1. Populations structure of the malaria vector Anopheles funestus in Senegal based on microsatellite and cytogenetic data
- Author
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Ibrahima Dia, Anna Cohuet, Frédéric Simard, Michel Raymond, Didier Fontenille, Laboratoire de Lutte contre des Insectes Nuisibles (LIN-IRD), Institut Recherche Développement, Institut Pasteur de Dakar, Réseau International des Instituts Pasteur (RIIP), Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale (OCEAC), Institut des Sciences de l'Evolution de Montpellier (UMR ISEM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Montpellier (UM)-Institut de recherche pour le développement [IRD] : UR226-Centre National de la Recherche Scientifique (CNRS), and This study was funded by the French ministry of research throughout the PAL + project. We thank F. Rousset for helpful comments.
- Subjects
Ecological selection ,MESH: Geography ,CHROMOSOME ,[SDV]Life Sciences [q-bio] ,MESH: Chromosomes/genetics ,STRUCTURE DE POPULATION ,Genome ,Linkage Disequilibrium ,0302 clinical medicine ,Gene Frequency ,MESH: Chromosome Inversion ,INVERSION ,MESH: Animals ,MESH: Microsatellite Repeats/genetics ,Genetics ,0303 health sciences ,Geography ,MESH: Genetic Variation ,Anopheles funestus ,MESH: Genetics, Population ,Incipient speciation ,Senegal ,3. Good health ,MESH: Anopheles/genetics ,MESH: Linkage Disequilibrium ,MOUSTIQUE ,Microsatellite ,MICROSATELLITE ,030231 tropical medicine ,Biology ,Chromosomes ,03 medical and health sciences ,MESH: Senegal ,Genetic algorithm ,Anopheles ,parasitic diseases ,medicine ,MESH: Gene Frequency ,Animals ,Molecular Biology ,030304 developmental biology ,Isolation by distance ,VECTEUR ,Genetic Variation ,PALUDISME ,DIVERSITE GENETIQUE ,medicine.disease ,Genetics, Population ,Insect Science ,Chromosome Inversion ,POLYMORPHISME GENETIQUE ,Malaria ,Microsatellite Repeats - Abstract
The study of chromosomal inversions distribution within natural Anopheles funestus populations from West Africa revealed high levels of genetic structuring. In Burkina Faso, this was interpreted as evidence for incipient speciation, and two chromosomal forms were described, namely ‘Folonzo' and ‘Kiribina'. Assignation of field collected specimens to one chromosomal form depends upon application of an algorithm based on chromosomal inversions. We assessed relevance and applicability of this algorithm on An. funestus populations from Senegal, where both forms occur. Furthermore, we estimated the level of genetic differentiation between populations using microsatellite loci spread over the whole genome. Significant genetic differentiation was revealed between geographical populations of An. funestus , and the pattern observed suggested isolation by distance. Chromosomal heterogeneity was not detected by microsatellite markers. Thus, although incipient speciation could not be ruled out by our data, our results suggest that differential environmental selection pressure acting on inversions should be considered a major factor in shaping their distribution in wild An. funestus populations.
- Published
- 2004
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