional, Leruez-Ville, Marianne, additional, and Eloit, Marc, additional
- Published
- 2021
- Full Text
- View/download PDF
36. RVDB-prot, a reference viral protein database and its HMM profiles [version 2; peer review: 2 approved]
- Author
-
Bigot, Thomas, Temmam, Sarah, Pérot, Philippe, Eloit, Marc, Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Découverte de pathogènes – Pathogen discovery, Institut Pasteur [Paris], École nationale vétérinaire d'Alfort (ENVA), We would like to thank Peter Skewes-Cox, Jr., author of vFAM database for kindly providing his scripts which were an inspiration for the earlier versions of RVDB-prot. We thank Natalia Pietrosemoli for her help in the editing of the manuscript. This work used the computational and storage services (TARS cluster) provided by the IT department at Institut Pasteur, Paris., Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP), and École nationale vétérinaire - Alfort (ENVA)
- Subjects
annotations ,natural sciences ,virus ,hmm ,clusters ,[INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM] ,genomes ,[SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM] ,proteins ,database - Abstract
International audience; We present RVDB-prot, a database corresponding to the protein equivalent of the nucleic acid reference virus database RVDB. Protein databases can be helpful to perform more sensitive protein sequence comparisons. Similarly to its homologous public repository, RVDB-prot aims to provide reliable and accurately annotated unique entries, while including also an Hidden Markov Model (HMM) protein profiles database for distant protein searching.
- Published
- 2020
- Full Text
- View/download PDF
37. Cocirculation of 2 genotypes of Toscana virus, Southeastern France
- Author
-
Charrel, Remi N., Izri, Arezki, Temmam, Sarah, Delaunay, Pascal, Toga, Isabelle, Dumon, Henri, Marty, Pierre, de Lamballerie, Xavier, and Parola, Philippe
- Subjects
Genotype -- Genetic aspects ,Disease transmission -- Genetic aspects - Abstract
Toscana virus (TOSV), an arthropodborne phlebovirus transmitted by sandflies, can cause febrile illness and meningitis. The vector of TOSV in France was unknown, We detected TOSV RNA in 2 (female [...]
- Published
- 2007
38. RNA viruses of Amblyomma variegatum and Rhipicephalus microplus and cattle susceptibility in the French Antilles
- Author
-
Gondard, Mathilde, Temmam, Sarah, Devillers, Elodie, Pinarello, Valérie, Bigot, Thomas, Chrétien, Delphine, Aprelon, Rosalie, Vayssier-Taussat, Muriel, Albina, Emmanuel, Eloit, Marc, Moutailler, Sara, Gondard, Mathilde, Temmam, Sarah, Devillers, Elodie, Pinarello, Valérie, Bigot, Thomas, Chrétien, Delphine, Aprelon, Rosalie, Vayssier-Taussat, Muriel, Albina, Emmanuel, Eloit, Marc, and Moutailler, Sara
- Abstract
Ticks transmit a wide variety of pathogens including bacteria, parasites and viruses. Over the last decade, numerous novel viruses have been described in arthropods, including ticks, and their characterization has provided new insights into RNA virus diversity and evolution. However, little is known about their ability to infect vertebrates. As very few studies have described the diversity of viruses present in ticks from the Caribbean, we implemented an RNA-sequencing approach on Amblyomma variegatum and Rhipicephalus microplus ticks collected from cattle in Guadeloupe and Martinique. Among the viral communities infecting Caribbean ticks, we selected four viruses belonging to the Chuviridae, Phenuiviridae and Flaviviridae families for further characterization and designing antibody screening tests. While viral prevalence in individual tick samples revealed high infection rates, suggesting a high level of exposure of Caribbean cattle to these viruses, no seropositive animals were detected. These results suggest that the Chuviridae- and Phenuiviridae-related viruses identified in the present study are more likely tick endosymbionts, raising the question of the epidemiological significance of their occurrence in ticks, especially regarding their possible impact on tick biology and vector capacity. The characterization of these viruses might open the door to new ways of preventing and controlling tick-borne diseases.
- Published
- 2020
39. Whole genome sequencing and phylogenetic characterization of a novel bat-associated picornavirus-like virus with an unusual genome organization
- Author
-
Temmam, Sarah, Hul, Vibol, Bigot, Thomas, Bessaud, Maël, Chrétien, Delphine, Hoem, Thavry, Gorman, Christopher, Duong, Veasna, Dussart, Philippe, Cappelle, Julien, Eloit, Marc, Temmam, Sarah, Hul, Vibol, Bigot, Thomas, Bessaud, Maël, Chrétien, Delphine, Hoem, Thavry, Gorman, Christopher, Duong, Veasna, Dussart, Philippe, Cappelle, Julien, and Eloit, Marc
- Abstract
The order Picornavirales is one of the most important viral orders in terms of virus diversity and genome organizations, ranging from a mono- or bi-cistronic expression strategies to the recently described poly-cistronic Polycipiviridae viruses. We report here the description and characterization of a novel picorna-like virus identified in rectal swabs of frugivorous bats in Cambodia that presents an unusual genome organization. Kandabadicivirus presents a unique genome architecture and distant phylogenetic relationship to the proposed Badiciviridae family. These findings highlight a high mosaicism of genome organizations among the Picornavirales.
- Published
- 2020
40. THE VIROME OF RHIPICEPHALUS, DERMACENTOR AND HAEMAPHYSALIS TICKS FROM EASTERN ROMANIA INCLUDES NOVEL VIRUSES WITH POTENTIAL RELEVANCE FOR PUBLIC HEALTH
- Author
-
Brătuleanu, Bianca, primary, Temmam, Sarah, additional, Chretien, Delphine, additional, Regnault, Béatrice, additional, Perot, Philippe, additional, Bouchier, Christiane, additional, Bigot, Thomas, additional, Savuta, Gheorghe, additional, and Eloit, Marc, additional
- Published
- 2021
- Full Text
- View/download PDF
41. The virome of Rhipicephalus, Dermacentor and Haemaphysalis ticks from Eastern Romania includes novel viruses with potential relevance for public health.
- Author
-
Bratuleanu, Bianca Elena, Temmam, Sarah, Chrétien, Delphine, Regnault, Béatrice, Pérot, Philippe, Bouchier, Christiane, Bigot, Thomas, Savuţa, Gheorghe, and Eloit, Marc
- Subjects
- *
TICKS , *DERMACENTOR , *RHIPICEPHALUS , *TICK-borne diseases , *BIRD migration , *VIRUS diversity - Abstract
Ticks are involved in the transmission of various pathogens and several tick-borne diseases cause significant problems for the health of humans and livestock. The composition of viral communities in ticks and their interactions with pathogens, is poorly understood, particularly in Eastern Europe, an area that represents a major hub for animal-arthropod vectors exchanges (e.g., via bird migrations). The aim of this study was to describe the virome of Dermacentor sp., Rhipicephalus sp. and Haemaphysalis sp. ticks collected from relatively little studied regions of Romania (Iasi and Tulcea counties) located at the intersection of various biotopes, countries and routes of migrations. We also focused the study on viruses that could potentially have relevance for human and animal health. In 2019, more than 500 ticks were collected from the vegetation and from small ruminants and analysed by high-throughput transcriptome sequencing. Among the viral communities infecting Romanian ticks, viruses belonging to the Flaviviridae, Phenuiviridae and Nairoviridae families were identified and full genomes were derived. Phylogenetic analyses placed them in clades where mammalian isolates are found, suggesting that these viruses could constitute novel arboviruses. The characterization of these communities increase the knowledge of the diversity of viruses in Eastern Europe and provides a basis for further studies about the interrelationship between ticks and tick-borne viruses. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
42. Absence of SARS-CoV-2 infection in cats and dogs in close contact with a cluster of COVID-19 patients in a veterinary campus
- Author
-
Temmam, Sarah, primary, Barbarino, Alix, additional, Maso, Djérène, additional, Behillil, Sylvie, additional, Enouf, Vincent, additional, Huon, Christèle, additional, Jaraud, Ambre, additional, Chevallier, Lucie, additional, Backovic, Marija, additional, Pérot, Philippe, additional, Verwaerde, Patrick, additional, Tiret, Laurent, additional, van der Werf, Sylvie, additional, and Eloit, Marc, additional
- Published
- 2020
- Full Text
- View/download PDF
43. A comparison of four serological assays for detecting anti–SARS-CoV-2 antibodies in human serum samples from different populations
- Author
-
Grzelak, Ludivine, primary, Temmam, Sarah, additional, Planchais, Cyril, additional, Demeret, Caroline, additional, Tondeur, Laura, additional, Huon, Christèle, additional, Guivel-Benhassine, Florence, additional, Staropoli, Isabelle, additional, Chazal, Maxime, additional, Dufloo, Jeremy, additional, Planas, Delphine, additional, Buchrieser, Julian, additional, Rajah, Maaran Michael, additional, Robinot, Remy, additional, Porrot, Françoise, additional, Albert, Mélanie, additional, Chen, Kuang-Yu, additional, Crescenzo-Chaigne, Bernadette, additional, Donati, Flora, additional, Anna, François, additional, Souque, Philippe, additional, Gransagne, Marion, additional, Bellalou, Jacques, additional, Nowakowski, Mireille, additional, Backovic, Marija, additional, Bouadma, Lila, additional, Le Fevre, Lucie, additional, Le Hingrat, Quentin, additional, Descamps, Diane, additional, Pourbaix, Annabelle, additional, Laouénan, Cédric, additional, Ghosn, Jade, additional, Yazdanpanah, Yazdan, additional, Besombes, Camille, additional, Jolly, Nathalie, additional, Pellerin-Fernandes, Sandrine, additional, Cheny, Olivia, additional, Ungeheuer, Marie-Noëlle, additional, Mellon, Guillaume, additional, Morel, Pascal, additional, Rolland, Simon, additional, Rey, Felix A., additional, Behillil, Sylvie, additional, Enouf, Vincent, additional, Lemaitre, Audrey, additional, Créach, Marie-Aude, additional, Petres, Stephane, additional, Escriou, Nicolas, additional, Charneau, Pierre, additional, Fontanet, Arnaud, additional, Hoen, Bruno, additional, Bruel, Timothée, additional, Eloit, Marc, additional, Mouquet, Hugo, additional, Schwartz, Olivier, additional, and van der Werf, Sylvie, additional
- Published
- 2020
- Full Text
- View/download PDF
44. Prior infection by seasonal coronaviruses does not prevent SARS-CoV-2 infection and associated Multisystem Inflammatory Syndrome in children
- Author
-
Sermet-Gaudelus, Isabelle, primary, Temmam, Sarah, additional, Huon, Christèle, additional, Behillil, Sylvie, additional, Gajdos, Vincent, additional, Bigot, Thomas, additional, Lurier, Thibaut, additional, Chrétien, Delphine, additional, Backovic, Marija, additional, Moisan-Delaunay, Agnès, additional, Donati, Flora, additional, Albert, Mélanie, additional, Foucaud, Elsa, additional, Mesplées, Bettina, additional, Benoist, Grégoire, additional, Faye, Albert, additional, Duval-Arnould, Marc, additional, Cretolle, Célia, additional, Charbit, Marina, additional, Aubart, Mélodie, additional, Auriau, Johanne, additional, Lorrot, Mathie, additional, Kariyawasam, Dulanjalee, additional, Fertitta, Laura, additional, Orliaguet, Gilles, additional, Pigneur, Bénédicte, additional, Bader-Meunier, Brigitte, additional, Briand, Coralie, additional, Enouf, Vincent, additional, Toubiana, Julie, additional, Guilleminot, Tiffany, additional, van der Werf, Sylvie, additional, Leruez-Ville, Marianne, additional, and Eloit, Marc, additional
- Published
- 2020
- Full Text
- View/download PDF
45. Identification of Umbre Orthobunyavirus as a Novel Zoonotic Virus Responsible for Lethal Encephalitis in 2 French Patients with Hypogammaglobulinemia
- Author
-
Pérot, Philippe, primary, Bielle, Franck, additional, Bigot, Thomas, additional, Foulongne, Vincent, additional, Bolloré, Karine, additional, Chrétien, Delphine, additional, Gil, Patricia, additional, Gutiérrez, Serafín, additional, L’Ambert, Grégory, additional, Mokhtari, Karima, additional, Hellert, Jan, additional, Flamand, Marie, additional, Tamietti, Carole, additional, Coulpier, Muriel, additional, Huard de Verneuil, Anne, additional, Temmam, Sarah, additional, Couderc, Thérèse, additional, De Sousa Cunha, Edouard, additional, Boluda, Susana, additional, Plu, Isabelle, additional, Delisle, Marie Bernadette, additional, Bonneville, Fabrice, additional, Brassat, David, additional, Fieschi, Claire, additional, Malphettes, Marion, additional, Duyckaerts, Charles, additional, Mathon, Bertrand, additional, Demeret, Sophie, additional, Seilhean, Danielle, additional, and Eloit, Marc, additional
- Published
- 2020
- Full Text
- View/download PDF
46. SURVEY OF CRIMEAN-CONGO HEMORRHAGIC FEVER VIRUS IN RHIPICEPHALUS AND DERMACENTOR SPECIES TICKS FROM SOUTH-EASTERN ROMANIA
- Author
-
tuleanu, Bianca Br, primary, Chretien, Delphine, additional, Regnault, B atrice, additional, Temmam, Sarah, additional, Perot, Philippe, additional, Anita, Adriana, additional, Anita, Dragos, additional, Savuta, Gheorghe, additional, and Eloit, Marc, additional
- Published
- 2020
- Full Text
- View/download PDF
47. SARS-CoV-2 serological analysis of COVID-19 hospitalized patients, pauci-symptomatic individuals and blood donors
- Author
-
Grzelak, Ludivine, primary, Temmam, Sarah, additional, Planchais, Cyril, additional, Demeret, Caroline, additional, Huon, Christèle, additional, Guivel-Benhassine, Florence, additional, Staropoli, Isabelle, additional, Chazal, Maxime, additional, Dufloo, Jeremy, additional, Planas, Delphine, additional, Buchrieser, Julian, additional, Rajah, Maaran Michael, additional, Robinot, Remy, additional, Porrot, Françoise, additional, Albert, Mélanie, additional, Chen, Kuang-Yu, additional, Crescenzo, Bernadette, additional, Donati, Flora, additional, Anna, François, additional, Souque, Philippe, additional, Gransagne, Marion, additional, Bellalou, Jacques, additional, Nowakowski, Mireille, additional, Backovic, Marija, additional, Bouadma, Lila, additional, Fevre, Lucie Le, additional, Hingrat, Quentin Le, additional, Descamps, Diane, additional, Pourbaix, Annabelle, additional, Yazdanpanah, Yazdan, additional, Tondeur, Laura, additional, Besombes, Camille, additional, Ungeheuer, Marie-Noëlle, additional, Mellon, Guillaume, additional, Morel, Pascal, additional, Rolland, Simon, additional, Rey, Felix, additional, Behillil, Sylvie, additional, Enouf, Vincent, additional, Lemaitre, Audrey, additional, Créach, Marie-Aude, additional, Petres, Stephane, additional, Escriou, Nicolas, additional, Charneau, Pierre, additional, Fontanet, Arnaud, additional, Hoen, Bruno, additional, Bruel, Timothée, additional, Eloit, Marc, additional, Mouquet, Hugo, additional, Schwartz, Olivier, additional, and van der Werf, Sylvie, additional
- Published
- 2020
- Full Text
- View/download PDF
48. Cluster of COVID-19 in northern France: A retrospective closed cohort study
- Author
-
Fontanet, Arnaud, primary, Tondeur, Laura, additional, Madec, Yoann, additional, Grant, Rebecca, additional, Besombes, Camille, additional, Jolly, Nathalie, additional, Pellerin, Sandrine Fernandes, additional, Ungeheuer, Marie-Noëlle, additional, Cailleau, Isabelle, additional, Kuhmel, Lucie, additional, Temmam, Sarah, additional, Huon, Christèle, additional, Chen, Kuang-Yu, additional, Crescenzo, Bernadette, additional, Munier, Sandie, additional, Demeret, Caroline, additional, Grzelak, Ludivine, additional, Staropoli, Isabelle, additional, Bruel, Timothée, additional, Gallian, Pierre, additional, Cauchemez, Simon, additional, van der Werf, Sylvie, additional, Schwartz, Olivier, additional, Eloit, Marc, additional, and Hoen, Bruno, additional
- Published
- 2020
- Full Text
- View/download PDF
49. Whole genome sequencing and phylogenetic characterization of a novel bat-associated picornavirus-like virus with an unusual genome organization
- Author
-
Temmam, Sarah, primary, Hul, Vibol, additional, Bigot, Thomas, additional, Bessaud, Maël, additional, Chrétien, Delphine, additional, Hoem, Thavry, additional, Gorman, Christopher, additional, Duong, Veasna, additional, Dussart, Philippe, additional, Cappelle, Julien, additional, and Eloit, Marc, additional
- Published
- 2020
- Full Text
- View/download PDF
50. Viromics on Honey-Baited FTA Cards as a New Tool for the Detection of Circulating Viruses in Mosquitoes
- Author
-
Birnberg, Lotty, primary, Temmam, Sarah, additional, Aranda, Carles, additional, Correa-Fiz, Florencia, additional, Talavera, Sandra, additional, Bigot, Thomas, additional, Eloit, Marc, additional, and Busquets, Núria, additional
- Published
- 2020
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.