1. Platelet function is modified by common sequence variation in megakaryocyte super enhancers
- Author
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Petersen, Romina, Lambourne, John J., Javierre, Biola M., Grassi, Luigi, Kreuzhuber, Roman, Ruklisa, Dace, Rosa, Isabel M., Tomé, Ana R., Elding, Heather, van Geffen, Johanna P., Jiang, Tao, Farrow, Samantha, Cairns, Jonathan, Al-Subaie, Abeer M., Ashford, Sofie, Attwood, Antony, Batista, Joana, Bouman, Heleen, Burden, Frances, Choudry, Fizzah A., Clarke, Laura, Flicek, Paul, Garner, Stephen F., Haimel, Matthias, Kempster, Carly, Ladopoulos, Vasileios, Lenaerts, An-Sofie, Materek, Paulina M., McKinney, Harriet, Meacham, Stuart, Mead, Daniel, Nagy, Magdolna, Penkett, Christopher J., Rendon, Augusto, Seyres, Denis, Sun, Benjamin, Tuna, Salih, van der Weide, Marie-Elise, Wingett, Steven W., Martens, Joost H., Stegle, Oliver, Richardson, Sylvia, Vallier, Ludovic, Roberts, David J., Freson, Kathleen, Wernisch, Lorenz, Stunnenberg, Hendrik G., Danesh, John, Fraser, Peter, Soranzo, Nicole, Butterworth, Adam S., Heemskerk, Johan W., Turro, Ernest, Spivakov, Mikhail, Ouwehand, Willem H., Astle, William J., Downes, Kate, Kostadima, Myrto, Frontini, Mattia, Lambourne, John [0000-0003-2460-0759], Grassi, Luigi [0000-0002-6308-7540], Haimel, Matthias [0000-0002-0320-0214], Rendon Restrepo, Augusto [0000-0001-8994-0039], Seyres, Denis [0000-0002-2066-6980], Sun, Ben [0000-0001-6347-2281], Tuna, Salih [0000-0003-3606-4367], Richardson, Sylvia [0000-0003-1998-492X], Vallier, Ludovic [0000-0002-3848-2602], Danesh, John [0000-0003-1158-6791], Soranzo, Nicole [0000-0003-1095-3852], Butterworth, Adam [0000-0002-6915-9015], Turro Bassols, Ernest [0000-0002-1820-6563], Ouwehand, Willem [0000-0002-7744-1790], Astle, William [0000-0001-8866-6672], Downes, Kate [0000-0003-0366-1579], Frontini, Mattia [0000-0001-8074-6299], Apollo - University of Cambridge Repository, MUMC+: MA Med Staf Artsass Interne Geneeskunde (9), RS: CARIM - R1.03 - Cell biochemistry of thrombosis and haemostasis, and Biochemie
- Subjects
Blood Platelets ,GENE-REGULATION ,BLOOD ,Erythroblasts ,Science ,Genetic Variation ,OPEN CHROMATIN ,DISEASE ,Article ,Chromatin ,DNA-BINDING PROTEINS ,THROMBUS FORMATION ,GENOME ,HUMAN CELL-TYPES ,SEQ ,Enhancer Elements, Genetic ,TRAIT LOCI ,Humans ,Promoter Regions, Genetic ,Megakaryocytes - Abstract
Linking non-coding genetic variants associated with the risk of diseases or disease-relevant traits to target genes is a crucial step to realize GWAS potential in the introduction of precision medicine. Here we set out to determine the mechanisms underpinning variant association with platelet quantitative traits using cell type-matched epigenomic data and promoter long-range interactions. We identify potential regulatory functions for 423 of 565 (75%) non-coding variants associated with platelet traits and we demonstrate, through ex vivo and proof of principle genome editing validation, that variants in super enhancers play an important role in controlling archetypical platelet functions., Numerous genetic variants, including those located in the non-coding regions of the genome, are known to be associated with blood cells traits. Here, Frontini and colleagues investigate their potential regulatory functions using epigenomic data and promoter long-range interactions.
- Published
- 2017