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ObjectiveTo investigate beverage consumption and influence factors of primary and secondary school students， and provide a scientific basis for guiding the reasonable consumption of beverages for middle and elementary school students in Hebei Province.MethodsMulti-stage stratified cluster random sampling method was applied to sample participants， and 3 259 primary and secondary school students were enrolled. Food Frequency Questionnaire was employed to collect beverage consumption situation in the last month.ResultsThe beverage consumption rate of primary and secondary school students in Hebei Province was 66.62% （2 171/3 259）. And the most common beverages were： vegetable protein beverages （28.60%）， carbonated beverages （24.70%）， non-100% fruit and vegetable juices （24.09%）. The consumption rates of plant protein beverages， non-100% fruit and vegetable juice beverages， carbonated beverages， and milk-containing beverages among primary school students were higher than those among middle school students （P

In recent years, the potential value of nonstructural protein (NSP) 2C was well documented for distinguishing foot-and-mouth disease virus (FMDV) in infected animals and vaccinated animals. In order to develop a more sensitive approach to detect natural infected FMDV while there is no interact with vaccinated FMDV, we incorporated a major epitope region of 2C with whole 3AB coding region within NSP and expressed in Escherichia coli. We got a 47.6 kD fusion protein named 2C'3AB. The product showed a specific reactivity with FMDV from serum of infected animal by using Western blotting analysis. This suggests that this protein could be applied to distinguish infected FMDV and vaccinated FMDV. We further compared 2C'3AB protein with 3ABC fusion protein, another available protein used for detecting infected FMDV, using indirect ELISA assay. The results showed that 2C'3AB-ELISA had higher sensitivity than that of 3ABC-ELISA for distinguishing infected FMDV and vaccinated FMDV of sera from epidemic region. Therefore, this recombinant protein 2C'3AB is a good candidate protein to develop more sensitive method to differentiate infected FMDV and vaccinated FMDV from vaccinated animals. This finding will increase our capability to check the infectious virus carrier and finally improve FMDV infection control.

Objective: To purify and to detect reactivity of non-structural proteins 3A, 3B and 2C expressed in the Escherichia coli., Methods: FMDV NSP 3A, 3B and 2C containing the major B-cell antigenic sites were expressed in E. coli. We got renatured 2C protein by lysing of isolated inclusion body using high concentration of urea, and then diluted in a buffer system containing oxidized/reduced glutathione. Purified 3A, 3B and 2C were obtained by Ni-NTA His Bind Resin affinity chromatography. The reactivity of three NSPs with sera of different origin was measured using an indirect ELISA and Western-blot. The reactivity of three proteins was compared with 3ABC and 3D by detecting sera of clinically healthy sheep that were collected from epidemic region of Asia I FMD., Results: Proteins 3A and 3B were solubly expressed in bacteria, and 2C was expressed to form inclusion body. All three products could react specifically with sera from FMDV infected animal by western-blot and ELISA. The high coincident rates were observed between 3A, 3B, 2C and 3ABC., Conclusion: The results would provide useful materials for establishment of immunoelectro-transfer blot (EITB) diagnostic method, which could be used for differentiation of the FMDV infected animals from the vaccinated animals.