Search

Your search keyword '"Tomita, Atsuhiro"' showing total 17 results
Start Over Author "Tomita, Atsuhiro"
17 results on '"Tomita, Atsuhiro"'

1. Class B1 GPCR activation by an intracellular agonist

Author

Kobayashi, Kazuhiro, Kawakami, Kouki, Kusakizako, Tsukasa, Tomita, Atsuhiro, Nishimura, Michihiro, Sawada, Kazuhiro, Okamoto, Hiroyuki H., Hiratsuka, Suzune, Nakamura, Gaku, Kuwabara, Riku, Noda, Hiroshi, Muramatsu, Hiroyasu, Shimizu, Masaru, Taguchi, Tomohiko, Inoue, Asuka, Murata, Takeshi, and Nureki, Osamu

Published
2023
Full Text
View/download PDF

9. Structure and engineering of the minimal type VI CRISPR-Cas13bt3

Author

Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences, Nakagawa, Ryoya, Kannan, Soumya, Altae-Tran, Han, Takeda, Satoru N, Tomita, Atsuhiro, Hirano, Hisato, Kusakizako, Tsukasa, Nishizawa, Tomohiro, Yamashita, Keitaro, Zhang, Feng, Nishimasu, Hiroshi, Nureki, Osamu, Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences, Nakagawa, Ryoya, Kannan, Soumya, Altae-Tran, Han, Takeda, Satoru N, Tomita, Atsuhiro, Hirano, Hisato, Kusakizako, Tsukasa, Nishizawa, Tomohiro, Yamashita, Keitaro, Zhang, Feng, Nishimasu, Hiroshi, and Nureki, Osamu

Abstract

Type VI CRISPR-Cas13 effector enzymes catalyze RNA-guided RNA cleavage and have been harnessed for various technologies, such as RNA detection, targeting, and editing. Recent studies identified Cas13bt3 (also known as Cas13X.1) as a miniature Cas13 enzyme, which can be used for knockdown and editing of target transcripts in mammalian cells. However, the action mechanism of the compact Cas13bt3 remains unknown. Here, we report the structures of the Cas13bt3-guide RNA complex and the Cas13bt3-guide RNA-target RNA complex. The structures revealed how Cas13bt3 recognizes the guide RNA and its target RNA and provided insights into the activation mechanism of Cas13bt3, which is distinct from those of the other Cas13a/d enzymes. Furthermore, we rationally engineered enhanced Cas13bt3 variants and ultracompact RNA base editors. Overall, this study improves our mechanistic understanding of the CRISPR-Cas13 enzymes and paves the way for the development of efficient Cas13-mediated transcriptome modulation technologies.

Published
2023

10. ATP-dependent modulation of MgtE in Mg2+ homeostasis

Author

Tomita, Atsuhiro, Zhang, Mingfeng, Jin, Fei, Zhuang, Wenhui, Takeda, Hironori, Maruyama, Tatsuro, Osawa, Masanori, Hashimoto, Ken-ichi, Kawasaki, Hisashi, Ito, Koichi, Dohmae, Naoshi, Ishitani, Ryuichiro, Shimada, Ichio, Yan, Zhiqiang, Hattori, Motoyuki, and Nureki, Osamu

Published
2017
Full Text
View/download PDF

11. Structural transition of human PTH1R revealed distinct molecular recognition and molecular switch for sustainable activation

Author

Kazuhiro, Kobayashi, primary, Kawakami, Kouki, additional, Kusakizako, Tshukasa, additional, Gono, Hirotake, additional, Tomita, Atsuhiro, additional, Kobayashi, Kan, additional, Shihoya, Wataru, additional, Yamashita, Keitaro, additional, Nishizawa, Tomohiro, additional, Kato, Hideaki, additional, Inoue, Asuka, additional, and Nureki, Osamu, additional

Published
2022
Full Text
View/download PDF

13. Time-resolved serial femtosecond crystallography reveals early structural changes in channelrhodopsin

Author

80547612, 90391979, 60452330, Oda, Kazumasa, Nomura, Takashi, Nakane, Takanori, Yamashita, Keitaro, Inoue, Keiichi, Ito, Shota, Vierock, Johannes, Hirata, Kunio, Maturana, Andrés D, Katayama, Kota, Ikuta, Tatsuya, Ishigami, Itsuki, Izume, Tamaki, Umeda, Rie, Eguma, Ryuun, Oishi, Satomi, Kasuya, Go, Kato, Takafumi, Kusakizako, Tsukasa, Shihoya, Wataru, Shimada, Hiroto, Takatsuji, Tomoyuki, Takemoto, Mizuki, Taniguchi, Reiya, Tomita, Atsuhiro, Nakamura, Ryoki, Fukuda, Masahiro, Miyauchi, Hirotake, Lee, Yongchan, Nango, Eriko, Tanaka, Rie, Tanaka, Tomoyuki, Sugahara, Michihiro, Kimura, Tetsunari, Shimamura, Tatsuro, Fujiwara, Takaaki, Yamanaka, Yasuaki, Owada, Shigeki, Joti, Yasumasa, Tono, Kensuke, Ishitani, Ryuichiro, Hayashi, Shigehiko, Kandori, Hideki, Hegemann, Peter, Iwata, So, Kubo, Minoru, Nishizawa, Tomohiro, Nureki, Osamu, 80547612, 90391979, 60452330, Oda, Kazumasa, Nomura, Takashi, Nakane, Takanori, Yamashita, Keitaro, Inoue, Keiichi, Ito, Shota, Vierock, Johannes, Hirata, Kunio, Maturana, Andrés D, Katayama, Kota, Ikuta, Tatsuya, Ishigami, Itsuki, Izume, Tamaki, Umeda, Rie, Eguma, Ryuun, Oishi, Satomi, Kasuya, Go, Kato, Takafumi, Kusakizako, Tsukasa, Shihoya, Wataru, Shimada, Hiroto, Takatsuji, Tomoyuki, Takemoto, Mizuki, Taniguchi, Reiya, Tomita, Atsuhiro, Nakamura, Ryoki, Fukuda, Masahiro, Miyauchi, Hirotake, Lee, Yongchan, Nango, Eriko, Tanaka, Rie, Tanaka, Tomoyuki, Sugahara, Michihiro, Kimura, Tetsunari, Shimamura, Tatsuro, Fujiwara, Takaaki, Yamanaka, Yasuaki, Owada, Shigeki, Joti, Yasumasa, Tono, Kensuke, Ishitani, Ryuichiro, Hayashi, Shigehiko, Kandori, Hideki, Hegemann, Peter, Iwata, So, Kubo, Minoru, Nishizawa, Tomohiro, and Nureki, Osamu

Abstract

Channelrhodopsins (ChRs) are microbial light-gated ion channels utilized in optogenetics to control neural activity with light . Light absorption causes retinal chromophore isomerization and subsequent protein conformational changes visualized as optically distinguished intermediates, coupled with channel opening and closing. However, the detailed molecular events underlying channel gating remain unknown. We performed time-resolved serial femtosecond crystallographic analyses of ChR by using an X-ray free electron laser, which revealed conformational changes following photoactivation. The isomerized retinal adopts a twisted conformation and shifts toward the putative internal proton donor residues, consequently inducing an outward shift of TM3, as well as a local deformation in TM7. These early conformational changes in the pore-forming helices should be the triggers that lead to opening of the ion conducting pore.

Published
2021

14. The structure of MgtE in the absence of magnesium provides new insights into channel gating

Author

Jin, Fei, primary, Sun, Minxuan, additional, Fujii, Takashi, additional, Yamada, Yurika, additional, Wang, Jin, additional, Maturana, Andrés D., additional, Wada, Miki, additional, Su, Shichen, additional, Ma, Jinbiao, additional, Takeda, Hironori, additional, Kusakizako, Tsukasa, additional, Tomita, Atsuhiro, additional, Nakada-Nakura, Yoshiko, additional, Liu, Kehong, additional, Uemura, Tomoko, additional, Nomura, Yayoi, additional, Nomura, Norimichi, additional, Ito, Koichi, additional, Nureki, Osamu, additional, Namba, Keiichi, additional, Iwata, So, additional, Yu, Ye, additional, and Hattori, Motoyuki, additional

Published
2021
Full Text
View/download PDF

15. Time-resolved serial femtosecond crystallography reveals early structural changes in channelrhodopsin

Author

Oda, Kazumasa, primary, Nomura, Takashi, additional, Nakane, Takanori, additional, Yamashita, Keitaro, additional, Inoue, Keiichi, additional, Ito, Shota, additional, Vierock, Johannes, additional, Hirata, Kunio, additional, Maturana, Andrés D, additional, Katayama, Kota, additional, Ikuta, Tatsuya, additional, Ishigami, Itsuki, additional, Izume, Tamaki, additional, Umeda, Rie, additional, Eguma, Ryuun, additional, Oishi, Satomi, additional, Kasuya, Go, additional, Kato, Takafumi, additional, Kusakizako, Tsukasa, additional, Shihoya, Wataru, additional, Shimada, Hiroto, additional, Takatsuji, Tomoyuki, additional, Takemoto, Mizuki, additional, Taniguchi, Reiya, additional, Tomita, Atsuhiro, additional, Nakamura, Ryoki, additional, Fukuda, Masahiro, additional, Miyauchi, Hirotake, additional, Lee, Yongchan, additional, Nango, Eriko, additional, Tanaka, Rie, additional, Tanaka, Tomoyuki, additional, Sugahara, Michihiro, additional, Kimura, Tetsunari, additional, Shimamura, Tatsuro, additional, Fujiwara, Takaaki, additional, Yamanaka, Yasuaki, additional, Owada, Shigeki, additional, Joti, Yasumasa, additional, Tono, Kensuke, additional, Ishitani, Ryuichiro, additional, Hayashi, Shigehiko, additional, Kandori, Hideki, additional, Hegemann, Peter, additional, Iwata, So, additional, Kubo, Minoru, additional, Nishizawa, Tomohiro, additional, and Nureki, Osamu, additional

Published
2021
Full Text
View/download PDF

16. Author response: Time-resolved serial femtosecond crystallography reveals early structural changes in channelrhodopsin

Author

Oda, Kazumasa, primary, Nomura, Takashi, additional, Nakane, Takanori, additional, Yamashita, Keitaro, additional, Inoue, Keiichi, additional, Ito, Shota, additional, Vierock, Johannes, additional, Hirata, Kunio, additional, Maturana, Andrés D, additional, Katayama, Kota, additional, Ikuta, Tatsuya, additional, Ishigami, Itsuki, additional, Izume, Tamaki, additional, Umeda, Rie, additional, Eguma, Ryuun, additional, Oishi, Satomi, additional, Kasuya, Go, additional, Kato, Takafumi, additional, Kusakizako, Tsukasa, additional, Shihoya, Wataru, additional, Shimada, Hiroto, additional, Takatsuji, Tomoyuki, additional, Takemoto, Mizuki, additional, Taniguchi, Reiya, additional, Tomita, Atsuhiro, additional, Nakamura, Ryoki, additional, Fukuda, Masahiro, additional, Miyauchi, Hirotake, additional, Lee, Yongchan, additional, Nango, Eriko, additional, Tanaka, Rie, additional, Tanaka, Tomoyuki, additional, Sugahara, Michihiro, additional, Kimura, Tetsunari, additional, Shimamura, Tatsuro, additional, Fujiwara, Takaaki, additional, Yamanaka, Yasuaki, additional, Owada, Shigeki, additional, Joti, Yasumasa, additional, Tono, Kensuke, additional, Ishitani, Ryuichiro, additional, Hayashi, Shigehiko, additional, Kandori, Hideki, additional, Hegemann, Peter, additional, Iwata, So, additional, Kubo, Minoru, additional, Nishizawa, Tomohiro, additional, and Nureki, Osamu, additional

Published
2021
Full Text
View/download PDF

17. Cryo-EM structure of the MgtE Mg2+ channel pore domain in Mg2+-free conditions reveals cytoplasmic pore opening

Author

Jin, Fei, primary, Sun, Minxuan, additional, Fujii, Takashi, additional, Yamada, Yurika, additional, Wang, Jin, additional, Maturana, Andrés D., additional, Wada, Miki, additional, Su, Shichen, additional, Ma, Jinbiao, additional, Takeda, Hironori, additional, Kusakizako, Tsukasa, additional, Tomita, Atsuhiro, additional, Nakada-Nakura, Yoshiko, additional, Liu, Kehong, additional, Uemura, Tomoko, additional, Nomura, Yayoi, additional, Nomura, Norimichi, additional, Ito, Koichi, additional, Nureki, Osamu, additional, Namba, Keiichi, additional, Iwata, So, additional, Yu, Ye, additional, and Hattori, Motoyuki, additional

Published
2020
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results