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Background The most ordinary subtype of lung cancer is lung adenocarcinoma (LuAC), which is characterized by strong metastatic ability. And LuAC rates in Xuanwei leads to the poor prognosis and high death rate. In this study, we systematically explored the molecular mechanism of LuAC bone metastasis in Xuanwei by transcriptome sequencing. Methods RNA Sequencing was conducted to explore the noncoding RNAs (ncRNAs) expression profiles in primary LuAC and LuAC bone metastasis. We identified differentially expressed mRNAs (DEmRNAs), miRNAs (DEmiRNAs), lncRNAs (DElncRNAs) and circRNAs (DEcircRNAs). Bioinformatics analyses the possible relationships and functions of the LuAC bone metastasis-related competing endogenous RNA (ceRNA). And qRT-PCR was performed to evaluate the expression of these differently expressed genes in serum. Results A total of 2,141 DEmRNAs, 43 DEmiRNAs, 136 DElncRNAs and 706 DEcircRNAs were identified in the Xuanwei patients with primary LuAC vs. LuAC bone metastasis, respectively. The circRNA/miRNA/mRNA and lncRNA/miRNA/mRNA networks of LuAC in Xuanwei with bone metastasis were built, and the gene expression mechanisms regulated by ncRNAs were unveiled via the ceRNA regulatory networks. We observe that lncRNA (ADAMTS9-AS2, TEX41, DLEU2, LINC00152)-miR-223-3p-SCARB1 and hsa_circ_0000053-miR-196a-5p/miR-196b-5p-HOXA5 ceRNA networks might play an important role in bone metastasis of Xuanwei LuAC. Conclusions We comprehensively identified ceRNA regulatory networks of LuAC in Xuanwei with bone metastasis as well as revealed the contribution of different ncRNAs expression profiles. Our data demonstrate the association between mRNAs and ncRNAs in the metastasis mechanism of LuAC in Xuanwei with bone metastasis.

Distant metastasis is the main cause of death for cancer patients. Recently, the newly discovered programmed cell death includes necroptosis, pyroptosis, and ferroptosis, which possesses an important role in the process of tumor metastasis. At the same time, it is widely reported that non-coding RNA precisely regulates programmed death and tumor metastasis. In the present review, we summarize the function and role of necroptosis, pyrolysis, and ferroptosis involving in cancer metastasis, as well as the regulatory factors, including non-coding RNAs, of necroptosis, pyroptosis, and ferroptosis in the process of tumor metastasis.

Distant metastasis is the main cause of death for cancer patients. Recently, the newly discovered programmed cell death includes necroptosis, pyroptosis, and ferroptosis, which possesses an important role in the process of tumor metastasis. At the same time, it is widely reported that non-coding RNA precisely regulates programmed death and tumor metastasis. In the present review, we summarize the function and role of necroptosis, pyrolysis, and ferroptosis involving in cancer metastasis, as well as the regulatory factors, including non-coding RNAs, of necroptosis, pyroptosis, and ferroptosis in the process of tumor metastasis.

This study aimed to construct a widely accepted prognostic nomogram in Chinese high-grade osteosarcoma (HOS) patients aged ≤ 30 years to provide insight into predicting 5-year overall survival (OS). Data from 503 consecutive HOS patients at our centre between 12/2012 and 05/2019 were retrospectively collected. Eighty-four clinical features and routine laboratory haematological and biochemical testing indicators of each patient at the time of diagnosis were collected. A prognostic nomogram model for predicting OS was constructed based on the Cox proportional hazards model. The performance was assessed by the concordance index (C-index), receiver operating characteristic curve and calibration curve. The utility was evaluated by decision curve analysis. The 5-year OS was 52.1% and 2.6% for the nonmetastatic and metastatic patients, respectively. The nomogram included nine important variables based on a multivariate analysis: tumour stage, surgical type, metastasis, preoperative neoadjuvant chemotherapy cycle, postoperative metastasis time, mean corpuscular volume, tumour-specific growth factor, gamma-glutamyl transferase and creatinine. The calibration curve showed that the nomogram was able to predict 5-year OS accurately. The C-index of the nomogram for OS prediction was 0.795 (range, 0.703–0.887). Moreover, the decision curve analysis curve also demonstrated the clinical benefit of this model. The nomogram provides an individualized risk estimate of the 5-year OS in patients with HOS aged ≤ 30 years in a Chinese population-based cohort.

Background: Ganglion cell paraganglioma is a subtype of neuroendocrine tumors, which is rare in clinical practice. Paraganglioma in the spinal area, especially in the spinal canal, is even rarer.Case presentation:The case we reported was a 46-year-old female who was admitted to the hospital due to "physical examination found a mass in the lumbar spinal canal for more than 2 months." After admission, the relevant examinations and preoperative preparations were perfected, and the posterior L2-3 spinal mass resection + spinal decompression + nail rod system internal fixation was performed. The patient recovered well after the operation, and there was no tumor recurrence in follow-up. Conclusions:Surgical treatment assisted by MRI neuroimaging and 3D printing reconstruction technology may be an effective way to treat paraganglioma in the lumbar spinal canal.

Objective Metastatic epidural spinal cord compression (MESCC) seriously affects the life span and quality of life of patients. Separation surgery combined with vertebroplasty combined with interstitial implantation of 125 I seeds (SSVPI) is a novel treatment for MESCC patients. To investigate the safety, feasibility and clinical efficacy on metastatic epidural spinal cord compression treated by SSVPI. Method Prospective analysis of 56 patients diagnosed with metastatic epidural spinal cord compression (MESCC) and receiving Separation surgery (SS) or separation surgery combined with vertebroplasty combined with interstitial implantation of 125 I seeds. Fifty-six patients were randomly divided into the SS group (26 patients) and SSVPI group (30 patients), and the differences in intraoperative bleeding, postoperative pain visual analog scale scores (VAS), Karnofsky performance scores (KPS) and American Spinal Injury Association grades were compared. Results There was no significant difference in intraoperative bleeding between the SS group and the SSVPI group ( P > 0.05). There was no significant difference at 1 week after surgery ( P > 0.05); there were significant differences in VAS scores at 2, 6 and 12 months after surgery ( P < 0.05). There was no significant difference in the KPS score 1 week after surgery ( P > 0.05); there were statistically significant differences in the KPS scores at 2, 6 and 12 months after surgery ( P < 0.05). In the SS group, there were 20 patients (76.92%) with an improvement in ASIA grades from pre- to postoperation. In the SSVPI group, there were 24 patients (80%) with improved ASIA grades from pre- to postoperation. Conclusion SSVPI is a safe, feasible, less invasive and effective treatment for MESCC patients.

Background: This study purposed to construct a widely accepted prognostic nomogram in Chinese high-grade osteosarcoma (HOS) patients aged ≤ 30 years to provide insight into predicting 5-year overall survival (OS). Methods: Data of 503 consecutive HOS patients at our centre between 12/2012 and 05/2019 were retrospectively collected. The 84 clinical features, routine laboratory haematological and biochemical testing indicators of each patient at the time of diagnosis were collected. A prognostic nomogram model for predicting OS was constructed based on Cox proportional hazards model. The performance was assessed by concordance index (C-index), receiver operating characteristic curve and calibration curve. The utility was evaluated by decision curve analysis. Findings: The 5-year OS was 52.1% and 2.6% for the nonmetastatic and metastatic patients, respectively. The nomogram included nine important variables based on a multivariate analysis: tumour stage, surgical type, metastasis, preoperative neoadjuvant chemotherapy cycle, postoperative metastasis time, mean corpuscular volume, tumour-specific growth factor, gamma-glutamyl transferase and creatinine. The calibration curve showed that the nomogram was able to predict 5-year OS accurately. The C-index of the nomogram for OS prediction was 0.795 (range, 0.703-0.887). Moreover, the decision curve analysis curve also demonstrated the clinical benefit of this model. Interpretation: The nomogram provides an individualized risk estimate of the 5-year OS in patients with HOS aged ≤ 30 years in a Chinese population-based cohort. Funding Statement: This work was financially funded by the National Science Foundation of China (No. U1702283, 81972764, 81960488, 81760520, 81560471), the Joint Special Funds for the Department of Science and Technology of Yunnan Province-Kunming Medical University (No. 2017FE467-073, 2018FE001-060, 2019FE001-152, 2019FE001-078), the Scientific Research Projects of Internal Research Institutions of Medicine (Nos. 2017NS196, 2017NS197, 2018NS069, 2018NS070), the Medical Leaders of Yunnan Province (No. D-201603), the Foundation of the Young and Middle-aged Academic and Technical Leaders of Yunnan Province (No. 2017HB051), China Postdoctoral Science Foundation (No. 2018M643798XB), Training program for 100 Young and middle-aged Academic and technical backbones of Kunming Medical University and the Major Scientific and Technological Achievement Cultivation Project of Kunming Medical University (No. CGPY201703). Declaration of Interests: The authors declare that they have no competing interests. Ethics Approval Statement: All human tissue experiments were approved by the Institutional Ethics Committee of the Third Affiliated Hospital of Kunming Medical University (Yunnan Cancer Hospital). Written informed consent forms for the experimental studies were obtained from the patients or their guardians.

The occurrence of lung cancers is the highest in Xuanwei County, Yunnan province, China, especially among nonsmoking women. Domestic combustion of smoky coal induces serious indoor air pollution and is considered to be the main cause of human lung cancers. The occurrence of lung cancer in Xuanwei County has unique characteristics, such as the high morbidity in nonsmoking women or people with no family history. In the present review, we summarize advances in identification of differentially expressed genes, regulatory lncRNAs and miRNAs in cell proliferation and migration of lung cancers in Xuanwei County. Moreover, several regulatory differentially expressed genes (DEGs) or noncoding RNAs have diagnostic and prognostic significance for lung cancers in Xuanwei County and have the potential to serve as biomarkers.