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A resin-bonded bridge (RBB) is a minimally invasive prosthetic treatment option for intact adjacent teeth. However, it is contentious as to whether the mesial or distal adjacent tooth should be used as an abutment. This study aimed to investigate the potential of finite element analysis (FEA) for the selection of abutment teeth and the determination of the optimal design for anterior cantilever RBBs.Three types of RBBs were designed to simulate loss of the maxillary left lateral incisor. The fixed-fixed RBB (FF-RBB) had one retainer each for the left central incisor and canine. Distal- and mesial-cantilever RBBs (D-CRBB and M-CRBB) had a single retainer on the central incisor and canine, respectively. Three-dimensional models for FEA were generated from computed tomography slices and dental casts. FEA was performed for each RBB to evaluate stresses in the intercuspal, protrusive, and lateral mandibular positions.The maximum principal stresses on the bridges in the intercuspal position were 141.9 MPa, 93.6 MPa, and 45.7 MPa, for the FF-RBB, D-CRBB, and M-CRBB, respectively. The stress in the D-CRBB position was greater than in the M-CRBB position in the intercuspal, protrusive, and lateral mandibular positions.Based on the results, M-CRBB on the canine had a higher clinical performance than D-CRBB on the central incisor. FEA was useful for determining the optimal design of RBB for each patient.

Chimpanzees ( Pan troglodytes) with teeth severely damaged by dental caries and/or periodontal disease are often managed with medication and/or tooth extraction. A common endodontic treatment for severely decayed teeth in a 26-year-old female chimpanzee is reported. The left maxillary central incisor tooth had lost its crown, probably due to trauma that was not recent, and it had a fistula most likely due to chronic apical periodontitis. The diagnosis was confirmed radiographically before treatment. To treat the infected root canal, endodontic treatment used in humans was adapted for a chimpanzee. After the treatment, the tooth was sealed using an adhesive resin composite. At 11-years post-treatment, there were no signs of recurrence of the lesion or of failure of the tooth seal. The results of this case report suggest that common endodontic treatments used in humans are also effective in chimpanzees.

We developed a dental fiberscope that can be navigated. As a result we are able to better grasp the device position relative to the teeth, aiming at the lesion more precisely. However, some level of distortion was present, and became more pronounced with wide-angle lenses. Furthermore, the device position and the precise target setting were difficult to consistently ascertain. The aim of this study is to navigate the tip position of the dental fiberscope in the root canal and to improve the navigation system accuracy. We created a dental fiberscope with channels for laser and flooding. A 3D tooth model was made from the raw dental CT data. CT data was imported to image analysis software and segmented based on the CT value. In addition, the optical position of the measurement device, OPTOTRAK system, was used for registration of the 3D model, the actual teeth position, and to chase the scope movement. We developed exclusive software to unify information. The optical distortion in the endoscopic image was numerically rendered to the CT image. We were able to precisely indicate the device position relative to the teeth on the 3D model in the monitor. This allowed us to aim at the lesion more precisely, as the revised endoscopic image matched the 3D model. Consequently, we were more able to grasp the correct position of the endoscope, allowing us to perform laser irradiation on the lesion with a higher degree of safety and precision.Keywords: Fiberscope, Navigation system, Endodontic treatment

Acquiring adequate attached mucosa is important in restoring masticatory function with a removable dental prosthesis or dental implant. In patients with inadequate attached mucosa, a free gingival graft (FGG) with a custom stent is used. However, it is challenging to apply the conventional method for fabricating a stent with a reshaped cast in patients with reconstructed mandibles because the reconstructed mandible has limitations due to a titanium mesh, skin flap, and unique bone morphology. In the present report, we have proposed a new design and fabrication process for a mucocompressive splint via medical engineering to acquire the attached mucosa for the prosthodontic treatment of reconstructed mandibles.Three-dimensional (3D) craniomandibular models were reconstructed from a computed tomography dataset after mandibular reconstruction. The dentition region was replaced with highly precise scan data of the dental casts using a 3D scanner. The alveolar ridge mucosa and teeth were ideally designed using the 3D model while referring to the anatomical structures. The jig used to fabricate the working dental cast with artificial gum in real space was designed and fabricated using a 3D printer. The alveolar ridge was altered into a simulated configuration on the articulator using a jig. A mucocompressive splint was fabricated on an altered dental cast. The splint was immediately seated on the mandible without any major adjustments after the FGG.Adequate attached mucosa for the prosthodontic treatment of a reconstructed mandible was obtained by the pre-surgical fabrication of a mucocompressive splint through medical engineering.

PURPOSE The purpose of this study was to examine the wear of resin materials using two-body wear tests and to verify a selection method for optimal interim restoration materials from findings of a diagnostic occlusal dev ice. METHODS Specimens were prepared from nine different resins used for diagnostic occlusal devices (soft resin) and interim restorations. Wear tests were conducted using an abrasion testing machine. The resulting wear on resin specimens and antagonist stainless-steel styluses was measured using a laser confocal scanning microscope, and the surface conditions were observed through a scanning electron microscope. The data were analyzed with one-way ANOVA and Tukey's multiple comparison test. The findings of the previous study on diagnostic occlusal devices were referred to in order to verify the selection method of optimal interim restoration materials. RESULTS The maximum wear depth of the soft resin specimens was significantly greater than that of the other specimens (p

The longitudinal change in mandibular volume and configuration was quantitatively evaluated in a patient with craniofacial fibrous dysplasia (FD). The influence of mechanical stress on the enlargement that is characteristic of FD was verified via finite element analysis (FEA). The patient was a 38-year-old man diagnosed with craniofacial FD in the mandible. He underwent surgical reduction of FD, but the lesion continued to grow and caused facial asymmetry and masticatory disturbance because of missing teeth. An occlusal overlay removable partial denture was constructed for his lower jaw. Computed tomography (CT) images were acquired 4 times in 10 years, and 3-dimensional (3-D) models were reconstructed from these data. The 3-D models were analyzed volumetrically and morphologically and used for FEA. The FD lesion in the mandible enlarged nonuniformly and had site specificity. From the results of FEA, it is suggested that compression stress induced by the occlusal force through the denture may have influenced the direction of enlargement in FD.

Introduction Displacement of provisional fixed prostheses may result in undesirable and embarrassing outcomes in dental treatments, especially in endodontic treatment. Development of certain counter measures has been necessary to avoid such discomforts. Aim The aim of this report was to propose a pre-endodontic post and core technique to achieve smooth progress of the treatment. Materials and methods The patient was a 59-year-old male diagnosed with an infraocclusion caused by wear of his teeth. He received full mouth provisional fixed restorations for a complete oral rehabilitation. Displacement and fracture of the restorations frequently occurred during the observation period for the function of the restorations. Therefore, the pre-endodontic post and core technique was applied to the abutment teeth before their endodontic treatments were started. The technique consisted of three steps as follows: Step 1: Caries removal and dowel preparation were performed for the abutment teeth having apical periodontitis. Composite cores were indirectly fabricated, which had access holes for endodontic treatment. Step 2: The cores were bonded to the teeth. In endodontic treatment, rubber dam appliances were easily placed owing to the core, and proper tooth isolation was accomplished. Step 3: Fiberposts were bonded to the dowel holes through the access holes after the root canal filling. During endodontic treatment, displacement and/or fracture of the provisional restorations did not occur. Conclusion The pre-endodontic post and core technique was effective in obtaining improved retention of provisional restoration, appropriate isolation for endodontic treatment, and sufficient retention of the post and core. Clinical significance The pre-endodontic post and core technique is useful for avoiding the discomforts in dental treatments, namely, a smooth transition from endodontic to prosthodontic treatment can be achieved.

Purpose This report presents our evaluation system that assesses sleep bruxism. The characteristics and fabrication process of our novel designed splint, and the analysis process of our system are presented. Methods The subjects were 17 volunteers. The splint was fabricated with a self-curing resin compounded with an amino-acid powder for easy wear on the semi-adjustable articulator, and adjusted for a full-balanced occlusion. An impression of the splint, located on the cast, was taken before and after it was worn. The analytical casts were made and scanned via a dental 3D scanner. The datasets were superimposed using two kinds of regions of interest (palate and occlusal surface). The differences between the two datasets were quantitatively presented with pseudo-color mapping. The maximum differences in coronal and apical directions were calculated on the selected area in the occlusal surface when the occlusal surface was used as a region of interest for registration. The relationship between the EMG activities and the change of occlusal surface of the splint were investigated. Results In all subjects, deformation and wear facets on the splint were observed. The differences in the apical direction, which indicate wear depth, were correlated with the maximum muscle activity during sleep (p=0.036). Conclusion From our results, it is suggested that we are not able to eliminate the influence of parafunction for the prosthesis only by designing the surface of occlusal splint using the semi-adjustable articulator. Our splint may have the potential to detect specific facets due to parafunctions as nocturnal bruxism.

Computed tomography (CT) scanning has recently been introduced into forensic medicine and dentistry. However, the presence of metal restorations in the dentition can adversely affect the quality of three-dimensional reconstruction from CT scans. In this study, we aimed to evaluate the reproducibility of a "high-precision, reconstructed 3D model" obtained from a conebeam CT scan of dentition, a method that might be particularly helpful in forensic medicine. We took conebeam CT and helical CT images of three dry skulls marked with 47 measuring points; reconstructed three-dimensional images; and measured the distances between the points in the 3D images with a computer-aided design/computer-aided manufacturing (CAD/CAM) marker. We found that in comparison with the helical CT, conebeam CT is capable of reproducing measurements closer to those obtained from the actual samples. In conclusion, our study indicated that the image-reproduction from a conebeam CT scan was more accurate than that from a helical CT scan. Furthermore, the "high-precision reconstructed 3D model" facilitates reliable visualization of full-sized oral and maxillofacial regions in both helical and conebeam CT scans.

Three strains TKU9, TKU49 and TKU50(T) , were isolated from the oral cavities of chimpanzees (Pan troglodytes). The isolates were all gram-positive, facultative anaerobic cocci that lacked catalase activity. Analysis of partial 16S rRNA gene sequences showed that the most closely related species was Streptococcus infantis (96.7%). The next most closely related species to the isolates were S. rubneri, S. mitis, S. peroris and S. australis (96.6 to 96.4%). Based on the rpoB and gyrB gene sequences, TKU50(T) was clustered with other member of the mitis group. Enzyme activity and sugar fermentation patterns differentiated this novel bacterium from other members of the mitis group streptococci. The DNA G + C content of strain TKU50(T) was 46.7 mol%, which is the highest reported value for members of the mitis group (40-46 mol%). On the basis of the phenotypic characterization, partial 16S rRNA gene and sequences data for two housekeeping gene (gyrB and rpoB), we propose a novel taxa, S. panodentis for TKU 50(T) (type strain = CM 30579(T) = DSM 29921(T) ), for these newly described isolates.

The objective of this study is to evaluate usefulness of mandibular reconstructions using a custom-made titanium mesh (Ti-mesh) tray and particulate cancellous bone and marrow (PCBM). A consecutive nine patients who underwent mandibular reconstruction were enrolled in this study. They were five men and four women (mean age: 53.9 years). Virtual reality simulation was performed using computer software based on the pre-operative computed tomography data. A 3-dimensional (3-D) skull model was constructed using a 3-D printer. A tray was custom-made from a Ti-mesh sheet bent to adapt to the model. After PCBM harvesting from bilateral posterior ilia, the tray was fixed to the host bone. New bone formation and configuration of the reconstructed mandible were assessed radiologically. Complications were recorded in each patient during the follow-up period. Patients, satisfaction with post-operative facial contour was evaluated using a visual analogue scale (VAS score, range = 0-100). In six of nine patients, excellent new bone formation was recognised and expected results were radiologically achieved. Complications occurred in four patients. These complications included Ti-mesh fracture, Ti-mesh exposure in the oral cavity, and delayed infection. Mean VAS score on patient satisfaction was 77.6. Although the data are preliminary, the results suggest that this method is clinically useful.

Six strains, TKU 25, TKU 28, TKU 30, TKU 31T, TKU 33 and TKU 34, were isolated from the oral cavity of a chimpanzee (Pan troglodytes). Colonies of strains grown on Mitis–Salivarius agar were similar in morphology to that of Streptococcus mutans . The novel strains were Gram-stain-positive, facultatively anaerobic cocci that lacked catalase activity. Analysis of the partial 16S rRNA gene sequences of these isolates showed that the most closely related strain was the type strain of S. mutans (96.4 %). The next closely related strains to the isolates were the type strains of Streptococcus devriesei (94.5 %) and Streptococcus downei (93.9 %). These isolates could be distinguished from S. mutans by inulin fermentation and alkaline phosphatase activity (API ZYM system). The peptidoglycan type of the novel isolates was Glu–Lys–Ala3. Strains were not susceptible to bacitracin. On the basis of phenotypic characterization, partial 16S rRNA gene and two housekeeping gene (groEL and sodA) sequence data, we propose a novel taxon, Streptococcus troglodytae sp. nov.; the type strain is TKU 31T ( = JCM 18038T = DSM 25324T).

The objective of this study was to evaluate usefulness of mandibular reconstructions using custom-made titanium mesh (Ti-mesh) tray and particulate cancellous bone and marrow (PCBM). Consecutive 21 patients who underwent mandibular reconstruction were enrolled in this study. They were 13 men and 8 women (mean age, 52.0 years). Virtual reality simulation was performed using computer software based on the preoperative computed tomography data. A 3-dimensional skull model was constructed using 3-dimensional printer. A tray was custom-made from Ti-mesh sheet bent to adapt to the model. After PCBM harvesting from posterior ilia and/or proximal tibia, the tray was fixed to the host bone. New bone formation and configuration of the reconstructed mandible were assessed radiologically. Complications were recorded in each patient during the follow-up period. Patients' satisfaction with postoperative facial contour was evaluated using visual analog scale (VAS score, range, 0-100). In 16 of 21 patients, excellent new bone formation was recognized and expected results were radiologically achieved. In 5 patients, new bone formation was insufficient. Causes of insufficient bone formation included postoperative infection in 2 patients, Ti-mesh tray fracture in 2 patients, and local recurrence of lower gingival cancer in 1 patient. To prevent a tray fracture, a double-layered Ti-mesh tray was useful. Mean VAS score on patients' satisfaction was 77.1. Our results comprehensively suggest that mandibular reconstruction using custom-made Ti-mesh tray and PCBM is clinically useful.

The purpose of this present study was to improve student knowledge on the semi-adjustable articulator. Following from the insights provided by Ryan and Deci’s review of Organismic Integration Theory (OIT), we attempted to promote student interest in the semi-adjustable articulator. OIT was also utilized to provide guidance in assessing student motivation and its relation between the degree of student knowledge/interest. Finally, the study also aimed at establishing a rationale to evaluate and further develop our classroom practices at the institute. Through this process for raising student awareness, it was suggested that to observe the FGPs was useful to recognize the utility of a semi-adjustable articulator when a bridge at the molar region is made. Moreover, it was suggested that the student motivation helped promote their understanding. This highlights the need to support student motivation in our classroom. Finally, future studies on how professors perceive bureaucratic pressures from Ministry of Health, Labor and Welfare (MHLW) and within the institute could add additional insights to improving our educational practices in the classroom.

To elucidate the influences of obesity on the properties and volume of lingual (genioglossus and geniohyoid) muscles in obese rats.We analysed the accumulation of triacylglycerol and the diameter of myofibres in the lingual muscles using histochemistry, and the MyHC composition using real-time PCR in rats fed a high-fat diet for 10 weeks. In the genioglossus and geniohyoid muscles, the percentage of oil droplet areas in the obesity group were 3.6 and 2.5 times greater than those in the control group, respectively (p0.025). The diameters of the slow myofibres in the genioglossus and geniohyoid muscles were approximately 20% greater in the obesity group than in the control group (p0.0001), while that of the fast myofibres in the geniohyoid muscle was approximately 10% greater in the obesity group than in the control group (p0.0001). No significant difference in the expressions of any of the MyHC isoforms studied was found in any of the muscles studied between the obesity and control groups.High-fat diet feeding induced the fat deposition in the myofibre and influenced the structure of the lingual (genioglossus and geniohyoid) muscles.

We compared the hypertrophic effects of clenbuterol, a β2-adrenergic agonist, on the masseter, digastric, and temporalis with those on the tongue, tibialis anterior, soleus, diaphragm, and heart. The weights of masseter, digastric and temporalis in the clenbuterol group were 36 ~ 56% greater than those in the control group, whereas those of the tibialis anterior, diaphragm, and heart weights in the clenbuterol group were 9 ~ 33% greater than those in the control group. No significant difference in the weights of the soleus and tongue was found between the control and clenbuterol groups. Taken together with our present and previously reported results, it is suggested that the hypertrophic effects of clenbuterol on the masseter, digastric, and temporalis are greater than those on the limb, trunk, and heart.

Cellular diversity of the brain is largely attributed to the spatial and temporal heterogeneity of progenitor cells. In mammalian cerebral development,it has been difficult to determine how heterogeneous the neural progenitor cells are, owing to dynamic changes in their nuclear position and gene expression. To address this issue, we systematically analyzed the cDNA profiles of a large number of single progenitor cells at the mid-embryonic stage in mouse. By cluster analysis and in situ hybridization, we have identified a set of genes that distinguishes between the apical and basal progenitors. Despite their relatively homogeneous global gene expression profiles, the apical progenitors exhibit highly variable expression patterns of Notch signaling components, raising the possibility that this causes the heterogeneous division patterns of these cells. Furthermore, we successfully captured the nascent state of basal progenitor cells. These cells are generated shortly after birth from the division of the apical progenitors, and show strong expression of the major Notch ligand delta-like 1, which soon fades away as the cells migrate in the ventricular zone. We also demonstrated that attenuation of Notch signals immediately induces differentiation of apical progenitors into nascent basal progenitors. Thus, a Notch-dependent feedback loop is likely to be in operation to maintain both progenitor populations.

Objective The objective of this study was to investigate the difference of neck fat tissue distribution by sex and its correlation with retropalatal and retroglossal airway. Study design A total of 38 consecutive patients (male: 19; female: 19) who received a CT scan were compared in the retroglossal region and at the narrowest cross section of the airway. Retroglossal fat tissue volume (FV) was segmented with Amira software and separated into subcutaneous and internal fat volume (SFV, IFV). These volumes were normalized by retroglossal neck volume (NV). Results Men had 51.9% more IFV/NV and 64.4% less SFV/NV compared with women. Age-adjusted BMI was negatively correlated with retroglossal airway volume (normalized by NV) and with the lateral width of the smallest cross-section airway (LW) in females. In males the IFV/NV was negatively correlated with LW, after adjusting for BMI and age. Conclusion Upper airway collapsibility analysis is needed to rule out whether increased BMI or IFV causes an increase in airway collapsibility.

Three strains TKU9, TKU49 and TKU50(T) , were isolated from the oral cavities of chimpanzees (Pan troglodytes). The isolates were all gram-positive, facultative anaerobic cocci that lacked catalase activity. Analysis of partial 16S rRNA gene sequences showed that the most closely related species was Streptococcus infantis (96.7%). The next most closely related species to the isolates were S. rubneri, S. mitis, S. peroris and S. australis (96.6 to 96.4%). Based on the rpoB and gyrB gene sequences, TKU50(T) was clustered with other member of the mitis group. Enzyme activity and sugar fermentation patterns differentiated this novel bacterium from other members of the mitis group streptococci. The DNA G + C content of strain TKU50(T) was 46.7 mol%, which is the highest reported value for members of the mitis group (40-46 mol%). On the basis of the phenotypic characterization, partial 16S rRNA gene and sequences data for two housekeeping gene (gyrB and rpoB), we propose a novel taxa, S. panodentis for TKU 50(T) (type strain = CM 30579(T) = DSM 29921(T) ), for these newly described isolates.

Purpose The purpose of this report is to propose a computer assisted mandibular reconstruction procedure, utilizing a custom-made Ti-mesh tray with particulate cancellous bone and marrow, and a removable denture. This procedure was based on the top-down treatment technique, and reviews the case of a representative patient with mandibular continuity defect. Methods The patient was a 74-year old female with a chief complaint of facial asymmetry and masticatory dysfunction. Due to gingival carcinoma, she underwent a segmental mandibulectomy on the left mandibule. On the VR space, using 3-D reconstructed computer tomography data, the residual right-side mandibular fragment was repositioned based on the condylar position and the occlusal relation. The mandibular fragment was then mirrored for a central sagittal plane. The position of the mirrored object was slightly arranged with the occlusal relation. Through the above operations, the landmark configuration, for the custom-made Ti-mesh tray as a virtual simulation model, was fabricated. On the physical model, we produced a custom-made Ti-mesh tray with a commercial Ti-mesh sheet. Surgical treatment was carried out using the tray. The denture pattern was designed by a dental technician on the VR space, fabricated using a 3D printer, and modified to create an impression tray with resin. Using the impression, the temporary removable denture was fabricated. Conclusions We propose a computer assisted design for a custom-made Ti-mesh tray and a removable denture, based on the Top-down treatment concept. We feel this technique is advantageous in reconstructing functional occlusion, and in accurately regaining dental and facial esthetics.

The purpose of this study was to observe the variations of cervical curvature in patients with infraocclusion, and to compare this with the controls. In this study, the infraocclusion criteria were defined with the Pr-id as