119 results on '"Tengfei Ji"'
Search Results
2. Author correction to ‘3-O-Acetyl-11-keto-β-boswellic acid ameliorated aberrant metabolic landscape and inhibited autophagy in glioblastoma’ [Acta Pharm Sin B 10 (2020) 301–312]
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Wan Li, Liwen Ren, Xiangjin Zheng, Jinyi Liu, Jinhua Wang, Tengfei Ji, and Guanhua Du
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Therapeutics. Pharmacology ,RM1-950 - Published
- 2024
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3. Establishment of a mortality risk nomogram for predicting in-hospital mortality of sepsis: cohort study from a Chinese single center
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Hongsheng Wu, Shichao Jia, Biling Liao, Tengfei Ji, Jianbin Huang, Yumei Luo, Tiansheng Cao, and Keqiang Ma
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sepsis ,mortality risk ,nomogram ,cohort study ,predictive model ,Medicine (General) ,R5-920 - Abstract
ObjectiveTo establish a mortality risk nomogram for predicting in-hospital mortality of sepsis patients in the Chinese population.MethodsData were obtained from the medical records of sepsis patients enrolled at the Affiliated Huadu Hospital, Southern Medical University, between 2019 and 2021. A total of 696 sepsis patients were initially included in our research, and 582 cases were finally enrolled after screening and divided into the survival group (n = 400) and the non-survival group (n = 182) according to the incidence of mortality during hospitalization. Twenty-eight potential sepsis-related risk factors for mortality were identified. Least absolute shrinkage and selection operator (LASSO) regression was used to optimize variable selection by running cyclic coordinate descent with k-fold (tenfold in this case) cross-validation. We used binary logistic regression to build a model for predicting mortality from the variables based on LASSO regression selection. Binary logistic regression was used to establish a nomogram based on independent mortality risk factors. To validate the prediction accuracy of the nomogram, receiver operating characteristic curve (ROC) analysis, decision curve analysis (DCA) and restricted cubic spline (RCS) analysis were employed. Eventually, the Hosmer-Lemeshow test and calibration curve were used for nomogram calibration.ResultsLASSO regression identified a total of ten factors, namely, chronic heart disease (CHD), lymphocyte count (LYMP), neutrophil-lymphocyte ratio (NLR), red blood cell distribution width (RDW), C reactive protein (CRP), Procalcitonin (PCT), lactic acid, prothrombin time (PT), alanine aminotransferase (ALT), total bilirubin (Tbil), interleukin-6 (IL6), that were incorporated into the multivariable analysis. Finally, a nomogram including CHD, LYMP, NLR, RDW, lactic acid, PT, CRP, PCT, Tbil, ALT, and IL6 was established by multivariable logistic regression. The ROC curves of the nomogram in the training and validation sets were 0.9836 and 0.9502, respectively. DCA showed that the nomogram could be applied clinically if the risk threshold was between 29.52 and 99.61% in the training set and between 31.32 and 98.49% in the testing set. RCS showed that when the value of independent risk factors from the predicted model exceeded the median, the mortality hazard ratio increased sharply. The results of the Hosmer–Lemeshow test (χ2 = 0.1901, df = 2, p = 0.9091) and the calibration curves of the training and validation sets showed good agreement with the actual results, which indicated good stability of the model.ConclusionOur nomogram, including CHD, LYMP, NLR, RDW, lactic acid, PT, CRP, PCT, Tbil, ALT, and IL6, exhibits good performance for predicting mortality risk in adult sepsis patients.
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- 2024
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4. Predictive value of the neutrophil-to-lymphocyte ratio in the prognosis and risk of death for adult sepsis patients: a meta-analysis
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Hongsheng Wu, Tiansheng Cao, Tengfei Ji, Yumei Luo, Jianbin Huang, and Keqiang Ma
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adult sepsis ,NLR ,prognosis ,meta-analysis ,mortality ,Immunologic diseases. Allergy ,RC581-607 - Abstract
BackgroundThe neutrophil-to-lymphocyte ratio (NLR) is a commonly used biomarker for acute inflammation that often rises during sepsis, making it a valuable diagnostic indicator for clinical practice. However, no consensus has been reached on the prognostic value of NLR for predicting the prognosis and mortality risk in adult sepsis patients. In light of this controversy, we conducted a meta-analysis to clarify the prognostic significance of NLR in adult sepsis patients. The meta-analysis was registered in the PROSPERO database (registration number CRD42023433143).MethodsWe performed a comprehensive literature search in PubMed, Cochrane Library, Ovid, and Springer databases, using retrieval terms “sepsis” or “septic shock” and “prognosis” or “mortality” for studies published between January 1, 2000, and May 31, 2023. Children and neonates with sepsis were excluded from our research. Two independent researchers conducted the literature search and data extraction. Consensus was reached when discrepancies occurred, and in case of persistent discrepancies, the final decision was made by the research supervisor. The hazard ratio (HR) and its corresponding 95% confidence interval (95% CI) were extracted from each study included in the analysis. A random-effects model was used to synthesize all HRs and their 95% CIs. Sensitivity analysis was performed to investigate heterogeneity. Sensitivity analysis was conducted to identify studies that had a significant impact on the overall results of the meta-analysis. Subgroup analysis and meta-regression were performed to explore sources of heterogeneity. Egger’s test was also used to investigate publication bias in this meta-analysis.ResultsAfter a comprehensive literature search and screening, we included 12 studies comprising 10,811 patients for the meta-analysis. The pooled results indicated that patients with a higher NLR level were associated with a poor prognosis (Random-effects model, HR: 1.6273, 95% CI: 1.3951-1.8981). Heterogeneity testing showed significant heterogeneity (I2 = 87.2%, 95% CI: 79.5-92, p
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- 2024
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5. A nomogram for predicting in-hospital overall survival of hypertriglyceridemia-induced severe acute pancreatitis: A single center, cross-sectional study
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Hongsheng Wu, Biling Liao, Tengfei Ji, Shichao Jia, Yumei Luo, and Keqiang Ma
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Severe acute pancreatitis ,Hypertriglyceridemia ,Pretreatment nomogram ,Prognosis ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Background: Hypertriglyceridemia-induced severe acute pancreatitis (HTG-SAP) is a type of pancreatitis characterized by an abnormal elevation of plasma triglyceride. HTG-SAP has been associated with various complications and a high mortality rate. In this study, we established a nomogram for predicting the overall survival (OS) of HTG-SAP patients during hospitalization. Methods: 128 HTG-SAP cases hospitalized at the Affiliated Huadu Hospital, Southern Medical University, from 2019 to 2022 were analyzed retrospectively. A nomogram including prognostic factors correlated with OS during hospitalization was established by multivariate Cox regression analysis. We internally validated the nomogram using time-dependent (at 1-, 2-, and 3- months) survival receiver operating characteristic (SROC) and calibration curve with 500 iterations of bootstrap resampling. Time-dependent decision curve analysis (DCA) was employed to validate the clinical value of the nomogram. Results: Multivariate Cox regression indicated that serum triglyceride, red blood cell distribution width (RDW), lactic acid, and interleukin-6 (IL6) were independent prognostic factors for OS of HTG-SAP patients during hospitalization and were used to construct a nomogram. The time-dependent area under the curve (AUC) values at 1-, 2-, and 3- months were 0.946, 0.913, and 0.929, respectively, and the Concordance index (C-index) of the nomogram was 0.916 (95%CI 0.871–0.961). The time-dependent calibration curves indicated good consistency between the observed and predicted outcomes. The time-dependent DCAs also revealed that the nomogram yielded a high clinical net benefit. After stratifying the included cases into two risk groups based on the risk score obtained from the nomogram, the high-risk group exhibited a significantly inferior overall survival (OS) compared to the low-risk group (p
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- 2024
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6. Comparison of the safety profile, conversion rate and hospitalization duration between early and delayed laparoscopic cholecystectomy for acute cholecystitis: a systematic review and meta-analysis
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Hongsheng Wu, Biling Liao, Tiansheng Cao, Tengfei Ji, Jianbin Huang, Yumei Luo, and Keqiang Ma
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ELC ,DLC ,acute cholecystitis ,review ,meta-analysis ,Medicine (General) ,R5-920 - Abstract
BackgroundAlthough the past decade has witnessed unprecedented medical progress, no consensus has been reached on the optimal approach for patients with acute cholecystitis. Herein, we conducted a systematic review and meta-analysis to assess the differences in patient outcomes between Early Laparoscopic Cholecystectomy (ELC) and Delayed Laparoscopic Cholecystectomy (DLC) in the treatment of acute cholecystitis. Our protocol was registered in the PROSPERO database (registration number: CRD42023389238).ObjectivesWe sought to investigate the differences in efficacy, safety, and potential benefits between ELC and DLC in acute cholecystitis patients by conducting a systematic review and meta-analysis.MethodsThe online databases PubMed, Springer, and the Cochrane Library were searched for randomized controlled trials (RCTs) and retrospective studies published between Jan 1, 1999 and Jan 1, 2022.Results21 RCTs and 13 retrospective studies with a total of 7,601 cases were included in this research. After a fixed-effects model was applied, the pooled analysis showed that DLC was associated with a significantly high conversion rate (OR: 0.6247; 95%CI: 0.5115–0.7630; z = −4.61, p
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- 2023
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7. Establishment of a 7-gene prognostic signature based on oxidative stress genes for predicting chemotherapy resistance in pancreatic cancer
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Shengmin Zhang, Jianrong Yang, Hongsheng Wu, Tiansheng Cao, and Tengfei Ji
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pancreatic cancer ,oxidative stress ,methylation ,molecular subtypes ,risk score ,small molecule chemotherapeutic drugs ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Background: Oxidative stress is involved in regulating various biological processes in human cancers. However, the effect of oxidative stress on pancreatic adenocarcinoma (PAAD) remained unclear.Methods: Pancreatic cancer expression profiles from TCGA were downloaded. Consensus ClusterPlus helped classify molecular subtypes based on PAAD prognosis-associated oxidative stress genes. Limma package filtered differentially expressed genes (DEGs) between subtypes. A multi-gene risk model was developed using Lease absolute shrinkage and selection operator (Lasso)-Cox analysis. A nomogram was built based on risk score and distinct clinical features.Results: Consistent clustering identified 3 stable molecular subtypes (C1, C2, C3) based on oxidative stress-associated genes. Particularly, C3 had the optimal prognosis with the greatest mutation frequency, activate cell cycle pathway in an immunosuppressed status. Lasso and univariate cox regression analysis selected 7 oxidative stress phenotype-associated key genes, based on which we constructed a robust prognostic risk model independent of clinicopathological features with stable predictive performance in independent datasets. High-risk group was found to be more sensitive to small molecule chemotherapeutic drugs including Gemcitabine, Cisplatin, Erlotinib and Dasatinib. The 6 of 7 genes expressions were significantly associated with methylation. Survival prediction and prognostic model was further improved through a decision tree model by combining clinicopathological features with RiskScore.Conclusion: The risk model containing seven oxidative stress-related genes may have a greater potential to assist clinical treatment decision-making and prognosis determination.
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- 2023
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8. Bioinformatics-based construction of prognosis-related methylation prediction model for pancreatic cancer patients and its application value
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Tiansheng Cao, Hongsheng Wu, and Tengfei Ji
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pancreatic cancer ,methylation ,prognosis ,prediction model ,drugs ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Objective: Pancreatic adenocarcinoma (PAAD) is a highly malignant gastrointestinal tumor with almost similar morbidity and mortality. In this study, based on bioinformatics, we investigated the role of gene methylation in PAAD, evaluated relevant factors affecting patient prognosis, screened potential anti-cancer small molecule drugs, and constructed a prediction model to assess the prognosis of PAAD.Methods: Clinical and genomic data of PAAD were collected from the Tumor Genome Atlas Project (TCGA) database and gene expression profiles were obtained from the GTEX database. Analysis of differentially methylated genes (DMGs) and significantly differentially expressed genes (DEGs) was performed on tumorous samples with KRAS wild-type and normal samples using the “limma” package and combined analysis. We selected factors significantly associated with survival from the significantly differentially methylated and expressed genes (DMEGs), and their fitting into a relatively streamlined prognostic model was validated separately from the internal training and test sets and the external ICGC database to show the robustness of the model.Results: In the TCGA database, 2,630 DMGs were identified, with the largest gap between DMGs in the gene body and TSS200 region. 318 DEGs were screened, and the enrichment analysis of DMGs and DEGs was taken to intersect DMEGs, showing that the DMEGs were mainly related to Olfactory transduction, natural killer cell mediated cytotoxicity pathway, and Cytokine -cytokine receptor interaction. DMEGs were able to distinguish well between PAAD and paraneoplastic tissues. Through techniques such as drug database and molecular docking, we screened a total of 10 potential oncogenic small molecule compounds, among which felbamate was the most likely target drug for PAAD. We constructed a risk model through combining three DMEGs (S100P, LY6D, and WFDC13) with clinical factors significantly associated with prognosis, and confirmed the model robustness using external and internal validation.Conclusion: The classification model based on DMEGs was able to accurately separate normal samples from tumor samples and find potential anti-PAAD drugs by performing gene-drug interactions on DrugBank.
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- 2023
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9. High Expression of Ten Eleven Translocation 1 Is Associated with Poor Prognosis in Hepatocellular Carcinoma
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Haopeng Wen, Tengfei Ji, Liteng Lin, Nan Cheng, Kangshun Zhu, and Liangqi Cao
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Pathology ,RB1-214 - Abstract
Background. DNA methylation patterns have been found to be distinct between tumor and normal patients. However, the effect of DNA demethylation enzymes, ten eleven translocation (TET) proteins, has not been comprehensively characterized in liver cancer. In this research, we sought to unravel the linkage of TET proteins with prognosis, immune characteristics and biological pathways in hepatocellular carcinoma (HCC). Materials and Methods. Four independent datasets with gene expression data and clinical data of HCC samples were downloaded from public databases. CIBERSORT, single sample Gene Set Enrichment Analysis (ssGSEA), MCP-counter, and TIMER were implemented to evaluate immune cell infiltration. limma was employed to screen differentially expressed genes (DEGs) between two groups. The demethylation-related risk model was established by using univariate Cox regression analysis, the least absolute shrinkage and selection operator (LASSO), and stepwise Akaike information criterion (stepAIC). Results. TET1 was significantly higher expressed in tumor samples than that in normal samples. HCC patients with advanced stages (III+IV) and grades (G3+G4) had higher TET1 expression compared to early stages (I+II) and grades (G1+G2). HCC samples with high TET1 expression had worse prognosis than that with low expression. High and low TET1 expression groups had distinct immune cell infiltration and response to immunotherapy and chemotherapy. We identified 90 DEGs related to DNA demethylation in high vs. low TET1 expression groups. Furthermore, we established a risk model based on 90 DEGs containing seven key prognostic genes (SERPINH1, CDC20, HACD2, SPHK1, UGT2B15, SLC1A5, and CYP2C9) with effectiveness and robustness in predicting HCC prognosis. Conclusions. Our study suggested TET1 as a potential indicator in HCC progression. TET1 was closely involved in immune infiltration and activation of oncogenic pathways. The DNA demethylation-related risk model was potential to be applied for predicting HCC prognosis in clinics.
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- 2023
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10. Bioinspired desaturation of alcohols enabled by photoredox proton-coupled electron transfer and cobalt dual catalysis
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Long Huang, Tengfei Ji, Chen Zhu, Huifeng Yue, Nursaya Zhumabay, and Magnus Rueping
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Science - Abstract
Dehydrogenative reactions can provide alkenes, which are among the most useful handles for synthetic organic chemists. Here the authors integrated photoredox proton-coupled electron transfer and cobaloxime chemistry for the acceptorless dehydrogenation of alkyl alcohols.
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- 2022
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11. Sinomenine ester derivative inhibits glioblastoma by inducing mitochondria-dependent apoptosis and autophagy by PI3K/AKT/mTOR and AMPK/mTOR pathway
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Xiangjin Zheng, Wan Li, Huanli Xu, Jinyi Liu, Liwen Ren, Yihui Yang, Sha Li, Jinhua Wang, Tengfei Ji, and Guanhua Du
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SW33 ,GBM ,G2/M phase ,Apoptosis ,Autophagy ,mTOR ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Glioblastoma multiforme (GBM) in the central nervous system is the most lethal advanced glioma and currently there is no effective treatment for it. Studies of sinomenine, an alkaloid from the Chinese medicinal plant, Sinomenium acutum, showed that it had inhibitory effects on several kinds of cancer. Here, we synthesized a sinomenine derivative, sino-wcj-33 (SW33), tested it for antitumor activity on GBM and explored the underlying mechanism. SW33 significantly inhibited proliferation and colony formation of GBM and reduced migration and invasion of U87 and U251 cells. It also arrested the cell cycle at G2/M phase and induced mitochondria-dependent apoptosis. Differential gene enrichment analysis and pathway validation showed that SW33 exerted anti-GBM effects by regulating PI3K/AKT and AMPK signaling pathways and significantly suppressed tumorigenicity with no obvious adverse effects on the body. SW33 also induced autophagy through the PI3K/AKT/mTOR and AMPK/mTOR pathways. Thus, SW33 appears to be a promising drug for treating GBM effectively and safely.
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- 2021
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12. Diagnostic value of RDW for the prediction of mortality in adult sepsis patients: A systematic review and meta-analysis
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Hongsheng Wu, Biling Liao, Tiansheng Cao, Tengfei Ji, Jianbin Huang, and Keqiang Ma
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red blood cell distribution width ,sepsis ,diagnostic value ,mortality ,prognosis ,meta-analysis ,Immunologic diseases. Allergy ,RC581-607 - Abstract
BackgroundRed blood cell distribution width (RDW) is a common biomarker of bacterial infections, and it can be easily obtained from a routine blood test. We investigate the diagnostic value of RDW for the prediction of mortality in adult sepsis patients through a review and meta-analysis. We registered this review in PROSPERO (Registration Number: CRD42022357712), and the details of the registration are included in Appendix 1.MethodsWe searched PubMed, Cochrane Library, Springer, and Embase between Jan. 1, 2000, and May 30, 2022, for primary studies about this research. We collected articles that investigated RDW for varying degrees of sepsis patients—those who suffered from sepsis, severe sepsis, or sepsis shock. Studies of healthy people and sepsis of children and neonates were excluded from our research. The definition of study characteristics and data extraction were finished by two independent researchers and discrepancies resolved by consensus. The combined sensitivities and specificities were calculated by meta-analysis using STATA14.0. The sensitivity of the included studies was analyzed by excluding studies that had potential heterogeneity. A summary operating characteristic curve was made to evaluate the diagnostic value for the prediction of mortality in adult sepsis patients. The Fagan test was used to explore likelihood ratios and posttest probabilities. Finally, we investigated the source of heterogeneity using meta-regression.ResultsTwenty-four studies, including 40,763 cases altogether, were included in this analysis. Bivariate analysis indicated a combined sensitivity of 0.81 (95% CI 0.73–0.86) and specificity of 0.65 (95% CI 0.54–0.75). The area under the summary receiver operating characteristic curve was 0.81 (95% CI 0.77–0.84). Substantial heterogeneity resided in the studies (I2 =96.68, 95% CI 95.95–97.4). Meta-regression showed that the reference description, prospective design, and blinded interpretation of the included studies could be responsible for the heterogeneity.ConclusionsRWD is an available and valuable biomarker for prediction of mortality in adult sepsis patients.Systematic review registrationhttps://www.crd.york.ac.uk/PROSPERO/, identifier CRD42022357712.
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- 2022
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13. Correction: 3-O-acetyl-11-keto-β-boswellic acid exerts anti-tumor effects in glioblastoma by arresting cell cycle at G2/M phase
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Wan Li, Jinyi Liu, Weiqi Fu, Xiangjin Zheng, Liwen Ren, Shiwei Liu, Jinhua Wang, Tengfei Ji, and Guanhua Du
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Published
- 2022
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14. A Substance P (SP)/Neurokinin-1 Receptor Axis Promotes Perineural Invasion of Pancreatic Cancer and Is Affected by lncRNA LOC389641
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Tengfei Ji, Keqiang Ma, Hongsheng Wu, and Tiansheng Cao
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Immunologic diseases. Allergy ,RC581-607 - Abstract
Perineural invasion (PNI) is considered to be a main reason for the poor prognosis of pancreatic cancer. In the present study, we analyzed the roles of substance P (SP)/neurokinin-1 receptor (NK-1R) and lncRNA LOC389641 in pancreatic cancer PNI. Pancreatic cancer cell lines BxPC-3 and MIAPaCa-2 were cocultured with SH-SY5Y cells and then stimulated with SP to simulate the in vivo influence of ganglia on pancreatic cancer. The BxPC-3 and MIAPaCa-2 cells were transfected with a neurokinin-1 receptor (NK-1R) overexpression vector, NK-1R silencing vector, LOC389641 overexpression vector, or LOC389641 silencing vector, respectively. The proliferative abilities of BxPC-3 and MIAPaCa-2 cells were assessed using the cell counting kit-8 and 5-ethynyl-2′-deoxyuridine (EdU) assays. Wound-healing and Transwell assays were performed to determine the migration and invasion abilities of the cells. When SP was added to the coculture system, it positively regulated cancer cell proliferation, migration, and PNI and significantly activated the NK-1R/Akt/NF-κB signaling pathway. Incubation with 100 nmol/L SP for 24 h was selected as the optimal condition for treatment. The activated NK-1R positively regulated the proliferation, migration, and invasion of pancreatic cancer cells. However, the levels of lncRNA LOC389641 and tumor necrosis factor receptor SF10A (TNFRSF10A) mRNA in BxPC-3 and MIAPaCa-2 cells were not affected by SP treatment. Overexpression or silencing of LOC389641 changed the effect of SP stimulation on pancreatic cancer PNI. When taken together, these results revealed that SP/NK-1R and LOC389641 promoted the progression of pancreatic cancer PNI. Moreover, we found that pancreatic cancer PNI promoted by the SP/NK-1R axis could be blocked by the TNFRSF10A/NF-κB pathway mediated by LOC389641.
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- 2022
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15. 3-O-Acetyl-11-keto-β-boswellic acid ameliorated aberrant metabolic landscape and inhibited autophagy in glioblastoma
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Wan Li, Liwen Ren, Xiangjin Zheng, Jinyi Liu, Jinhua Wang, Tengfei Ji, and Guanhua Du
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Therapeutics. Pharmacology ,RM1-950 - Abstract
Glioblastoma is the most common and aggressive primary tumor in the central nervous system, accounting for 12%–15% of all brain tumors. 3-O-Acetyl-11-keto-β-boswellic acid (AKBA), one of the most active ingredients of gum resin from Boswellia carteri Birdw., was reported to inhibit the growth of glioblastoma cells and subcutaneous glioblastoma. However, whether AKBA has antitumor effects on orthotopic glioblastoma and the underlying mechanisms are still unclear. An orthotopic mouse model was used to evaluate the anti-glioblastoma effects of AKBA. The effects of AKBA on tumor growth were evaluated using MRI. The effects on the alteration of metabolic landscape were detected by MALDI-MSI. The underlying mechanisms of autophagy reducing by AKBA treatment were determined by immunoblotting and immunofluorescence, respectively. Transmission electron microscope was used to check morphology of cells treated by AKBA. Our results showed that AKBA (100 mg/kg) significantly inhibited the growth of orthotopic U87-MG gliomas. Results from MALDI-MSI showed that AKBA improved the metabolic profile of mice with glioblastoma, while immunoblot assays revealed that AKBA suppressed the expression of ATG5, p62, LC3B, p-ERK/ERK, and P53, and increased the ratio of p-mTOR/mTOR. Taken together, these results suggested that the antitumor effects of AKBA were related to the normalization of aberrant metabolism in the glioblastoma and the inhibition of autophagy. AKBA could be a promising chemotherapy drug for glioblastoma. KEY WORDS: Glioblastoma, AKBA, MALDI-MSI, Phospholipids, Autophagy
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- 2020
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16. Hyperacmosin R, a New Decarbonyl Prenylphloroglucinol with Unusual Spiroketal Subunit from Hypericum acmosepalum
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Yonghui Ma, Xiaoyu Liu, Bo Liu, Pingping Li, Xinyue Suo, Tingting Zhu, Tengfei Ji, Jin Li, and Xiaoxiu Li
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Hypericum acmosepalum ,PPAPs ,5,8-spiroketal ,hepatoprotective activity ,Organic chemistry ,QD241-441 - Abstract
Two previously undescribed polycyclic polyprenylated acylphloroglucinols, hyperacmosins R-S (1–2), were obtained from the aerial parts of Hypericum acmosepalum. Their structures were elucidated by extensive spectroscopic analysis and electronic circular dichroism calculation (ECD). Compound 1 featured an unprecedented 5,8-spiroketal subunit as well as the loss of C-2′ carbonyl in the phloroglucinol ring. In addition, compounds 1 and 4 showed weak hepatoprotective activity against paracetamol-induced HepG2 cell damage at 10 μm. The plausible biosynthetic pathway of 1 was proposed via a retro-Clasisen reaction and decarboxylation.
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- 2022
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17. Correction: Wang et al. Polycyclic Polyprenylated Acylphloroglucinol Derivatives from Hypericum acmosepalum. Molecules 2019, 24, 50
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Jiao Wang, Mengjiao Shi, Jiajia Wang, Jin Li, and Tengfei Ji
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n/a ,Organic chemistry ,QD241-441 - Abstract
The authors wish to make the following correction to their paper [...]
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- 2022
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18. 3-O-acetyl-11-keto-β-boswellic acid exerts anti-tumor effects in glioblastoma by arresting cell cycle at G2/M phase
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Wan Li, Jinyi Liu, Weiqi Fu, Xiangjin Zheng, Liwen Ren, Shiwei Liu, Jinhua Wang, Tengfei Ji, and Guanhua Du
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Glioblastoma ,AKBA ,Apoptosis ,Cell cycle ,p21/FOXM1/cyclin B1 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Glioblastoma (GBM) is the most common, malignant, and lethal primary brain tumor in adults accounting for about 50% of all gliomas. Up to now, the chemotherapy approaches for GBM were limited. 3-O-acetyl-11-keto-β-boswellic acid (AKBA), the major active ingredient of the gum resin from Boswellia serrata and Boswellia carteri Birdw., was reported to inhibit the growth of many types of cancer cells; however, the underlying mechanism of its anticancer effects are still unclear. Methods The effects of AKBA on cell viability and its cytotoxicity were determined using CCK8 and LDH kits respectively. The EdU-DNA synthesis assay was used to evaluate inhibition of cell proliferation by AKBA. The role of AKBA in glioblastoma cell functions such as migration/invasion, and colony formation was evaluated using transwell chambers and soft agar, respectively. Flow cytometry and western blotting were used to detect AKBA-induced apoptosis. Potential mechanisms of AKBA action were explored by RNA sequencing and the identified hub genes were validated by real-time quantitative PCR and western blotting. Finally, the in vivo anti-tumor activity of AKBA was evaluated against a human glioblastoma cell line, U87-MG, in a xenograft mouse model. Results AKBA inhibited cell proliferation, caused the release of LDH, decreased DNA synthesis, and inhibited the migration, invasion, and colony formation of U251 and U87-MG human glioblastoma cell lines. AKBA increased apoptosis as well as the activity of caspase 3/7 and the protein expression of cleaved-caspase 3 and cleaved PARP, while decreasing mitochondrial membrane potential. RNA-sequencing analyses showed that AKBA suppressed the expression of pRB, FOXM1, Aurora A, PLK1, CDC25C, p-CDK1, cyclinB1, Aurora B, and TOP2A while increasing the expression of p21 and GADD45A. These findings were validated by qRT-PCR and western blotting. The data are consistent with a mechanism in which AKBA arrested the cell cycle in glioblastoma cells at the G2/M phase by regulating the p21/FOXM1/cyclin B1 pathway, inhibited mitosis by downregulating the Aurora B/TOP2A pathway, and induced mitochondrial-dependent apoptosis. Oral administration of AKBA (100 mg/kg) significantly suppressed the tumorigenicity of U87-MG cells in a xenograft mouse model. Conclusions Taken together, these results suggest that AKBA (molecular weight, 512.7 Da) might be a promising chemotherapy drug in the treatment of GBM.
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- 2018
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19. Four New Triterpenoids from Callicarpa kwangtungensis
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Guo-Ping Zhou, Yan Yu, Ming-Ming Yuan, Tengfei Ji, Hui-Zheng Fu, and Rui-Jian Zhong
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Callicarpa kwangtungensis ,Verbenaceae ,triterpenoids ,NMR ,MS ,Organic chemistry ,QD241-441 - Abstract
Four new triterpenoids which were identifed as 2α,3β,6β,19α-tetrahydroxy- oleanolic acid 28-O-β-D-glucopyranoside (1), 2-O-β-D-glucopyranosyloxy-3α,19α-di-hydroxyoleanolic acid (2), 2-O-β-D-glucopyranosyloxy-3α,19α-dihydroxyursolic acid (3), 2α,3α,6β,19α-tetrahydroxyursolic acid 28-O-β-D-glucopyranoside (4), were isolated from the aerial parts of Callicarpa kwangtungensis together with three known triterpenoids identified as 2α,3β,21β-trihydroxyursolic acid 28-O-β-D-glucopyranoside (5), 2α,3α,19α,23-tetrahydroxyoleanolic acid 28-O-β-D-glucopyranoside (6), 2α,3α,19α,23-tetrahydroxyursolic acid 28-O-β-D-glucopyranoside (7). Their structures were elucidated by the combination of mass spectrometry (MS), one and two-dimensional NMR experiments.
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- 2015
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20. Two New Bidesmoside Triterpenoid Saponins from the Seeds of Momordica charantia L.
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Lin Ma, Ai-Hua Yu, Li-Li Sun, Wan Gao, Meng-Meng Zhang, Ya-Lun Su, Hua Liu, and Tengfei Ji
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Momordica charantia L. ,bidesmoside ,triterpenoid saponin ,NMR ,MS ,Organic chemistry ,QD241-441 - Abstract
Two new bidesmoside triterpenoid saponins which were identifed as 28-O-β-D-xylopyranosyl(1→3)-β-D-xylopyranosyl(1→4)-α-L-rhamnopyranosyl(1→2)-[α-L-rhamno-pyranosyl(1→3)]-β-D-fucopyranosyl gypsogenin 3-O-β-D-glucopyranosyl (1→2)-β-D-glucopyranosiduronic acid (C1) and 28-O-β-D-xylopyranosyl(1→4)-α-L-rhamnopyranosyl (1→2)-[α-L-rhamnopyranosyl(1→3)]-β-D-fucopyranosyl gypsogenin 3-O-β-D-gluco-pyranosyl(1→2)-β-D-glucopyranosiduronic acid (C2) were isolated together with two known compounds from the seeds of Momordica charantia L. Their structures were elucidated by the combination of mass spectrometry (MS), one and two-dimensional NMR experiments and chemical reactions.
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- 2014
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21. Polycyclic Polyprenylated Acylphloroglucinol Derivatives from Hypericum acmosepalum
- Author
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Jiao Wang, Mengjiao Shi, Jiajia Wang, Jin Li, and Tengfei Ji
- Subjects
Hypericum acmosepalum ,polycyclic polyprenylated acylphloroglucinols (PPAPs) ,neuroprotective effect ,Organic chemistry ,QD241-441 - Abstract
Hypericum acmosepalum belongs to the Hypericum genus of the Guttiferae family. The characteristic components in Hypericum are mainly a series of polycyclic polyprenylated acylphloroglucinols (PPAPs), flavonoids, and xanthones. Among them, the PPAPs have received much attention due to their novel structures and diverse pharmacological activities and have become hot spots in organic chemistry and medicinal chemistry. However, there are few reports about the chemical constituents of Hypericum acmosepalum at present, especially the PPAPs. This research is dedicated to the study of the air-dried aerial parts of Hypericum acmosepalum, which were extracted with 95% EtOH under reflux, then suspended and successively partitioned with petroleum ether and ethyl acetate. Five PPAP derivatives were obtained using various chromatographic techniques, and their structures were determined by NMR spectroscopic data, including two new phloroglucinol derivatives, hyperacmosin A (1) and hyperacmosin B (2). Those compounds were evaluated for their neuroprotective effect using two models.
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- 2018
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22. Hydrolyzable tannins from Balanophora polyandra
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Yangai Wang, Jianbo Yang, Aiguo Wang, Jie Ma, Jin Tian, Tengfei Ji, and Yalun Su
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Balanophora polyandra ,Hydrolyzable tannin ,1,4-di-O-galloyl-2-O-[(E)-p-coumaroyl]-β-D-glucopyranose ,Therapeutics. Pharmacology ,RM1-950 - Abstract
This study reports an investigation of the chemical constituents of Balanophora polyandra Griff. Fifteen compounds were isolated by column chromatography on silica gel, Toyo-pearl HW-40C, Sephadex LH-20 and by HPLC. Their structures were elucidated as 1,4-di-O-galloyl-2-O-[(E)-p-coumaroyl]-β-D-glucopyranose (1), 1-O-galloyl-β-D-pyranglucose (2), 1-p-coumaryl-β-D-pyranglucose (3), 1-O-(E)-caffeoyl-β-D-pyranglucose (4), 1,3-di-O-galloyl-β-D-pyranglucose (5), 1,6-di-O-galloyl-β-D-pyranglucose (6), 1-O-(E)-caffeoyl-4-O-galloyl-β-D-pyranglucose (7), 1-O-(E)-caffeoyl-6-O-galloyl-β-D-pyranglucose (8), 1-O-(E)-caffeoyl-4,6-di-O-galloyl-β-D-pyranglucose (9), 1-O-(E)-caffeoyl-4,6-(S)-HHDP-β-D-pyranglucose (10), 1,2,3,6-tetra-O-galloyl-β-D-pyranglucose (11), 4,6-(S)-hexahydroxydiphenoyl-(α/β)-D-glucose (12), 1-O-(E)-caffeoyl-4,6-[1′,1″-(3′,3″,4′,4″-tetrahydroxydibenzofurandicarboxyl)]-β-D-glucopyranose (13), flavogallonic acid (14), and phloretin-4′-O-β-D-glucoside (15) on the basis of spectral analysis. Compound 1 was a new hydrolyzable tannin, 9 was obtained from this genus for the first time, and compounds 5, 6 and 11–14 were isolated from this plant for the first time.
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- 2013
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23. Immobilization of Laccase for Oxidative Coupling of Trans-Resveratrol and Its Derivatives
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Tiexin Cheng, Zhi Wang, Tengfei Ji, Lei Wang, Ge Chen, Hong Zhang, Jiaxin Wang, and Erna Xun
- Subjects
laccase ,SBA-15 ,immobilization ,oxidative coupling ,resveratrol ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Trametes villosa Laccase (TVL) was immobilized through physical adsorption on SBA-15 mesoporous silica and the immobilized TVL was used in the oxidative coupling of trans-resveratrol. Higher loading and activity of the immobilized enzyme on SBA-15 were obtained when compared with the free enzyme. The effects of reaction conditions, such as buffer type, pH, temperature and substrate concentration were investigated, and the optimum conditions were screened and resulted in enzyme activity of up to 10.3 μmol/g·h. Furthermore, the oxidative couplings of the derivatives of trans-resveratrol were also catalyzed by immobilized TVL. The immobilized TVL was recyclable and could maintain 78% of its initial activity after reusing it four times.
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- 2012
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24. Hepatoprotective Effects of Nicotiflorin from Nymphaea candida against Concanavalin A-Induced and D-Galactosamine-Induced Liver Injury in Mice
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Jun Zhao, Shilei Zhang, Shuping You, Tao Liu, Fang Xu, Tengfei Ji, and Zhengyi Gu
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nicotiflorin ,Nymphaea candida ,hepatoprotective ,Concanavalin A ,D-galactosamine ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Nymphaea candida was used to treat hepatitis in Ugyhur medicine, and nicotiflorin (kaempferol 3-O-β-rutinoside) is the main characteristic component in this plant [...]
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- 2017
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25. Vibration Analysis of Heating-Used Steam Extraction Pipelines in Nuclear Power Plant
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Yanhui, Wu, Yaping, Zhuang, Tengfei, Ji, and Liu, Chengmin, editor
- Published
- 2023
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26. A Triangle Framework among Subgraph Isomorphism, Pharmacophore and Structure-function Relationship.
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Mengjiao Guo, Hui Zheng, Tengfei Ji, Hu Fa, and Jing He 0004
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- 2022
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27. A new Technique in Protein Structure Quantitative Identification.
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Mengjiao Guo, Haobin Cao, Jing He, and Tengfei Ji
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- 2022
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28. Nickel-Catalyzed Reductive Cross-Couplings: New Opportunities for Carbon–Carbon Bond Formations through Photochemistry and Electrochemistry
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Tengfei Ji, Kun-Quan Chen, Liang Yi, Xiang-Yu Chen, and Magnus Rueping
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chemistry.chemical_classification ,Nickel ,Chemistry ,Carbon–carbon bond ,chemistry.chemical_element ,General Chemistry ,Bond formation ,Electrochemistry ,Photochemistry ,Catalysis - Abstract
Metal-catalyzed cross-electrophile couplings have become a valuable tool for carbon–carbon bond formation. This minireview provides a comprehensive overview of the recent developments in the topica...
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- 2022
29. Incremental Local Evolutionary Outlier Detection for Dynamic Social Networks.
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Tengfei Ji, Dongqing Yang, and Jun Gao 0003
- Published
- 2013
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30. A Scalable Algorithm for Detecting Community Outliers in Social Networks.
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Tengfei Ji, Jun Gao 0003, and Dongqing Yang
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- 2012
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31. Preparative isolation of antioxidative gallic acid derivatives from Saxifraga tangutica using a class separation method based on medium‐pressure liquid chromatography and reversed‐phase liquid chromatography
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Gang Li, Qi Wang, Chen Yuan, Jun Dang, Qilan Wang, and Tengfei Ji
- Subjects
Chromatography, Reverse-Phase ,Chromatography ,Saxifraga tangutica ,Biphenyl Compounds ,Saxifragaceae ,Filtration and Separation ,Fraction (chemistry) ,Reversed-phase chromatography ,Antioxidants ,Protocatechuic acid ,Analytical Chemistry ,Separation process ,chemistry.chemical_compound ,Picrates ,chemistry ,Gallic Acid ,Separation method ,Gallic acid ,Methanol ,Chromatography, High Pressure Liquid - Abstract
Saxifraga tangutica is widely used as a medicinal herb to treat hepatic diseases. Here, we developed a class separation method to separate gallic acid derivatives 1,1-diphenyl-2-picrylhydrazyl inhibitors from the methanol extract of Saxifraga tangutica. Firstly, an MCI GEL CHP20P medium-pressure liquid chromatography was used to pretreat the crude extract from Saxifraga tangutica (500 g) and the target sample (fraction Fr1, 1.7 g) was obtained. Then, an online reversed-phase liquid chromatography-1,1-diphenyl-2-picrylhydrazyl assay was employed for recognizing potential 1,1-diphenyl-2-picrylhydrazyl inhibitors and six 1,1-diphenyl-2-picrylhydrazyl inhibitors fractions were recognized from fraction Fr1. Subsequently, the six 1,1-diphenyl-2-picrylhydrazyl inhibitors fractions were isolated via a ReproSil-Pur C18 AQ preparative column. During the separation process, the hydrophilic liquid chromatography was used to enrich the target compounds (Fr1-3-1-1 and Fr1-3-1-2) from the fraction Fr1-3, which were hardly isolated only by one step reversed-phase liquid chromatography. Finally, six gallic acid derivatives were obtained and identified as gallic acid (Fr1-1-1), gallic acid 3-O-β-D-glucoside (Fr1-1-2), protocatechuic acid (Fr1-2), 4-O-galloyl-(-)-shikimic acid (Fr1-3-1-1), 5-O-galloyl-(-)-shikimic acid (Fr1-3-1-2), and 3-O-galloyl-shikimic acid (Fr1-4), respectively. Thus, the present study indicated that this method was highly efficient for the preparative separation of gallic acid derivatives 1,1-diphenyl-2-picrylhydrazyl inhibitors from natural products.
- Published
- 2021
32. A Fuzzy K-modes-based Algorithm for Soft Subspace Clustering.
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Tengfei Ji, Xiaoyuan Bao, Yue Wang 0014, and Dongqing Yang
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- 2011
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33. FXProj - A Fuzzy XML Documents Projected Clustering Based on Structure and Content.
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Tengfei Ji, Xiaoyuan Bao, and Dongqing Yang
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- 2011
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34. Rigid-Membrane Method for Determining Stress Distribution of Membrane Structure Based on Laser Scanner System
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Xiangyu Zhang, Shasha Wang, Jinghai Gong, Guozhi Qiu, and Tengfei Ji
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Multidisciplinary - Published
- 2022
35. Constructing a Novel Prognostic Signature Based on TGF
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Tengfei, Ji, Hongsheng, Wu, and Keqiang, Ma
- Abstract
Pancreatic adenocarcinoma (PAAD) shows significantly high mortality. Transforming growth factor-beta (TGF-Datasets were grouped into test and training sets. Univariate Cox regression analysis and least absolute shrinkage and selection operator (LASSO) were applied and introduced for identifying prognostic genes associated with TGF-Based on TGF-Notably, the prognostic signature can act as a predictor of prognosis, but as a biomarker for optimizing personalized therapies in clinical practice.
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- 2022
36. PADI1 contributes to EMT in PAAD by activating the ERK1/2-p38 signaling pathway
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Keqiang Ma, Tengfei Ji, Liang Chen, and Tiansheng Cao
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Gene knockdown ,Oncogene ,medicine.diagnostic_test ,business.industry ,Gastroenterology ,Cell migration ,medicine.disease_cause ,medicine.disease ,Metastasis ,Oncology ,Western blot ,RNA interference ,medicine ,Cancer research ,Original Article ,Signal transduction ,Carcinogenesis ,business - Abstract
Background Peptidylarginine deiminase 1 (PADI1) has been reported to promote tumorigenesis in breast cancer. However, the functional role of PADI1 in pancreatic ductal adenocarcinoma (PAAD) has remained elusive until now. Methods The expression pattern of PADI1 in PAAD tissues and normal tissues was analyzed using The Cancer Genome Atlas (TCGA) dataset. A Kaplan-Meier curve analysis was performed to evaluate the prognostic value of PADI1 in PAAD patients. PADI1 was knocked down in CFPAN-1 and HPAC cells, and overexpressed in PANC-1 and Bxpc-3 cells by RNA interference. A wound-healing assay was performed to analyze relative cell migration distance. Cell migration and invasion were assessed by a Transwell assay. Related protein expression levels were measured by western blot and immunofluorescence. Results The bioinformatics analysis showed that PADI1 was overexpressed in PAAD tissues and associated with a poor survival prognosis. The knockdown of PADI1 suppressed cell migration and invasion, and activated the ERK1/2-p38 signaling pathway in CFPAN-1 and HPAC cells. The overexpression of PADI1 produced the opposite results in PANC-1 and Bxpc-3 cells. Additionally, treatment with an MEK1/2 inhibitor significantly attenuated the effects of PADI1 knockdown on cell migration, invasion, the epithelial-mesenchymal transition (EMT) process, and p-ERK1/2 and p38 expression in CFPAN-1 and HPAC cells. Conclusions Our data suggested that PADI1 may function as an oncogene in regulating metastasis in vitro in PAAD.
- Published
- 2021
37. A sensitive LC-MS method for the determination of Sinomenine derivative SWX and its application to pharmacokinetic research in rats
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Binbin Ge, Xuejian Wang, Wan Li, Sha Li, Yanmei Du, Tengfei Ji, Guanhua Du, Chunyan Fang, and Jinhua Wang
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Acetonitriles ,Tandem Mass Spectrometry ,Clinical Biochemistry ,Solvents ,Animals ,Reproducibility of Results ,Cell Biology ,General Medicine ,Biochemistry ,Chromatography, High Pressure Liquid ,Analytical Chemistry ,Rats ,Chromatography, Liquid - Abstract
The goal of this work was to develop a sensitive and accurate method based on high performance liquid chromatography with tandem mass spectrometry (LC-MS) detection for determining the concentration of the Sinomenine derivative SWX in plasma and tissue of rat. Chromatographic separation was achieved on an Agilent SB-C18 (2.1*50 mm, 2.7 µm) column. The mobile phase consisted of acetonitrile (solvent A) and 0.2 % formic acid in water (solvent B) with gradient elution as follows: 0-3 min, 50-90 % A, 3 min-3.01 min, 90-50 % A, 90 % A in 3.01 min-10 min. The flow rate was set to 0.3 mL/min. The column temperature was 40 °C, and the sample injection volume was 10 µL. The calibration curve had good linearity in the range of 10 ng/mL to 600 ng/mL. Biological samples were prepared by protein precipitation with acetonitrile. The area under the curve (AUC) and the peak drug concentration (C
- Published
- 2022
38. Two new polycyclic polyprenylated acylphloroglucinols derivatives from Hypericum acmosepalum
- Author
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Hao-Ran Sun, Jia-Jia Wang, Bo Zhen, Hua Sun, Xue Wang, Qiang Guifen, Bo Liu, Meng-Jiao Shi, Xin-Yue Suo, Tengfei Ji, Huilan Yue, Jun Dang, and Yanduo Tao
- Subjects
Pharmacology ,Traditional medicine ,010405 organic chemistry ,Organic Chemistry ,Pharmaceutical Science ,General Medicine ,Biology ,Hypericum acmosepalum ,biology.organism_classification ,01 natural sciences ,0104 chemical sciences ,Analytical Chemistry ,010404 medicinal & biomolecular chemistry ,Complementary and alternative medicine ,Genus ,Drug Discovery ,Molecular Medicine ,Hypericum - Abstract
Polycyclic polyprenylated acylphloroglucinols (PPAPs) were mainly obtained from the plants of Hypericum genus of Guttiferae family, and possessed intriguing chemical structures and appealing biolog...
- Published
- 2021
39. Preparative separation of 1,1-diphenyl-2-picrylhydrazyl inhibitors originating from Saxifraga sinomontana employing medium-pressure liquid chromatography in combination with reversed-phase liquid chromatography
- Author
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Jun Dang, Jianbin Ma, Yangzom Dawa, Chuang Liu, Tengfei Ji, and Qilan Wang
- Subjects
General Chemical Engineering ,General Chemistry - Abstract
Preparative separation of 1,1-diphenyl-2-picrylhydrazyl inhibitors originating from Saxifraga sinomontana employing medium-pressure liquid chromatography in combination with reversed-phase liquid chromatography.
- Published
- 2021
40. Comparative Analysis of Early Clinical Features and Complications of Different Types of Acute Pancreatitis
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Hongsheng Wu, Keqiang Ma, Biling Liao, Tengfei Ji, Shengmin Zhang, and Tiansheng Cao
- Subjects
Aging ,Article Subject ,Pancreatitis ,Acute Disease ,Humans ,Cell Biology ,General Medicine ,Biochemistry ,Severity of Illness Index ,APACHE ,Retrospective Studies - Abstract
Background. Acute pancreatitis (AP) is a common surgical acute abdomen. Different kinds of pancreatitis may have different pathophysiological characteristics each other. The objective of this research was to investigate the early clinical features and complications of different types of acute pancreatitis. Methods. 787 AP patients admitted in the Huadu District People’s Hospital of Guangzhou during January 2009 and December 2019 were analyzed retrospectively. Among 787 AP patients, 520 (66.1%) were biliary AP (group I), 69 (8.7%) were alcoholic AP (group II), and 198 (25.2%) were hypertriglyceridemic AP (group III). According to the local and systemic complications and mortality in the early stage, we compared and analyzed the clinical characteristics and prognosis of different types of pancreatitis. Results. Mild acute pancreatitis accounted for the highest proportion (79.4%) in group I, while moderately severe acute pancreatitis in group II (36.2%) and severe acute pancreatitis in group III (62.6%). In terms of severity score of the pancreatitis, the average scores of BISAP, Ranson, APACHE-II, and MCTSI of the patients in group III were the highest ( p < 0.01 ). The incidence of acute peripancreatic fluid collection and infectious pancreatic necrosis was the highest in group III. The incidences of acute necrotic collection, pancreatic pseudocyst, and walled-off necrosis in group III were significantly higher than those in the other two groups ( p < 0.01 ). The incidences of systemic inflammatory response syndrome, sepsis, multiple organ failure, intra-abdominal hypertension, and mortality were highest in group III. Conclusions. There is an upward trend of the incidence rate of hypertriglyceridemic AP in recent years; it has been gradually developed into the second type of acute pancreatitis which is second only to the acute biliary pancreatitis. It is worthy to pay more and more attentions to it due to the feature of its younger onset, high incidence of complications, and high mortality.
- Published
- 2022
41. Uncovering the Effect and Mechanism of Panax Notoginseng Saponins on Metabolic Syndrome by Network Pharmacology Strategy
- Author
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Yisa Wang, Peng Ma, Zijing Wang, Mingxia Sun, Biyu Hou, Tianshu Xu, Wenlan Li, Xiuying Yang, Guanhua Du, Tengfei Ji, and Guifen Qiang
- Subjects
Blood Glucose ,Metabolic Syndrome ,Pharmacology ,Body Weight ,Insulins ,Panax notoginseng ,Water ,Network Pharmacology ,Saponins ,Lipids ,Mice, Inbred C57BL ,Mice ,Glucose ,Non-alcoholic Fatty Liver Disease ,Drug Discovery ,Animals ,Obesity ,RNA, Messenger ,Drugs, Chinese Herbal - Abstract
Metabolic syndrome (MetS) is a cluster of disease centered on obesity, which is the result of stagnation of liver qi according to traditional Chinese medicine. Panax notoginseng is a traditional Chinese herbal medicine, entering liver and stomach meridians and dissipating blood stasis, in which panax notoginseng saponins (PNS) are the main active components. However, its effects and mechanism on metabolic syndrome has not been revealed yet.To evaluate the anti-MetS effect of PNS, including body weight and adiposity, glucose metabolism and non-alcoholic fatty liver disease (NAFLD), as well as to explore the mechanism and signaling pathway of PNS on MetS effect.HPLC was utilized to affirm the percentages of saponins in PNS. In vivo, normal C57BL/6J mice and high-fat diet (HFD)-induced MetS mice were used to evaluate anti-MetS effect of PNS. Body weight, food and water intake were recorded. NMR imager was used for NMR imaging and lipid-water analysis. Blood glucose detection, glucose and insulin tolerance test were performed to evaluate glucose metabolism. Biochemical indexes analysis and histopathological staining were used to evaluate the effect on NAFLD. The expressions of mRNA and proteins related to thermogenesis in adipose tissue were determined using real-time PCR and Western blot. In silico, network pharmacology was utilized to predict potential mechanism. In vitro, matured 3T3-L1 adipocyte was used as subject to confirm the signaling pathway by Western blot.We determined the content of PNS component by HPLC. In vivo, PNS could improve metabolic syndrome with weight loss, reduction of adiposity, improvement of adipose distribution, correction of glucose metabolism disorder and attenuation of NAFLD. Mechanismly, PNS boosted energy exhaustion and dramatically enhanced thermogenesis in brown adipose tissue (BAT), induced white adipose tissue (WAT) browning. In silico, utilizing network pharmacology strategy, we identified 307 candidate targets which were enriched in MAPK signaling pathway specifically in liver tissue and adipocyte. In vitro validation confirmed ERK and p38MAPK mediated anti-MetS effects of PNS, not JNK signaling pathway.PNS exerted protective effect on metabolic syndrome through MAPK-mediated adipose thermogenic activation, which may serve as a prospective therapeutic drug for metabolic syndrome.
- Published
- 2022
42. Remote Nickel-Catalyzed Cross-Coupling Arylation via Proton-Coupled Electron Transfer-Enabled C–C Bond Cleavage
- Author
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Magnus Rueping, Long Huang, and Tengfei Ji
- Subjects
Chemistry ,chemistry.chemical_element ,General Chemistry ,Hydrogen atom ,010402 general chemistry ,Photochemistry ,01 natural sciences ,Biochemistry ,Chemical synthesis ,Catalysis ,0104 chemical sciences ,Coupling (electronics) ,Nickel ,Colloid and Surface Chemistry ,Direct coupling ,Proton-coupled electron transfer ,Bond cleavage - Abstract
Cross-coupling reactions for carbon-carbon and carbon-heteroatom bond formation are of great importance in modern chemical synthesis. In addition to classical cross-couplings involving preformed or preactivated coupling partners, more recently breakthroughs have been made in the selective, direct coupling of abundant aliphatic carbon-hydrogen bonds using hydrogen atom transfer reactions in which the bond-dissociation energy is the thermodynamic driving force. The more challenging carbon-carbon bond activation is still rather underdeveloped due to the bond inertness. Herein, we report a mild and general strategy for the activation of a diverse set of readily available cyclic alcohols for the remote and site-specific arylation of ketones via the combination of photoredox-mediated multisite concerted proton-electron transfer (MS-PCET) and nickel catalysis. The current cross-coupling proceeds with the generation of an alkoxy radical utilizing bond-dissociation free energy (BDFE) as the thermodynamic driving force. Subsequently, the resulting remote carbon-centered radicals formed by C-C cleavage merge with the nickel catalytic cycle to create the challenging C(sp
- Published
- 2020
43. SP/NK-1R Axis Promotes Perineural Invasion of Pancreatic Cancer and is Affected by lncRNA LOC389641
- Author
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Tengfei Ji, Keqiang Ma, Hongsheng Wu, and Tiansheng Cao
- Abstract
Background Pancreatic cancer is a deadly disease with low overall survival during the past 30 years. Perineural invasion (PNI) was considered to be the main reason for poor prognosis. In the present study, we analyzed the role of substance P (SP)/neurokinin-1 receptor (NK-1R) and long non-coding RNA (lncRNA) LOC389641 on pancreatic cancer PNI. Material and methods Pancreatic cancer cell lines of BxPC-3 and MIAPaCa-2 were co-cultured with SHSY5Y cells, and then stimulated with SP to simulate the in vivo influence of pancreatic cancer by ganglion. The co-culture cells were transfected with overexpressed neurokinin-1 receptor (NK-1R), silenced NK-1R, overexpressed LOC389641, and silenced LOC389641. Enzyme-linked immunosorbent assay (ELISA) assay was performed to examine the concentration of SP in cells. The cell proliferation ability was assessed by cell counting kit-8 and 5-ethynyl-2’-deoxyuridine (EdU) assays. The wound-healing and Transwell assays were carried out to determine the cell migration and invasion analyses. Quantitative real-time PCR (qRT-PCR), western blot, immunofluorescence (IF) analyses were performed to evaluate the expression levels. Results Addition of SP in co-culture system positively regulates cell proliferation, migration, and invasion of pancreatic cancer PNI. SP significantly stimulated NK-1R/Akt/NF-κB signaling pathway. The concentration of 100 nmol/L for 24h was chosen to be optimal for SP treatment.NK-1R positively regulated the proliferation, migration, and invasion of pancreatic cancer PNI. The expression of lncRNA LOC389641 and mRNA tumor necrosis factor receptor SF10A (TNFRSF10A) was not affected by SP. Overexpressed and silenced LOC389641 can correspondingly change the effect of SP stimulation on pancreatic cancer PNI. Conclusion We found that SP/NK-1R and LOC389641 promote the cell progression of pancreatic cancer PNI. Moreover, we assumed that the pancreatic cancer PNI promoted by SP/NK-1R axis may be blocked by the TNFRSF10A/NF-κB pathway mediated by LOC389641.
- Published
- 2021
44. Front Cover: Preparative isolation of antioxidative gallic acid derivatives from Saxifraga tangutica using a class separation method based on medium‐pressure liquid chromatography and reversed‐phase liquid chromatography
- Author
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Jun Dang, Qi Wang, Qilan Wang, Chen Yuan, Gang Li, and Tengfei Ji
- Subjects
Filtration and Separation ,Analytical Chemistry - Published
- 2021
45. Polycyclic polyprenylated acylphloroglucinols from Hypericum beanii and their hepatoprotective activity
- Author
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Yonghui Ma, Xinyue Suo, Xiaoxiu Li, Tingting Zhu, Jin Li, Tengfei Ji, and Bo Liu
- Subjects
Cyclopropanes ,Molecular Structure ,Plant Science ,General Medicine ,Phloroglucinol ,Horticulture ,Molecular Biology ,Biochemistry ,Hypericum ,Acetaminophen - Abstract
Twenty-seven polycyclic polyprenylated acylphloroglucinols (PPAPs) with diverse skeletons, including seven previously undescribed ones (hyperbeanins A-G), were isolated from the aerial parts of Hypericum beanii. Their structures were established by comprehensive analysis of NMR, HRESIMS, and experimental electronic circular dichroism (ECD) spectra. Hyperbeanin A was a monocyclic polyprenylated acylphloroglucinols (MPAPs) with an unusual spiro-fused cyclopropane ring. Four of the isolated compounds showed obvious hepatoprotective activity against paracetamol-induced HepG2 cell damage at 10 μM. The present results suggested that these compounds would be potential hepatoprotective agents. In addition, the plausible biogenetic pathways of hyperbeanins A-G were proposed, which gave an insight for future biomimetic synthesis of them.
- Published
- 2022
46. Tailoring coordination environments of single-atom electrocatalysts for hydrogen evolution by topological heteroatom transfer
- Author
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Sheng Qian, Feng Xu, Yu Fan, Ningyan Cheng, Huaiguo Xue, Ye Yuan, Romain Gautier, Tengfei Jiang, and Jingqi Tian
- Subjects
Science - Abstract
Abstract The rational design of carbon-supported transition-metal single-atom catalysts requires the precise arrangement of heteroatoms within the single-atom catalysts. However, achieving this design is challenging due to the collapse of the structure during the pyrolysis. Here, we introduce a topological heteroatom-transfer strategy to prevent the collapse and accurately control the P coordination in carbon-supported single-atom catalysts. As an illustration, we have prepared self-assembled helical fibers with encapsulated cavities. Within these cavities, adjustable functional groups can chelate metal ions (Nx···Mn+···Oy), facilitating the preservation of the structure during the pyrolysis based phosphidation. This process allows for the transfer of heteroatoms from the assembly into single-atom catalysts, resulting in the precise coordination tailoring. Notably, the Co–P2N2–C catalyst exhibits electrocatalytic performance as a non-noble metal single-atom catalyst for alkaline hydrogen evolution, attaining a current density of 100 mA cm−2 with an overpotential of only 131 mV.
- Published
- 2024
- Full Text
- View/download PDF
47. Hyperacmosins K-M, three new polycyclic polyprenylated acylphloroglucinols from
- Author
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Mingxia, Sun, Xue, Wang, Tingting, Zhu, Xinyue, Suo, Jiajia, Wang, Tengfei, Ji, and Bo, Liu
- Abstract
Hyperacmosins K-M (1-3), three new polycyclic polyprenylated acylphloroglucinol (PPAPs) derivatives, were isolated from the air-dried aerial parts of
- Published
- 2021
48. PADI1 contributes to epithelial-mesenchymal transition in pancreatic ductal adenocarcinoma through activating ERK1/2-p38 signaling pathway
- Author
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Liang Chen, Tengfei Ji, Tiansheng Cao, and Keqiang Ma
- Subjects
Pancreatic ductal adenocarcinoma ,Chemistry ,PADI1 ,p38 mitogen-activated protein kinases ,Cancer research ,Epithelial–mesenchymal transition ,Signal transduction - Abstract
Background Peptidylarginine deiminase 1 (PADI1) may be relative with the progression of epithelial-mesenchymal transition (EMT) in pancreatic ductal adenocarcinoma (PAAD). We aim to explore the role of PADI1 in PAAD. Methods The expression pattern of PADI1 in PAAD tissues and normal tissues was analyzed using The Cancer Genome Atlas (TCGA) dataset. PADI1 was knocked down in CFPAN-1 and HPAC cells, while overexpressed in PANC-1 and Bxpc-3 cells by RNA interference. Wound healing assay was performed to analyze relative cell migration distance. Cell migration and invasion were assessed by Transwell assay. Related protein expression levels were measured by western blot and immunofluorescence. Results Bioinformatics analysis showed that PADI1 was overexpressed in PAAD tissues and associated with worse survival prognosis. Knockdown of PADI1 suppressed the cell migration, invasion and activated ERK1/2-p38 signaling pathway in CFPAN-1 and HPAC cells. Overexpression of PADI1 obtained the opposite results in PANC-1 and Bxpc-3 cells. Moreover, treatment with MEK1/2 inhibitor significantly recovered the effects of PADI1 knockdown on cell migration, invasion, EMT process and p-ERK1/2 and p38 expression in CFPAN-1 and HPAC cells. Conclusions Our data suggested that PADI1 may function as an oncogene in regulating metastasis in vitro in PAAD.
- Published
- 2021
49. The performance tests of three-dimensional printing titanium alloy craniomaxillofacial bone plate: A preliminary preclinical study
- Author
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Hongpu Wei, Xiaochen Xue, Jingshuang Yu, Tengfei Jiang, Xiang Li, Kaili Lin, Lei Zhang, and Xudong Wang
- Subjects
Biomechanical properties ,Craniomaxillofacial bone plate ,Fatigue properties ,Morphological properties ,Three-dimensional printing ,Dentistry ,RK1-715 - Abstract
Background/purpose: Because of the complex anatomical structure of the maxillofacial skeleton, bending plates is necessary during surgery. The fast developing three-dimensional printing (3DP) technology has provided a new method for making personalized craniomaxillofacial bone plates. However, the properties of these bone plates remain unknown. This study evaluates the mechanical, fatigue, and morphological properties of these bone plates, which may provide data supporting future clinical applications. Materials and methods: The 3DP bone plate was fabricated by selective laser melting (SLM) and electron beam melting (EBM) technologies. Mechanical, surface, and defect analyses were performed to compare their properties with a standard machined sample. One-way analysis of variance was applied, with p
- Published
- 2023
- Full Text
- View/download PDF
50. Reliability Risk Mitigation in Advanced Packages by Aging-Induced Precipitation of Bi in Water-Quenched Sn–Ag–Cu–Bi Solder
- Author
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Vishnu Shukla, Omar Ahmed, Peng Su, and Tengfei Jiang
- Subjects
lead-free solder ,SAC+X ,solder interconnects ,quasistatic nanoindentation ,continuous stiffness measurement ,intermetallic compound ,Technology ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Microscopy ,QH201-278.5 ,Descriptive and experimental mechanics ,QC120-168.85 - Abstract
Bi-doped Sn–Ag–Cu (SAC) microelectronic solder is gaining attention for its utility as a material for solder joints that connect substrates to printed circuit boards (PCB) in future advanced packages, as Bi-doped SAC is reported to have a lower melting temperature, higher strength, higher wettability on conducting pads, and lower intermetallic compound (IMC) formation at the solder-pad interface. As solder joints are subjected to aging during their service life, an investigation of aging-induced changes in the microstructure and mechanical properties of the solder alloy is needed before its wider acceptance in advanced packages. This study focuses on the effects of 1 to 3 wt.% Bi doping in an Sn–3.0Ag–0.5Cu (SAC305) solder alloy on aging-induced changes in hardness and creep resistance for samples prepared by high cooling rates (>5 °C/s). The specimens were aged at ambient and elevated temperatures for up to 90 days and subjected to quasistatic nanoindentation to determine hardness and nanoscale dynamic nanoindentation to determine creep behavior. The microstructural evolution was investigated with a scanning electron microscope in tandem with energy-dispersive spectroscopy to correlate with aging-induced property changes. The hardness and creep strength of the samples were found to increase as the Bi content increased. Moreover, the hardness and creep strength of the 0–1 wt.% Bi-doped SAC305 was significantly reduced with aging, while that of the 2–3 wt.% Bi-doped SAC305 increased with aging. The changes in these properties with aging were correlated to the interplay of multiple hardening and softening mechanisms. In particular, for 2–3 wt.% Bi, the enhanced performance was attributed to the potential formation of additional Ag3Sn IMCs with aging due to non-equilibrium solidification and the more uniform distribution of Bi precipitates. The observations that 2–3 wt.% Bi enhances the hardness and creep strength of the SAC305 alloy with isothermal aging to mitigate reliability risks is relevant for solder samples prepared using high cooling rates.
- Published
- 2024
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