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6 results on '"Timmermans, Rian A. H."'

2. Effects of High-Pressure Processing, UV-C Irradiation and Thermoultrasonication on Donor Human Milk Safety and Quality

Author

Kontopodi, Eva, Stahl, Bernd, van Goudoever, Johannes B, Boeren, Sjef, Timmermans, Rian A H, den Besten, Heidy M W, Van Elburg, Ruurd M, Hettinga, Kasper, Kontopodi, Eva, Stahl, Bernd, van Goudoever, Johannes B, Boeren, Sjef, Timmermans, Rian A H, den Besten, Heidy M W, Van Elburg, Ruurd M, and Hettinga, Kasper

Abstract

Holder pasteurization (HoP) is the current recommended treatment for donor human milk. Although this method inactivates microbial contaminants, it also negatively affects various milk components. High-pressure processing (HPP, 400, 500, and 600 MPa), ultraviolet-C irradiation (UV-C, 2,430, 3,645, and 4,863 J/L) and thermoultrasonication (TUS, 1,080 and 1,620 kJ/L) were investigated as alternatives to thermal pasteurization (HoP). We assessed the effects of these methods on microbiological safety, and on concentration and functionality of immunoglobulin A, lactoferrin, lysozyme and bile salt-stimulated lipase, with LC-MS/MS-based proteomics and activity assays. HoP, HPP, TUS, and UV-C at 4863 J/L, achieved >5-log 10 microbial reduction. Native protein levels and functionality showed the highest reduction following HoP, while no significant reduction was found after less intense HPP and all UV-C treatments. Immunoglobulin A, lactoferrin, and lysozyme contents were also preserved after low intensity TUS, but bile salt-stimulated lipase activity was significantly reduced. This study demonstrated that HPP and UV-C may be considered as suitable alternatives to HoP, since they were able to ensure sufficient microbial inactivation while at the same time better preserving the bioactive components of donor human milk. In summary, our results provide valuable insights regarding the evaluation and selection of suitable processing methods for donor human milk treatment, which may replace HoP in the future.

Published
2022

3. Effects of High-Pressure Processing, UV-C Irradiation and Thermoultrasonication on Donor Human Milk Safety and Quality

Author

Afd Chemical Biology and Drug Discovery, Chemical Biology and Drug Discovery, Kontopodi, Eva, Stahl, Bernd, van Goudoever, Johannes B, Boeren, Sjef, Timmermans, Rian A H, den Besten, Heidy M W, Van Elburg, Ruurd M, Hettinga, Kasper, Afd Chemical Biology and Drug Discovery, Chemical Biology and Drug Discovery, Kontopodi, Eva, Stahl, Bernd, van Goudoever, Johannes B, Boeren, Sjef, Timmermans, Rian A H, den Besten, Heidy M W, Van Elburg, Ruurd M, and Hettinga, Kasper

Published
2022

5. Human milk from previously covid-19-infected mothers: The effect of pasteurization on specific antibodies and neutralization capacity

Author

van Keulen, Britt J, Romijn, Michelle, Bondt, Albert, Dingess, Kelly A, Kontopodi, Eva, van der Straten, Karlijn, den Boer, Maurits A, Burger, Judith A, Poniman, Meliawati, Bosch, Berend J, Brouwer, Philip J M, de Groot, Christianne J M, Hoek, Max, Li, Wentao, Pajkrt, Dasja, Sanders, Rogier W, Schoonderwoerd, Anne, Tamara, Sem, Timmermans, Rian A H, Vidarsson, Gestur, Stittelaar, Koert J, Rispens, Theo T, Hettinga, Kasper A, van Gils, Marit J, Heck, Albert J R, van Goudoever, Johannes B, Sub Biomol.Mass Spectrometry & Proteom., Afd Biomol.Mass Spect. and Proteomics, Virologie, dI&I I&I-1, Biomolecular Mass Spectrometry and Proteomics, Graduate School, Medical Microbiology and Infection Prevention, AII - Infectious diseases, Paediatric Infectious Diseases / Rheumatology / Immunology, ARD - Amsterdam Reproduction and Development, Landsteiner Laboratory, Neonatology, AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, Pediatric surgery, Obstetrics and gynaecology, Amsterdam Reproduction & Development (AR&D), AGEM - Endocrinology, metabolism and nutrition, ACS - Diabetes & metabolism, Sub Biomol.Mass Spectrometry & Proteom., Afd Biomol.Mass Spect. and Proteomics, Virologie, dI&I I&I-1, and Biomolecular Mass Spectrometry and Proteomics

Subjects
Adult, 0301 basic medicine, Epidemiology, Bioinformatica & Diermodellen, Breastfeeding, Pasteurization, Immunoglobulins, Biology, Antibodies, Viral, medicine.disease_cause, Article, Neutralization, Microbiology, law.invention, 03 medical and health sciences, law, Lactation, Bio-informatics & Animal models, medicine, Humans, TX341-641, Epidemiology, Bio-informatics & Animal models, Coronavirus, VLAG, Epidemiologie, 030109 nutrition & dietetics, Nutrition and Dietetics, Milk, Human, SARS-CoV-2, Nutrition. Foods and food supply, Outbreak, COVID-19, Antibodies, Neutralizing, 030104 developmental biology, medicine.anatomical_structure, Food Quality and Design, Immunization, Epidemiologie, Bioinformatica & Diermodellen, biology.protein, Food Technology, Female, Antibody, Food Science
Abstract

Background: Since the outbreak of coronavirus disease 2019 (COVID-19), many put their hopes in the rapid availability of effective immunizations. Human milk, containing antibodies against syndrome coronavirus 2 (SARS-CoV-2), may serve as means of protection through passive immunization. We aimed to determine the presence and pseudovirus neutralization capacity of SARS-CoV-2 specific IgA in human milk of mothers who recovered from COVID-19, and the effect of pasteurization on these antibodies. Methods: This prospective case control study included lactating mothers, recovered from (suspected) COVID-19 and healthy controls. Human milk and serum samples were collected. To assess the presence of SARS-CoV-2 antibodies we used multiple complementary assays, namely ELISA with the SARS-CoV-2 spike protein (specific for IgA and IgG), receptor binding domain (RBD) and nucleocapsid (N) protein for IgG in serum, and bridging ELISA with the SARS-CoV-2 RBD and N protein for specific Ig (IgG, IgM and IgA in human milk and serum). To assess the effect of pasteurization, human milk was exposed to Holder (HoP) and High Pressure Pasteurization (HPP). Results: Human milk contained abundant SARS-CoV-2 antibodies in 83% of the proven cases and in 67% of the suspected cases. Unpasteurized milk with and without these antibodies was found to be capable of neutralizing a pseudovirus of SARS-CoV-2 in (97% and 85% of the samples respectively). After pasteurization, total IgA antibody levels were affected by HoP, while SARS-CoV-2 specific antibody levels were affected by HPP. Pseudovirus neutralizing capacity of the human milk samples was only retained with the HPP approach. No correlation was observed between milk antibody levels and neutralization capacity. Conclusions: Human milk from recovered COVID-19-infected mothers contains SARS-CoV-2 specific antibodies which maintained neutralization capacity after HPP. All together this may represent a safe and effective immunization strategy after HPP.

Published
2021
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6. Human Milk from Previously COVID-19-Infected Mothers: The Effect of Pasteurization on Specific Antibodies and Neutralization Capacity

Author

Sub Biomol.Mass Spectrometry & Proteom., Afd Biomol.Mass Spect. and Proteomics, Virologie, dI&I I&I-1, Biomolecular Mass Spectrometry and Proteomics, van Keulen, Britt J, Romijn, Michelle, Bondt, Albert, Dingess, Kelly A, Kontopodi, Eva, van der Straten, Karlijn, den Boer, Maurits A, Burger, Judith A, Poniman, Meliawati, Bosch, Berend J, Brouwer, Philip J M, de Groot, Christianne J M, Hoek, Max, Li, Wentao, Pajkrt, Dasja, Sanders, Rogier W, Schoonderwoerd, Anne, Tamara, Sem, Timmermans, Rian A H, Vidarsson, Gestur, Stittelaar, Koert J, Rispens, Theo T, Hettinga, Kasper A, van Gils, Marit J, Heck, Albert J R, van Goudoever, Johannes B, Sub Biomol.Mass Spectrometry & Proteom., Afd Biomol.Mass Spect. and Proteomics, Virologie, dI&I I&I-1, Biomolecular Mass Spectrometry and Proteomics, van Keulen, Britt J, Romijn, Michelle, Bondt, Albert, Dingess, Kelly A, Kontopodi, Eva, van der Straten, Karlijn, den Boer, Maurits A, Burger, Judith A, Poniman, Meliawati, Bosch, Berend J, Brouwer, Philip J M, de Groot, Christianne J M, Hoek, Max, Li, Wentao, Pajkrt, Dasja, Sanders, Rogier W, Schoonderwoerd, Anne, Tamara, Sem, Timmermans, Rian A H, Vidarsson, Gestur, Stittelaar, Koert J, Rispens, Theo T, Hettinga, Kasper A, van Gils, Marit J, Heck, Albert J R, and van Goudoever, Johannes B

Published
2021

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