1. Targeted treatment for biofilm-based infections using PEGylated tobramycin.
- Author
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Ding L, Wang G, Wang J, Peng Y, Cai S, Khan SU, Cui Z, Zhang X, Wu C, and Smyth H
- Subjects
- Animals, Rats, Sprague-Dawley, Male, Rats, Microbial Sensitivity Tests, Tobramycin administration & dosage, Tobramycin pharmacology, Tobramycin therapeutic use, Biofilms drug effects, Polyethylene Glycols chemistry, Pseudomonas aeruginosa drug effects, Anti-Bacterial Agents administration & dosage, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents therapeutic use, Anti-Bacterial Agents chemistry, Pseudomonas Infections drug therapy
- Abstract
Chronic infections often involve biofilm-based bacteria, in which the biofilm results in significant resistance against antimicrobial agents and prevents eradication of the infection. The physicochemical barrier presented by the biofilm matrix is a major impediment to the delivery of many antibiotics. Previously, PEGylation has been shown to improve antibiotic penetration into biofilms in vitro. In these studies, PEGylating tobramycin was investigated both in vitro and in vivo. Two distinct PEGylated tobramycin molecules were synthesized (mPEG-SA-Tob and mPEG-AA-Tob). Then, in a P. aeruginosa biofilm in vitro model, we found that mPEG-SA-Tob can operate as a prodrug and showed 7 times more effectiveness than tobramycin (MIC
80 : 14 μM vs.100 μM). This improved biofilm eradication is attributable to the fact that mPEG-SA-Tob can aid tobramycin to penetrate through the biofilm and overcome the alginate-mediated antibiotic resistance. Finally, we used an in vivo biofilm-based chronic pulmonary infection rat model to confirm the therapeutic impact of mPEG-SA-Tob on biofilm-based chronic lung infection. mPEG-SA-Tob has a better therapeutic impact than tobramycin in that it cannot only stop P. aeruginosa from multiplying in the lungs but can also reduce inflammation caused by infections and prevent a recurrence infection. Overall, our findings show that PEGylated tobramycin is an effective treatment for biofilm-based chronic lung infections., Competing Interests: Declaration of competing interest Hugh D C Smyth, an author of this paper, consults for and has equity ownership in Via Therapeutics, Nob Hill Therapeutics, and Cloxero Therapeutics on inhaled product development. The terms of the arrangement have been reviewed and approved by the University of Texas at Austin following its policy on objectivity in research., (Copyright © 2024. Published by Elsevier B.V.)- Published
- 2024
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