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1. De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals

Author

Tetreau, Guillaume, Sawaya, Michael R, De Zitter, Elke, Andreeva, Elena A, Banneville, Anne-Sophie, Schibrowsky, Natalie A, Coquelle, Nicolas, Brewster, Aaron S, Grünbein, Marie Luise, Kovacs, Gabriela Nass, Hunter, Mark S, Kloos, Marco, Sierra, Raymond G, Schiro, Giorgio, Qiao, Pei, Stricker, Myriam, Bideshi, Dennis, Young, Iris D, Zala, Ninon, Engilberge, Sylvain, Gorel, Alexander, Signor, Luca, Teulon, Jean-Marie, Hilpert, Mario, Foucar, Lutz, Bielecki, Johan, Bean, Richard, de Wijn, Raphael, Sato, Tokushi, Kirkwood, Henry, Letrun, Romain, Batyuk, Alexander, Snigireva, Irina, Fenel, Daphna, Schubert, Robin, Canfield, Ethan J, Alba, Mario M, Laporte, Frédéric, Després, Laurence, Bacia, Maria, Roux, Amandine, Chapelle, Christian, Riobé, François, Maury, Olivier, Ling, Wai Li, Boutet, Sébastien, Mancuso, Adrian, Gutsche, Irina, Girard, Eric, Barends, Thomas RM, Pellequer, Jean-Luc, Park, Hyun-Woo, Laganowsky, Arthur D, Rodriguez, Jose, Burghammer, Manfred, Shoeman, Robert L, Doak, R Bruce, Weik, Martin, Sauter, Nicholas K, Federici, Brian, Cascio, Duilio, Schlichting, Ilme, and Colletier, Jacques-Philippe

Subjects
Inorganic Chemistry, Biochemistry and Cell Biology, Chemical Sciences, Biological Sciences, Animals, Bacillus thuringiensis, Bacterial Proteins, Endotoxins, Hemolysin Proteins, Larva, Mosquito Control, Nanoparticles
Abstract

Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis and jegathesan, respectively. The toxins naturally crystallize within the host; however, the crystals are too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography at X-ray free electron lasers to in vivo-grown nanocrystals of these toxins. The structure of Cry11Aa was determined de novo using the single-wavelength anomalous dispersion method, which in turn enabled the determination of the Cry11Ba structure by molecular replacement. The two structures reveal a new pattern for in vivo crystallization of Cry toxins, whereby each of their three domains packs with a symmetrically identical domain, and a cleavable crystal packing motif is located within the protoxin rather than at the termini. The diversity of in vivo crystallization patterns suggests explanations for their varied levels of toxicity and rational approaches to improve these toxins for mosquito control.

Published
2022

2. De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals

Author

Tetreau, Guillaume, Sawaya, Michael R, De Zitter, Elke, Andreeva, Elena A, Banneville, Anne-Sophie, Schibrowsky, Natalie, Coquelle, Nicolas, Brewster, Aaron S, Grünbein, Marie Luise, Kovacs, Gabriela Nass, Hunter, Mark S, Kloos, Marco, Sierra, Raymond G, Schiro, Giorgio, Qiao, Pei, Stricker, Myriam, Bideshi, Dennis, Young, Iris D, Zala, Ninon, Engilberge, Sylvain, Gorel, Alexander, Signor, Luca, Teulon, Jean-Marie, Hilpert, Mario, Foucar, Lutz, Bielecki, Johan, Bean, Richard, de Wijn, Raphael, Sato, Tokushi, Kirkwood, Henry, Letrun, Romain, Batyuk, Alexander, Snigireva, Irina, Fenel, Daphna, Schubert, Robin, Canfield, Ethan J, Alba, Mario M, Laporte, Frédéric, Després, Laurence, Bacia, Maria, Roux, Amandine, Chapelle, Christian, Riobé, François, Maury, Olivier, Ling, Wai Li, Boutet, Sébastien, Mancuso, Adrian, Gutsche, Irina, Girard, Eric, Barends, Thomas RM, Pellequer, Jean-Luc, Park, Hyun-Woo, Laganowsky, Arthur D, Rodriguez, Jose, Burghammer, Manfred, Shoeman, Robert L, Doak, R Bruce, Weik, Martin, Sauter, Nicholas K, Federici, Brian, Cascio, Duilio, Schlichting, Ilme, and Colletier, Jacques-Philippe

Subjects
Inorganic Chemistry, Biochemistry and Cell Biology, Chemical Sciences, Biological Sciences
Abstract

Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis and jegathesan , respectively. The toxins naturally crystallize within the host; however, the crystals are too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography at X-ray free electron lasers to in vivo -grown nanocrystals of these toxins. The structure of Cry11Aa was determined de novo using the single-wavelength anomalous dispersion method, which in turn enabled the determination of the Cry11Ba structure by molecular replacement. The two structures reveal a new pattern for in vivo crystallization of Cry toxins, whereby each of their three domains packs with a symmetrically identical domain, and a cleavable crystal packing motif is located within the protoxin rather than at the termini. The diversity of in vivo crystallization patterns suggests explanations for their varied levels of toxicity and rational approaches to improve these toxins for mosquito control.

Published
2021

3. Serial femtosecond crystallography on in vivo-grown crystals drives elucidation of mosquitocidal Cyt1Aa bioactivation cascade.

Author

Tetreau, Guillaume, Banneville, Anne-Sophie, Andreeva, Elena A, Brewster, Aaron S, Hunter, Mark S, Sierra, Raymond G, Teulon, Jean-Marie, Young, Iris D, Burke, Niamh, Grünewald, Tilman A, Beaudouin, Joël, Snigireva, Irina, Fernandez-Luna, Maria Teresa, Burt, Alister, Park, Hyun-Woo, Signor, Luca, Bafna, Jayesh A, Sadir, Rabia, Fenel, Daphna, Boeri-Erba, Elisabetta, Bacia, Maria, Zala, Ninon, Laporte, Frédéric, Després, Laurence, Weik, Martin, Boutet, Sébastien, Rosenthal, Martin, Coquelle, Nicolas, Burghammer, Manfred, Cascio, Duilio, Sawaya, Michael R, Winterhalter, Mathias, Gratton, Enrico, Gutsche, Irina, Federici, Brian, Pellequer, Jean-Luc, Sauter, Nicholas K, and Colletier, Jacques-Philippe

Subjects
NIH 3T3 Cells, Cell Membrane, Animals, Humans, Mice, Disulfides, Bacterial Proteins, Endotoxins, Insecticides, Microscopy, Atomic Force, Crystallography, X-Ray, Protein Conformation, Hydrogen-Ion Concentration, Hemolysin Proteins, HEK293 Cells, Sf9 Cells, Microscopy, Atomic Force, Crystallography, X-Ray
Abstract

Cyt1Aa is the one of four crystalline protoxins produced by mosquitocidal bacterium Bacillus thuringiensis israelensis (Bti) that has been shown to delay the evolution of insect resistance in the field. Limiting our understanding of Bti efficacy and the path to improved toxicity and spectrum has been ignorance of how Cyt1Aa crystallizes in vivo and of its mechanism of toxicity. Here, we use serial femtosecond crystallography to determine the Cyt1Aa protoxin structure from sub-micron-sized crystals produced in Bti. Structures determined under various pH/redox conditions illuminate the role played by previously uncharacterized disulfide-bridge and domain-swapped interfaces from crystal formation in Bti to dissolution in the larval mosquito midgut. Biochemical, toxicological and biophysical methods enable the deconvolution of key steps in the Cyt1Aa bioactivation cascade. We additionally show that the size, shape, production yield, pH sensitivity and toxicity of Cyt1Aa crystals grown in Bti can be controlled by single atom substitution.

Published
2020

6. Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta

Author

Kanost, Michael R, Arrese, Estela L, Cao, Xiaolong, Chen, Yun-Ru, Chellapilla, Sanjay, Goldsmith, Marian R, Grosse-Wilde, Ewald, Heckel, David G, Herndon, Nicolae, Jiang, Haobo, Papanicolaou, Alexie, Qu, Jiaxin, Soulages, Jose L, Vogel, Heiko, Walters, James, Waterhouse, Robert M, Ahn, Seung-Joon, Almeida, Francisca C, An, Chunju, Aqrawi, Peshtewani, Bretschneider, Anne, Bryant, William B, Bucks, Sascha, Chao, Hsu, Chevignon, Germain, Christen, Jayne M, Clarke, David F, Dittmer, Neal T, Ferguson, Laura CF, Garavelou, Spyridoula, Gordon, Karl HJ, Gunaratna, Ramesh T, Han, Yi, Hauser, Frank, He, Yan, Heidel-Fischer, Hanna, Hirsh, Ariana, Hu, Yingxia, Jiang, Hongbo, Kalra, Divya, Klinner, Christian, König, Christopher, Kovar, Christie, Kroll, Ashley R, Kuwar, Suyog S, Lee, Sandy L, Lehman, Rüdiger, Li, Kai, Li, Zhaofei, Liang, Hanquan, Lovelace, Shanna, Lu, Zhiqiang, Mansfield, Jennifer H, McCulloch, Kyle J, Mathew, Tittu, Morton, Brian, Muzny, Donna M, Neunemann, David, Ongeri, Fiona, Pauchet, Yannick, Pu, Ling-Ling, Pyrousis, Ioannis, Rao, Xiang-Jun, Redding, Amanda, Roesel, Charles, Sanchez-Gracia, Alejandro, Schaack, Sarah, Shukla, Aditi, Tetreau, Guillaume, Wang, Yang, Xiong, Guang-Hua, Traut, Walther, Walsh, Tom K, Worley, Kim C, Wu, Di, Wu, Wenbi, Wu, Yuan-Qing, Zhang, Xiufeng, Zou, Zhen, Zucker, Hannah, Briscoe, Adriana D, Burmester, Thorsten, Clem, Rollie J, Feyereisen, René, Grimmelikhuijzen, Cornelis JP, Hamodrakas, Stavros J, Hansson, Bill S, Huguet, Elisabeth, Jermiin, Lars S, Lan, Que, Lehman, Herman K, Lorenzen, Marce, Merzendorfer, Hans, Michalopoulos, Ioannis, Morton, David B, Muthukrishnan, Subbaratnam, Oakeshott, John G, Palmer, Will, Park, Yoonseong, and Passarelli, A Lorena

Subjects
Biological Sciences, Bioinformatics and Computational Biology, Genetics, Tobacco, 1.1 Normal biological development and functioning, Underpinning research, Generic health relevance, Animals, Gene Expression, Gene Expression Profiling, Genome, Insect, Larva, Manduca, Pupa, Sequence Analysis, DNA, Synteny, Lepidoptera, Insect, Tobacco hornworm, Moth, Insect biochemistry, Innate immunity, Medicinal and Biomolecular Chemistry, Biochemistry and Cell Biology, Zoology, Entomology, Biochemistry and cell biology
Abstract

Manduca sexta, known as the tobacco hornworm or Carolina sphinx moth, is a lepidopteran insect that is used extensively as a model system for research in insect biochemistry, physiology, neurobiology, development, and immunity. One important benefit of this species as an experimental model is its extremely large size, reaching more than 10 g in the larval stage. M. sexta larvae feed on solanaceous plants and thus must tolerate a substantial challenge from plant allelochemicals, including nicotine. We report the sequence and annotation of the M. sexta genome, and a survey of gene expression in various tissues and developmental stages. The Msex_1.0 genome assembly resulted in a total genome size of 419.4 Mbp. Repetitive sequences accounted for 25.8% of the assembled genome. The official gene set is comprised of 15,451 protein-coding genes, of which 2498 were manually curated. Extensive RNA-seq data from many tissues and developmental stages were used to improve gene models and for insights into gene expression patterns. Genome wide synteny analysis indicated a high level of macrosynteny in the Lepidoptera. Annotation and analyses were carried out for gene families involved in a wide spectrum of biological processes, including apoptosis, vacuole sorting, growth and development, structures of exoskeleton, egg shells, and muscle, vision, chemosensation, ion channels, signal transduction, neuropeptide signaling, neurotransmitter synthesis and transport, nicotine tolerance, lipid metabolism, and immunity. This genome sequence, annotation, and analysis provide an important new resource from a well-studied model insect species and will facilitate further biochemical and mechanistic experimental studies of many biological systems in insects.

Published
2016

14. De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals

Author

Tetreau, Guillaume, primary, Sawaya, Michael R., additional, De Zitter, Elke, additional, Andreeva, Elena A., additional, Banneville, Anne-Sophie, additional, Schibrowsky, Natalie, additional, Coquelle, Nicolas, additional, Brewster, Aaron S., additional, Grünbein, Marie Luise, additional, Kovacs, Gabriela Nass, additional, Hunter, Mark S., additional, Kloos, Marco, additional, Sierra, Raymond G., additional, Schiro, Giorgio, additional, Qiao, Pei, additional, Stricker, Myriam, additional, Bideshi, Dennis, additional, Young, Iris D., additional, Zala, Ninon, additional, Engilberge, Sylvain, additional, Gorel, Alexander, additional, Signor, Luca, additional, Teulon, Jean-Marie, additional, Hilpert, Mario, additional, Foucar, Lutz, additional, Bielecki, Johan, additional, Bean, Richard, additional, de Wijn, Raphael, additional, Sato, Tokushi, additional, Kirkwood, Henry, additional, Letrun, Romain, additional, Batyuk, Alexander, additional, Snigireva, Irina, additional, Fenel, Daphna, additional, Schubert, Robin, additional, Canfield, Ethan J., additional, Alba, Mario M., additional, Laporte, Frédéric, additional, Després, Laurence, additional, Bacia, Maria, additional, Roux, Amandine, additional, Chapelle, Christian, additional, Riobé, François, additional, Maury, Olivier, additional, Ling, Wai Li, additional, Boutet, Sébastien, additional, Mancuso, Adrian, additional, Gutsche, Irina, additional, Girard, Eric, additional, Barends, Thomas R. M., additional, Pellequer, Jean-Luc, additional, Park, Hyun-Woo, additional, Laganowsky, Arthur D., additional, Rodriguez, Jose, additional, Burghammer, Manfred, additional, Shoeman, Robert L., additional, Doak, R. Bruce, additional, Weik, Martin, additional, Sauter, Nicholas K., additional, Federici, Brian, additional, Cascio, Duilio, additional, Schlichting, Ilme, additional, and Colletier, Jacques-Philippe, additional

Published
2021
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20. Shock Damage Analysis in Serial Femtosecond Crystallography Data Collected at MHz X-ray Free-Electron Lasers

Author

Gorel, Alexander, primary, Grünbein, Marie, additional, Bean, Richard, additional, Bielecki, Johan, additional, Hilpert, Mario, additional, Cascella, Michele, additional, Colletier, Jacques-Philippe, additional, Fangohr, Hans, additional, Foucar, Lutz, additional, Hartmann, Elisabeth, additional, Hunter, Mark, additional, Kirkwood, Henry, additional, Kloos, Marco, additional, Letrun, Romain, additional, Michelat, Thomas, additional, Shoeman, Robert, additional, Sztuk-Dambietz, Jolanta, additional, Tetreau, Guillaume, additional, Zimmermann, Herbert, additional, Mancuso, Adrian, additional, Barends, Thomas, additional, Doak, R., additional, Stan, Claudiu, additional, and Schlichting, Ilme, additional

Published
2020
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21. Deciphering the molecular mechanisms of mother-to-egg immune protection in the mealworm beetle Tenebrio molitor

Author

Tetreau, Guillaume, primary, Dhinaut, Julien, additional, Galinier, Richard, additional, Audant-Lacour, Pascaline, additional, Voisin, Sébastien N., additional, Arafah, Karim, additional, Chogne, Manon, additional, Hilliou, Frédérique, additional, Bordes, Anaïs, additional, Sabarly, Camille, additional, Chan, Philippe, additional, Walet-Balieu, Marie-Laure, additional, Vaudry, David, additional, Duval, David, additional, Bulet, Philippe, additional, Coustau, Christine, additional, Moret, Yannick, additional, and Gourbal, Benjamin, additional

Published
2020
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22. The C. elegans GATA transcription factor elt-2 mediates distinct transcriptional responses and opposite infection outcomes towards different Bacillus thuringiensis strains

Author

Zárate-Potes, Alejandra, primary, Yang, Wentao, additional, Pees, Barbara, additional, Schalkowski, Rebecca, additional, Segler, Philipp, additional, Andresen, Bentje, additional, Haase, Daniela, additional, Nakad, Rania, additional, Rosenstiel, Philip, additional, Tetreau, Guillaume, additional, Colletier, Jacques-Philippe, additional, Schulenburg, Hinrich, additional, and Dierking, Katja, additional

Published
2020
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25. Identification and expression profile analysis of odorant binding protein and chemosensory protein genes in Bemisia tabaci MED by head transcriptome

Author

Wang, Ran, Li, Fengqi, Zhang, Wei, Zhang, Xiaoman, Qu, Cheng, Tetreau, Guillaume, Sun, Lujuan, Luo, Chen, Zhou, Jingjiang, Gao, Yulin, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Department of Polymer Science and Engineering (USTB), University of Science and Technology Beijing [Beijing] (USTB), Laboratoire d'Ecologie Alpine (LECA ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A and F University, and TETREAU, Guillaume

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

26. Fitness of Bt-resistant cabbage loopers on Bt cotton plants

Author

Tetreau, Guillaume, Wang, Ran, Wang, Ping, TETREAU, Guillaume, Laboratoire d'Ecologie Alpine (LECA ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, and University of Turku

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [SDV.EE.IEO] Life Sciences [q-bio]/Ecology, environment/Symbiosis, ComputingMilieux_MISCELLANEOUS, [SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis
Abstract

International audience

Published
2017

27. Receptors are affected by selection with each Bacillus thuringiensis israelensis Cry toxin but not with the full Bti mixture in Aedes aegypti

Author

Stalinski, Renaud, Laporte, Frederic, Tetreau, Guillaume, Despres, Laurence, Laboratoire d'Ecologie Alpine (LECA ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Laboratoire de Biologie des Populations d'Altitude, Centre National de la Recherche Scientifique (CNRS), and TETREAU, Guillaume

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.EE.IEO] Life Sciences [q-bio]/Ecology, environment/Symbiosis, ComputingMilieux_MISCELLANEOUS, [SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis
Abstract

International audience

Published
2016

28. Larval midgut modifications associated with Bti resistance in the yellow fever mosquito using proteomic and transcriptomic approaches

Author

Tetreau Guillaume, Bayyareddy Krishnareddy, Jones Christopher M, Stalinski Renaud, Riaz Muhammad A, Paris Margot, David Jean-Philippe, Adang Michael J, and Després Laurence

Subjects
Aedes aegypti, Bacillus thuringiensis israelensis, DIGE, Microarray, RT-qPCR, Resistance, Transcriptomics, Proteomics, Midgut, Mosquito, Candidate genes, Biotechnology, TP248.13-248.65, Genetics, QH426-470
Abstract

Abstract Background Bacillus thuringiensis var. israelensis (Bti) is a natural larval mosquito pathogen producing pore-forming toxins targeting the midgut of Diptera larvae. It is used worldwide for mosquito control. Resistance mechanisms of an Aedes aegypti laboratory strain selected for 30 generations with field-collected leaf litter containing Bti toxins were investigated in larval midguts at two levels: 1. gene transcription using DNA microarray and RT-qPCR and 2. differential expression of brush border membrane proteins using DIGE (Differential In Gel Electrophoresis). Results Several Bti Cry toxin receptors including alkaline phosphatases and N-aminopeptidases and toxin-binding V-ATPases exhibited altered expression levels in the resistant strain. The under-expression of putative Bti-receptors is consistent with Bt-resistance mechanisms previously described in Lepidoptera. Four soluble metalloproteinases were found under-transcribed together with a drastic decrease of metalloproteinases activity in the resistant strain, suggesting a role in resistance by decreasing the amount of activated Cry toxins in the larval midgut. Conclusions By combining transcriptomic and proteomic approaches, we detected expression changes at nearly each step of the ingestion-to-infection process, providing a short list of genes and proteins potentially involved in Bti-resistance whose implication needs to be validated. Collectively, these results open the way to further functional analyses to better characterize Bti-resistance mechanisms in mosquitoes.

Published
2012
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30. Additional file 2: of Bacterial microbiota of Aedes aegypti mosquito larvae is altered by intoxication with Bacillus thuringiensis israelensis

Author

Tetreau, Guillaume, Grizard, Stéphanie, Chandrashekhar Patil, Florence-Hélène Tran, Van, Van Tran, Stalinski, Renaud, Laporte, Frédéric, Mavingui, Patrick, Després, Laurence, and Moro, Claire Valiente

Subjects
fungi
Abstract

Figure S2. Composite picture of a DGGE gel containing all four groups of larvae. Bands excised and sequenced are indicated by a red rectangle. The corresponding species identified are indicated in the table. (PDF 242 kb)

Published
2018
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31. Additional file 3: of Bacterial microbiota of Aedes aegypti mosquito larvae is altered by intoxication with Bacillus thuringiensis israelensis

Author

Tetreau, Guillaume, Grizard, Stéphanie, Chandrashekhar Patil, Florence-Hélène Tran, Van, Van Tran, Stalinski, Renaud, Laporte, Frédéric, Mavingui, Patrick, Després, Laurence, and Moro, Claire Valiente

Subjects
human activities
Abstract

Table S1. Tukey’s post-hoc test outputs from larval bacterial microbiota. Linear models were used to test the relationship between alpha-diversity metrics (diversity) and treatment groups (treatment). Tukey’s post-hoc tests were used to compare pair of treatment (Z-tests). P-values are then reported for each model and indicated in bold when significant (P < 0.05). (PDF 195 kb)

Published
2018
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32. Additional file 4: of Bacterial microbiota of Aedes aegypti mosquito larvae is altered by intoxication with Bacillus thuringiensis israelensis

Author

Tetreau, Guillaume, Grizard, Stéphanie, Chandrashekhar Patil, Florence-Hélène Tran, Van, Van Tran, Stalinski, Renaud, Laporte, Frédéric, Mavingui, Patrick, Després, Laurence, and Moro, Claire Valiente

Abstract

Table S2. Band counting numbers, Shannon index (diversity), Simpson index (diversity) and Pielou’s index (evenness). They are based on DGGE band-matching surfaces. Results are for larvae never exposed to Bacillus thuringiensis israelensis (Bti) (“Control”) and for larvae exposed to Bti, including larvae dead in less than 6 h of exposure to Bti (“Susceptible”), in between 6 and 11 h (“Intermediate”), and past 11 h (“Tolerant”). Results are given with mean (± standard deviation) (minimum-maximum values). (PDF 277 kb)

Published
2018
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33. Additional file 1: of Bacterial microbiota of Aedes aegypti mosquito larvae is altered by intoxication with Bacillus thuringiensis israelensis

Author

Tetreau, Guillaume, Grizard, Stéphanie, Chandrashekhar Patil, Florence-Hélène Tran, Van, Van Tran, Stalinski, Renaud, Laporte, Frédéric, Mavingui, Patrick, Després, Laurence, and Moro, Claire Valiente

Abstract

Figure S1. Picture of the DGGE gel containing unexposed larvae sampled at 0, 5, 10 and 24 h. (PDF 108 kb)

Published
2018
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34. Additional file 7: of Bacterial microbiota of Aedes aegypti mosquito larvae is altered by intoxication with Bacillus thuringiensis israelensis

Author

Tetreau, Guillaume, Grizard, Stéphanie, Chandrashekhar Patil, Florence-Hélène Tran, Van, Van Tran, Stalinski, Renaud, Laporte, Frédéric, Mavingui, Patrick, Després, Laurence, and Moro, Claire Valiente

Abstract

Figure S4. Non-metric multi-dimensional scaling plots of the bacterial communities of larvae based on DGGE lane analysis. 3D stress of the NMDS was 0.14. The NMDS representation is based on a Bray-Curtis dissimilarity matrix. Correspondence of sample names is indicated in the legend of Fig. 2. (PDF 65 kb)

Published
2018
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39. Candidate genes revealed by a genome scan for mosquito resistance to a bacterial insecticide: sequence and gene expression variations

Author

David Jean-Philippe, Tetreau Guillaume, Paris Margot, Bonin Aurélie, and Després Laurence

Subjects
Biotechnology, TP248.13-248.65, Genetics, QH426-470
Abstract

Abstract Background Genome scans are becoming an increasingly popular approach to study the genetic basis of adaptation and speciation, but on their own, they are often helpless at identifying the specific gene(s) or mutation(s) targeted by selection. This shortcoming is hopefully bound to disappear in the near future, thanks to the wealth of new genomic resources that are currently being developed for many species. In this article, we provide a foretaste of this exciting new era by conducting a genome scan in the mosquito Aedes aegypti with the aim to look for candidate genes involved in resistance to Bacillus thuringiensis subsp. israelensis (Bti) insecticidal toxins. Results The genome of a Bti-resistant and a Bti-susceptible strains was surveyed using about 500 MITE-based molecular markers, and the loci showing the highest inter-strain genetic differentiation were sequenced and mapped on the Aedes aegypti genome sequence. Several good candidate genes for Bti-resistance were identified in the vicinity of these highly differentiated markers. Two of them, coding for a cadherin and a leucine aminopeptidase, were further examined at the sequence and gene expression levels. In the resistant strain, the cadherin gene displayed patterns of nucleotide polymorphisms consistent with the action of positive selection (e.g. an excess of high compared to intermediate frequency mutations), as well as a significant under-expression compared to the susceptible strain. Conclusion Both sequence and gene expression analyses agree to suggest a role for positive selection in the evolution of this cadherin gene in the resistant strain. However, it is unlikely that resistance to Bti is conferred by this gene alone, and further investigation will be needed to characterize other genes significantly associated with Bti resistance in Ae. aegypti. Beyond these results, this article illustrates how genome scans can build on the body of new genomic information (here, full genome sequence and MITE characterization) to finally hold their promises and help pinpoint candidate genes for adaptation and speciation.

Published
2009
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40. A MITE-based genotyping method to reveal hundreds of DNA polymorphisms in an animal genome after a few generations of artificial selection

Author

Tetreau Guillaume, Després Laurence, Paris Margot, Bonin Aurélie, David Jean-Philippe, and Kilian Andrzej

Subjects
Biotechnology, TP248.13-248.65, Genetics, QH426-470
Abstract

Abstract Background For most organisms, developing hundreds of genetic markers spanning the whole genome still requires excessive if not unrealistic efforts. In this context, there is an obvious need for methodologies allowing the low-cost, fast and high-throughput genotyping of virtually any species, such as the Diversity Arrays Technology (DArT). One of the crucial steps of the DArT technique is the genome complexity reduction, which allows obtaining a genomic representation characteristic of the studied DNA sample and necessary for subsequent genotyping. In this article, using the mosquito Aedes aegypti as a study model, we describe a new genome complexity reduction method taking advantage of the abundance of miniature inverted repeat transposable elements (MITEs) in the genome of this species. Results Ae. aegypti genomic representations were produced following a two-step procedure: (1) restriction digestion of the genomic DNA and simultaneous ligation of a specific adaptor to compatible ends, and (2) amplification of restriction fragments containing a particular MITE element called Pony using two primers, one annealing to the adaptor sequence and one annealing to a conserved sequence motif of the Pony element. Using this protocol, we constructed a library comprising more than 6,000 DArT clones, of which at least 5.70% were highly reliable polymorphic markers for two closely related mosquito strains separated by only a few generations of artificial selection. Within this dataset, linkage disequilibrium was low, and marker redundancy was evaluated at 2.86% only. Most of the detected genetic variability was observed between the two studied mosquito strains, but individuals of the same strain could still be clearly distinguished. Conclusion The new complexity reduction method was particularly efficient to reveal genetic polymorphisms in Ae. egypti. Overall, our results testify of the flexibility of the DArT genotyping technique and open new prospects as regards its application to a wider range of species, including animals which have been refractory to it so far. DArT has also a role to play in the current burst of whole-genome scans carried out in various organisms, which track signatures of selection in order to unravel the basis of genetic adaptation.

Published
2008
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41. Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta

Author

Kanost, Michael, Arrese, Estela, Cao, Xiaolong, Chen, Yun-Ru, Chellapilla, Sanjay, Goldsmith, Marian, Grosse-Wilde, Ewald, Heckel, David, Herndon, Nicolae, Jiang, Haobo, Papanicolaou, Alexie, Qu, Jiaxin, Soulages, Jose, Vogel, Heiko, Walters, James, Waterhouse, Robert, Ahn, Seung-Joon, Almeida, Francisca, An, Chunju, Aqrawi, Peshtewani, An, Anne, Bryant, William, Bucks, Sascha, Chao, Hsu, Chevignon, Germain, Christen, Jayne, Clarke, David, Dittmer, Neal, Ferguson, Laura C.F., Garavelou, Spyridoula, Gordon, Karl H.J., Gunaratna, Ramesh, Han, Yi, Hauser, Frank, He, Yan, Hirsh, Ariana, Hu, Yi, Jiang, Hongbo, Kalra, Divya, Klinner, Christian, König, Christopher, Kovar, Christie, Kroll, Ashley, Kuwar, Suyog, Lee, Sandy, Lehman, Rüdiger, Li, Kai, Li, Zhaofei, Han, Hanquan, Lovelace, Shanna, Lu, Zhiqiang, Mansfield, Jennifer, Mcculloch, Kyle, Mathew, Tittu, Morton, Brian, Muzny, Donna, Neunemann, David, Ongeri, Fiona, Pauchet, Yan, Li, Ling-Ling, Pyrousis, Ioannis, Rao, Xiang-Jun, Redding, Amanda, Roesel, Charles, Sanchez-Gracia, Alejandro, Schaack, Sarah, Shukla, Aditi, Tetreau, Guillaume, Wang, Yan, Hu, Guang-Hua, Traut, Walther, Walsh, Tom, Worley, Kim, Wu, Di, Wu, Wenbi, Wu, Yuan-Qing, Zhang, Xiufeng, Zou, Zhen, Han, Hanna, Briscoe, Adriana, Burmester, Thorsten, Clem, Rollie, Feyereisen, René, Grimmelikhuijzen, Cornelis J.P., Hamodrakas, Stavros, Han, Bill, Hu, Elisabeth, Jermiin, Lars, Qu, Que, He, Herman, Lorenzen, Marce, Han, Hans, Michalopoulos, Ioannis, Morton, David, Muthukrishnan, Subbaratnam, Oakeshott, John, Palmer, Will, Park, Yoonseong, Passarelli, A. Lorena, Rozas, Julio, Schwartz, Lawrence, Smith, Wendy, Southgate, Agnes, An, Andreas, Vogt, Richard, Wang, Ping, Werren, John, Yu, Xiao-Qiang, Jiang, Jing-Jiang, Brown, Susan, Scherer, Steven, Richards, Richard, Blissard, Gary, National Tsing Hua University [Hsinchu] (NTHU), University of Rhode Island (URI), Max Planck Institute for Chemical Ecology, Max-Planck-Gesellschaft, Dpt of Entomology and Plant Pathology, Oklahoma State University [Stillwater], Ecosystem Sciences, Commonwealth Scientific and Industrial Research Organisation [Canberra] (CSIRO), MRC Centre for Neuropsychiatric Genetics and Genomics, Medical Research Council-Cardiff University, Université de Lausanne (UNIL), Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), University of Melbourne, Institut de biotechnologie des plantes (IBP), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Huazhong University of Science and Technology [Wuhan] (HUST), Laboratoire des sciences de l'ingénieur, de l'informatique et de l'imagerie (ICube), Institut National des Sciences Appliquées - Strasbourg (INSA Strasbourg), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-École Nationale du Génie de l'Eau et de l'Environnement de Strasbourg (ENGEES)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), University of North Carolina [Chapel Hill] (UNC), University of North Carolina System (UNC), Human Genome Sequencing Center, Baylor College of Medicine, Baylor College of Medicine (BCM), Baylor University-Baylor University, Laboratoire d'Ecologie Alpine (LECA ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Laboratoire Interdisciplinaire Carnot de Bourgogne [Dijon] (LICB), Université de Bourgogne (UB)-Université de Technologie de Belfort-Montbeliard (UTBM)-Centre National de la Recherche Scientifique (CNRS), Human Genome Sequencing Center [Houston] (HGSC), Laboratoire de Recherche en Informatique et ses Applications de Vannes et Lorient (VALORIA), Université de Bretagne Sud (UBS), Régulation des gènes et signalisation cellulaire, Institut National de la Santé et de la Recherche Médicale (INSERM), Biocenter Grindel, Zoological Museum-University of Hamburg, Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), Biophysics and Bioinformatics Laboratory, Northwestern University [Evanston], Department of Entomology, Michigan State University [East Lansing], Michigan State University System-Michigan State University System, Departament de Genètica and Institut de Recerca de la Biodiversitat, Universitat de Barcelona (UB), Laboratoire d'informatique de l'École polytechnique [Palaiseau] (LIX), École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS), University of Turku, Nottingham University Business School, The Hospital for sick children [Toronto] (SickKids), Université de Tours-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Matériaux et nanosciences d'Alsace, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire d'Ecologie Alpine (LECA), Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Joseph Fourier - Grenoble 1 (UJF)-Université Grenoble Alpes (UGA), Université de Technologie de Belfort-Montbeliard (UTBM)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Department of Industrial Engineering and Management Sciences, Northwestern University, Centre National de la Recherche Scientifique (CNRS)-École polytechnique (X), TETREAU, Guillaume, Oklahoma State University [Stillwater] (OSU), Université de Lausanne = University of Lausanne (UNIL), École Nationale du Génie de l'Eau et de l'Environnement de Strasbourg (ENGEES)-Université de Strasbourg (UNISTRA)-Institut National des Sciences Appliquées - Strasbourg (INSA Strasbourg), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de Recherche en Informatique et en Automatique (Inria)-Les Hôpitaux Universitaires de Strasbourg (HUS)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Department of Entomology and Plant Pathology [Oklahoma State University], Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), and Nottingham University Business School [Nottingham]

Subjects
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

42. Bacterial microbiota of Aedes aegypti mosquito larvae is altered by intoxication with Bacillus thuringiensis israelensis

Author

Tetreau, Guillaume, Grizard, Stéphanie, Patil, Chandrashekhar, Tran, Florence-Hélène, Tran Van, Van, Stalinski, Renaud, Laporte, Frédéric, Mavingui, Patrick, Despres, Laurence, Valiente Moro, Claire, Laboratoire d'Ecologie Alpine (LECA ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Centre de recherches insulaires et observatoire de l'environnement (CRIOBE), Université de Perpignan Via Domitia (UPVD)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Service de Réanimation Médicale, CHU Bordeaux [Bordeaux]-Groupe hospitalier Pellegrin, Laboratoire de Biologie des Populations d'Altitude, Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ecologie Alpine (LECA), Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Joseph Fourier - Grenoble 1 (UJF)-Université Grenoble Alpes (UGA), Université de Perpignan Via Domitia (UPVD)-École pratique des hautes études (EPHE)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), ERA-NET BiodivERsA, DFG KL2087/6-1, ANR-13-EBID-0007-01, and FWFI-1437

Subjects
moustique, Mosquito Control, [SDV]Life Sciences [q-bio], Bacillus thuringiensis, [SDV.BID]Life Sciences [q-bio]/Biodiversity, Holobiont, lcsh:Infectious and parasitic diseases, microbiote, aedes aegypti, intoxication, Aedes, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Larval microbiota, bcterial community fingerprinting, Bacterial community fingerprinting, Animals, lcsh:RC109-216, Pest Control, Biological, ComputingMilieux_MISCELLANEOUS, Gram-Positive Bacterial Infections, Denaturing gradient gel electrophoresis (DGGE), diptera, bacillus thuringiensis, denaturing gradient gel electrophoresis (DGGE), larval microbiota, holobiont, Denaturing Gradient Gel Electrophoresis, Microbiota, Research, Diptera, Microbiology and Parasitology, fungi, DNA Fingerprinting, Microbiologie et Parasitologie, larve, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Larva, bacillus thuringiensis var. israelensis, [SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis
Abstract

Background Insect microbiota is a dynamic microbial community that can actively participate in defense against pathogens. Bacillus thuringiensis (Bt) is a natural entomopathogen widely used as a bioinsecticide for pest control. Although Bt’s mode of action has been extensively studied, whether the presence of microbiota is mandatory for Bt to effectively kill the insect is still under debate. An association between a higher tolerance and a modified microbiota was already evidenced but a critical point remained to be solved: is the modified microbiota a cause or a consequence of a higher tolerance to Bt? Methods In this study we focused on the mosquito species Aedes aegypti, as no work has been performed on Diptera on this topic to date, and on B. thuringiensis israelensis (Bti), which is used worldwide for mosquito control. To avoid using antibiotics to cure bacterial microbiota, mosquito larvae were exposed to an hourly increasing dose of Bti during 25 hours to separate the most susceptible larvae dying quickly from more tolerant individuals, with longer survival. Results Denaturing gradient gel electrophoresis (DGGE) fingerprinting revealed that mosquito larval bacterial microbiota was strongly affected by Bti infection after only a few hours of exposure. Bacterial microbiota from the most tolerant larvae showed the lowest diversity but the highest inter-individual differences. The proportion of Bti in the host tissue was reduced in the most tolerant larvae as compared to the most susceptible ones, suggesting an active control of Bti infection by the host. Conclusions Here we show that a modified microbiota is associated with a higher tolerance of mosquitoes to Bti, but that it is rather a consequence of Bti infection than the cause of the higher tolerance. This study paves the way to future investigations aiming at unraveling the role of host immunity, inter-species bacterial competition and kinetics of host colonization by Bti that could be at the basis of the phenotype observed in this study. Electronic supplementary material The online version of this article (10.1186/s13071-018-2741-8) contains supplementary material, which is available to authorized users.

Published
2017

43. Specific Pathogen Recognition by Multiple Innate Immune Sensors in an Invertebrate

Author

Tetreau, Guillaume, Pinaud, Silvain, Portet, Anaïs, Galinier, Richard, Gourbal, Benjamin, Duval, David, Interactions Hôtes-Pathogènes-Environnements (IHPE), and Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Perpignan Via Domitia (UPVD)

Subjects
lcsh:Immunologic diseases. Allergy, pattern-recognition receptor, Immunology, immune specificity, proteomic profiling, invertebrate innate immunity, interactome, pathogen sensing, lcsh:RC581-607, hemocyte-free hemolymph, Biomphalaria glabrata, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, Original Research
Abstract

International audience; Detection of pathogens by all living organisms is the primary step needed to implement a coherent and efficient immune response. This implies a mediation by different soluble and/or membrane-anchored proteins related to innate immune receptors called PRRs (pattern-recognition receptors) to trigger immune signaling pathways. In most invertebrates , their roles have been inferred by analogy to those already characterized in vertebrate homologs. Despite the induction of their gene expression upon challenge and the presence of structural domains associated with the detection of pathogen-associated molecular patterns in their sequence, their exact role in the induction of immune response and their binding capacity still remain to be demonstrated. To this purpose, we developed a fast interactome approach, usable on any host-pathogen couple, to identify soluble proteins capable of directly or indirectly detecting the presence of pathogens. To investigate the molecular basis of immune recognition specificity, different pathogens (Gram-positive bacterium, Micrococcus luteus; Gram-negative, Escherichia coli; yeast, Saccharomyces cerevisiae; and metazoan parasites, Echinostoma caproni or Schistosoma mansoni) were exposed to hemocyte-free hemolymph from the gastro-pod Biomphalaria glabrata. Twenty-three different proteins bound to pathogens were identified and grouped into three different categories based on their primary function. Each pathogen was recognized by a specific but overlapping set of circulating proteins in mollusk's hemolymph. While known PRRs such as C-type lectins were identified, other proteins not known to be primarily involved in pathogen recognition were found, including actin, tubulin, collagen, and hemoglobin. Confocal microscopy and specific fluorescent labeling revealed that extracellular actin present in snail hemolymph was able to bind to yeasts and induce their clotting, a preliminary step for their elimination by the snail immune system. Aerolysin-like proteins (named biomphalysins) were the only ones involved in the recognition of all the five pathogens tested, suggesting a sentinel role of these horizontally acquired toxins. These findings highlight the diversity and complexity of a highly specific innate immune sensing system. It paves the way for the use of such approach on a wide range of host-pathogen systems to provide new insights into the specificity and diversity of immune recognition by innate immune systems.

Published
2017

45. Pre-selecting resistance against individual Bti Cry toxins facilitates the development of resistance to the Bti toxins cocktail

Author

Stalinski, Renaud, Tetreau, Guillaume, Gaude, Thierry, Despres, Laurence, Laboratoire d'Ecologie Alpine (LECA), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Reproduction et développement des plantes (RDP), École normale supérieure - Lyon (ENS Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie des Populations d'Altitude, Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), TETREAU, Guillaume, and Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Joseph Fourier - Grenoble 1 (UJF)-Université Grenoble Alpes (UGA)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [SDV.EE.IEO] Life Sciences [q-bio]/Ecology, environment/Symbiosis, ComputingMilieux_MISCELLANEOUS, [SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis
Abstract

International audience

Published
2014

50. The Cultivation of Bt Corn Producing Cry1Ac Toxins Does Not Adversely Affect Non-Target Arthropods

Author

Guo, Yanyan, Feng, Yanjie, Ge, Yang, Tetreau, Guillaume, Chen, Xiaowen, Dong, Xuehui, Shi, Wangpeng, Desneux, Nicolas, School of Information, Renmin University of China, Beijing, Laboratoire d'Ecologie Alpine (LECA), Centre National de la Recherche Scientifique (CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Université Joseph Fourier - Grenoble 1 (UJF)-Université Grenoble Alpes (UGA), Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV.EE.ECO]Life Sciences [q-bio]/Ecology, environment/Ecosystems, [SDE]Environmental Sciences, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2014

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