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12 results on '"Tinh, Nguyen Thi Ngoc"'

7. Selection of Lactic Acid Bacteria (LAB) Antagonizing

Author

Linh Nguyen Thi, Truc, Ai Trinh, Ngoc, To Tran Thi, Hong, Tuu Nguyen, Thanh, Huong Huynh, Kim, Long Pham, Kim, Giang Huynh, Truong, Phu Truong, Quoc, and Tinh Nguyen Thi, Ngoc

Subjects
LAB, AHPND, fungi, whiteleg shrimp, Vibrio parahaemolyticus, Article
Abstract

Acute hepatopancreatic necrosis disease (AHPND) has recently emerged as a serious disease of cultured shrimp. A total of 19 lactic acid bacteria (LAB) strains isolated from shrimp samples were characterized based on morphological characteristics, biochemical tests, sequencing analysis, and their ability to antagonize Vibrio parahaemolyticus, which causes AHPND in whiteleg shrimp. Results from the agar well diffusion method indicated that 3 out of 19 isolated LAB strains showed the highest antagonizing ability against AHPND V. parahaemolyticus strain with an inhibition zone diameter ranging from 18 to 20 mm. Experiments where shrimps were given feed supplemented with these LAB strains and challenged with AHPND strain showed high survival rates (approximately 80.0%), which were not significantly different as compared to those recorded in the negative control treatment (86.6%), but significantly different to those recorded in the positive control treatment (40.6%) after 16 days of the experiment. However, the histological images of shrimp hepatopancreas indicated that the infection rate significantly reduced from 60.0% to 11.1% in shrimps fed with LAB-supplemented feeds and challenged with AHPND V. parahaemolyticus strain as compared to those in the positive control treatment. A polymerase chain reaction (PCR) and 16S rRNA gene sequencing confirmed the identification of LAB strain. These results can be applied in further experiments to investigate the ability of L. plantarum in preventing AHPND in intensively cultured whiteleg shrimp.

Published
2019

9. Selection of Lactic Acid Bacteria (LAB) Antagonizing Vibrio parahaemolyticus: The Pathogen of Acute Hepatopancreatic Necrosis Disease (AHPND) in Whiteleg Shrimp (Penaeus vannamei)

Author

Ai Trinh Ngoc, To Tran Thi Hong, Huong Huynh Kim, Linh Nguyen Thi Truc, Giang Huynh Truong, Phu Truong Quoc, Tinh Nguyen Thi Ngoc, Long Pham Kim, and Tuu Nguyen Thanh

Subjects
0301 basic medicine, whiteleg shrimp, Biology, General Biochemistry, Genetics and Molecular Biology, Microbiology, law.invention, 03 medical and health sciences, law, Whiteleg shrimp, Penaeus, Pathogen, Polymerase chain reaction, LAB, General Immunology and Microbiology, Vibrio parahaemolyticus, fungi, 04 agricultural and veterinary sciences, biology.organism_classification, Shrimp, AHPND, 030104 developmental biology, 040102 fisheries, 0401 agriculture, forestry, and fisheries, Hepatopancreas, General Agricultural and Biological Sciences, Bacteria
Abstract

Acute hepatopancreatic necrosis disease (AHPND) has recently emerged as a serious disease of cultured shrimp. A total of 19 lactic acid bacteria (LAB) strains isolated from shrimp samples were characterized based on morphological characteristics, biochemical tests, sequencing analysis, and their ability to antagonize Vibrio parahaemolyticus, which causes AHPND in whiteleg shrimp. Results from the agar well diffusion method indicated that 3 out of 19 isolated LAB strains showed the highest antagonizing ability against AHPND V. parahaemolyticus strain with an inhibition zone diameter ranging from 18 to 20 mm. Experiments where shrimps were given feed supplemented with these LAB strains and challenged with AHPND strain showed high survival rates (approximately 80.0%), which were not significantly different as compared to those recorded in the negative control treatment (86.6%), but significantly different to those recorded in the positive control treatment (40.6%) after 16 days of the experiment. However, the histological images of shrimp hepatopancreas indicated that the infection rate significantly reduced from 60.0% to 11.1% in shrimps fed with LAB-supplemented feeds and challenged with AHPND V. parahaemolyticus strain as compared to those in the positive control treatment. A polymerase chain reaction (PCR) and 16S rRNA gene sequencing confirmed the identification of LAB strain. These results can be applied in further experiments to investigate the ability of L. plantarum in preventing AHPND in intensively cultured whiteleg shrimp.

Published
2019

12. Gnotobiotically grown rotifer Brachionus plicatilis sensu strictu as a tool for evaluation of microbial functions and nutritional value of different food types

Author

Tinh, Nguyen Thi Ngoc, Nguyen Ngoc Phuoc, Dierckens, Kristof, Sorgeloos, Patrick, and Bossier, Peter

Subjects
*LEAVENING agents, *YEAST, *EGGS, *SULFATES
Abstract

Abstract: Axenic rotifers (Brachionus plicatilis sensu strictu, clone 10) were obtained by treating amictic eggs with glutaraldehyde. Depending of the batch of rotifers, total disinfection could be obtained by exposure to 50–100 ppm from 1 to 2 h at 28 °C. The hatched axenic neonates were used to test the effect of microbial communities (MCs) which were isolated from either normal-performing or crashed rotifer cultures. These MCs were either used directly or were first regrown on Marine Agar. MCs were introduced to gnotobiotic Brachionus cultures in combination with three different food types, i.e. Chlorella, wild-type yeast and the mnn9 yeast mutant, which is deficient in cell wall-bound mannoprotein. In the absence of MCs or when heat-killed MCs were added, Chlorella was always the best food, while lower growth rates were observed with wild-type yeast and the mnn9 mutant as food. In the presence of live MCs and when rotifers were fed with Chlorella, the added MCs had no effect on rotifer performance. When yeasts were used as major food, all the tested MCs were able to increase the rotifer growth rate. The experiments with heat-killed MCs yielded no increase in rotifer growth rate, suggesting that the observed enhancement in rotifer growth rate was truly a probiotic effect rather than a nutritional effect. The results of this study demonstrate that gnotobiotic rotifer cultures obtained from axenic amictic eggs can be used as a test system for studying microbial-attributed as well as nutritional functions in the aquatic food chain. In addition, since the MCs originating from the crashed rotifer cultures did not decrease the growth rate in the tested rotifer cultures, it is likely that the observed crashes were not due to the presence of a standing deleterious MC. [Copyright &y& Elsevier]

Published
2006
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