1. Bioinformatics investigation of the prognostic value and mechanistic role of CD9 in glioma.
- Author
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Jiang J, Jiang B, and Li WB
- Subjects
- Humans, Prognosis, Biomarkers, Tumor metabolism, Biomarkers, Tumor genetics, Cell Line, Tumor, Mutation, Glioma genetics, Glioma metabolism, Glioma pathology, Tetraspanin 29 metabolism, Tetraspanin 29 genetics, Computational Biology methods, Gene Expression Regulation, Neoplastic, Brain Neoplasms genetics, Brain Neoplasms metabolism, Brain Neoplasms pathology
- Abstract
In recent years, CD9 has been extensively studied as a potential biomarker for cancer. However, the biological role of CD9 in gliomas remains unclear. This study investigates the function of CD9 in gliomas and its molecular mechanisms. Utilizing pan-cancer analysis with TCGA, CGGA, and GEO databases, differential expression of CD9 was observed in 11 tumor types within the TCGA cohort, and it was associated with patient survival rates. Analysis of the CGGA glioma database revealed that patients with high CD9 expression had lower survival rates. The area under the ROC curve (AUC) for GSE16011 was greater than 0.7, indicating a high discriminative ability. Through gene set enrichment analysis (GSEA), immune-related analysis, and CD9 mutation detection, CD9 was found to have the strongest correlation with neutrophil involvement (cor = 0.30, P < 0.05), and the high CD9 expression group exhibited higher rejection responses and TIDE scores, suggesting a lower likelihood of successful immunotherapy. The high CD9 expression group was more sensitive to 81 drugs, indicating potential therapeutic effects for gliomas. Furthermore, overexpression of CD9 in gliomas may be associated with gene mutations. Down-regulation or up-regulation of CD9 expression in the glioblastoma cell line LN229 showed that CD9 could positively regulate the migratory ability of LN229 cells. Further, several marker genes, such as VEGFR-2, TGF-β1, CASP1 and PI3K, were down regulated in CD9 knockdown cell lines and up regulated in CD9 overexpression cell lines, compared with control cell line. This study preliminarily explores the role of CD9 in gliomas and its prognostic value, providing new insights for personalized treatment strategies in glioma therapy., (© 2024. The Author(s).)
- Published
- 2024
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