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Your search keyword '"Toledo, Yubisela"' showing total 8 results
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8 results on '"Toledo, Yubisela"'

1. Rationally designed peptide nanosponges for cell-based cancer therapy

Author

Wang, Hongwang, Yapa, Asanka S., Kariyawasam, Nilusha L., Shrestha, Tej B., Kalubowilage, Madumali, Wendel, Sebastian O., Yu, Jing, Pyle, Marla, Basel, Matthew T., Malalasekera, Aruni P., Toledo, Yubisela, Ortega, Raquel, Thapa, Prem S., Huang, Hongzhou, Sun, Susan X., Smith, Paul E., Troyer, Deryl L., and Bossmann, Stefan H.

Published
2017
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2. Peptide nanosponges designed for rapid uptake by leukocytes and neural stem cells

Author

Yapa, Asanka S., Wang, Hongwang, Wendel, Sebastian O., Shrestha, Tej. B., Kariyawasam, Nilusha L., Kalubowilage, Madumali, Perera, Ayomi S., Pyle, Marla, Basel, Matthew T., Malalasekera, Aruni P., Manawadu, Harshi, Yu, Jing, Toledo, Yubisela, Ortega, Raquel, Thapa, Prem S., Smith, Paul E., Troyer, Deryl L., and Bossmann, Stefan H.

Subjects
chemistry
Abstract

The structure of novel binary nanosponges consisting of (cholesterol-(K/D)ₙDEVDGC)₃-trimaleimide units possessing a trigonal maleimide linker, to which either lysine (K)₂₀ or aspartic acid (D)₂₀ are tethered, has been elucidated by means of TEM. A high degree of agreement between these findings and structure predictions through explicit solvent and then coarse-grained molecular dynamics (MD) simulations has been found. Based on the nanosponges' structure and dynamics, caspase-6 mediated release of the model drug 5(6)-carboxyfluorescein has been demonstrated. Furthermore, the binary (DK20) nanosponges have been found to be virtually non-toxic in cultures of neural progenitor cells. It is of a special importance for the future development of cell-based therapies that DK20 nanosponges were taken up efficiently by leucocytes (WBC) in peripheral blood within 3 h of exposure. The percentage of live cells among the WBC was not significantly decreased by the DK20 nanosponges. In contrast to stem cell or leucocyte cell cultures, which have to be matched to the patient, autologous cells are optimal for cell-mediated therapy. Therefore, the nanosponges hold great promise for effective cell-based tumor targeting.

Published
2018

3. Peptide Nanosponges Designed for the Delivery of Perillyl Alcohol to Glioma Cells

Author

Yapa, Asanka S., primary, Shrestha, Tej B., additional, Wendel, Sebastian O., additional, Kalubowilage, Madumali, additional, Yu, Jing, additional, Wang, Hongwang, additional, Pyle, Marla, additional, Basel, Matthew T., additional, Toledo, Yubisela, additional, Ortega, Raquel, additional, Malalasekera, Aruni P., additional, Thapa, Prem S., additional, Troyer, Deryl L., additional, and Bossmann, Stefan H., additional

Published
2018
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4. Early detection of pancreatic cancers in liquid biopsies by ultrasensitive fluorescence nanobiosensors

Author

Kalubowilage, Madumali, primary, Covarrubias-Zambrano, Obdulia, additional, Malalasekera, Aruni P., additional, Wendel, Sebastian O., additional, Wang, Hongwang, additional, Yapa, Asanka S., additional, Chlebanowski, Lauren, additional, Toledo, Yubisela, additional, Ortega, Raquel, additional, Janik, Katharine E., additional, Shrestha, Tej B., additional, Culbertson, Christopher T., additional, Kasi, Anup, additional, Williamson, Stephen, additional, Troyer, Deryl L., additional, and Bossmann, Stefan H., additional

Published
2018
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5. Peptide nanosponges designed for rapid uptake by leukocytes and neural stem cells

Author

Yapa, Asanka S., primary, Wang, Hongwang, additional, Wendel, Sebastian O., additional, Shrestha, Tej. B., additional, Kariyawasam, Nilusha L., additional, Kalubowilage, Madumali, additional, Perera, Ayomi S., additional, Pyle, Marla, additional, Basel, Matthew T., additional, Malalasekera, Aruni P., additional, Manawadu, Harshi, additional, Yu, Jing, additional, Toledo, Yubisela, additional, Ortega, Raquel, additional, Thapa, Prem S., additional, Smith, Paul E., additional, Troyer, Deryl L., additional, and Bossmann, Stefan H., additional

Published
2018
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6. Early breast cancer screening using iron/iron oxide-based nanoplatforms with sub-femtomolar limits of detection

Author

Udukala, Dinusha N, primary, Wang, Hongwang, additional, Wendel, Sebastian O, additional, Malalasekera, Aruni P, additional, Samarakoon, Thilani N, additional, Yapa, Asanka S, additional, Abayaweera, Gayani, additional, Basel, Matthew T, additional, Maynez, Pamela, additional, Ortega, Raquel, additional, Toledo, Yubisela, additional, Bossmann, Leonie, additional, Robinson, Colette, additional, Janik, Katharine E, additional, Koper, Olga B, additional, Li, Ping, additional, Motamedi, Massoud, additional, Higgins, Daniel A, additional, Gadbury, Gary, additional, Zhu, Gaohong, additional, Troyer, Deryl L, additional, and Bossmann, Stefan H, additional

Published
2016
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8. Early detection of pancreatic cancers in liquid biopsies by ultrasensitive fluorescence nanobiosensors.

Author

Kalubowilage M, Covarrubias-Zambrano O, Malalasekera AP, Wendel SO, Wang H, Yapa AS, Chlebanowski L, Toledo Y, Ortega R, Janik KE, Shrestha TB, Culbertson CT, Kasi A, Williamson S, Troyer DL, and Bossmann SH

Subjects
Case-Control Studies, Female, Humans, Liquid Biopsy, Male, Biosensing Techniques, Carcinoma, Pancreatic Ductal diagnosis, Early Detection of Cancer methods, Fluorescent Dyes chemistry, Nanoparticles chemistry, Pancreatic Neoplasms diagnosis
Abstract

Numerous proteases, such as matrix metalloproteinases (MMPs), cathepsins (CTS), and urokinase plasminogen activator (UpA), are dysfunctional (that is, over- or under-expressed) in solid tumors, when compared to healthy human subjects. This offers the opportunity to detect early tumors by liquid biopsies. This approach is of particular advantage for the early detection of pancreatic cancer, which is a "silent killer". We have developed fluorescence nanobiosensors for ultrasensitive (sub-femtomolar) arginase and protease detection, consisting of water-dispersible Fe/Fe 3 O 4 core/shell nanoparticles and two tethered fluorescent dyes: TCPP (Tetrakis(4-carboxyphenyl)porphyrin) and cyanine 5.5. Upon posttranslational modification or enzymatic cleavage, the fluorescence of TCPP increases, which enables the detection of proteases at sub-femtomolar activities utilizing conventional plate readers. We have identified an enzymatic signature for the detection of pancreatic adenocarcinomas in serum, consisting of arginase, matrix metalloproteinase-1, -3, and - 9, cathepsin-B and -E, urokinase plasminogen activator, and neutrophil elastase, which is a potential game-changer., (Copyright © 2018 Elsevier Inc. All rights reserved.)

Published
2018
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