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Background: ST-elevation myocardial infarction (STEMI) and Takotsubo syndrome (TS) are two distinct cardiac conditions that both result in sudden loss of cardiac dysfunction and that are difficult to distinguish clinically. This study compared plasma protein changes in 24 women with STEMI and 12 women with TS in the acute phase (days 0–3 post symptom onset) and the stabilization phase (days 7, 14, and 30) to examine the molecular differences between these conditions. Methods: Plasma proteins from STEMI and TS patients were extracted during the acute and stabilization phases and analyzed via quantitative proteomics. Differential expression and functional significance were assessed. Data are accessible on ProteomeXchange, ID PXD051367. Results: During the acute phase, STEMI patients showed higher levels of myocardial inflammation and tissue damage proteins compared to TS patients, along with reduced tissue repair and anti-inflammatory proteins. In the stabilization phase, STEMI patients exhibited ongoing inflammation and disrupted lipid metabolism. Notably, ADIPOQ was consistently downregulated in STEMI patients in both phases. When comparing the acute to the stabilization phase, STEMI patients showed increased inflammatory proteins and decreased structural proteins. Conversely, TS patients showed increased proteins involved in inflammation and the regulatory response to counter excessive inflammation. Consistent protein changes between the acute and stabilization phases in both conditions, such as SAA2, CRP, SAA1, LBP, FGL1, AGT, MAN1A1, APOA4, COMP, and PCOLCE, suggest shared underlying pathophysiological mechanisms. Conclusions: This study presents protein changes in women with STEMI or TS and identifies ADIPOQ, SAA2, CRP, SAA1, LBP, FGL1, AGT, MAN1A1, APOA4, COMP, and PCOLCE as candidates for further exploration in both therapeutic and diagnostic contexts. [ABSTRACT FROM AUTHOR]

Per- and polyfluoroalkyl substances (PFAS) have become an environmental issue worldwide. A first step to assessing potential adverse effects on fish populations is to determine if concentrations of concern are present in a region and if so, in which watersheds. Hence, plasma from adult smallmouth bass Micropterus dolomieu collected at 10 sites within 4 river systems in the mid-Atlantic region of the United States, from 2014 to 2019, was analyzed for 13 PFAS. These analyses were directed at better understanding the presence and associations with land use attributes in an important sportfish. Four substances, PFOS, PFDA, PFUnA, and PFDoA, were detected in every plasma sample, with PFOS having the highest concentrations. Sites with mean plasma concentrations of PFOS below 100 ng/ml had the lowest percentage of developed landcover in the upstream catchments. Sites with moderate plasma concentrations (mean PFOS concentrations between 220 and 240 ng/ml) had low (< 7.0) percentages of developed land use but high (> 30) percentages of agricultural land use. Sites with mean plasma concentrations of PFOS > 350 ng/ml had the highest percentage of developed land use and the highest number PFAS facilities that included military installations and airports. Four of the sites were part of a long-term monitoring project, and PFAS concentrations of samples collected in spring 2017, 2018, and 2019 were compared. Significant annual differences in plasma concentrations were noted that may relate to sources and climatic factors. Samples were also collected at two sites for tissue (plasma, whole blood, liver, gonad, muscle) distribution analyses with an expanded analyte list of 28 PFAS. Relative tissue distributions were not consistent even within one species of similar ages. Although the long-chained legacy PFAS were generally detected more frequently and at higher concentrations, emerging compounds such as 6:2 FTS and GEN X were detected in a variety of tissues. [ABSTRACT FROM AUTHOR]

Increasing the planting density of mango orchards appears promising for obtaining higher yields, particularly during the first productive years. However, the challenge is to maintain a good balance between vegetative growth and fruit production in the longer term. The objective of this study was to decipher the effects of planting density, training system and cultivar on young mango trees' growth and production. The experiment, conducted in North Queensland, consisted of five combinations of planting density and training system applied to the cultivars Keitt, Calypso and NMBP-1243. The planting densities were low (208 tree ha−1), medium (416 tree ha−1) and high (1250 tree ha−1). The closed vase conventional training system was applied at each density. Single leader and espalier on trellis training systems were applied at medium and high densities, respectively. The tree canopy dimensions were measured every 6 months from planting, and tree production was recorded from the third to the fifth years after planting. Vegetative growth and fruit production were the results of complex interactions between planting density, training system, cultivar and/or time. The expected increase in orchard yield with higher planting density was observed from the first productive year, despite lower individual tree production at high planting density. Lower vegetative growth and fruit production at high planting density were probably caused by competition between trees. NMBP-1243 and Keitt showed more rapid vegetative growth. Keitt was the most productive cultivar during the first three productive years. The detailed results of this study provide avenues to further explore the behaviour of mango trees at high planting densities. [ABSTRACT FROM AUTHOR]

Net ecosystem productivity (NEP) plays an important role in the quantitative evaluation of carbon source/sinks in terrestrial ecosystems. This study used Theil–Sen median trend analysis, the Mann–Kendall method, and the Geodetector model to analyze the variation patterns and its dominant factors of NEP from 2010 to 2020. The results showed the following: (1) During 2010–2020, the spatial distribution of carbon sinks in China's terrestrial ecosystems showed a pattern of high in the southeast and low in the northwest. The area with NEP < 0 accounted for 44.74% of the total area, while the area with NEP > 0 accounted for 55.26%. (2) The northwest region belonged to the significant carbon source, while the other regions belonged to significant carbon sinks. (3) The annual average NEP in different sub-regions showed an increasing trend. During 2010–2020, the overall NEP in China showed a trend in shifting from low-level to high-level, indicating that the NEP of terrestrial ecosystems in China increased during the past 11 years. (4) The NEP gravity center in Northeast China showed a trend in moving southward and then northward, while that of the NEP gravity center in East China showed a circular migration trend of 'northwest-southwest–northeast–southeast'. The gravity center of NEP in Northwest China was moving northeastward. The migration trajectory of the NEP gravity center in Southwest China presented a "Z" shape. The change in the gravity center of NEP in the central and southern regions had a strong circuitous nature, and the overall trend was to migrate southward. (5) The combined actions of climate change and human activities were the main reason for the change in NEP in China's terrestrial ecosystem from 2010 to 2020. (6) There were obvious differences in the dominant driving factors of NEP evolution in different regions and different periods in the past 40 years. [ABSTRACT FROM AUTHOR]

Summary: Background: Vascular dysfunction is closely associated with the progression of Alzheimer's disease (AD). A critical research gap exists that no studies have explored the in vivo temporal changes of cerebrovascular alterations with AD progression in mouse models, encompassing both structure and flow dynamics at micron-scale resolution across the early, middle, and late stages of the disease. Methods: In this study, ultrasound localisation microscopy (ULM) was applied to image the cerebrovascular alterations of the transgenic female 5×FAD mouse model across different stages of disease progression: early (4 months), moderate (7 months), and late (12 months). Age-matched non-transgenic (non-Tg) littermates were used as controls. Immunohistology examinations were performed to evaluate the influence of disease progression on the β-amyloid (Aβ) load and microvascular alterations, including morphological changes and the blood–brain barrier (BBB) leakage. Findings: Our findings revealed a significant decline in both vascular density and flow velocity in the retrosplenial cortex of 5×FAD mice at an early stage, which subsequently became more pronounced in the visual cortex and hippocampus as the disease progressed. Additionally, we observed a reduction in vascular length preceding diminished flow velocities in cortical penetrating arterioles during the early stages. The quantification of vascular metrics derived from ULM imaging showed significant correlations with those obtained from vascular histological images. Immunofluorescence staining identified early vascular abnormalities in the retrosplenial cortex. As the disease advanced, there was an exacerbation of Aβ accumulation and BBB disruption in a regionally variable manner. The vascular changes observed through ULM imaging exhibited a negative correlation with amyloid load and were associated with the compromise of the BBB integrity. Interpretation: Through high-resolution, in vivo imaging of cerebrovasculature, this study reveals significant spatiotemporal dysfunction in cerebrovascular dynamics accompanying disease progression in a mouse model of AD, enhancing our understanding of its pathophysiology. Funding: This study is supported by grants from National Key Research and Development Program of China (2020YFA0908800), National Natural Science Foundation of China (12074269, 82272014, 82327804, 62071310), Shenzhen Basic Science Research (20220808185138001, JCYJ20220818095612027, JCYJ20210324093006017), STI 2030-Major Projects (2021ZD0200500) and Guangdong Natural Science Foundation (2024A1515012591, 2024A1515011342).

In tropical forest ecosystems, pastures are considered a limiting environment for dung beetles, with long‐term negative effects on the diversity of this group. To understand how dung beetle diversity changes over time in introduced Brazilian pastures, studies comparing different periods are needed. Here, we conducted a study sampling dung beetles during 48 consecutive weeks in 1995 and 2020–2021. We used pitfall traps baited with fresh cattle dung in the same introduced pasture located in the Cerrado‐Pantanal ecotone of Brazil. Our study found that the total species richness of dung beetles decreased by 40% in 2020–2021 compared to 1995, which could negatively impact the ecological functions of the pastures. Given the importance of dung beetles for maintaining and functioning of pastures, the decline in dung beetle diversity could lead to the degradation of pasture landscapes. We advocate for management plans for livestock production in introduced pastures that include the long‐term conservation of dung beetle biodiversity. These plans will ensure the continued provision of dung beetle ecological services by dung beetles over the years. [ABSTRACT FROM AUTHOR]

Literature review suggests that studies on biomedical waste generation and disposal behaviors in North America are limited. Given the infectious nature of the materials, effective biomedical waste management is vital to the public health and safety of the residents. This study explicitly examines seasonal variations of treated biomedical waste (TBMW) disposal rates in the City of Regina, Canada, from 2013 to 2022. Immediately before the onset of COVID-19, the City exhibited a steady pattern of TBMW disposal rate at about 6.6 kg∙capita−1∙year−1. However, the COVID-19 pandemic and its associated lockdowns brought about an abrupt and persistent decline in TBMW disposal rates. Inconsistent fluctuations in both magnitude and variability of the monthly TBMW load weights were also observed. The TBMW load weight became particularly variable in 2020, with an interquartile range 4 times higher than 2019. The average TBMW load weight was also the lowest (5.1 tonnes∙month−1∙truckload−1) in 2020, possibly due to an overall decline in non-COVID-19 medical emergencies, cancellation of elective surgeries, and availability of telehealth options to residents. In general, the TBMW disposal rates peaked during the summer and fall seasons. The day-to-day TBMW disposal contribution patterns between the pre-pandemic and post-pandemic are similar, with 97.5% of total TBMW being disposed of on fixed days. Results from this Canadian case study indicate that there were observable temporal changes in TBMW disposal behaviors during and after the COVID-19 lockdowns. [ABSTRACT FROM AUTHOR]

Global warming has largely advanced spring vegetation phenology, which has subsequently affected terrestrial carbon and water cycles. However, further shifts in vegetation phenology under future climate change remain unclear. We estimated the start of the growing season (SOS) by applying multiple extraction methods based on the NDVI3g data set, and then parameterized and evaluated 11 spring vegetation phenology models that included chilling, forcing, and the photoperiod. Based on scenario data from three Shared Socioeconomic Pathways (SSP126, SSP245, and SSP585) derived from eight climate models, future vegetation phenology was predicted using the phenology models. Results showed that all the phenology models performed better than the NULL model (mean of the SOS), with the performance of one‐phase models broadly matching that of two‐phase models, although the best models varied by vegetation type. The spatial pattern of simulated SOS was similar among the models, and it explained >75% of the variation. Based on the mean predicted SOS, we found that spring vegetation phenology will continue to advance under strong warming conditions (SSP245 and SSP585), but that the trend of advance will reverse at around 2060 under the SSP126 scenario. The continued trend in SOS advance is likely related to rapid forcing fulfillment under stronger warming conditions. However, under moderate warming, chilling might be reduced and it might require longer to compensate for higher forcing, which ultimately would result in SOS delay. Our findings highlight that trends will likely change under different warming conditions, potentially causing widespread impact on species interaction, biodiversity, and ecosystem function. Plain Language Summary: Phenological change, which is often seen as an indicator of climate change, has received widespread attention. However, despite this focus, comprehension of how vegetation phenology might alter under different climate change scenarios remains inadequate. We used 11 process‐based phenology models to forecast changes in the start of the growing season (SOS) in the Northern Hemisphere under 3 climate change scenarios (SSP126, SSP245, and SSP585). Results showed that the one‐phase models and the two‐phase models exhibited similar performance, which exceeded that of the NULL model (mean of the SOS). Under the strong warming scenarios (SSP245 and SSP585), spring phenology was projected to continue to advance, whereas under the moderate warming scenario (SSP126), the trend of advance was predicted to reverse at around 2060. Our findings suggest that climate change might alter the competition between species for spring resources, and thereby have widespread impact both on biodiversity and on the structure and function of ecosystems. Key Points: Process‐based models outperform statistical models in forecasting changes in spring vegetation phenology in the Northern HemisphereSimulated start of the growing season explained >75% of the percentage of varianceTrend of advance in the start of the growing season in the Northern Hemisphere will reverse by 2060 under moderate warming conditions [ABSTRACT FROM AUTHOR]

ObjectiveThe aim of this study was to assess temporal changes in physical fitness of Chinese adolescents aged 13–18 years from 1985 to 2019.MethodsBody size /composition and physical fitness indicators, including body height, weight, body mass index (BMI), speed, power, flexibility, muscular endurance, and cardiorespiratory fitness (CRF), were selected from Chinese boys and girls aged 13–18 years from eight Chinese National Surveillance on Students’ Constitution and Health from 1985 to 2019. Temporal changes in means were estimated by sample-weighted linear regression at the test × sex × age level, and national trends were estimated by a post-stratification population weighting procedure.ResultsOverall mean body height, weight and BMI increased significantly for Chinese adolescents over 34 years. There was a small improvement for boys in speed (Effect size [ES] = −0.21, 95% confidence interval [CI] = −0.44 ~ 0.02), a small improvement for boys in power (ES = 0.24, 95% CI = −0.20 ~ 0.69), a small improvement for girls in flexibility (ES = 0.45, 95% CI = 0.15 ~ 0.76), a moderate decline for boys (ES = -0.53 95% CI = −0.84 ~ −0.21) and a moderate improvement for girls (ES = 0.61, 95% CI = −0.03 ~ 1.26) in muscular endurance, and large declines in cardiorespiratory fitness (CRF) for boys (ES = 0.93, 95% CI = 0.64 ~ 1.21) and girls (ES = 0.93, 95% CI = 0.58 ~ 1.27) from 1985 to 2019. These trends in each component of fitness were more positive for adolescents aged 13–15 years than that of adolescents aged 16–18 years in both sexes, except for girls in flexibility.ConclusionThe decline in CRF was most pronounced among Chinese children and adolescents from 1985 to 2019, suggesting a future decline in population health that needs attention.

Dynamic among animal, microbiota and environment can influence the health outcome of animals. Intensive production systems, with environmental challenges and implications, are increasing important due to land limitation and market demand especially in populous regions. Analysis of temporal changes of microbiota diversity will provide baseline information and contribute to animal welfare and health management in dairy goats raised under intensive systems and influence economic outcome. The analysis of changes in microbiota population over time is made possible by employing 16S rDNA amplicon sequencing technology. Our study documented these changes in dairy goats of various physiological stages raised under the enclosed environment.

ABSTRACTThis paper challenges the traditional perspective of a territorial core–periphery pattern as an organizing principle of space by introducing a relational perspective through an empirical approach. We study spatial processes of knowledge creation among advanced producer services in Germany between 2009 and 2019. We use a unique longitudinal dataset to analyse if German regions have become more integrated into the intra-firm location networks nationally and globally, and how this translates into the processes of centralization and peripheralization. The current conceptualization of core–periphery in Germany is too static and does not reflect the economic development of the regions. We find that there is no trend of urban areas becoming more integrated and peripheral areas less integrated into these networks. Instead, our research finds a discrepancy between the federal territorial classification and the functional orientation of German regions. Regions classified as peripheral have not generally decreased in connectivity to other regions. In contrast, some have increased their connectivity and have become more central in relational terms. Overall, we identified peripheralization and centralization as a relational process with dynamic, overlapping and trans-scalar networks.

Background: ST-elevation myocardial infarction (STEMI) and Takotsubo syndrome (TS) are two distinct cardiac conditions that both result in sudden loss of cardiac dysfunction and that are difficult to distinguish clinically. This study compared plasma protein changes in 24 women with STEMI and 12 women with TS in the acute phase (days 0–3 post symptom onset) and the stabilization phase (days 7, 14, and 30) to examine the molecular differences between these conditions. Methods: Plasma proteins from STEMI and TS patients were extracted during the acute and stabilization phases and analyzed via quantitative proteomics. Differential expression and functional significance were assessed. Data are accessible on ProteomeXchange, ID PXD051367. Results: During the acute phase, STEMI patients showed higher levels of myocardial inflammation and tissue damage proteins compared to TS patients, along with reduced tissue repair and anti-inflammatory proteins. In the stabilization phase, STEMI patients exhibited ongoing inflammation and disrupted lipid metabolism. Notably, ADIPOQ was consistently downregulated in STEMI patients in both phases. When comparing the acute to the stabilization phase, STEMI patients showed increased inflammatory proteins and decreased structural proteins. Conversely, TS patients showed increased proteins involved in inflammation and the regulatory response to counter excessive inflammation. Consistent protein changes between the acute and stabilization phases in both conditions, such as SAA2, CRP, SAA1, LBP, FGL1, AGT, MAN1A1, APOA4, COMP, and PCOLCE, suggest shared underlying pathophysiological mechanisms. Conclusions: This study presents protein changes in women with STEMI or TS and identifies ADIPOQ, SAA2, CRP, SAA1, LBP, FGL1, AGT, MAN1A1, APOA4, COMP, and PCOLCE as candidates for further exploration in both therapeutic and diagnostic contexts.

Increasing the planting density of mango orchards appears promising for obtaining higher yields, particularly during the first productive years. However, the challenge is to maintain a good balance between vegetative growth and fruit production in the longer term. The objective of this study was to decipher the effects of planting density, training system and cultivar on young mango trees’ growth and production. The experiment, conducted in North Queensland, consisted of five combinations of planting density and training system applied to the cultivars Keitt, Calypso and NMBP-1243. The planting densities were low (208 tree ha−1), medium (416 tree ha−1) and high (1250 tree ha−1). The closed vase conventional training system was applied at each density. Single leader and espalier on trellis training systems were applied at medium and high densities, respectively. The tree canopy dimensions were measured every 6 months from planting, and tree production was recorded from the third to the fifth years after planting. Vegetative growth and fruit production were the results of complex interactions between planting density, training system, cultivar and/or time. The expected increase in orchard yield with higher planting density was observed from the first productive year, despite lower individual tree production at high planting density. Lower vegetative growth and fruit production at high planting density were probably caused by competition between trees. NMBP-1243 and Keitt showed more rapid vegetative growth. Keitt was the most productive cultivar during the first three productive years. The detailed results of this study provide avenues to further explore the behaviour of mango trees at high planting densities.

This paper challenges the traditional perspective of a territorial core–periphery pattern as an organizing principle of space by introducing a relational perspective through an empirical approach. We study spatial processes of knowledge creation among advanced producer services in Germany between 2009 and 2019. We use a unique longitudinal dataset to analyse if German regions have become more integrated into the intra-firm location networks nationally and globally, and how this translates into the processes of centralization and peripheralization. The current conceptualization of core–periphery in Germany is too static and does not reflect the economic development of the regions. We find that there is no trend of urban areas becoming more integrated and peripheral areas less integrated into these networks. Instead, our research finds a discrepancy between the federal territorial classification and the functional orientation of German regions. Regions classified as peripheral have not generally decreased in connectivity to other regions. In contrast, some have increased their connectivity and have become more central in relational terms. Overall, we identified peripheralization and centralization as a relational process with dynamic, overlapping and trans-scalar networks. [ABSTRACT FROM AUTHOR]

Abstract Global warming has largely advanced spring vegetation phenology, which has subsequently affected terrestrial carbon and water cycles. However, further shifts in vegetation phenology under future climate change remain unclear. We estimated the start of the growing season (SOS) by applying multiple extraction methods based on the NDVI3g data set, and then parameterized and evaluated 11 spring vegetation phenology models that included chilling, forcing, and the photoperiod. Based on scenario data from three Shared Socioeconomic Pathways (SSP126, SSP245, and SSP585) derived from eight climate models, future vegetation phenology was predicted using the phenology models. Results showed that all the phenology models performed better than the NULL model (mean of the SOS), with the performance of one‐phase models broadly matching that of two‐phase models, although the best models varied by vegetation type. The spatial pattern of simulated SOS was similar among the models, and it explained >75% of the variation. Based on the mean predicted SOS, we found that spring vegetation phenology will continue to advance under strong warming conditions (SSP245 and SSP585), but that the trend of advance will reverse at around 2060 under the SSP126 scenario. The continued trend in SOS advance is likely related to rapid forcing fulfillment under stronger warming conditions. However, under moderate warming, chilling might be reduced and it might require longer to compensate for higher forcing, which ultimately would result in SOS delay. Our findings highlight that trends will likely change under different warming conditions, potentially causing widespread impact on species interaction, biodiversity, and ecosystem function.

Net ecosystem productivity (NEP) plays an important role in the quantitative evaluation of carbon source/sinks in terrestrial ecosystems. This study used Theil–Sen median trend analysis, the Mann–Kendall method, and the Geodetector model to analyze the variation patterns and its dominant factors of NEP from 2010 to 2020. The results showed the following: (1) During 2010–2020, the spatial distribution of carbon sinks in China’s terrestrial ecosystems showed a pattern of high in the southeast and low in the northwest. The area with NEP < 0 accounted for 44.74% of the total area, while the area with NEP > 0 accounted for 55.26%. (2) The northwest region belonged to the significant carbon source, while the other regions belonged to significant carbon sinks. (3) The annual average NEP in different sub-regions showed an increasing trend. During 2010–2020, the overall NEP in China showed a trend in shifting from low-level to high-level, indicating that the NEP of terrestrial ecosystems in China increased during the past 11 years. (4) The NEP gravity center in Northeast China showed a trend in moving southward and then northward, while that of the NEP gravity center in East China showed a circular migration trend of ‘northwest-southwest–northeast–southeast’. The gravity center of NEP in Northwest China was moving northeastward. The migration trajectory of the NEP gravity center in Southwest China presented a “Z” shape. The change in the gravity center of NEP in the central and southern regions had a strong circuitous nature, and the overall trend was to migrate southward. (5) The combined actions of climate change and human activities were the main reason for the change in NEP in China’s terrestrial ecosystem from 2010 to 2020. (6) There were obvious differences in the dominant driving factors of NEP evolution in different regions and different periods in the past 40 years.

Abstract Background The global prevalence of insufficient physical activity (PA) was reported to be 27.5% in 2016, and there were stable levels of insufficient PA worldwide between 2001 and 2016. The global target of a 10% reduction in insufficient PA by 2025 will not be met if the trends remain. The relevant data for trends in China were still scarce. This study aimed to determine nationwide temporal trends in insufficient PA among adults in China from 2010 to 2018. Methods 645 903 adults aged 18 years or older were randomly selected from four nationally representative cross-sectional surveys of the China Chronic Disease and Risk Factor Surveillance conducted in 2010, 2013, 2015, and 2018. PA was measured using the Global Physical Activity Questionnaire. Temporal changes in insufficient PA prevalence and participation of domain-specific moderate- to vigorous-intensity PA (MVPA) were analyzed using logistic regression. Results From 2010 to 2018, the age-adjusted prevalence of insufficient PA in China increased from 17.9% (95% confidence interval 16.3% to 19.5%) in 2010 to 22.3% (20.9% to 23.8%) in 2018 (P for trend

Abstract Purpose To study the optical coherence tomography (OCT) changes in eyes with acute central retinal artery occlusion (CRAO) of different severity and at different disease stages. Methods The study included acute CRAO cases of

The first ornithological record from Paraná State, Southern Brazil, dates from 1820, when forests covered 83% of the area. There is a lack of studies evaluating how knowledge on species richness increased over time, together with a massive deforestation process. We aimed to describe the changes, by decade, in forest species richness since that time, and to determine how field effort hours were distributed. We reviewed published studies using online databases. We compared forest bird communities between decades with Jaccard Dissimilarity Indices and compared communities’ composition among decades with Analysis of Similarity. In 200 years, 421 forest bird species were mentioned. Most of the species were detected until 1980, a likely subset of the avifauna before habitat loss; fewer species (108) were recorded afterward. An increasing number of species (8–20) stopped being recorded by decade from the 1980s. Field effort hours were mentioned in 1984, remaining temporally and spatially uneven. More than 1,000 localities have been inventoried, but most species reports are from Eastern municipalities. Areas within Serra do Mar, Curitiba, and Londrina stand out as protected forests and well-known academic centers. Cerrado endemics corroborated the relevance of exploring different regions, but further studies in this domain are strongly recommended. [ABSTRACT FROM AUTHOR]

Advances in the diagnosis and treatment of pulmonary hypertension (PH) have gradually improved the disease course. This retrospective cohort study aims to explore the diagnostic hemodynamic profile and survival of PH patients and their temporal changes, as well as investigate potential prognostic factors. Overall, 257 adult patients were diagnosed with PH following right heart catheterization (RHC) from January 2008 to June 2023 according to the hemodynamic cut-off values proposed by the corresponding ESC/ERS guidelines at the time RHC was performed. Of these patients, 46.3% were Group 1, 17.8% Group 2, 14.0% Group 3, 18.0% Group 4, and 3.0% Group 5 PH. Temporal improvement in both diagnostic hemodynamic profile and survival of patients with PH and pulmonary arterial hypertension (PAH) was identified after 2013. Survival analysis demonstrated 5-year survival rates of 65% in Group 1 PH (90.3% in idiopathic PAH) and 77% in Group 4 PH. PAH patients being at low risk at diagnosis presented a similar 1-year all-cause mortality rate (12.4%) with high-risk ones (12.8%), primarily due to non-PH-related causes of death (62%), while high-risk patients died mostly due to PH (67%). The observed improvements in diagnostic hemodynamic profiles and overall survival highlight the importance of timely diagnosis and successful treatment strategies in PH. [ABSTRACT FROM AUTHOR]

Motor Neuron Diseases (MND) have a large clinical spectrum, being the most common amyotrophic lateral sclerosis (ALS) but there is significant clinical heterogeneity. Our goal was to investigate this heterogeneity and any potential changes during a long period. We performed a retrospective cohort study among a large Portuguese cohort of MND patients (n = 1550) and investigated changing patterns in clinical and demographic characteristics over the 27-year period of our database. With that aim, patients were divided into three 9-year groups according to the date of their first visit to our unit: P1, 1994–2002; P2, 2003–2011; P3, 2012–2020. The overall cohort's clinical and demographic characteristics are consistent with clinical experience, but our findings point to gradual changes over time. Time pattern analysis revealed statistically significant differences in the distribution of clinical phenotypes, the average age of onset, diagnostic delay, the proportin of patients using respiratory support with noninvasive ventilation (NIV), time to NIV, and survival. Across time, in the overall cohort, we found an increasing age at onset (p = 0.029), a decrease of two months in diagnostic delay (p < 0.001) and a higher relative frequency of progressive muscular atrophy patients. For ALS patients with spinal onset, from P1 to P2, there was a more widespread (54.8% vs 69.4%, p = 0.005) and earlier (36.9 vs 27.2 months, p = 0.05) use of NIV and a noteworthy 13-month increase in median survival (p = 0.041). Our results probably reflect better comprehensive care, and they are relevant for future studies exploring the impact of new treatments on ALS patients. [ABSTRACT FROM AUTHOR]

• Southern highbush blueberry stigmatic pollen loads were higher during early compared to late bloom, concordant with a decline in pollinator abundance. • Wild pollinators were only present in high numbers in the early blueberry bloom. • A higher proportion of honey bees foraged for pollen during the late bloom, which resulted in higher blueberry stigmatic pollen loads on a per bee basis. • Changes in honey bee pollen deposition may not be sufficient to compensate for the loss of wild bee pollinators during late bloom. Intra-seasonal variation in abiotic and biotic conditions can have profound consequences for pollinator community compositions and foraging movement, with flow-on effects upon pollination services. Yet, few studies have related such variations to pollination services in crop systems. In a cultivated highbush blueberry system with two primary pollinators — the managed European honey bee and a wild stingless bee species — we investigated how pollinator abundances, bee foraging behaviour, and con- and heterospecific stigmatic pollen loads changed over early, mid, and late blueberry blooming. Both con- and heterospecific stigmatic pollen loads declined following early bloom. This shift was associated with a decline in the abundance of stingless bees, whereas the abundance of honey bees only declined during late bloom. Simultaneously, honey bees were more likely to forage for blueberry pollen, and stigmatic pollen loads, relative to bee abundance, increased during late bloom. Although mixed pollen loads were common on pollinator bodies, especially on pollen foraging honey bees, heterospecific pollen deposition on blueberry stigmas was low. Given the similar effectiveness of honey bees and stingless bees as pollinators of blueberries, we contend that the observed seasonal variation in pollen deposition is likely caused by the decline in stingless bee abundances, as honey bees were not able to fully compensate for the loss of stingless bees during late bloom. Greater consideration of seasonal heterogeneity of pollinator abundance and behaviour, as part of pollination management plans, may aid in ensuring high pollination services throughout the entirety of crop bloom. [ABSTRACT FROM AUTHOR]

The COVID-19 pandemic has dramatically altered people's lives in multiple aspects, including grocery shopping behaviors. Yet, the changing trend of grocery shopping frequencies during the COVID-19 and its associations with food deserts remain unclear. We aimed to (1) examine variations of grocery shopping frequencies at county level in the USA during the COVID-19 pandemic from March 2020 to December 2021; (2) investigate associations between grocery shopping frequencies and food deserts during the COVID-19 pandemic; and (3) explore heterogeneity in grocery shopping frequencies—food desert associations across urban and rural areas. The county-level grocery shopping frequencies were derived from a grocery pattern dataset obtained from SafeGraph. We divided the 22-month period into 5 stages and employed the growth curve modeling to estimate the trajectories of grocery shopping frequencies and the associations between grocery shopping frequencies and food deserts in each stage, separately. Results revealed that grocery shopping frequencies experienced a "W-shaped" pattern from March 2020 to December 2021. Counties with the least percent of food deserts had slower decrease in grocery shopping frequencies at the initial stage and recovered more rapidly at later stages. Counties with the highest percent of food deserts were subject to deprivation amplification as a result of the pandemic. We also found differences existed in the grocery shopping frequencies—food desert associations between metropolitan counties and rural counties. Our findings suggest the impacts of COVID-19 on grocery shopping frequencies varied across different time periods, shedding light on designing different strategies to reduce the risk of contagion while shopping inside of grocery stores. Further, our findings highlight an urgent need to help people living in food deserts (especially in rural counties) to procure healthy foods safely during health emergencies like COVID-19 pandemic which disrupt mobility and social behaviors. [ABSTRACT FROM AUTHOR]

Context. Seeds harbour a diversity of microbes, which in some plants aid with germination and establishment. Seeds form a critical part in the lifecycle of plants and a role in many conservation and restoration activities. Aims. Because this is an emerging field in seed biology, we aim to highlight the key research gaps of interest to seed on the basis of restoration and ex situ conservation. Methods. We identify knowledge gaps associated with the seed endophytic microbiome of native Australian plants through undertaking a literature review. Additionally, culturing methods were used to identify the fungal seed endophytes of five native Australian species. Key results. We identified a diversity of taxa within the native seed and show three taxa that are common to all study hosts. Sampling seed from additional hosts at a site and additional sites of a host species showed new fungal diversity. Our literature review showed that little information is available on native seed microbiomes and we identified four key areas where research gaps exist, linking with seed-based restoration practices. Conclusions. We provide evidence that there is a complex and diverse seed microbiome within some Australian native plants and suggest ways that it could be integrated into restoration and conservation practices. Implications. We propose that by taking into consideration the presence of a seed microbiome and its potential impacts on plant health, seed microbiomes could be used as one method to restore microbial diversity into an ecosystem and to contribute to the seedling microbiome and plant health at restored sites. [ABSTRACT FROM AUTHOR]

Besides modern and exogenous knowledge, traditional and Indigenous knowledge of local communities are also needed for proper management and conservation of marine resources. This study aimed at assessing the knowledge of Indigenous Turkmen fishers of Gorgan Gulf which is a conservation priority coastal ecosystem in Caspian Sea, Iran. To do so, an in-field participatory research was done in five villages adjacent to Gorgan Gulf in 2020 using semi-structured interviews and focused group discussion with 48 knowledgeable Turkmen fishers through snowball sampling method. The result showed that Turkmen fishers carried extensive body of knowledge about fish species, their characteristics (size, weight, morphology, behavior), traditional fishing calendar and factors affecting it (including fish spawning time, weather condition, wind types), fishing location, orientation and navigation in fishing, fishing tools and equipment and methods of net placement. Turkmen fishers also described how the quantity, quality and diversity of fish has drastically decreased over time and they mentioned six main reasons for that including exchange of paddling and rowing boats for motor boats; adoption of galvanized nets which replaced cotton nets; decreased possibility and condition of spawning; degradation of shore habitats and decline in fish food leading to fish migration; destruction of small lakes and wetlands adjacent to Caspian Sea and overfishing. In conclusion, considering the role of small-scale fishers in human well-being and food security, their Indigenous and local knowledge must be taken into consideration for natural resources management and conservation. [ABSTRACT FROM AUTHOR]

Little is known about temporal trends of pregnant women's exposures to environmental phenols and parabens. We quantified four phenols [bisphenol A (BPA), bisphenol F, bisphenol S, and triclosan), four parabens [butyl paraben, ethyl paraben (ETPB), methyl paraben (MEPB), and propyl paraben (PRPB)], and triclocarban in 760 urine samples collected during 2007-2014 from 218 California pregnant women participating in a high-familial risk autism spectrum disorder cohort. We applied multiple regression to compute least square geometric means of urinary concentrations and computed average annual percent changes. We compared our urinary concentrations with those of other study populations to examine geographic variations in pregnant women's exposure to these target compounds. Urinary concentrations of BPA, MEPB, ETPB, and PRPB in this study population decreased over the study period [percent change per year (95% confidence interval): -5.7% (-8.2%, -3.2%); -13.0% (-18.1%, -7.7%); -5.5% (-11.0%, 0.3%); and -13.3% (-18.3%, -8.1%), respectively] and were consistently lower than those in pregnant women in other U.S. regions during the same study period. In recent years, certain phenols and parabens with known adverse health effects are being regulated or replaced with alternatives, which explains decreased body burdens observed in this study population. Either the national regulations or the advocacy campaigns in California may have influenced exposures or consumer product choices.

Abstract Background Older individuals are often underrepresented in clinical trials. In 2012 only 7% of RCT’s specifically studied older people and their geriatric characteristics were poorly reported. The aim of this review was to investigate temporal changes in characteristics and external validity of randomized controlled trials in older people from 2012 to 2019. Methods PubMed was searched for randomized clinical trials (RCTs) published in 2019. Firstly, the proportion of RCTs specially designed for older people were determined by the following criteria: a reported mean age of ≥ 70 years or a lower age cutoff of ≥ 55. Secondly, the trials with a majority of older people, defined by a reported mean age of ≥ 60 years, were screened for reporting of geriatric assessments. Both parts were compared with identical reviews performed in 2012. Results From a 10% random sample, 1446 RCTs were included in this systematic review. First, 8% of trials were specifically designed for older people in 2019 compared to 7% in 2012. Secondly, 25% of the trials included a majority of older people in 2019, compared to 22% in 2012. Thirdly, in 52% of these trials in 2019 one or more of the geriatric assessments were reported compared to 34% in 2012. Conclusions Although in 2019 the proportion of published RCTs specifically designed for older people remains low, more characteristics on geriatric assessments were reported compared to 2012. Continued efforts should be paid to increase both the number and the validity of trials for older people.

Abstract Background Low body weight is associated with an increased risk of fractures. However, the effect of temporal changes in the low body weight status on the risk of fracture remains unknown. This study aimed to evaluate the relationships between temporal changes in low body weight status and the risk of fractures in adults over the age of 40 years. Methods This study included data on adults over 40 years old who underwent two biannual consecutive general health examinations between January 1, 2007 and December 31, 2009 extracted from the National Health Insurance Database, a large nationwide population database. Fracture cases in this cohort were monitored from the time of the last health examination to the end of the designated follow-up period (from January 1, 2010 to December 31, 2018) or the participant's death. Fractures were defined as any fracture resulting in hospitalization or outpatient treatment claim after the date of general health screening. The study population was then separated into four groups based on the temporal changes in low body weight status as follows: low body weight to low body weight (L-to-L), low body weight to non-low body weight (L-to-N), non-low body weight to low body weight (N-to-L), and non-low body weight to non-low body weight (N-to-N). The hazard ratios (HRs) for new fractures, depending on weight changes over time, were calculated using Cox proportional hazard analysis. Results Adults in the L-to-L, N-to-L, and L-to-N groups had a substantially increased risk of fractures after multivariate adjustment (HR, 1.165; 95% confidence interval [CI], 1.113–1.218; HR, 1.193; 95% CI, 1.131–1.259; and HR, 1.114; 95% CI, 1.050–1.183, respectively). Although the adjusted HR was greater in participants who changed into having a low body weight, followed by those with consistently low body weight, those with low body weight remained to have an elevated risk of fracture independent of weight fluctuation. Elderly men (aged over 65 years), high blood pressure, and chronic kidney disease were significantly associated with an increase in fractures (p

[Objective] The influence of time variation of topsoil steady state infiltration rate (fc) on water movement and solute transport in the soil profile was studied in order to provide theoretical support for the study of the hydrological cycle model in this region. [Methods] The study was conducted on typical land use types near the Loess Plateau Agricultural Ecological Experimental Station of the Chinese Academy of Sciences. The fc values for a corn field, an apple orchard, and a Robinia pseudoacacia forestland were collected from April to October 2021 using the double ring infiltrometer method. [Results] ① The maximum correlation distance of fc spatial distribution was 7.12 m. ② There was a significant negative correlation between soil bulk density and fc value, while there was no significant correlation between initial soil water content and fc value (p>0.05). ③ From April to May, the fc value for each land use type increased. From June to October, the fc value for each land use type changed differently with time: fc value for the corn field increased first and then decreased, for the apple orchard increased, and for the Robinia pseudoacacia forestland increased first and then stabilized. Variance analysis showed that the fc values for different land uses were significantly affected by time (p

IntroductionEffective fisheries management requires monitoring and quantifying changes in exploited fish communities. Concerns about global fisheries sustainability have led to innovative approaches. Functional diversity, rooted in ecological theory, offers valuable insights into fishery activities and ecosystem processes. A trait-based approach was used to investigate the functional diversity of landed fish species in the Azores archipelago from 1980 to 2021.MethodsLandings data of exploited Actinopterygii and Elasmobranchii were provided by the Azores Fisheries Auction Services (LOTAÇOR/OKEANOS-UAc Fisheries Database). A trait matrix was built, incorporating 12 functional traits assigned to each species, capturing their importance in marine ecological processes. The Quickhull algorithm for convex hull was employed to calculate the volume occupied by the species in the four-dimensional functional space. Functional diversity (FD) was measured using three indices: functional richness (FRic), functional evenness (FEve), and functional divergence (FDiv). Trends in FD indices over the past 42 years were visualized using Generalized Additive Models (GAM) with interaction terms.Results and discussionGAM analysis revealed significant variations in the functional space and FD metrics over time. FRic exhibited peaks in the 1980s and 2010s, declining in the 1990s and from the 2010s onwards, indicating diversification in target species. The recent decrease in FRic can be attributed to the absence of catches of species with unique traits. The distribution of landings and trait combinations showed higher regularity in the functional space during the 1980s and 1990s (high FEve). Actinopterygii species targeted in the 1980s and 1990s had lower trait divergence (low FDiv) compared to those targeted from the 2000s onwards (high FDiv). Variability in FD can be linked to changes in fishing practices, species availability, market demand, environmental factors, and local regulations. This study underscores the importance of considering FD metrics alongside species richness and abundance when assessing the potential effects of fisheries on marine ecosystems and sustainable use of fishery resources.

ABSTRACT The microbiome profiles of poultry production systems significantly impact bird health, welfare, and the environment. This study investigated the influence of broiler-rearing systems on the microbiome composition of commercial and backyard chicken farms and their environment over time. Understanding these effects is vital for optimizing animal growth, enhancing welfare, and addressing human and environmental health implications. We collected and analyzed various samples from commercial and backyard farms, revealing significant differences in microbial diversity measurements between the two systems. Backyard farms exhibited higher alpha diversity measurements in soil and water samples, while commercial farms showed higher values for litter and feeder samples. The differences in microbial diversity were also reflected in the relative abundance of various microbial taxa. In backyard farms, Proteobacteria levels increased over time, while Firmicutes levels decreased. Campilobacterota, including the major poultry foodborne pathogen Campylobacter, increased over time in commercial farm environments. Furthermore, Bacteroides, associated with improved growth performance in chickens, were more abundant in backyard farms. Conversely, pathogenic Acinetobacter was significantly higher in backyard chicken fecal and feeder swab samples. The presence of Brevibacterium and Brachybacterium, associated with low-performing broiler flocks, was significantly higher in commercial farm samples. The observed differences in microbial composition and diversity suggest that farm management practices and environmental conditions significantly affect poultry health and welfare and have potential implications for human and environmental health. Understanding these relationships can inform targeted interventions to optimize poultry production, improve animal welfare, and mitigate foodborne pathogens and antimicrobial resistance risks. IMPORTANCE The microbiome of poultry production systems has garnered significant attention due to its implications on bird health, welfare, and overall performance. The present study investigates the impact of different broiler-rearing systems, namely, commercial (conventional) and backyard (non-conventional), on the microbiome profiles of chickens and their environment over time. Understanding the influence of these systems on microbiome composition is a critical aspect of the One-Health concept, which emphasizes the interconnectedness of animal, human, and environmental health. Our findings demonstrate that the type of broiler production system significantly affects both the birds and their environment, with distinct microbial communities associated with each system. This study reveals the presence of specific microbial taxa that differ in abundance between commercial and backyard poultry farms, providing valuable insights into the management practices that may alter the microbiome in these settings. Furthermore, the dynamic changes in microbial composition over time observed in our study highlight the complex interplay between the poultry gut microbiome, environmental factors, and production systems. By identifying the key microbial players and their fluctuations in commercial and backyard broiler production systems, this research offers a foundation for developing targeted strategies to optimize bird health and welfare while minimizing the potential risks to human and environmental health. The results contribute to a growing body of knowledge in the field of poultry microbiome research and have the potential to guide future improvements in poultry production practices that promote a sustainable and healthy balance between the birds, their environment, and the microbial communities they host.

Temporal variation of soil organic carbon (SOC) is driven by land use/management practice, ecosystem conditions and climatic variation. Robust quantification of changes in SOC that is cost-effective and provides a statistical assessment of uncertainty is challenging, particularly in the face of large spatial variability and slow soil SOC changes. Remote-sensing indicators of above-ground vegetation provide some indication of the amount of fresh organic material being supplied to the soil. Although, because of the time taken for this organic material to decay and become incorporated into the soil, there will be a lag between the changes in the indicator of vegetation growth and the resulting changes in SOC. In this work, we investigate how a remotely sensed indicator of vegetation cover can be used with a lag period to predict or indicate changes in SOC for grazed pasture sites at a long-term monitoring study, which has been monitoring soil under different land uses for over forty years. We assessed how well this worked for indicating the SOC changes for different depths in the soil profile. Results suggested that a lagged remotely sensed vegetation cover—the average cover of the two preceding years—provides some indication of SOC changes for the 0–10 cm soil depth, but changes for deeper soil depths were not well predicted. Further, we investigated the potential of using soil data from a point-in-time spatial dataset (e.g. data from a baseline sampling round) to calibrate a relationship between the remotely sensed cover and SOC, which can then be applied to predict or indicate the temporal variation of SOC. Results showed this approach gave large prediction errors, likely because the temporal variation (at a fixed point in space) and spatial variation (for a fixed point in time) of SOC that is predictable by cover differences are not interchangeable.

Flooding in low-lying coastal zones arises from coastal (storm surge, tides, and waves), fluvial (excessive river discharge), and pluvial (excessive surface runoff) drivers. We analyse changes in compound flooding potential around the contiguous United States (CONUS) coastline stemming from select combinations of these flooding drivers using long observational records with at least 55 years of data. We assess temporal changes in the tail (extremal) dependence (χ) using a 30-year sliding time window. Periods of strong tail dependence are found for the windows centered between the 1960s and 1980s/1990s at several locations for surge-discharge (S-Q) and surge-precipitation (S–P) combinations. Changes in dependence are associated with large-scale climate indices such as the Arctic Oscillation (AO) and El Nino Southern Oscillation indices (Niño 1.2 and Niño 3), among others. The significance of potential changes in the dependence structure is subsequently tested using Kullback–Leibler (KL) divergence. We find that changes are mostly not significant. Finally, we perform a complete multivariate statistical analysis exemplarily for one selected pair of variables at one location (S-Q in Washington, DC), allowing for varying dependence strength and structure as well as changes in the marginal distributions. Combined changes with increase in the dependence and marginals exacerbate the predicted compound flood potential. The comprehensive analysis presented here provides new insights into how and where compound flooding potential has changed with time, demonstrates associated links with large-scale climate indices, and highlights the effects of changes in the dependence and marginals in a multivariate statistical framework.

Yang & Song (2022, https://doi.org/10.1029/2022GL098393) first claimed existence of Earth's inner core differential rotation based on the waveform similarity of two neighboring stations AAK and KZA across an earthquake doublet and then postulated a local velocity gradient at the top of the inner core based on the difference of PKiKP‐PKIKP differential times between the stations and inferred inner core differential rotation rate. In this comment, we collectively analyze the seismic data in the region and add the data of another nearby station HORS into analysis. HORS and KZA, located in an opposite direction away from AAK, consistently exhibit high waveform similarity. Collective analysis of seismic data demonstrates the invalidity of both their logic of claiming existence of inner core differential rotation and their postulation of "a local inner core gradient" to infer differential rotation. Localized and episodic inner core surface change provides a physically consistent explanation to the seismic data. Plain Language Summary: Whether Earth's inner core differentially rotates with respect to the rest of Earth or it experiences localized episodic change of its surface has been vigorously debated. While both mechanisms are derived based on temporal changes of compressional seismic waves that touch the inner core, the hypothesis of inner core differential rotation is criticized for its lack of direct supporting seismic evidence and existence of many inconsistencies in explaining the seismic data. Yang & Song (2022, https://doi.org/10.1029/2022GL098393) claimed existence of inner core differential rotation based on waveform similarity of two neighboring stations AAK and KZA across an earthquake doublet. They further postulated a local inner core velocity gradient and inferred inner core differential rotation rate. Here, we collectively analyze the seismic data in the region and add the seismic data of another nearby station HORS into analysis. Collective analysis of seismic data demonstrates the invalidity of both their logic of claiming existence of inner core differential rotation and their postulation of "a local inner core gradient" to infer inner core differential rotation. Seismic evidence is contradictory to the hypothesis of inner core differential rotation. Instead, a localized and episodic inner core surface change provides a physically consistent explanation to the seismic data. Key Points: Yang and Song (2022)'s logic of claiming existence of inner core differential rotation is invalidYang and Song (2022)'s postulation of "a local inner core gradient" and inference of inner core differential rotation are invalidLocalized and episodic inner core surface change provides a physically consistent explanation to the seismic data [ABSTRACT FROM AUTHOR]

While neonatal mortality is a critical measure of national health and well-being, efforts to reduce it in post-colonial, global south national contexts continue to yield unsatisfactory (sometimes worsening) odds of such events. This paper applies the intersectionality framework and dependency theory to time-based changes in neonatal mortality in Tanzania from 1991 to 2016 as a new model for understanding these persistent odds of neonatal mortality in the underdeveloped world. Analysis of data from the Tanzania Demographic Health Survey (from 1991 to 2016) discloses an unambiguous intersection between residence, region, and socioeconomic status in Tanzania. At the national level, neonatal mortality decreased slightly between 1991 and 2016. However, the likelihood of neonates dying increased during that time for women living in rural and unprivileged areas with lower socioeconomic status. An intersectionality framework and dependency theory contextualize these findings by considering structural elements within Tanzania from 1991 to 2016. This new model affords fresh insights, recommendations, and policy discussion for reducing neonatal mortality in Tanzania and other post-colonial, global south nations. [ABSTRACT FROM AUTHOR]

Background: The global prevalence of insufficient physical activity (PA) was reported to be 27.5% in 2016, and there were stable levels of insufficient PA worldwide between 2001 and 2016. The global target of a 10% reduction in insufficient PA by 2025 will not be met if the trends remain. The relevant data for trends in China were still scarce. This study aimed to determine nationwide temporal trends in insufficient PA among adults in China from 2010 to 2018. Methods: 645 903 adults aged 18 years or older were randomly selected from four nationally representative cross-sectional surveys of the China Chronic Disease and Risk Factor Surveillance conducted in 2010, 2013, 2015, and 2018. PA was measured using the Global Physical Activity Questionnaire. Temporal changes in insufficient PA prevalence and participation of domain-specific moderate- to vigorous-intensity PA (MVPA) were analyzed using logistic regression. Results: From 2010 to 2018, the age-adjusted prevalence of insufficient PA in China increased from 17.9% (95% confidence interval 16.3% to 19.5%) in 2010 to 22.3% (20.9% to 23.8%) in 2018 (P for trend < 0.001). By age group, with a significant increase in insufficient PA in adults aged 18–34 years (P for trend < 0.001), which rose more rapidly than in adults aged ≥ 35 years (P for interaction < 0.001). Insufficient PA has increased significantly among adults engaged in agriculture-related work, non-manual work, and other manual work (all P for trend < 0.05). And among the occupational groups, those engaged in agriculture-related work had the fastest increase (P for interaction = 0.01). The percentage of adults participating in work-related MVPA decreased from 79.6% (77.8% to 81.5%) to 66.8% (64.9% to 68.7%) along with a decrease in time spent on work-related MVPA, while percentages of adults participating in recreation-related MVPA increased from 14.2% (12.5% to 15.9%) to 17.2% (16.0% to 18.4%) (all P for trend < 0.05). Conclusions: Among Chinese adults, an increasing trend was found in insufficient PA from 2010 to 2018, with more than one-fifth of adults failing to achieve the recommendation of adequate PA. More targeted PA promotion strategies should be developed to improve population health. [ABSTRACT FROM AUTHOR]

Seed fungal endophytes play a crucial role in assisting the overall health and success of their host plant; however, little is known about the factors that influence the diversity and composition of these endophytes, particularly with respect to how they change over time and within urban environments. Using culturing techniques, morphological analyses, and Sanger sequencing, we identified the culturable seed fungal endophytes of Banksia ericifolia at two urban and two natural sites in Sydney, New South Wales, Australia. A total of 27 Operational Taxonomic Units were obtained from 1200 seeds. Older cones were found to contain, on average, more colonised endophytes than younger cones. Species richness was also significantly influenced by cone age, with older cones being more speciose. Between urban and natural sites, the overall community composition did not change, although species richness and diversity were greatest at urban sites. Understanding how these endophytes vary in time and space may help provide an insight into the transmission pathways used and the potential role they play within the development and survival of the seed. This knowledge may also be crucial for restoration purposes, especially regarding the need to consider endophyte viability in ex situ seed collection and storage in seed-banking practices. [ABSTRACT FROM AUTHOR]

Purpose: To study the optical coherence tomography (OCT) changes in eyes with acute central retinal artery occlusion (CRAO) of different severity and at different disease stages. Methods: The study included acute CRAO cases of < 7 days duration, imaged on OCT at various time points. Based on the OCT findings at presentation, cases were classified into three severity groups: mild, moderate, and severe. OCT scans were evaluated and classified into four-time intervals based on symptom duration. Results: There were 39 eyes from 38 patients with acute CRAO who underwent 96 OCT scans. At presentation, the study had 11, 16, and 12 cases of mild, moderate, and severe CRAO, respectively. Middle retinal layer opacification was more common in mild CRAO cases, which caused inner retinal layer thinning over time. Moderate CRAO cases had total inner retinal layer opacification, which resulted in retinal thinning over time. Prominent middle limiting membrane (p-MLM) sign was seen in mild and moderate CRAO eyes while were not visualised in severe CRAO. This sign gradually faded out over time. Other OCT findings in higher grades of CRAO included inner retinal fluid, neurosensory detachment, internal limiting membrane detachment, hyperreflective foci, and posterior vitreous opacities. Regardless of the CRAO grade, the final end-point seen was inner retinal layer thinning over time. Conclusion: OCT in CRAO is a useful for determining the severity of retinal ischemia, disease stage, tissue damage mechanism, and final visual outcome. More prospective studies analysing a larger number of cases at fixed time points will be required in the future. Trial Registration: Trial Registration Number: Not applicable. [ABSTRACT FROM AUTHOR]

Understanding habitat use and nursery areas of larval fish is a key component to managing and conserving riverine fishes. Yet, freshwater researchers often focus only on adult fishes, resulting in a limited understanding of the habitat requirements for the early life stages of freshwater fishes. The goal of this study was to quantify the larval fish microhabitat use of three fish families in Twelvemile Creek, a fifth-order tributary of Lake Hartwell (Savannah River basin) in the Piedmont ecoregion of South Carolina, USA. We used handheld dipnets to sample larval fishes along 20 equidistant transects spaced 10 m apart weekly from May to July 2021 along a 200 m stream reach. We also collected microhabitat data at each larval fish capture location. Most captured individuals were in the metalarval stage and were identified to the family level. A partial distance-based redundancy analysis indicated that water velocity contributed to changes in larval fish assemblage structure. Larval fishes occupied a subset of the available habitat that was characterized by low water velocity, non-Podostemum substrate, and shallow habitats close to the shore or bed rock structure. We also detected temporal patterns in larval fish counts, with peak Percidae and Leuciscidae counts in late July and the highest Catostomidae counts in late May–early June. Our results suggest that larval fishes select habitats with low water velocity and shallow habitats close to shore microhabitat characteristics, and that riffle-pool sequences may serve as a nursery habitat for Percidae, Catostomidae and Leuciscidae metalarvae.

Background: Low body weight is associated with an increased risk of fractures. However, the effect of temporal changes in the low body weight status on the risk of fracture remains unknown. This study aimed to evaluate the relationships between temporal changes in low body weight status and the risk of fractures in adults over the age of 40 years. Methods: This study included data on adults over 40 years old who underwent two biannual consecutive general health examinations between January 1, 2007 and December 31, 2009 extracted from the National Health Insurance Database, a large nationwide population database. Fracture cases in this cohort were monitored from the time of the last health examination to the end of the designated follow-up period (from January 1, 2010 to December 31, 2018) or the participant's death. Fractures were defined as any fracture resulting in hospitalization or outpatient treatment claim after the date of general health screening. The study population was then separated into four groups based on the temporal changes in low body weight status as follows: low body weight to low body weight (L-to-L), low body weight to non-low body weight (L-to-N), non-low body weight to low body weight (N-to-L), and non-low body weight to non-low body weight (N-to-N). The hazard ratios (HRs) for new fractures, depending on weight changes over time, were calculated using Cox proportional hazard analysis. Results: Adults in the L-to-L, N-to-L, and L-to-N groups had a substantially increased risk of fractures after multivariate adjustment (HR, 1.165; 95% confidence interval [CI], 1.113–1.218; HR, 1.193; 95% CI, 1.131–1.259; and HR, 1.114; 95% CI, 1.050–1.183, respectively). Although the adjusted HR was greater in participants who changed into having a low body weight, followed by those with consistently low body weight, those with low body weight remained to have an elevated risk of fracture independent of weight fluctuation. Elderly men (aged over 65 years), high blood pressure, and chronic kidney disease were significantly associated with an increase in fractures (p < 0.05). Conclusion: Individuals aged over 40 years with low body weight, even after regaining normal weight, had an increased risk of fracture. Moreover, having a low body weight after having a normal body weight increased the risk of fractures the most, followed by those with consistently low body weight. [ABSTRACT FROM AUTHOR]

Background: Older individuals are often underrepresented in clinical trials. In 2012 only 7% of RCT's specifically studied older people and their geriatric characteristics were poorly reported. The aim of this review was to investigate temporal changes in characteristics and external validity of randomized controlled trials in older people from 2012 to 2019. Methods: PubMed was searched for randomized clinical trials (RCTs) published in 2019. Firstly, the proportion of RCTs specially designed for older people were determined by the following criteria: a reported mean age of ≥ 70 years or a lower age cutoff of ≥ 55. Secondly, the trials with a majority of older people, defined by a reported mean age of ≥ 60 years, were screened for reporting of geriatric assessments. Both parts were compared with identical reviews performed in 2012. Results: From a 10% random sample, 1446 RCTs were included in this systematic review. First, 8% of trials were specifically designed for older people in 2019 compared to 7% in 2012. Secondly, 25% of the trials included a majority of older people in 2019, compared to 22% in 2012. Thirdly, in 52% of these trials in 2019 one or more of the geriatric assessments were reported compared to 34% in 2012. Conclusions: Although in 2019 the proportion of published RCTs specifically designed for older people remains low, more characteristics on geriatric assessments were reported compared to 2012. Continued efforts should be paid to increase both the number and the validity of trials for older people. [ABSTRACT FROM AUTHOR]

Background: ECG is the initial diagnostic tool that in combination with typical symptoms often raises the suspicion of pericarditis. Echocardiography remains the first-line imaging modality for assessment of pericardial diseases, particularly effusion/tamponade, constrictive physiology, and assessment of regional wall motion abnormalities as differential diagnoses. However, cardiac CT and cardiac magnetic resonance may be necessary in complicated cases and to identify pericardial inflammation in specific settings (atypical presentation, new onset constriction), as well as myocardial involvement and monitoring the disease activity. Summary: In acute pericarditis, the most commonly used ECG criteria recommended by international guidelines are the widespread ST-segment elevation or PR depression. However, the classic ECG pattern of widespread ST-segment elevation or PR depression can be seen in less than 60% of patients. In addition, ECG changes are often temporally dynamic, evolve rapidly during the course of disease, and may be influenced by a number of factors such as disease severity, time (stage) of presentation, degree of myocardial involvement, and the treatment initiated. Overall, temporal dynamic changes on ECG during acute pericarditis or myopericarditis have received limited attention. Hence, the aim of this brief clinical review was to increase awareness about the various ECG changes observed during the course of acute pericarditis. Key Messages: ECG may be normal at presentation or for days after the index episode of chest pain, but serial ECGs can reveal specific patterns of temporally dynamic ST elevation in patients with pericarditis or myopericarditis, particularly during new episodes of chest pain. [ABSTRACT FROM AUTHOR]

Optimal management of water resources requires accurate determination of water balance components in each region and Evapotranspiration is one of the most important components of water balance. The purpose of this study was to investigate the spatiotemporal variability of reference evapotranspiration in Lorestan province-western Iran country using the Man-Kendall test and GIS then assess the effect of different climatic parameters on ET0 using multivariate regression. Lorestan province with a 28064 km area in western Iran has 9 synoptic stations including Khorramabad, Boroujerd, Aligouderz, Azna, Doroud, Koohdasht, Poldokhter, Aleshatar, and Noorabad. In this study, meteorological data were used for 9 synoptic stations of the Lorestan in a period from 2001 to 2017. The results showed that at most of the stations and most months, the changing trend was decreasing. The annual decrease in Azna station with Z=-2.73 at 99% level, and in the stations of Aligodarz, Kohdasht, and Doroud with Z equal to -2.27, -2.35, and -2.2, respectively at 95% was significant. The spatial distribution of ET0 showed that the maximum amount of ET0 occurred in the south of Lorestan Province, and decreased from south to north and west to east of the study area. These results indicate the influence of latitude and altitude on the spatial distribution of ET0. The impact of different parameters showed the greatest effect of maximum temperature and wind speed on ET0. [ABSTRACT FROM AUTHOR]

Diatoms of the genus Pseudo-nitzschia are producers of the neurotoxin domoic acid that causes serious damage to marine fauna and aquaculture farms. These microalgae are known as the most abundant group of toxic phytoplankton in Peter the Great Bay, Northwestern Sea of Japan, during the bloom season, which, as was previously reported for members of this group in the study region, lasts in the autumn months. Autumnal blooms of toxic diatoms Pseudo-nitzschia spp. were studied in the period from 2017 to 2022 in Ussuri Bay, the largest body of water in Peter the Great Bay, which harbors numerous recreational area and aquaculture farms. As a result, the following changes in the composition of bloom-forming species were recorded: blooms were caused by the Pseudo-nitzschia delicatissima group in the period from 2017 to 2020 and by P. multistriata in 2021–2022. An assumption has been made that one of the factors responsible for blooms of P. multistriata, known as one of the most widespread species in Asian warm-water areas, is an abnormally high water temperature in the autumn of 2021 in the study area. [ABSTRACT FROM AUTHOR]

Bird observation mainly relies on field surveys, which are time-consuming and laborious. In this study, we explored using street-view images in the virtual survey of urban birds and nests. Using the coastal city of Qingdao as the study area, 47 201 seamless spherical photos at 2741 sites were collected using the Baidu street-view (BSV) map. Single-rater-all photo checks and seven-rater-metapopulation checks were used to find inter–rater repeatability, the best viewing layer for BSV collection, and possible environments affecting the results. We also collected community science data for comparison. The BSV time machine was used to assess the temporal dynamics. Kappa square test, generalized linear model, redundancy ordination and ArcMap were used in the analysis. Different rater repeatability was 79.1% in nest evaluations and 46.9% in bird occurrence. A re-check of the different-rating photos can increase them to 92% and 70%. Seven-rater statistics showed that more than 5% sampling ratio could produce a non-significant different bird and nest percentage of the whole data, and the higher sampling ratio could reduce the variation. The middle-viewing layer survey alone could produce 93% precision of the nest checks by saving 2/3 of the time used; in birds, selecting middle and upper-view photos could find 97% of bird occurrences. In the spatial distribution, the nest's hotspot areas from this method were much greater than the community science bird-watching sites. The BSV time machine made it possible to re-check nests in the same sites but challenging the re-check of bird occurrences. The nests and birds can be observed more in the leafless season, on wide, traffic-dense coastal streets with complex vertical structures of trees, and in the gaps of tall buildings dominated by road forests. Our results indicate that BSV photos could be used to virtually evaluate bird occurrence and nests from their numbers, spatial distribution and temporal dynamics. This method provides a pre-experimental and informative supplement to large-scale bird occurrence and nest abundance surveys in urban environments. [ABSTRACT FROM AUTHOR]

Divergence in the face of high dispersal capabilities is a documented but poorly understood phenomenon. The white‐tailed eagle (Haliaeetus albicilla) has a large geographic dispersal capability and should theoretically be able to maintain genetic homogeneity across its dispersal range. However, following analysis of the genomic variation of white‐tailed eagles, from both historical and contemporary samples, clear signatures of ancient biogeographic substructure across Europe and the North‐East Atlantic is observed. The greatest genomic differentiation was observed between island (Greenland and Iceland) and mainland (Denmark, Norway and Estonia) populations. The two island populations share a common ancestry from a single mainland population, distinct from the other sampled mainland populations, and despite the potential for high connectivity between Iceland and Greenland they are well separated from each other and are characterized by inbreeding and little variation. Temporal differences also highlight a pattern of regional populations persisting despite the potential for admixture. All sampled populations generally showed a decline in effective population size over time, which may have been shaped by four historical events: (1) Isolation of refugia during the last glacial period 110–115,000 years ago, (2) population divergence following the colonization of the deglaciated areas ~10,000 years ago, (3) human population expansion, which led to the settlement in Iceland ~1100 years ago, and (4) human persecution and exposure to toxic pollutants during the last two centuries. [ABSTRACT FROM AUTHOR]