2,552 results on '"Ting JIANG"'
Search Results
2. Artificial intelligence-driven rational design of ionizable lipids for mRNA delivery
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Wei Wang, Kepan Chen, Ting Jiang, Yiyang Wu, Zheng Wu, Hang Ying, Hang Yu, Jing Lu, Jinzhong Lin, and Defang Ouyang
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Science - Abstract
Abstract Lipid nanoparticles (LNPs) have proven effective in mRNA delivery, as evidenced by COVID-19 vaccines. Its key ingredient, ionizable lipids, is traditionally optimized by inefficient and costly experimental screening. This study leverages artificial intelligence (AI) and virtual screening to facilitate the rational design of ionizable lipids by predicting two key properties of LNPs, apparent pKa and mRNA delivery efficiency. Nearly 20 million ionizable lipids were evaluated through two iterations of AI-driven generation and screening, yielding three and six new molecules, respectively. In mouse test validation, one lipid from the initial iteration, featuring a benzene ring, demonstrated performance comparable to the control DLin-MC3-DMA (MC3). Notably, all six lipids from the second iteration equaled or outperformed MC3, with one exhibiting efficacy akin to a superior control lipid SM-102. Furthermore, the AI model is interpretable in structure-activity relationships.
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- 2024
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3. Evaluating the Anticancer Potential of Ornithogalum saundersiae Saponins on Hepatocellular Carcinoma: A Transcriptome Sequencing Method
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Bo XU, Ting JIANG, Wangde JIN, and Xinglin JIN
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tiger's eye saponin ,transcriptome sequencing ,hep3b cells ,differentially expressed genes ,Food processing and manufacture ,TP368-456 - Abstract
To investigate the crucial pathways and differentially expressed genes following the action of Ornithogalum saundersiae Saponins (OSW-1) on liver cancer cells (HEP3B), this study utilized transcriptome sequencing technology to examine the gene expression data of HEP3B cells treated with OSW-1. By precisely analyzing the transcripts from the measured sequence information, differentially expressed genes related to cell survival were screened to identify core genes. Furthermore, experiments were conducted to verify whether OSW-1 could treat hepatocellular carcinoma by regulating the core targets. Results showed that the MTT assay revealed a significant reduction in HEP3B cell activity following OSW-1 treatment, which was dependent on the concentration. A total of 1381 genes showed significant differences in expression as a result of transcriptome sequencing, with a majority being involved in the TNF signaling pathway. Additionally, immunofluorescence and Western blot analysis indicated that the levels of p-PI3K, p-AKT, and p-NF-κB proteins were reduced in HEP3B cells after treatment with OSW-1. In conclusion, OSW-1 may suppress inflammation and reduce HEP3B cell activity by modulating inflammatory signaling pathways, suggesting its potential as a drug for liver cancer treatment and providing an experimental foundation for the development of healthy foods aimed at liver protection.
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- 2024
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4. Association between obesity measurement indexes and symptomatic knee osteoarthritis among the Chinese population: analysis from a nationwide longitudinal study
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Hao Lv, Yan Wang, Ge Zhang, Xingyu Wang, Zhimu Hu, Qingsong Chu, Yao Zhou, Yuxiang Yang, Ting Jiang, and Jiuxiang Wang
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Knee osteoarthritis ,Obesity measurement indexes ,CHARLS ,Cross-sectional survey ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Abstract Background The current literature lacks robust clinical data and evidence delineating the relationship between obesity measurement indexes and knee osteoarthritis (KOA). Consequently, this investigation seeks to elucidate the potential link between obesity measurement indexes and KOA among Chinese adults in a nationally representative study. Methods Firstly, this research performed an observational study in the China Health and Retirement Longitudinal Study (CHARLS). The variables were extracted from interviews and compared between KOA and non-KOA participants. The relationship between obesity measurement indexes and KOA was analyzed by multivariate logistic regression. Restricted cubic spline (RCS) regression tests the nonlinearity of the relationship between obesity measurement indexes and KOA. Subgroup analyses were performed by sex to verify the robustness of the findings. Results In this cross-sectional analysis, we found a positive association between obesity measurement indexes and KOA. These results did not change on multiple imputations(BMI: OR = 1.02, 95% CI, 1.01–1.04, P
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- 2024
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5. Characteristics and main controlling factors for the development of shale micro pore-fracture in Tiemulike Formation, Yining Basin
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Yangwei Feng, Yan Ren, Wei Sun, and Ting Jiang
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Shale gas ,Micro pore ,Controlling factors ,Thermal evolution ,Brittle minerals ,Medicine ,Science - Abstract
Abstract Research on the type, size, structure, and other characteristics of shale micro pore-fracture and their genesis is one of the core index for Shale gas study. Based on systematically collected shale samples from outcrop profiles and well cores, the experiments of thin-section observation, scanning electron microscopy, energy-dispersive X-ray spectroscopy, whole-rock analysis, rock–eval pyrolysis and basin simulation analysis were performed to study the micro pore-fracture characteristics and its main controlling factors for the development of shale pores in Tiemulike Formation in Yining Basin. The results show that four types of micro pore-fractures were identified: organic hydrocarbon-generating micro pores, granular dissolved micro pores, intergranular micro pores, and micro-fracture. The development of micro pores in shales is influenced by the internal material composition of the shale reservoir and external temperature conditions. The high organic carbon content with a high degree of thermal evolution led to the development of numerous micro pores, and the main micro pores were produced by shrinking the hydrocarbon generation volume due to the thermal evolution of organic matter. The development of micro-fractures was found to be favoured by the high content of brittle minerals and overpressure in the formation.
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- 2024
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6. Symptom network differences in school adjustment and anxiety-depression-stress in adolescents: a gender-based perspective
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Sufeila Shalayiding, Weicui Meng, Xiaoting Wang, Bahedana Sailike, and Ting Jiang
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Adolescent ,Anxiety ,Depression ,Psychological stress ,Gender differences ,Public aspects of medicine ,RA1-1270 - Abstract
Abstract Objective To investigate the characteristics of adolescents’ school adjustment and their associations with anxiety, depression, and stress, respectively, as well as their differences by gender. Methods A convenience sampling method was used to measure 3,922 secondary school students from 10 secondary schools in five locations in the Xinjiang Uygur Autonomous Region using the Anxiety-Depression-Stress Scale and the School Adjustment Scale, and latent profile analysis was used to identify the subjects’ school adjustment categories, and network analysis was subsequently used to explore the relationship between different school adjustment categories and anxiety, depression, and stress, respectively, as well as their gender characteristics. Results In the symptom network of adolescents at risk for school maladjustment, “school emotions and attitudes” was the core symptom (Expected Impact Index: 0.86); in the co-morbidity network of school adjustment and anxiety, depression, and stress in adolescents at risk for school maladjustment, the core symptom was “uneasiness” (Expected Impact Index: 0.86); and “stress” (Expected Impact Index: 0.86) was the core symptom. “(EI: 1.12), “difficulty relaxing” (EI: 1.14) in males, and “depression” (EI: 1.06) in females, all with significant gender differences. Significant gender differences were found. Conclusion Adolescents’ school adjustment was strongly associated with symptoms of anxiety, depression and stress, with significant gender differences in the structure of the network of co-morbid symptoms.
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- 2024
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7. EZH1/2 plays critical roles in oocyte meiosis prophase I in mice
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Ting Jiang, Chengxiu Zhang, Xinjing Cao, Yingpu Tian, Han Cai, Shuangbo Kong, Jinhua Lu, Haibin Wang, and Zhongxian Lu
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EZH1 ,EZH2 ,Oocyte meiosis ,DSBs repair ,Embryonic ovary ,Biology (General) ,QH301-705.5 - Abstract
Abstract Backgroud abnormalities or defects in oocyte meiosis can result in decreased oocyte quality, reduced ovarian reserve, and female diseases. However, the mechanisms of oocyte meiosis remain largely unknown, especially epigenetic regulation. Here, we explored the role of EZH1/2 (histone methyltransferase of H3K27) in mouse oocyte meiosis by inhibiting its activity and deleting its gene. Results with embryonic ovary cultured in vitro, EZH1/2 was demonstrated to be essential for oocyte development during meiosis prophase I in mice. Activity inhibition or gene knockout of EZH1/2 resulted in cell apoptosis and a reduction in oocyte numbers within embryonic ovaries. By observing the expression of some meiotic marker protein (γ-H2AX, diplotene stage marker MSY2 and synapsis complex protein SCP1), we found that function deficiency of EZH1/2 resulted in failure of DNA double-strand breaks (DSBs) repair and break of meiotic progression in fetal mouse ovaries. Moreover, Ezh1/2 deficiency led to the suppression of ATM (Ataxia Telangiectasia Mutated kinase) phosphorylation and a decrease in the expression of key DNA repair proteins Hormad1, Mre11, Rad50, and Nbs1 in fetal mouse ovaries, underscoring the enzyme’s pivotal role in initiating DNA repair. RNA-seq analysis revealed that Ezh1/2-deletion induced abnormal expression of multiple genes involved into several function of oocyte development in embryonic ovaries. Knockout of Ezh1/2 in ovaries also affected the levels of H3K9me3 and H4K20me2, as well as the expression of their target genes L3mbtl4 and Fbxo44. Conclusions our study demonstrated that EZH1/2 plays a role in the DSBs repair in oocyte meiosis prophase I via multiple mechanisms and offers new insights into the physiological regulatory role of histone modification in fetal oocyte guardianship and female fertility.
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- 2024
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8. CEP112 coordinates translational regulation of essential fertility genes during spermiogenesis through phase separation in humans and mice
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Xueguang Zhang, Gelin Huang, Ting Jiang, Lanlan Meng, Tongtong Li, Guohui Zhang, Nan Wu, Xinyi Chen, Bingwang Zhao, Nana Li, Sixian Wu, Junceng Guo, Rui Zheng, Zhiliang Ji, Zhigang Xu, Zhenbo Wang, Dong Deng, Yueqiu Tan, and Wenming Xu
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Science - Abstract
Abstract Spermiogenesis, the complex transformation of haploid spermatids into mature spermatozoa, relies on precise spatiotemporal regulation of gene expression at the post-transcriptional level. The mechanisms underlying this critical process remain incompletely understood. Here, we identify centrosomal protein 112 (CEP112) as an essential regulator of mRNA translation during this critical developmental process. Mutations in CEP112 are discovered in oligoasthenoteratospermic patients, and Cep112-deficient male mice recapitulate key phenotypes of human asthenoteratozoospermia. CEP112 localizes to the neck and atypical centrioles of mature sperm and forms RNA granules during spermiogenesis, enriching target mRNAs such as Fsip2, Cfap61, and Cfap74. Through multi-omics analyses and the TRICK reporter assay, we demonstrate that CEP112 orchestrates the translation of target mRNAs. Co-immunoprecipitation and mass spectrometry identify CEP112’s interactions with translation-related proteins, including hnRNPA2B1, EEF1A1, and EIF4A1. In vitro, CEP112 undergoes liquid-liquid phase separation, forming condensates that recruit essential proteins and mRNAs. Moreover, variants in patient-derived CEP112 disrupt phase separation and impair translation efficiency. Our results suggest that CEP112 mediates the assembly of RNA granules through liquid-liquid phase separation to control the post-transcriptional expression of fertility-related genes. This study not only clarifies CEP112’s role in spermatogenesis but also highlights the role of phase separation in translational regulation, providing insights into male infertility and suggesting potential therapeutic targets.
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- 2024
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9. ARID1 is required to regulate and reinforce H3K9me2 in sperm cells in Arabidopsis
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Lei Li, Huaihao Yang, Yi Zhao, Qianqian Hu, Xiaotuo Zhang, Ting Jiang, Hua Jiang, and Binglian Zheng
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Science - Abstract
Abstract Heterochromatin de-condensation in companion gametic cells is conserved in both plants and animals. In plants, microspore undergoes asymmetric pollen mitosis (PMI) to produce a vegetative cell (VC) and a generative cell (GC). Subsequently, the GC undergoes pollen mitosis (PMII) to produce two sperm cells (SC). Consistent with heterochromatin de-condensation in the VC, H3K9me2, a heterochromatin mark, is barely detected in VC. However, how H3K9me2 is differentially regulated during pollen mitosis remains unclear. Here, we show that H3K9me2 is gradually evicted from the VC since PMI but remain unchanged in the GC and SC. ARID1, a pollen-specific transcription factor that facilitates PMII, promotes H3K9me2 maintenance in the GC/SC but slows down its eviction in the VC. The genomic targets of ARID1 mostly overlaps with H3K9me2 loci, and ARID1 recruits H3K9 methyltransferase SUVH6. Our results uncover that differential pattern of H3K9me2 between two cell types is regulated by ARID1 during pollen mitosis.
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- 2024
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10. Design and Performance Optimization of a Portable VOCs Mass Spectrometer System
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Zhen-yu CHEN, Bao-kun YANG, Ting JIANG, and Wei XU
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portable mass spectrometer ,ion trap ,discontinuous sampling ,volatile organic compounds (vocs) ,Chemistry ,QD1-999 - Abstract
Due to their high reactivity, volatile organic compounds (VOCs) can engage in physical and chemical reactions with particulate matter in the atmosphere, leading to air pollution. Extended exposure to VOCs may result in various health concerns, including respiratory system problems, neurological effects, skin and eye irritation, and an increased risk of certain cancers. Traditional benchtop mass spectrometers possess the advantages of high sensitivity and rapid analysis capabilities, but the large size typically confines their applications to professional laboratories. To overcome this limitation, portable mass spectrometers are emerging as a new tool for in-situ analysis. In this study, a portable mass spectrometer based on discontinuous atmosphere pressure interface was developed, which combined with a sub-vacuum plasma ionization source. Ion trap, known for its small size and adequate performance, was adopted in this miniaturized mass spectrometer. This portable mass spectrometer features a total weight of about 4.6 kg, with the dimensions of 217 mm×162 mm×106 mm, and operates with a power consumption under 150 W. On this design, a fully metallic solenoid valve was selected as the core component to limit atmospheric pressure, eliminating the need for the rubber tubing used in traditional pinch valves. Compared to traditional pinch valves, this solenoid valve ensures a more stable airflow during experiments. As a result, the repeatability and environmental adaptability of the portable mass spectrometer were improved. A certain delay marked as radio freqency (RF) delay was introduced between the injection and cooling periods, and the RF-delay time was optimized. Frequency scanning mode and amplitude scanning mode were tested in parallel. Despite of a trade-off in size, amplitude scanning offered improved resolution, enabling the differentiation of ions with close mass to charge ratios. After systematic optimization, satisfactory performances in mass resolution and sensitivity were achieved. The mass resolution of the mass spectrometer for dimethyl carbonate was improved to 0.2 u (FWHM), and a sensitivity of 60 μg/m3 for naphthalene was achieved. The capability of mixed VOCs was also validated using dimethyl carbonate and trimethyl phosphate. This mass spectrometer developed in this study, characterized by its high portability and satisfactory performance, is expected to hold great potential for applications in VOCs analysis.
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- 2024
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11. Apigenin as an emerging hepatoprotective agent: current status and future perspectives
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Cheng Wang, Xiaoli Feng, Wen Li, Li Chen, Xinming Wang, Yimiao Lan, Rong Tang, Ting Jiang, Lingli Zheng, and Gang Liu
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apigenin ,liver disease ,hepatoprotection ,toxicity ,pharmacokinetics ,new formulations ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Apigenin (C15H10O5, API) is a natural flavonoid widely found in vegetables, fruits, and plants such as celery, oranges, and chamomile. In recent years, API has attracted considerable attention as a dietary supplement due to its low toxicity, non-mutagenic properties and remarkable therapeutic efficacy in various diseases. In particular, evidence from a large number of preclinical studies suggests that API has promising effects in the prevention and treatment of a variety of liver diseases, including multifactorial liver injury, non-alcoholic fatty liver disease/non-alcoholic steatohepatitis, liver fibrosis and liver cancer. This paper provides a comprehensive review of the progress of research into the therapeutic applications of API in liver diseases as of August 2024, based on literature retrieved from databases such as Web of Science, PubMed, CNKI, Google Scholar and ScienceDirect. The hepatoprotective effects of API involve multiple molecular mechanisms, including inhibition of inflammation, alleviation of hepatic oxidative stress, amelioration of insulin resistance, promotion of fatty acid oxidation, inhibition of liver cancer cell proliferation and differentiation, and induction of tumour cell apoptosis. More importantly, signaling pathways such as Nrf2, NF-κB, PI3K/Akt/mTOR, NLRP3, Wnt/β-catenin, TGF-β1/Smad3, AMPK/SREBP, PPARα/γ, MAPKs, and Caspases are identified as key targets through which API exerts its beneficial effects in various liver diseases. Studies on its toxicity and pharmacokinetics indicate that API has low toxicity, is slowly metabolized and excreted in vivo, and has low oral bioavailability. In addition, the paper summarises and discusses the sources, physicochemical properties, new dosage forms, and current challenges and opportunities of API, with the aim of providing direction and rationale for the further development and clinical application of API in the food, pharmaceutical and nutraceutical fields.
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- 2024
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12. Integration analysis of microRNAs as potential biomarkers in early-stage lung adenocarcinoma: the diagnostic and therapeutic significance of miR-183-3p
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Guodong Huang, Yuxia Liu, Lisha Li, Bing Li, Ting Jiang, Yufeng Cao, Xiaoping Yang, Xinning Liu, Honglin Qu, Shitao Li, and Xin Zheng
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lung adenocarcinoma ,microRNA sequencing ,biomarkers ,miR-183-3p ,SESN1 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
IntroductionLung adenocarcinoma (LUAD) poses a significant therapeutic challenge, primarily due to delayed diagnosis and the limited efficacy of existing treatments.MethodsTo understand the pathogenesis and identify diagnostic biomarkers for LUAD in the early stage, we investigated differential miRNA expression in 33 stage I LUAD patients between tumor and matched paracancerous tissues by Illumina Sequencing. Target genes of differentially expressed miRNAs were predicted using TargetScan and miRDB databases and further analyzed by GO and KEGG pathway enrichment analysis. The miRNAs expression results were verified using qRT-PCR. Additionally, we evaluated the clinical significance of miRNAs by the TCGA database. miR-183-3p was chosen for subsequent biological functional studies by cell proliferation assays, cell migration and cell invasion assays, cell apoptosis and cell cycle assays in LUAD cells. The clinical relevance target genes of miR-183-3p were predicted by TargetScan databases and bioinformatics assays. Gene-specific experimental validation was performed using qRT-PCR, western blotting and luciferase reporter assays.ResultsWe identified 36 differentially expressed miRNAs between LUAD tissues and matched paracancerous tissues. Target genes for these miRNAs revealed associations with processes and pathways such as RNA biosynthesis, intracellular signaling, protein transport, and the Ras, MAPK, and PI3K-AKT pathways. The qRT-PCR results were in alignment with the sequencing data for 19 out of these 21 miRNAs which not yet implicated in LUAD, 13 were up-regulated, 6 were down-regulated. The clinical relevance assays showed that 5 up-regulated miRNAs have diagnostic value for LUAD. miR-183-3p showed significant advantages in the result of sequencing, qRT-PCR, and clinical relevance assay. Biological functional assays showed that miR-183-3p emerged as a key regulator, promoting LUAD cell proliferation, decreasing apoptosis, and augmenting migration and invasion capabilities. The clinical relevance assays and experimental validation showed SESN1 as a clinical significance target of miR-183-3p.DiscussionOur study lays the foundation for investigating miRNAs with diagnostic significance in early-stage LUAD, pointing out that inhibition of miR-183-3p may serve as a novel therapeutic in LUAD.
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- 2024
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13. A type II secreted subtilase from commensal rhizobacteria cleaves immune elicitor peptides and suppresses flg22-induced immune activation
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Samuel Eastman, Ting Jiang, Kaeli Ficco, Chao Liao, Britley Jones, Sarina Wen, Yvette Olivas Biddle, Aya Eyceoz, Ilya Yatsishin, Todd A. Naumann, and Jonathan M. Conway
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CP: Plants ,CP: Microbiology ,Biology (General) ,QH301-705.5 - Abstract
Summary: Plant roots grow in association with a community of microorganisms collectively known as the rhizosphere microbiome. Immune activation in response to elicitors like the flagellin-derived epitope flg22 restricts bacteria on plant roots but also inhibits plant growth. Some commensal root-associated bacteria are capable of suppressing the plant immune response to elicitors. In this study, we investigated the ability of 165 root-associated bacteria to suppress flg22-induced immune activation and growth restriction. We demonstrate that a type II secreted subtilase, which we term immunosuppressive subtilase A (IssA), from Dyella japonica strain MF79 cleaves the immune elicitor peptide flg22 and suppresses immune activation. IssA homologs are found in other plant-associated commensals, with particularly high conservation in the order Xanthomonadales. This represents a novel mechanism by which commensal microbes modulate flg22-induced immunity in the rhizosphere microbiome.
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- 2024
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14. Single-cell RNA sequencing reveals the changes of the pulmonary immune environment in rat after Siegesbeckia orientalis L. treatment
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Fan Zhang, Shu Gan, Jingjing Liao, Ting Jiang, Zhiqiang Shi, Xueying Fan, Hiu-Yee Kwan, Zhongqiu Liu, and Tao Su
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Therapeutics. Pharmacology ,RM1-950 - Published
- 2024
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15. Optical Brewster interfaces enabled object identification and 3D reconstruction
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Zhuolang Liao, Dingyu Xu, Zhaoxin Wen, Yong Zhang, Ting Jiang, and Zhaoming Luo
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Applied optics. Photonics ,TA1501-1820 - Abstract
Efficient and accurate object identification and 3D reconstruction are crucial for processing image information in visual imaging. Here, we propose a novel scheme for all-optical 2D contour identification and 3D reconstruction based on optical Brewster interfaces. It is revealed that 2D amplitude and phase contours for high-contrast and low-contrast objects can be identified, which is attributed to the 1D and 2D light fields manipulated by the photonic spin Hall and the Brewster effects. The 3D model can be reconstructed by rotating or slicing the high-contrast objects and by inverting the thickness of the low-contrast objects. The study potentially opens up opportunities in applications such as intelligent driving and microscopic imaging.
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- 2024
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16. Imaging Metabolic Flow of Water in Plants with Isotope‐Traced Stimulated Raman Scattering Microscopy
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Simin Bi, Jianpeng Ao, Ting Jiang, Xianmiao Zhu, Yimin Zhu, Weibing Yang, Binglian Zheng, and Minbiao Ji
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label‐free imaging ,plant science ,stimulated Raman scattering ,water metabolism ,Science - Abstract
Abstract Water plays a vital role in the life cycle of plants, participating in various critical biochemical reactions during both non‐photosynthetic and photosynthetic processes. Direct visualization of the metabolic activities of water in plants with high spatiotemporal resolution is essential to reveal the functional utilization of water. Here, stimulated Raman scattering (SRS) microscopy is applied to monitor the metabolic processes of deuterated water (D2O) in model plant Arabidopsis thaliana (A. thaliana). The work shows that in plants uptaking D2O/water solution, proton‐transfer from water to organic metabolites results in the formation of C‐D bonds in newly synthesized biomolecules (lipid, protein, and polysaccharides, etc.) that allow high‐resolution detection with SRS. Reversible metabolic pathways of oil‐starch conversion between seed germination and seed development processes are verified. Spatial heterogeneity of metabolic activities along the vertical axis of plants (root, stem, and tip meristem), as well as the radial distributions of secondary growth on the horizontal cross‐sections are quantified. Furthermore, metabolic flow of protons from plants to animals is visualized in aphids feeding on A. thaliana. Collectively, SRS microscopy has potential to trace a broad range of matter flows in plants, such as carbon storage and nutrition metabolism.
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- 2024
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17. Unraveling the ultrastructure and dynamics of autophagic vesicles: Insights from advanced imaging techniques
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Ting Jiang, Chaoye Ma, and Hao Chen
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autophagy ,ultrastructure ,volume electron microscope ,Biology (General) ,QH301-705.5 - Abstract
Abstract Autophagy, an intracellular self‐degradation process, is governed by a complex interplay of signaling pathways and interactions between proteins and organelles. Its fundamental purpose is to efficiently clear and recycle cellular components that are damaged or redundant. Central to this process are autophagic vesicles, specialized structures that encapsulate targeted cellular elements, playing a pivotal role in autophagy. Despite growing interest in the molecular components of autophagic machinery and their regulatory mechanisms, capturing the detailed ultrastructural dynamics of autophagosome formation continues to present significant challenges. However, recent advancements in microscopy, particularly in electron microscopy, have begun to illuminate the dynamic regulatory processes underpinning autophagy. This review endeavors to provide an exhaustive overview of contemporary research on the ultrastructure of autophagic processes. By synthesizing observations from diverse technological methodologies, this review seeks to deepen our understanding of the genesis of autophagic vesicles, their membrane origins, and the dynamic alterations that transpire during the autophagy process. The aim is to bridge gaps in current knowledge and foster a more comprehensive comprehension of this crucial cellular mechanism.
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- 2024
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18. Species diversity of fish at the Wuzhizhou Island, South China Sea, based on environmental DNA
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Chongzhao Wang, Zhenhua Ma, Kun Cao, Xin Wang, Rui Xi, Ting Jiang, Rui Yang, and Yingchun Xing
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marine ecosystem ,occurrence of fish taxon ,distri ,Biology (General) ,QH301-705.5 - Abstract
Wuzhizhou Island (WZZ) is located in Haitang Bay in the northern region of Sanya, Hainan Island. The sea area surrounding WZZ represents a typical tropical marine ecosystem, characterised by diverse and complex habitats. Therefore, there is a rich variety of marine fish species at WZZ. The marine ecosystem of WZZ was seriously destroyed initially in the 1970s-1980s and recovered in the 1990s, then constructed as the first national tropical marine ranch demonstration area of China in 2019. As fish is an important high trophic vertebrate in the marine ecosystem, understanding the composition and distribution of fish species could help us to recognise the status of the ecosystem of WZZ and supply scientific data for construction of the national marine ranch demonstration area. This study used eDNA technology to investigate the composition of fish community surrounding WZZ and provided a scientific basis for realising and protecting the marine ecosystem of the South China Sea.The WZZ is an offshore island in the South China Sea, harbouring abundant marine fish resources. Although previous research investigated fish species of WZZ, the data were, however, still incomplete due to limitation of sampling methods and survey seasons. In this study, we intended to take advantage of eDNA and supplement data of fish species at WZZ as much as possible. Based on eDNA, this study provided the data on 188 fish species (including nine undetermined species denoted by genus sp.) belonging to 17 orders, 63 families and 124 genera and they were the more comprehensive records of fish species surrounding WZZ. In addition, the information on Molecular Operational Taxonomic Units (MOTUs) for taxon identification was also provided, aiming to contribute to the establishment of a specific eDNA taxon database for fish of the South China Sea. This study included two datasets, which were occurrences of fish taxa at WZZ, as well as MOTUs sequences and geographical coordinate information of sampling sites. The “fish taxon occurrences” dataset presented records on taxonomic, distribution and habitat conditions of 188 fish species detected using eDNA, as well as the latitude and longitude information of the sampling sites, the "MOTUs information" dataset provided the MOTUs sequences, source of sequences, abundance of sequences for 188 fish species, also included the species matched in NCBI and the best NCBI BLAST sequence similarity.
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- 2024
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19. Recurrent neural network for predicting absence of heterozygosity from low pass WGS with ultra-low depth
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Fei Tang, Zhonghua Wang, Yan Sun, Linlin Fan, Yun Yang, Xueqin Guo, Yaoshen Wang, Saiying Yan, Zhihong Qiao, Yun Li, Ting Jiang, Xiaoli Wang, Jianfen Man, Lina Wang, Shunyao Wang, Huanhuan Peng, Zhiyu Peng, Xiaoyuan Xie, and Lijie Song
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AOH ,RNN ,LP-WGS ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background The absence of heterozygosity (AOH) is a kind of genomic change characterized by a long contiguous region of homozygous alleles in a chromosome, which may cause human genetic disorders. However, no method of low-pass whole genome sequencing (LP-WGS) has been reported for the detection of AOH in a low-pass setting of less than onefold. We developed a method, termed CNVseq-AOH, for predicting the absence of heterozygosity using LP-WGS with ultra-low sequencing data, which overcomes the sparse nature of typical LP-WGS data by combing population-based haplotype information, adjustable sliding windows, and recurrent neural network (RNN). We tested the feasibility of CNVseq-AOH for the detection of AOH in 409 cases (11 AOH regions for model training and 863 AOH regions for validation) from the 1000 Genomes Project (1KGP). AOH detection using CNVseq-AOH was also performed on 6 clinical cases with previously ascertained AOHs by whole exome sequencing (WES). Results Using SNP-based microarray results as reference (AOHs detected by CNVseq-AOH with at least a 50% overlap with the AOHs detected by chromosomal microarray analysis), 409 samples (863 AOH regions) in the 1KGP were used for concordant analysis. For 784 AOHs on autosomes and 79 AOHs on the X chromosome, CNVseq-AOH can predict AOHs with a concordant rate of 96.23% and 59.49% respectively based on the analysis of 0.1-fold LP-WGS data, which is far lower than the current standard in the field. Using 0.1-fold LP-WGS data, CNVseq-AOH revealed 5 additional AOHs (larger than 10 Mb in size) in the 409 samples. We further analyzed AOHs larger than 10 Mb, which is recommended for reporting the possibility of UPD. For the 291 AOH regions larger than 10 Mb, CNVseq-AOH can predict AOHs with a concordant rate of 99.66% with only 0.1-fold LP-WGS data. In the 6 clinical cases, CNVseq-AOH revealed all 15 known AOH regions. Conclusions Here we reported a method for analyzing LP-WGS data to accurately identify regions of AOH, which possesses great potential to improve genetic testing of AOH.
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- 2024
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20. Exposure to Titanium Dioxide Nanoparticles Leads to Specific Disorders of Spermatid Elongation via Multiple Metabolic Pathways in Drosophila Testes
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Xinmeng Cheng, Ting Jiang, Qiuru Huang, Li Ji, Jiaxin Li, Xiuwen Kong, Xiaoqi Zhu, Xuxin He, Xiaonan Deng, Tong Wu, Hao Yu, Yi Shi, Lin Liu, Xinyuan Zhao, Xiaorong Wang, Hao Chen, and Jun Yu
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Chemistry ,QD1-999 - Published
- 2024
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21. Bidirectional association identified between synovitis and knee and hand osteoarthritis: a general population-based studyResearch in context
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Ting Jiang, Qianlin Weng, Ke Liu, Hongyi He, Yuqing Zhang, Weiya Zhang, Michael Doherty, Junqing Xie, Tuo Yang, Jiatian Li, Zidan Yang, Qiu Chen, Huizhong Long, Yilun Wang, Jie Wei, Guanghua Lei, and Chao Zeng
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Osteoarthritis ,Synovitis ,Incidence ,General population ,Public aspects of medicine ,RA1-1270 - Abstract
Summary: Background: Synovitis has long been considered a common and modifiable inflammatory feature of osteoarthritis (OA), but current disease-modifying anti-inflammatory treatments appear ineffective in OA clinical trials. Elucidating the temporal relationship between synovitis and OA could provide insight into the role of synovitis in OA. Methods: We conducted a prospective cohort study based on the baseline and three-year follow-up data from the Xiangya Osteoarthritis (XO) Study. We assessed bidirectional associations between ultrasound-detected synovitis and radiographic and symptomatic OA at knee and hand sites using generalized estimating equations. Additionally, we performed bidirectional Mendelian randomization (MR) analyses to test these hypotheses utilising whole-genome sequencing data in the XO population. Age, sex, body mass index, smoking, alcohol consumption, educational level, physical activity, and joint injury history were adjusted for these analyses. Findings: A total of 2211, 2420, 2280, and 2600 participants were enrolled for analyses of radiographic knee OA (RKOA), symptomatic knee OA (SKOA), radiographic hand OA (RHOA) and symptomatic hand OA (SHOA), respectively. The baseline synovitis (i.e., with synovitis vs. without synovitis) was associated with the incident RKOA (76/277 vs. 557/3674 knees), SKOA (49/387 vs. 287/4213 knees), RHOA (171/358 vs. 686/3664 hands) and SHOA (35/689 vs. 76/4327 hands), with adjusted odds ratio (aORs) of 2.2 (95% CI 1.7–3.1), 2.0 (1.3–2.9), 3.4 (2.7–4.4), and 2.4 (1.5–3.8), respectively. The baseline RKOA (with OA vs. without OA: 409/1246 vs. 481/3758 knees), SKOA (200/576 vs. 675/4356 knees), RHOA (192/778 vs. 410/3723 hands), and SHOA (41/162 vs. 548/4285 hands) were also associated with the incident synovitis, with aORs of 3.4 (95% CI 2.9–4.1), 2.7 (2.1–3.4), 2.3 (1.8–2.9) and 1.9 (1.2–2.8), respectively. These bidirectional associations were stronger when more active synovitis was compared with the reference group (all P
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22. Laparoscopic anatomical partial splenectomy for extremely rare isolated splenic lymphangioma in an adult: a case report and literature review
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Ruizi Shi, Pei Yang, Yangjie Guo, Yiping Tang, Hua Luo, Chuan Qin, Ting Jiang, Yu Huang, Ziqing Gao, Xintao Zeng, and Jianjun Wang
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splenic benign tumor ,splenic lymphangioma ,laparoscopic surgery ,case report ,anatomical resection ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
BackgroundBenign tumors of the spleen are rare compared to those of other parenchymal organs, accounting for less than 0.007% of all tumors, and are often found incidentally. Splenolymphangiomas are much rarer, commonly occur in children, and tend to have multiple foci. Splenic lymphangiomas are rare in adults, and fewer than 20 adult patients with isolated splenic lymphangiomas have been reported. In this article, we report the case of a middle-aged female patient with isolated splenic lymphangioma who underwent laparoscopic anatomical hypophysectomy of the lower pole of the spleen. We also summarize the existing literature on splenic lymphangioma diagnosis and available treatment options.Case presentationA 58-year-old middle-aged woman was found to have a mass approximately 60 mm in diameter at the lower pole of the spleen during a health checkup that was not accompanied by other symptoms or examination abnormalities. After completing a preoperative examination with no contraindications to surgery, the patient underwent laparoscopic anatomical splenectomy of the lower extremity of the spleen. The patient recovered well without complications and was discharged from the hospital on the 7th postoperative day. Histopathological and immunohistochemical results confirmed the diagnosis of splenic lymphangioma. Prompt surgical intervention is safe and necessary when splenic lymphangiomas are large or associated with a risk of bleeding.ConclusionSplenic lymphangiomas are rare and require early surgical intervention in patients with large tumor diameters or those at risk of rupture and bleeding. After rigorous preoperative evaluation and preparation, laparoscopic anatomical partial splenectomy is safe and feasible for surgeons with experience in laparoscopic surgery.
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- 2024
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23. Health and economic impacts of Vaccae vaccination incorporating active case finding in India and South Africa: a modelling study
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Xun Zhuang, Bin Zhang, Ting Jiang, Jian Feng, Gang Qin, Xiao Zang, Jun-Jie Mao, Wan-Lu Yue, Pei-Yao Zhai, Jun-Feng Ma, and Wen-Long Ren
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Public aspects of medicine ,RA1-1270 - Abstract
Introduction Tuberculosis (TB) is a major global health issue, particularly, in India and South Africa. We aim to evaluate the potential of the new TB vaccine, Vaccae, to enhance TB control by integrating with active case finding (ACF) strategies in these regions.Methods Using age-structured dynamic models, we projected the epidemiological and economic outcomes of combining Vaccae vaccination with ACF over 27 years. In India, four age-targeted strategies were analysed: (1) adolescent (15 years), (2) adolescent and young adult (AYA, 15–34 years), (3) adult (18–55 years) and (4) elderly (60+ years). In South Africa, strategies were based on HIV status: (1) HIV-targeted, (2) non-HIV and (3) general population. The vaccine efficacy of Vaccae was set at 54.7%, priced at US$120 per course. Outcomes measured included reductions in TB incidence and mortality, prioritised based on budget, incremental cost-effectiveness ratios (ICER) and benefit-cost ratios (BCR).Results In India, the adult strategy could prevent 8.70 (95% UI: 6.86–11.13) million TB cases and 0.61 (0.39–0.91) million deaths, reducing incidence and mortality by 20.1% (18.7%–23.5%) and 19.0% (17.5%–22.5%), respectively. In South Africa, the general population strategy could avert 0.67 (0.45–0.99) million TB cases and 0.21 (0.15–0.28) million deaths, reducing incidence by 28.1% (26.1%–30.3%) and mortality by 27.0% (23.5%–30.0%). The vaccination budgets for the AYA strategy in India and the HIV-targeted strategy in South Africa were US$14.0 (10.14–23.29) billion and US$0.09 (0.06–0.15) billion, respectively. The ICERs were US$1082 (927–1426) and US$70 (53–123) per disability-adjusted life year averted, and the BCRs were 2.0 (1.6–2.4) and 176 (83.4–255.3), respectively. Thus, the AYA strategy in India and the HIV-targeted strategy in South Africa were the most favourable.Conclusion Integrating Vaccae vaccination with ACF could significantly enhance TB control in high-burden countries. Policymakers should consider these strategies, with further research needed to confirm the findings.
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- 2024
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24. Immunological perspectives on atherosclerotic plaque formation and progression
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Hui Pi, Guangliang Wang, Yu Wang, Ming Zhang, Qin He, Xilong Zheng, Kai Yin, Guojun Zhao, and Ting Jiang
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atherosclerosis ,plaque evolution ,immune response ,immune cell heterogeneity ,inflammatory microenvironment ,immunotherapy ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Atherosclerosis serves as the primary catalyst for numerous cardiovascular diseases. Growing evidence suggests that the immune response is involved in every stage of atherosclerotic plaque evolution. Rapid, but not specific, innate immune arms, including neutrophils, monocytes/macrophages, dendritic cells (DCs) and other innate immune cells, as well as pattern-recognition receptors and various inflammatory mediators, contribute to atherogenesis. The specific adaptive immune response, governed by T cells and B cells, antibodies, and immunomodulatory cytokines potently regulates disease activity and progression. In the inflammatory microenvironment, the heterogeneity of leukocyte subpopulations plays a very important regulatory role in plaque evolution. With advances in experimental techniques, the fine mechanisms of immune system involvement in atherosclerotic plaque evolution are becoming known. In this review, we examine the critical immune responses involved in atherosclerotic plaque evolution, in particular, looking at atherosclerosis from the perspective of evolutionary immunobiology. A comprehensive understanding of the interplay between plaque evolution and plaque immunity provides clues for strategically combating atherosclerosis.
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- 2024
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25. A review of local anesthetic-induced heart toxicity using human induced pluripotent stem cell-derived cardiomyocytes
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Ting Jiang, Chao Ma, Zitong Wang, and Yi Miao
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Local anesthetic cardiotoxicity ,Human induced pluripotent stem cells ,Cardiomyocytes ,Sodium channel dynamics ,Pharmacodynamics ,Biology (General) ,QH301-705.5 ,Medicine - Abstract
Local anesthetic (LA) cardiotoxicity is one of the main health problems in anesthesiology and pain management. This study reviewed the reported LA-induced cardiac toxicity types, risk factors, management, and mechanisms, with attention to the use of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) in heart toxicity research. Important scientific databases were searched to find relevant articles. We briefly assessed the reported cardiotoxic effects of different types of LA drugs, including ester- and amide-linked LA agents. Furthermore, cardiotoxic effects and clinical manifestations, strategies for preventing and managing LA-induced cardiotoxic effects, pharmacokinetics, pharmacodynamics, and sodium channel dynamics regarding individual variability and genetic influences were discussed in this review. The applications and importance of hiPSC-CMs cellular model for evaluating the cardiotoxic effects of LA drugs were discussed in detail. This review also explored hiPSC-CMs' potential in risk assessment, drug screening, and developing targeted therapies. The main mechanisms underlying LA-induced cardiotoxicity included perturbation in sodium channels, ROS production, and disorders in the immune system response due to the presence of LA drugs. Furthermore, drug-specific characteristics including pharmacokinetics and pharmacodynamics are important determinants after LA drug injection. In addition, individual patient factors such as age, comorbidities, and genetic variability emphasize the need for a personalized approach to mitigate risks and enhance patient safety. The strategies outlined for the prevention and management of LA cardiotoxicity underscore the importance of careful dosing, continuous monitoring, and the immediate availability of resuscitation equipment. This comprehensive review can be used to guide future investigations into better understanding LA cardiac toxicities and improving patient safety.
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- 2024
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26. Synergistic effect of ultrasound and reinforced electrical environment by bioinspired periosteum for enhanced osteogenesis via immunomodulation of macrophage polarization through Piezo1
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Ting Jiang, Fei Yu, Yuqi Zhou, Ruomei Li, Mengting Zheng, Yangyang Jiang, Zhenxia Li, Jun Pan, and Ningjuan Ouyang
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LIPUS ,Piezoelectric periosteum ,Electrical environment ,Macrophage ,Osteogenesis ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
The periosteum plays a vital role in repairing bone defects. Researchers have demonstrated the existence of electrical potential in the periosteum and native bone, indicating that electrical signals are essential for functional bone regeneration. However, the clinical use of external electrical treatments has been limited due to their inconvenience and inefficacy. As an alternative, low-intensity pulsed ultrasound (LIPUS) is a noninvasive form of physical therapy that enhances bone regeneration. Furthermore, the wireless activation of piezoelectric biomaterials through ultrasound stimulation would generate electric charges precisely at the defect area, compensating for the insufficiency of external electrical stimulation and potentially promoting bone regeneration through the synergistic effect of mechanical and electrical stimulation. However, the optimal integration of LIPUS with an appropriate piezoelectric periosteum is yet to be explored. Herein, the BaTiO3/multiwalled-carbon nanotubes/collagen (BMC) membranes have been fabricated, possessing physicochemical properties including improved surface hydrophilicity, enhanced mechanical performance, ideal piezoelectricity, and outstanding biocompatibility, all of which are conducive to bone regeneration. When combined with LIPUS, the endogenous electrical microenvironment of native bone was recreated. After that, the wireless-generated electrical signals, along with the mechanical signals induced by LIPUS, were transferred to macrophages and activated Ca2+ influx through Piezo1. Ultimately, the regenerative effect of the BMC membrane with LIPUS stimulation (BMC + L) was confirmed in a mouse cranial defect model. Together, this research presents a co-engineering strategy that involves fabricating a novel biomimetic periosteum and utilizing the synergistic effect of ultrasound to enhance bone regeneration, which is achieved through the reinforcement of the electrical environment and the immunomodulation of macrophage polarization.
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- 2024
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27. Immune cell-mediated features of atherosclerosis
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Tingting Liu, Yanjun Chen, Lianjie Hou, Yulu Yu, Dan Ma, Ting Jiang, and Guojun Zhao
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immune cells ,atherosclerosis ,targeted therapy ,cardiovascular diseases ,inflammation ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Atherosclerosis is a chronic inflammatory disease characterized by innate and adaptive immune responses, which seriously threatens human life and health. It is a primary cause of coronary heart disease, myocardial infarction, and peripheral vascular disease. Research has demonstrated that immune cells are fundamental to the development of atherosclerosis and chronic inflammation. Therefore, it is anticipated that immunotherapy targeting immune cells will be a novel technique in the management of atherosclerosis. This article reviews the growth of research on the regulatory role of immune cells in atherosclerosis and targeted therapy approaches. The purpose is to offer new therapeutic approaches for the control and treatment of cardiovascular illnesses caused by atherosclerosis.
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- 2024
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28. Comparison of anterior and posterior approach in the treatment of acute and chronic cervical spinal cord injury: a meta-analysis
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Yi Ding, Ning Li, Wenjing Hu, Wenkang Jiang, Qianmiao Zhu, Ting Jiang, and Huilin Cheng
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cervical spinal cord injury ,outcomes ,anterior approach ,posterior approach ,meta-analysis ,Surgery ,RD1-811 - Abstract
ObjectiveA cervical spinal cord injury (CSCI) is a traumatic catastrophe that often leads to neurological dysfunction. The optimal surgical procedure for the treatment of CSCI remains debatable. The aim of this meta-analysis is to compare the neurological outcomes, complications, and clinical factors between anterior and posterior approach in CSCI treatment.MethodsWe searched PubMed, Embase, Web of Science and Cochrane library from their inceptions to october 2023. Preoperative and postoperative Spinal Injury Association (ASIA) and Japanese Orthopedic Association (JOA) scores, and calculated recovery rates (RRs) were compared between the two strategies, and differences in complication rates, operation time, intraoperative blood loss and length of stay were also analyzed.ResultsA total of five studies containing 613 patients were included, with 320 patients undergoing the anterior approach and 293 patients undergoing the posterior approach. Four of the studies included were retrospective cohort studies of high quality as assessed by the Newcastle Ottawa Scale. Additionally, there was one randomized controlled trial evaluated with the Cochrane Risk of Bias tool. Although both anterior and posterior approaches effectively facilitate spinal decompression and promote good neurological recovery, there was no significant difference in the incidences of neurological dysfunction and complications or other clinical features between the two approaches.ConclusionThere is no evidence thus far supports one approach over the other. Large-scale randomized controlled studies are warranted to further distinguish these two methods.Systematic Review Registrationhttps://www.crd.york.ac.uk/, PROSPERO [CRD42023438831].
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- 2024
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29. In Situ Quantitative Monitoring of Adsorption from Aqueous Phase by UV–vis Spectroscopy: Implication for Understanding of Heterogeneous Processes
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Xu‐Dan Yang, Bo Gong, Wei Chen, Jie‐Jie Chen, Chen Qian, Rui Lu, Yuan Min, Ting Jiang, Liang Li, and Han‐Qing Yu
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adsorption ,computational chemistry ,heterogeneous reaction ,multivariate curve resolution ,UV–vis spectroscopy ,Science - Abstract
Abstract The development of in situ techniques to quantitatively characterize the heterogeneous reactions is essential for understanding physicochemical processes in aqueous phase. In this work, a new approach coupling in situ UV–vis spectroscopy with a two‐step algorithm strategy is developed to quantitatively monitor heterogeneous reactions in a compact closed‐loop incorporation. The algorithm involves the inverse adding‐doubling method for light scattering correction and the multivariate curve resolution‐alternating least squares (MCR‐ALS) method for spectral deconvolution. Innovatively, theoretical spectral simulations are employed to connect MCR‐ALS solutions with chemical molecular structural evolution without prior information for reference spectra. As a model case study, the aqueous adsorption kinetics of bisphenol A onto polyamide microparticles are successfully quantified in a one‐step UV–vis spectroscopic measurement. The practical applicability of this approach is confirmed by rapidly screening a superior adsorbent from commercial materials for antibiotic wastewater adsorption treatment. The demonstrated capabilities are expected to extend beyond monitoring adsorption systems to other heterogeneous reactions, significantly advancing UV–vis spectroscopic techniques toward practical integration into automated experimental platforms for probing aqueous chemical processes and beyond.
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- 2024
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30. Associations between hyperuricemia and ultrasound-detected knee synovial abnormalities in middle-aged and older population: a cross-sectional study
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Qianlin Weng, Ting Jiang, Weiya Zhang, Michael Doherty, Zidan Yang, and Jie Wei
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Hyperuricemia ,Synovial abnormality ,Knee ,Ultrasound ,Orthopedic surgery ,RD701-811 ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Abstract Objectives Knee synovial abnormalities, potentially treatment targets for knee pain and osteoarthritis, are common in middle-aged and older population, but its etiology remains unclear. We examined the associations between hyperuricemia and knee synovial abnormalities detected by ultrasound in a general population sample. Methods Participants aged ≥ 50 years were from a community-based observational study. Hyperuricemia was defined as serum urate (SU) level > 416 µmol/L in men and > 357 µmol/L in women. Ultrasound of both knees was performed to determine the presence of synovial abnormalities, i.e., synovial hypertrophy, effusion, or Power Doppler signal (PDS). We examined the relation of hyperuricemia to prevalence of knee synovial abnormalities and its laterality, and the dose-response relationships between SU levels and the prevalence of knee synovial abnormalities. Results In total, 3,405 participants were included in the analysis. Hyperuricemia was associated with higher prevalence of knee synovial abnormality (adjusted odds ratio [aOR] = 1.21, 95% confidence interval [CI]: 1.02 to 1.43), synovial hypertrophy (aOR = 1.33, 95% CI: 1.05 to 1.68), and effusion (aOR = 1.21, 95% CI: 1.02 to 1.44), respectively. There were dose-response relationships between SU levels and synovial abnormalities. Additionally, the hyperuricemia was more associated with prevalence of bilateral than with that of unilateral knee synovial abnormality, synovial hypertrophy, or effusion; however, no significant association was observed between hyperuricemia and PDS. Conclusion In this population-based study we found that hyperuricemia was associated with higher prevalence of knee synovial abnormality, synovial hypertrophy and effusion, suggesting that hyperuricemia may play a role in pathogenesis of knee synovial abnormalities.
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- 2024
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31. Efficacy and adverse reactions of peripheral add multifocal soft contact lenses in childhood myopia: a meta-analysis
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Desheng Song, Wen Qiu, Ting Jiang, Zhijun Chen, and Juan Chen
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Myopia ,Soft contact lens ,Children ,Meta-analysis ,Ophthalmology ,RE1-994 - Abstract
Abstract Objectives This study aims to compare the efficacy of peripheral add multifocal soft contact lenses (SCLs) (excluding bifocal SCLs) with single vision contact lenses or spectacles in controlling myopia progression. Method A comprehensive literature search was conducted in the Pubmed, EMBASE, Web of Science, and Cochrane Library databases until October 2023. The literature was thoroughly screened based on predetermined eligibility criteria. Pooled odds ratios (ORs) were calculated for dichotomous data and weighted mean differences (WMD) for continuous data. Results A total of 11 articles comprising 787 participants were included in this meta-analysis. Our pooled results demonstrated that the peripheral add multifocal SCLs groups exhibited significantly reduced refraction progression (MD = 0.20; 95%CI, 0.14 ∼ 0.27; P
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- 2024
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32. Breaking the speed limitation of wavemeter through spectra-space-time mapping
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Zheng Gao, Ting Jiang, Mingming Zhang, Yuxuan Xiong, Hao Wu, and Ming Tang
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speed ,wavemeters ,spectra ,Manufactures ,TS1-2301 ,Applied optics. Photonics ,TA1501-1820 - Abstract
Speckle patterns generated by the intermodal interference of multimode fibers enable accurate broadband wavelength measurements. However, the measurement speed is limited by the frame rate of the camera that captures the patterns. We propose a compact and cost-effective ultrafast wavemeter based on multimode and multicore fibers, which employs spectral–spatial–temporal mapping. The speckle patterns generated by multimode fibers enable spectral-to-spatial mapping, which is then sampled by a multicore fiber into a pulse sequence to implement spatial-to-temporal mapping. A high-speed single-pixel photodetector is employed to capture the pulse sequence, which is analysed using a multilayer perceptron to estimate the wavelength. The feasibility of the proposed wavelength estimation method is experimentally verified, achieving a measurement rate of 100 MHz with a resolution of 2.7 pm in a 1 nm operation bandwidth.
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- 2024
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33. Revealing the role of the gut microbiota in enhancing targeted therapy efficacy for lung adenocarcinoma
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Ting Jiang, Meng Zhang, Shaoyu Hao, Shi Huang, Xin Zheng, and Zheng Sun
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Lung adenocarcinoma ,Gut microbiota ,Mediation Analysis ,Targeted therapy ,Gefitinib efficacy ,Diseases of the blood and blood-forming organs ,RC633-647.5 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Lung adenocarcinoma (LUAD) is the leading cause of cancer-related death globally. Although the gut microbiota's role in the antitumor efficacy of many cancers has been revealed, its involvement in the response to gefitinib therapy for LUAD remains unclear. To fill this gap, we conducted a longitudinal study that profiled gut microbiota changes in PC-9 tumor-bearing mice under different treatments, including gefitinib monotherapy and combination therapies with probiotics, antibiotics, or Traditional Chinese Medicine (TCM). Our findings demonstrated that combining probiotics or TCM with gefitinib therapy outperformed gefitinib monotherapy, as evidenced by tumor volume, body weight, and tumor marker tests. By contrast, antibiotic intervention suppressed the antitumor efficacy of gefitinib. Notably, the temporal changes in gut microbiota were strongly correlated with the different treatments, prompting us to investigate whether there is a causal relationship between gut microbiota and the antitumor efficacy of gefitinib using Mediation Analysis (MA). Finally, our research revealed that thirteen mediators (Amplicon Sequence Variants, ASVs) regulate the antitumor effect of gefitinib, regardless of treatment. Our study provides robust evidence supporting the gut microbiota's significant and potentially causal role in mediating gefitinib treatment efficacy in mice. Our findings shed light on a novel strategy for antitumor drug development by targeting the gut microbiota.
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- 2024
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34. Examining the effect of training with a teaching for understanding framework on intravenous therapy administration’s knowledge, performance, and satisfaction of nursing students: a non-randomized controlled study
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Jing Huang, Xiaoyan Liu, Jing Xu, Li Ren, Lihui Liu, Ting Jiang, Menglu Huang, and Zhoupeng Wu
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Teaching for understanding framework ,Infusions ,Intravenous ,Students ,Nurses ,Teaching ,Nursing ,RT1-120 - Abstract
Abstract Background Nursing students require improvement in their intravenous infusion therapy management skills, yet traditional training models possess deficiencies. The Teaching for Understanding (TfU) Framework can enhance the teaching-learning process and support quality education. Therefore, utilizing TfU framework for training may promote the performance of nurses. Methods Utilizing a non-synchronized design, 102 nurses were recruited using a convenience sampling method. Fifty-one student nurses from August 2019 to January 2021 were designated as the control group, and 51 student nurses from February 2021 to July 2022 were included as the intervention group. The control group received traditional teaching methods, while the intervention group was trained based on TfU framework. The impact was gauged through medical education environment perception, theory and practice assessments, and learning satisfaction surveys. Results After the training, there was no significant difference between the control group and the intervention group in the theory assessment. However, the practice assessment scores of the intervention group were significantly higher than those of the control group. Compared with the control group, the learning satisfaction scores of the trained nurses in the intervention group were significantly higher, exhibiting significant differences, particularly in communication ability, teamwork cooperation, summing up capability, and interest in learning improvement. Furthermore, the scores of the learning perceptions, atmosphere, social self-perceptions, and total scores of the intervention group were significantly higher. Conclusion Training using TfU framework can heighten students’ understanding and command over knowledge and skills, fuel their learning fervor, and enhance their communication and collaboration abilities. TfU framework should be disseminated in medical education to improve the quality of education.
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- 2024
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35. Spatial and Temporal Diversity of Fishes at Wuzhizhou Island in the South China Sea Based on Environmental DNA
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Ting Jiang, Kun Cao, Xin Wang, Rui Xi, Chongzhao Wang, Rui Yang, Wei Yang, Zhenhua Ma, and Yingchun Xing
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α-diversity ,β-diversity ,coral reef fishes ,tropical ocean ,eDNA ,Biology (General) ,QH301-705.5 - Abstract
Wuzhizhou Island (WZZ) is one of the most mature tourism islands on the coast of the South China Sea, and its surrounding waters are rich in tropical coral reef fishes. Tourism could affect marine biodiversity, especially the coral reef fish community. In order to understand the species diversity of fish surrounding WZZ, environmental DNA (eDNA) samples were collected from six sampling sites around the island, located in the core and non-core tourism areas, respectively, from 2022 to 2023. In total, 188 fish species, belonging to 124 genera, 63 families and 17 orders, were detected using eDNA method. The Perciformes contained the highest number of species (n = 130, 69.15% of total number). Compared to traditional fishing nets, eDNA could provide more information on fish species. The results indicated that species richness and Margalef’s index were higher at the sampling sites in the core area of tourism of WZZ, which maybe relevant to tourism-based provisioning. By contrast, the Shannon–Wiener index, Pielou’s index and dominant species of fish did not display significant differences between core and non-core tourism areas. On the basis of the results of the β-diversity index, the differences in fish communities had a closer relationship to geographical location. Furthermore, there were obvious differences in fish communities in different seasons, which may be attributed to the influence of ocean currents.
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- 2024
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36. Effect of Dietary Concentrate-to-Forage Ratios During the Cold Season on Slaughter Performance, Meat Quality, Rumen Fermentation and Gut Microbiota of Tibetan Sheep
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Shijia Wang, Wenhui Tang, Ting Jiang, Ru Wang, Ruoxi Zhang, Jingyu Ou, Qiangjun Wang, Xiao Cheng, Chunhuan Ren, Jiahong Chen, Yafeng Huang, and Zijun Zhang
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Tibetan sheep ,carcass traits ,meat quality ,gut microbiota ,correlation analysis ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
This study aimed to investigate the effects of different dietary concentrate-to-forage ratios on slaughter performance, meat quality, rumen fermentation, rumen microbiota and fecal microbiota in Tibetan sheep. A total of sixty male Tibetan sheep were equally allocated into three dietary groups based on concentrate-to-forage ratios, i.e., 30:70 (C30), 50:50 (C50), and 70:30 (C70). Compared with the C30 group, sheep fed the C70 diet resulted in a higher (p < 0.05) slaughter live weight (SLW), hot carcass weight (HCW), dressing percentage (DP), eye muscle area, average daily gain (ADG), and ruminal total volatile fatty acids concentration and propionate molar proportion and lower (p < 0.05) shear force and cooking loss of meat, and ruminal acetate molar proportion and acetate:propionate ratio. Sheep in the C50 group exhibited a higher (p < 0.05) SLW, HCW, ADG, and ruminal propionate molar proportion and lower (p < 0.05) shear force and cooking loss of meat, and ruminal acetate molar proportion and acetate: propionate ratio compared with the C30 group. In rumen fluid, the relative abundance of Butyrivibrio was lower (p = 0.031) in the C30 group, and that of Ruminococcus was higher (p = 0.003) in the C70 group compared with the C50 group. In feces, genus Monoglobus and UCG_002 were the most abundant in the C30 group (p < 0.05), and the relative abundance of Prevotella was significantly higher in the C70 group than in other groups (p = 0.013). Correlation analysis revealed possible links between slaughter performance and meat quality and altered microbiota composition in the rumen and feces of Tibetan sheep. Overall, feeding a C70 diet resulted in superior carcass characteristics and meat quality in Tibetan sheep, thus laying a theoretical basis for the application of short-term remote feeding during the cold season.
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- 2024
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37. Refractory pruritus caused by sintilimab and its clinical management: A case report
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Li Chen, Xin Cao, Xing Luo, and Ting Jiang
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Case report ,Sintilimab ,Naloxone ,Refractory pruritus ,Immune checkpoint inhibitors ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Several immune related adverse events (irAEs) were reported with the wide application of immune checkpoint inhibitors (ICIs) in tumors. ICI-related skin reactions are the most common, which are manifested as maculopapules, rash, pruritus, vitiligo, psoriasis, and lichenoid rash.Among them, the incidence of pruritus is second only to maculopapule/rash, but both often co-exist. The severity of pruritus is mostly mild to moderate and can be relieved after symptomatic treatment with antihistamines. Symptoms are slightly relieved after conventional treatment in patients with severe pruritus, but it easily recurs and eventually develops into refractory pruritus.The patient's quality of life may be affected and may also be life-threatening.We report a case of a patient with postoperative recurrence of gallbladder neuroendocrine carcinoma,who developed refractory pruritus after sintilimab use, which was relieved after naloxone infusion after unsuccessful conventional drug therapy. By analyzing the treatment plan of this typical case of immune-related refractory pruritus after using sintilimab, this report discusses how clinical pharmacists can provide individualized treatment of patients by using their expertise and clinicians' cooperation and complementation in treating clinically difficult cases. This case report may be used as a reference in treating patients with refractory pruritus after the clinical use of sintilimab.
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- 2024
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38. Identifying therapeutic targets for breast cancer: insights from systematic Mendelian randomization analysis
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Tao Yao, Yun-Lu Lin, Yu-Qing Wu, Xin-Ge Qian, Zhe-Ning Wang, Sang Qian, Ting Jiang, Jing-Chen Liu, Luo-Xiang Fang, Cheng Zhen, and Chun-Hui Wu
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breast cancer ,drug targets ,Mendelian randomization ,single-nucleotide polymorphism ,genetic approaches ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
BackgroundBreast cancer (BC) exhibits a high incidence rate, imposing a substantial burden on healthcare systems. Novel drug targets are urgently needed for BC. Mendelian randomization (MR) has gained widespread application for identifying fresh therapeutic targets. Our endeavor was to pinpoint circulatory proteins causally linked to BC risk and proffer potential treatment targets for BC.MethodsThrough amalgamating protein quantitative trait loci from 2,004 circulating proteins and comprehensive genome-wide association study data from the Breast Cancer Association Consortium, we conducted MR analyses. Employing Steiger filtering, bidirectional MR, Bayesian colocalization, phenotype scanning, and replication analyses, we further solidified MR study outcomes. Additionally, protein-protein interaction (PPI) network was harnessed to unveil latent associations between proteins and prevailing breast cancer medications. The phenome-wide MR (Phe-MR) was employed to assess potential side effects and indications for the druggable proteins of BC. Finally, we further affirmed the drugability of potential drug targets through mRNA expression analysis and molecular docking.ResultsThrough comprehensive analysis, we identified five potential drug targets, comprising four (TLR1, A4GALT, SNUPN, and CTSF) for BC and one (TLR1) for BC_estrogen receptor positive. None of these five potential drug targets displayed reverse causation. Bayesian colocalization suggested that these five latent drug targets shared variability with breast cancer. All drug targets were replicated within the deCODE cohort. TLR1 exhibited PPI with current breast cancer therapeutic targets. Furthermore, Phe-MR unveiled certain adverse effects solely for TLR1 and SNUPN.ConclusionOur study uncovers five prospective drug targets for BC and its subtypes, warranting further clinical exploration.
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- 2024
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39. Immune cells mediated the causal relationship between the gut microbiota and lung cancer: a Mendelian randomization study
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Zhiting Chen, Zhe Wang, Hao Ma, Hejing Bao, Ting Jiang, Ting Yang, and Shudong Ma
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lung cancer ,gut microbiota ,immune cell ,gut-lung axis ,Mendelian randomization ,Microbiology ,QR1-502 - Abstract
IntroductionThe gut microbiota (GM) influences the occurrence and progression of lung cancer (LC), with potential involvement of immune cells (IC). We aimed to investigate the causal impact of GM on LC and identify potential immune cell mediators.MethodsThe utilized data for the Genome-Wide Association Studies (GWAS) were summarized as follows: gut microbiota data from the Dutch Microbiome Project (DMP) (N = 7,738), lung cancer data from the Transdisciplinary Research in Cancer of the Lung (TRICL) and International Lung Cancer Consortium (ILCCO) (Ncase = 29,266, Ncontrol = 56,450) included four types of cancer: NSCLC, LUAD, LUSC, and SCLC, and immune cell data from European populations (N = 3,757). We employed bi-directional two-sample univariable Mendelian randomization (UVMR), multivariable Mendelian randomization (MVMR), and mediation analysis to assess the causal relationship between GM and LC and potential immune cell mediators.ResultsBi-directional UVMR analysis revealed that 24 gut microbiota species can affect LC, while LC can affect the abundance of 17 gut microbiota species. Mediation analysis demonstrated that six immune cells mediated the causal relationships of seven gut microbiota species on LC: “CCR7 on naive CD8+ T cell” mediated the causal relationship between s_Alistipes_putredinis and LUAD, with a mediation proportion of 9.5% and P = 0.018; “IgD− CD27− B cell %lymphocyte” mediated the causal relationships between g_Gordonibacter and s_Gordonibacter_pamelaeae with LUSC, with mediation proportions of 11.8% and 11.9%, respectively and P = 0.029; “CD20− CD38− B cell %lymphocyte” mediated the causal relationship between s_Bacteroides_clarus and SCLC, with a mediation proportion of 13.8% and P = 0.005; “CD20 on IgD+ CD38− unswitched memory B cell” mediated the causal relationship between s_Streptococcus_thermophilus and SCLC, with a mediation proportion of 14.1% and P = 0.023; “HLA DR on CD14− CD16+ monocyte” mediated the causal relationship between s_Bifidobacterium_bifidum and SCLC, with a mediation proportion of 8.7% and P = 0.012; “CD45 on Granulocytic Myeloid-Derived Suppressor Cells” mediated the causal relationship between f_Lactobacillaceae and SCLC, with a mediation proportion of 4.0% and P = 0.021.ConclusionThis Mendelian randomization study identified several specific gut microbiotas that exhibit causal relationships with lung cancer and potentially mediate immune cells.
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- 2024
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40. Theoretical proposal for actively manipulating optical differential operation in the quasi-PT symmetric structure
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Zhaoxin Wen, Zhuolang Liao, Ting Jiang, Yifei Song, Yiqing Lu, and Zhaoming Luo
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Optical differential operation ,Photonic spin Hall effect ,Graphene ,Quasi-exceptional point ,Physics ,QC1-999 - Abstract
Optical differential operation is an important scheme for image edge detection due to its advantages of high efficiency, real time and low consumption. In this paper, the actively manipulating optical differential operation is proposed in a quasi-PT-symmetric structure containing graphene, and applied to realize edge imaging with tunable contrast. It is found that there are abrupt changes of the in-plane shift and transverse shift in the photonic spin Hall effect at the Fermi energy of 0.4 eV, where the quasi-exceptional points exist according to the degeneracy analysis of scattering matrix eigenvalues. Based on the photonic spin Hall effect near the quasi-exceptional points, we design the optical differential operation whose spatial transfer function is actively manipulated by changing the external electric field to adjust the Fermi energy of graphene. Finally, the edge imaging with different contrast can also be achieved when the optical differential operation is applied to image processing. The actively manipulating optical differential operation opens up new possibilities in microscopic imaging and smart driving.
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- 2024
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41. Activation of TLR9 signaling suppresses the immunomodulating functions of CD55lo fibroblastic reticular cells during bacterial peritonitis
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Ting Jiang, Yiming Li, Xingping Huang, Preethi Jayakumar, Timothy R. Billiar, and Meihong Deng
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fibroblastic reticular cells ,fat-associated lymphoid cluster ,TLR9 ,peritoneal immunity ,peritonitis ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Fibroblastic reticular cells (FRCs) are a subpopulation of stromal cells modulating the immune environments in health and disease. We have previously shown that activation of TLR9 signaling in FRC in fat-associated lymphoid clusters (FALC) regulate peritoneal immunity via suppressing immune cell recruitment and peritoneal resident macrophage (PRM) retention. However, FRCs are heterogeneous across tissues and organs. The functions of each FRC subset and the regulation of TLR9 in distinct FRC subsets are unknown. Here, we confirmed that specific deletion of TLR9 in FRC improved bacterial clearance and survival during peritoneal infection. Furthermore, using single-cell RNA sequencing, we found two subsets of FRCs (CD55hi and CD55lo) in the mesenteric FALC. The CD55hi FRCs were enriched in gene expression related to extracellular matrix formation. The CD55lo FRCs were enriched in gene expression related to immune response. Interestingly, we found that TLR9 is dominantly expressed in the CD55lo subset. Activation of TLR9 signaling suppressed proliferation, cytokine production, and retinoid metabolism in the CD55lo FRC, but not CD55hi FRC. Notably, we found that adoptive transfer of Tlr9-/–CD55lo FRC from mesenteric FALC more effectively improved the survival during peritonitis compared with WT-FRC or Tlr9-/–CD55hi FRC. Furthermore, we identified CD55hi and CD55lo subsets in human adipose tissue-derived FRC and confirmed the suppressive effect of TLR9 on the proliferation and cytokine production in the CD55lo subset. Therefore, inhibition of TLR9 in the CD55lo FRCs from adipose tissue could be a useful strategy to improve the therapeutic efficacy of FRC-based therapy for peritonitis.
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- 2024
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42. NMR-based metabolomic analysis of plasma from elderly patients with CVD before and after using contrast media
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Qian Du, Ting Jiang, Qiuju Yuan, Yuanyuan Bai, Donghai Lin, and Donghui Liu
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NMR metabolomics ,CVD ,Contrast media ,Plasma ,Biomarkers ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Contrast-induced acute kidney injury (CI-AKI) is a growingly common kidney problem caused by medical procedures involving contrast media (CM), especially in older patients with existing health issues. It is crucial to pinpoint potential biomarkers for the early detection of CI-AKI. Previously, we observed that iodixanol affects glucose, choline, and glutathione metabolism in endothelial cells under laboratory conditions. In this study, we used 1H NMR-based metabolomics to examine the metabolic changes in the blood plasma of elderly patients with cardiovascular disease (CVD) before and after receiving iodixanol. We identified altered metabolites in plasma 24 and 48 h after iodixanol injection compared to levels before injection. Notably, metabolites such as glucose, unsaturated fatty acids (UFA), low-density lipoprotein (LDL)/very low-density lipoprotein (VLDL), pyruvate, choline, and glycine showed potential as biomarkers at 24 h post-injection compared to levels before injection. Similarly, glucose, pyruvate, lactate, choline, and glycine in plasma could serve as potential biomarkers at 48 h post-injection. Iodixanol notably affected pathways related to glycolysis, fatty acid breakdown, and amino acid metabolism according to our metabolic pathway analysis. The altered levels of specific metabolites in plasma could be indicative of CM-induced kidney injury. Overall, this research aids in understanding the physiological mechanisms involved and in identifying early biomarkers and prevention strategies for CI-AKI.
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- 2024
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43. Understanding and comparing risk factors and subtypes in South Korean adult and adolescent women's suicidal ideation or suicide attempt using survey and social media data
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Donghun Kim, Ting Jiang, Ji Hyun Baek, Sou Hyun Jang, and Yongjun Zhu
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Computer applications to medicine. Medical informatics ,R858-859.7 - Abstract
Objective This study aimed to investigate the similarities and differences in risk factors for suicide among adult and adolescent women in South Korea and identify subtypes of suicidal ideation or suicide attempt in each group. Methods Multifaceted data were collected and analyzed by linking survey and social media data. Interpretable machine learning models were constructed to predict suicide risk and major risk factors were extracted by investigating their feature importance. Additionally, subtypes of suicidal adult and adolescent women were identified and explained using risk factors. Results The risk factors for adult women were primarily related to mental disorders, while those for adolescent women were primarily related to interpersonal experiences and needs. Two subtypes of suicidal adult women were one with high psychiatric symptoms and mental disorders of them and/or their families and the other with excessive social media use and high online victimization. Two subtypes of suicidal adolescent women were one with high psychiatric symptoms, high ACEs, and high social connectedness, and the other with frequent social media use, high online sexual victimization, and high social assurance. Conclusions These findings enable a stratified and targeted understanding of suicide in women and help develop customized suicide prevention plans in South Korea.
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- 2024
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44. Up-to-date quality survey and evaluation of neonatal screening programs in China
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Jinming Zhang, Lizi Jin, Penghui Feng, Yu Fei, Wen Li, Ting Jiang, Zehao Zhang, and Falin He
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Neonatal screening ,Quality management ,Quality performance ,Quality evaluation ,Pediatrics ,RJ1-570 - Abstract
Abstract Aims To thoroughly evaluate the quality of the entire process of neonatal screening (NBS) in China. Methods We collected survey questionnaires from 54.4% (135/248) of NBS institutions in China and conducted on-site visits to 20 of these facilities to validate the data. The quality performance of the institutions was evaluated, and differences across various factors were analysed. Results Merely 62.5% of the provinces had acceptable performance in neonatal screening. Institutions with limited staff were more prone to organizational management shortcomings. Institutions in provinces with a per capita GDP below 10,000 USD exhibited lower quality control levels than those with a per capita GDP between 10,000 and 15,000 USD. Obstetrics departments have a lower awareness of quality control compared to other blood collection facilities. Conclusions A nationwide, comprehensive quality control system for continuous enhancements in quality management, screening, diagnosis, and treatment is imperative to ensure prompt diagnosis and intervention.
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- 2024
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45. High stress zone detection technology of coal face based on seismic slot wave and P-wave
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Xiaoshuai YAO, Mingfang JIN, Ting JIANG, and Wanpeng ZHANG
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rock burst ,high stress ,uniaxial compression ,velocity of channel wave ,velocity of p-wave ,positive correlation ,Mining engineering. Metallurgy ,TN1-997 - Abstract
As the coal mining area shifts towards deeper depths, mining depth continues to increase, mining conditions become more complex, and ground pressure disasters become increasingly severe. There is an urgent need for accurate detection methods capable of spatially detecting high-stress areas in coal mining faces. Through uniaxial compression test, the variation of seismic longitudinal wave velocity of coal and rock samples under uniaxial compression was studied. It was found that during the elastic stage of uniaxial compression, the axial seismic longitudinal wave velocity of coal and rock samples increased with increasing stress until the coal and rock blocks were damaged, and the wave velocity rapidly decreased. The response curve of seismic wave velocity to stress showed that there was a positive correlation between seismic longitudinal wave and stress during the elastic stage of coal and rock samples; at the same time, the response curve characteristics of seismic slot wave velocity and stress under uniaxial compression conditions were also studied, and it was found that there was a positive correlation between seismic slot wave and stress, which could also be used for exploring high-stress areas in coal mining working face. It is proposed to use seismic slot wave and longitudinal wave together to detect stress concentration areas. Simple geological conditions of coal mining working face were selected for high-stress detection experiments, and the initial arrival speed of longitudinal wave and the speed at a specific frequency of slot wave were extracted from the test data. After CT tomography imaging, a contour map of wave velocity was formed. Then, combined with the relevant information of roadway, the high-speed area was circled respectively, and according to the positive correlation between seismic wave velocity and stress, the high-speed area was interpreted as a high-stress area. Finally, the overlapping area of the two was taken as a high-risk area of impact ground pressure in the working face. Through drilling and mining verification: when drilling in the stress overlap area, the amount of coal powder was obviously larger, easy to shrink holes; when mining passed through the stress overlap area, the deformation of roadway was relatively large, and “coal cannon” occasionally occurred. The joint exploration test shows that trough waves can be used to detect high-stress areas in coal mining working face, and combining longitudinal waves can overcome the limitations of single geophysical exploration means and more accurately detect high-stress areas in impact ground pressure mines.
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- 2023
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46. Retina-inspired organic neuromorphic vision sensor with polarity modulation for decoding light information
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Ting Jiang, Yiru Wang, Wanxin Huang, Haifeng Ling, Guofeng Tian, Yunfeng Deng, Yanhou Geng, Deyang Ji, and Wenping Hu
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Applied optics. Photonics ,TA1501-1820 ,Optics. Light ,QC350-467 - Abstract
Abstract The neuromorphic vision sensor (NeuVS), which is based on organic field-effect transistors (OFETs), uses polar functional groups (PFGs) in polymer dielectrics as interfacial units to control charge carriers. However, the mechanism of modulating charge transport on basis of PFGs in devices is unclear. Here, the carboxyl group is introduced into polymer dielectrics in this study, and it can induce the charge transfer process at the semiconductor/dielectric interfaces for effective carrier transport, giving rise to the best device mobility up to 20 cm2 V−1 s−1 at a low operating voltage of −1 V. Furthermore, the polarity modulation effect could further increase the optical figures of merit in NeuVS devices by at least an order of magnitude more than the devices using carboxyl group-free polymer dielectrics. Additionally, devices containing carboxyl groups improved image sensing for light information decoding with 52 grayscale signals and memory capabilities at an incredibly low power consumption of 1.25 fJ/spike. Our findings provide insight into the production of high-performance polymer dielectrics for NeuVS devices.
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- 2023
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47. Triplet‐drug chemotherapy combined with anti‐EGFR antibody as an effective therapy for patients with initially unresectable metastatic colorectal cancer: a meta-analysis
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Muyou Tian, Huifen Li, Wenjing Dong, Yuhong Li, Ting Jiang, Yanhua Lv, Jianxiong Zeng, Xiaomei Jiang, Zhaofeng Yin, and Jianjun Xiao
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Unresectable metastatic colorectal cancer, Triplet ,Drug chemotherapy, Anti ,EGFR antibody ,Surgery ,RD1-811 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract The meta-analysis aimed to assess the clinical efficacy of chemotherapeutic triplet‐drug regimen combined with anti‐EGFR antibody in patients with initially unresectable metastatic colorectal cancer (mCRC). A systematic literature search was performed in PubMed Publisher. Studies evaluating FOLFOXIRI combine with panitumumab or cetuximab as the therapy for initially unresectable mCRC were included. The primary outcome was objective response rate (ORR) and rate of R0 resections. The secondary outcomes included overall survival (OS), progression-free survival (PFS), and grades 3 or 4 adverse events. R software (version 4.0.2) and RevMan (version 5.3) were used to analyze the extracted data. The studies included were published between 2010 and 2021, involving four single-arm phase II trials and two randomized phase II trials. A total of 6 studies with 282 patients were included. The data showed a significant benefit for the FOLFOXIRI + anti-EGFR antibody arm compared with FOLFOXIRI arm (RR 1.33; 95% CI, 1.13–1.58; I 2 = 0%, P
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- 2023
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48. Hay Yield, Chemical Composition, and In Vitro Digestibility of Five Varieties of Common Vetch
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Xiao Cheng, Sunze Wang, Kefan Zhang, Ting Jiang, Yang Ye, Yuan Lu, Yajie Yu, Huiqing Wei, Zijun Zhang, and Yafeng Huang
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varieties ,common vetch ,principal component analysis ,high-quality forage ,Agriculture (General) ,S1-972 - Abstract
Diversifying feed with annual legumes could help to reduce reliance on imported sources of alfalfa hay, ensuring a consistent supply of forage throughout year, and potentially decreasing the cost of raising ruminants. This study evaluated the varietal variability in hay yield, chemical composition, carbohydrate and protein fractions, and in vitro digestibility of five common vetch varieties used in this experiment during the full-flowering period in Jianghuai region during the 2022 crop season. Results showed that improved varieties (Lanjian No. 1 and Lanjian No. 2, 6.30 and 6.11 t DM/ha) had significantly higher hay yields than the local variety (333A, Longjian No. 1), with Lanjian No. 3 showing intermediate yields. Variety Lanjian No. 1 had the highest non-protein nitrogen concentration and in vitro digestibility, while Lanjian No. 2 had the highest starch concentration, fraction of intermediately degradable pectin and starch, and fraction of neutral detergent-soluble protein and acid detergent-soluble protein. Pearson correlation showed that hay yield was not significantly correlated with quality parameters. Principal component analysis showed that Lanjian No. 1 and Lanjian No. 2 received higher nutritive value. In summary, Lanjian No. 1 and Lanjian No. 2 had better potential use as high-protein feedstuffs for dairy cattle and sheep in the Jianghuai region based on yield, protein fractions, and in vitro digestibility.
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- 2024
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49. Preparation and Antigenic Site Identification of Monoclonal Antibodies against PB1 Protein of H9N2 Subtype AIV
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Yiqin Cai, Guihu Yin, Jianing Hu, Ye Liu, Xiangyu Huang, Zichen Gao, Xinyu Guo, Ting Jiang, Haifeng Sun, and Xiuli Feng
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H9N2 subtype AIV ,PB1 protein ,monoclonal antibody ,antigenic site ,Veterinary medicine ,SF600-1100 - Abstract
Recently, low pathogenic avian influenza virus (LPAIV), including H9N2 subtype, has been common clinical epidemic strains, and is widely distributed globally. The PB1 protein is a key component of the viral RNA polymerase complex (vRNP), and is vital to viral transcription and translation. In this study, to investigate the antigenic determinants in the PB1 protein, the truncated PB1 sequence (1bp-735bp) from H9N2 subtype AIV was amplified with PCR, and expressed in plasmid pET-28a (+). After purification, the recombinant PB1 protein was used to immunize BALB/c mice. Following immunization, hybridoma cells producing PB1-specific monoclonal antibodies were generated through the fusion of splenic lymphocytes with SP2/0 cells. Then, four stable hybridoma cell lines (5F12, 5B3, 2H9, and 3E6) were screened using indirect ELISA and Western blotting. Furthermore, two antigenic sites, 67NPIDGPLPED76 and 97ESHPGIFENS106, were identified through the construction of truncated overlapping fragments of the PB1 protein. These sites were conserved among 28 AIV strains, and were located on the PB1 protein surface. The findings offer a theoretical reference for the development and improvement of H9N2 vaccines and offer biological materials for virus detection during AIV infection mechanisms.
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- 2024
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50. Identification of the mitochondrial protein POLRMT as a potential therapeutic target of prostate cancer
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Xiaojun Li, Linya Yao, Tao Wang, Xiaolei Gu, Yufan Wu, and Ting Jiang
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Cytology ,QH573-671 - Abstract
Abstract RNA polymerase mitochondria (POLRMT) is essential for mitochondrial transcription machinery and other mitochondrial functions. Its expression and potential functions in prostate cancer were explored here. The Cancer Genome Atlas prostate cancer cohort (TCGA PRAD) shows that POLRMT mRNA expression is upregulated in prostate cancer tissues and POLRMT upregulation is correlated with poor patients’ survival. POLRMT mRNA and protein levels were upregulated in local prostate cancer tissues and different primary/immortalized prostate cancer cells. Genetic depletion of POLRMT, using viral shRNA or CRISPR/Cas9 gene editing methods, impaired mitochondrial functions in prostate cancer cells, leading to mitochondrial depolarization, oxidative stress, mitochondria complex I inhibition, and ATP depletion. Moreover, POLRMT depletion resulted in robust inhibition of prostate cancer cell viability, proliferation, and migration, and provoked apoptosis. Conversely, prostate cancer cell proliferation, migration, and ATP contents were strengthened following ectopic POLRMT overexpression. In vivo, intratumoral injection of POLRMT shRNA adeno-associated virus impeded prostate cancer xenograft growth in nude mice. POLRMT silencing, oxidative stress, and ATP depletion were detected in POLRMT shRNA-treated prostate cancer xenograft tissues. IMT1 (inhibitor of mitochondrial transcription 1), the first-in-class POLRMT inhibitor, inhibited prostate cancer cell growth in vitro and in vivo. Together, overexpressed POLRMT is an important mitochondrial protein for prostate cancer cell growth, representing a novel and promising diagnostic and therapeutic oncotarget.
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- 2023
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