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3 results on '"Tereza Batkova"'

1. Dynamic release of neuronal extracellular vesicles containing miR‐21a‐5p is induced by hypoxia

Author

Nea Korvenlaita, Mireia Gómez‐Budia, Flavia Scoyni, Cristiana Pistono, Luca Giudice, Shaila Eamen, Sanna Loppi, Ana Hernández deSande, Benjamin Huremagic, Maria Bouvy‐Liivrand, Merja Heinäniemi, Minna U. Kaikkonen, Lesley Cheng, Andrew F. Hill, Katja M. Kanninen, Guido W. Jenster, Martin E. vanRoyen, Laura Ramiro, Joan Montaner, Tereza Batkova, Robert Mikulik, Rosalba Giugno, Jukka Jolkkonen, Paula Korhonen, and Tarja Malm

Subjects
biomarkers, extracellular vesicle, hypoxia, ischemic stroke, miR‐21a‐5p, neuron, Cytology, QH573-671
Abstract

Abstract Hypoxia induces changes in the secretion of extracellular vesicles (EVs) in several non‐neuronal cells and pathological conditions. EVs are packed with biomolecules, such as microRNA(miR)‐21‐5p, which respond to hypoxia. However, the true EV association of miR‐21‐5p, and its functional or biomarker relevance, are inadequately characterised. Neurons are extremely sensitive cells, and it is not known whether the secretion of neuronal EVs and miR‐21‐5p are altered upon hypoxia. Here, we characterised the temporal EV secretion profile and cell viability of neurons under hypoxia. Hypoxia induced a rapid increase of miR‐21a‐5p secretion in the EVs, which preceded the elevation of hypoxia‐induced tissue or cellular miR‐21a‐5p. Prolonged hypoxia induced cell death and the release of morphologically distinct EVs. The EVs protected miR‐21a‐5p from enzymatic degradation but a remarkable fraction of miR‐21a‐5p remained fragile and non‐EV associated. The increase in miR‐21a‐5p secretion may have biomarker potential, as high blood levels of miR‐21‐5p in stroke patients were associated with significant disability at hospital discharge. Our data provides an understanding of the dynamic regulation of EV secretion from neurons under hypoxia and provides a candidate for the prediction of recovery from ischemic stroke.

Published
2023
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2. Increased Occurrence of Treponema spp. and Double-species Infections in Patients with Alzheimer’s Disease

Author

Jiri Damborsky, Andrea Schenkmayerova, Martin Marek, Tereza Batkova, Martina Hlozankova, Barbora Liskova, Katerina Sheardova, Radoslav Matej, Michal Nemergut, Dana Vigasova, M. Bartos, Jan Laczó, Michal Sitina, Jakub Hort, Martin Vyhnalek, Radim Jancalek, and Ingrid Kovacova

Subjects
Human cytomegalovirus, 0303 health sciences, Treponema, biology, business.industry, Disease, medicine.disease, biology.organism_classification, 3. Good health, 03 medical and health sciences, Exact test, 0302 clinical medicine, Cerebrospinal fluid, Immunology, Multiplex polymerase chain reaction, Medicine, In patient, Borrelia burgdorferi, business, 030217 neurology & neurosurgery, 030304 developmental biology
Abstract

ObjectiveAlthough the link between microbial infections and Alzheimer’s disease (AD) has been demonstrated in multiple studies, the involvement of pathogens in the development of AD remains unclear. Therefore, this theory beckons further systematic investigation. In this study, we have examined the association between the 10 most widely discussed viral and bacterial pathogens found in serum and cerebrospinal fluid (CSF) from patients with AD.MethodsWe have used an in-house developed multiplex PCR kit for simultaneous detection of five bacterial and five viral pathogens in serum and CSF from 50 AD patients and 53 healthy controls. Data analysis was performed with multiple statistical methods: Fisher’s exact test, chisquare goodness of fit test, and one-sample proportion test.ResultsWe observed an increased frequency of AD patients tested positive for Treponema spp. (AD: 62.2%; CTRL: 30.3%; p-value = 0.007). Furthermore, we confirmed a significantly higher prevalence of cases with two and more simultaneous infections in AD patients compared to controls (AD: 24%; CTRL 7.5%; p-value = 0.029). The studied pathogens were widespread equally in serum and CSF. Borrelia burgdorferi, human herpesvirus 7, and human cytomegalovirus were not detected in any of the studied samples.DiscussionAn increased prevalence of Treponema spp. and double-species infections in AD patients compared to the healthy controls provides further evidence of the association between microbial infections and AD. Paralleled analysis of multiple sample specimens provides complementary information and is advisable for future studies.

Published
2021
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3. Increased occurrence of Treponema spp. and double-species infections in patients with Alzheimer's disease

Author

Michal Nemergut, Tereza Batkova, Dana Vigasova, Milan Bartos, Martina Hlozankova, Andrea Schenkmayerova, Barbora Liskova, Katerina Sheardova, Martin Vyhnalek, Jakub Hort, Jan Laczó, Ingrid Kovacova, Michal Sitina, Radoslav Matej, Radim Jancalek, Martin Marek, and Jiri Damborsky

Subjects
Environmental Engineering, Treponemal Infections, Alzheimer Disease, Case-Control Studies, Herpesvirus 6, Human, Humans, Environmental Chemistry, Treponema, Pollution, Waste Management and Disposal
Abstract

Although the link between microbial infections and Alzheimer's disease (AD) has been demonstrated in multiple studies, the involvement of pathogens in the development of AD remains unclear. Here, we investigated the frequency of the 10 most commonly cited viral (HSV-1, EBV, HHV-6, HHV-7, and CMV) and bacterial (Chlamydia pneumoniae, Helicobacter pylori, Borrelia burgdorferi, Porphyromonas gingivalis, and Treponema spp.) pathogens in serum, cerebrospinal fluid (CSF) and brain tissues of AD patients. We have used an in-house multiplex PCR kit for simultaneous detection of five bacterial and five viral pathogens in serum and CSF samples from 50 AD patients and 53 healthy controls (CTRL). We observed a significantly higher frequency rate of AD patients who tested positive for Treponema spp. compared to controls (AD: 62.2 %; CTRL: 30.3 %; p-value = 0.007). Furthermore, we confirmed a significantly higher occurrence of cases with two or more simultaneous infections in AD patients compared to controls (AD: 24 %; CTRL 7.5 %; p-value = 0.029). The studied pathogens were detected with comparable frequency in serum and CSF. In contrast, Borrelia burgdorferi, human herpesvirus 7, and human cytomegalovirus were not detected in any of the studied samples. This study provides further evidence of the association between microbial infections and AD and shows that paralleled analysis of multiple sample specimens provides complementary information and is advisable for future studies.

Published
2022
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