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Neophyllachora religiosa Literatus, Haituk & Cheew., sp. nov. Index Fungorum number: IF900393 Etymology:— The epithet “ religiosa ” refers to the epithet name of the host plant “ Ficus religiosa ”, from which the holotype was collected. Holotype:— MFLU 23-0258 Associated with living leaves of Ficus religiosa. Pseudostromata elongated, irregular, discrete, sparse, coalescent, glabrous, shiny, intraepidermal to subepidermal, epiphyllous, multilocular, occasionally amphigenous, often or rarely covering the leaf surface. Sexual morph: Ascomata 83–172 × 127–349 µm (x̄ = 127.5 × 238 µm, n = 20), perithecial, globose to subglobose, solitary or aggregated, ostiolate. Ostiole conspicuous. Peridium 21–30 µm thick, dark brown to black, lateral part wider than basal part, compactly arranged strongly melanized cells. Paraphyses 1–2 µm wide, filiform, numerous, persistent, septate, unbranched, longer than asci. Asci 59–140 × 14–25 µm (x̄ = 88 × 20 µm, n = 30), 8-spored, J-, unitunicate, persistent, cylindrical to fusiform, short-pedicellate, walls uniform in thickness but not specially thickened at the apex and without visible apical structures. Ascospores 8–12 × 5–9 µm (x̄ = 13 × 7 µm, n = 30), uniseriate to biseriate, overlapping, hyaline to olivaceous, globose to elliptical, 1-2 guttules, with a central concave depression, covered by a mucilaginous sheath, irregularly thickened sheath, 1–4.5 µm thickness, widely thickened at the lateral. Asexual morph: Spermacial or Conidial. Conidiomata bearing conidiogenous cells. Conidiogenous cells holoblastic. Conidia 3.5–6 × 2–4 µm (x̄ = 4.75 × 3 µm, n = 30), ellipsoidal, hyaline, rarely septate. Spermatial locules ampulliform. Spermatiogenous cells produce spermatia. Spermatia 7–16 × 0.9–1.4 µm (x̄ = 11.5 × 1.15 µm, n = 30), botuliform or falciform, narrowly rounded at both ends, curved, aseptate, hyaline, smooth-walled. Material examined— THAILAND, Chiang Mai Province, on living leaves of Ficus religiosa (Moraceae), (19 December 2022 (MFLU 23-0252), 18 November 2022 (MFLU 23-0253), 1 December 2022 (MFLU 23-0254), 22 October 2022 (MFLU 23-0255), 27 November 2022 (MFLU 23-0256), 3 September 2022 (MFLU 23-0257), 6 September 2022 (MFLU 23-0258)), Irish C. E. Literatus & Ratchadawan Cheewangkoon (MFLU 23-0258; Holotype, CRC-H196; Isotype) (Fig. 3, 4, 5). GenBank Accession Numbers for ITS — MFLU 23-0252; OQ821004, MFLU 23-0253; OQ821005, MFLU 23- 0254; OQ821006, MFLU 23-0255; OQ821007, MFLU 23-0256; OQ821008, MFLU 23-0257; OQ821009, MFLU 23-0258; OQ821010. Notes — The fungus formed solitary to gregarious black spots, mainly on the upper surface of the host. The new species shows a close phylogenetic association with N. fici, and both species are reported in the same host genus (Ficus) but in different localities (Thailand vs. Taiwan). Morphologically, N. religiosa differs from N. fici in the dimensions of asci (55–185 × 11–26 vs. 90–100 × 15–19 µm) and the thickness of the peridium (15–40 vs. 20–25 µm), and further differs in the ascospore characteristics. The new species possesses ascospores with guttules and are covered with thick gelatinous sheath, whereas N. fici lacks sheath in the ascospores. N. religiosa differs from the type N. myrciae in the dimension of pseudostromata (2–3 vs. 3–6 µm), thinner paraphyses (1.5–2.5 vs. 2.5–4.5 µm), shape of asci (fusoid vs. cylindrical to fusiform), shape of ascospores (lunate vs. globose to elliptical), presence of sheath in the ascospores, arrangement of ascospores (1-2 seriate vs. biseriate to multiseriate), color of ascospores (hyaline to olivaceous vs. hyaline), host (Ficus religiosa vs. Myrcia sp.) and the distribution (Thailand vs. Brazil) but shares similar characteristics in the size of ascomata, asci and coelomycetes asexual state. N. religiosa shares similar ascospores characteristics to N. cerradensis in elliptic-oblong shape ascospores with gelatinous sheath. However, N. religiosa differs in the size of ascospores (8–15 × 5–12 vs. 15–22 × 6–9 µm), thickened gelatinous sheath with hyaline to olivaceous color ascospores, while N. cerradensis has thin walled and hyaline ascospores (Table 1). Neophyllachora religiosa mainly differs from the extant species in the ascospores characteristics, which has a thick gelatinous sheath that is irregularly thickened, irregularly guttulate at the immature stage and large guttules present at maturity. The shape ranges from globose to elliptical, and a central concave depression present in the globose-shaped ascospores. In this study, all the newly generated sequences clustered together and formed a sister clade to N. fici with high bootstrap support (100%) (Fig. 2). The ITS region comprised 588 aligned characters, including gaps. The base pair comparison between N. fici and the newly obtained sequences shows more than 2% differences in the ITS region without the gap. The base pair comparison among the newly generated sequences revealed a minor difference in the ITS region. Thus, we assumed that the newly generated sequences obtained in this study are from the same species., Published as part of Literatus, Irish C. E., Maharachchikumbura, Sajeewa S. N., Withee, Patchareeya, Haituk, Sukanya, Tamakaew, Nisachon, Nguanhom, Jeerapa, Monkhung, Sararat, Thiyagaraja, Vinodhini & Cheewangkoon, Ratchadawan, 2023, Taxonomic and phylogenetic appraisal of a new holomorphic Neophyllachora species from Chiang Mai, Thailand, pp. 259-271 in Phytotaxa 600 (5) on pages 264-266, DOI: 10.11646/phytotaxa.600.5.1, http://zenodo.org/record/8129400

Key to species of Neophyllachora 1. Parasitic on Ficus, Myrcia and Myrciaria species..............................................................................................................................2 1’. Parasitic on Psidium species, ascospores thin-walled, short-ellipsoidal covered with thin-walled gelatinous sheath............................................................................................................................................................................................................. N. subcircinans 2. Parasitic on Ficus, Myrcia species.....................................................................................................................................................3 2’. Parasitic on Myrciaria species, clavate-fusoid asci........................................................................................................ N. myrciariae 3. Parasitic on Myrcia species, fusoid asci, ascospores covered with gelatinous sheath.......................................................................4 3’. Parasitic on Myrcia species, lunate-reniform to half-moon shape ascospores with thick walled............................. N. truncatispora 4. Elliptic-Oblong ascospores with microguttulate cytoplasm......................................................................................... N. cerradensis 4’. Lunate ascospores with thin-walled................................................................................................................................... N. myrciae 5. Parasitic on Ficus, globose to ellipsoidal ascospores with gelatinous.............................................................................. N. religiosa 5’. Parasitic on Ficus, globose to subglobose ascospores without gelatinous sheath...................................................................... N. fici

Literatus, Irish C. E., Maharachchikumbura, Sajeewa S. N., Withee, Patchareeya, Haituk, Sukanya, Tamakaew, Nisachon, Nguanhom, Jeerapa, Monkhung, Sararat, Thiyagaraja, Vinodhini, Cheewangkoon, Ratchadawan (2023): Taxonomic and phylogenetic appraisal of a new holomorphic Neophyllachora species from Chiang Mai, Thailand. Phytotaxa 600 (5): 259-271, DOI: 10.11646/phytotaxa.600.5.1, URL: http://dx.doi.org/10.11646/phytotaxa.600.5.1

A total of approximately 104,000 fungal species can be expected in Yunnan. However, approximately 6000 fungal species have been reported from the province leaving much to be described. This study introduces a new species, Fitzroyomyces xishuangbannaensis sp. nov and a new collection of Fit. cyperacearum from Yunnan province, China. The taxa were characterized based on morphological characteristics and combined multi-gene phylogenetic analyses. Both maximum likelihood (ML) and Bayesian inference posterior probabilities (PP) were conducted for combined LSU, ITS and mtSSU sequences data. The new Fitzroyomyces species formed a distinct clade among the extant species of Fitzroyomyces with high statistical supports in the phylogenetic analyses while our new isolate nested together with the corresponding strains of Fit. cyperacearum. A key to Fitzroyomyces species and a synopsis table of morphological characteristics for Fitzroyomyces are provided to support the taxonomic placement.

Sclerococcum simplex Hawksworth (1979:249) Index Fungorum no. IF323243; Facesoffungi no. FoF 10100 (Fig. 2) Description of the Chinese specimen: Lichenicolous on Pertusaria sp. Colonies forming discrete patches mainly on the ascomata and rarely on thallus of the host. Asexual morph: Sporodochia 95–185 µm diam., 85–155 µm high (x̄ = 140 × 120 μm, n = 10), stromatic, dark brown, subglobose to irregular, immersed to erumpent, aggregated into dense sporodochia. Vegetative hyphae 1.5–5 μm thick, hyaline to brown, septate, slightly constricted at the septa, irregularly branched, immersed in the host thallus. Conidiophore merismatic, not branched, hyaline to pale brown. Conidiogenous cells 2.5–3 μm thick, monoblastic, terminal, integrated, hyaline or pale brown, not very distinct. Conidia 3–7 × 2–5 µm, (x̄ = 5 × 3.5 μm, n = 20), produced in short basipetal chains, separating easily, acrogenous, ellipsoid or angular, light brown to dark brown, thick wall, mainly non-septate, rarely 2-celled, cells separating easily, the resulting part-conidia often more or less triangular, the lower cell often smaller, all parts becoming slightly darker in K. Sexual morph: unknown. Specimens examined. CHINA, Yunnan Province, Diqing Tibetan Autonomous Prefecture, Shangri-La, (N 27 o 55’ 54.9”, E 099 o 34’ 39.0”, 4045m MSL), on corticolous host thallus of Pertusaria sp., 12 September 2018, V. Thiyagaraja (MFLU 21-0117)., Published as part of Thiyagaraja, Vinodhini, Ertz, Damien, Hyde, Kevin D., Karunarathna, Samantha C., To-Anun, Chaiwat & Cheewangkoon, Ratchadawan, 2022, Morphological and phylogenetic reassessment of Sclerococcum simplex from China, pp. 167-175 in Phytotaxa 559 (2) on page 170, DOI: 10.11646/phytotaxa.559.2.5, http://zenodo.org/record/7021650, {"references":["Hawksworth, D. L. (1979) The lichenicolous Hyphomycetes. Bulletin of the British Museum (Natural History). Botany Series 6: 183 - 300."]}

Fitzroyomyces xishuangbannaensis R.F. Xu & Tibpromma, sp. nov. ( Fig. 3) Index Fungorum Number: IF 559624; Facesoffungi Number: FOF 10811 Holotype: HKAS 122682 Etymology: The specific epithet “xishuangbannaensis” refers to Xishuangbanna, in Yunnan Province where the holotype was collected. Saprobic on dead branch of Hevea brasiliensis. Sexual morph: Apothecial 235–430 high μm, 200–520 diam μm (x̅ = 300 × 400 μm, n = 10), subglobose, unilocular, gregarious, immersed from the substrate, immersed at first and opening by entire pore at maturity. Hypothecium convex. Disc deeply cupulate, whitish to cream. Margins white. Hymenium hyaline, enclosed in a thick gelatinous matrix. Epithecium absent. Excipulum 20–55 μm composed of cells of textura intricata. Paraphyses 1–2 μm wide at the apex, numerous, filiform, septate, unbranched. Asci 160–180× 10–20 μm (x̅ = 173 × 13 μm, n = 11), 8-spored, unitunicate, long cylindrical, short sessile, rounded at the apex. Ascospores 90–180 × 2–4 μm (x̅ = 152 × 3 μm, n = 10), filiform, hyaline, multiseptate, up to 35-septa, finely guttulate when immature, not constricted at the septa and not easily break into different size. Asexual morph: Undetermined. Material examined: CHINA, Yunnan Province, Xishuangbanna, on a dead branch of Hevea brasiliensis Muell. Arg, 30 July 2021, Ruifang Xu, XSBNR–20, (HKAS 122682, holotype). Notes: The nucleotide base pair differences of ITS, LSU and mtSSU sequences are compared between our new species and members of Fitzroyomyces (Table 2). The new taxon was established based on the recommendations outlined by Jeewon & Hyde (2016). We also compared the morphology of our strain with Fit. cyperacearum, Fit. hyaloseptisporus, Fit. pandanicola and Fit. yunnanensis (Table. 3). The differences between Fit. xishuangbannaensis and Fit. cyperacearum are that the larger apothecia (235–430 × 200–520 μm vs. 201–260 × 210–310 μm), wider exciple (17–70 μm vs. 20–55 μm), narrower paraphyses (17–70 μm vs. 24–54 μm), longer asci (110–150 × 10–20 μm vs. 160– 180 × 10–20 μm), longer, and fusiform ascospore guttulate with more septation. Fitzroyomyces xishuangbannaensis differs from Fit. hyaloseptisporus in larger apothecia (235–430 × 200–520 μm vs. 140–200 × 150–200 μm), wider exciple (20–55 μm vs. 6–20 μm), smaller asci (160–180 × 10–20 μm vs. 165–200 × 10–25 μm) and ascospores (90– 180 × 2–4 μm vs. 150–200 × 3.5–6 μm), further, ascospores are fuliform with more septation in Fit. hyaloseptisporus whereas Fit. xishuangbannaensis possesses filiform, hyaline, multiseptate ascospores finely guttulate when immature (Wei et al. 2021). Fit. xishuangbannaensis and Fit. yunnanensis have the same geographical distribution in China (Lu et al. 2021) however, the two differ in several morphological characteristics such as Fit. yunnanensis has bigger apothecia, smaller asci, smaller ascospores and septation (Table 3). A key to species in Fitzroyomyces 1. Septation of ascospores less than 25..................................................................................................................... 2 1. Septation of ascospores more than 25.................................................................................................................. 3 2a. Guttulate fusiform ascospores, textura intricata to textura angularis exciple......................................................... Fit. yunnanensis 2b. Eguttulate filiform ascospores, textura intricate exciple...................................................................................... Fit. cyperacearum 3. Guttulate filiform ascospores............................................................................................................................................................. 4 3. Eguttulate filiform ascospores.................................................................................................................................. Fit. pandanicola 4a. Ascospores commonly presenting 1–3 prominent constrictions (ascospores easily break into different size of fragments........................................................................................................................................................................................... Fit. hyaloseptisporus 4b. Ascospores not constricted at the septa and not easily break into different size......................................... Fit. xishuangbannaensis, Published as part of Xu, Ruifang, Thiyagaraja, Vinodhini, Dai, Dong-Qin, Karunarathna, Samantha C. & Tibpromma, Saowaluck, 2022, Additions to Fitzroyomyces (Stictidaceae, Ascomycota) from Yunnan Province, China, pp. 253-266 in Phytotaxa 548 (2) on page 260, DOI: 10.11646/phytotaxa.548.2.8, http://zenodo.org/record/6597872, {"references":["Jeewon, R. & Hyde, K. D. (2016) Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. Mycosphere 7: 1669 - 1677. https: // doi. org / 10.5943 / mycosphere / 7 / 11 / 4","Wei, D. P., Wanasinghe, D. N., Gentekaki, E., Thiyagaraja, V., Lumyong, S. & Hyde, K. D. (2021) Morphological and phylogenetic appraisal of novel and extant taxa of Stictidaceae from Northern Thailand. Journal of Fungi 7: 880. https: // doi. org / 10.3390 / jof 7100880","Lu, L., Tibpromma, S., Karunaratthan, S. C., Thiyagaraja, V., Xu, J. C., Jayawardena, R. S., Lumyong, S. & Hyde, K. D. (2021) Taxonomic and phylogenic appraisal of one novel species and one new record of Stictidaceae from coffee in Yunnan Province, China. Phytotaxa 528: 111 - 124 https: // doi. org / 10.11646 / phytotaxa. 528.2.4"]}

Ostropomyces Thiyagaraja, L��cking, Ertz & K.D. Hyde, 2021 Ostropomyces was introduced by Thiyagaraja et al. (2021), and the genus has two saprobic members, with O. pruinosellus as the type species. The sexual morph of this genus is characterized by immersed fruiting bodies with long-cylindrical, 4-spored asci and filiform, multi-septate ascospores. The asexual morph is characterized by erumpent pycnidia with ostioles, and filiform conidia which are hyaline, and guttulate at maturity., Published as part of Lu, Li, Tibpromma, Saowaluck, Karunarathna, Samantha C., Thiyagaraja, Vinodhini, Xu, Jianchu, Jayawardena, Ruvishika S., Lumyong, Saisamorn & Hyde, Kevin D., 2021, Taxonomic and phylogenic appraisal of a novel species and a new record of Stictidaceae from coffee in Yunnan Province, China, pp. 111-124 in Phytotaxa 528 (2) on page 118, DOI: 10.11646/phytotaxa.528.2.4, http://zenodo.org/record/5778712, {"references":["Thiyagaraja, V., Lucking, R., Ertz, D., Karunarathna, S. C., Wanasinghe, D. N., Lumyong, S. & Hyde, K. D. (2021) The evolution of life modes in Stictidaceae, with three novel taxa. Journal of Fungi 7 (2): 105. https: // doi. org / 10.3390 / jof 7020105"]}

Fitzroyomyces Crous et al., Persoonia 39: 389 (2017) Fitzroyomyces was introduced by Crous et al. (2017) with Fitzroyomyces cyperi as the type species, which was recorded in its asexual state from leaves of Cyperaceae in Australia. The taxon has similar morphological characteristics to Septoria but clustered within Stictidaceae in phylogenetic analyses (Quaedvlieg et al. 2013, Verkley et al. 2013, Crous et al. 2017). Fitzroyomyces species are saprobic, and the asexual morph characteristics are conidiomata with central ostiole, exuding a creamy conidial mass that comprises hyaline, cylindrical, septate and flexuous conidia (Crous et al. 2017). The sexual morph of F. cyperacearum was recorded from dead stems of Clematis subumbellata and Epilobium angustifolium in Thailand and the U.K. (Ekanayaka et al. 2019, Phukhamsakda et al. 2020). The sexual morph is characterized by cupulate apothecia immersed in the substrate, whitish to cream disc, numerous, long filiform, and aseptate paraphyses, long and hyaline asci, with filiform, multiseptate and hyaline ascospores. Wei et al. (2021) introduced F. hyaloseptisporus and synonymized Stictis pandanicola under Fitzroyomyces based on morphological and phylogenetic analyses. Fitzroyomyces comprises three species, F. cyperacearum, F. pandanicola (= Stictis pandanicola) and F. hyaloseptisporus (Crous et al. 2017, Tibpromma et al. 2018, Wei et al. 2021)., Published as part of Lu, Li, Tibpromma, Saowaluck, Karunarathna, Samantha C., Thiyagaraja, Vinodhini, Xu, Jianchu, Jayawardena, Ruvishika S., Lumyong, Saisamorn & Hyde, Kevin D., 2021, Taxonomic and phylogenic appraisal of a novel species and a new record of Stictidaceae from coffee in Yunnan Province, China, pp. 111-124 in Phytotaxa 528 (2) on page 118, DOI: 10.11646/phytotaxa.528.2.4, http://zenodo.org/record/5778712, {"references":["Crous, P. W., Wingfield, M. J., Burgess, T. I., Carnegie, A. J., Hardy, G. S. J., Smith, D. & Groenewald, J. Z. (2017) Fungal Planet description sheets: 625 - 715. Persoonia 39: 270. https: // doi. org / 10.3767 / persoonia. 2017.39.11","Quaedvlieg, W., Verkley, G. J. M., Shin, H. D., Barreto, R. W., Alfenas, A. C., Swart, W. J. & Crous, P. W. (2013) Sizing up Septoria. Studies in Mycology 75: 307 - 390. https: // doi. org / 10.3114 / sim 0017","Verkley, G. J. M., Quaedvlieg, W., Shin, H. D. & Crous, P. W. (2013) A new approach to species delimitation in Septoria. Studies in Mycology 75: 213 - 305. https: // doi. org / 10.3114 / sim 0018","Ekanayaka, A. H., Hyde, K. D., Jones, E. B. G., Zhao, Q. & Bulgakov, T. S. (2019) New and known discolichens from Asia and eastern Europe. Asian Journal of Mycology 2 (1): 48 - 86. https: // doi. org / 10.5943 / ajom / 2 / 1 / 2","Phukhamsakda, C., McKenzie, E. H. C., Phillips, A. J., Jones, E. G., Bhat, D. J., Stadler, M. & Hyde, K. D. (2020) Microfungi associated with Clematis (Ranunculaceae) with an integrated approach to delimiting species boundaries. Fungal Diversity 102 (1): 1 - 203. https: // doi. org / 10.1007 / s 13225 - 020 - 00448 - 4","Wei, D. P., Wanasinghe, D. N., Gentekaki, E., Thiyagaraja, V., Lumyong, S. & Hyde, K. D. (2021) Morphological and phylogenetic appraisal of novel and extant taxa of Stictidaceae from Northern Thailand. Journal of Fungi 7 (10): 880. https: // doi. org / 10.3390 / jof 7100880","Tibpromma, S., Hyde, K. D., McKenzie, E. H. C., Bhat, D. J., Phillips, A. J., Wanasinghe, D. N. & Karunarathna, S. C. (2018) Fungal diversity notes 840 - 928: micro-fungi associated with Pandanaceae. Fungal Diversity 93 (1): 1 - 160. https: // doi. org / 10.1007 / s 13225 - 018 - 0408 - 6"]}

Ostropomyces pruinosellus Thiyagaraja, L��cking, Ertz & K.D. Hyde, in Thiyagaraja et al., Journal of Fungi 7(2):105 (2021) (Fig. 3) Index Fungorum number: IF 556556; Facesoffungi number: FoF 09512 Saprobic on dead wood of Arabica coffee. Pycnidia with a pruinose appearance on the surface, irregular shape, solitary. Sexual morph: Undetermined. Asexual morph: Pycnidia (excluding neck) 100���300 ��m wide, 80���250 ��m high (x�� = 206 �� 127 ��m, n = 10), immersed, globose to oval, single or clustered under the substrate, with short ostiole. Pycnidial wall 5���35 ��m wide (x�� = 21 ��m, n = 20), transverse section shows two layers; outer layer hyaline, thin-walled, densely packed, and inner layer of textura angularis, hyaline, loosely packed. Conidiophores reduced to conidiogenous cells. Conidiogenous cells 5���10 ��m wide (x�� = 5.5 ��m, n = 10), hyaline, lining the inside wall. Conidia 5���10 �� 2���4 ��m (x�� = 7.5 �� 2.7 ��m, n = 30), allantoid to filiform, aseptate, hyaline, catenate, guttulate at maturity, conidia connect to form a large branch. Culture characteristics:��� Colonies on PDA reaching 15 mm diam after 4 weeks at room temperature (20���25 ���). Culture from above, white, circular, with clusters of bumps that secrete brown liquid, towards the edge with irregular white aerial mycelia. Reverse side changes from dark brown in the middle to lighter on both sides. Material examined:��� CHINA. Yunnan Province: Pu���er, on dead wood of coffee, 23 December 2020, Li Lu, MJ-C5 (HKAS 113187); living culture KUMCC 21-0022. Notes:��� Based on ITS and LSU blast results, our isolate is95%similar to Ostropomyces. pruinosellus (NR _172006), and 98% similar to O. pruinosellus (NG _074507). The new strain formed a group together with O. pruinosellus in the combined mtSSU, LSU and ITS phylogenetic analyses with 100% ML, 1.00 BYPP boostrap support and shares similar morphological characteristics (asexual morph) with O. thailandicus. Our new collection differs from O. thailandicus in its allantoid to filiform conidia, and large branched conidial chains, whereas O. thailandicus has filiform conidia with unbranched chains, but similar in having catenate conidia and pruinose on the surface. Comparison of mtSSU, LSU and ITS regions of our new isolate with O. pruinosellus (MFLU 20-0538) reveals 0/6/13 (-/0.69%/2.4%) base pair differences, with O. pruinosellus ( MFLU 21-0115) 4/0/3 (0.55%/0/0.57%) base pair differences and with O. pruinosellus (MFLUCC 21-0112) 10/4/13 (1.36%/0.47%/2.4%) base pair differences. Therefore, we identify our strain as O. pruinosellus based on guidelines of Jeewon & Hyde (2016). Ostropomyces pruinosellus has been reported as sexual morph from unidentified dicotyledonous plant from Thailand (Thiyagaraja et al. 2021, Wei et al. 2021). Thus, this is first report of the asexual morph of O. pruinosellus with a new host record from coffee and new geographical record from China., Published as part of Lu, Li, Tibpromma, Saowaluck, Karunarathna, Samantha C., Thiyagaraja, Vinodhini, Xu, Jianchu, Jayawardena, Ruvishika S., Lumyong, Saisamorn & Hyde, Kevin D., 2021, Taxonomic and phylogenic appraisal of a novel species and a new record of Stictidaceae from coffee in Yunnan Province, China, pp. 111-124 in Phytotaxa 528 (2) on pages 118-120, DOI: 10.11646/phytotaxa.528.2.4, http://zenodo.org/record/5778712, {"references":["Jeewon, R. & Hyde, K. D. (2016) Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. Mycosphere 7: 1669 - 1677. https: // doi. org / 10.5943 / mycosphere / 7 / 11 / 4","Thiyagaraja, V., Lucking, R., Ertz, D., Karunarathna, S. C., Wanasinghe, D. N., Lumyong, S. & Hyde, K. D. (2021) The evolution of life modes in Stictidaceae, with three novel taxa. Journal of Fungi 7 (2): 105. https: // doi. org / 10.3390 / jof 7020105","Wei, D. P., Wanasinghe, D. N., Gentekaki, E., Thiyagaraja, V., Lumyong, S. & Hyde, K. D. (2021) Morphological and phylogenetic appraisal of novel and extant taxa of Stictidaceae from Northern Thailand. Journal of Fungi 7 (10): 880. https: // doi. org / 10.3390 / jof 7100880"]}

Fitzroyomyces yunnanensis L. Lu, K.D. Hyde & Tibpromma, sp. nov. (Fig. 2) Index Fungorum number: IF 558662; Facesoffungi number: FoF 09896 Holotype:��� CHINA. Yunnan Province: Xishuangbanna, on dead wood of coffee, 24 November 2020, Saowaluck Tibpromma, XSBN-C5 (HKAS 113186, holotype); ex-type living culture KUMCC 21-0002. Saprobic on dead wood of coffee. Sexual morph: Apothecia 550���1000 �� 230���370 ��m (x�� = 800 �� 270 ��m, n = 10), immersed at first, opening by entire pore at maturity, subglobose. Disc deeply cupulate, pale creamy, with whitepruinose margin. Exciple 25���50 ��m wide (x�� = 38 ��m, n = 10), thick-walled, comprising hyaline cells of textura intricata, inner layer composed of hyaline cells of textura angularis. Hamathecium comprising asci and paraphyses. Paraphyses hyaline, 1���2 ��m wide (x�� = 1.8 ��m), numerous, long filiform, unbranched, aseptate. Asci 100���140 �� 5���10 ��m (x�� = 112 �� 8 ��m, n = 10), unitunicate, 6���8-spored, cylindrical to ovoid, broadest at middle, rounded apex and short stalked. Ascospores 80���120 �� 2���3 ��m (x�� = 88 �� 2.7 ��m, n = 10), overlapping uni- to bi-seriate, fusiform, 12���18- septate, hyaline, not easily breaking into different sizes, smooth-walled, guttulate, without a mucilaginous sheath. Asexual morph: Undetermined. Culture characteristics:��� Colonies on PDA reaching 35 mm diam, after 4 weeks at room temperature (20���25 ���). Culture from above, convex with surface, dry, rough, white to pale brown, circular, towards the edge with irregular white aerial mycelia, spreading, slightly covered; reverse brown to yellowish, with radiating white mycelia. Etymology:��� The specific epithet ��� yunnanensis ��� refers to Yunnan Province where the holotype was collected. Notes:��� ITS blast result showed that Fitzroyomyces yunnanensis is 89% similar to F. cyperacearum (MK499349). The results of LSU blast showed that our strain is 96% similar to Stictis sp. (MH260319), while mtSSU results showed it to be 90% similar to Carestiella socia (AY661678). Based on phylogenetic analyses, the new strain clustered with Fitzroyomyces and formed a well-separated clade with 100% ML, 1.00 BYPP boostrap support with the extant species of Fitzroyomyces. Fitzroyomyces yunnanensis differs from extant species of Fitzroyomyces in its larger apothecia, with a clear border between outer and inner layers in the exciple, and shorter asci and ascospores. This is the first Fitzroyomyces species reported from coffee. A synopsis of characters for sexual taxa in Fitzroyomyces is provided (Table 2)., Published as part of Lu, Li, Tibpromma, Saowaluck, Karunarathna, Samantha C., Thiyagaraja, Vinodhini, Xu, Jianchu, Jayawardena, Ruvishika S., Lumyong, Saisamorn & Hyde, Kevin D., 2021, Taxonomic and phylogenic appraisal of a novel species and a new record of Stictidaceae from coffee in Yunnan Province, China, pp. 111-124 in Phytotaxa 528 (2) on page 118, DOI: 10.11646/phytotaxa.528.2.4, http://zenodo.org/record/5778712

Coffee, an important economic crop, is often threatened by fungal infections. During a survey of coffee fungi in Yunnan Province, China, two saprobic Stictidaceae species were collected. Maximum likelihood (ML) and Bayesian posterior probability of combined LSU, ITS and mtSSU genes supported the placement of our fungal collections within Fitzroyomyces and Ostropomyces with high statistical support. A new species, Fitzroyomyces yunnanensis sp. nov. and a new record, Ostropomyces pruinosellus are introduced. These two species were recorded on coffee wood in sexual and asexual states, respectively. Their taxonomic placements were further supported by detailed morphological and phylogenetic comparisons of allied taxa.

Key to species of Alloleptosphaeria 1. Ascomata semi-erumpent to erumpent, ≤ 0.5 mm diam.............................................................................................................................2 1. Ascomata semi-erumpent to superficial, ≥ 0.5 mm diam..................................................................................................... A. shangrilana 2. Asci cylindric-clavate to clavate, overlapping 1–2-seriate.........................................................................................................................3 2. Asci narrowly ellipsoid, multi-seriate........................................................................................................................................ A. iridicola 3. Ascospores globose or subglobose, 3-septate................................................................................................................................ A. italica 3. Ascospores obpyriform, muriform........................................................................................................................................... A. clematidis

Alloleptosphaeria shangrilana, collected from dead wood of a dicotyledonous host in Shangri-La, China, is introduced as a new species based on evidence from morphological data and DNA sequence analyses. Maximum likelihood and Bayesian analyses of combined SSU, LSU and ITS sequence data were used to clarify the phylogenetic affinity of the new species. The new taxon is characterized by semi-immersed to superficial ascomata, a thick peridium, branched and septate pseudoparaphyses, cylindrical asci, muriform ascospores and overlapping uniseriate ascospores. Alloleptosphaeria species have been reported from England, Italy, Thailand and this is the first report of the genus in China. A detailed morphological description and analyses of the phylogenetic results are given for the new species. A key and synopsis table to the genus are also provided.