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Background: Pepper (Capsicum annuum L.) is a vegetable crop of significant economic importance, but its yield and quality are severely affected by the combined stress of low temperature and low light (LL), particularly in greenhouse environments. Despite this, the physiological and molecular mechanisms underlying pepper's response to LL stress remain poorly understood. In this study, we conducted physiological and transcriptomic analyses on two pepper genotypes: Y2, a LL-sensitive genotype, and Y425, a LL-tolerant genotype. These genotypes were subjected to LL stress conditions (10 °C/5°C, 100 µmol m⁻²s⁻¹) and control (CK) conditions (28 °C/18°C, 300 µmol m⁻²s⁻¹). Results: Three days after treatment, the phenotypes of the two pepper genotypes began to show clear distinctions, with Y425 seedlings exhibiting greater root length, shoot fresh weight, and root fresh weight compared to Y2. Additionally, comparative transcriptome analysis of leaf samples from both genotypes identified a total of 13,190 differentially expressed genes (DEGs). Gene Ontology (GO) enrichment analysis revealed that genes associated with photosynthesis, osmotic stress response, reactive oxygen species response, and other GO terms potentially contribute to LL tolerance. Moreover, three key pathways involved in the response to LL stress were identified: photosynthesis-antenna proteins, zeatin biosynthesis, and circadian rhythm pathways. The key DEGs in these pathways were expressed at higher levels in Y425 as compared with Y2. Furthermore, physiological indicators such as chlorophyll fluorescence parameters, chlorophyll content, osmoregulatory substances, and antioxidant enzyme activities decreased under LL stress; however, the reduction was significantly greater in Y2 compared to Y425, further validating the molecular findings from the transcriptome analysis. Conclusion: This study identified significant physiological and transcriptomic differences in two pepper genotypes under LL stress. It highlighted key pathways and provide novel insights into the molecular and physiological mechanisms of pepper's LL tolerance. These results emphasize the importance of optimizing greenhouse conditions for better crop productivity. [ABSTRACT FROM AUTHOR]

Background: Quantitative Real-time PCR (qRT-PCR) is a powerful technique to analyze gene expression patterns by measuring the relative abundance of mRNA transcription levels. The most crucial step in obtaining accurate results of qRT-PCR is to select suitable reference genes. Water temperature is an important factor that affects various physiological processes of fish. Presently, Japanese flounder is a commercially important marine culture species and the study of its gene expression is increasing rapidly. However, the reference genes used for Japanese flounder in previous studies, especially under temperature stress, only focused on those well-known genes widely reported in vertebrates, which might not be the proper reference genes. Results: In this study, we evaluated the suitability of eight genes including ribosomal protein L6 (rpl6), ribosomal protein L9 (rpl9), delta (4)-desaturase, sphingolipid 1 (degs1), cathepsin L (ctsl), eukaryotic translation elongation factor 1 gamma (eef1g), NSA2 ribosome biogenesis homolog (nsa2), eukaryotic translation initiation factor 3, subunit E, a (eif3ea), glutamine amidotransferase class 1 domain containing 1 (gatd1) analyzed from RNA sequencing (RNA-Seq) data and two genes including β-actin (actb) and 18S rRNA ribosomal RNA (18S RNA) selected from literature to obtain the best internal controls in qRT-PCR analysis of Japanese flounder under temperature stress. The statistical analysis methods (delta-Ct, BestKeeper, geNorm, and NormFinder) were further used to determine candidate reference gene stability. Initial results showed the suitability of eight genes from RNA-Seq data, which exhibited more stable expression levels than two commonly reported reference genes. Further analysis revealed that gatd1 and rpl6 were the best reference genes in Japanese flounder exposed to temperature stress. Conclusion: This study transcriptome-wide identified reference genes in different tissues of Japanese flounder exposed to temperature stress for the first time, providing a basis for gene expression research in flatfish. [ABSTRACT FROM AUTHOR]

Given the frequent occurrence of extreme weather during the mango flowering period due to global climate change, studying the tolerance of mango pollen at different temperatures is of great significance for the introduction, breeding, flowering management, and selection of new mango varieties. This article takes 'Renong 1', 'Dashehari', 'Apple Mango' and 'Jinhuang' as the research objects, and estimated the maximum pollen germination rate (Q), pollen tube length (TL), and the basic temperatures (Tmin, Topt, and Tmax) using quadratic equations and modified bilinear models under conditions of 14-36°C. The cumulative stress response index (CSRI) was used to study the impact of temperature on pollen germination characteristics and to determine the differences among mango pollen types/genotypes. The results showed that temperature and duration significantly affected pollen vitality. Both pollen germination rate and pollen tube length increased with temperature, reaching a maximum value at 28°C(35%, 53.2µm), and then decreased with further temperature increase. Significant differences were observed in pollen germination rates and pollen tube lengths among different varieties. Specifically, 'Renong 1' had the widest temperature range for pollen germination (22-32°C) and pollen tube growth (22-34°C), while 'Apple Mango' had the narrowest pollen germination temperature range (22-30°C ). 'Dashehari' exhibited the narrowest pollen tube temperature range (24-32°C). The inhibitory effect of low temperature on pollen tube growth was greater than that of high temperature on pollen germination. 'Renong 1' exhibited the strongest resistance to both low and high temperature stresses, while 'Apple Mango' was the most sensitive. [ABSTRACT FROM AUTHOR]

Simple Summary: As poikilotherms, insects are sensitive to ambient environmental conditions; therefore, it is important to gauge how heat stress affects their survival and fitness. The leaf beetle Monolepta hieroglyphica (Motschulsky) is a key pest within farmlands in China. This study investigated the effects of different temperatures (i.e., 25, 28, 31, and 34 °C) on the survival, reproduction, feeding capacity, and antioxidant capacity of adult leaf beetles. Laboratory assays showed that elevated temperatures (i.e., 31 and 34 °C) had a negative effect on the survival and reproduction of M. hieroglyphica. As temperatures increased (25 °C to 34 °C), the feeding capacity of M. hieroglyphica decreased significantly. Lastly, superoxide dismutase (SOD), catalase (CAT), glutathione-s-transferase (GST), and peroxidases (POD) in leaf beetles were significantly affected by ambient temperature. By assessing the thermal biology of leaf beetles, these laboratory assays can provide a theoretical basis for the development of integrated pest management (IPM) programs for M. hieroglyphica. Monolepta hieroglyphica (Motschulsky) (Coleoptera: Chrysomelidae) is widely distributed in China and is polyphorous, being a major pest to cash crops, such as corn, cotton, and millet. Given the increasing severity of the greenhouse effect in recent years, we aimed to investigate the adaptability of M. hieroglyphica adults to varying temperatures. In this study, we assessed the survival, longevity, fecundity, feeding capacity, and antioxidant capacity of leaf beetle adults under laboratory conditions at 25–34 °C. Elevated temperatures (i.e., 31 and 34 °C) had (negative) impacts on M. hieroglyphica adults' survival and reproduction. Similarly, the temperature negatively affected the feeding capacity of M. hieroglyphica adults, with the impact becoming more pronounced as the temperature increased. Under the same treatment time, the SOD and CAT activity levels increased with the increase in treatment temperature. The GST activity levels showed a decreasing trend. The POD activity showed a biphasic response to increasing temperatures, first decreasing and then increasing. The above indicates that different antioxidant enzymes of M. hieroglyphica adults have different levels of sensitivity to high temperatures. In the laboratory, our work analyzes the response of M. hieroglyphica adults to temperature from ecological and physiological research perspectives and provides strategies for strengthening its subsequent integrated pest management (IPM) under conditions of global warming or extreme weather events. [ABSTRACT FROM AUTHOR]

This study aimed to evaluate the effects of using annatto oil extract in the diet of red Betta splendens females subjected to temperature stress. A total of 150 juveniles (initial weight 0.997 ± 0.038 g and 3.909 ± 0.268 cm) were used, randomly distributed in 15 aquariums (20 L). The fish were fed for 28 days with diets containing increasing levels of annatto oil extract: 0.00; two; 4; 6- and 10-mL.kg−1 diet. In the end, all individuals were submitted to a decrease in temperature and maintenance in the cold, then passed the recovery test, remaining for 6 h at low temperatures. No differences were observed regarding animal performance and survival. The oily extract did not change digestive and liver functions and skin color. The use of 2 and 10 mL promoted an increase in superoxide dismutase activity in the skin of fish submitted to a decrease in temperature. The use of 2 and 4 mL decreased catalase activity in the skin. High levels of oil extract increased gill catalase activity in temperature recovery. It is concluded that 10 ml of annatto oil extract is efficient in mitigating the effects of low-temperature stress in Betta splendens females. [ABSTRACT FROM AUTHOR]

Climatic factors play an important role in the growth and development of plants and affect agriculture. The tolerance threshold of plants for each of these factors is limited. Any change in these factors can directly and indirectly have significant effects on agricultural production. Meanwhile, temperature stress is one of the most important damaging phenomena that causes many problems for production and yield. In this research, the time of occurrence of temperature stress with a statistical period of 44 years (1980-2023) and the relationship between air temperature with yield and biomass were investigated. According to meteorological data, June, July, and August were known as the hottest months of the year. On the other hand, the most heat waves were observed in July and August in the years 1997, 2014, and 2018, which led to a decrease in the quality of the product or the loss of the plant. According to the model evaluation results, the accuracy of the model in simulating days to flowering and days to maturity was confirmed using R² (0.8 and 0.51) and NRMSE (15.36 and 7.12). Also, the model was simulated for the studied fields with deviation percentages of 1.92, 5.65, 4.94, 1.58, 0.96, and 1.49%, respectively. It showed that the model had a satisfactory performance and could be used for maize production planning. Next, the relationship between temperature, yield, and biomass was investigated, and there was a negative and significant relationship between temperature, yield, and biomass at the 99% confidence level. [ABSTRACT FROM AUTHOR]

The fabrication and the characterization of bottom-gate thin-film transistors (TFTs) utilizing aluminum-zinc-tin-oxygen (AZTO) as a channel material has been studied. The AZTO channel was deposited by radio frequency (RF) magnetron sputtering at room temperature, with varying oxygen and argon ratios. Comprehensive measurements, including transfer curve, temperature stress (TS), negative bias temperature stress (NBTS), activation energy, and density of states (DOS), were conducted to assess the electrical properties of the TFTs. The results indicated that higher oxygen concentrations during deposition led to enhanced stability of the TFTs, primarily by reducing oxygen vacancies. The study highlights the potential of oxide semiconductors, specifically AZTO, in display applications, offering high performance and efficiency. Comparative analysis revealed that AZTO exhibits properties similar to indium-gallium-zinc oxide, making it a viable alternative. The investigation into the effects of varying argon-to-oxygen ratios provides valuable insights into the fundamental processes governing the electrical behavior of AZTO-based TFTs. The calculated activation energy and DOS further elucidate the underlying mechanisms, reinforcing the material's suitability for advanced electronics and display technologies. [ABSTRACT FROM AUTHOR]

Simple Summary: Global warming, with its associated rising water temperatures and fluctuating salinity, poses a significant threat to global aquaculture, particularly for cold-water species like rainbow trout. Improving the tolerance of aquatic species to temperature is therefore essential. This study aims to investigate the molecular response mechanism of the new rainbow trout strain "Shuike No. 1" (SK) under the dual stress of temperature and salinity. Our data showed that the influence of high temperature on SK is greater than that of salinity, and the two have an obvious synergistic effect. Our findings provide valuable insights into the molecular mechanisms underpinning dual stress responses in SK, informing future breeding programs for enhanced resilience in the face of climate change. Global warming poses a significant threat to aquaculture, particularly for cold-water species like rainbow trout (Oncorhynchus mykiss). Understanding the molecular mechanisms underlying stress responses is crucial for developing resilient strains. This study investigates the dual stress of salinity and temperature response of "Shuike No. 1" (SK), a pioneering commercially bred rainbow trout strain in China, using RNA-sequencing of gill, intestine, and liver tissues from fish exposed to four treatment combinations: freshwater at 16 °C, freshwater at 25 °C, saltwater (30‰) at 16 °C, and saltwater at 25 °C. Differential gene expression analysis identified a substantial number of DEGs, with the liver showing the most pronounced response and a clear synergistic effect observed under combined high-temperature and salinity stress. Weighted gene co-expression network analysis (WGCNA) revealed stress-responsive gene modules and identified hub genes, primarily associated with gene expression, endoplasmic reticulum (ER) function, disease immunity, energy metabolism, and substance transport. Key hub genes included klf9, fkbp5a, fkbp5b, ef2, cirbp, atp1b1, atp1b2, foxi3b, smoc1, and arf1. Functional enrichment analysis confirmed the prominent role of ER stress, particularly the pathway "protein processing in the endoplasmic reticulum." Our results reveal complex, tissue-specific responses to dual stress, with high temperature exerting a stronger influence than salinity. These findings provide valuable insights into the molecular mechanisms underpinning dual stress responses in SK, informing future breeding programs for enhanced resilience in the face of climate change. [ABSTRACT FROM AUTHOR]

Simple Summary: One of the challenges currently facing the conservation of endangered plant species is adapting the methods to the changing climate and increasing global warming. This problem is primarily linked to insufficient knowledge of whether and how quickly individuals of these species can adapt to changes in their natural habitats. Thus, it is difficult to predict whether their reintroduction into a natural environment will be successful. To come a little closer to answering this question, experiments were carried out on rare Salix lapponum plants grown in the growing room to determine how the plants respond to the stress of short-term exposure to extreme temperatures in the environment. It was found that the youngest plants were the most sensitive to low temperatures, while older ones reacted strongly to heat stress. This was indicated by an increased anthocyanin content in plant tissues and guaiacol peroxidase enzyme activity, which proved to be the most informative markers among the several used ones. The knowledge of the response of Salix lapponum to heat stress will help optimize plant acclimatization methods and perhaps contribute to the effective conservation of this valuable species of wetland flora in eastern Europe. The abiotic stresses to which plants are exposed, especially in times of climate change, can result in the disruption of natural plant physiological processes. Sudden atmospheric phenomena may increase the risk of failure in protecting rare and extinction-threatened plant species by translocation. This study aimed to determine the effect of extreme ambient temperatures on the condition and physiological response of Salix lapponum plantlets used for their reintroduction into the natural habitat. Salix lapponum plants obtained by micropropagation methods at different stages of growth under laboratory conditions were subjected to a biological experiment. Plants were exposed for 12 h to temperature extremes (0 °C and 30 °C), after which the values of selected markers of the biochemical response were determined, such as photosynthetic pigments and anthocyanin content, guaiacol peroxidase and catalase activity, the presence of ROS and the RWC value. The study showed that plants at early growth stages were sensitive to low-temperature stress. In contrast, older ones showed a stronger response to high temperature, marked by an increased anthocyanin content and guaiacol peroxidase activity. It was also found that a short exposure to temperature extremes did not change the photosynthetic pigment content or catalase activity. The results of the study may be an important indication for the optimization of plant acclimatization methods in the process of their active protection by species translocation. [ABSTRACT FROM AUTHOR]

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Climatic factors play an important role in the growth and development of plants and affect agriculture. The tolerance threshold of plants for each of these factors is limited. Any change in these factors can directly and indirectly have significant effects on agricultural production. Meanwhile, temperature stress is one of the most important damaging phenomena that causes many problems for production and yield. In this research, the time of occurrence of temperature stress with a statistical period of 44 years (1980-2023) and the relationship between air temperature with yield and biomass were investigated. According to meteorological data, June, July, and August were known as the hottest months of the year. On the other hand, the most heat waves were observed in July and August in the years 1997, 2014, and 2018, which led to a decrease in the quality of the product or the loss of the plant. According to the model evaluation results, the accuracy of the model in simulating days to flowering and days to maturity was confirmed using R2 (0.8 and 0.51) and NRMSE (15.36 and 7.12). Also, the model was simulated for the studied fields with deviation percentages of 1.92, 5.65, 4.94, 1.58, 0.96, and 1.49%, respectively. It showed that the model had a satisfactory performance and could be used for maize production planning. Next, the relationship between temperature, yield, and biomass was investigated, and there was a negative and significant relationship between temperature, yield, and biomass at the 99% confidence level.

Recent studies have demonstrated regional differences in marine ecosystem C:N:P with implications for carbon and nutrient cycles. Due to strong co-variance, temperature and nutrient stress explain variability in C:N:P equally well. A reductionistic approach can link changes in individual environmental drivers with changes in biochemical traits and cell C:N:P. Thus, we quantified effects of temperature and nutrient stress on Synechococcus chemistry using laboratory chemostats, chemical analyses, and data-independent acquisition mass spectrometry proteomics. Nutrient supply accounted for most C:N:Pcell variability and induced tradeoffs between nutrient acquisition and ribosomal proteins. High temperature prompted heat-shock, whereas thermal effects via the translation-compensation hypothesis were only seen under P-stress. A Nonparametric Bayesian Local Clustering algorithm suggested that changes in lipopolysaccharides, peptidoglycans, and C-rich compatible solutes may also contribute to C:N:P regulation. Physiological responses match field-based trends in ecosystem stoichiometry and suggest a hierarchical environmental regulation of current and future ocean C:N:P.

Selective breeding is a potent method for developing strains with enhanced traits. This study compared the growth performance and stress responses of the genetically improved Abbassa Nile tilapia strain (G9; GIANT-G9) with a local commercial strain over 12 weeks, followed by exposure to stressors including high ammonia (10 mg TAN/L), elevated temperature (37 °C), and both for three days. The GIANT-G9 showed superior growth, including greater weight gain, final weight, length gain, specific growth rate, and protein efficiency ratio, as well as a lower feed conversion ratio and condition factor compared to the commercial strain. The expression of growth hormone in the brain of the GIANT‐G9 increased significantly after 6 weeks, although it slightly decreased after 12 weeks. Growth hormone receptor 1 expression also increased significantly after 6 weeks. Muscle insulin-like growth factors (igf1 and igf2) levels up-regulated significantly only after 12 weeks in the GIANT‐G9. Under stress, serum enzymes (alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase (ALP)) were significantly higher in the GIANT‐G9, while the commercial strain had lower levels. No significant changes were observed in liver ALP activity among stressed strains. Under stress, the GIANT‐G9 exhibited marked upregulation of splenic Toll-like receptors (tlr2, tlr9, tlr21), myeloid differentiation primary response protein 88 (myd88), nuclear factor kappa B (nf-κB), interleukin (il) 1β, and il6. Notably, il6 expression was higher than il1β in the spleen, with the opposite pattern in the head kidney. In response to immune stimulation, globulin levels significantly increased in the GIANT‐G9 but with similar values to the stressed commercial strain. Myostatin expression increased in the spleen of the stressed GIANT‐G9. The commercial strain exhibited the best liver catalase and superoxide dismutase activities under stress, while the GIANT‐G9 showed increased liver glutathione-S-transferase (GST) activity after exposure to ammonia and temperature stress. Serum lysozyme activity increased in the stressed commercial strain and under temperature stress in the GIANT‐G9 but decreased under other stress conditions. Overall, the stressed commercial strain demonstrated higher survivability than the stressed GIANT‐G9. The study revealed significant interactions between strains and stress factors. The GIANT‐G9 exhibited higher growth rates but lower antioxidant and immune capacities compared to the commercial strain at the juvenile stage of life and production cycle. [ABSTRACT FROM AUTHOR]

Quality fruit production in strawberry having high nutritive and bioactive properties has got challenged in the tropics and sub-tropics due to the short and unstable winter conditions. Hence, the study assessed the vegetative and reproductive behaviors, and fruit bioactive properties of strawberry upon the blend application of chitosan (Cht) and potassium (K) at six varied formulations viz., Cht @ 0.6 g/L + K @ 2.5 g/L, Cht @ 0.8 g/L + K @ 2.5 g/L, Cht @ 1.0 g/L + K @ 2.5 g/L, Cht @ 0.6 g/L + K @ 5.0 g/L, Cht @ 0.8 g/L + K @ 5.0 g/L and Cht @ 1.0 g/L + K @ 5.0 g/L along with control (no spray of Cht or K) following a randomized complete block design with three replications. Results exhibited that Cht @ 0.8 g/L + K @ 5.0 g/L treatment had the superior vegetative response along with significantly maximum number of flowers (20.67/plant) and fruits (18.00/plant), and yield (322.50 g/plant) over others. Among the fruit bioactive compounds, total flavonoid content (21.87 mg/100 g FW) and total soluble solids (10.07%) were estimated maximum in Cht @ 1.0 g/L + K @ 2.5 g/L, while total anthocyanin (105.82 mg/100 g FW) and vitamin C (58.54 mg/100 g FW) in fruits were governed by Cht @ 1.0 g/L + K @ 5.0 g/L treatment compared to control. Therefore, regulated application of chitosan and potassium can be done to improve the yield and nutritive properties of strawberry under sub-tropical conditions.

Chicken production plays a crucial role in global food security by providing essential protein to billions, especially in developing countries. However, climate change introduces significant challenges such as temperature stress, water scarcity, feed disruptions, disease prevalence, and economic impacts. This review explores these effects on poultry production, highlighting key challenges and potential solutions. Poultry production systems differ in their vulnerability and adaptability to climate change. Backyard systems, though highly vulnerable, demonstrate resilience through native breeds. Semiintensive systems face resource-related challenges but show some adaptability. Intensive systems, despite advanced technologies, struggle with heat and energy issues. Free-range systems offer natural adaptability but are exposed to outdoor risks. Organic systems prioritize sustainability but encounter difficulties with feed management and disease control. Integrated systems foster both sustainability and resilience but also face their own set of risks. To enhance resilience, essential strategies include improving housing, water management, feed efficiency, disease control, and genetic selection. Effective implementation of these strategies requires collaboration among industry stakeholders, researchers, and policymakers to ensure food security, economic stability, and environmental sustainability. This review also emphasizes climate-smart techniques, such as integrating renewable energy and optimizing waste management, to bridge knowledge gaps and promote effective practices across various poultry production systems. [ABSTRACT FROM AUTHOR]

Introduction: Extreme water temperature affects the well-being of all aquatic animals, including fish. Higher temperatures can lead to the generation of reactive oxygen species (ROS), which can induce oxidative stress and negatively impact fish health and well-being. This study investigated the protective effects of seaweed extract on growth, antioxidant status, inflammatory responses, and gut microbiota to gain a better understanding of the acclimatization ability of Nile tilapia, Oreochromis niloticus in response to oxidative stress caused by high water temperatures. Methods: Red-seaweed, Gracilaria tenuistipitata rich in polyphenols (i.e., total phenolics and flavonoids content) was considered for the preparation of the Gracilaria extract (GE) for the study. Nile tilapia were fed the GE supplemented diet along with a control diet for 42 days, followed by 14 days of temperature ramping at a rate of 1°C every two days to the desired target (35°C) and 14 days of holding at 32°C for acclimatation. Results: Nile tilapia fed the GE had a significantly higher growth performance attributed to increased muscle fiber size compared to control (p < 0.05) after the 70 days of feeding trial. Fish fed the GE diet also showed a significantly lower lipid peroxidation by decreased malondialdehyde level when compared to control (p < 0.05). Furthermore, GE diet exhibited increased red blood cell counts with the decreased number of cellular and nuclear abnormalities. The gene expression of tight junction (i.e., occludin, claudin1, ZO-1) and nrf2 (antioxidant biomarker) were upregulated, while hsp70 (related to stress response) was downregulated in fish fed the GE diet. Additionally, GE supplementation led to an increase in bacterial diversity and the abundance of phylum Firmicutes, order Lactobacillales , and genera Sphingobacterium and Prevotella in the distal gut of Nile tilapia, which are mostly considered as beneficial for fish. Conclusion: The findings suggest that GE has the potential to be used as a dietary supplement to improve health, particularly as a stress-resistant supplement in the diet for Nile tilapia. This study may help make more informed decisions for tailoring the nutrient requirements of fish in the face of climate warming. [ABSTRACT FROM AUTHOR]

On Pinus sylvestris L. trees of different ages with different phytopathological conditions, we have identified the invasive species Diplodia sapinea (Fr.) P. Karst., which is a dangerous pathogen of coniferous trees, for the first time in Central Siberia (in the parks of Krasnoyarsk). Pure cultures are isolated from pycnidia on cones; their morphological and molecular genetic characteristics correspond to the species D. sapinea. Siberian isolates are characterized by a high radial growth rate on nutrient media and are phytopathogenic against shoots of P. sylvestris and fruits of Granny Smith apples in vitro. They cause rapid and extensive necrotic lesions of the bark, shoot cambium, and fruit pulp and exhibit high peroxidase and protease enzymatic activity. Siberian D. sapinea isolates do not exceed the parameters of southern isolates in terms of morphological characteristics, enzyme activity, and phytopathogenicity. Significant differences are shown with the Dp1-23 strain of Diplodia seriata De Not isolated from pycnidia on the cones of Cupressus sempervirens L. For Siberian D. sapinea isolates, high resistance to cold stress is established in the temperature range from –8 to –31°C in a series of experiments on freezing-thawing of the mycelium. After activation, the radial growth rate of isolates on potato dextrose agar (PDA) at 24°C turn out to be higher than that of strains that were not subjected to freezing; relatively slow activation is noted at –16°C compared to other temperatures. The presence of a long endophytic phase in the species, which was discovered in Central Siberia for the first time; high adaptive capabilities; and phytopathogenicity pose a particular danger to its spread to a new territory and host plants. This requires special attention and study. [ABSTRACT FROM AUTHOR]

The commercialization of 2,4‐D (2,4‐dichlorophenoxyacetic acid) latifolicide in 1945 marked the beginning of the selective herbicide market, with this active ingredient playing a pivotal role among commercial herbicides due to the natural tolerance of monocots compared with dicots. Due to its intricate mode of action, involving interactions within endogenous auxin signaling networks, 2,4‐D was initially considered a low‐risk herbicide to evolve weed resistance. However, the intensification of 2,4‐D use has contributed to the emergence of 2,4‐D‐resistant broadleaf weeds, challenging earlier beliefs. This review explores 2,4‐D tolerance in crops and evolved resistance in weeds, emphasizing an in‐depth understanding of 2,4‐D metabolic detoxification. Nine confirmed 2,4‐D‐resistant weed species, driven by rapid metabolism, highlight cytochrome P450 monooxygenases in Phase I and glycosyltransferases in Phase II as key enzymes. Resistance to 2,4‐D may also involve impaired translocation associated with mutations in auxin/indole‐3‐acetic acid (Aux/IAA) co‐receptor genes. Moreover, temperature variations affect 2,4‐D efficacy, with high temperatures increasing herbicide metabolism rates and reducing weed control, while drought stress did not affect 2,4‐D efficacy. Research on 2,4‐D resistance has primarily focused on non‐target‐site resistance (NTSR) mechanisms, including 2,4‐D metabolic detoxification, with limited exploration of the inheritance and genetic basis underlying these traits. Resistance to 2,4‐D in weeds is typically governed by a single gene, either dominant or incompletely dominant, raising questions about gain‐of‐function or loss‐of‐function mutations that confer resistance. Future research should unravel the physiological and molecular‐genetic basis of 2,4‐D NTSR, exploring potential cross‐resistance patterns and assessing fitness costs that may affect future evolution of auxin‐resistant weeds. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Global climate change has caused rapid temperature changes in marine environments. Understanding how marine organisms respond to temperature changes can help predict their richness of future biodiversity. In this study, we examined the gene expression levels and the difference in the pathways that are responsive to acute temperature stress in low- and high-latitude populations of the shore swimming crab, Charybdis japonica. The two populations of C. japonica were exposed to low- and high-temperature stresses (15°C and 28°C) and used for transcriptome sequencing. Genetic regulatory ability changes were compared to determine the diverse response of the two crab populations to temperature change. The gene expression levels and functional enrichment analysis showed that the low-latitude crab regulated more genes (938) that were mainly enriched in DNA replication and metabolic pathways, whereas the high-latitude crab regulated less genes (309) that were mainly enriched in genetic information processing at low-temperature stress. Furthermore, the low-latitude crab regulated less genes (33) that were mainly enriched in genetic information processing, whereas the high-latitude crab regulated more genes (280) that were mainly enriched in signal transduction and cellular process at high-temperature stress. These results implied that the low-latitude population was more resilient to high-temperature stress, while the high-latitude population was more resilient to low-temperature stress. This study enhances our understanding of how different geographic C. japonica populations respond to varying temperature environments in their living zone, which could be helpful for predicting future biodiversity trends of intertidal crustaceans under global climate change. [ABSTRACT FROM AUTHOR]

Background: The type II toxin-antitoxin system consists of tightly linked genetic units that produce a toxin and its specific antitoxin. Under typical circumstances, the antitoxin neutralizes the toxic effects caused by the toxin. However, the toxin-antitoxin systems during stress regulate bacterial growth and persistence by affecting translation or DNA replication. This study aimed to assess how variations in temperature and pH stress influence the regulation of type II toxin-antitoxin gene expression within Klebsiella pneumoniae isolates. Methods: Sixty-five Klebsiella pneumoniae isolates were identified by the VITEK®2 compact system and confirmed identity by Polymerase Chain Reaction assay by amplified rpoB gene (Housekeeping gene). The isolates were subjected to Reverse Transcriptase-polymerase chain Reaction assay to determine the transcriptional variations of the type II toxin-antitoxin system genes at diverse temperature and pH values. Results: The results of the current study demonstrated higher expression levels of the antitoxin hipB gene at (37℃, 30℃, 44℃, pH 7, pH 9), mqsR toxin gene at (37℃, 30℃, pH 7, pH 5, pH 11) and relE toxin gene at (pH 5, pH 11). Conclusion: These findings suggest the possible influence of pH and temperature on toxin-antitoxin systems, which might enhance bacterial antibiotic tolerance and persistence. [ABSTRACT FROM AUTHOR]

Abiotic stress is one of the key factors harming global agriculture today, seriously affecting the growth and yield of vegetables. Pepper is the most widely grown vegetable in the world, with both high nutritional and economic values. Currently, the increase in global extreme weather events has heightened the frequency of abiotic stresses, such as drought, high and low temperatures, waterlogging, and high salt levels, which impairs pepper growth and development, leading to its reduced yield and quality. In this review, we summarize the research progress on the responses of pepper to abiotic stress in recent years in terms of physiology, biochemistry, molecular level, and mitigation measures. We then explore the existing problems and propose future research directions. This work provides a reference for the cultivation and development of new pepper varieties resistant to abiotic stress. [ABSTRACT FROM AUTHOR]

Changing climate imposes abiotic stress factors, including salinity, flood, drought, high and low temperature, and light as well as heavy metals on medicinal plants. Plants respond to stress in terms of growth and biosynthesis of metabolites. The rhizomatous genus Curcuma has been extensively used in traditional or folk medicine worldwide and its therapeutic potential relies on its phyto-constituents. A discrete array of literature depicts negative effects on growth and alterations in yield of secondary metabolites under abiotic stress. However, one complete and precise document on the response of Curcuma spp. towards abiotic stress and their molecular mechanism is lacking. Hence, this review has been prepared by pulling together the published information on the abiotic stress response as well as the future research prospects on the Curcuma spp. along with other related rhizomatous plants of the family Zingiberaceae, to obtain prospective clues on possible responses of these medicinally potential species in the verge of changing climate. We have also highlighted the stress mitigation strategies employed by these plants in the light of available literature and by comparing with information on related species of Zingiberaceae family. We have focused on the urgency of multiomics approaches to find out the genetic and biochemical strategies of Curcuma spp. to cope with stress as well as emphasized for conservation of rare and endangered species of Curcuma. This review will be helpful for plant physiologists, farmers, pharmaceutical industries, and policy makers, to implement better plans and policies regarding uninterrupted commercial yield of Curcuma secondary metabolites in changing climate. [ABSTRACT FROM AUTHOR]

The trithorax group (TrxG) complex is an important protein in the regulation of plant histone methylation. The ABSENT, SMALL, OR HOMEOTIC DISCS 1 (ASH1) gene family, as important family members of the TrxG complex, has been shown to regulate tolerance to abiotic stress and growth and development in many plants. In this study, we identified nine GhASH1s in upland cotton. Bioinformatics analysis revealed that GhASH1s contain a variety of cis-acting elements related to stress resistance and growth and development. The transcriptome expression profiles revealed that GhASHH1.A and GhASHH2.A genes expression were upregulated in flower organs and in response to external temperature stress. The results of virus-induced gene silencing (VIGS) indicated that GhASHH1.A and GhASHH2.A genes silencing reduced the ability of cotton to adapt to temperature stress and delayed the development of the flowering phenotype. We also showed that the silencing of these two target genes did not induce early flowering at high temperature (32 °C), suggesting that GhASHH1.A and GhASHH2.A might regulate cotton flowering in response to temperature. These findings provide genetic resources for future breeding of early-maturing and temperature-stress-tolerant cotton varieties. [ABSTRACT FROM AUTHOR]

Simple Summary: Temperature acclimation enables animals, especially aquatic ones, to safely survive climate fluctuations in the natural environment. The aim of our work was to study how temperature acclimation in aquaculture affects cold-water Baikal whitefish at the embryonic stage and their well-being during a heat shock (24 °C). Selected molecular markers (telomere length, telomerase activity, and expression of target genes) showed that acclimation at the early developmental stages has a positive effect on the Baikal whitefish larvae and allows them to tolerate acute temperature stress without the harmful consequences. The data obtained will improve the survival of fish and increase their plasticity under aquaculture conditions. Acclimation through the hormesis effect increases the plasticity of organisms, which has been shown for many ectothermic animals, including fish. We investigated the effect of temperature acclimation in Baikal whitefish Coregonus baicalensis (Dybowski, 1874). Telomere length, telomerase activity, and the expression of genes, whose products are involved in the regulation of telomere length and defense against reactive oxygen species, were selected to assess the state of the larvae. Acclimation and acute temperature stress (+12 °C) had no effect on telomere length, but altered telomerase activity (acclimation decreased it; stress increased it) and the levels of genes expression. Under stress, the expression of superoxide dismutase genes was increased in acclimated larvae and that of glutathione peroxidases in non-acclimated larvae, which may indicate lower reactive oxygen species formation and slower antioxidant responses in acclimated fish. The expression of some telomere-related genes was reduced under temperature stress, but the expression of the tzap and smg genes, whose products improve the control of telomere length by preventing them from lengthening or shortening, was increased in acclimated individuals. The data obtained indicate a positive effect of acclimation on the state of the Baikal whitefish larvae by remodulation of their telomerase activity and the transcriptional profile. [ABSTRACT FROM AUTHOR]

Objectives: Acute temperature stress was explored in Hippocampus abdominalis through a comprehensive RNA-seq analysis. Methods: RNA-seq was conducted on 20-day-old H. abdominalis after 24 h of temperature stress. Four experimental conditions were established: a control group (18 °C) and three temperature treatment groups (21, 24, and 27 °C). Results: Seahorse larvae were found to be unaffected by 21 °C and 24 °C and were able to survive for short periods of time during 24 h of incubation, whereas mortality approached 50% at 27 °C. The sequencing process produced 75.63 Gb of high-quality clean data, with Q20 and Q30 base percentages surpassing 98% and 96%, respectively. A total of 141, 333, and 1598 differentially expressed genes were identified in the 21, 24, and 27 °C groups vs. a control comparison group, respectively. Notably, the number of up-regulated genes was consistently higher than that of down-regulated genes across all comparisons. Gene Ontology functional annotation revealed that differentially expressed genes were predominantly associated with metabolic processes, redox reactions, and biosynthetic functions. In-depth KEGG pathway enrichment analysis demonstrated that down-regulated genes were significantly enriched in pathways related to steroid biosynthesis, terpenoid backbone biosynthesis, spliceosome function, and DNA replication. Up-regulated genes were enriched in pathways associated with the FoxO signaling pathway and mitophagy (animal). The results indicated that temperature stress induced extensive changes in gene expression in H. abdominalis, involving crucial biological processes such as growth, biosynthesis, and energy metabolism. Conclusions: This study provided key molecular mechanisms in the response of H. abdominalis to temperature stress, offering a strong basis for future research aimed at understanding and mitigating the effects of environmental stressors on marine species. [ABSTRACT FROM AUTHOR]

Allelic variation is a valuable tool for displaying high levels of polymorphism within species and is closely correlated with crop productivity. In Marathawada, there is a significant amount of phenotypic heterogeneity among sorghum landraces. However, molecular variability needs to be reevaluated in order to identify any potential barriers that can interfere with current improvement initiatives. In the current work, we used 5 SSR markers to categorize 20 genotypes of elite (Sorghum bicolor L. Moench) accession from the Marathwada region, including one standard cultivar from various agro-economic zones. According to the results of this study, 14 alleles were found among the 20 genotypes, with a PIC value that ranged from 0.37 to 0.70 and a mean of 0.44 per locus. Each locus had anything from 1 (gpsb089) and 5 (mSbCIR223), with an average of 2.80 alleles per locus. A neighbor-joining tree was constructed and showed clustering of genotypes into two groups; this indicates that there is considerable diversity in genotypes compared with advanced cultivar for desired genotype (IS1042) by using SSR markers. Results show that most diverse cultivars were IS-4564, IS18357, and IS-18381, and significant variation was also reported in IS4566 and IS18379. [ABSTRACT FROM AUTHOR]

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Background: The dramatic temperature fluctuations spurred by global warming and the accompanying extreme weather events inhibit mango growth and threaten mango productivity. Particularly, mango flowering is highly sensitive to temperature changes. The mango fruit setting rate was significantly positively correlated with pollen activity, and pollen activity was regulated by different metabolites. Methods: In this study, the in vitro pollen of two mango varieties ('Renong No.1' and 'Jinhuang'), in which sensitivity to temperature differed significantly, were subjected to different temperature stresses (15 °C, 25 °C and 35 °C), and their metabolomics were analyzed. Results: The present results showed that 775 differential metabolites were screened by liquid chromatography–mass spectrometry and divided into 12 categories. The two varieties had significant differences in metabolite expression under different temperature stresses and the effect of low temperature on 'Renong No.1' mainly focused on amino acid metabolism, while the effect on 'Jinhuang' was mainly related to glycolysis. However, under the 35 °C temperature stress, 'Renong No.1' responded by redistributing riboflavin and betaine in vivo and the most obvious metabolic pathway of 'Jinhuang' enrichment was pyrimidine metabolism, which had undergone complex main body formation and extensive regulatory processes. The changes of metabolites of different varieties under low temperature and high temperature stress were different. Among them, flavonoids or flavonoid derivatives were included in class A (216 metabolites), C (163 metabolites) and D (233 metabolites) metabolites, indicating that flavonoid metabolites had an obvious regulatory effect on mango pollen metabolism under different temperature stress. Conclusions: The present results provide valuable information for reproductive biology studies and breeding in mango, in particular, the selection and breeding of the most suitable varieties for different production areas. [ABSTRACT FROM AUTHOR]

Copyright of Acta Edulis Fungi is the property of Acta Edulis Fungi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Abstract Global warming is a leading environmental stress that reduces plant productivity worldwide. Several beneficial microorganisms reduce stress; however, the mechanism by which plant–microbe interactions occur and reduce stress remains to be fully elucidated. The aim of the present study was to elucidate the mutualistic interaction between the plant growth-promoting rhizobacterial strain SH-19 and soybeans of the Pungsannamul variety. The results showed that SH-19 possessed several plant growth-promoting traits, such as the production of indole-3-acetic acid, siderophore, and exopolysaccharide, and had the capacity for phosphate solubilisation. The heat tolerance assay showed that SH-19 could withstand temperatures up to 45 °C. The strain SH-19 was identified as P. megaterium using the 16S ribosomal DNA gene sequence technique. Inoculation of soybeans with SH-19 improved seedling characteristics under high-temperature stress. This may be due to an increase in the endogenous salicylic acid level and a decrease in the abscisic acid level compared with the negative control group. The strain of SH-19 increased the activity of the endogenous antioxidant defense system, resulting in the upregulation of GSH (44.8%), SOD (23.1%), APX (11%), and CAT (52.6%). Furthermore, this study involved the transcription factors GmHSP, GmbZIP1, and GmNCED3. The findings showed upregulation of the two transcription factors GmbZIP1 (17%), GmNCED3 (15%) involved in ABA biosynthesis and induced stomatal regulation, similarly, a downregulation of the expression pattern of GmHSP by 25% was observed. Overall, the results of this study indicate that the strain SH-19 promotes plant growth, reduces high-temperature stress, and improves physiological parameters by regulating endogenous phytohormones, the antioxidant defense system, and genetic expression. The isolated strain (SH-19) could be commercialized as a biofertilizer.

Abstract Background The Persian walnut (Juglans regia), an economically vital species within the Juglandaceae family, has seen its mitochondrial genome sequenced and assembled in the current study using advanced Illumina and Nanopore sequencing technology. Results The 1,007,576 bp mitogenome of J. regia consisted of three circular chromosomes with a 44.52% GC content encoding 39 PCGs, 47 tRNA, and five rRNA genes. Extensive repetitive sequences, including 320 SSRs, 512 interspersed, and 83 tandem repeats, were identified, contributing to genomic complexity. The protein-coding sequences (PCGs) favored A/T-ending codons, and the codon usage bias was primarily shaped by selective pressure. Intracellular gene transfer occurred among the mitogenome, chloroplast, and nuclear genomes. Comparative genomic analysis unveiled abundant structure and sequence variation among J. regia and related species. The results of selective pressure analysis indicated that most PCGs underwent purifying selection, whereas the atp4 and ccmB genes had experienced positive selection between many species pairs. In addition, the phylogenetic examination, grounded in mitochondrial genome data, precisely delineated the evolutionary and taxonomic relationships of J. regia and its relatives. We identified a total of 539 RNA editing sites, among which 288 were corroborated by transcriptome sequencing data. Furthermore, expression profiling under temperature stress highlighted the complex regulation pattern of 28 differently expressed PCGs, wherein NADH dehydrogenase and ATP synthase genes might be critical in the mitochondria response to cold stress. Conclusions Our results provided valuable molecular resources for understanding the genetic characteristics of J. regia and offered novel perspectives for population genetics and evolutionary studies in Juglans and related woody species.

Abstract Background Dermatophagoides farinae proteins (DFPs) are abundantly expressed in D. farinae; however, their functions remain unknown. Our previous transcriptome sequencing analyses revealed that the basal expression of DFP1 and DFP2 in D. farinae was high and, more importantly, upregulated under temperature stress. Therefore, DFPs were speculated to exert a temperature stress response function. Results Real-time quantitative polymerase chain reaction detection revealed that both DFP1 and DFP2 were significantly upregulated under temperature stress. Particularly, DFP1 was upregulated under cold stress. Electrophoresis of D. farinae total proteins revealed an increased abundance of DFP1 and DFP2 (40–55 kDa bands) under temperature stress, which was corroborated by the mass spectrometry results. After silencing DFP1 and DFP2 further, temperature stress led to decreases in gene expression and survival rates. Moreover, DFP1 was identified as the upstream regulator of DFP2. Conclusion This study highlights the temperature stress response functions of DFP1 and DFP2 at the mRNA and protein levels. These results provide important insights for applying DFP1 and DFP2 as potential target genes for the molecular prevention and control of D. farinae to prevent allergic diseases. The newly established methods provide methodological guidance for the study of genes with unknown functions in mites.

Background: The Persian walnut (Juglans regia), an economically vital species within the Juglandaceae family, has seen its mitochondrial genome sequenced and assembled in the current study using advanced Illumina and Nanopore sequencing technology. Results: The 1,007,576 bp mitogenome of J. regia consisted of three circular chromosomes with a 44.52% GC content encoding 39 PCGs, 47 tRNA, and five rRNA genes. Extensive repetitive sequences, including 320 SSRs, 512 interspersed, and 83 tandem repeats, were identified, contributing to genomic complexity. The protein-coding sequences (PCGs) favored A/T-ending codons, and the codon usage bias was primarily shaped by selective pressure. Intracellular gene transfer occurred among the mitogenome, chloroplast, and nuclear genomes. Comparative genomic analysis unveiled abundant structure and sequence variation among J. regia and related species. The results of selective pressure analysis indicated that most PCGs underwent purifying selection, whereas the atp4 and ccmB genes had experienced positive selection between many species pairs. In addition, the phylogenetic examination, grounded in mitochondrial genome data, precisely delineated the evolutionary and taxonomic relationships of J. regia and its relatives. We identified a total of 539 RNA editing sites, among which 288 were corroborated by transcriptome sequencing data. Furthermore, expression profiling under temperature stress highlighted the complex regulation pattern of 28 differently expressed PCGs, wherein NADH dehydrogenase and ATP synthase genes might be critical in the mitochondria response to cold stress. Conclusions: Our results provided valuable molecular resources for understanding the genetic characteristics of J. regia and offered novel perspectives for population genetics and evolutionary studies in Juglans and related woody species. [ABSTRACT FROM AUTHOR]