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Grasslands in the Qinghai–Tibet Plateau play an important role in preserving ecological security and high biodiversity in this region. However, the distribution of the composition and structure of plant community and the mechanism by which it maintains itself in this region are still poorly understood. Here, we designed 195 grassland plots in 39 grassland sites along an approximately 1700 m elevation gradient on the Northeastern Qinghai–Tibet Plateau. We found that the grassland community height decreased significantly with increasing elevation, whereas community coverage did not significantly change. With increasing elevation, plant species richness (α diversity) increased significantly, but the community variability (β diversity) decreased significantly. The constrained clustering analysis suggested that the α- and β-diversity in the grasslands transformed gradually with elevation, and that three discontinuous points (based on community structure) were observed at elevation of 3640, 4252 and 4333 m. Structural equation modeling (SEM) indicated that the increase in precipitation and the decrease in temperature significantly positively influenced α diversity, which was negatively correlated with β diversity. These results demonstrate a quantitative-to-qualitative change in the community composition and structure along this elevational gradient on the Qinghai–Tibet Plateau.

Geographic altitude is a potent environmental factor for human microbiota and bone mineral density. However, little evidence exists in population-based studies with altitude diversity ranges across more than 3000 m. This study assessed the associations between a wide range of altitudes and bone mineral density, as well as the potential mediating role of microbiota in this relationship.A total of 99,556 participants from the China Multi-Ethnic Cohort (CMEC) study were enrolled. The altitude of each participant was extracted from global Shuttle Radar Topography Mission (SRTM) 4 data. Bone mineral density was measured by calcaneus quantitative ultrasound index (QUI). Stool samples were collected for 16S rRNA gene sequencing (n = 1384). The metabolites of gut microbiota, seven kinds of short-chain fatty acids (SCFAs), were detected by gas chromatography-mass spectrometry (GC-MS, n = 128). After screening, 73,974 participants were selected for the "altitude-QUI" analysis and they were placed into the low-altitude (LA) and high-altitude (HA) groups. Additionally, a subgroup (n = 1384) was further selected for the "altitude-microbiota-QUI" analysis. Multivariate linear regression models and mediation analyses were conducted among participants.A significant negative association between high-altitude and QUI was obtained (mean difference = -0.373 standard deviation [SD], 95% confidence interval [CI]: -0.389, -0.358, n = 73,974). The same negative association was also observed in the population with microbiota data (mean difference = -0.185 SD, 95%CI: -0.360, -0.010, n = 1384), and a significant mediating effect of Catenibacteriumon on the association between altitude and QUI (proportion mediated = 25.2%, P = 0.038) was also noticed. Additionally, the acetic acid, butyric acid, and total amount of seven SCFAs of the low-altitude group were significantly higher than that of the high-altitude group (P 0.05).High-altitude exposure may decrease bone mineral density in adults, thus increasing the risk of osteoporosis. The modulation of gut microbiota may be a potential strategy for alleviating the decrease of bone mineral density.

幽门螺杆菌是人类常见病原体之一，可引起慢性胃炎、消化性溃疡甚至胃癌等疾病。近年来研究发现幽门螺杆菌可由螺旋形变为球形，进入活的非可培养(viable but non-culturable，VBNC)状态，可能对公众健康构成潜在威胁。在该状态下，幽门螺杆菌的形态结构、生理特征发生了改变，能够保持代谢活性但蛋白质表达下降，同时丧失了在培养基上的生长能力。环境因素、抗生素及抑制剂类物质等条件可诱导幽门螺杆菌进入VBNC状态，但VBNC状态幽门螺杆菌能否复苏尚不明确。基于VBNC状态幽门螺杆菌细胞膜完整且具有代谢活性等特性，可使用活菌直接镜检法等经典方法以及反转录聚合酶链反应等分子生物学方法对其进行检测。目前VBNC状态幽门螺杆菌已在水体环境及生物媒介体内检出，且幽门螺杆菌可在人工污染的食品中进入VBNC状态，这给公共卫生和食品安全带来了挑战。因此，研究VBNC状态幽门螺杆菌的变化规律、检测方法等，对今后VBNC状态幽门螺杆菌的防控及其相关机制研究具有重要意义。.

Determining the temperature sensitivities of the decomposition rates of leaf litter and fine root is important for predicting the impact of climate warming on above- and belowground carbon (C) cycles in forest ecosystems. However, the responses of leaf and root decomposition rates to temperature have rarely been examined together. Here, we present the results of paired leaf litter and fine root decomposition experiments at four forest sites spanning 32° latitude in eastern China. The mean annual soil temperature explained the variances of the decomposition rates of the leaf litter (kLeaf, R2 = 0.95) and fine root (kFine root, R2 = 0.86) across the different biomes well and exerted a positive effect on the kLeaf: kFine root ratio. As a result, the sensitivity of the decomposition rate to temperature was significantly higher in the leaf litter (Q10 = 2.17 ± 0.07) than in the fine root (Q10 = 1.40 ± 0.06). The results of structural equation models indicated that the initial C:nitrogen (C:N) ratio exhibited negative effects, and phosphorus (P) cycling related enzymes activity exhibited positive effects on the kFine root when the effects of temperature were controlled. Even when the variables of these biotic factors were added, the soil temperature still exerted a dominant effect on the kLeaf. Our results suggest that temperature directly influences the kLeaf but indirectly affects the kFine root through litter quality and soil decomposers.

Activity level measurement and fusion rules are the two key factors of image fusion. In the fusion method based on neural networks, the activity level measurements are realized by dividing the image into small blocks and predicting the sharpness of each block; then, the global decision graph guiding fusion is generated according to the predicted results. However, these two tasks are serial in nature, which makes it difficult to complete them simultaneously while achieving satisfactory fusion performance. Therefore, a new multi-focus microscopic image fusion method is proposed in this paper to quickly fuse multiple histological microscopic images from different focusing planes to generate full-focus images. The improved SegNet network was used to detect the unfocused regions. Considering that two or more images are needed for fusion, a parallel fusion strategy is proposed herein to generate clear fusion images based on multiple images instead of pairwise decision graphs. Compared with the convolutional neural network, the proposed network has better representation ability and can extract and fuse the most ideal features to provide a more accurate fusion decision. Compared with the traditional Segnet network, it is lightweight, which greatly improves computing speed and achieves real-time fusion.

Knowledge about the impact of altitude and ethnicity on human oral microbiota is currently limited. To obtain the baseline of normal salivary microbiota, we analyzed the bacteria and fungi composition in Tibetan (HY group) and Han population (CD group) living at different altitudes by using next-generation sequencing (NGS) technology combined with PICRUSt and FUNGuild analyses. There were significant differences in oral microbiota composition between the two groups at phylum and genus levels. At the phylum level, the HY group had higher relative abundances of Firmicutes and Ascomycota, whereas the Bacteroidetes and Basidiomycota in the CD group were richer. These changes at the phylum level reflected different dominant genus compositions. Compared with the Han population, Candida, Fusarium, Zopfiella, Streptococcus, Veillonella and Rothia in Tibetan were higher. Surprisingly, the Zopfiella was found almost exclusively in the Tibetan. The PICRUSt and FUNGuild analysis also indicated that the function of the bacterial and fungal communities was altered between the two groups. In conclusion, our results suggest that there are significant differences in oral microbial structure and metabolic characteristics and trophic modes among Tibetan and Han population living at different altitudes. We first established the oral microbiota framework and represented a critical step for determining the diversity of oral microbiota in the Tibetan and Han population.

Protein synthesis on the endoplasmic reticulum (ER) requires the dynamic coordination of numerous cellular components. Together, resident ER membrane proteins, cytoplasmic translation factors, and both integral membrane and cytosolic RNA-binding proteins operate in concert with membrane-associated ribosomes to facilitate ER-localized translation. Little is known, however, regarding the spatial organization of ER-localized translation. This question is of growing significance as it is now known that ER-bound ribosomes contribute to secretory, integral membrane, and cytosolic protein synthesis alike. To explore this question, we utilized quantitative proximity proteomics to identify neighboring protein networks for the candidate ribosome interactors SEC61β (subunit of the protein translocase), RPN1 (oligosaccharyltransferase subunit), SEC62 (translocation integral membrane protein), and LRRC59 (ribosome binding integral membrane protein). Biotin labeling time course studies of the four BioID reporters revealed distinct labeling patterns that intensified but only modestly diversified as a function of labeling time, suggesting that the ER membrane is organized into discrete protein interaction domains. Whereas SEC61β and RPN1 reporters identified translocon-associated networks, SEC62 and LRRC59 reporters revealed divergent protein interactomes. Notably, the SEC62 interactome is enriched in redox-linked proteins and ER luminal chaperones, with the latter likely representing proximity to an ER luminal chaperone reflux pathway. In contrast, the LRRC59 interactome is highly enriched in SRP pathway components, translation factors, and ER-localized RNA-binding proteins, uncovering a functional link between LRRC59 and mRNA translation regulation. Importantly, analysis of the LRRC59 interactome by native immunoprecipitation identified similar protein and functional enrichments. Moreover, [(35)S]-methionine incorporation assays revealed that siRNA silencing of LRRC59 expression reduced steady state translation levels on the ER by ca. 50%, and also impacted steady state translation levels in the cytosol compartment. Collectively, these data reveal a functional domain organization for the ER and identify a key role for LRRC59 in the organization and regulation of local translation.

We investigated the drug resistance of Mycobacterium tuberculosis isolates from patients with tuberculosis (TB) and HIV, and those diagnosed with only TB in Sichuan, China. TB isolates were obtained from January 2018 to December 2020 and subjected to drug susceptibility testing (DST) to 11 anti-TB drugs and to GeneXpert MTB/RIF testing. The overall proportion of drug-resistant TB (DR-TB) isolates was 32.1% (n = 10 946). HIV testing was not universally available for outpatient TB cases, only 29.5% (3227/10 946) cases had HIV testing results. The observed proportion of multidrug-resistant TB (MDR-TB) isolates was almost double than that of the national level, with approximately 1.5% and 0.1% of the isolates being extensively drug resistant and universally drug resistant, respectively. The proportions of resistant isolates were generally higher in 2018 and 2019 than in 2020. Furthermore, the sensitivities of GeneXpert during 2018-2020 demonstrated a downward trend (80.9, 95% confidence intervals (CI) 76.8-85.0; 80.2, 95% CI 76.4-84.1 and 75.4, 95% CI 70.7-80.2, respectively). Approximately 69.0% (7557/10 946) of the TB cases with DST results were subjected to GeneXpert detection. Overall, the DR-TB status and the use of GeneXpert in Sichuan have improved, but DR-TB challenges remain. HIV testing for all TB cases is recommended.

Scope Atherosclerosis (AS) is closely related to gut microbiota. Our previous studies demonstrated that Ligustrum robustum (LR), a flavonoid-rich tea like plant, could mitigate several AS-related risk factors and modulate gut microbiota in animal models and human subjects. But its anti-AS effect and mechanisms remain unclear. Therefore, in this study, we investigated impacts of LR on AS development and explored the potential underlying mechanisms in C57BL/6J and Apoe-/- mice. Methods and results Female C57BL/6J and Apoe-/ - mice were fed a chow diet or high-choline diet, supplemented with vehicle (water) or LR water extract (700 mg/kg) by gavage for 17 weeks. We found that LR attenuated diet-induced AS by reducing serum trimethylamine (TMA) and trimethylamine-N-oxide (TMAO) levels likely by modulating gut microbiota. Moreover, LR increased the abundance of the genus Bifidobacterium, which generated bile salt hydrolase, and thus presumably enhanced bile acid (BA) deconjugation and increased fecal BA excretion. Meanwhile, LR increased fecal cholesterol excretion, decreased the levels of serum and hepatic cholesterol, but did not affect short-chain fatty acids (SCFAs) in feces. Conclusion LR attenuated AS development presumably by decreasing serum TMAO levels and increasing fecal BA excretion likely via gut microbial modulation. These effects were accompanied by increases in fecal cholesterol excretion, decreases in serum and hepatic cholesterol. This article is protected by copyright. All rights reserved.

The endoplasmic reticulum (ER) is a nexus for mRNA localization and translation, and recent studies have demonstrated that ER-bound ribosomes also play a transcriptome-wide role in regulating proteome composition. The Sec61 translocon (SEC61) serves as the receptor for ribosomes that translate secretory/integral membrane protein-encoding mRNAs, but whether SEC61 also serves as a translation site for cytosolic protein-encoding mRNAs remains unknown. Here, using a BioID proximity-labeling approach in HEK293T Flp-In cell lines, we examined interactions between ER-resident proteins and ribosomes in vivo. Using in vitro analyses, we further focused on bona fide ribosome interactors (i.e. SEC61) and ER proteins (ribophorin I, leucine-rich repeat–containing 59 (LRRC59), and SEC62) previously implicated in associating with ribosomes. We observed labeling of ER-bound ribosomes with the SEC61β and LRRC59 BioID reporters, comparatively modest labeling with the ribophorin I reporter, and no labeling with the SEC62 reporter. A biotin pulse-chase/subcellular fractionation approach to examine ribosome exchange at the SEC61β and LRRC59 sites revealed that, at steady state, ribosomes at these sites comprise both rapid- and slow-exchanging pools. Global translational initiation arrest elicited by the inhibitor harringtonine accelerated SEC61β reporter-labeled ribosome exchange. RNA-Seq analyses of the mRNAs associated with SEC61β- and LRRC59-labeled ribosomes revealed both site-enriched and shared mRNAs and further established that the ER has a transcriptome-wide role in regulating proteome composition. These results provide evidence that ribosomes interact with the ER membrane via multiple modes and suggest regulatory mechanisms that control global proteome composition via ER membrane-bound ribosomes.

The present study provides an overview of the coronavirus disease 2019 (COVID-19) outbreak which has rapidly extended globally within a short period. COVID-19 is a highly infectious respiratory disease caused by a new coronavirus known as SARS-CoV-2 (severe acute respiratory syndrome-coronavirus-2). SARS-CoV-2 is different from usual coronaviruses responsible for mild sickness such as common cold among human beings. It is crucial to understand the impact and outcome of this pandemic. We therefore overview the changes in the curves of COVID-19 confirmed cases and fatality rate in China and outside of China from 31st of December 2019 to 25th of March 2020. We also aimed to assess the temporal developments and death rate of COVID-19 in China and worldwide. More than 414,179 confirmed cases of COVID-19 have been reported in 197 countries, including 81,848 cases in China and 332,331 outside of China. Furthermore, 18,440 infected patients died from COVID-19 infection; 3,287 cases were from China and 15,153 fatalities were reported worldwide. Among the worldwide infected cases, 113,802 patients have been recovered and discharged from different hospitals. Effective prevention and control measures should be taken to control the disease. The presented Chinese model (protocol) of disease prevention and control could be utilized in order to curb the pandemic situation.

Extensive grazing activity is threatening the alpine grassland on the Qinghai-Tibetan Plateau. Evidence showed that grazing exclusion may change the composition, structure and functions of grassland ecosystems. However, such effects depends on the intensity and duration of exclusion. We explored the effects of short-term (2 and 4 years) and long-term (9 and 11 years) grazing exclusion on plant height, coverage and diversity and community heterogeneity in the alpine grassland on the Qinghai-Tibetan Plateau. We found no difference in plant diversity between the short-term grazing exclusion and control. However, long-term grazing exclusion reduced species richness and increased the Simpson dominance index. This decrease in plant species richness mainly contributed to the decrease in common species richness (defined as species with a relative coverage of 1~5%). In addition, community heterogeneity (coefficient of variation, CV) was significantly higher in the long-term grazing exclusion than in the controlled plots. A structural equation modeling (SEM) demonstrated that long-term grazing exclusion increased the community heterogeneity mainly by reducing species diversity. These results suggest that the effects of grazing exclusion on the composition, structure and community spatial heterogeneity of the alpine grassland ecosystem are dependent on the exclusion duration. Grazing activity may maintain high biodiversity and community stability of the alpine grassland in the harsh environment of the Qinghai-Tibetan Plateau.

Ligustrum robustum (LR) shows antiobesity effects in animal studies. However, little is known about the effect on human. The present study aimed to investigate the effect of LR intake on weight change in obese women and the role of gut microbiota. Thirty overweight and obese female participants (BMI ≥24 kg/m2) were recruited in the current study. The participants drank LR 10g/d for 12 wks. Their body composition and related biomarkers were assessed. Alterations of the gut microbiota were analyzed using 16S rRNA sequencing. The primary outcome was the change in body weight. LR intake resulted in 2.5% weight loss over 12 wks (P Bacteroidaceae, Bacteroides, Bacilli, and Lactobacillales were higher while Ruminococcaceae, Enterobacteriaceae, Enterobacteriales, Lachnospiraceae, Clostridia, and Clostridiales were lower at 12 wk. Moreover, LR intervention decreased fasting glucose (Pβ (P LR intervention moderately decreased the body weight in overweight and obese women and such effect might be due to modulation of gut microbiota.

The side ditch is a longitudinal ditch set up on both sides of the road cutting to collect and remove precipitation from the road surface, road shoulder and side slope. It is the most common drainage facility in highway construction. However, the existing side ditches are generally wide, large, and deep, with a single structure, and poor coordination with the environment along the line. For this reason, this paper proposes a new type of permeable ecological side ditch structure design. Under the premise of ensuring the basic functions of the side ditch, this design effectively solves the problem that the edge of the side ditch is susceptible to erosion due to accumulated rainwater or frost heave in the alpine areas with high groundwater level. At the same time, to maximize the strength of the side ditch and enhance its ecological function.

Behavioral response experiment of fish to mirror images is one of the main ways to test the social and aggressive behavior. The behavior changes of Zrbrafish Danio rerio in tanks contain different number of mirrors was tested in this study. The result indicated that the latency of zebrafish from entry tank which contains mirrors to start moving (P < 0.05) and their frequency of midline crossing (P < 0.001) were all lower than control group (contains no mirror), and decreased with increasing numbers of mirrors. The total swimming time of zebrafish in tank contains mirrors increased with increasing numbers of mirrors and significantly higher than control group (P < 0.001). Additionally, the time spent of zebrafish in side that contains mirrors significantly higher than the reverse side (P < 0.05), and this difference degree increased with increasing numbers of mirrors.

For better understanding the phosphorus (P) cycle, it is essential to distinguish the contents of different P speciation in sediments. In the study, the content of total phosphorus (TP) in the sediments ranged from 88.80 to 227.56 μg·g-1, with an average of 138.42 μg·g-1. The content of inorganic phosphorus (IP) ranged from 84.21 to 224.26 μg·g-1, accounting for 88.74%-98.55% of the TP content, which was the main occurrence form in the intertidal sediments of the Yellow River Delta. the contents of phosphorus fractions in surface sediments were 37.06 to 106.35 μg·g-1 for detrital phosphorus (De-P), 2.99 to 34.39 μg·g-1 for exchangeable phosphorus (Ex-P), 5.27-106.06 μg·g-1 for iron bound phosphorus (Fe-P). On the whole, the existing forms of inorganic phosphorus could be ranked as follows: De-P > Fe-P > Ex-P > authigenic phosphorus (Au-P). The regression analysis between TOC content and various forms of phosphorus showed that TOC content was negatively correlated with De-P.

Due to the influence of human factors and natural factors, the oviposition grounds and living environment of fish have been seriously damaged, and the decline of fishery resources is obvious. In recent years, artificial fish nests are widely served as a good means to restore fisheries resources and their degraded habitats, summarizes the materials, structure, nesting methods and existing problems for artificial fish nest, according to the artificial fish nest which is complicated to make, has a short service life and the existing artificial fish nest only aiming at the shortcomings of the herbaceous oocytes, we designed a compound artificial fish nest. The complex artificial fish nest consists of a frame, which is provided with two kinds of soft and hard egg adhesive medium to provide ecologic spawning conditions for grass spawning, gravel spawning and water spawning fish whose spawning ground is damaged, so as to increase and improve the population structure and quantity of wild fish.

Tourists are the main users of the park, whose spatial and temporal distribution and behavioral characteristics are the most real reflection of the construction of the park environment. Tourists in Beihai Park were selected as the research object. Through field investigation and interview investigation, the main behavior characteristics of tourists in different time and space in winter were studied. Finally, from the perspectives of tourists relation with time and space, we analyze the existing problems, understand the causes of these phenomena, and then put forward the corresponding countermeasures and suggestions, and make suggestions on the construction and management of such parks.

The Yangtze River deepwater channel in the downstream of Nanjing China is the largest water transport project. It necessitates an effective ecological risk assessment of sediment–bound heavy metals driven by construction of the Yangtze River ship channel. We conducted a three–year field studies to investigate levels of heavy metals (Hg, Cd, Pb, Cu, and Zn), pH, and total phosphorus in sediments, as well as diversity of zoobenthos, phytoplankton, and zooplankton along the Taichang–Nantong reaches of the Yangtze River. Results showed that high–concentrations of Pb is at 130.62 mg/kg and of Hg is at 0.88 mg/kg in the sediments, which mainly emitted from the power electronics, power systems, and the hydrocarbon–based fuel combustion via principal component analysis. Based on optimized contamination indices for metals, we should consider their ecological risks when the potential ecological risk factor ( E r i ) of Hg is up to 210 in the basin. The channel construction resulted in significantly increasing concentrations and ecological risks of heavy metals at the lower reaches. Meanwhile, the community structure of aquatic organisms changed and the investigated species richness decreased significantly from 78 to 47 during the project. Through redundancy analysis, more significant variances of the plankton species richness (55% and 22%) were attributed to E r i of heavy metals and their combined risk index as compared with both planktonic and benthic organisms (52% and 17%). Moreover, the species density of both Melosira granulata and Navicula sp., major phytoplankton species in the basin, were significantly negatively correlated to Pb and Hg in sediments. Plankton was more sensitive indicators than zoobenthos due to the ability of plankton to reactivate and bioaccumulate sediment–bound heavy metals. This study innovatively warned management to control river engineering–induced ecological risks of metals in sediments and revealed their potential risk pathway of ingestion by sensitive plankton.

Objectives Human cytomegalovirus (HCMV) is an important pathogen causing morbidity and mortality in children. HCMV prevalence in children with respiratory infections has not been investigated in West China. Previous studies have suggested that glycoproteins genotypes may be associated with different clinical presentations, but the associations were controversial. The aim of this study was to determine the prevalence of HCMV infection in children with respiratory infections, the distributions of gB, gO genotypes among these isolates and their potential predictive roles for the development of symptoms in children. Methods 1709 respiratory specimens were obtained from hospitalized children with respiratory symptoms from 2009 to 2014 for the confirmation of HCMV infection. Glycoprotein B,O genotyping was carried out by multiplex nested PCR and sequencing. Results The overall infection rate was 10.8%, and dominant genotypes were gB1 (74.2%) and gO1 (37.1%). Clinical characteristics differed between infants and children >1 year of age. Infants infected with HCMV had a higher frequency of fever (P < 0.001), cough (P < 0.001), rhinorrhea (P < 0.001), expectoration (P = 0.001) and diarrhea (P = 0.005). Children

Human respiratory syncytial virus (HRSV) is a major pathogen that causes worldwide seasonal epidemic disease in infants due to its genetic variations. However, published information on the molecular epidemiology of HRSV was never reported particularly in Chengdu of West China. During five consecutive seasons (from 2009 to 2014), 433 (23.7%) of 1827 samples from hospitalized patients were identified as HRSV positive. Epidemiological characteristics of HRSV revealed that subtype A viruses (62.7%) prevailed in the first three epidemic seasons and faded in the next two seasons, while subtype B viruses (37.3%) kept circulating in five epidemic periods. According to the phylogenetic analysis of glycoprotein (G) gene, five HRSV genotypes NA1, ON1, BA9, BA-C, and CB1 were found in Chengdu. The predominant circulating genotype changed from NA1 in the period of 2010-2012 to BA9 of 2013-2014. The newly emerging ON1 was first reported in West China in October 2013. The early genotypes BA-C and CB1 were replaced by the prevailing BA9 after the third epidemic peak. Genetic mutations in glycosylation sites of G protein were found in HRSV variants, suggesting the virus is able to escape the immune recognition and attack. This study elucidated the local HRSV epidemic was associated with the alternate circulation of multiple genotypes and with the change of glycosylation sites of G protein. J. Med. Virol. 89:32-40, 2017. © 2016 Wiley Periodicals, Inc.

2015年11月30日，第21届联合国气候变化大会在巴黎举行。中国作为目前年碳排放量最大的国家与全球应对气候变化事业的积极参与者备受瞩目。巴黎大会前，中国在“十二五”期间的大力减排、在哥本哈根气候大会上做出的郑重承诺、在会前与美国等主要经济体分别发表了应对气候变化的多个联合声明以及向联合国提交的“国家自主贡献文件”（INDC）均表明中国在以最大的决心、最大的执行力走绿色发展之路。而会上，中国态度、中国方案与中国行动，特别是“中国的碳排放是否能在2030年达到峰值”更是各方密切关注的问题。北京大学城市与环境学院方精云院士领导的研究团队对此给出了一个肯定的回答:“中国有很大的可能在2030年前达到碳排放峰值。” 这项研究结果以“When will China achieve its carbon emission peak?”为题，发表在《国家科学评论》（National Science Review, NSR）上，并在巴黎峰会上进行了展示，引起了强烈反响。法国国际新闻频道FRANCE 24 对此进行了专题采访。 在研究中，作者基于Kaya恒等式, 将碳排放分解为GDP与碳强度（单位GDP的碳排放量）的乘积, 并设置了六种情景进行分析。六种情景分别代表：中国延续以往（1980~2010年）的减排力度；中国未来的碳强度按照与发达国家相似的衰减过程变化；中国继续维持十二五规划的减排力度；中国实现哥本哈根承诺，使得碳强度在2020年时相比2005年下降40%，并以此速度继续减排；中国实现《国家自主贡献》的目标，碳强度在2030年时相比2005年下降60%并延续此减排速度；中国以最低减排速度恰巧在2030年时达到碳排放峰值。 通过对六种情景的比较与分析，结果显示：中国的碳排放量到达峰值的时间为2023~2030年, 对应的峰值为2.89~3.29 Pg C。若中国的碳强度能在未来维持每年下降3.3%，或者中国能达成哥本哈根承诺与INDC目标，并维持十二五规划的减排力度，那么中国就可以实现碳排放在2030年到达峰值的目标。 文中还指出，中国制定的减排目标，远比“八国集团”历史上的排放目标要严苛的多，这说明中国为推动全球携手共进，凝聚各方共识，做出了自己的努力，承担着更多的减排责任。同时，随着一系列减排目标、政策的出台与碳交易市场的开放, 中国有望通过市场机制来优化碳排放配置, 同时督促企业自觉节能减排、改进工艺, 激发企业、公众参与环境管理; 碳捕集与封存(CarbonCapture and Storage, CCS)等新技术的开发与推广使得碳强度的加速下降成为可能; 中国有机会通过巴黎谈判等会议获得来自发达国家的技术与资金支持。以上因素都可能加速中国的减排过程，使得中国的碳排放到达峰值的时间早于2030年。但值得注意的是，碳排放越早到达峰值，意味着商业、企业和消费者用于调整的时间就越少, 更多的基础设施将被过早更换, 对产业结构、能源结构及技术改进等因素的要求越高,相应的减排压力越大。因此，最理想的情况是达成各减排目标的同时, 年碳排放量峰值年份尽量趋近2030年。这需要政府在实际操作中结合经济的宏观调控, 鼓励清洁能源的发展, 发展新技术, 促进碳交易, 从而实现经济可持续发展与环境保护的平衡。

In view of the lack of mandatory statistics on energy consumption of transportation infrastructure at the national level, the energy consumption index system of Qinghai highway transportation infrastructure was established based on the analytic hierarchy process by using fuzzy synthetic evaluation method. And the calculation method of Qinghai highway transportation infrastructure was established based on the calculation results of the unit energy consumption coefficient of each classification index. This work offers reference for the rapid evaluation of the energy consumption of Qinghai highway transportation infrastructure.

Evaluation on the emission reduction effect of the carbon trading pilot is very important for China’s energy conservation and emission reduction. This paper uses the generalized linear model to evaluate the emission reduction effects of China’s carbon trading pilot. The results show that existing Carbon Trading Pilot slow down the relative increase of carbon emissions in the pilot areas, and their emission reduction effect is relatively obvious before and after the establishment of the carbon trading pilots from 2010 to 2012. However, as time goes by, the emission reduction effects in the adjacent period become weak. Therefore, it is necessary to implement the national carbon market as soon as possible to promote the energy saving and emission reduction of national province scale.

Value capture has been shown to be an innovative instrument to solve the dilemma of funding shortfalls for protected areas. However, value capture mechanisms are often ineffective even though financing from the domestic budget is sufficient. This paper structures a theoretical framework to compare two value capture mechanisms (ecological compensation and ecological migration) based on implementation effects (heritage protection and welfare promotion) and transaction costs (the costs of information, bargaining, and policing). Sanjiangyuan National Park in China is adopted as an empirical case. Based on multi-source data collection and in-depth interviews, we review the ecological compensation in multiple functions and ecological migration in multiple forms for this case. Our results suggest that value capture tools have attained great achievements and dramatically changed the lives of aborigines. We then evaluate implementation effects separately: compensation for ecological regulation, bonuses for incentives, and transfer payments to the poor could all contribute to better ecological restoration and increased resident income. However, although ecological engineering accounts for the largest expenditures, it has little influence on heritage protection and welfare promotion. We also find that the transaction costs of ecological migration are significantly higher than those of ecological compensation. To our knowledge, this investigation is the first attempt to understand domestic funds for protected areas from a novel value capture perspective, and the results of this study can provide guidance for both protected area finances and policy evaluation.

Background Acute respiratory infection (ARI) is the leading cause of morbidity and mortality in pediatric patients worldwide and imposes an intense pressure on health care facilities. Data on the epidemiology profiles of ARIs are scarce in the western and rural areas of China. The purpose of the current study is to provide data on the presence of potential pathogens of ARIs in hospitalized children in Chengdu, west China. Methods Respiratory specimens were obtained from hospitalized patients (under 6 years old) with ARIs in a local hospital in Chengdu. Eight respiratory viruses were identified by PCR and 6 respiratory bacteria by biochemical reactions and Analytical Profile Index (API). Pathogens profiles, clinical characteristics and seasonality were analyzed. Results Fifty-one percent of patients were identified with at least one respiratory pathogen. Human rhinovirus (HRV) (23%), Respiratory syncytial virus (RSV) (22.7%) was the most commonly identified viruses, with Klebsiella pneumoniae (11.5%) the most commonly identified bacterium in the study. The presences of more than one pathogen were found, and multiple viral, bacterial, viral/bacterial combinations were identified in 14.9, 3.3 and 13.9% of patients respectively. Respiratory viruses were identified throughout the year with a seasonal peak in December–February. Pathogens profiles and clinical associations were different between infants ( 1 year of age). Infants with ARIs were more likely to have one or more viruses than older children. Infants identified with multiple pathogens had significantly higher proportions of tachypnea than infants that were not. Conclusions This study demonstrated that viral agents were frequently found in hospitalized children with ARI in Chengdu during the study period. This study gives us better information on the pathogen profiles, clinical associations, co-infection combinations and seasonal features of ARIs in hospitalized children, which is important for diagnoses and treatment of ARIs, as well as implementation of vaccines in this area. Moreover, future efforts in reducing the impact of ARIs will depend on programs in which available vaccines, especially vaccines on RSV, HRV and S. pneumoniae could be employed in this region and new vaccines could be developed against common pathogens.

This paper presents a design of a corneal cross-linking instrument with the function of tracking and locating the central position of the pupil in real time. According to the application requirements, based on the principle of uniform light of compound eye lens, the optical projection system of the corneal crosslinking instrument with the diameter of the treatment spot being 9mm and the uniformity being greater than 90% was designed. And put forward a kind of the pupil localization algorithm, the acquisition of the pupil image gray processing and calculate the histogram, according to the characters of histogram adaptively to take out the appropriate threshold binarization, again after the binarization of image contour tracking, get accurate boundary, the outline of the final vote based on the sliding arc length increment method to determine the pupil center and radius. Experimental results show that the corneal cross-linking system designed in this paper can not only accurately project the treatment of light spot, but also accurately track the central position of the pupil of the human eye.

There are four components of carbon (C) pools in a natural forest ecosystem: vegetation, soil, litter and woody debris. Quantifying these C pools and their contributions to forest ecosystems is important in understanding C cycling in forests. Here, we investigated these four C pools in nine beech (Fagus L., Fagaceae) forests along an altitudinal gradient in southwest China. We found that the C pools of beech forest ecosystems ranged from 190.7 to 503.9 Mg C ha−1, mainly attributed to vegetation C (accounting for 33.7–73.9 %) and soil C (accounting for 24.6–65.4 %). No more than 4 % of ecosystem C pools were stored in woody debris (0.25–3.4 %) and litter (0.2–0.7 %). Ecosystem C storage increased significantly with altitude, where the vegetation and woody debris C pools increased concomitantly with increasing altitude, while those of litter and soil exhibited no significant variations. The forest stand age was found to be a key driver of such altitudinal patterns, especially for vegetation C storage. The present study provides reliable data for understanding the structure and function of Chinese beech forests, and emphasizes the importance of considering the influence of stand age on C accumulation.

Objectives Rotaviruses are the most common cause of severe diarrhoeal disease in young children. However, little is known about the epidemiological and clinical profile of rotavirus A (RVA) in diarrhoeal children or the efficacy of Lanzhou lamb rotavirus vaccine (LLR) in Chengdu, China. This study aimed to determine the prevalence and clinical profile of RVA in diarrhoeal children and provide gene analysis information for RVA vaccination programmes. Methods A total of 1121 faecal samples were collected from outpatient children with diarrhoea between 2009 and 2014. RT-PCR was performed to detect RVA infection and other gastroenteritis viruses. VP4 and VP7 genes of 13 RVA strains were sequenced to compare their similarity with vaccine strains. Results The overall RVA infection rate was 17.48%. G1 (54.72%) and G3 (18.87%) were the predominant G genotypes; P[8] (72.36%) and P[4] (11.38%) were the main P genotypes. Sixteen genotypes were identified; G1P[8] (57.33%) and G9P[8] (12.00%) were the most prevalent. The proportion of coinfection with RVA and other gastroenteritis viruses was 18.88%. RVA was mostly detected in winter and in diarrhoeal children 1-2 years of age. The genotypes of Rotarix and RotaTeq vaccines were consistent with RVA strains prevalent in Sichuan and shared high identity. Conclusions RVA was one of the major aetiological agents of diarrhoeal children in Chengdu. Genotype distribution differed within each year and the gene analysis implied low efficacy of LLR. Continuous epidemiological monitoring of RVA is essential for the national vaccination programme.

A primary question in dengue virus (DENV) biology is the molecular strategy for recruitment of host cell protein synthesis machinery. Here, we combined cell fractionation, ribosome profiling, and transcriptome sequencing (RNA-seq) to investigate the subcellular organization of viral genome translation and replication as well as host cell translation and its response to DENV infection. We report that throughout the viral life cycle, DENV plus- and minus-strand RNAs were highly partitioned to the endoplasmic reticulum (ER), identifying the ER as the primary site of DENV translation. DENV infection was accompanied by an ER compartment-specific remodeling of translation, where ER translation capacity was subverted from host transcripts to DENV plus-strand RNA, particularly at late stages of infection. Remarkably, translation levels and patterns in the cytosol compartment were only modestly affected throughout the experimental time course of infection. Comparisons of ribosome footprinting densities of the DENV plus-strand RNA and host mRNAs indicated that DENV plus-strand RNA was only sparsely loaded with ribosomes. Combined, these observations suggest a mechanism where ER-localized translation and translational control mechanisms, likely cis encoded, are used to repurpose the ER for DENV virion production. Consistent with this view, we found ER-linked cellular stress response pathways commonly associated with viral infection, namely, the interferon response and unfolded protein response, to be only modestly activated during DENV infection. These data support a model where DENV reprograms the ER protein synthesis and processing environment to promote viral survival and replication while minimizing the activation of antiviral and proteostatic stress response pathways. IMPORTANCE DENV, a prominent human health threat with no broadly effective or specific treatment, depends on host cell translation machinery for viral replication, immune evasion, and virion biogenesis. The molecular mechanism by which DENV commandeers the host cell protein synthesis machinery and the subcellular organization of DENV replication and viral protein synthesis is poorly understood. Here, we report that DENV has an almost exclusively ER-localized life cycle, with viral replication and translation largely restricted to the ER. Surprisingly, DENV infection largely affects only ER-associated translation, with relatively modest effects on host cell translation in the cytosol. DENV RNA translation is very inefficient, likely representing a strategy to minimize disruption of ER proteostasis. Overall these findings demonstrate that DENV has evolved an ER-compartmentalized life cycle; thus, targeting the molecular signatures and regulation of the DENV-ER interaction landscape may reveal strategies for therapeutic intervention.

BackgroundThe prevalence of drug-resistant tuberculosis (DR-TB) has brought severe challenges to the prevention and control of tuberculosis. Studies have explored the status of antituberculosis drug (ATD) resistance in different regions of China. However, few studies have focused on DR-TB in Sichuan to date. Due to the large population in Sichuan, detailed investigations of the DR-TB burden in Sichuan are needed. The objective of this study was to investigate the drug resistance characteristics of TB isolates from tuberculosis patients with and without HIV (TB-HIV patients and TBw/oHIV patients) in Chengdu, Sichuan, China.MethodsIsolates from respiratory samples of TBw/oHIV patients and TB-HIV patients hospitalized between January 2014 and December 2017 were collected. Nontuberculosis mycobacteria (NTM) were excluded. Drug sensitivity testing (DST) was performed according to the dilution method in microplates with 4 first-line ATDs and 8 second-line ATDs. TB strains were separated according to patient treatment history, patient age, calendar year and GeneXpert MTB/RIF (GeneXpert) assay results for further analysis.ResultsFor the 7470 patients recruited, the multidrug-resistant tuberculosis (MDR-TB) rate was 2.1-fold (14.6% vs. 6.8%) higher than the national baseline level. The repeatedly admitted patients were more likely to have a resistance profile than the first-time-admitted cases in both the TB-only group (P2015>2016≈2017 for TBw/oHIV patients. The same trend was also observed for TB-HIV patients: 2014>2015>2016≈2017. The GeneXpert TB-positive rate in the TBw/oHIV group was higher than that in the TB-HIV group [81%(639/792) vs. 65% (13/20), PConclusionIn Sichuan, ATD resistance has improved since 2014, but to date, it remains severe. The different resistance profiles of TBw/oHIV patients and TB-HIV patients indicates the need for personalized treatment plans. Specifically, the GeneXpert assay might be more suitable for TBw/oHIV patients than for TB-HIV patients.

Viral acute gastroenteritis (AGE) is a major cause of mortality in childhood and leads to hospitalization in developing countries. Human bocavirus (HBoV) is a newly described virus associated with gastroenteritis. However, little is known about the clinical and epidemiological profile of human bocavirus (HBoV) in pediatric population in Chengdu, China. Between January 2012 and December 2013, 346 fecal samples from children admitted to hospital with acute gastroenteritis were tested for the presence of HBoV as well as other gastroenteritis viruses. Additionally, laboratory test results, clinical features and epidemiological characters were also collected to assess the correlation between HBoV and acute gastroenteritis in pediatric patients in Chengdu, China. Of the 346 tested fecal samples, 60 (17.34%) were positive for HBoV. The rate of co-detection of HBoV with other gastroenteritis viruses was 43.33% (26/60). HBoV was most detected in the summer/early fall months. Sequence analysis of the VP1/VP2 gene showed that both HBoV1 and HBoV2 circulated in Chengdu, China. No obvious difference was found between the clinical features of the HBoV1 mono-detected patients and HBoV2 mono-detected patients. Besides, in diarrheic children, positive correlation was found between detection of HBoV and diagnose of respiratory disease. Furthermore, blood cells were detected more frequently in fecal samples of patients with HBoV mono-detected. Though HBoV was detected frequently in Children with diarrhea in Chengdu, no statistical association was found between HBoV and disease severity.

Human respiratory syncytial virus (HRSV) is a major pathogen that causes worldwide seasonal epidemic disease in infants due to its genetic variations. However, published information on the molecular epidemiology of HRSV was never reported particularly in Chengdu of West China. During five consecutive seasons (from 2009 to 2014), 433 (23.7%) of 1827 samples from hospitalized patients were identified as HRSV positive. Epidemiological characteristics of HRSV revealed that subtype A viruses (62.7%) prevailed in the first three epidemic seasons and faded in the next two seasons, while subtype B viruses (37.3%) kept circulating in five epidemic periods. According to the phylogenetic analysis of glycoprotein (G) gene, five HRSV genotypes NA1, ON1, BA9, BA-C, and CB1 were found in Chengdu. The predominant circulating genotype changed from NA1 in the period of 2010-2012 to BA9 of 2013-2014. The newly emerging ON1 was first reported in West China in October 2013. The early genotypes BA-C and CB1 were replaced by the prevailing BA9 after the third epidemic peak. Genetic mutations in glycosylation sites of G protein were found in HRSV variants, suggesting the virus is able to escape the immune recognition and attack. This study elucidated the local HRSV epidemic was associated with the alternate circulation of multiple genotypes and with the change of glycosylation sites of G protein. J. Med. Virol. 89:32-40, 2017. © 2016 Wiley Periodicals, Inc.