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Your search keyword '"Tišma, Miloš"' showing total 12 results
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7. Connecting the dots: key insights on ParB for chromosome segregation from single-molecule studies.

Author

Tišma, Miloš, Kaljević, Jovana, Gruber, Stephan, Le, Tung B K, and Dekker, Cees

Subjects
*CHROMOSOME segregation, *CELL division, *NUCLEOIDS, *PLASMIDS, *BACTERIAL cells, *CELL cycle
Abstract

Bacterial cells require DNA segregation machinery to properly distribute a genome to both daughter cells upon division. The most common system involved in chromosome and plasmid segregation in bacteria is the ParABS system. A core protein of this system - partition protein B (ParB) - regulates chromosome organization and chromosome segregation during the bacterial cell cycle. Over the past decades, research has greatly advanced our knowledge of the ParABS system. However, many intricate details of the mechanism of ParB proteins were only recently uncovered using in vitro single-molecule techniques. These approaches allowed the exploration of ParB proteins in precisely controlled environments, free from the complexities of the cellular milieu. This review covers the early developments of this field but emphasizes recent advances in our knowledge of the mechanistic understanding of ParB proteins as revealed by in vitro single-molecule methods. Furthermore, we provide an outlook on future endeavors in investigating ParB, ParB-like proteins, and their interaction partners. [ABSTRACT FROM AUTHOR]

Published
2024
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9. ParB proteins can bypass DNA-bound roadblocks via dimer-dimer recruitment

Author

Tišma, Miloš, Panoukidou, Maria, Antar, Hammam, Soh, Young-Min, Barth, Roman, Pradhan, Biswajit, Barth, Anders, van der Torre, Jaco, Michieletto, Davide, Gruber, Stephan, and Dekker, Cees

Subjects
DNA, Bacterial, Bacterial Proteins, Chromosome Segregation, DNA, Bacillus subtilis/genetics, Bacterial Proteins/genetics, Bacterial Proteins/metabolism, DNA/metabolism, DNA, Bacterial/genetics, DNA, Bacterial/metabolism, Protein Binding, Bacillus subtilis
Abstract

The ParABS system is essential for prokaryotic chromosome segregation. After loading at parS on the genome, ParB (partition protein B) proteins rapidly redistribute to distances of ~15 kilobases from the loading site. It has remained puzzling how this large-distance spreading can occur along DNA loaded with hundreds of proteins. Using in vitro single-molecule fluorescence imaging, we show that ParB from Bacillus subtilis can load onto DNA distantly of parS, as loaded ParB molecules themselves are found to be able to recruit additional ParB proteins from bulk. Notably, this recruitment can occur in cis but also in trans, where, at low tensions within the DNA, newly recruited ParB can bypass roadblocks as it gets loaded to spatially proximal but genomically distant DNA regions. The data are supported by molecular dynamics simulations, which show that cooperative ParB-ParB recruitment can enhance spreading. ParS-independent recruitment explains how ParB can cover substantial genomic distance during chromosome segregation, which is vital for the bacterial cell cycle.

Published
2022

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