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11. Elucidating Electrical Conductive Mechanisms for CaF2–SiO2–CaO–TiO2 Welding Fluxes.

Author

Zhang, Yanyun, Yuan, Hang, Tian, Huiyu, Wang, Zhanjun, and Wang, Cong

Subjects
IONIC conductivity, ELECTRIC conductivity, WELDING, ELECTRIC welding, CHRONOAMPEROMETRY, POLYELECTROLYTES, HIGH temperatures
Abstract

Total electrical conductivity of CaF2–SiO2–CaO–TiO2 welding fluxes has been investigated using the four-electrode method, and the underpinning conductive mechanisms have been clarified. To quantify the contribution of the electronic/ionic conductivity to the total electrical conductivity, electronic transference numbers have been measured using the stepped potential chronoamperometry method. The results show that electronic and ionic conductivity of the fluxes increases with higher temperature, thereby increasing the total electrical conductivity, which indicates that conductivity in the molten fluxes is a thermally activated process. Higher TiO2 content favors electronic conductivity as improved Ti3+/Ti4+ mass ratio facilitates electron hopping. However, electronic conductivity decreases with increasing TiO2 content, which is accompanied by the enhanced degree of depolymerization of the silicate network. In addition, the conductive mechanism of the fluxes is controlled by an electronic-ionic mixed mode. The increased value of electronic conductivity is significantly greater than the reduced value of ionic conductivity with higher TiO2 content, resulting in a net increase for the total electrical conductivity. [ABSTRACT FROM AUTHOR]

Published
2023
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14. Corrigendum

Author

Jia, Yuebin, primary, Kong, Xiangpei, additional, Hu, Kongqin, additional, Cao, Mengqiang, additional, Liu, Jiajia, additional, Ma, Changle, additional, Guo, Siyi, additional, Yuan, Xianzheng, additional, Zhao, Shan, additional, Robert, Hélène S., additional, Li, Cuiling, additional, Tian, Huiyu, additional, and Ding, Zhaojun, additional

Published
2023
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27. Serratia marcescens PLR enhances lateral root formation through supplying PLR-derived auxin and enhancing auxin biosynthesis in Arabidopsis.

Author

Zhang, Chunlei, Yu, Zipeng, Zhang, Mengyue, Li, Xiaoxuan, Wang, Mingjing, Li, Lixin, Li, Xugang, Ding, Zhaojun, and Tian, Huiyu

Subjects
ROOT formation, SERRATIA marcescens, PLANT growth, AUXIN, ARABIDOPSIS, ROOT development, BIOSYNTHESIS
Abstract

Plant growth promoting rhizobacteria (PGPR) refer to bacteria that colonize the rhizosphere and contribute to plant growth or stress tolerance. To further understand the molecular mechanism by which PGPR exhibit symbiosis with plants, we performed a high-throughput single colony screening from the rhizosphere, and uncovered a bacterium (named promoting lateral root, PLR) that significantly promotes Arabidopsis lateral root formation. By 16S rDNA sequencing, PLR was identified as a novel sub-species of Serratia marcescens. RNA-seq analysis of Arabidopsis integrated with phenotypic verification of auxin signalling mutants demonstrated that the promoting effect of PLR on lateral root formation is dependent on auxin signalling. Furthermore, PLR enhanced tryptophan-dependent indole-3-acetic acid (IAA) synthesis by inducing multiple auxin biosynthesis genes in Arabidopsis. Genome-wide sequencing of PLR integrated with the identification of IAA and its precursors in PLR exudates showed that tryptophan treatment significantly enhanced the ability of PLR to produce IAA and its precursors. Interestingly, PLR induced the expression of multiple nutrient (N, P, K, S) transporter genes in Arabidopsis in an auxin-independent manner. This study provides evidence of how PLR enhances plant growth through fine-tuning auxin biosynthesis and signalling in Arabidopsis, implying a potential application of PLR in crop yield improvement through accelerating root development. [ABSTRACT FROM AUTHOR]

Published
2022
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31. A Raman and Multinuclear 29Si, 27Al, and 19F NMR Study on the Structural Roles of CaF2 in SiO2–CaO–Al2O3-based Welding Fluxes.

Author

Tian, Huiyu, Wang, Zhanjun, Zhao, Tan, and Wang, Cong

Subjects
SUBMERGED arc welding, WELDING, MAGIC angle spinning, MELTING points, WELDED joints, ALUMINUM alloys, MAGNETIC resonance, OXYGEN
Abstract

The efficacy and efficiency of submerged arc welding (SAW) are largely dictated by the physicochemical properties of the employed welding fluxes, including melting point, viscosity, and activity, etc., which are inherently rooted in the microstructure of the fluxes. SiO2–CaO–Al2O3-based fluxes are widely employed for welding high strength low alloy (HSLA) steels. In the present study, the structural roles of CaF2 on SiO2–CaO–Al2O3-based welding fluxes have been systematically investigated through Raman and magic angle spinning-nuclear magnetic resonance (MAS-NMR) techniques. The results showed that originally intact flux structures depolymerized upon increasing content of CaF2. Raman and 29Si NMR results demonstrated that Q2 (Qn, n is the number of bridging oxygens (O0) in one [SiO4]-tetrahedron) increased at the cost of Q3(1Al) (Qn(mAl), m means the number of neighboring aluminate groups), which could be well explained by the interruption of the Si-O-Al linkages. Moreover, as confirmed by 27Al and 19F NMR results, the proportion of AlF6 species increased from 9.9 to 34.2 pct, indicating the substitution of Al–F for Al–O bonds and the depolymerization of the flux structure. The unique behavior of F may offer a theoretical basis for fine-tuning structural unit mobility and element transfer behaviors during the actual welding process. [ABSTRACT FROM AUTHOR]

Published
2022
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32. Non‐canonical AUX/IAA protein IAA33 competes with canonical AUX/IAA repressor IAA5 to negatively regulate auxin signaling

Author

Lv, Bingsheng, Yu, Qianqian, Liu, Jiajia, Wen, Xuejing, Yan, Zhenwei, Hu, Kongqin, Li, Hanbing, Kong, Xiangpei, Li, Cuiling, Tian, Huiyu, De Smet, Ive, Zhang, Xian‐Sheng, and Ding, Zhaojun

Subjects
Indoleacetic Acids, Arabidopsis Proteins, fungi, Arabidopsis, food and beverages, Nuclear Proteins, Articles, Plants, Genetically Modified, DNA-Binding Proteins, Plant Growth Regulators, Gene Expression Regulation, Plant, Proteolysis, heterocyclic compounds, Phosphorylation, Signal Transduction
Abstract

The phytohormone auxin controls plant growth and development via TIR1-dependent protein degradation of canonical AUX/IAA proteins, which normally repress the activity of auxin response transcription factors (ARFs). IAA33 is a non-canonical AUX/IAA protein lacking a TIR1-binding domain, and its role in auxin signaling and plant development is not well understood. Here, we show that IAA33 maintains root distal stem cell identity and negatively regulates auxin signaling by interacting with ARF10 and ARF16. IAA33 competes with the canonical AUX/IAA repressor IAA5 for binding to ARF10/16 to protect them from IAA5-mediated inhibition. In contrast to auxin-dependent degradation of canonical AUX/IAA proteins, auxin stabilizes IAA33 protein via MITOGEN-ACTIVATED PROTEIN KINASE 14 (MPK14) and does not affect IAA33 gene expression. Taken together, this study provides insight into the molecular functions of non-canonical AUX/IAA proteins in auxin signaling transduction.

Published
2019

35. Antagonistic Interaction between Auxin and SA Signaling Pathways Regulates Bacterial Infection through Lateral Root in Arabidopsis

Author

Kong, Xiangpei, primary, Zhang, Chunlei, additional, Zheng, Huihui, additional, Sun, Min, additional, Zhang, Feng, additional, Zhang, Mengyue, additional, Cui, Fuhao, additional, Lv, Dongping, additional, Liu, Lijing, additional, Guo, Siyi, additional, Zhang, Youming, additional, Yuan, Xianzheng, additional, Zhao, Shan, additional, Tian, Huiyu, additional, and Ding, Zhaojun, additional

Published
2020
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40. Nanoplastics Promote Microcystin Synthesis and Release from Cyanobacterial Microcystis aeruginosa

Author

Feng, Li-Juan, primary, Sun, Xiao-Dong, additional, Zhu, Fan-Ping, additional, Feng, Yue, additional, Duan, Jian-Lu, additional, Xiao, Fu, additional, Li, Xiang-Yu, additional, Shi, Yi, additional, Wang, Qian, additional, Sun, Jia-Wen, additional, Liu, Xiao-Yu, additional, Liu, Jia-Qi, additional, Zhou, Lin-Lin, additional, Wang, Shu-Guang, additional, Ding, Zhaojun, additional, Tian, Huiyu, additional, Galloway, Tamara Susan, additional, and Yuan, Xian-Zheng, additional

Published
2020
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41. Non‐canonical AUX / IAA protein IAA 33 competes with canonical AUX / IAA repressor IAA 5 to negatively regulate auxin signaling

Author

Lv, Bingsheng, primary, Yu, Qianqian, additional, Liu, Jiajia, additional, Wen, Xuejing, additional, Yan, Zhenwei, additional, Hu, Kongqin, additional, Li, Hanbing, additional, Kong, Xiangpei, additional, Li, Cuiling, additional, Tian, Huiyu, additional, De Smet, Ive, additional, Zhang, Xian‐Sheng, additional, and Ding, Zhaojun, additional

Published
2019
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43. The pre‐mRNA splicing factor RDM16 regulates root stem cell maintenance in Arabidopsis.

Author

Lv, Bingsheng, Hu, Kongqin, Tian, Te, Wei, Kaijing, Zhang, Feng, Jia, Yuebin, Tian, Huiyu, and Ding, Zhaojun

Subjects
RNA splicing, STEM cells, STEM cell niches, ROOT growth, ARABIDOPSIS
Abstract

Pre‐mRNA (messenger RNA) splicing participates in the regulation of numerous biological processes in plants. For example, alternative splicing shapes transcriptomic responses to abiotic and biotic stress, and controls developmental programs. However, no study has revealed a role for splicing in maintaining the root stem cell niche. Here, a screen for defects in root growth in Arabidopsis thaliana identified an ethyl methane sulfonate mutant defective in pre‐mRNA splicing (rdm16‐4). The rdm16‐4 mutant displays a short‐root phenotype resulting from fewer cells in the root apical meristem. The PLETHORA1 (PLT1) and PLT2 transcription factor genes are important for root development and were alternatively spliced in rdm16‐4 mutants, resulting in a disordered root stem cell niche and retarded root growth. The root cap of rdm16‐4 contained reduced levels of cytokinins, which promote differentiation in the developing root. This reduction was associated with the alternative splicing of genes encoding cytokinin signaling factors, such as ARABIDOPSIS HISTIDINE PHOSPHOTRANSFER PROTEIN5 and ARABIDOPSIS RESPONSE REGULATORS (ARR1, ARR2, and ARR11). Furthermore, expression of the full‐length coding sequence of ARR1 or exogenous cytokinin application partially rescued the short‐root phenotype of rdm16‐4. This reveals that the RDM16‐mediated alternative splicing of cytokinin signaling components contributes to root growth. [ABSTRACT FROM AUTHOR]

Published
2021
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