1. The Stem of Vesicular Stomatitis Virus G Can Be Replaced With the HIV-1 Env Membrane-Proximal External Region Without Loss of G Function or Membrane-Proximal External Region Antigenic Properties
- Author
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Ross W. B. Lindsay, Ivo C. Lorenz, Jaap Willem Back, Gavin Morrow, Christy Jurgens, Sanjay Phogat, Hanh T. Nguyen, Joanne DeStefano, Heather Arendt, Christopher L. Parks, Kevin J. Wright, Maoli Yuan, M Kemelman, Simon Hoffenberg, and Timothy J. Zamb
- Subjects
Immunogen ,medicine.drug_class ,viruses ,Immunology ,HIV Antibodies ,Biology ,Virus Replication ,Monoclonal antibody ,Epitope ,Virus ,Viral Envelope Proteins ,Antigen ,Virology ,medicine ,Animals ,Membrane Glycoproteins ,env Gene Products, Human Immunodeficiency Virus ,Vesiculovirus ,biology.organism_classification ,Antibodies, Neutralizing ,Molecular biology ,Recombinant Proteins ,Infectious Diseases ,Viral replication ,Vesicular stomatitis virus ,biology.protein ,Female ,Rabbits ,Antibody - Abstract
The structure of the HIV-1 envelope membrane-proximal external region (MPER) is influenced by its association with the lipid bilayer on the surface of virus particles and infected cells. To develop a replicating vaccine vector displaying MPER sequences in association with membrane, Env epitopes recognized by the broadly neutralizing antibodies 2F5, 4E10, or both were grafted into the membrane-proximal stem region of the vesicular stomatitis virus (VSV) glycoprotein (G). VSV encoding functional G-MPER chimeras based on G from the Indiana or New Jersey serotype propagated efficiently, although grafting of both epitopes (G-2F5-4E10) modestly reduced replication and resulted in the acquisition of one to two adaptive mutations in the grafted MPER sequence. Monoclonal antibodies 2F5 and 4E10 efficiently neutralized VSV G-MPER vectors and bound to virus particles in solution, indicating that the epitopes were accessible in the preattachment form of the G-MPER chimeras. Overall, our results showed that the HIV Env MPER could functionally substitute for the VSV G-stem region implying that both perform similar functions even though they are from unrelated viruses. Furthermore, we found that the MPER sequence grafts induced low but detectable MPER-specific antibody responses in rabbits vaccinated with live VSV, although additional vector and immunogen modifications or use of a heterologous prime-boost vaccination regimen will be required to increase the magnitude of the immune response.
- Published
- 2014