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Purpose The aim of this study was to test the hypothesis that subjective thermal strain can reduce endurance performance independently from the general physiological strain normally associated with impaired endurance performance in the heat. Methods In 20 °C and 44% relative humidity, 12 endurance-trained athletes (1♀ 11♂; mean ± SD; age: 27 ± 6 y; VO2max: 61 ± 6 ml/kg/min) performed a time to exhaustion (TTE) test in two different experimental conditions: with an electric heat pad applied to the subjects' upper back (HP) and control (CON: without heat pad). In both conditions, subjects cycled to volitional exhaustion at 70% of their VO2max. Cardiorespiratory, metabolic, thermoregulatory and perceptual responses were measured throughout the TTE test and compared at 0%, 50% and 100% isotime and at exhaustion. Results TTE was reduced by 9% in HP (2092 ± 305 s) compared to CON (2292 ± 344 s; p = .023). The main effect of condition on thermal discomfort at isotime (p = .002), the effect of condition on thermal sensation at 0% isotime (p = .004) and the condition by isotime interaction on rating of perceived exertion (p = .036) indicated higher subjective thermal strain in HP compared to CON. None of the measured cardiorespiratory, metabolic and thermoregulatory variables differed significantly between conditions. Conclusion Our novel experimental manipulation (HP) was able to induce significant subjective thermal strain and reduce endurance performance in a temperate environment without inducing the general physiological strain normally associated with impaired endurance performance in the heat. These results suggest that subjective thermal strain is an important and independent mediator of the heat-induced impairment in endurance performance.

Objectives To determine if intermittent exercise-heat exposures (IHE) every fifth day sustain heat acclimation (HA) adaptations 25 days after initial HA. Design Randomized control trial. Methods Sixteen non-heat acclimatized men heat acclimated during 10–11 days of exercise in the heat (40 °C, 40% RH). A heat stress test (120 min, 45% V ˙ O2peak) before (Pre HA) and after HA (Post HA) in similar hot conditions assessed HA status. Pair-matched participants were randomized into a control group (CON; n = 7) that exercised in a temperate environment (24 °C, 21%RH) or IHE group (n = 9) that exercised in a hot environment (40 °C, 40%RH) every fifth day for 25 days following HA (+25d) with out-of-laboratory exercise intensity and duration recorded. Both groups completed +25d in the hot condition. Results Both groups heat acclimated similarly (p > 0.05) evidenced by lower heart rate (HR), thermoregulatory, physiological, and perceptual responses (perceived exertion, fatigue, thermal sensation) Pre HA vs. Post HA (p ≤ 0.05). At +25d, post-exercise HR (p = 0.01) and physiological strain index (p Conclusions Exercise-heat exposures every fifth day for 25 days and regular intense physical activity after HA sustained HR and Tre adaptations and reduced perceptual and physiological strain during exercise-heat stress ∼1 month later.

Contemporary practice favors refilling sublingual nitroglycerin (SL NTG) every 3 to 6 months. This recommendation is based on antiquated data that does not consider the reformulated tablet and the improved manufacturing process. Our objective was to investigate the stability of SL NTG over time using simulated real-life scenarios in comparison to controlled storage conditions. This was an open-label study of 100- and 25-count commercial SL NTG bottles stored in either controlled temperature and relative humidity conditions, or carried in a pocket or purse. SL NTG potency (chemical stability) was assessed using high performance liquid chromatography and physical stability was assessed by changes in tablet weights over time through the labeled expiration date. Both chemical and physical stability of SL NTG were affected by environmental and physical factors. High temperature storage resulted in the most rapid loss of potency. Tablets carried in a pant pocket lost potency faster than those carried in a purse. Potency was also dependent on headspace of the bottle. Tablets stored in the original bottle in a temperate environment could be expected to maintained potency for more than 2 years when carried in a purse, irrespective of package size. When carried in a pant pocket, potency of a 25-count bottle was maintained for 2 years, whereas potency of a 100-count bottle fell below acceptable limits at 12 months. In conclusion, since potency is dependent on temperature, headspace, and carrying practices, frequency of SL NTG refills should be based on individual patient behavior.

Wildlife managers design artificial structures, such as bird houses and bat boxes, to provide alternative nesting and roosting sites that aid wildlife conservation. However, artificial structures for wildlife may not be equally efficient at all sites due to varying climate or habitat characteristics influencing thermal properties. For example, bat boxes are a popular measure employed to provide compensatory or supplementary roost sites for bats and educate the public. Yet, bat boxes are often thermally unstable or too cold to fulfill reproductive females needs in northern temperate environments. To help improve the thermodynamics of bat boxes, we tested the effect of (1) three mountings, (2) four orientations, and (3) twelve bat box designs on the internal temperature of bat boxes. We recorded temperatures in bat boxes across a climate gradient at seven sites in Quebec, Canada. Bat boxes mounted on buildings had warmer microclimates at night than those on poles and those facing east warmed sooner in the morning than those facing west or south. Our best new model based on passive solar architecture (Ncube PH1) increased the time in the optimal temperature range (22–40 °C) of targeted species by up to 13% compared to the most commonly used model (Classic 4-chamber) when mounted on a building with an east orientation (other designs presented in the Supplementary Information). Based on bioenergetic models, we estimated that bats saved up to 8% of their daily energy using the Ncube PH1 compared to the Classic 4-chamber when mounted on a building with an east orientation. We demonstrate that the use of energy-saving concepts from architecture can improve the thermal performance of bat boxes and potentially other wildlife structures as well.

Implementing permissive dehydration (DEH) during short-term heat acclimation (HA) may accelerate adaptations to the heat. However, HA with DEH may augment risk for acute kidney injury (AKI). This study investigated the effect of HA with permissive DEH on time-trial performance and markers of AKI. Fourteen moderately trained men (age and VO2max = 25 ± 0.5 yr and 51.6 ± 1.8 mL.kg−1.min−1) were randomly assigned to DEH or euhydration (EUH). Time-trial performance and VO2max were assessed in a temperate environment before and after 7 d of HA. Heat acclimation consisted of 90 min of cycling in an environmental chamber (40 °C, 35% RH). Neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule-1 (KIM-1) were assessed pre- and post-exercise on day 1 and day 7 of HA. Following HA, VO2max did not change in either group (p = 0.099), however, time-trial performance significantly improved (3%, p &lt, 0.01) with no difference between groups (p = 0.485). Compared to pre-exercise, NGAL was not significantly different following day 1 and 7 of HA (p = 0.113) with no difference between groups (p = 0.667). There was a significant increase in KIM-1 following day 1 and 7 of HA (p = 0.002) with no difference between groups (p = 0.307). Heat acclimation paired with permissive DEH does not amplify improvements in VO2max or time-trial performance in a temperate environment versus EUH and does not increase markers of AKI.

We investigated the capability of transfluthrin on US military camouflage netting to reduce collections of tabanid biting flies in a warm-temperate field environment on the Gulf Coast of Florida. We found that transfluthrin significantly reduced collections of a variety of medically and veterinarily important tabanids inside protected areas by up to 96% upon initial treatment and up to 74% after 20 days posttreatment. These results suggest that transfluthrin could be an effective element in the US Department of Defense integrated pest management system and leveraged in civilian scenarios to protect livestock and humans from potential mechanical transmission of pathogens and disruption of activities caused by painful bites.

We investigated whether an 11-day heat acclimation programme (HA) enhanced endurance performance in a temperate environment, and the mechanisms underpinning any ergogenic effect. Twenty-four males (V̇O2max: 56.7±7.5 mL∙kg-1∙min-1) completed either: i) HA consisting of 11 consecutive daily exercise sessions (60-90 minutes·day-1; n=16) in a hot environment (40°C, 50% RH) or; ii) duration and exertion matched exercise in cool conditions (CON; n=8 [11°C, 60% RH]). Before and after each programme power at lactate threshold, mechanical efficiency, VO2max, peak power output (PPO) and work done during a 30-minute cycle trial (T30) were determined under temperate conditions (22°C, 50% RH). HA reduced resting (-0.34±0.30°C) and exercising (-0.43±0.30°C) rectal temperature, and increased whole-body sweating (+0.37±0.31 L·hr-1) (all P≤0.001), with no change in CON. Plasma volume increased in HA (10.1±7.2%, P

BACKGROUND The effects of a reduced or mildly elevated exercising muscle temperature on the graded exercise test (GXT) performance have yet to be studied. The present study clarified the effects of a range of exercising muscle temperatures on GXT performance in a temperate environment. METHODS Eight male subjects (age: 24.0±0.5 years old; height: 175±2 cm; weight: 64.8±2.0 kg; peak oxygen consumption [VO 2peak ]: 51.1±2.4 mL/kg/min) performed 4 GXTs at different exercising muscle temperatures using a cycle-ergometer in a temperate environment (24.1±0.2 °C). The exercise began at 0.3 kilopond (kp) with 60 revolutions per minute (rpm) and increased 0.3 kp every minute until volitional exhaustion. Subjects passively cooled (averaged deep thigh and calf temperature [Tmm], cold: 31 °C or cool: 33 °C) or warmed (Tmm; warm: 35 °C or hot: 37 °C) the exercising muscle using water perfusion pants throughout the test. The peak oxygen consumption (VO 2peak ), exercise time to exhaustion (TTE), heart rate (HR), tympanic (Tty) and mean body temperature (Tb), and total sweat loss were also measured. RESULTS No significant differences were observed in the VO 2peak or TTE among the 4 conditions; however, the HR, Tb, and total sweat loss were significantly higher (P

Castell Henllys, a partially reconstructed Iron Age settlement in south-west Wales, has been the site of long-term experiments in building reconstruction for 35 years. The first roundhouse to be excavated and then reconstructed on the site was due to be replaced in 2017, and another reconstructed 30 years ago was examined in 2018. The results indicate how timber structures with wattle walls decay over a period of decades, and the evidence can be compared to that retrieved from the original excavated late prehistoric structures on the site and those found elsewhere. The pattern of wear of floors and surfaces, and the effects of decades of fires within the roundhouses, also produced archaeological signatures that can be compared with prehistoric remains. These investigations reveal for the first time how long-term processes of experimental house use, maintenance and decay in a temperate environment create archaeological signatures very similar to those found from prehistory, indicating how ancient deposits could have been formed. It also demonstrates how even roundhouses with relatively ephemeral below-ground elements can stand for at least a human generation.

The management of the remainder of Europe’s once extensive forests is hampered by a poor understanding of the character of the vegetation and drivers of change before the onset of clearance for farming. Pollen data indicate a closed-canopy, mixed-deciduous forest, contrasting with the assertion that large herbivores would have maintained a mosaic of open grassland, regenerating scrub and forested groves. Coprophilous fungal spores from sedimentary sequences are increasingly used as a proxy for past herbivore impact on vegetation, but the method faces methodological and taphonomical issues. Using pollen trap data from a long-running experiment in Chillingham Wild Cattle Park, UK, we investigate the first steps in the mechanisms connecting herbivore density to the incorporation of fungal spores in sediments and assess the effects of environmental variables on this relationship. Herbivore utilisation levels correlate with dung fungal spore abundance. Chillingham is densely populated by large herbivores, but dung fungal spore influx is low. Herbivores may thus be present on the landscape but go undetected. The absence of dung fungal spores is therefore less informative than their presence. Dung fungal spores likely enter the sediment record through a different pathway from wind-borne pollen and thus dung fungal abundance is better expressed as influx rates than as percentage of total pollen. Landscape openness, vegetation type and site wetness do not distort the impact of utilisation levels on dung fungal spore representation. However, dung fungal spore influx varies markedly between seasons and years. Spores travel, leading to a background level of spore deposition across the landscape, and at times a depletion of spores, especially under wet weather conditions. Animal behaviour, as well as husbandry practices, can lead to the accumulation of dung, and thus fungal spores, in specific locations on the landscape that do not directly reflect grazing pressure.

The 23 fossil human remains found at La Chaise-de-Vouthon Abri Bourgeois-Delaunay (Charente, Southwestern France) are all attributed to Neanderthals. The BD 1 mandible represents one of the best preserved specimens recovered in this assemblage, still bearing the entire set of 16 teeth, and attributed to an adult - likely 20-35 years old –individual. The geochronological and paleontological data indicate that the Neanderthals from Abri Bourgeois-Delaunay lived in a temperate environment between 127 and 116 ka (i.e., during the OIS 5e). In this study, we integrate and expand the morphometric record of BD 1 by quantifying the tissue proportions of its entire permanent dentition, including radicular thickness repartition of four front teeth, and comparing its degree of endostructural asymmetry in tooth tissue organization and postcanine cortical bone topography with the patterns shown by Regourdou 1. Our results show that crown and radicular dental internal signature is typically Neanderthal, comparable with the available data for Regourdou 1, and is unambiguously distinct from the fossil and extant modern human patterns. Asymmetry in tooth tissue proportions in BD 1 and Regourdou 1 is clearly non-directional, as side dominance is nearly equally distributed between the left and the right antimeres. Similarly, both Neanderthal mandibles show no marked cortical bone asymmetry at the molar level, even if a slight right dominance is found in BD 1. Even if these results are globally consistent for the two Neanderthal mandible, extensive methodological research on the patterns of age- and sex-related endostructural variation displayed by human population samples from diverse chrono-geographic, socio-economic and biocultural contexts is still needed to more confidently evaluate the evolutionary and adaptive significance of the signals from the fossil record. Les 23 restes humains fossiles découverts à La Chaise-de-Vouthon Abri Bourgeois-Delaunay (Charente, Sud-Ouest de la France) sont tous attribués à des Néandertaliens. La mandibule BD 1 représente un des spécimens les mieux préservés de l’assemblage, conservant encore ses 16 dents, et a été attribuée à un individu adulte – vraisemblablement âgé d’environ 20-35 ans. Les données géochronologiques et paléontologiques indiquent que les Néandertaliens de l’Abri Bourgeois-Delaunay vivaient dans un environnement tempéré il y a environ 127 à 116 ka (i.e., pendant l’OIS 5e). Dans cette étude, nous apportons des informations morphométriques complémentaires sur la structure de BD 1 en quantifiant les proportions des tissus de l’ensemble de ses dents permanentes, incluant la distribution d’épaisseur de dentine radiculaire de quatre dents antérieures, et en comparant le degré d’asymétrie endostructurale de l’organisation des tissus dentaires et de la topographie de l’os cortical au niveau des dents post-canines avec les patrons structuraux de Regourdou 1. Nos résultats montrent que la signature interne de la couronne et de la racine des dents est typiquement néandertalienne, comparable à celle de Regourdou 1, et clairement distincte des patrons humains modernes fossile et actuel. L’asymétrie des proportions des tissus dentaires de BD 1 et Regourdou 1 est non-directionnelle, sans dominance marquée entre les antimères gauches et droits. De la même manière, les deux mandibules néandertaliennes ne montrent pas de d’asymétrie marquée d’épaisseur de l’os cortical au niveau des molaires, même si une faible dominance droite est observée chez BD 1. Même si ces résultats sont globalement concordants pour les deux mandibules néandertaliennes, l’étude méthodologique étendue des patrons de variation de l’endostructure liés à l’âge et au sexe montrée par des populations humaines issues de divers contextes chrono-géographiques, socio-économiques et bioculturels reste nécessaire afin de mieux évaluer la valeur évolutive et adaptative des signaux observés dans le registre fossile.

The adaptive significance of adjusting behavioural activities to the right time of the day is intuitive. Laboratory studies have implicated an important role of circadian clocks in behavioural timing and rhythmicity. Yet, recent studies on clock-mutant animals questioned this importance under more naturalistic settings, as various clock mutants showed nearly normal diel activity rhythms under semi-natural Zeitgeber conditions.We here report evidence that proper timing of eclosion, a vital behaviour of the fruit fly Drosophila melanogaster, requires a functional molecular clock even under quasi-natural conditions. In contrast to wildtype flies, period01 mutants with a defective molecular clock eclose mostly arrhythmically in a temperate environment even in the presence of a full complement of abiotic Zeitgebers. Moreover, period01 mutants eclose during a much larger portion of the day, and peak eclosion time becomes more susceptible to variable day-to-day changes of light and temperature. Under the same conditions, flies with impaired peptidergic inter-clock signalling (pdf01 and han5304 mutants) stayed largely rhythmic with normal gate sizes. Our results suggest that the presence of natural Zeitgebers can mitigate a loss of peptide-mediated phasing between central clock neuron groups, but cannot substitute for the lack of a functional molecular clock under natural temperate conditions.

The objective of the three-year study was to examine spatial and temporal patterns of fluxes and soil pore air concentrations of methane (CH4), carbon dioxide (CO2) and nitrous oxide (N2O) from an experimental mass grave located in a temperate environment. The mass grave (5 × 10 m) contained twenty pig carcasses at a depth of approximately 1 m was compared to a plot of the same dimensions containing only disturbed soil, as well as an undisturbed plot. Soil pore air CH4 concentrations were sub-ambient (100,000 ppm at 1 m) in the mass grave, though there was a marked decrease from years 1 to 3. Surface fluxes of CO2 showed strong seasonal variations, peaking in summer. Soil pore air N2O concentration showed major increases in the mass grave, compared to the other two plots with the pattern maintained over the three years, resulting in larger surface fluxes of N2O. To establish the role of the carcasses in N2O dynamics, we incubated a soil sample containing carcass material which resulted in fast rates of N2O production and consumption. The maintenance of elevated pore air concentration and surface flux of N2O throughout the 3 years suggests that this is a long-term pattern and likely the best of the three gases to use to detect graves. Thus, we suggest that measurement of soil pore air concentrations, especially of N2O, could be a simple and effective approach to help determine the location of clandestine graves.

Heat acclimation over consecutive days has been shown to improve aerobic-based performance. Recently, it has been suggested that heat training can improve performance in a temperate environment. However, due to the multifactorial training demands of athletes, consecutive-day heat training may not be suitable. The current study aimed to investigate the effect of brief (8×30 min) intermittent (every 3–4 days) supplemental heat training on the second lactate threshold point (LT2) in temperate and hot conditions. 21 participants undertook eight intermittent-day mixed-intensity treadmill exercise training sessions in hot (30 °C; 50% relative humidity [RH]) or temperate (18 °C; 30% RH) conditions. A pre- and post-incremental exercise test occurred in temperate (18 °C; 30% RH) and hot conditions (30 °C; 50% RH) to determine the change in LT2. The heat training protocol did not improve LT2 in temperate (Effect Size [ES]±90 confidence interval=0.10±0.16) or hot (ES=0.26±0.26) conditions. The primary finding was that although the intervention group had a change greater than the SWC, no statistically significant improvements were observed following an intermittent eight day supplemental heat training protocol comparable to a control group training only in temperate conditions. This is likely due to the brief length of each heat training session and/or the long duration between each heat exposure. Refereed/Peer-reviewed

To mitigate the impacts of heat exposure, animals can take some actions to maintain their core body temperature, such as heat acclimation; however, the effect of heat acclimation on anxiety-like behavior in an open field is still not understood. The purpose of this study was to examine the anxiety-like behavior of heat acclimated rats in a temperate or heated open field. After being raised in a 23 °C environment for one week, male Wistar rats were exposed to a heated environment (32 °C) for 3 h (3H), 14 days (14D), or 28 days (28D), with free access to food and water, and compared with rats reared in a temperate environment (23 °C; Cont). After heat exposure, behavioral changes were monitored using an open field test (OFT) in a heated (32 °C) or temperate environment (23 °C). Compared with those in the Cont group, the body weights of rats in the 14D and 28D groups were lower. The OFT in the heated environment showed that grooming time was longer in 3H and 14D rats. In the temperate environment, grooming time was longer in all the heated groups. Rats from the 3H and 28D groups spent longer time in the center square when tested in the temperate environment. Rearing activity increased in 28D rats in the temperate environment, while the number of line crossings did not differ significantly between the heated groups and the two open fields. These results suggest that heat acclimation affected not only the physiological index such as core body temperature but also the anxiety-like behavior, mainly in the temperate open field. These changes might be beneficial when rats are faced with an open field.

Heat stroke is the most severe manifestation of heat illness. Classic heat stroke (CHS) is defined as central nervous system (CNS) dysfunction and severe hyperthermia as a consequence of heat exposure at rest and affects mostly vulnerable populations (i.e., elderly during heat waves and/or children left in vehicles). Exertional heat stroke (EHS) shares a similar definition as CHS, except it is triggered in young, otherwise healthy individuals during physical exertion in a hot or temperate environment. CHS and EHS have long been a topic of interest in physiology and have been extensively studied; yet, there are many misconceptions regarding the impact of heat on organ systems as well as the etiology that predisposes certain individuals to collapse. This chapter discusses five misconceptions that have skewed our understanding of heat stroke pathophysiology, mainly due to misinterpretation of data, conjecture that has become dogma as well as limitations in the approaches to study the condition.

There is concern about the probable recent introduction, increased prevalence and potential economic impact of rumen fluke infection of United Kingdom cattle. A study of 339 cattle slaughtered in a Scottish red meat abattoir was undertaken with the aims of describing the prevalence and geographical distribution of rumen fluke infection, estimating its effect on production, and evaluating faecal egg counts (FECs) as a tool to diagnose infection in live animals and study the epidemiology of the disease. The overall proportion of cattle consigned to the abattoir from northern United Kingdom with rumen fluke infection in the forestomachs was 0.29. Rumen flukes were distributed predominantly in the cranial sac of the rumen and adjacent to the reticular groove. Overall, a mean of 213 and median of 44 rumen flukes was identified in the forestomachs of rumen fluke-positive cattle. The mean and median FECs of animals were 26.01 and 5.20 eggs per gram (epg), respectively. There was a significant difference between the mean FECs per rumen fluke of 0.08 and 0.13 epg during summer/autumn and winter sampling periods, respectively. The overall correlation between rumen fluke FECs and the number of flukes in the forestomach was high, albeit lower in the summer/autumn than in the winter period. The sensitivities of rumen fluke FECs for the identification of flukes in the forestomach during the summer/autumn and winter sampling periods were 0.65 and 0.85, respectively. These results will aid in the interpretation of rumen fluke FECs when monitoring cattle health and production and studying the parasite's epidemiology in a temperate environment, thereby informing rational, precise and sustainable disease control.

Forensic anthropologists have traditionally relied on their knowledge and experience of the decomposition stages to make an assessment of the time since death. However, recently new and empirical methods have been developed in various regions worldwide that propose to estimate the post-mortem interval (PMI) based on the observed decomposition changes alongside important taphonomic variables. Yet despite these methods being predominantly geographic specific, a number of methods have suggested they are effective universally and it is these 'universal' methods that have been inadequately test in Australia. The current study evaluated the accuracy of two of these methods in an Australian context, specifically the Greater Western Sydney region. The protocol developed by Megyesi et al. (2005) [1] was investigated because it is commonly cited in the literature and the PMI formula created by Vass (2011) [2] was also investigated because of its 'universal' claim. Between December 2014 and March 2016, two experimental trials were undertaken during the Australian summer seasons. Sixteen adult pig carcasses were left to decompose undisturbed on a soil surface common throughout the Western Sydney region and the Megyesi et al. (2005) [1] and Vass (2011) [2] methods were applied to the remains during this period. The results showed the Megyesi et al. (2005) [1] method overestimated the known PMI of remains, whereas the Vass (2011) [2] formula underestimated the time since death of the remains in these trials. The inaccuracy may be attributed to the constants which make up the variables in these formulas and they may not reflect the values of these variables in the Western Sydney region.

Comment on: Subjective thermal strain impairs endurance performance in a temperate environment

This study examined the association between monitoring tools, training loads, and performance in concurrent heat and hypoxia (H + H) compared with temperate training environments. A randomized parallel matched-group design involved 18 well-trained male cyclists. Participants performed 12 interval sessions (3 weeks) in either H + H (32 ± 1 °C, 50% RH, 16.6% O2 normobaric hypoxia) or control (21 °C, 50% RH, 21% O2), followed by a seven-session taper (3 weeks; 21 °C, 50% RH, 21% O2), while also maintaining external training (∼ 6–10 h/week). A 20-km time trial (TT) was completed pre- and post-training block (21 °C, 50% RH, 21% O2). Before each TT and once weekly, a 4-min cycle warm-up (70% 4-min mean maximum power) was completed. Visual analog scale rating for pain, recovery, and fatigue was recorded before the warm-up, with heart rate (HREx), heart rate recovery (HRR), and rating of perceived exertion (RPEWU) recorded following. Training load was quantified using the session rating of perceived exertion (sRPE) method throughout. Overall TT improved 35 ± 47 s with moderate correlations to HRR (r = 0.49) and recovery (r = −0.55). H + H group had a likely greater reduction in HREx [ES = −0.50 (90% CL) (−0.88; 0.12)] throughout and a greater sRPE (ES = 1.20 [0.41; 1.99]), and reduction in HRR [ES = −0.37 (−0.70;−0.04)] through the overload. RPEWU was associated with weekly training load (r = 0.37). These findings suggest that recovery and HRR in a temperate environment may be used as simple measures to identify an athlete's readiness to perform. Alternatively, the relationship of RPEWU and training load suggests that perception of effort following a standardized warm-up may be a valid measure when monitoring an athlete's training response, irrespective of the training environment.

We provide estimates of the rates of natural modification of badlands pinnacles formed in soft sediments at two sites in New Zealand and assess the utility of pinnacles for constraining seismic‐hazard models. One of the sites is in a humid temperate environment, and the other is in a semiarid environment. Rates of pinnacle modification of 30–270 mm/yr (equivalent to 3–27 m/century) are estimated by comparison of images acquired one‐to‐two decades apart. The primary mode of modification is the progressive narrowing of the pinnacle column by precipitation‐induced erosion, along with consequent loss of height as the columns become thin and unstable. Additional damage may have been produced by earthquake shaking for specific pinnacles, but in general this is likely to be trivial, given that (1) estimates of the levels of peak ground acceleration (PGA) required to shake down the pinnacles at the two sites are at least a factor of 5–13 greater than the PGAs predicted or measured from regional earthquakes during the time periods of observation (in other words they would only be significantly damaged by major local earthquakes) and (2) a fragile pinnacle has actually formed in the time periods of observation. The pinnacle modification rates are rapid enough that they would not have existed one‐to‐two centuries ago or would have been of substantially different geometries. As such, pinnacles are unlikely to be useful for constraining seismic‐hazard models for return periods greater than a few decades, at least in the context of the New Zealand environment.

To understand better the adaptation strategies of intra-annual radial growth in Pinus halepensis and Pinus sylvestris to local environmental conditions, we examined the seasonal rhythm of cambial activity and cell differentiation at tissue and cellular levels. Two contrasting sites differing in temperature and amount of precipitation were selected for each species, one typical for their growth and the other represented border climatic conditions, where the two species coexisted. Mature P. halepensis trees from Mediterranean (Spain) and sub-Mediterranean (Slovenia) sites, and P. sylvestris from sub-Mediterranean (Slovenia) and temperate (Slovenia) sites were selected. Repeated sampling was performed throughout the year and samples were prepared for examination with light and transmission electron microscopes. We hypothesized that cambial rhythm in trees growing at the sub-Mediterranean site where the two species co-exist will be similar as at typical sites for their growth. Cambium in P. halepensis at the Mediterranean site was active throughout the year and was never truly dormant, whereas at the sub-Mediterranean site it appeared to be dormant during the winter months. In contrast, cambium in P. sylvestris was clearly dormant at both sub-Mediterranean and temperate sites, although the dormant period seemed to be significantly longer at the temperate site. Thus, the hypothesis was only partly confirmed. Different cambial and cell differentiation rhythms of the two species at the site where both species co-exist and typical sites for their growth indicate their high but different adaptation strategies in terms of adjustment of radial growth to environmental heterogeneity, crucial for long-term tree performance and survival. Nasl. iz nasl. zaslona. Opis vira z dne 11. 1. 2017. Bibliografija: str. 14-15. Abstract.

This investigation assessed the acute physiological and perceptual responses to wearing additional clothing during outdoor cycling to determine if this strategy could increase the heat load while training in temperate environments. Seven male cyclists (age: 32 ± 13 y, height: 179 ± 10 cm, body mass: 74 ± 10 kg, body fat percentage: 10.3 ± 1.0%) completed 2 randomized outdoor (∼17°C and ∼82% RH), 80 min cycling sessions at moderate-hard intensities (CR10 RPE = 3–5). They wore spandex shorts and a short sleeve top (CON) or additional clothing including full-length spandex pants and a ‘winter’ cycling jacket and gloves (AC). Core temperature, heart rate, sweat rate, thermal sensation and thermal comfort were measured across the trials. Moderate increases were observed in AC vs. CON for the change in mean core temperature (0.4 ± 0.3°C, effect size, ES = 1.16 ± 0.55), change in maximum core temperature (0.5 ± 0.3°C, ES = 1.07 ± 0.48) and sweat rate (0.24 ± 0.16 L.h−1, ES = 1.04 ± 0.59). A small increase in mean heart rate (3 ± 3 bpm, ES = 0.32 ± 0.28) was observed as well as a ‘very likely’ (percentage difference = 22.4 ± 7.1) and ‘most likely’ (percentage difference = 42.9 ± 11.9) increase in thermal sensation and thermal comfort, respectively, in AC vs. CON. Dressing in additional clothing while cycling outdoors in a temperate environment increased physiological strain and sensations of warmth and discomfort. Training in additional clothing during outdoor cycling represents a practical alternative to increasing the heat load of a training session.

Evidence suggests that brain temperature (TBRAIN) is a more sensitive index than the temperature of peripheral tissues for determining physical performance and thermoeffector activity. Because direct measurements of TBRAIN in exercising humans are not currently possible, several studies have been performed in rats to investigate the extent to which TBRAIN increases during exercise and whether selective brain cooling occurs in mammals that do not possess a carotid rete. These studies have reported that (1) TBRAIN increases during exercise and can reach values above 40°C when physical exertion is performed in a hot environment or during prolonged exertion (duration longer than 1 h) in a temperate environment; (2) a dorsoventral temperature gradient exists between brain regions, with the ventrally-located structures being warmer than the dorsally-located structures; (3) the observation of selective brain cooling in rats seems to depend on the brain site where temperature is measured; and (4) the exercise-induced increase in TBRAIN negatively affects physical performance at different exercise intensities and ambient temperatures.

This study examines genetic relationships among and within the South American species of Oreobolus that span the temperate and tropical Andes hotspots and represent a good case study to investigate diversification in the Páramo. A total of 197 individuals covering the distributional range of most of these species were sequenced for the nuclear ribosomal internal transcribed spacer (ITS) and 118 individuals for three chloroplast DNA regions (trnL-F, trnH-psbA and rpl32-trnL). Haplotype networks and measures of genetic diversity were calculated at different taxonomic and geographic levels. To test for possible geographic structure, a spatial analysis of molecular variance (SAMOVA) was undertaken and species relationships were recovered using a coalescent-based approach. Results indicate complex relationships among the five South American species of Oreobolus, which are likely to have been confounded by incomplete lineage sorting, though hybridization cannot be completely discarded as an influence on genetic patterns, particularly among the northern populations of O. obtusangulus and O. cleefii. We report a case of cryptic speciation in O. obtusangulus where northern and southern populations of morphologically similar individuals are genetically distinct in all analyses. At the population level, the genetic evidence is consistent with contraction and expansion of islands of Páramo vegetation during the climatic fluctuations of the Quaternary, highlighting the role of these processes in shaping modern diversity in that ecosystem.

Aim Bill size is positively correlated with average air temperature in several avian taxa. The conventional explanation for this pattern is that the poorly insulated surface area of the bill functions to dissipate excess heat in hot climates or retain heat in cold climates (Allen's rule). We hypothesize that the applicability of Allen's rule depends upon the season of critical thermal stress, which may vary by location and species. We previously found that bill size increases with high summer temperature in song sparrows (Melospiza melodia) of California, supporting the hypothesis that larger bills are an adaptation to release dry heat and minimize evaporative water loss in a hot, dry environment. Song sparrows in eastern North America face a different climatic regime, in which summers are warm but moist, and winters are cold. We predicted that this climate region would select for smaller bills to minimize heat loss. Location From coastal Delaware and Maryland to the Allegheny Plateau of the eastern USA. Methods We measured body size and bill morphology of 274 song sparrow individuals across a climatic gradient and geographical gradient. We used linear regression models to test for effects of geographical location and 30-year means of minimum winter and maximum summer temperatures and annual rainfall on bill size. Results Bill size was best predicted by, and positively correlated with, cold winter temperatures and distance from the Atlantic coast. Body size did not change substantially with distance from the coast, indicating that the pattern of bill size was not a result of allometry. Coastal birds had bills larger than predicted by a linear effect of minimum temperature. Main conclusions Collectively, these results support the hypothesis that variation in bill size reflects selection for winter heat retention rather than for summer heat dissipation. Bill size may be shaped by physiological responses to regional climates, and the season of critical thermal stress may vary geographically, even on relatively small spatial scales.

Purpose:To examine the physiological and performance responses to a heat-acclimatization camp in highly trained professional team-sport athletes.Methods:Eighteen male Australian Rules Football players trained for 2 wk in hot ambient conditions (31–33°C, humidity 34–50%). Players performed a laboratory-based heat-response test (24-min walk + 24 min seated; 44°C), a YoYo Intermittent Recovery Level 2 Test (YoYoIR2; indoor, temperate environment, 23°C) and standardized training drills (STD; outdoor, hot environment, 32°C) at the beginning and end of the camp.Results:The heat-response test showed partial heat acclimatization (eg, a decrease in skin temperature, heart rate, and sweat sodium concentration, P < .05). In addition, plasma volume (PV, CO rebreathing, +2.68 [0.83; 4.53] mL/kg) and distance covered during both the YoYoIR2 (+311 [260; 361] m) and the STD (+45.6 [13.9; 77.4] m) increased postcamp (P < .01). None of the performance changes showed clear correlations with PV changes (r < .24), but the improvements in running STD distance in hot environment were correlated with changes in hematocrit during the heat-response test (r = –.52, 90%CI [–.77; –.12]). There was no clear correlation between the performance improvements in temperate and hot ambient conditions (r < .26).Conclusion:Running performance in both hot and temperate environments was improved after a football training camp in hot ambient conditions that stimulated heat acclimatization. However, physiological and performance responses were highly individual, and the absence of correlations between physical-performance improvements in hot and temperate environments suggests that their physiological basis might differ.