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Your search keyword '"Terkawi, M. A."' showing total 24 results
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24 results on '"Terkawi, M. A."'

9. Expression of Babesia gibsoni thrombospondin-related adhesive protein in Toxoplasma gondii and evaluation of its antigenicity and immunogenicity

Author

Kumagai, A., Zhang, G., Jia, H., Nakamura, C., Zhang, H., Goo, Y-K., Aboge, G. A., Terkawi, M. A., Zhou, J., Nishikawa, Yoshifumi, and Xuan, Xuenan

Subjects
BgTRAP, Babesia gibsoni, vaccine, parasitic diseases, expression, Toxoplasma gondii
Abstract

application/pdf, A gene encoding Babesia gibsoni thrombospondin-related adhesive protein (BgTRAP), known as a vaccine candidate, was stably expressed in Toxoplasma gondii (Tg/BgTRAP). The molecular weight and the antigenic reaction of recombinant BgTRAP expressed by the Tg/BgTRAP were similar to the original ones expressed by B. gibsoni. To evaluate the antigenicity of the recombinant BgTRAP expressed by T. gondii, the lysates of the recombinant parasite tachyzoites were intraperitoneally injected into mice. The serum collected from Tg/BgTRAP-immunized mouse could react to B. gibsoni parasites, while the serum collected from wild-type T. gondii tachyzoites (Tg/wt)-immunized mice did not. These results indicate that T. gondii could provide a new tool to produce foreign antigens from other protozoan parasites and the recombinant BgTRAP expressed by T. gondii might be a useful antigen for developing a diagnostic reagent and vaccine to control canine babesiosis.

Published
2016

11. Artesunate, a potential drug for treatment of Babesia infection

Author

Goo, Youn-Kyoung, Terkawi, M. Alaa, Jia, Honglin, Aboge, G. Oluga, Ooka, Hideo, Kim, Suk, Igarashi, Ikuo, Nishikawa, Yoshifumi, and Xuan, Xuenan

Subjects
animal diseases, Babesia gibsoni, parasitic diseases, Babesia bovis, Artesunate, Babesia microti
Abstract

application/pdf, The effect of artesunate, a water-soluble artemisinin derivative, against Babesia species, such as Babesia bovis, Babesia gibsoni, and Babesia microti was studied. Cultures of B. bovis and B. gibsoni were treated with 0.26 μM, 2.6 μM, 26 μM, and 260 μM artesunate, and the growth-inhibitory effect was shown in over 2.6 μM artesunate in day 4 and day 3 post-subculture for B. bovis and B. gibsoni, respectively, in dose-dependent manner. In vivo experiment for B. microti, strong inhibition effects were observed in mouse groups treated with over 1.0 mg/kg body weight of artesunate on day 9 and 10 post-infection. These results suggest that artesunate could be a potential drug for Babesia infection.

Published
2010

13. Identification of a novel B. gibsoni 27-kDa protein as a serodiagnostic antigen

Author

Terkawi, M. A., Aboge, G., Jia, H., Goo, Y-K., Ooka, H., Yamagishi, Junya, Nishikawa, Yoshifumi, Kawazu, Shin-ichiro, Fujisaki, K., and Xuan, Xuenan

Subjects
Babesia gibsoni, deiagnostic performance, enzyme-linked immunosorbent assay
Abstract

application/pdf, A novel gene encoding 27-kDa protein was identified by the screening of Babesia gibsoni cDNA library with acutely infected dog serum. The BgP27 is a single copy gene with a predicted open reading frame of 762 bp and 254 amino acids. The phylogenic analysis of the deduced amino acid of BgP27 demonstrated considerable identities with members of Plasmodium berghei circumsporozoite protein family that ranged between 18.4% and 22.8%. The BgP27 was expressed as a glutathione S-transferase fusion protein in Escherichia coli. The serum raised in mice against the recombinant protein specifically reacted with a 27-kDa protein in the extracts of B. gibsoni parasites. Confocal laser scanning microscopic observation showed high fluorescent reactivity with both extracellular and intracellular merozoite. Furthermore, recombinant BgP27 was used as an antigen in an enzyme-linked immunosorbent assay (ELISA). Thus, the kinetics of the anti-BgP27 antibody was detected in serial serum samples collected from a B. gibsoni-infected dog. IgG levels were high throughout the course of infection. In addition, the ELISA was able to differentiate between B. gibsoni-infected dog serum and B. canis subspecies-infected dog serum or normal dog serum. The diagnostic performance of BgP27-ELISA revealed the potential use of the antigen for detection of infection in dogs.

Published
2008

14. Four promising antigens, BgP32, BgP45, BgP47, and BgP50, for serodiagnosis of Babesia gibsoni infection were classified as B. gibsoni merozoite surface protein family

Author

Goo, Youn-Kyoung, primary, Aboge, Gabriel Oluga, additional, Terkawi, M. Alaa, additional, Jia, Honglin, additional, Yamagishi, Junya, additional, Sunaga, Fujiko, additional, Namikawa, Kazuhiko, additional, Cha, Se-Yeoun, additional, Jang, Hyung-Kwan, additional, Kim, Suk, additional, Nishikawa, Yoshifumi, additional, and Xuan, Xuenan, additional

Published
2012
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15. Identification and characterization of a novel secreted antigen 1 of Babesia microti and evaluation of its potential use in enzyme-linked immunosorbent assay and immunochromatographic test

Author

Luo, Yuzi, primary, Jia, Honglin, additional, Terkawi, M. Alaa, additional, Goo, Youn-Kyoung, additional, Kawano, Suguru, additional, Ooka, Hideo, additional, Li, Yan, additional, Yu, Longzheng, additional, Cao, Shinuo, additional, Yamagishi, Junya, additional, Fujisaki, Kozo, additional, Nishikawa, Yoshifumi, additional, Saito-Ito, Atsuko, additional, Igarashi, Ikuo, additional, and Xuan, Xuenan, additional

Published
2011
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18. Identification of Secreted Antigen 3 from Babesia gibsoni

Author

Jia, Honglin, primary, Terkawi, M. Alaa, additional, Aboge, Gabriel Oluga, additional, Goo, Youn-Kyoung, additional, Ma, Liqing, additional, Zhou, Jinlin, additional, Nishikawa, Yoshifumi, additional, Igarashi, Ikuo, additional, Fujisaki, Kozo, additional, and Xuan, Xuenan, additional

Published
2009
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19. Genetic diversity of two selected antigen loci in Babesia gibsoni Asian genotype obtained from Japan and Jeju island of South Korea

Author

Jia, Honglin, primary, Aboge, Gabriel Oluga, additional, Terkawi, M. Alaa, additional, Goo, Youn-Kyoung, additional, Nishikawa, Yoshifumi, additional, Kuriki, Ken, additional, Lee, Kyoung-Kap, additional, Jang, Hyung-Kwan, additional, Kim, Suk, additional, Fujisaki, Kozo, additional, and Xuan, Xuenan, additional

Published
2009
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20. Characterization of the Babesia gibsoni 12-kDa protein as a potential antigen for the serodiagnosis

Author

Goo, Youn-Kyoung, primary, Jia, Honglin, additional, Aboge, G. Oluga, additional, Terkawi, M. Alaa, additional, Lee, Eung-goo, additional, Yamagishi, Junya, additional, Nishikawa, Yoshifumi, additional, Jang, Hyung-Kwan, additional, Sunaga, Fujiko, additional, Namikawa, Kazuhiko, additional, Fujisaki, Kozo, additional, and Xuan, Xuenan, additional

Published
2009
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21. Babesia gibsoni: Serodiagnosis of infection in dogs by an enzyme-linked immunosorbent assay with recombinant BgTRAP

Author

Goo, Youn-Kyoung, primary, Jia, Honglin, additional, Aboge, G. Oluga, additional, Terkawi, M. Alaa, additional, Kuriki, Ken, additional, Nakamura, Chinatsu, additional, Kumagai, Akiko, additional, Zhou, Jinlin, additional, Lee, Eung-goo, additional, Nishikawa, Yoshifumi, additional, Igarashi, Ikuo, additional, Fujisaki, Kozo, additional, and Xuan, Xuenan, additional

Published
2008
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23. Molecular and immunological characterization of Babesia gibsoni and Babesia microti heat shock protein-70.

Author

TERKAWI, M. ALAA, ABOGE, G., JIA, H., GOO, Y.-K., OOKA, H., YAMAGISHI, J., NISHIKAWA, Y., YOKOYAMA, N., IGARASHI, I., KAWAZU, S.-I., FUJISAKI, K., and XUAN, X.

Subjects
*HEAT shock proteins, *PARASITES, *VACCINATION, *PREVENTIVE medicine, *IMMUNOLOGICAL adjuvants
Abstract

Serological immunoscreening was used to identify a gene encoding heat shock protein-70 from Babesia gibsoni (BgHSP-70) that showed high homology with HSP-70s from other apicomplexan parasites. This gene corresponded to a full-length cDNA containing an open reading frame of 1968 bp predicted to result in a 70-kDa mature protein consisting of 656 amino acids. Analysis of the expression levels of BgHSP-70 indicated elevated transcription from cultured parasites incubated at 40°C for 1 h, but not at 30°C. Interestingly, antiserum raised against recombinant BgHSP-70 protein reacted specifically not only with a 70-kDa protein of B. gibsoni but also with a corresponding native protein of B. microti (BmHSP-70), indicating the high degree of conservation of this protein. The BmHSP-70 gene was then isolated and characterized and the immunoprotective properties of recombinant BgHSP-70 (rBgHSP-70) and rBmHSP-70 were compared in vitro and in vivo. Both proteins had potent mitogenic effects on murine and canine mononuclear cells as evidenced by high proliferative responses and IFN-γ production after stimulation. Immunization regimes in BALB/c and C57BL/6 mice using rBgHSP-70 and rBmHSP-70 elicited high antibody levels, with concurrent significant reductions in peripheral parasitaemias. Taken together, these results emphasize the potential of HSP-70s as a molecular adjuvant vaccine. [ABSTRACT FROM AUTHOR]

Published
2009
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