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12 results on '"Thiruvengadam, Girija"'

1. Differential regulation of MYC expression by PKHD1/Pkhd1 in human and mouse kidneys: phenotypic implications for recessive polycystic kidney disease

Author

Harafuji, Naoe, primary, Yang, Chaozhe, additional, Wu, Maoqing, additional, Thiruvengadam, Girija, additional, Gordish-Dressman, Heather, additional, Thompson, R. Griffin, additional, Bell, P. Darwin, additional, Rosenberg, Avi Z., additional, Dafinger, Claudia, additional, Liebau, Max C., additional, Bebok, Zsuzsanna, additional, Caldovic, Ljubica, additional, and Guay-Woodford, Lisa M., additional

Published
2023
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5. IFN Stimulates ACE2 Expression in Pediatric Airway Epithelial Cells

Author

Salka, Kyle, primary, Abutaleb, Karima, additional, Chorvinsky, Elizabeth, additional, Thiruvengadam, Girija, additional, Arroyo, Maria, additional, Gomez, Jose L., additional, Gutierrez, Maria J., additional, Pillai, Dinesh K., additional, Jaiswal, Jyoti K., additional, and Nino, Gustavo, additional

Published
2021
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6. A single-nucleotide polymorphism in a Plasmodium berghei ApiAP2 transcription factor alters the development of host immunity

Author

Akkaya, Munir, Bansal, Abhisheka, Sheehan, Patrick W., Pena, Mirna, Molina-Cruz, Alvaro, Orchard, Lindsey M., Cimperman, Clare K., Qi, Chen-Feng, Ross, Philipp, Yazew, Takele, Sturdevant, Daniel, Anzick, Sarah L., Thiruvengadam, Girija, Otto, Thomas Dan, Billker, Oliver, Llinas, Manuel, Miller, Louis H., Pierce, Susan K., Akkaya, Munir, Bansal, Abhisheka, Sheehan, Patrick W., Pena, Mirna, Molina-Cruz, Alvaro, Orchard, Lindsey M., Cimperman, Clare K., Qi, Chen-Feng, Ross, Philipp, Yazew, Takele, Sturdevant, Daniel, Anzick, Sarah L., Thiruvengadam, Girija, Otto, Thomas Dan, Billker, Oliver, Llinas, Manuel, Miller, Louis H., and Pierce, Susan K.

Abstract

The acquisition of malaria immunity is both remarkably slow and unpredictable. At present, we know little about the malaria parasite genes that influence the host's ability to mount a protective immune response. Here, we show that a single-nucleotide polymorphism (SNP) resulting in a single amino acid change (S to F) in an ApiAP2 transcription factor in the rodent malaria parasite Plasmodium berghei (Pb) NK65 allowed infected mice to mount a T helper cell 1 (T(H)1)-type immune response that controlled subsequent infections. As compared to PbNK65(S), PbNK65(F) parasites differentially expressed 46 genes, most of which are predicted to play roles in immune evasion. PbNK65(F) infections resulted in an early interferon-gamma response and a later expansion of germinal centers, resulting in high levels of infected red blood cell-specific T(H)1-type immunoglobulin G2b (IgG2b) and IgG2c antibodies. Thus, the Pb ApiAP2 transcription factor functions as a critical parasite virulence factor in malaria infections.

Published
2020
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7. A single-nucleotide polymorphism in a Plasmodium berghei ApiAP2 transcription factor alters the development of host immunity

Author

Akkaya, Munir, primary, Bansal, Abhisheka, additional, Sheehan, Patrick W., additional, Pena, Mirna, additional, Molina-Cruz, Alvaro, additional, Orchard, Lindsey M., additional, Cimperman, Clare K., additional, Qi, Chen-Feng, additional, Ross, Philipp, additional, Yazew, Takele, additional, Sturdevant, Daniel, additional, Anzick, Sarah L., additional, Thiruvengadam, Girija, additional, Otto, Thomas Dan, additional, Billker, Oliver, additional, Llinás, Manuel, additional, Miller, Louis H., additional, and Pierce, Susan K., additional

Published
2020
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8. Anoctamin 5 Knockout Mouse Model Recapitulates LGMD2L Muscle Pathology and Offers Insight Into in vivoFunctional Deficits

Author

Thiruvengadam, Girija, Sreetama, Sen Chandra, Charton, Karine, Hogarth, Marshall, Novak, James S., Suel-Petat, Laurence, Chandra, Goutam, Allard, Bruno, Richard, Isabelle, and Jaiswal, Jyoti K.

Abstract

Mutations in the Anoctamin 5 (Ano5) gene that result in the lack of expression or function of ANO5 protein, cause Limb Girdle Muscular Dystrophy (LGMD) 2L/R12, and Miyoshi Muscular Dystrophy (MMD3). However, the dystrophic phenotype observed in patient muscles is not uniformly recapitulated by ANO5 knockout in animal models of LGMD2L. Here we describe the generation of a mouse model of LGMD2L generated by targeted out-of-frame deletion of the Ano5gene. This model shows progressive muscle loss, increased muscle weakness, and persistent bouts of myofiber regeneration without chronic muscle inflammation, which recapitulates the mild to moderate skeletal muscle dystrophy reported in the LGMD2L patients. We show that these features of ANO5 deficient muscle are not associated with a change in the calcium-activated sarcolemmal chloride channel activity or compromised in vivoregenerative myogenesis. Use of this mouse model allows conducting in vivoinvestigations into the functional role of ANO5 in muscle health and for preclinical therapeutic development for LGMD2L.

Published
2021
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9. Immunogenic and functional characterization of Toxoplasma gondii surface antigens (SAG1 and SAG2)

Author

Thiruvengadam, Girija

Subjects
animal diseases, parasitic diseases, Uncategorized
Abstract

Toxoplasmosis is a zoonotic infection caused by the protozoan parasite Toxoplasma gondii. Among the different forms involved in the parasite’s life cycle, tachyzoites are the most infectious and known to infect a wide variety of tissues to produce inflammatory responses. Two of the most abundant cell surface proteins on the tachyzoite surface, SAG1 and SAG2, are known to play important role during the invasion of host cells. In order to explore the potential of using SAG1 and SAG2 antigens in diagnosis and vaccine development, a recombinant DNA for a chimeric SAG1/2 gene was constructed and the fusion protein was produced in the yeast Pichia pastoris. The sensitivity and specificity of the fusion protein as an antigen were confirmed through western blotting using serum from toxoplasmosis positive individuals. The immunogenicity of the recombinant protein was seen in immunized mice, where specific antibodies were detected. Splenocytes from immunized mice readily proliferate and secrete large amount of IFN-γ when stimulated with the total lysate antigen of tachyzoite, suggesting that a T helper 1 type immune response was induced. Immunized mice have a higher survival rate when challenged with virulent tachyzoites (RH strain) compared to control (non-protein immunized) mice. This suggests that the chimeric protein is potentially a good candidate for diagnostic and vaccine development against T. gondii. Since SAG1 and SAG2 are known to play a role in tachyzoite invasion, the effect of silencing these two genes using RNA interference on host tissue invasion was examined. The siRNA molecules were able to downregulate the expression of both SAG1 and SAG2 in tachyzoites when compared to controls. However, the silencing of SAG1 and SAG2 appears to have little effect on the invasion of host cells by tachyzoites. This suggests that host cell invasion by tachyzoites may not depend on SAG1 and SAG2 alone but other surface antigens and/or secreted proteins might be involved.

Published
2017
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12. Immunogenic characterization of the chimeric surface antigen 1 and 2 (SAG1/2) of Toxoplasma gondii expressed in the yeast Pichia pastoris.

Author

Yee Ling Lau, Thiruvengadam, Girija, Wei Wei Lee, and Mun Yik Fong

Subjects
*TOXOPLASMA gondii, *PICHIA pastoris, *SERUM, *WESTERN immunoblotting, *RECOMBINANT proteins, *TOXOPLASMOSIS, *CYTOKINES, *LABORATORY mice
Abstract

In this study, we successfully expressed a chimerical surface antigen 1 and 2 (SAG1/2) of Toxoplasma gondii in Pichia pastoris. Eighty human serum samples, including 60 from confirmed cases of toxoplasmosis, were tested against the purified recombinant SAG1/2 in Western blots. Results of Western blots targeted at Toxoplasma IgG and IgM showed that the recombinant SAG1/2 reacted with all sera from the toxoplasmosis cases but none with the Toxoplasma-negative serum samples. These results showed that the P. pastoris-derived recombinant SAG1/2 was sensitive and specific and suitable for use as antigen for detecting anti- Toxoplasma antibodies. To further investigate the immunological characteristic of the recombinant protein, the recombinant SAG1/2 was injected subcutaneously into BALB/c mice, and their serum was tested against total protein lysate of T. gondii. Mice immunized with the recombinant SAG1/2 reacted specifically with the native SAG1 and SAG2 of T. gondii. Significant proliferation of splenocytes stimulated with tachyzoite total protein lysate was observed in vaccinated BALB/c mice but not in those from negative control mice. Specific production of IFN-γ, the Th1-type cytokines, was also found in stimulated splenocytes from vaccinated mice. These results show that the chimeric protein recombinant SAG1/2 can elicit a Th1-associated protection against T. gondii infections in mice. Finally, vaccinated mice were significantly protected against lethal challenge with live T. gondii RH strain tachyzoites ( P < 0.005), and their survival time increased significantly compared to the negative control. [ABSTRACT FROM AUTHOR]

Published
2011
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